Rw2G012350

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000947
Physical Location & Seq
Forward (+)
12863683 .. 12865431
1749 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G012350.1

Sequence Viewer

Length: 1512 bp
ATGGGGAATTTATGTGAAAATTTGTGGATTGAGATATTGGGTAGGTTAACCTCGGAGAAGGACTTGGTGCCATGCATGAGTGTGTCCAAGTTATGGCACCGAATCACATCGGATCTTTGGGTGAGAAAATTTTGGGCGCAGTCCCCTCCTTTGGGTCTTTTCTTGCGCACCGCCGCCAACACCACCGTGGGCAATCTAAAATATCATTGCATGAATTATATCTCTTTATATAATTATCATGACATAGAAGATAACCTGGAAAGCACCCGCGTTTTCGTTTTCGGACAACCATATTATGACATAGAAGATTATAGGGACCAAAAGATGATTTTGCAGACTTTTGGTAGAGAATGGCATTATCCGGGTTATTATGATGACGACGAGGATGATGGTGTTCTTGATGATGATTATAAACCCGCTGATCGTGATCAGTACCTAAACGGTTGTAATGGGTTACTTTTGTTATTGAAATCAGCAACTCATCAATTTTATGTTTGCAATCCCATCACAAGACAACAAGTCGCCATACCTAAAGCCCGTGTGCACAAAAACCATGATGATCAGCATTTTTGTGCTGCCTTGGCTTTTGACCCTTCTGAATCTCCTCATCACTACAGAATTGTGAGAATTGATTTTTCTCCGGAACCGGCTTCATCAGCCTCAGACTCCTCATCCTTGATAGACATATTTTCGTCCGAATATGGACAATGGATTCGACATAGGTTGCAGCTTGATTCAAGTCTCACTGACGGGTTGAAAGAGTGCAAGTTGTGCAGGCAATTTTTTTATTTGCGGGGAAAGTTATACAGTATAACCATGTCGTGGAACCTCCTATGCATTGACCTCAAGACTGTTGAAGCTTCTGCCCTTGAACTTCCTATCCCGGTGGCGGATAGAACCGGAGCCATGGGATGTCTTGGGGTGTCAAGGGACCTTCTTTGCTATATGAAGCGTATGCCTGATAAATCAAGTCGGTTTCACAATCTGGTAGTTTGGTCTTATGAAAAAGGTGAGTGGATTCCCCGATATAGTGTAAGTTGTCATTTGTTGGGAGAAGGAATATTGGATGATCTAGGCTATGACTACGATGACACCATGGAACCCTATGCAATAAGCCCAAGTTCGGATCTCTTGTTCTTTGGCACTCCAACCTTGATTTACTATACACCTGCTTGTTTCTTGACATTACGTGCTTGCTTAGTACCTTTCCACACTCAATTGCGTAAGGGGAACCACGTCTCCATTTCATTTTCTGAGGGCACAAACATTTTGAGTGTAGAAGAACCAAATGACAAACAAGAAGATGCTGAATCAGTGAAAGTAATAGATGTGAAGTTATATGATAGTATGAAAAAAGATCCATCACCGTCATTGGTTTATCCCAGTGAAATCATGGAAATGCATCTAGAGCTTGAGCAAATCCATGACGACACCTCTAGTGATGATGAACATGTCGTAGATCAGCTTGAAGGTCTTGTAGGGAGTATGAGGAAGAGGAGGAAAATCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

503

Amino Acids

57.93

Weight (kDa)

5.12

Isoelectric Point (pI)

