Rh2CG163300

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000947
Physical Location & Seq
Forward (+)
14443528 .. 14459036
15509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG163300.1

Sequence Viewer

Length: 1722 bp
ATGGGGGATTTATGCGAGGATTTGTGGGTTGAGATTTTGGGTAGACTATCACCGGAGAAGGACTTGGTGCCATGCATGAGCGTGTCCAGGTTATGGCACCGAATAACATCTGGTCTTTGGGTGCAGAGATTTTGGGCGCAGTCCCCTCCTTTGGGTCTTTTCTTCCGCACCGCCACCACCAGCACCACCACCGTAGACAAGGTATTGCATCGTATGAATTATATCTCTTTATATAATTATCATGCCATGGAAGATAACCTGCAAAGCACTCGTTCTTGCGGAGGTCAATTTTATGACGAAGAATCGTATAGGTCGAAAAAGTCTCATTGGCAGTATTTTGATCGACAATGGTATAAGCCGGGTGCCACTGCTGAATCCACTGATCGTGATGAGTACCTAAATGGTTGCAATGGTTTAGTTTTGTTAATGAAATCAGCAACTTATCAGTTTTATGTTTGCAATCCCATCACAAGACAACAAGTTGCCATACCTAAAGCCTGTGTCCACCAAAACCATGAAGATCAGCATTTTTGTGCTGCTTTGGCTTTTGACCCTTCCGAATCTGCTCGTCACTTCAGAGTTGTTAGAATTGATTATTCTGCACCCTACTCAGCTGTGCTGGATATATTCTCGTCTGAATATGGACTGTGGGTTCGACATTGGCTGCAAATTGACCCAGGTTTTACTGAGGGATTCAAAAGCTCCAAGTTGTGCAGGCATTTTGTTTATTTCCGGGGCAAGCTATACAGCATAGCCCTATCCTGGAACCTTTTATGCATTGACCTCAATACTGTTGCAGCTTTTGCCCTTGAACTACCTGTTCTAGGGAATGAAAAGACCGGAGCCATGGGATGTCTTGGGGTGTCGATGAATAGTCTCTGCTATATGAAGCGAATGCTCGATAATTCGAAGGACTTTCACAATCTGGCAGTTTGGTATAATGATGATGAATCTGGGGAATGGATTCTCAGATATAGTGTTTCTTGTCGCATATTGGGATACGGATTCCAGTCTTTAGGTTATGACTACGATGATTCCATGGAACCCTGTGCAATAAGTCCAACTTCAGATCTCTTGTTCTTTGGCAATCTCAACTTGATTTGTTGCTTCGTACCTTTCTACACTCAGTTGAACAAGAGGAATGACGTCTCCACTCCATTAGCAACAGCTTGTGAGCGCACAGAAATTTTAAGTGTGGAAGTACCATATGGGAAACAAGAAGATGGTGAATCAGTTGAAACGCAATCAAAGCTGAAGATTATTCTTCGTATCGCGTCTTCTTCTACTACAGACTTCTCCGGTTCTCTCAAAGGAAACCAGCTGGAGCCTCTTCCTTCGCATCTGATGATGGGTCAGATTAGGTGTCCGATAACAGGAATTGTCATCAATACATATTTGGCACCACCTGGTGATAACCAGGGAGGATATGCAACCGTAGTACAATGCACAAACACGTTTGATGAGCAGGACTATGCCATCAAGAAAATTCCCATTGGAGAAGTGATTGAAGATAATCCCGAGTCACAAGCTTTCAGCTATGCATCTCTTTCTTGGACTGAAAACAAGTACTGCGGTTTGCTTGATGAAGATTGGGATGGGGACCCAATAGAAACAGAAAATCCATGCTTGTACATTCAATTGGAACTATGTGCCAGTTCCTTGGAACACAAGCTGCAGCCAAATGAACAATGCATGAACAATTGGACCACCAGAGAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

573

Amino Acids

65.22

Weight (kDa)

5.01

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 6 - 42 7.5e-06 F-box-like
Beta-prop_KIB1-4 PF03478 128 - 267 4.5e-08 KIB1-4 beta-propeller
