Rh2CG163500

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000947
Physical Location & Seq
Forward (+)
14465528 .. 14513435
47908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG163500.1

Sequence Viewer

Length: 1368 bp
ATGGGGGATTTATGCGAGGATTTGTGGGTTGAGATTTTGGGTAGACTATCATCGGAGAAGGACTTGGTGCCATGCATGAGCGTGTCCAAGTTATGGCACCGAATAACATCTGGTCTTTGGGTGCAGAGATTTTGGGCGCAGTCCCCTCCTTTGGGTCTTTTCTTCCGCACCGCCACCACCAGCACCACCACCGTAGACAAGGTATTGCATCGTATGAATTATATCTCTTTATATAATTATCATGCCATGGAAGATAACCTGGAAAGCACTCGTTCTTGCGGAGGTCAATTTTATGACGAAGAATCGTATAGGACGAAAAAGTCTCATTGGCAGTATTTTGATCGACAATGGTATAAGCCGGGTGCCACTGCTGAATCCACTGATCGTGATGAGTACCTAAATGGTTGCAATGGTTTAGTTTTGTTAATGAAATCAGCAACTTATCAGTTTTATGTTTGCAATCCCATCACAAGACAACAAGTTGCCATACCTAAAGCCTGTGTCCACCAAAACCATGATAATCAGCATTTTTGTGCTGCTTTGGCTTTTGACCCTTCCGAATCTGCTCATCACTTCAGAGTTGTTAGAATTGATTATTCTGCATCCTACTCAGCTGTGCTGGATATATTCTTGACTGAATATGGACTGTGGGTTCGACATTGGCTGCAAATTGACCCAGGTTTTACTGAGGGATTCAAAAGCTCCAAGCTGTGCAGGCATTTTGTTTACTTGCGAGGTAGGCTATACAGCATAGCCCTATCCTGGAACCTTTTATGCATTGACCTCAATACTGTTGCAGCTTCTGCCCTTGAACTTCCTGTTCCAGAAGATGAAAAGACCGGAGCCATGGGATGTCTTGGGGTGTCGATGAATAGTCTCTGCTATATGAAGCGAATGCTCGATAATTCGAAGGACTTTCACAATCTGGCAGTTTGGTATAATGTTGAAGAATCTGGGGAATGGATTCTCAGATATAGTGTTTCTTGTCGCTTATTGGGATACGGATTCCAGTCTCGAGGTTATGACTACGATGATTCCATGGAACCCTGTGCAATAAGTCCAACTTCAGATCTCTTGTTCTATGGTAATCCCAACCTGATTTGTTGTATCCACCTTAAAAGCGAAAAGATGAAATTCGTTTGCGAAACAATCTCTAAAATAGATCCACCTGCTTCTTTCTTGACATTACGTGCATGCTTCGTACCTTTCTACACTCAGTTGAACAAGAGGAATGACGTCTCCACTCCATTTTTTGCTCATTTGGTGAATCTTGGACTCTTGGTTATGTGGGAACAGGGAACTGAAAAGTCCACAAATTTGGAGTCTTATAAGTTTCAAATCATTTCCAAGCACGACACTTGGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

455

Amino Acids

52.47

Weight (kDa)

