Rh2CG161800

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000947
Physical Location & Seq
Reverse (-)
14270373 .. 14271784
1412 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG161800.1

Sequence Viewer

Length: 1377 bp
ATGAGCGTGTCCAAGTTATGGCACAGAATAGCATCAGATCTTTGGGTGAGGAGATTTTGGGCGCAGTCGCCTCCTTTGGGTGTTTACTTTCGCACCATCCAGAATCCTTCGAGTCCTTGCATGCTCACCATGGTGGACAAGGTGCCGTCTTATTTGCCTAGCATTAATTATATCTCACTATATAATTATCATAACATGGATGATAACCTCGAAAGCACCAGTGGTTATCATCTCGGTACGTTATTTAATGGCTTAAGTGACCTGTTTGAGGGCTATAAGCGACGGTGGCAATTTTATCGACATTATAAACATGGTCACGATGATCATGATGATCAGTATCTACACGGCTGCAATGGATTGCTTCTGTTATTGAAATCGGGAACTTATCAGTTTTATGTGTGCAATCCCATTATCACACAACAAGTCCCCGTACCTAAAGCTTGTGTCCACCAAAACCATGAGCAGTTTTGTGCTGCCTTGGCTTTTGATCCTTCTGAATCTCCTCGGCACTACAGAGTTGTGAGGATTGATTACTCTGAATCCGTTTCATCTACCTCAAACTCTGTCATGTTTGACATATTTTCATCTAAATACGGACAGTGGGTAAGACACAGGTTGCAACTCGACCCAGGTTTTACTGAGGGGTTCGAAAAGGTAAAGCTGTGCAGGCAATTTTTTTACTTGCGGGGCAAGTTATATAGCTTAGCCATGTCCTGGAAGATTTTATGCATTGACCTCAAAACTGTTGAAGCTTCAGCCCTTGAACTTCCTGTCCCGGAGGCCAATAAGAACGGAGCGATGGGATGTCTTGGGGTGTCGATGGACCTTCTCTGCTATATAAAGCGTATAAAGCGTATGCCTTCCAGTAGGTTTCACGATCTGGTAGTTTGGTACTATGATGATCGTTGTGAATCTAGTGAATGGACTCGCAGATATAATGTTAGTTGCGGCTTGTTGGGTTATGGAATAAAGTATCAAGGGTATGATTATGATGATACTGTGGAACCCTACGCAATAAGTCCTAGTTCTGATGTCTTGTTCTTTGGCACTCCCAACTTGATTAGTTGCTACCACCTCAAAAGCGAAAAGTACAAGCCCGTGTGTCACCTATCTCCCTCTAAAATTGTACCAGATTACTTCTGGGCATTGCGTGCTTGCTTCGTACCTTTCTACCAGTTGGGAAACAAAGCAGTGGTTCCTGAGGGGAAGGGCATAGAAATCTTGACTGCAGAAGCACAATGTGAAGAAGAGAAAGATGTTGACAAAGTTGAAGTGATAAACGTCAAACTATGTGATCGTCATAAAAAGCCGCTGCCACCGTTGATTTATCCTAGCGAAATTACTGAAATGTATTTGGAATTTGAGTGTCCCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

458

Amino Acids

53.08

Weight (kDa)

6.75

Isoelectric Point (pI)