b-prop_At3g26010-like PF24750 130 - 333 8.6e-12 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 135 - 312 2.5e-07 F-box associated beta propeller domain
Beta-prop_AT5G49610-like PF23635 136 - 330 3.2e-06 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1149
Acc36I ACCTGC 1 cut(s) 267
AccB1I GGYRCC 4 cut(s) 67, 96, 362, 1399
AccB7I CCANNNNNTGG 1 cut(s) 93
AccI GTMKAC 2 cut(s) 43, 195
AccII CGCG 1 cut(s) 1274
AciI CCGC 4 cut(s) 166, 171, 279, 1572
AcsI RAATTY 2 cut(s) 1185, 1485
AcuI CTGAAG 3 cut(s) 559, 1050, 1274
AcyI GRCGYC 1 cut(s) 1146
AdeI CACNNNGTG 1 cut(s) 1409
AfaI GTAC 6 cut(s) 395, 1113, 1203, 1440, 1568, 1631
AfiI CCNNNNNNNGG 6 cut(s) 93, 151, 152, 762, 824, 1373
AflIII ACRYGT 1 cut(s) 1452
AgsI TTSAA 6 cut(s) 697, 812, 1132, 1238, 1508, 1637
AjnI CCWGG 5 cut(s) 86, 676, 761, 1405, 1416
AloI GAACNNNNNNTCC 2 cut(s) 636, 668
Alw26I GTCTC 3 cut(s) 327, 881, 1153
Ama87I CYCGRG 1 cut(s) 1517
ApeKI GCWGC 5 cut(s) 536, 664, 797, 1672, 1675
ApoI RAATTY 2 cut(s) 1185, 1485
ArsI GACNNNNNNTTYG 2 cut(s) 636, 668
Asp700I GAANNNNTTC 1 cut(s) 963
AspLEI GCGC 2 cut(s) 139, 1179
AspS9I GGNCC 2 cut(s) 1600, 1704
AsuC2I CCSGG 2 cut(s) 360, 734
AsuHPI GGTGA 3 cut(s) 42, 1238, 1421
AsuII TTCGAA 1 cut(s) 908
AvaI CYCGRG 1 cut(s) 1517
AvaII GGWCC 2 cut(s) 1600, 1704
BanI GGYRCC 4 cut(s) 67, 96, 362, 1399
BarI GAAGNNNNNNTAC 2 cut(s) 1261, 1293
BbsI GAAGAC 1 cut(s) 1269
BbvI GCAGC 5 cut(s) 523, 651, 809, 1659, 1687
BccI CCATC 5 cut(s) 473, 1217, 1342, 1484, 1589
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 5 cut(s) 88, 678, 763, 1407, 1418
BciVI GTATCC 1 cut(s) 992
BcnI CCSGG 2 cut(s) 360, 734
BcoDI GTCTC 3 cut(s) 327, 881, 1153
BfaI CTAG 1 cut(s) 824
BfmI CTRYAG 2 cut(s) 1287, 1673
BfuAI ACCTGC 1 cut(s) 267
BfuI GTATCC 1 cut(s) 992
BglII AGATCT 1 cut(s) 1069
BisI GCNGC 5 cut(s) 537, 665, 798, 1673, 1676
BlsI GCNGC 5 cut(s) 538, 666, 799, 1674, 1677
BmcAI AGTACT 1 cut(s) 1568
Bme1390I CCNGG 7 cut(s) 88, 360, 678, 734, 763, 1407, 1418
Bme18I GGWCC 2 cut(s) 1600, 1704
BmeT110I CYCGRG 1 cut(s) 1517
BmgT120I GGNCC 2 cut(s) 1600, 1704
BmrFI CCNGG 7 cut(s) 88, 360, 678, 734, 763, 1407, 1418
BmsI GCATC 3 cut(s) 217, 1348, 1550