6.21

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 128 - 283 3.1e-08 KIB1-4 beta-propeller
b-prop_At3g26010-like PF24750 129 - 333 3.5e-11 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 135 - 312 2.1e-08 F-box associated beta propeller domain
Beta-prop_AT5G49610-like PF23635 136 - 384 4.7e-07 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1329
AarI CACCTGC 1 cut(s) 1177
AasI GACNNNNNNGTC 1 cut(s) 319
AatII GACGTC 1 cut(s) 1239
Acc36I ACCTGC 1 cut(s) 1177
AccB1I GGYRCC 3 cut(s) 67, 96, 362
AccB7I CCANNNNNTGG 1 cut(s) 93
AccI GTMKAC 2 cut(s) 43, 195
AciI CCGC 3 cut(s) 166, 171, 279
AclWI GGATC 1 cut(s) 1157
AcsI RAATTY 2 cut(s) 1133, 1315
AcuI CTGAAG 2 cut(s) 559, 1050
AcyI GRCGYC 1 cut(s) 1236
AfaI GTAC 2 cut(s) 395, 1203
AfiI CCNNNNNNNGG 4 cut(s) 93, 151, 152, 762
AgsI TTSAA 5 cut(s) 697, 812, 947, 1222, 1337
AjnI CCWGG 3 cut(s) 258, 676, 761
AloI GAACNNNNNNTCC 2 cut(s) 636, 668
AluBI AGCT 4 cut(s) 614, 702, 709, 800
AluI AGCT 4 cut(s) 614, 702, 709, 800
Alw26I GTCTC 4 cut(s) 327, 881, 1017, 1243
AlwI GGATC 1 cut(s) 1157
AlwNI CAGNNNCTG 1 cut(s) 803
Ama87I CYCGRG 1 cut(s) 1014
ApeKI GCWGC 3 cut(s) 536, 664, 797
ApoI RAATTY 2 cut(s) 1133, 1315
ArsI GACNNNNNNTTYG 4 cut(s) 636, 668, 1307, 1339
Asp700I GAANNNNTTC 1 cut(s) 963
AspLEI GCGC 1 cut(s) 139
AsuC2I CCSGG 1 cut(s) 360
AsuHPI GGTGA 1 cut(s) 1276
AsuII TTCGAA 1 cut(s) 908
AvaI CYCGRG 1 cut(s) 1014
BanI GGYRCC 3 cut(s) 67, 96, 362
BbvI GCAGC 3 cut(s) 523, 651, 809
BccI CCATC 1 cut(s) 473
BciT130I CCWGG 3 cut(s) 260, 678, 763
BciVI GTATCC 2 cut(s) 992, 1118
BcnI CCSGG 1 cut(s) 360
BcoDI GTCTC 4 cut(s) 327, 881, 1017, 1243
BfaI CTAG 1 cut(s) 1366
BfuAI ACCTGC 1 cut(s) 1177
BfuI GTATCC 2 cut(s) 992, 1118
BglII AGATCT 1 cut(s) 1069
BisI GCNGC 3 cut(s) 537, 665, 798
BlsI GCNGC 3 cut(s) 538, 666, 799
Bme1390I CCNGG 4 cut(s) 260, 360, 678, 763
BmeT110I CYCGRG 1 cut(s) 1014
BmiI GGNNCC 6 cut(s) 69, 98, 364, 767, 844, 1044
BmrFI CCNGG 4 cut(s) 260, 360, 678, 763
BmsI GCATC 2 cut(s) 217, 611
Bpu14I TTCGAA 1 cut(s) 908
BpuMI CCSGG 1 cut(s) 360
BsaAI YACGTR 1 cut(s) 1190
BsaHI GRCGYC 1 cut(s) 1236
BsaJI CCNNGG 4 cut(s) 246, 676, 846, 1038
BsaWI WCCGGW 1 cut(s) 839
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 151, 152, 762
Bse1I ACTGG 1 cut(s) 1009
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 3 cut(s) 260, 678, 763
BseDI CCNNGG 4 cut(s) 246, 676, 846, 1038
BseGI GGATG 2 cut(s) 602, 857