57.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 8 - 43 5.3e-06 F-box-like
Beta-prop_KIB1-4 PF03478 140 - 295 2.2e-06 KIB1-4 beta-propeller
FBA_1 PF07734 144 - 320 1.3e-09 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 145 - 338 1.5e-10 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 411
AarI CACCTGC 1 cut(s) 1177
Acc16I TGCGCA 1 cut(s) 167
Acc36I ACCTGC 1 cut(s) 1177
AccB1I GGYRCC 2 cut(s) 67, 96
AccB7I CCANNNNNTGG 2 cut(s) 93, 822
AccII CGCG 1 cut(s) 270
AccIII TCCGGA 1 cut(s) 640
AciI CCGC 6 cut(s) 171, 174, 268, 417, 793, 890
AclWI GGATC 3 cut(s) 120, 1134, 1352
AcsI RAATTY 3 cut(s) 7, 19, 128
AfaI GTAC 2 cut(s) 434, 1203
AfiI CCNNNNNNNGG 6 cut(s) 93, 151, 152, 822, 889, 1123
AflIII ACRYGT 1 cut(s) 1450
AgsI TTSAA 6 cut(s) 469, 738, 757, 857, 872, 1469
AhdI GACNNNNNGTC 1 cut(s) 518
AjiI CACGTC 1 cut(s) 1237
AjnI CCWGG 1 cut(s) 255
AleI CACNNNNGTG 1 cut(s) 185
AloI GAACNNNNNNTCC 6 cut(s) 864, 896, 1118, 1150, 1223, 1255
AluBI AGCT 4 cut(s) 730, 860, 1411, 1465
AluI AGCT 4 cut(s) 730, 860, 1411, 1465
Alw21I GWGCWC 1 cut(s) 546
Alw26I GTCTC 2 cut(s) 746, 1243
Alw44I GTGCAC 1 cut(s) 542
AlwI GGATC 3 cut(s) 120, 1134, 1352
Aor13HI TCCGGA 1 cut(s) 640
ApaLI GTGCAC 1 cut(s) 542
ApeKI GCWGC 2 cut(s) 575, 727
ApoI RAATTY 3 cut(s) 7, 19, 128
ArsI GACNNNNNNTTYG 2 cut(s) 696, 728
AspLEI GCGC 2 cut(s) 139, 168
AspS9I GGNCC 2 cut(s) 316, 931
AsuC2I CCSGG 2 cut(s) 363, 884
AsuHPI GGTGA 3 cut(s) 133, 1022, 1356
AvaII GGWCC 2 cut(s) 316, 931
BaeGI GKGCMC 2 cut(s) 546, 1262
BanI GGYRCC 2 cut(s) 67, 96
Bbv12I GWGCWC 1 cut(s) 546
BbvI GCAGC 2 cut(s) 562, 739
BccI CCATC 3 cut(s) 383, 512, 1369
BciT130I CCWGG 1 cut(s) 257
BclI TGATCA 2 cut(s) 427, 559
BcnI CCSGG 2 cut(s) 363, 884
BcoDI GTCTC 2 cut(s) 746, 1243
BfaI CTAG 3 cut(s) 1073, 1406, 1437
BfmI CTRYAG 1 cut(s) 613
BfuAI ACCTGC 1 cut(s) 1177
BisI GCNGC 3 cut(s) 174, 576, 728
BlsI GCNGC 3 cut(s) 175, 577, 729
Bme1390I CCNGG 3 cut(s) 257, 363, 884
Bme18I GGWCC 2 cut(s) 316, 931
BmeRI GACNNNNNGTC 1 cut(s) 518
BmgBI CACGTC 1 cut(s) 1237
BmgT120I GGNCC 2 cut(s) 316, 931
BmiI GGNNCC 9 cut(s) 69, 98, 317, 645, 827, 904, 932, 1101, 1232
BmrFI CCNGG 3 cut(s) 257, 363, 884
BmrI ACTGGG 1 cut(s) 1377
BmsI GCATC 2 cut(s) 1294, 1411
BmuI ACTGGG 1 cut(s) 1377
BpuEI CTTGAG 2 cut(s) 830, 1433
BpuMI CCSGG 2 cut(s) 363, 884
BsaAI YACGTR 1 cut(s) 1190
BsaBI GATNNNNATC 1 cut(s) 426
BsaJI CCNNGG 5 cut(s) 51, 186, 579, 906, 1095
BsaWI WCCGGW 2 cut(s) 640, 899
BsaXI ACNNNNNCTCC 2 cut(s) 1223, 1253
Bsc4I CCNNNNNNNGG 6 cut(s) 93, 151, 152, 822, 889, 1123
Bse118I RCCGGY 1 cut(s) 646
Bse1I ACTGG 1 cut(s) 1383
Bse3DI GCAATG 1 cut(s) 205
Bse8I GATNNNNATC 1 cut(s) 426
BseAI TCCGGA 1 cut(s) 640
