RchiOBHm_Chr2g0102901

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000947
Physical Location & Seq
Forward (+)
14526127 .. 14527503
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47730

Sequence Viewer

Length: 1377 bp
ATGCCTGCCTCTCTCTTCTCTTCTATTGTATATAAGTGCTTTTTTAACATCAGAATTGATTTGGGTATGGGGAATTTATGTGAAAATTTGTGGATTGAGATATTGGGTAGGTTAACCTCGGAGAAGGACTTGGTGCCATGCATGAGTGTGTCCAAGTTATGGCACCGAATCACATCGGATCTCTGGGTGGGAAAATTTTGGGCGCAGTCCCCTCCTTTGGGTCTTTTCTTGCGCACCGCCGCCAACACCACCGTGGGCAATCTAAAATATCATTGCATGAATTATATCTCTTTATATAATTATCATGACATAGAAGATAACCTGGAAAGCACCCGCGTTTTCGTTTTCGGACAACCATATTATGACATAGAAGATTATAGGGACCAAAAGATGATTTTGCAGACTTTTGGTAGAGAATGGCATTATCCGGGTTATTATGATGATGACGAGGATGATGGTGTTCTTGATGATGATTATAAACCCGCTGATCGTGATCAGTACCTAAACGGTTGTAATGGGTTACTTTTGTTATTGAAATCAGCAACTCATCAATTTTATGTTTGCAATCCCATCACAAGACAACAAGTCGCCATACCTAAAGCCCGTGTGCACAAAAACCATGATGATCAGCATTTTTGTGCTGCCTTGGCTTTTGACCCTTCTGAATCTCCTCATCACTACAGAATTGTGAGAATTGATTTTTCTCCGGAACCGGCTTCATCAGCCTCAGACTCCTCATCCTTGATAGACATATTTTCGTCCGAATATGGACAATGGATTCGACATAGGTTGCAGCTTGATTCAAGTCTCACTGACGGGTTGAAAGAGTGCAAGTTGTGCAGGCAATTTTTTTATTTGCGGGGAAAGTTATACAGTATAACCATGTCGTGGAACCTCCTATGCATTGACCTCAAGACTGTTGAAGCTTCCGCCCTTGAACTTCCTATCCCGGTGGCGGATAGAACCGGAGCCATGGGATGTCTTGGGGTGTCAAGGGACCTTCTTTGCTATATGAAGCGTATGCCTGATAAATCAAGTCGGTTTCACAATCTGGTAGTTTGGTCTTATGAAAAAGGTGAGTGGATTCCCCGATATAGTGTAAGTTGTCATTTGTTGGGAGAAGGAATATTGGATGATCTAGGCTATGACTACTATGACACCATGGAACCCTATGCAATAAGCCCAAGTTCGGATCTCTTGTTCTTTGGCACTCCAACCTTGATTTACTGTATCCACCTTAAAAGCGAAAATATGAAATTCGTTGGTGAAACGAGCTGTAAAATAGATACACCTGCTTGTTTCTTGACATTACGTGCTTGCTTCGTACCTTTCCACACTCAATTGCGTAAGGGGAACCACGTCTCCATTTCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

458

Amino Acids

52.69

Weight (kDa)

5.72

Isoelectric Point (pI)