BpiI GAAGAC 1 cut(s) 1269
BpmI CTGGAG 1 cut(s) 1343
Bpu14I TTCGAA 1 cut(s) 908
BpuMI CCSGG 2 cut(s) 360, 734
BsaHI GRCGYC 1 cut(s) 1146
BsaJI CCNNGG 7 cut(s) 246, 676, 733, 846, 1038, 1417, 1659
BsaWI WCCGGW 3 cut(s) 52, 839, 1298
Bsc4I CCNNNNNNNGG 6 cut(s) 93, 151, 152, 762, 824, 1373
Bse1I ACTGG 2 cut(s) 1009, 1653
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 5 cut(s) 88, 678, 763, 1407, 1418
BseDI CCNNGG 7 cut(s) 246, 676, 733, 846, 1038, 1417, 1659
BseGI GGATG 2 cut(s) 857, 1600
BseLI CCNNNNNNNGG 6 cut(s) 93, 151, 152, 762, 824, 1373
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 4 cut(s) 624, 678, 982, 1139
BseNI ACTGG 2 cut(s) 1009, 1653
BseXI GCAGC 5 cut(s) 523, 651, 809, 1659, 1687
BsgI GTGCAG 3 cut(s) 143, 585, 733
Bsh1236I CGCG 1 cut(s) 1274
BshNI GGYRCC 4 cut(s) 67, 96, 362, 1399
BsiHKCI CYCGRG 1 cut(s) 1517
BsiSI CCGG 5 cut(s) 53, 359, 733, 840, 1299
BslFI GGGAC 2 cut(s) 127, 1613
BslI CCNNNNNNNGG 6 cut(s) 93, 151, 152, 762, 824, 1373
BsmAI GTCTC 3 cut(s) 327, 881, 1153
BsmBI CGTCTC 1 cut(s) 1153
BsmFI GGGAC 2 cut(s) 127, 1613
BsmI GAATGC 1 cut(s) 900
BsoBI CYCGRG 1 cut(s) 1517
Bsp119I TTCGAA 1 cut(s) 908
Bsp1407I TGTACA 1 cut(s) 1629
Bsp143I GATC 4 cut(s) 340, 382, 520, 1069
Bsp19I CCATGG 3 cut(s) 246, 846, 1038
BspACI CCGC 4 cut(s) 166, 171, 279, 1572
BspCNI CTCAG 4 cut(s) 623, 679, 981, 1138
BspFNI CGCG 1 cut(s) 1274
BspMAI CTGCAG 1 cut(s) 1677
BspMI ACCTGC 1 cut(s) 267
BspT104I TTCGAA 1 cut(s) 908
BspT107I GGYRCC 4 cut(s) 67, 96, 362, 1399
BsrDI GCAATG 1 cut(s) 415
BsrGI TGTACA 1 cut(s) 1629
BsrI ACTGG 2 cut(s) 1009, 1653
BssECI CCNNGG 7 cut(s) 246, 676, 733, 846, 1038, 1417, 1659
BssMI GATC 4 cut(s) 340, 382, 520, 1069
BssNI GRCGYC 1 cut(s) 1146
BssT1I CCWWGG 4 cut(s) 246, 846, 1038, 1659
Bst2UI CCWGG 5 cut(s) 88, 678, 763, 1407, 1418
Bst4CI ACNGT 4 cut(s) 193, 648, 793, 1435
Bst6I CTCTTC 1 cut(s) 1335
BstACI GRCGYC 1 cut(s) 1146
BstAPI GCANNNNNTGC 1 cut(s) 803
BstAUI TGTACA 1 cut(s) 1629
BstBI TTCGAA 1 cut(s) 908
BstC8I GCNNGC 2 cut(s) 716, 740
BstDEI CTNAG 4 cut(s) 610, 687, 968, 1125
BstDSI CCRYGG 3 cut(s) 246, 846, 1038
BstENI CCTNNNNNAGG 1 cut(s) 822
BstF5I GGATG 2 cut(s) 857, 1600
BstFNI CGCG 1 cut(s) 1274
