Rorug02G0109000

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000947
Physical Location & Seq
Forward (+)
9392418 .. 9395816
3399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0109000.1

Sequence Viewer

Length: 1266 bp
ATGTCGTGCTCCTCTTCATCGGGGTCGGAGGAGGACGATGAGGGTATCGACTCTTACAGAAAAGGAGGGTACCACGCCGTCAGGATCGGCGATCAGTTCGCCGGCGGCCGATATGTTGCTCAGAGAAAACTGGGTTGGGGTCAGTTCTCCACCGTCTGGCTCGCATACGACACCACCAACTCTACGTACGTTGCTCTTAAGATTCAAAAAAGTGCAGCTCAATTTGCTCAAGCTGCTCTCCATGAGATTCAAATTCTTACGGCTATTGCGGAAGGTGACTCCTCGAATTCAAAGTGTGTTGTACGACTGATTGACCATTTTAAGCATGCAGGCCCAAATGGGCAGCATTTATGCATGGTCCTTGAGTTCCTTGGTGATAGCTTACTTCGGCTGATCAAGTACAACCGGTATAAAGGTCTTAAGTTGAATAATGTTAGGGAGATCTGCAAATGTATATTGATGGGTCTGGATTATTTGCATAGAGAACTTGGTATAATCCACACTGACTTAAAACCAGAAAATATTCTTCTCTTTAACACCATTGATCCCACCAAAGATCCCGTCAGGTCTAAATTCACCCCAATTCTTGAAAGGCCTGAAGGAAACCTGAATGGTGGAGTCACCATGAGCCTAATTGAGAAAAAACTCAAAAGGAGGGCAAGGAGAGCAGTTGCTAAGATTTCCTTGAGAAGGGAATCTATGGGAGGAGGTGAAGCTCCCAAGTCTGAAAGAAACATGGATGGGGTTGATGTAAGGTGCAAGGTTGTCGATTTTGGGAATGCATGTTGGGCTGACAAGCAGCTTGCAGAAGAGATACAAACGAGGCAGTATAGAGCTCCTGAGGTTATTCTCCGATCTGGTTATACCTACTCAGTGGATATGTGGTCATTCGCTTGCACAGCCTTTGAGCTTGCGACAGGTGACATGTTGTTTGCTCCGAAGATGGGACAAGGCTTTAGTGAGGATGAGGATCACCTTGCTTTGATGATGGAGGTCCTTGGAAAGATGCCTCGAAAGGTAGCCATTGGAGGAGCTCGTTCCAAGGATTATTTCGACAGACATGGCGATCTAAAGAGAATTCGGAGGCTGAAATTTTGGCCACTTCATAAATTACTGATCGATAGATACAACTTTTCCGAGACTGATGCTCGGGAGTTTGCGGAGTTCCTCTGTCCCATTTTCGACTTTGCACCAGAGAAGCGGCCAACCGCTCAGCAGTGCCTTCAACATCCGTGGCTCAATTGCATTACTACGACTCAGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

421

Amino Acids

47.65

Weight (kDa)