52.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 162 - 317 1.7e-06 KIB1-4 beta-propeller
FBA_1 PF07734 166 - 343 1e-09 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 167 - 361 6.1e-11 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 477
AarI CACCTGC 1 cut(s) 1300
Acc16I TGCGCA 1 cut(s) 233
Acc36I ACCTGC 1 cut(s) 1300
AccB1I GGYRCC 2 cut(s) 133, 162
AccB7I CCANNNNNTGG 2 cut(s) 159, 888
AccII CGCG 1 cut(s) 336
AccIII TCCGGA 1 cut(s) 706
AciI CCGC 7 cut(s) 237, 240, 334, 483, 859, 930, 956
AclWI GGATC 2 cut(s) 186, 1200
AcsI RAATTY 4 cut(s) 73, 85, 194, 1256
AfaI GTAC 2 cut(s) 500, 1326
AfiI CCNNNNNNNGG 6 cut(s) 159, 217, 218, 888, 955, 1189
AgsI TTSAA 5 cut(s) 535, 804, 823, 923, 938
AhdI GACNNNNNGTC 1 cut(s) 584
AjiI CACGTC 1 cut(s) 1360
AjnI CCWGG 1 cut(s) 321
AleI CACNNNNGTG 1 cut(s) 251
AloI GAACNNNNNNTCC 5 cut(s) 930, 962, 1184, 1216, 1346
AluBI AGCT 3 cut(s) 796, 926, 1275
AluI AGCT 3 cut(s) 796, 926, 1275
Alw21I GWGCWC 1 cut(s) 612
Alw26I GTCTC 2 cut(s) 812, 1366
Alw44I GTGCAC 1 cut(s) 608
AlwI GGATC 2 cut(s) 186, 1200
Aor13HI TCCGGA 1 cut(s) 706
ApaLI GTGCAC 1 cut(s) 608
ApeKI GCWGC 2 cut(s) 641, 793
ApoI RAATTY 4 cut(s) 73, 85, 194, 1256
ArsI GACNNNNNNTTYG 2 cut(s) 762, 794
AspLEI GCGC 2 cut(s) 205, 234
AspS9I GGNCC 2 cut(s) 382, 997
AsuC2I CCSGG 2 cut(s) 429, 950
AsuHPI GGTGA 2 cut(s) 1088, 1277
AvaII GGWCC 2 cut(s) 382, 997
BaeGI GKGCMC 1 cut(s) 612
BanI GGYRCC 2 cut(s) 133, 162
Bbv12I GWGCWC 1 cut(s) 612
BbvI GCAGC 2 cut(s) 628, 805
BccI CCATC 2 cut(s) 449, 578
BciT130I CCWGG 1 cut(s) 323
BciVI GTATCC 1 cut(s) 1241
BclI TGATCA 2 cut(s) 493, 625
BcnI CCSGG 2 cut(s) 429, 950
BcoDI GTCTC 2 cut(s) 812, 1366
BfaI CTAG 1 cut(s) 1139
BfmI CTRYAG 1 cut(s) 679
BfuAI ACCTGC 1 cut(s) 1300
BfuI GTATCC 1 cut(s) 1241
BisI GCNGC 3 cut(s) 240, 642, 794
BlsI GCNGC 3 cut(s) 241, 643, 795
Bme1390I CCNGG 3 cut(s) 323, 429, 950
Bme18I GGWCC 2 cut(s) 382, 997
BmeRI GACNNNNNGTC 1 cut(s) 584
BmgBI CACGTC 1 cut(s) 1360
BmgT120I GGNCC 2 cut(s) 382, 997
BmiI GGNNCC 9 cut(s) 135, 164, 383, 711, 893, 970, 998, 1167, 1355
BmrFI CCNGG 3 cut(s) 323, 429, 950
BpuEI CTTGAG 1 cut(s) 896
BpuMI CCSGG 2 cut(s) 429, 950
BsaAI YACGTR 1 cut(s) 1313
BsaBI GATNNNNATC 1 cut(s) 492
BsaJI CCNNGG 5 cut(s) 117, 252, 645, 972, 1161
BsaWI WCCGGW 2 cut(s) 706, 965
BsaXI ACNNNNNCTCC 2 cut(s) 1346, 1376
Bsc4I CCNNNNNNNGG 6 cut(s) 159, 217, 218, 888, 955, 1189
Bse118I RCCGGY 1 cut(s) 712
Bse3DI GCAATG 1 cut(s) 271
Bse8I GATNNNNATC 1 cut(s) 492
BseAI TCCGGA 1 cut(s) 706
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 5 cut(s) 117, 252, 645, 972, 1161
