Rroxscaffold_2G00140500

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000947
Physical Location & Seq
Reverse (-)
78716338 .. 78718357
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00140500.1

Sequence Viewer

Length: 1488 bp
ATGGGGAATTTATATTTGGAAGTGTGGGCGGAGATTTTTAGTAGATTATCAACGGAGAAAGACTTGGTGACATGCATGAAGGTGTCGAAGTCATGGCACCGAATATCATCTGATGTTTGGGCGCGGAGATTTTGGCCGCAATCGGCTCCTTTGGGTCTATACTTCCGCACCATCCAGACTCCGTTGAGTCCTGACATGCGTAGCCCCACGGACAAGGAGCCATCTTATTTGCCTCGCATGAATTATATCTCTCTATATAATTATCATGTCATGGAAGATAACCTTGAAAGCACACGCACTGCTGTCTCTACTTTCTTTAATGATTCAACTGGCGCTTTTAGGAGATCAAAGAGAAAGTGGCAGATATATAGATATTATGAATGGGATGATGATGAAGATGATCTTGATGAGTATCTAGATTGTTGCAATGGATTACTTCTGTTATTGAAGTCGGGAACTTATCAGTTCTATGTGTGCAACCCCATCACCACACAACAAGTCCCCGTACCTAAAGCCTGTGTCCACCAAAACCATGAGCATTTTTGTGCTGCCTTGGCTTTTGACCCTTCGGAATCTCCTCATCACTATAGAGTTGTGAGGATTGATTACTCTGAATCCGTTTCATCAAACTCAAACTCAGCCCTGCTTGATATCTTTTGGTCTGAATACGGACAGTGGGTTAGACATAGGTTGCAACTTGACCCAGGTTTTACTGAGGGGTTTGAGAAGGTGAAGTTGTGCAGGCAATTTGTTTACATGCGGGGCAAGTTATACAGCATAGCCATGTCATGGAAGCTTTTATGCATTGACCTCAAAACTGTTGAAGCTTCGACCCTTGAACTTCCTCTTCATGATGCCGATAAGACCGGAGCAATGGGATGTCTCGGTGTGTCGATGGACCTTCTATGCTATATAAAGCGTATGCATGATACTTCTGATGATCTGCATGATCTGGTAGTTTGGTACTACGATGATCGTTGTGAATCTGGTGAATGGATTCTCAGATATAGTGTTTCTTGCTGCTTGTTGGGATGTGCAATTAAGTATCAAGGGTTTAAGTATGATGATACCGTGGAGCCTTATGCTATAAGTTCAAGTTCTGATGTCTTGTTCTTTGGCACTTCCAATTTGATTTGTTGCTACCATCTCAAAAGCGAAAAGATCAAATTCGTGTGTCATGCAGATTGCTGTACAATAGATACACCTGGAAATTTCTGGATATTGCGTGCTTTCTTCGTACCTTTCTTCCAGTTCCGAAAGAGAAATAGCGGTTCTACTCTCTTACCAATGGTTTCAGATGACATAGAGATTTTGACTGCGGAAACAGTATGTGAAGAAATGAAAGATGATGAATATGTTGAAGTGATAAACGTTAATTTATGTGATCGTCATGAAGAGCCATTGCCGCCGCTGATTTATCCTAGTGAAATAACTGAAATGCATGTGGAGTTTAGTCATCCGGAGACTTCAAGTTCCGATGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

495

Amino Acids

57.56

Weight (kDa)