8.74

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 38 - 178 1.4e-22 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 41 - 178 4.4e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 252 - 413 1.3e-20 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 69
AccB1I GGYRCC 1 cut(s) 69
AccB7I CCANNNNNTGG 1 cut(s) 156
AccBSI CCGCTC 1 cut(s) 1211
AciI CCGC 5 cut(s) 105, 269, 1160, 1201, 1209
AclWI GGATC 4 cut(s) 92, 539, 551, 978
AcoI YGGCCR 3 cut(s) 106, 1097, 1202
AcsI RAATTY 5 cut(s) 252, 286, 572, 1077, 1091
AcuI CTGAAG 1 cut(s) 618
AfaI GTAC 4 cut(s) 71, 188, 303, 401
AfiI CCNNNNNNNGG 3 cut(s) 156, 690, 944
AflII CTTAAG 2 cut(s) 197, 419
AflIII ACRYGT 1 cut(s) 924
AgeI ACCGGT 1 cut(s) 405
AgsI TTSAA 6 cut(s) 206, 251, 291, 427, 590, 1226
AjuI GAANNNNNNNTTGG 4 cut(s) 118, 150, 1034, 1066
AluBI AGCT 8 cut(s) 218, 233, 381, 716, 802, 836, 910, 1034
AluI AGCT 8 cut(s) 218, 233, 381, 716, 802, 836, 910, 1034
Alw21I GWGCWC 3 cut(s) 11, 838, 1036
Alw26I GTCTC 1 cut(s) 1133
AlwI GGATC 4 cut(s) 92, 539, 551, 978
Ama87I CYCGRG 1 cut(s) 1149
AoxI GGCC 5 cut(s) 106, 331, 593, 1097, 1202
ApeKI GCWGC 4 cut(s) 215, 233, 343, 799
ApoI RAATTY 5 cut(s) 252, 286, 572, 1077, 1091
ArsI GACNNNNNNTTYG 4 cut(s) 546, 578, 914, 946
AsiGI ACCGGT 1 cut(s) 405
Asp700I GAANNNNTTC 1 cut(s) 522
Asp718I GGTACC 1 cut(s) 69
AspS9I GGNCC 3 cut(s) 332, 358, 994
AsuHPI GGTGA 7 cut(s) 287, 386, 568, 613, 722, 932, 965
AvaI CYCGRG 1 cut(s) 1149
AvaII GGWCC 2 cut(s) 358, 994
AxyI CCTNAGG 1 cut(s) 840
BalI TGGCCA 1 cut(s) 1099
BanI GGYRCC 1 cut(s) 69
BanII GRGCYC 2 cut(s) 838, 1036
Bbv12I GWGCWC 3 cut(s) 11, 838, 1036
BbvI GCAGC 4 cut(s) 220, 227, 355, 811
BccI CCATC 4 cut(s) 454, 734, 937, 982
BceAI ACGGC 2 cut(s) 62, 276
BcgI CGANNNNNNTGC 4 cut(s) 748, 782, 1127, 1161
BclI TGATCA 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 1133
BfaI CTAG 1 cut(s) 1264
BfrI CTTAAG 2 cut(s) 197, 419
BglII AGATCT 1 cut(s) 441
BisI GCNGC 6 cut(s) 106, 216, 234, 344, 800, 1202
BlpI GCTNAGC 1 cut(s) 1212
BlsI GCNGC 6 cut(s) 107, 217, 235, 345, 801, 1203
Bme18I GGWCC 2 cut(s) 358, 994
BmeT110I CYCGRG 1 cut(s) 1149
BmgT120I GGNCC 3 cut(s) 332, 358, 994
BmiI GGNNCC 1 cut(s) 71
BmrI ACTGGG 1 cut(s) 140
BmsI GCATC 2 cut(s) 996, 1135
BmuI ACTGGG 1 cut(s) 140
Bpu1102I GCTNAGC 1 cut(s) 1212
BpuEI CTTGAG 3 cut(s) 213, 383, 706
Bsa29I ATCGAT 1 cut(s) 1119
BsaAI YACGTR 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 969
BsaJI CCNNGG 4 cut(s) 370, 997, 1041, 1232
BsaWI WCCGGW 1 cut(s) 405
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 690, 944
Bse118I RCCGGY 2 cut(s) 101, 405
Bse1I ACTGG 1 cut(s) 135
Bse21I CCTNAGG 1 cut(s) 840
Bse8I GATNNNNATC 1 cut(s) 969
BseCI ATCGAT 1 cut(s) 1119
BseDI CCNNGG 4 cut(s) 370, 997, 1041, 1232
BseGI GGATG 3 cut(s) 745, 970, 1228
BseJI GATNNNNATC 1 cut(s) 969
BseLI CCNNNNNNNGG 3 cut(s) 156, 690, 944
BseMII CTCAG 4 cut(s) 134, 831, 885, 1226
BseNI ACTGG 1 cut(s) 135
BseRI GAGGAG 4 cut(s) 44, 271, 720, 1044
BseX3I CGGCCG 1 cut(s) 106