BseBI CCWGG 1 cut(s) 257
BseDI CCNNGG 5 cut(s) 51, 186, 579, 906, 1095
BseGI GGATG 4 cut(s) 391, 671, 917, 1072
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 6 cut(s) 93, 151, 152, 822, 889, 1123
BseMI GCAATG 1 cut(s) 205
BseMII CTCAG 2 cut(s) 675, 1245
BseNI ACTGG 1 cut(s) 1383
BseRI GAGGAG 3 cut(s) 594, 658, 1510
BseSI GKGCMC 2 cut(s) 546, 1262
BseXI GCAGC 2 cut(s) 562, 739
BsgI GTGCAG 1 cut(s) 793
Bsh1236I CGCG 1 cut(s) 270
BshNI GGYRCC 2 cut(s) 67, 96
BsiHKAI GWGCWC 1 cut(s) 546
BsiSI CCGG 5 cut(s) 362, 641, 647, 884, 900
BslFI GGGAC 3 cut(s) 127, 329, 944
BslI CCNNNNNNNGG 6 cut(s) 93, 151, 152, 822, 889, 1123
BsmAI GTCTC 2 cut(s) 746, 1243
BsmBI CGTCTC 1 cut(s) 1243
BsmFI GGGAC 3 cut(s) 127, 329, 944
Bsp1286I GDGCHC 2 cut(s) 546, 1262
Bsp13I TCCGGA 1 cut(s) 640
Bsp143I GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1357, 1459
Bsp19I CCATGG 2 cut(s) 906, 1095
BspACI CCGC 6 cut(s) 171, 174, 268, 417, 793, 890
BspCNI CTCAG 2 cut(s) 674, 1246
BspEI TCCGGA 1 cut(s) 640
BspFNI CGCG 1 cut(s) 270
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 9 cut(s) 69, 98, 317, 645, 827, 904, 932, 1101, 1232
BspMI ACCTGC 1 cut(s) 1177
BspPI GGATC 3 cut(s) 120, 1134, 1352
BspT107I GGYRCC 2 cut(s) 67, 96
BsrDI GCAATG 1 cut(s) 205
BsrFI RCCGGY 1 cut(s) 646
BsrI ACTGG 1 cut(s) 1383
BssAI RCCGGY 1 cut(s) 646
BssECI CCNNGG 5 cut(s) 51, 186, 579, 906, 1095
BssMI GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1357, 1459
BssT1I CCWWGG 3 cut(s) 579, 906, 1095
Bst2UI CCWGG 1 cut(s) 257
Bst4CI ACNGT 5 cut(s) 187, 443, 809, 853, 1368
Bst6I CTCTTC 1 cut(s) 1487
BstAPI GCANNNNNTGC 1 cut(s) 771
BstBAI YACGTR 1 cut(s) 1190
BstC8I GCNNGC 2 cut(s) 776, 1195
BstDEI CTNAG 3 cut(s) 661, 1198, 1254
BstDSI CCRYGG 3 cut(s) 186, 906, 1095
BstF5I GGATG 4 cut(s) 391, 671, 917, 1072
BstFNI CGCG 1 cut(s) 270
BstHHI GCGC 2 cut(s) 139, 168
BstKTI GATC 8 cut(s) 115, 424, 430, 562, 1072, 1129, 1360, 1462
BstMAI GTCTC 2 cut(s) 746, 1243
BstMBI GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1357, 1459
BstMWI GCNNNNNNNGC 4 cut(s) 581, 656, 771, 1408
BstNI CCWGG 1 cut(s) 257
BstNSI RCATGY 1 cut(s) 1454
BstSCI CCNGG 3 cut(s) 255, 361, 882
BstSFI CTRYAG 1 cut(s) 613
BstSLI GKGCMC 2 cut(s) 546, 1262
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 2 cut(s) 562, 739
BstX2I RGATCY 3 cut(s) 112, 1126, 1357
BstYI RGATCY 3 cut(s) 112, 1126, 1357
BtgI CCRYGG 3 cut(s) 186, 906, 1095
BtrI CACGTC 1 cut(s) 1237
BtsCI GGATG 4 cut(s) 391, 671, 917, 1072
BtsIMutI CAGTG 3 cut(s) 744, 1320, 1390
BveI ACCTGC 1 cut(s) 1177
Cac8I GCNNGC 2 cut(s) 776, 1195
CciI TCATGA 1 cut(s) 238
CfoI GCGC 2 cut(s) 139, 168
Cfr10I RCCGGY 1 cut(s) 646