58.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 104 - 317 2.2e-11 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 112 - 269 1.9e-08 F-box associated beta propeller domain
Beta-prop_AT5G49610-like PF23635 117 - 257 3.1e-06 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 306
AccB1I GGYRCC 1 cut(s) 142
AccB7I CCANNNNNTGG 2 cut(s) 18, 714
AciI CCGC 3 cut(s) 685, 948, 1310
AclWI GGATC 1 cut(s) 482
AcsI RAATTY 1 cut(s) 1358
AcuI CTGAAG 1 cut(s) 738
AdeI CACNNNGTG 1 cut(s) 1241
AfaI GTAC 6 cut(s) 238, 432, 893, 1091, 1128, 1164
AfiI CCNNNNNNNGG 4 cut(s) 18, 77, 268, 714
AflII CTTAAG 1 cut(s) 253
AgsI TTSAA 4 cut(s) 373, 749, 764, 1271
AjnI CCWGG 2 cut(s) 628, 713
AleI CACNNNNGTG 1 cut(s) 131
AloI GAACNNNNNNTCC 2 cut(s) 756, 788
AluBI AGCT 4 cut(s) 440, 661, 702, 752
AluI AGCT 4 cut(s) 440, 661, 702, 752
AlwI GGATC 1 cut(s) 482
AoxI GGCC 1 cut(s) 780
ApeKI GCWGC 3 cut(s) 348, 473, 1312
ApoI RAATTY 1 cut(s) 1358
AseI ATTAAT 1 cut(s) 165
AspLEI GCGC 1 cut(s) 64
AspS9I GGNCC 1 cut(s) 823
AsuC2I CCSGG 1 cut(s) 776
AsuHPI GGTGA 3 cut(s) 58, 118, 1097
AsuII TTCGAA 1 cut(s) 648
AvaII GGWCC 1 cut(s) 823
AxyI CCTNAGG 1 cut(s) 1200
BanI GGYRCC 1 cut(s) 142
BbvI GCAGC 3 cut(s) 335, 460, 1299
BccI CCATC 3 cut(s) 104, 793, 814
BceAI ACGGC 2 cut(s) 130, 361
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 2 cut(s) 630, 715
BclI TGATCA 2 cut(s) 322, 331
BcnI CCSGG 1 cut(s) 776
BfaI CTAG 4 cut(s) 159, 915, 1023, 1332
BfmI CTRYAG 2 cut(s) 511, 1227
BfrI CTTAAG 1 cut(s) 253
BglII AGATCT 1 cut(s) 37
BisI GCNGC 5 cut(s) 349, 474, 949, 1310, 1313
BlpI GCTNAGC 1 cut(s) 703
BlsI GCNGC 5 cut(s) 350, 475, 950, 1311, 1314
Bme1390I CCNGG 3 cut(s) 630, 715, 776
Bme18I GGWCC 1 cut(s) 823
BmgT120I GGNCC 1 cut(s) 823
BmiI GGNNCC 3 cut(s) 144, 1005, 1197
BmrFI CCNGG 3 cut(s) 630, 715, 776
BmsI GCATC 1 cut(s) 41
Bpu1102I GCTNAGC 1 cut(s) 703
Bpu14I TTCGAA 1 cut(s) 648
BpuMI CCSGG 1 cut(s) 776
BsaBI GATNNNNATC 1 cut(s) 336
BsaJI CCNNGG 4 cut(s) 129, 477, 503, 628
Bsc4I CCNNNNNNNGG 4 cut(s) 18, 77, 268, 714
Bse1I ACTGG 3 cut(s) 219, 864, 1174
Bse21I CCTNAGG 1 cut(s) 1200
Bse3DI GCAATG 2 cut(s) 358, 1145
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 2 cut(s) 630, 715
BseDI CCNNGG 4 cut(s) 129, 477, 503, 628
BseGI GGATG 3 cut(s) 96, 205, 809
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 4 cut(s) 18, 77, 268, 714
BseMI GCAATG 2 cut(s) 358, 1145
BseMII CTCAG 2 cut(s) 630, 1191
BseNI ACTGG 3 cut(s) 219, 864, 1174
BseRI GAGGAG 2 cut(s) 64, 492
BseXI GCAGC 3 cut(s) 335, 460, 1299