5.01

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 3 - 39 8.4e-06 F-box domain
F-box-like PF12937 7 - 43 1.2e-07 F-box-like
FBA_1 PF07734 132 - 321 3e-08 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 137 - 294 3.3e-07 KIB1-4 beta-propeller
b-prop_At3g26010-like PF24750 138 - 345 3.5e-11 F-box protein At3g26010-like, beta-propeller
Beta-prop_AT5G49610-like PF23635 139 - 393 2e-09 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 96
AccB7I CCANNNNNTGG 1 cut(s) 789
AccII CGCG 1 cut(s) 124
AccIII TCCGGA 1 cut(s) 1459
AciI CCGC 9 cut(s) 29, 124, 137, 166, 760, 1269, 1319, 1406, 1409
AclI AACGTT 1 cut(s) 1371
AcoI YGGCCR 1 cut(s) 134
AcsI RAATTY 3 cut(s) 7, 1166, 1210
AfaI GTAC 4 cut(s) 507, 965, 1192, 1239
AfiI CCNNNNNNNGG 1 cut(s) 789
AgsI TTSAA 8 cut(s) 287, 327, 448, 824, 839, 1095, 1361, 1470
AjnI CCWGG 2 cut(s) 703, 1204
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AluBI AGCT 2 cut(s) 796, 827
AluI AGCT 2 cut(s) 796, 827
Alw26I GTCTC 3 cut(s) 310, 887, 1457
Aor13HI TCCGGA 1 cut(s) 1459
AoxI GGCC 1 cut(s) 134
ApeKI GCWGC 2 cut(s) 548, 1020
ApoI RAATTY 3 cut(s) 7, 1166, 1210
Asp700I GAANNNNTTC 1 cut(s) 996
AspLEI GCGC 2 cut(s) 124, 335
AspS9I GGNCC 1 cut(s) 898
AsuHPI GGTGA 4 cut(s) 79, 478, 742, 1001
AvaII GGWCC 1 cut(s) 898
BanI GGYRCC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 535, 1007
BccI CCATC 5 cut(s) 179, 229, 491, 889, 1152
BciT130I CCWGG 2 cut(s) 705, 1206
BcoDI GTCTC 3 cut(s) 310, 887, 1457
BfaI CTAG 2 cut(s) 416, 1422
BfmI CTRYAG 1 cut(s) 586
BfoI RGCGCY 1 cut(s) 336
BisI GCNGC 5 cut(s) 137, 549, 1021, 1406, 1409
BlsI GCNGC 5 cut(s) 138, 550, 1022, 1407, 1410
Bme1390I CCNGG 2 cut(s) 705, 1206
Bme18I GGWCC 1 cut(s) 898
BmgT120I GGNCC 1 cut(s) 898
BmiI GGNNCC 4 cut(s) 98, 147, 219, 1077
BmrFI CCNGG 2 cut(s) 705, 1206
BmsI GCATC 1 cut(s) 844
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 4 cut(s) 207, 552, 703, 1071
BsaWI WCCGGW 2 cut(s) 866, 1459
Bsc4I CCNNNNNNNGG 1 cut(s) 789
Bse1I ACTGG 2 cut(s) 334, 1249
Bse3DI GCAATG 3 cut(s) 433, 879, 1400
Bse8I GATNNNNATC 1 cut(s) 411
BseAI TCCGGA 1 cut(s) 1459
BseBI CCWGG 2 cut(s) 705, 1206
BseDI CCNNGG 4 cut(s) 207, 552, 703, 1071
BseGI GGATG 5 cut(s) 171, 391, 884, 1037, 1456
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 1 cut(s) 789
BseMI GCAATG 3 cut(s) 433, 879, 1400
BseMII CTCAG 3 cut(s) 651, 705, 1015
BseNI ACTGG 2 cut(s) 334, 1249
BseRI GAGGAG 1 cut(s) 567
BseXI GCAGC 2 cut(s) 535, 1007
BsgI GTGCAG 1 cut(s) 760
Bsh1236I CGCG 1 cut(s) 124
BshFI GGCC 1 cut(s) 136
BshNI GGYRCC 1 cut(s) 96
BsiSI CCGG 2 cut(s) 867, 1460
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 1 cut(s) 789
BsmAI GTCTC 3 cut(s) 310, 887, 1457
BsmFI GGGAC 1 cut(s) 485
BsnI GGCC 1 cut(s) 136
Bsp13I TCCGGA 1 cut(s) 1459
Bsp1407I TGTACA 1 cut(s) 1190
Bsp143I GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
BspACI CCGC 9 cut(s) 29, 124, 137, 166, 760, 1269, 1319, 1406, 1409
BspANI GGCC 1 cut(s) 136
BspCNI CTCAG 3 cut(s) 650, 706, 1014
BspEI TCCGGA 1 cut(s) 1459
BspFNI CGCG 1 cut(s) 124
BspHI TCATGA 2 cut(s) 850, 1390
BspLI GGNNCC 4 cut(s) 98, 147, 219, 1077
BspQI GCTCTTC 1 cut(s) 1389