Cfr13I GGNCC 2 cut(s) 316, 931
Csp6I GTAC 2 cut(s) 433, 1202
CviQI GTAC 2 cut(s) 433, 1202
DdeI CTNAG 3 cut(s) 661, 1198, 1254
DpnI GATC 8 cut(s) 114, 423, 429, 561, 1071, 1128, 1359, 1461
DpnII GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1357, 1459
DriI GACNNNNNGTC 1 cut(s) 518
Eam1104I CTCTTC 1 cut(s) 1487
Eam1105I GACNNNNNGTC 1 cut(s) 518
EarI CTCTTC 1 cut(s) 1487
EciI GGCGGA 1 cut(s) 905
Eco130I CCWWGG 3 cut(s) 579, 906, 1095
Eco47I GGWCC 2 cut(s) 316, 931
EcoO109I RGGNCCY 1 cut(s) 931
EcoRII CCWGG 1 cut(s) 255
EcoT14I CCWWGG 3 cut(s) 579, 906, 1095
EcoT22I ATGCAT 3 cut(s) 77, 839, 1404
ErhI CCWWGG 3 cut(s) 579, 906, 1095
Esp3I CGTCTC 1 cut(s) 1243
FaqI GGGAC 3 cut(s) 127, 329, 944
FauI CCCGC 3 cut(s) 275, 424, 786
FbaI TGATCA 2 cut(s) 427, 559
Fnu4HI GCNGC 3 cut(s) 174, 576, 728
FokI GGATG 4 cut(s) 398, 658, 924, 1079
Fsp4HI GCNGC 3 cut(s) 174, 576, 728
FspBI CTAG 3 cut(s) 1073, 1406, 1437
FspI TGCGCA 1 cut(s) 167
GlaI GCGC 2 cut(s) 138, 167
GluI GCNGC 3 cut(s) 174, 576, 728
HapII CCGG 5 cut(s) 362, 641, 647, 884, 900
HhaI GCGC 2 cut(s) 139, 168
Hin6I GCGC 2 cut(s) 137, 166
HinP1I GCGC 2 cut(s) 137, 166
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HindIII AAGCTT 1 cut(s) 858
HinfI GANTC 7 cut(s) 102, 599, 665, 712, 734, 1018, 1310
HpaI GTTAAC 1 cut(s) 48
HpaII CCGG 5 cut(s) 362, 641, 647, 884, 900
HphI GGTGA 3 cut(s) 133, 1022, 1356
Hpy166II GTNNAC 2 cut(s) 48, 544
Hpy188I TCNGA 8 cut(s) 55, 112, 284, 598, 664, 697, 1126, 1255
Hpy188III TCNNGA 7 cut(s) 239, 398, 425, 641, 847, 1180, 1406
Hpy8I GTNNAC 2 cut(s) 48, 544
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 5 cut(s) 52, 603, 944, 1049, 1463
HpyCH4III ACNGT 5 cut(s) 187, 443, 809, 853, 1368
HpyCH4IV ACGT 2 cut(s) 1189, 1236
HpyF10VI GCNNNNNNNGC 4 cut(s) 581, 656, 771, 1408
HpyF3I CTNAG 3 cut(s) 661, 1198, 1254
HpySE526I ACGT 2 cut(s) 1189, 1236
HspAI GCGC 2 cut(s) 137, 166
Kpn2I TCCGGA 1 cut(s) 640
Ksp22I TGATCA 2 cut(s) 427, 559
KspAI GTTAAC 1 cut(s) 48
Kzo9I GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1357, 1459
LmnI GCTCC 1 cut(s) 902
Lsp1109I GCAGC 2 cut(s) 562, 739
LweI GCATC 2 cut(s) 1294, 1411
MaeI CTAG 3 cut(s) 1073, 1406, 1437
MaeII ACGT 2 cut(s) 1189, 1236
MaeIII GTNAC 1 cut(s) 453
MalI GATC 8 cut(s) 114, 423, 429, 561, 1071, 1128, 1359, 1461
MboI GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1357, 1459
MboII GAAGA 5 cut(s) 260, 317, 1292, 1313, 1504
MfeI CAATTG 1 cut(s) 1217
MflI RGATCY 3 cut(s) 112, 1126, 1357
MhlI GDGCHC 2 cut(s) 546, 1262
MlyI GAGTC 1 cut(s) 659
MmeI TCCRAC 1 cut(s) 1172
Mph1103I ATGCAT 3 cut(s) 77, 839, 1404
MroI TCCGGA 1 cut(s) 640
MseI TTAA 1 cut(s) 47
MslI CAYNNNNRTG 4 cut(s) 80, 185, 570, 1397