BsgI GTGCAG 1 cut(s) 685
BshFI GGCC 1 cut(s) 782
BshNI GGYRCC 1 cut(s) 142
BsiSI CCGG 1 cut(s) 776
BslFI GGGAC 3 cut(s) 410, 758, 1353
BslI CCNNNNNNNGG 4 cut(s) 18, 77, 268, 714
BsmFI GGGAC 3 cut(s) 410, 758, 1353
BsnI GGCC 1 cut(s) 782
Bsp119I TTCGAA 1 cut(s) 648
Bsp143I GATC 7 cut(s) 37, 322, 331, 487, 877, 901, 1294
Bsp1720I GCTNAGC 1 cut(s) 703
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 3 cut(s) 685, 948, 1310
BspANI GGCC 1 cut(s) 782
BspCNI CTCAG 2 cut(s) 631, 1192
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 3 cut(s) 144, 1005, 1197
BspMAI CTGCAG 1 cut(s) 1231
BspPI GGATC 1 cut(s) 482
BspT104I TTCGAA 1 cut(s) 648
BspT107I GGYRCC 1 cut(s) 142
BspTI CTTAAG 1 cut(s) 253
BsrDI GCAATG 2 cut(s) 358, 1145
BsrI ACTGG 3 cut(s) 219, 864, 1174
BssECI CCNNGG 4 cut(s) 129, 477, 503, 628
BssMI GATC 7 cut(s) 37, 322, 331, 487, 877, 901, 1294
BssT1I CCWWGG 2 cut(s) 129, 477
Bst2UI CCWGG 2 cut(s) 630, 715
Bst4CI ACNGT 5 cut(s) 285, 600, 745, 1000, 1320
Bst6I CTCTTC 1 cut(s) 1242
BstAFI CTTAAG 1 cut(s) 253
BstAPI GCANNNNNTGC 1 cut(s) 1151
BstBI TTCGAA 1 cut(s) 648
BstC8I GCNNGC 4 cut(s) 122, 668, 1152, 1156
BstDEI CTNAG 3 cut(s) 639, 703, 1200
BstDSI CCRYGG 1 cut(s) 129
BstENI CCTNNNNNAGG 1 cut(s) 266
BstF5I GGATG 3 cut(s) 96, 205, 809
BstHHI GCGC 1 cut(s) 64
BstKTI GATC 7 cut(s) 40, 325, 334, 490, 880, 904, 1297
BstMBI GATC 7 cut(s) 37, 322, 331, 487, 877, 901, 1294
BstMWI GCNNNNNNNGC 5 cut(s) 286, 479, 667, 850, 1151
BstNI CCWGG 2 cut(s) 630, 715
BstNSI RCATGY 1 cut(s) 124
BstSCI CCNGG 3 cut(s) 628, 713, 774
BstSFI CTRYAG 2 cut(s) 511, 1227
BstV1I GCAGC 3 cut(s) 335, 460, 1299
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
Bsu36I CCTNAGG 1 cut(s) 1200
BsuRI GGCC 1 cut(s) 782
BtgI CCRYGG 1 cut(s) 129
BtgZI GCGATG 1 cut(s) 812
BtsCI GGATG 3 cut(s) 96, 205, 809
BtsI GCAGTG 1 cut(s) 1197
BtsIMutI CAGTG 3 cut(s) 226, 605, 1197
Cac8I GCNNGC 4 cut(s) 122, 668, 1152, 1156
CciI TCATGA 1 cut(s) 325
CfoI GCGC 1 cut(s) 64
Cfr13I GGNCC 1 cut(s) 823
Csp6I GTAC 6 cut(s) 237, 431, 892, 1090, 1127, 1163
CviAII CATG 8 cut(s) 121, 130, 196, 311, 326, 458, 568, 709
CviQI GTAC 6 cut(s) 237, 431, 892, 1090, 1127, 1163
DdeI CTNAG 3 cut(s) 639, 703, 1200
DpnI GATC 7 cut(s) 39, 324, 333, 489, 879, 903, 1296
DpnII GATC 7 cut(s) 37, 322, 331, 487, 877, 901, 1294
DraIII CACNNNGTG 1 cut(s) 1241
Eam1104I CTCTTC 1 cut(s) 1242
EarI CTCTTC 1 cut(s) 1242
Eco130I CCWWGG 2 cut(s) 129, 477
Eco47I GGWCC 1 cut(s) 823
Eco57I CTGAAG 1 cut(s) 738
Eco81I CCTNAGG 1 cut(s) 1200