BstHHI GCGC 2 cut(s) 139, 1179
BstKTI GATC 4 cut(s) 343, 385, 523, 1072
BstMAI GTCTC 3 cut(s) 327, 881, 1153
BstMBI GATC 4 cut(s) 340, 382, 520, 1069
BstMWI GCNNNNNNNGC 3 cut(s) 542, 803, 1249
BstNI CCWGG 5 cut(s) 88, 678, 763, 1407, 1418
BstSCI CCNGG 7 cut(s) 86, 358, 676, 732, 761, 1405, 1416
BstSFI CTRYAG 2 cut(s) 1287, 1673
BstUI CGCG 1 cut(s) 1274
BstV1I GCAGC 5 cut(s) 523, 651, 809, 1659, 1687
BstV2I GAAGAC 1 cut(s) 1269
BstX2I RGATCY 1 cut(s) 1069
BstXI CCANNNNNNTGG 1 cut(s) 1660
BstYI RGATCY 1 cut(s) 1069
BsuI GTATCC 1 cut(s) 992
BtgI CCRYGG 3 cut(s) 246, 846, 1038
BtsCI GGATG 2 cut(s) 857, 1600
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 2 cut(s) 366, 378
BveI ACCTGC 1 cut(s) 267
Cac8I GCNNGC 2 cut(s) 716, 740
CfoI GCGC 2 cut(s) 139, 1179
Cfr13I GGNCC 2 cut(s) 1600, 1704
CseI GACGC 1 cut(s) 1263
CsiI ACCWGGT 1 cut(s) 1405
Csp6I GTAC 6 cut(s) 394, 1112, 1202, 1439, 1567, 1630
CviAII CATG 9 cut(s) 72, 76, 242, 247, 515, 847, 1039, 1623, 1693
CviQI GTAC 6 cut(s) 394, 1112, 1202, 1439, 1567, 1630
DdeI CTNAG 4 cut(s) 610, 687, 968, 1125
DpnI GATC 4 cut(s) 342, 384, 522, 1071
DpnII GATC 4 cut(s) 340, 382, 520, 1069
DraIII CACNNNGTG 1 cut(s) 1409
Eam1104I CTCTTC 1 cut(s) 1335
EarI CTCTTC 1 cut(s) 1335
Eco130I CCWWGG 4 cut(s) 246, 846, 1038, 1659
Eco47I GGWCC 2 cut(s) 1600, 1704
Eco57I CTGAAG 3 cut(s) 559, 1050, 1274
Eco88I CYCGRG 1 cut(s) 1517
EcoNI CCTNNNNNAGG 1 cut(s) 822
EcoO109I RGGNCCY 1 cut(s) 1600
EcoRII CCWGG 5 cut(s) 86, 676, 761, 1405, 1416
EcoT14I CCWWGG 4 cut(s) 246, 846, 1038, 1659
EcoT22I ATGCAT 4 cut(s) 77, 779, 1543, 1694
ErhI CCWWGG 4 cut(s) 246, 846, 1038, 1659
Esp3I CGTCTC 1 cut(s) 1153
FaeI CATG 9 cut(s) 75, 79, 245, 250, 518, 850, 1042, 1626, 1696
FalI AAGNNNNNCTT 2 cut(s) 1048, 1080
FaqI GGGAC 2 cut(s) 127, 1613
FatI CATG 9 cut(s) 71, 75, 241, 246, 514, 846, 1038, 1622, 1692
FauNDI CATATG 1 cut(s) 1207
FblI GTMKAC 2 cut(s) 43, 195
Fnu4HI GCNGC 5 cut(s) 537, 665, 798, 1673, 1676
FokI GGATG 2 cut(s) 864, 1607
Fsp4HI GCNGC 5 cut(s) 537, 665, 798, 1673, 1676
FspBI CTAG 1 cut(s) 824
GlaI GCGC 2 cut(s) 138, 1178
GluI GCNGC 5 cut(s) 537, 665, 798, 1673, 1676
GsuI CTGGAG 1 cut(s) 1343