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 5 cut(s) 362, 641, 647, 884, 900
MspR9I CCNGG 3 cut(s) 257, 363, 884
MunI CAATTG 1 cut(s) 1217
MvaI CCWGG 1 cut(s) 257
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 4 cut(s) 581, 656, 771, 1408
NciI CCSGG 2 cut(s) 363, 884
NcoI CCATGG 2 cut(s) 906, 1095
NdeII GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1357, 1459
NlaIV GGNNCC 9 cut(s) 69, 98, 317, 645, 827, 904, 932, 1101, 1232
NsbI TGCGCA 1 cut(s) 167
NsiI ATGCAT 3 cut(s) 77, 839, 1404
NspI RCATGY 1 cut(s) 1454
OliI CACNNNNGTG 1 cut(s) 185
PagI TCATGA 1 cut(s) 238
PaqCI CACCTGC 1 cut(s) 1177
PciI ACATGT 1 cut(s) 1450
PfeI GAWTC 6 cut(s) 102, 599, 712, 734, 1018, 1310
PflMI CCANNNNNTGG 2 cut(s) 93, 822
PkrI GCNGC 3 cut(s) 175, 577, 729
PleI GAGTC 1 cut(s) 659
PpsI GAGTC 1 cut(s) 659
Ppu21I YACGTR 1 cut(s) 1190
PpuMI RGGWCCY 1 cut(s) 931
PscI ACATGT 1 cut(s) 1450
PsiI TTATAA 1 cut(s) 411
Psp5II RGGWCCY 1 cut(s) 931
Psp6I CCWGG 1 cut(s) 255
PspGI CCWGG 1 cut(s) 255
PspN4I GGNNCC 9 cut(s) 69, 98, 317, 645, 827, 904, 932, 1101, 1232
PspPI GGNCC 2 cut(s) 316, 931
PspPPI RGGWCCY 1 cut(s) 931
PsuI RGATCY 3 cut(s) 112, 1126, 1357
RsaI GTAC 2 cut(s) 434, 1203
RsaNI GTAC 2 cut(s) 433, 1202
RseI CAYNNNNRTG 4 cut(s) 80, 185, 570, 1397
SaqAI TTAA 1 cut(s) 47
SatI GCNGC 3 cut(s) 174, 576, 728
Sau3AI GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1357, 1459
Sau96I GGNCC 2 cut(s) 316, 931
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 3 cut(s) 257, 363, 884
SduI GDGCHC 2 cut(s) 546, 1262
SfaNI GCATC 2 cut(s) 1294, 1411
SfcI CTRYAG 1 cut(s) 613
SinI GGWCC 2 cut(s) 316, 931
SmiMI CAYNNNNRTG 4 cut(s) 80, 185, 570, 1397
SmlI CTYRAG 2 cut(s) 845, 1412
SmoI CTYRAG 2 cut(s) 845, 1412
SsiI CCGC 6 cut(s) 171, 174, 268, 417, 793, 890
SspI AATATT 1 cut(s) 1062
SspMI CTAG 3 cut(s) 1073, 1406, 1437
StyD4I CCNGG 3 cut(s) 255, 361, 882
StyI CCWWGG 3 cut(s) 579, 906, 1095
TaaI ACNGT 5 cut(s) 187, 443, 809, 853, 1368
TaiI ACGT 2 cut(s) 1192, 1239
TaqI TCGA 2 cut(s) 715, 1506
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 6 cut(s) 102, 599, 712, 734, 1018, 1310
Tru1I TTAA 1 cut(s) 47
Tru9I TTAA 1 cut(s) 47
TscAI CASTG 3 cut(s) 751, 1320, 1390
TseI GCWGC 2 cut(s) 575, 727
TspDTI ATGAA 7 cut(s) 227, 642, 962, 1017, 1236, 1364, 1461
TspRI CASTG 3 cut(s) 751, 1320, 1390
Van91I CCANNNNNTGG 2 cut(s) 93, 822
VneI GTGCAC 1 cut(s) 542
VpaK11BI GGWCC 2 cut(s) 316, 931
XapI RAATTY 3 cut(s) 7, 19, 128
XbaI TCTAGA 1 cut(s) 1405
XceI RCATGY 1 cut(s) 1454
XcmI CCANNNNNNNNNTGG 2 cut(s) 184, 1390
XspI CTAG 3 cut(s) 1073, 1406, 1437
Zsp2I ATGCAT 3 cut(s) 77, 839, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.