EcoNI CCTNNNNNAGG 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 628, 713
EcoT14I CCWWGG 2 cut(s) 129, 477
EcoT22I ATGCAT 1 cut(s) 731
ErhI CCWWGG 2 cut(s) 129, 477
FaeI CATG 8 cut(s) 124, 133, 199, 314, 329, 461, 571, 712
FaqI GGGAC 3 cut(s) 410, 758, 1353
FatI CATG 8 cut(s) 120, 129, 195, 310, 325, 457, 567, 708
FauI CCCGC 1 cut(s) 678
FbaI TGATCA 2 cut(s) 322, 331
Fnu4HI GCNGC 5 cut(s) 349, 474, 949, 1310, 1313
FokI GGATG 3 cut(s) 83, 212, 816
Fsp4HI GCNGC 5 cut(s) 349, 474, 949, 1310, 1313
FspBI CTAG 4 cut(s) 159, 915, 1023, 1332
GlaI GCGC 1 cut(s) 63
GluI GCNGC 5 cut(s) 349, 474, 949, 1310, 1313
HaeIII GGCC 1 cut(s) 782
HapII CCGG 1 cut(s) 776
HhaI GCGC 1 cut(s) 64
Hin1II CATG 8 cut(s) 124, 133, 199, 314, 329, 461, 571, 712
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HincII GTYRAC 1 cut(s) 1261
HindII GTYRAC 1 cut(s) 1261
HindIII AAGCTT 2 cut(s) 438, 750
HinfI GANTC 6 cut(s) 103, 112, 497, 539, 911, 925
HpaII CCGG 1 cut(s) 776
HphI GGTGA 3 cut(s) 58, 118, 1097
Hpy166II GTNNAC 4 cut(s) 85, 136, 448, 1261
Hpy188I TCNGA 4 cut(s) 37, 496, 538, 1030
Hpy188III TCNNGA 7 cut(s) 100, 317, 326, 378, 875, 1199, 1222
Hpy8I GTNNAC 4 cut(s) 85, 136, 448, 1261
Hpy99I CGWCG 1 cut(s) 285
HpyAV CCTTC 5 cut(s) 117, 501, 836, 870, 1201
HpyCH4III ACNGT 5 cut(s) 285, 600, 745, 1000, 1320
HpyCH4IV ACGT 2 cut(s) 239, 1281
HpyCH4V TGCA 7 cut(s) 120, 351, 402, 619, 666, 729, 1229
HpyF10VI GCNNNNNNNGC 5 cut(s) 286, 479, 667, 850, 1151
HpyF3I CTNAG 3 cut(s) 639, 703, 1200
HpySE526I ACGT 2 cut(s) 239, 1281
Hsp92II CATG 8 cut(s) 124, 133, 199, 314, 329, 461, 571, 712
HspAI GCGC 1 cut(s) 62
Ksp22I TGATCA 2 cut(s) 322, 331
Kzo9I GATC 7 cut(s) 37, 322, 331, 487, 877, 901, 1294
LmnI GCTCC 1 cut(s) 794
Lsp1109I GCAGC 3 cut(s) 335, 460, 1299
LweI GCATC 1 cut(s) 41
MaeI CTAG 4 cut(s) 159, 915, 1023, 1332
MaeII ACGT 2 cut(s) 239, 1281
MaeIII GTNAC 3 cut(s) 257, 314, 1103
MalI GATC 7 cut(s) 39, 324, 333, 489, 879, 903, 1296
MboI GATC 7 cut(s) 37, 322, 331, 487, 877, 901, 1294
MboII GAAGA 3 cut(s) 730, 1256, 1259
MflI RGATCY 1 cut(s) 37
MluCI AATT 8 cut(s) 166, 184, 290, 671, 1122, 1338, 1358, 1372
MlyI GAGTC 2 cut(s) 121, 919
Mph1103I ATGCAT 1 cut(s) 731
MseI TTAA 4 cut(s) 165, 246, 254, 1375
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 1312
MspCI CTTAAG 1 cut(s) 253
MspI CCGG 1 cut(s) 776
MspR9I CCNGG 3 cut(s) 630, 715, 776
MvaI CCWGG 2 cut(s) 630, 715
MwoI GCNNNNNNNGC 5 cut(s) 286, 479, 667, 850, 1151
NciI CCSGG 1 cut(s) 776
NcoI CCATGG 1 cut(s) 129