BseLI CCNNNNNNNGG 4 cut(s) 93, 151, 152, 762
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 4 cut(s) 624, 678, 982, 1229
BseNI ACTGG 1 cut(s) 1009
BseXI GCAGC 3 cut(s) 523, 651, 809
BsgI GTGCAG 2 cut(s) 143, 733
BshNI GGYRCC 3 cut(s) 67, 96, 362
BsiHKCI CYCGRG 1 cut(s) 1014
BsiSI CCGG 2 cut(s) 359, 840
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 4 cut(s) 93, 151, 152, 762
BsmAI GTCTC 4 cut(s) 327, 881, 1017, 1243
BsmBI CGTCTC 1 cut(s) 1243
BsmFI GGGAC 1 cut(s) 127
BsmI GAATGC 1 cut(s) 900
BsoBI CYCGRG 1 cut(s) 1014
Bsp119I TTCGAA 1 cut(s) 908
Bsp143I GATC 5 cut(s) 340, 382, 1069, 1162, 1362
Bsp19I CCATGG 3 cut(s) 246, 846, 1038
BspACI CCGC 3 cut(s) 166, 171, 279
BspCNI CTCAG 4 cut(s) 623, 679, 981, 1228
BspLI GGNNCC 6 cut(s) 69, 98, 364, 767, 844, 1044
BspMI ACCTGC 1 cut(s) 1177
BspPI GGATC 1 cut(s) 1157
BspT104I TTCGAA 1 cut(s) 908
BspT107I GGYRCC 3 cut(s) 67, 96, 362
BsrDI GCAATG 1 cut(s) 415
BsrI ACTGG 1 cut(s) 1009
BssECI CCNNGG 4 cut(s) 246, 676, 846, 1038
BssMI GATC 5 cut(s) 340, 382, 1069, 1162, 1362
BssNI GRCGYC 1 cut(s) 1236
BssT1I CCWWGG 3 cut(s) 246, 846, 1038
Bst2UI CCWGG 3 cut(s) 260, 678, 763
Bst4CI ACNGT 3 cut(s) 193, 648, 793
BstACI GRCGYC 1 cut(s) 1236
BstAPI GCANNNNNTGC 1 cut(s) 803
BstBAI YACGTR 1 cut(s) 1190
BstBI TTCGAA 1 cut(s) 908
BstC8I GCNNGC 2 cut(s) 716, 1195
BstDEI CTNAG 4 cut(s) 610, 687, 968, 1215
BstDSI CCRYGG 3 cut(s) 246, 846, 1038
BstF5I GGATG 2 cut(s) 602, 857
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 5 cut(s) 343, 385, 1072, 1165, 1365
BstMAI GTCTC 4 cut(s) 327, 881, 1017, 1243
BstMBI GATC 5 cut(s) 340, 382, 1069, 1162, 1362
BstMWI GCNNNNNNNGC 4 cut(s) 542, 715, 739, 803
BstNI CCWGG 3 cut(s) 260, 678, 763
BstNSI RCATGY 1 cut(s) 1197
BstSCI CCNGG 4 cut(s) 258, 358, 676, 761
BstV1I GCAGC 3 cut(s) 523, 651, 809
BstX2I RGATCY 3 cut(s) 1069, 1162, 1362
BstXI CCANNNNNNTGG 1 cut(s) 1318
BstYI RGATCY 3 cut(s) 1069, 1162, 1362
BsuI GTATCC 2 cut(s) 992, 1118
BtgI CCRYGG 3 cut(s) 246, 846, 1038
BtsCI GGATG 2 cut(s) 602, 857
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 2 cut(s) 366, 378
BveI ACCTGC 1 cut(s) 1177
Cac8I GCNNGC 2 cut(s) 716, 1195
CaiI CAGNNNCTG 1 cut(s) 803
CfoI GCGC 1 cut(s) 139
Csp6I GTAC 2 cut(s) 394, 1202
CviAII CATG 8 cut(s) 72, 76, 242, 247, 515, 847, 1039, 1194
CviQI GTAC 2 cut(s) 394, 1202
DdeI CTNAG 4 cut(s) 610, 687, 968, 1215
DpnI GATC 5 cut(s) 342, 384, 1071, 1164, 1364
DpnII GATC 5 cut(s) 340, 382, 1069, 1162, 1362