BspT107I GGYRCC 1 cut(s) 96
BsrDI GCAATG 3 cut(s) 433, 879, 1400
BsrGI TGTACA 1 cut(s) 1190
BsrI ACTGG 2 cut(s) 334, 1249
BssECI CCNNGG 4 cut(s) 207, 552, 703, 1071
BssMI GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 2 cut(s) 705, 1206
Bst4CI ACNGT 4 cut(s) 675, 820, 1072, 1327
Bst6I CTCTTC 2 cut(s) 852, 1389
BstAUI TGTACA 1 cut(s) 1190
BstC8I GCNNGC 2 cut(s) 743, 1227
BstDEI CTNAG 3 cut(s) 637, 714, 1001
BstDSI CCRYGG 2 cut(s) 207, 1071
BstF5I GGATG 5 cut(s) 171, 391, 884, 1037, 1456
BstFNI CGCG 1 cut(s) 124
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 2 cut(s) 124, 335
BstKTI GATC 7 cut(s) 347, 403, 943, 952, 976, 1164, 1387
BstMAI GTCTC 3 cut(s) 310, 887, 1457
BstMBI GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
BstMWI GCNNNNNNNGC 2 cut(s) 554, 1405
BstNI CCWGG 2 cut(s) 705, 1206
BstNSI RCATGY 4 cut(s) 75, 199, 760, 1445
BstSCI CCNGG 2 cut(s) 703, 1204
BstSFI CTRYAG 1 cut(s) 586
BstUI CGCG 1 cut(s) 124
BstV1I GCAGC 2 cut(s) 535, 1007
BsuRI GGCC 1 cut(s) 136
BtgI CCRYGG 2 cut(s) 207, 1071
BtsCI GGATG 5 cut(s) 171, 391, 884, 1037, 1456
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 2 cut(s) 297, 680
Cac8I GCNNGC 2 cut(s) 743, 1227
CciI TCATGA 2 cut(s) 850, 1390
CfoI GCGC 2 cut(s) 124, 335
Cfr13I GGNCC 1 cut(s) 898
Csp6I GTAC 4 cut(s) 506, 964, 1191, 1238
CviQI GTAC 4 cut(s) 506, 964, 1191, 1238
DdeI CTNAG 3 cut(s) 637, 714, 1001
DpnI GATC 7 cut(s) 346, 402, 942, 951, 975, 1163, 1386
DpnII GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
EaeI YGGCCR 1 cut(s) 134
Eam1104I CTCTTC 2 cut(s) 852, 1389
EarI CTCTTC 2 cut(s) 852, 1389
EciI GGCGGA 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 552
Eco32I GATATC 1 cut(s) 652
Eco47I GGWCC 1 cut(s) 898
EcoRII CCWGG 2 cut(s) 703, 1204
EcoRV GATATC 1 cut(s) 652
EcoT14I CCWWGG 1 cut(s) 552
EcoT22I ATGCAT 4 cut(s) 77, 806, 927, 1443
ErhI CCWWGG 1 cut(s) 552
FalI AAGNNNNNCTT 4 cut(s) 267, 299, 387, 419
FaqI GGGAC 1 cut(s) 485
FauI CCCGC 1 cut(s) 753
Fnu4HI GCNGC 5 cut(s) 137, 549, 1021, 1406, 1409
FokI GGATG 5 cut(s) 158, 398, 891, 1044, 1443
Fsp4HI GCNGC 5 cut(s) 137, 549, 1021, 1406, 1409
FspBI CTAG 2 cut(s) 416, 1422
GlaI GCGC 2 cut(s) 123, 334
GluI GCNGC 5 cut(s) 137, 549, 1021, 1406, 1409
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 1 cut(s) 136
HapII CCGG 2 cut(s) 867, 1460
HhaI GCGC 2 cut(s) 124, 335
Hin6I GCGC 2 cut(s) 122, 333
HinP1I GCGC 2 cut(s) 122, 333
HindIII AAGCTT 2 cut(s) 794, 825
HinfI GANTC 7 cut(s) 178, 187, 323, 572, 614, 983, 997
HpaII CCGG 2 cut(s) 867, 1460
HphI GGTGA 4 cut(s) 79, 478, 742, 1001
Hpy166II GTNNAC 2 cut(s) 523, 754
Hpy188III TCNNGA 9 cut(s) 175, 191, 404, 416, 453, 851, 1216, 1391, 1460
Hpy8I GTNNAC 2 cut(s) 523, 754
HpyAV CCTTC 4 cut(s) 73, 576, 721, 911
HpyCH4III ACNGT 4 cut(s) 675, 820, 1072, 1327
HpyCH4IV ACGT 1 cut(s) 1371
HpyF10VI GCNNNNNNNGC 2 cut(s) 554, 1405
HpyF3I CTNAG 3 cut(s) 637, 714, 1001
HpySE526I ACGT 1 cut(s) 1371
HspAI GCGC 2 cut(s) 122, 333
Kpn2I TCCGGA 1 cut(s) 1459
Kzo9I GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
LguI GCTCTTC 1 cut(s) 1389
LmnI GCTCC 4 cut(s) 151, 217, 869, 1075