NdeII GATC 7 cut(s) 37, 322, 331, 487, 877, 901, 1294
NlaIII CATG 8 cut(s) 124, 133, 199, 314, 329, 461, 571, 712
NlaIV GGNNCC 3 cut(s) 144, 1005, 1197
NmeAIII GCCGAG 1 cut(s) 484
NmuCI GTSAC 3 cut(s) 257, 314, 1103
NsiI ATGCAT 1 cut(s) 731
NspI RCATGY 1 cut(s) 124
NspV TTCGAA 1 cut(s) 648
OliI CACNNNNGTG 1 cut(s) 131
PaeI GCATGC 1 cut(s) 124
PagI TCATGA 1 cut(s) 325
PfeI GAWTC 4 cut(s) 103, 497, 539, 911
PflMI CCANNNNNTGG 2 cut(s) 18, 714
PfoI TCCNGGA 2 cut(s) 713, 774
PkrI GCNGC 5 cut(s) 350, 475, 950, 1311, 1314
PleI GAGTC 2 cut(s) 120, 919
PpsI GAGTC 2 cut(s) 120, 919
PshBI ATTAAT 1 cut(s) 165
PsiI TTATAA 1 cut(s) 306
Psp6I CCWGG 2 cut(s) 628, 713
PspGI CCWGG 2 cut(s) 628, 713
PspN4I GGNNCC 3 cut(s) 144, 1005, 1197
PspPI GGNCC 1 cut(s) 823
PstI CTGCAG 1 cut(s) 1231
PsuI RGATCY 1 cut(s) 37
RsaI GTAC 6 cut(s) 238, 432, 893, 1091, 1128, 1164
RsaNI GTAC 6 cut(s) 237, 431, 892, 1090, 1127, 1163
RseI CAYNNNNRTG 1 cut(s) 131
SaqAI TTAA 4 cut(s) 165, 246, 254, 1375
SatI GCNGC 5 cut(s) 349, 474, 949, 1310, 1313
Sau3AI GATC 7 cut(s) 37, 322, 331, 487, 877, 901, 1294
Sau96I GGNCC 1 cut(s) 823
SchI GAGTC 2 cut(s) 121, 919
ScrFI CCNGG 3 cut(s) 630, 715, 776
SfaNI GCATC 1 cut(s) 41
SfcI CTRYAG 2 cut(s) 511, 1227
SfuI TTCGAA 1 cut(s) 648
SinI GGWCC 1 cut(s) 823
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 253
SmoI CTYRAG 1 cut(s) 253
SphI GCATGC 1 cut(s) 124
Sse9I AATT 8 cut(s) 166, 184, 290, 671, 1122, 1338, 1358, 1372
SsiI CCGC 3 cut(s) 685, 948, 1310
SspMI CTAG 4 cut(s) 159, 915, 1023, 1332
StyD4I CCNGG 3 cut(s) 628, 713, 774
StyI CCWWGG 2 cut(s) 129, 477
TaaI ACNGT 5 cut(s) 285, 600, 745, 1000, 1320
TaiI ACGT 2 cut(s) 242, 1284
TaqI TCGA 6 cut(s) 110, 210, 298, 624, 648, 818
TasI AATT 8 cut(s) 166, 184, 290, 671, 1122, 1338, 1358, 1372
TatI WGTACW 1 cut(s) 1089
TauI GCSGC 2 cut(s) 951, 1312
TfiI GAWTC 4 cut(s) 103, 497, 539, 911
Tru1I TTAA 4 cut(s) 165, 246, 254, 1375
Tru9I TTAA 4 cut(s) 165, 246, 254, 1375
TscAI CASTG 3 cut(s) 226, 605, 1197
TseFI GTSAC 3 cut(s) 257, 314, 1103
TseI GCWGC 3 cut(s) 348, 473, 1312
Tsp45I GTSAC 3 cut(s) 257, 314, 1103
TspDTI ATGAA 2 cut(s) 537, 573
TspGWI ACGGA 3 cut(s) 532, 609, 807
TspRI CASTG 3 cut(s) 226, 605, 1197
Van91I CCANNNNNTGG 2 cut(s) 18, 714
Vha464I CTTAAG 1 cut(s) 253
VpaK11BI GGWCC 1 cut(s) 823
VspI ATTAAT 1 cut(s) 165
XagI CCTNNNNNAGG 1 cut(s) 266
XapI RAATTY 1 cut(s) 1358
XceI RCATGY 1 cut(s) 124
XcmI CCANNNNNNNNNTGG 1 cut(s) 1137
XspI CTAG 4 cut(s) 159, 915, 1023, 1332
Zsp2I ATGCAT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.