BseGI GGATG 4 cut(s) 457, 737, 983, 1138
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 6 cut(s) 159, 217, 218, 888, 955, 1189
BseMI GCAATG 1 cut(s) 271
BseMII CTCAG 1 cut(s) 741
BseRI GAGGAG 2 cut(s) 660, 724
BseSI GKGCMC 1 cut(s) 612
BseXI GCAGC 2 cut(s) 628, 805
BsgI GTGCAG 1 cut(s) 859
Bsh1236I CGCG 1 cut(s) 336
BshNI GGYRCC 2 cut(s) 133, 162
BsiHKAI GWGCWC 1 cut(s) 612
BsiSI CCGG 5 cut(s) 428, 707, 713, 950, 966
BslFI GGGAC 3 cut(s) 193, 395, 1010
BslI CCNNNNNNNGG 6 cut(s) 159, 217, 218, 888, 955, 1189
BsmAI GTCTC 2 cut(s) 812, 1366
BsmBI CGTCTC 1 cut(s) 1366
BsmFI GGGAC 3 cut(s) 193, 395, 1010
Bsp1286I GDGCHC 1 cut(s) 612
Bsp13I TCCGGA 1 cut(s) 706
Bsp143I GATC 6 cut(s) 178, 487, 493, 625, 1135, 1192
Bsp19I CCATGG 2 cut(s) 972, 1161
BspACI CCGC 7 cut(s) 237, 240, 334, 483, 859, 930, 956
BspCNI CTCAG 1 cut(s) 740
BspEI TCCGGA 1 cut(s) 706
BspFNI CGCG 1 cut(s) 336
BspHI TCATGA 1 cut(s) 304
BspLI GGNNCC 9 cut(s) 135, 164, 383, 711, 893, 970, 998, 1167, 1355
BspMI ACCTGC 1 cut(s) 1300
BspPI GGATC 2 cut(s) 186, 1200
BspT107I GGYRCC 2 cut(s) 133, 162
BsrDI GCAATG 1 cut(s) 271
BsrFI RCCGGY 1 cut(s) 712
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 5 cut(s) 117, 252, 645, 972, 1161
BssMI GATC 6 cut(s) 178, 487, 493, 625, 1135, 1192
BssT1I CCWWGG 3 cut(s) 645, 972, 1161
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 5 cut(s) 253, 509, 875, 919, 1229
Bst6I CTCTTC 2 cut(s) 20, 25
BstAPI GCANNNNNTGC 1 cut(s) 837
BstBAI YACGTR 1 cut(s) 1313
BstC8I GCNNGC 3 cut(s) 6, 842, 1318
BstDEI CTNAG 1 cut(s) 727
BstDSI CCRYGG 3 cut(s) 252, 972, 1161
BstF5I GGATG 4 cut(s) 457, 737, 983, 1138
BstFNI CGCG 1 cut(s) 336
BstHHI GCGC 2 cut(s) 205, 234
BstKTI GATC 6 cut(s) 181, 490, 496, 628, 1138, 1195
BstMAI GTCTC 2 cut(s) 812, 1366
BstMBI GATC 6 cut(s) 178, 487, 493, 625, 1135, 1192
BstMWI GCNNNNNNNGC 3 cut(s) 647, 722, 837
BstNI CCWGG 1 cut(s) 323
BstSCI CCNGG 3 cut(s) 321, 427, 948
BstSFI CTRYAG 1 cut(s) 679
BstSLI GKGCMC 1 cut(s) 612
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 2 cut(s) 628, 805
BstX2I RGATCY 2 cut(s) 178, 1192
BstYI RGATCY 2 cut(s) 178, 1192
BsuI GTATCC 1 cut(s) 1241
BtgI CCRYGG 3 cut(s) 252, 972, 1161
BtrI CACGTC 1 cut(s) 1360
BtsCI GGATG 4 cut(s) 457, 737, 983, 1138
BtsIMutI CAGTG 1 cut(s) 810
BveI ACCTGC 1 cut(s) 1300
Cac8I GCNNGC 3 cut(s) 6, 842, 1318
CciI TCATGA 1 cut(s) 304
CfoI GCGC 2 cut(s) 205, 234
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 2 cut(s) 382, 997
Csp6I GTAC 2 cut(s) 499, 1325
CviAII CATG 8 cut(s) 138, 142, 277, 305, 620, 883, 973, 1162