Lsp1109I GCAGC 2 cut(s) 535, 1007
LweI GCATC 1 cut(s) 844
MaeI CTAG 2 cut(s) 416, 1422
MaeII ACGT 1 cut(s) 1371
MaeIII GTNAC 1 cut(s) 67
MalI GATC 7 cut(s) 346, 402, 942, 951, 975, 1163, 1386
MboI GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
MboII GAAGA 7 cut(s) 287, 407, 839, 1225, 1237, 1346, 1406
MluCI AATT 9 cut(s) 7, 241, 259, 746, 1038, 1126, 1166, 1210, 1375
MlyI GAGTC 2 cut(s) 172, 196
MnlI CCTC 6 cut(s) 243, 588, 591, 709, 821, 855
Mph1103I ATGCAT 4 cut(s) 77, 806, 927, 1443
MroI TCCGGA 1 cut(s) 1459
MroXI GAANNNNTTC 1 cut(s) 996
MseI TTAA 4 cut(s) 318, 1041, 1056, 1374
MslI CAYNNNNRTG 3 cut(s) 80, 543, 782
MspA1I CMGCKG 1 cut(s) 1411
MspI CCGG 2 cut(s) 867, 1460
MspR9I CCNGG 2 cut(s) 705, 1206
MvaI CCWGG 2 cut(s) 705, 1206
MvnI CGCG 1 cut(s) 124
MwoI GCNNNNNNNGC 2 cut(s) 554, 1405
NdeII GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
NlaIV GGNNCC 4 cut(s) 98, 147, 219, 1077
NmuCI GTSAC 1 cut(s) 67
NsiI ATGCAT 4 cut(s) 77, 806, 927, 1443
NspI RCATGY 4 cut(s) 75, 199, 760, 1445
PagI TCATGA 2 cut(s) 850, 1390
PciSI GCTCTTC 1 cut(s) 1389
PdmI GAANNNNTTC 1 cut(s) 996
PfeI GAWTC 5 cut(s) 323, 572, 614, 983, 997
PflMI CCANNNNNTGG 1 cut(s) 789
PkrI GCNGC 5 cut(s) 138, 550, 1022, 1407, 1410
PleI GAGTC 2 cut(s) 172, 195
PpsI GAGTC 2 cut(s) 172, 195
Psp1406I AACGTT 1 cut(s) 1371
Psp6I CCWGG 2 cut(s) 703, 1204
PspGI CCWGG 2 cut(s) 703, 1204
PspN4I GGNNCC 4 cut(s) 98, 147, 219, 1077
PspPI GGNCC 1 cut(s) 898
RsaI GTAC 4 cut(s) 507, 965, 1192, 1239
RsaNI GTAC 4 cut(s) 506, 964, 1191, 1238
RseI CAYNNNNRTG 3 cut(s) 80, 543, 782
SapI GCTCTTC 1 cut(s) 1389
SaqAI TTAA 4 cut(s) 318, 1041, 1056, 1374
SatI GCNGC 5 cut(s) 137, 549, 1021, 1406, 1409
Sau3AI GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
Sau96I GGNCC 1 cut(s) 898
SchI GAGTC 2 cut(s) 172, 196
ScrFI CCNGG 2 cut(s) 705, 1206
SfaNI GCATC 1 cut(s) 844
SfcI CTRYAG 1 cut(s) 586
SinI GGWCC 1 cut(s) 898
SmiMI CAYNNNNRTG 3 cut(s) 80, 543, 782
Sse9I AATT 9 cut(s) 7, 241, 259, 746, 1038, 1126, 1166, 1210, 1375
SsiI CCGC 9 cut(s) 29, 124, 137, 166, 760, 1269, 1319, 1406, 1409
SspMI CTAG 2 cut(s) 416, 1422
StyD4I CCNGG 2 cut(s) 703, 1204
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 4 cut(s) 675, 820, 1072, 1327
TaiI ACGT 1 cut(s) 1374
TaqI TCGA 3 cut(s) 86, 830, 893
TasI AATT 9 cut(s) 7, 241, 259, 746, 1038, 1126, 1166, 1210, 1375
TatI WGTACW 1 cut(s) 1190
TauI GCSGC 3 cut(s) 139, 1408, 1411
TfiI GAWTC 5 cut(s) 323, 572, 614, 983, 997
Tru1I TTAA 4 cut(s) 318, 1041, 1056, 1374
Tru9I TTAA 4 cut(s) 318, 1041, 1056, 1374
TscAI CASTG 2 cut(s) 304, 680
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 2 cut(s) 548, 1020
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 9 cut(s) 92, 254, 393, 408, 612, 839, 1355, 1365, 1407
TspGWI ACGGA 5 cut(s) 68, 171, 224, 607, 684
TspRI CASTG 2 cut(s) 304, 680
Van91I CCANNNNNTGG 1 cut(s) 789
VpaK11BI GGWCC 1 cut(s) 898
XapI RAATTY 3 cut(s) 7, 1166, 1210
XbaI TCTAGA 1 cut(s) 415
XceI RCATGY 4 cut(s) 75, 199, 760, 1445
XmnI GAANNNNTTC 1 cut(s) 996
XspI CTAG 2 cut(s) 416, 1422
Zsp2I ATGCAT 4 cut(s) 77, 806, 927, 1443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.