HapII CCGG 5 cut(s) 53, 359, 733, 840, 1299
HgaI GACGC 1 cut(s) 1263
HhaI GCGC 2 cut(s) 139, 1179
Hin1I GRCGYC 1 cut(s) 1146
Hin1II CATG 9 cut(s) 75, 79, 245, 250, 518, 850, 1042, 1626, 1696
Hin6I GCGC 2 cut(s) 137, 1177
HinP1I GCGC 2 cut(s) 137, 1177
HindIII AAGCTT 1 cut(s) 1527
HpaII CCGG 5 cut(s) 53, 359, 733, 840, 1299
HphI GGTGA 3 cut(s) 42, 1238, 1421
Hpy166II GTNNAC 3 cut(s) 44, 196, 505
Hpy188I TCNGA 8 cut(s) 559, 578, 637, 971, 1069, 1344, 1356, 1368
Hpy188III TCNNGA 3 cut(s) 386, 1480, 1517
Hpy8I GTNNAC 3 cut(s) 44, 196, 505
HpyAV CCTTC 4 cut(s) 52, 564, 904, 1344
HpyCH4III ACNGT 4 cut(s) 193, 648, 793, 1435
HpyCH4IV ACGT 2 cut(s) 1146, 1454
HpyF10VI GCNNNNNNNGC 3 cut(s) 542, 803, 1249
HpyF3I CTNAG 4 cut(s) 610, 687, 968, 1125
HpySE526I ACGT 2 cut(s) 1146, 1454
Hsp92I GRCGYC 1 cut(s) 1146
Hsp92II CATG 9 cut(s) 75, 79, 245, 250, 518, 850, 1042, 1626, 1696
HspAI GCGC 2 cut(s) 137, 1177
KflI GGGWCCC 1 cut(s) 1600
Kzo9I GATC 4 cut(s) 340, 382, 520, 1069
LmnI GCTCC 3 cut(s) 707, 842, 1324
Lsp1109I GCAGC 5 cut(s) 523, 651, 809, 1659, 1687
LweI GCATC 3 cut(s) 217, 1348, 1550
MabI ACCWGGT 1 cut(s) 1405
MaeI CTAG 1 cut(s) 824
MaeII ACGT 2 cut(s) 1146, 1454
MaeIII GTNAC 2 cut(s) 569, 1521
MalI GATC 4 cut(s) 342, 384, 522, 1071
MboI GATC 4 cut(s) 340, 382, 520, 1069
MfeI CAATTG 2 cut(s) 1637, 1699
MflI RGATCY 1 cut(s) 1069
MlyI GAGTC 1 cut(s) 1529
MmeI TCCRAC 1 cut(s) 1085
MnlI CCTC 8 cut(s) 10, 156, 275, 682, 794, 1131, 1338, 1415
Mph1103I ATGCAT 4 cut(s) 77, 779, 1543, 1694
MroXI GAANNNNTTC 1 cut(s) 963
MseI TTAA 2 cut(s) 425, 1190
MslI CAYNNNNRTG 2 cut(s) 80, 531
MspA1I CMGCKG 2 cut(s) 614, 1321
MspI CCGG 5 cut(s) 53, 359, 733, 840, 1299
MspR9I CCNGG 7 cut(s) 88, 360, 678, 734, 763, 1407, 1418
MunI CAATTG 2 cut(s) 1637, 1699
Mva1269I GAATGC 1 cut(s) 900
MvaI CCWGG 5 cut(s) 88, 678, 763, 1407, 1418
MvnI CGCG 1 cut(s) 1274
MwoI GCNNNNNNNGC 3 cut(s) 542, 803, 1249
NciI CCSGG 2 cut(s) 360, 734
NcoI CCATGG 3 cut(s) 246, 846, 1038
NdeI CATATG 1 cut(s) 1207
NdeII GATC 4 cut(s) 340, 382, 520, 1069
NlaIII CATG 9 cut(s) 75, 79, 245, 250, 518, 850, 1042, 1626, 1696
NmuCI GTSAC 2 cut(s) 569, 1521
NsiI ATGCAT 4 cut(s) 77, 779, 1543, 1694
NspV TTCGAA 1 cut(s) 908