DrdI GACNNNNNNGTC 1 cut(s) 319
DseDI GACNNNNNNGTC 1 cut(s) 319
Eco130I CCWWGG 3 cut(s) 246, 846, 1038
Eco57I CTGAAG 2 cut(s) 559, 1050
Eco88I CYCGRG 1 cut(s) 1014
EcoRII CCWGG 3 cut(s) 258, 676, 761
EcoT14I CCWWGG 3 cut(s) 246, 846, 1038
EcoT22I ATGCAT 2 cut(s) 77, 779
ErhI CCWWGG 3 cut(s) 246, 846, 1038
Esp3I CGTCTC 1 cut(s) 1243
FaeI CATG 8 cut(s) 75, 79, 245, 250, 518, 850, 1042, 1197
FalI AAGNNNNNCTT 2 cut(s) 1048, 1080
FaqI GGGAC 1 cut(s) 127
FatI CATG 8 cut(s) 71, 75, 241, 246, 514, 846, 1038, 1193
FblI GTMKAC 2 cut(s) 43, 195
Fnu4HI GCNGC 3 cut(s) 537, 665, 798
FokI GGATG 2 cut(s) 589, 864
Fsp4HI GCNGC 3 cut(s) 537, 665, 798
FspBI CTAG 1 cut(s) 1366
GlaI GCGC 1 cut(s) 138
GluI GCNGC 3 cut(s) 537, 665, 798
HapII CCGG 2 cut(s) 359, 840
HhaI GCGC 1 cut(s) 139
Hin1I GRCGYC 1 cut(s) 1236
Hin1II CATG 8 cut(s) 75, 79, 245, 250, 518, 850, 1042, 1197
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HpaII CCGG 2 cut(s) 359, 840
HphI GGTGA 1 cut(s) 1276
Hpy166II GTNNAC 5 cut(s) 44, 196, 505, 727, 1311
Hpy188I TCNGA 5 cut(s) 55, 559, 578, 971, 1069
Hpy188III TCNNGA 5 cut(s) 386, 631, 824, 1014, 1180
Hpy8I GTNNAC 5 cut(s) 44, 196, 505, 727, 1311
HpyAV CCTTC 3 cut(s) 52, 564, 904
HpyCH4III ACNGT 3 cut(s) 193, 648, 793
HpyCH4IV ACGT 2 cut(s) 1189, 1236
HpyF10VI GCNNNNNNNGC 4 cut(s) 542, 715, 739, 803
HpyF3I CTNAG 4 cut(s) 610, 687, 968, 1215
HpySE526I ACGT 2 cut(s) 1189, 1236
Hsp92I GRCGYC 1 cut(s) 1236
Hsp92II CATG 8 cut(s) 75, 79, 245, 250, 518, 850, 1042, 1197
HspAI GCGC 1 cut(s) 137
Kzo9I GATC 5 cut(s) 340, 382, 1069, 1162, 1362
LmnI GCTCC 2 cut(s) 707, 842
Lsp1109I GCAGC 3 cut(s) 523, 651, 809
LweI GCATC 2 cut(s) 217, 611
MaeI CTAG 1 cut(s) 1366
MaeII ACGT 2 cut(s) 1189, 1236
MalI GATC 5 cut(s) 342, 384, 1071, 1164, 1364
MboI GATC 5 cut(s) 340, 382, 1069, 1162, 1362
MboII GAAGA 5 cut(s) 154, 263, 311, 839, 959
MflI RGATCY 3 cut(s) 1069, 1162, 1362
MluCI AATT 8 cut(s) 217, 235, 287, 588, 669, 904, 1133, 1315
MlyI GAGTC 2 cut(s) 1269, 1331
MmeI TCCRAC 1 cut(s) 1085
MnlI CCTC 8 cut(s) 10, 156, 275, 682, 728, 794, 1010, 1221
Mph1103I ATGCAT 2 cut(s) 77, 779
MroXI GAANNNNTTC 1 cut(s) 963
MseI TTAA 2 cut(s) 425, 1116
MslI CAYNNNNRTG 2 cut(s) 80, 531
MspA1I CMGCKG 1 cut(s) 614
MspI CCGG 2 cut(s) 359, 840
MspR9I CCNGG 4 cut(s) 260, 360, 678, 763
Mva1269I GAATGC 1 cut(s) 900
MvaI CCWGG 3 cut(s) 260, 678, 763
MwoI GCNNNNNNNGC 4 cut(s) 542, 715, 739, 803
NciI CCSGG 1 cut(s) 360