CviQI GTAC 2 cut(s) 499, 1325
DdeI CTNAG 1 cut(s) 727
DpnI GATC 6 cut(s) 180, 489, 495, 627, 1137, 1194
DpnII GATC 6 cut(s) 178, 487, 493, 625, 1135, 1192
DriI GACNNNNNGTC 1 cut(s) 584
Eam1104I CTCTTC 2 cut(s) 20, 25
Eam1105I GACNNNNNGTC 1 cut(s) 584
EarI CTCTTC 2 cut(s) 20, 25
EciI GGCGGA 2 cut(s) 919, 971
Eco130I CCWWGG 3 cut(s) 645, 972, 1161
Eco47I GGWCC 2 cut(s) 382, 997
EcoO109I RGGNCCY 1 cut(s) 997
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 3 cut(s) 645, 972, 1161
EcoT22I ATGCAT 2 cut(s) 143, 905
ErhI CCWWGG 3 cut(s) 645, 972, 1161
Esp3I CGTCTC 1 cut(s) 1366
FaeI CATG 8 cut(s) 141, 145, 280, 308, 623, 886, 976, 1165
FaqI GGGAC 3 cut(s) 193, 395, 1010
FatI CATG 8 cut(s) 137, 141, 276, 304, 619, 882, 972, 1161
FauI CCCGC 3 cut(s) 341, 490, 852
FbaI TGATCA 2 cut(s) 493, 625
Fnu4HI GCNGC 3 cut(s) 240, 642, 794
FokI GGATG 4 cut(s) 464, 724, 990, 1145
Fsp4HI GCNGC 3 cut(s) 240, 642, 794
FspBI CTAG 1 cut(s) 1139
FspI TGCGCA 1 cut(s) 233
GlaI GCGC 2 cut(s) 204, 233
GluI GCNGC 3 cut(s) 240, 642, 794
HapII CCGG 5 cut(s) 428, 707, 713, 950, 966
HhaI GCGC 2 cut(s) 205, 234
Hin1II CATG 8 cut(s) 141, 145, 280, 308, 623, 886, 976, 1165
Hin6I GCGC 2 cut(s) 203, 232
HinP1I GCGC 2 cut(s) 203, 232
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 924
HinfI GANTC 6 cut(s) 168, 665, 731, 778, 800, 1084
HpaI GTTAAC 1 cut(s) 114
HpaII CCGG 5 cut(s) 428, 707, 713, 950, 966
HphI GGTGA 2 cut(s) 1088, 1277
Hpy166II GTNNAC 2 cut(s) 114, 610
Hpy188I TCNGA 8 cut(s) 53, 121, 178, 350, 664, 730, 763, 1192
Hpy188III TCNNGA 6 cut(s) 305, 464, 491, 707, 913, 1303
Hpy8I GTNNAC 2 cut(s) 114, 610
HpyAV CCTTC 4 cut(s) 118, 669, 1010, 1115
HpyCH4III ACNGT 5 cut(s) 253, 509, 875, 919, 1229
HpyCH4IV ACGT 2 cut(s) 1312, 1359
HpyF10VI GCNNNNNNNGC 3 cut(s) 647, 722, 837
HpyF3I CTNAG 1 cut(s) 727
HpySE526I ACGT 2 cut(s) 1312, 1359
Hsp92II CATG 8 cut(s) 141, 145, 280, 308, 623, 886, 976, 1165
HspAI GCGC 2 cut(s) 203, 232
Kpn2I TCCGGA 1 cut(s) 706
Ksp22I TGATCA 2 cut(s) 493, 625
KspAI GTTAAC 1 cut(s) 114
Kzo9I GATC 6 cut(s) 178, 487, 493, 625, 1135, 1192
LmnI GCTCC 1 cut(s) 968
Lsp1109I GCAGC 2 cut(s) 628, 805
MaeI CTAG 1 cut(s) 1139
MaeII ACGT 2 cut(s) 1312, 1359
MaeIII GTNAC 1 cut(s) 519
MalI GATC 6 cut(s) 180, 489, 495, 627, 1137, 1194
MboI GATC 6 cut(s) 178, 487, 493, 625, 1135, 1192
MboII GAAGA 4 cut(s) 7, 12, 326, 383
MfeI CAATTG 1 cut(s) 1340
MflI RGATCY 2 cut(s) 178, 1192
MhlI GDGCHC 1 cut(s) 612
MlyI GAGTC 1 cut(s) 725
MmeI TCCRAC 1 cut(s) 1238
MnlI CCTC 9 cut(s) 19, 127, 222, 442, 681, 736, 745, 905, 920