PcsI WCGNNNNNNNCGW 1 cut(s) 311
PctI GAATGC 1 cut(s) 900
PdmI GAANNNNTTC 1 cut(s) 963
PfeI GAWTC 9 cut(s) 302, 374, 560, 693, 950, 964, 1005, 1034, 1229
PflMI CCANNNNNTGG 1 cut(s) 93
PfoI TCCNGGA 1 cut(s) 761
PkrI GCNGC 5 cut(s) 538, 666, 799, 1674, 1677
PleI GAGTC 1 cut(s) 1528
PpsI GAGTC 1 cut(s) 1528
PpuMI RGGWCCY 1 cut(s) 1600
Psp5II RGGWCCY 1 cut(s) 1600
Psp6I CCWGG 5 cut(s) 86, 676, 761, 1405, 1416
PspGI CCWGG 5 cut(s) 86, 676, 761, 1405, 1416
PspPI GGNCC 2 cut(s) 1600, 1704
PspPPI RGGWCCY 1 cut(s) 1600
PstI CTGCAG 1 cut(s) 1677
PsuI RGATCY 1 cut(s) 1069
PvuII CAGCTG 2 cut(s) 614, 1321
RsaI GTAC 6 cut(s) 395, 1113, 1203, 1440, 1568, 1631
RsaNI GTAC 6 cut(s) 394, 1112, 1202, 1439, 1567, 1630
RseI CAYNNNNRTG 2 cut(s) 80, 531
SaqAI TTAA 2 cut(s) 425, 1190
SatI GCNGC 5 cut(s) 537, 665, 798, 1673, 1676
Sau3AI GATC 4 cut(s) 340, 382, 520, 1069
Sau96I GGNCC 2 cut(s) 1600, 1704
ScaI AGTACT 1 cut(s) 1568
SchI GAGTC 1 cut(s) 1529
ScrFI CCNGG 7 cut(s) 88, 360, 678, 734, 763, 1407, 1418
SexAI ACCWGGT 1 cut(s) 1405
SfaNI GCATC 3 cut(s) 217, 1348, 1550
SfcI CTRYAG 2 cut(s) 1287, 1673
SfuI TTCGAA 1 cut(s) 908
SinI GGWCC 2 cut(s) 1600, 1704
SmiMI CAYNNNNRTG 2 cut(s) 80, 531
SsiI CCGC 4 cut(s) 166, 171, 279, 1572
SspMI CTAG 1 cut(s) 824
StyD4I CCNGG 7 cut(s) 86, 358, 676, 732, 761, 1405, 1416
StyI CCWWGG 4 cut(s) 246, 846, 1038, 1659
TaaI ACNGT 4 cut(s) 193, 648, 793, 1435
TaiI ACGT 2 cut(s) 1149, 1457
TaqI TCGA 6 cut(s) 314, 343, 655, 866, 900, 908
TatI WGTACW 3 cut(s) 1438, 1566, 1629
TfiI GAWTC 9 cut(s) 302, 374, 560, 693, 950, 964, 1005, 1034, 1229
Tru1I TTAA 2 cut(s) 425, 1190
Tru9I TTAA 2 cut(s) 425, 1190
TscAI CASTG 2 cut(s) 373, 385
TseFI GTSAC 2 cut(s) 569, 1521
TseI GCWGC 5 cut(s) 536, 664, 797, 1672, 1675
Tsp45I GTSAC 2 cut(s) 569, 1521
TspGWI ACGGA 1 cut(s) 1017
TspRI CASTG 2 cut(s) 373, 385
Van91I CCANNNNNTGG 1 cut(s) 93
VpaK11BI GGWCC 2 cut(s) 1600, 1704
XagI CCTNNNNNAGG 1 cut(s) 822
XapI RAATTY 2 cut(s) 1185, 1485
XmiI GTMKAC 2 cut(s) 43, 195
XmnI GAANNNNTTC 1 cut(s) 963
XspI CTAG 1 cut(s) 824
ZraI GACGTC 1 cut(s) 1147
ZrmI AGTACT 1 cut(s) 1568
Zsp2I ATGCAT 4 cut(s) 77, 779, 1543, 1694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.