NcoI CCATGG 3 cut(s) 246, 846, 1038
NdeII GATC 5 cut(s) 340, 382, 1069, 1162, 1362
NlaIII CATG 8 cut(s) 75, 79, 245, 250, 518, 850, 1042, 1197
NlaIV GGNNCC 6 cut(s) 69, 98, 364, 767, 844, 1044
NsiI ATGCAT 2 cut(s) 77, 779
NspI RCATGY 1 cut(s) 1197
NspV TTCGAA 1 cut(s) 908
PaeI GCATGC 1 cut(s) 1197
PaeR7I CTCGAG 1 cut(s) 1014
PaqCI CACCTGC 1 cut(s) 1177
PcsI WCGNNNNNNNCGW 1 cut(s) 311
PctI GAATGC 1 cut(s) 900
PdmI GAANNNNTTC 1 cut(s) 963
PfeI GAWTC 9 cut(s) 302, 374, 560, 693, 950, 964, 1005, 1034, 1267
PflMI CCANNNNNTGG 1 cut(s) 93
PfoI TCCNGGA 1 cut(s) 761
PkrI GCNGC 3 cut(s) 538, 666, 799
PleI GAGTC 2 cut(s) 1269, 1330
PpsI GAGTC 2 cut(s) 1269, 1330
Ppu21I YACGTR 1 cut(s) 1190
PsiI TTATAA 1 cut(s) 1329
Psp6I CCWGG 3 cut(s) 258, 676, 761
PspGI CCWGG 3 cut(s) 258, 676, 761
PspN4I GGNNCC 6 cut(s) 69, 98, 364, 767, 844, 1044
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 3 cut(s) 1069, 1162, 1362
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 2 cut(s) 395, 1203
RsaNI GTAC 2 cut(s) 394, 1202
RseI CAYNNNNRTG 2 cut(s) 80, 531
SaqAI TTAA 2 cut(s) 425, 1116
SatI GCNGC 3 cut(s) 537, 665, 798
Sau3AI GATC 5 cut(s) 340, 382, 1069, 1162, 1362
SchI GAGTC 2 cut(s) 1269, 1331
ScrFI CCNGG 4 cut(s) 260, 360, 678, 763
SfaNI GCATC 2 cut(s) 217, 611
Sfr274I CTCGAG 1 cut(s) 1014
SfuI TTCGAA 1 cut(s) 908
SlaI CTCGAG 1 cut(s) 1014
SmiMI CAYNNNNRTG 2 cut(s) 80, 531
SmlI CTYRAG 1 cut(s) 1014
SmoI CTYRAG 1 cut(s) 1014
SphI GCATGC 1 cut(s) 1197
Sse9I AATT 8 cut(s) 217, 235, 287, 588, 669, 904, 1133, 1315
SsiI CCGC 3 cut(s) 166, 171, 279
SspMI CTAG 1 cut(s) 1366
StyD4I CCNGG 4 cut(s) 258, 358, 676, 761
StyI CCWWGG 3 cut(s) 246, 846, 1038
TaaI ACNGT 3 cut(s) 193, 648, 793
TaiI ACGT 2 cut(s) 1192, 1239
TaqI TCGA 6 cut(s) 343, 655, 866, 900, 908, 1015
TasI AATT 8 cut(s) 217, 235, 287, 588, 669, 904, 1133, 1315
TfiI GAWTC 9 cut(s) 302, 374, 560, 693, 950, 964, 1005, 1034, 1267
Tru1I TTAA 2 cut(s) 425, 1116
Tru9I TTAA 2 cut(s) 425, 1116
TscAI CASTG 2 cut(s) 373, 385
TseI GCWGC 3 cut(s) 536, 664, 797
TspDTI ATGAA 6 cut(s) 230, 443, 846, 884, 902, 1145
TspGWI ACGGA 1 cut(s) 1017
TspRI CASTG 2 cut(s) 373, 385
Van91I CCANNNNNTGG 1 cut(s) 93
XapI RAATTY 2 cut(s) 1133, 1315
XceI RCATGY 1 cut(s) 1197
XhoI CTCGAG 1 cut(s) 1014
XmiI GTMKAC 2 cut(s) 43, 195
XmnI GAANNNNTTC 1 cut(s) 963
XspI CTAG 1 cut(s) 1366
ZraI GACGTC 1 cut(s) 1237
Zsp2I ATGCAT 2 cut(s) 77, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.