Mph1103I ATGCAT 2 cut(s) 143, 905
MroI TCCGGA 1 cut(s) 706
MseI TTAA 3 cut(s) 45, 113, 1239
MslI CAYNNNNRTG 3 cut(s) 146, 251, 636
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 5 cut(s) 428, 707, 713, 950, 966
MspR9I CCNGG 3 cut(s) 323, 429, 950
MunI CAATTG 1 cut(s) 1340
MvaI CCWGG 1 cut(s) 323
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 3 cut(s) 647, 722, 837
NciI CCSGG 2 cut(s) 429, 950
NcoI CCATGG 2 cut(s) 972, 1161
NdeII GATC 6 cut(s) 178, 487, 493, 625, 1135, 1192
NlaIII CATG 8 cut(s) 141, 145, 280, 308, 623, 886, 976, 1165
NlaIV GGNNCC 9 cut(s) 135, 164, 383, 711, 893, 970, 998, 1167, 1355
NsbI TGCGCA 1 cut(s) 233
NsiI ATGCAT 2 cut(s) 143, 905
OliI CACNNNNGTG 1 cut(s) 251
PagI TCATGA 1 cut(s) 304
PaqCI CACCTGC 1 cut(s) 1300
PfeI GAWTC 5 cut(s) 168, 665, 778, 800, 1084
PflMI CCANNNNNTGG 2 cut(s) 159, 888
PkrI GCNGC 3 cut(s) 241, 643, 795
PleI GAGTC 1 cut(s) 725
PpsI GAGTC 1 cut(s) 725
Ppu21I YACGTR 1 cut(s) 1313
PpuMI RGGWCCY 1 cut(s) 997
PsiI TTATAA 1 cut(s) 477
Psp5II RGGWCCY 1 cut(s) 997
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 9 cut(s) 135, 164, 383, 711, 893, 970, 998, 1167, 1355
PspPI GGNCC 2 cut(s) 382, 997
PspPPI RGGWCCY 1 cut(s) 997
PsuI RGATCY 2 cut(s) 178, 1192
RsaI GTAC 2 cut(s) 500, 1326
RsaNI GTAC 2 cut(s) 499, 1325
RseI CAYNNNNRTG 3 cut(s) 146, 251, 636
SaqAI TTAA 3 cut(s) 45, 113, 1239
SatI GCNGC 3 cut(s) 240, 642, 794
Sau3AI GATC 6 cut(s) 178, 487, 493, 625, 1135, 1192
Sau96I GGNCC 2 cut(s) 382, 997
SchI GAGTC 1 cut(s) 725
ScrFI CCNGG 3 cut(s) 323, 429, 950
SduI GDGCHC 1 cut(s) 612
SfcI CTRYAG 1 cut(s) 679
SinI GGWCC 2 cut(s) 382, 997
SmiMI CAYNNNNRTG 3 cut(s) 146, 251, 636
SmlI CTYRAG 1 cut(s) 911
SmoI CTYRAG 1 cut(s) 911
SsiI CCGC 7 cut(s) 237, 240, 334, 483, 859, 930, 956
SspI AATATT 1 cut(s) 1128
SspMI CTAG 1 cut(s) 1139
StyD4I CCNGG 3 cut(s) 321, 427, 948
StyI CCWWGG 3 cut(s) 645, 972, 1161
TaaI ACNGT 5 cut(s) 253, 509, 875, 919, 1229
TaiI ACGT 2 cut(s) 1315, 1362
TaqI TCGA 1 cut(s) 781
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 5 cut(s) 168, 665, 778, 800, 1084
Tru1I TTAA 3 cut(s) 45, 113, 1239
Tru9I TTAA 3 cut(s) 45, 113, 1239
TscAI CASTG 1 cut(s) 817
TseI GCWGC 2 cut(s) 641, 793
TspDTI ATGAA 6 cut(s) 293, 708, 1028, 1083, 1268, 1359
TspRI CASTG 1 cut(s) 817
Van91I CCANNNNNTGG 2 cut(s) 159, 888
VneI GTGCAC 1 cut(s) 608
VpaK11BI GGWCC 2 cut(s) 382, 997
XapI RAATTY 4 cut(s) 73, 85, 194, 1256
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XspI CTAG 1 cut(s) 1139
Zsp2I ATGCAT 2 cut(s) 143, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.