BseXI GCAGC 4 cut(s) 220, 227, 355, 811
BsgI GTGCAG 1 cut(s) 234
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 5 cut(s) 108, 333, 595, 1099, 1204
BshNI GGYRCC 1 cut(s) 69
BshTI ACCGGT 1 cut(s) 405
BshVI ATCGAT 1 cut(s) 1119
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 3 cut(s) 11, 838, 1036
BsiHKCI CYCGRG 1 cut(s) 1149
BsiSI CCGG 2 cut(s) 102, 406
BsiWI CGTACG 1 cut(s) 186
BslFI GGGAC 2 cut(s) 960, 1158
BslI CCNNNNNNNGG 3 cut(s) 156, 690, 944
BsmAI GTCTC 1 cut(s) 1133
BsmFI GGGAC 2 cut(s) 960, 1158
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 5 cut(s) 108, 333, 595, 1099, 1204
BsoBI CYCGRG 1 cut(s) 1149
Bsp1286I GDGCHC 3 cut(s) 11, 838, 1036
Bsp1720I GCTNAGC 1 cut(s) 1212
BspACI CCGC 5 cut(s) 105, 269, 1160, 1201, 1209
BspANI GGCC 5 cut(s) 108, 333, 595, 1099, 1204
BspCNI CTCAG 4 cut(s) 133, 832, 884, 1225
BspDI ATCGAT 1 cut(s) 1119
BspLI GGNNCC 1 cut(s) 71
BspPI GGATC 4 cut(s) 92, 539, 551, 978
BspT107I GGYRCC 1 cut(s) 69
BspTI CTTAAG 2 cut(s) 197, 419
BsrBI CCGCTC 1 cut(s) 1211
BsrFI RCCGGY 2 cut(s) 101, 405
BsrI ACTGG 1 cut(s) 135
BssAI RCCGGY 2 cut(s) 101, 405
BssECI CCNNGG 4 cut(s) 370, 997, 1041, 1232
BssT1I CCWWGG 3 cut(s) 370, 997, 1041
Bst4CI ACNGT 1 cut(s) 154
Bst6I CTCTTC 2 cut(s) 19, 804
BstAFI CTTAAG 2 cut(s) 197, 419
BstBAI YACGTR 1 cut(s) 186
BstC8I GCNNGC 7 cut(s) 103, 162, 327, 331, 804, 895, 912
BstDEI CTNAG 6 cut(s) 120, 675, 840, 871, 1212, 1257
BstDSI CCRYGG 1 cut(s) 1232
BstENI CCTNNNNNAGG 1 cut(s) 688
BstF5I GGATG 3 cut(s) 745, 970, 1228
BstMAI GTCTC 1 cut(s) 1133
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 5 cut(s) 224, 233, 665, 788, 899
BstNSI RCATGY 3 cut(s) 329, 786, 928
BstSNI TACGTA 1 cut(s) 186
BstV1I GCAGC 4 cut(s) 220, 227, 355, 811
BstX2I RGATCY 2 cut(s) 441, 556
BstYI RGATCY 2 cut(s) 441, 556
BstZI CGGCCG 1 cut(s) 106
Bsu15I ATCGAT 1 cut(s) 1119
Bsu36I CCTNAGG 1 cut(s) 840
BsuRI GGCC 5 cut(s) 108, 333, 595, 1099, 1204
BsuTUI ATCGAT 1 cut(s) 1119
BtgI CCRYGG 1 cut(s) 1232
BtsCI GGATG 3 cut(s) 745, 970, 1228
BtsI GCAGTG 1 cut(s) 1223
BtsIMutI CAGTG 3 cut(s) 501, 879, 1223
Cac8I GCNNGC 7 cut(s) 103, 162, 327, 331, 804, 895, 912
Cfr10I RCCGGY 2 cut(s) 101, 405
Cfr13I GGNCC 3 cut(s) 332, 358, 994
ClaI ATCGAT 1 cut(s) 1119
Csp6I GTAC 4 cut(s) 70, 187, 302, 400
CspAI ACCGGT 1 cut(s) 405
CspCI CAANNNNNGTGG 2 cut(s) 1214, 1249
CviAII CATG 8 cut(s) 242, 326, 355, 625, 736, 783, 925, 1061
CviQI GTAC 4 cut(s) 70, 187, 302, 400
DdeI CTNAG 6 cut(s) 120, 675, 840, 871, 1212, 1257
EaeI YGGCCR 3 cut(s) 106, 1097, 1202
EagI CGGCCG 1 cut(s) 106
Eam1104I CTCTTC 2 cut(s) 19, 804
EarI CTCTTC 2 cut(s) 19, 804
Ecl136II GAGCTC 2 cut(s) 836, 1034
EclXI CGGCCG 1 cut(s) 106
Eco105I TACGTA 1 cut(s) 186
Eco130I CCWWGG 3 cut(s) 370, 997, 1041
Eco147I AGGCCT 1 cut(s) 595
Eco24I GRGCYC 2 cut(s) 838, 1036
Eco47I GGWCC 2 cut(s) 358, 994
Eco52I CGGCCG 1 cut(s) 106
Eco53kI GAGCTC 2 cut(s) 836, 1034
Eco57I CTGAAG 1 cut(s) 618
Eco81I CCTNAGG 1 cut(s) 840
Eco88I CYCGRG 1 cut(s) 1149
EcoICRI GAGCTC 2 cut(s) 836, 1034
EcoNI CCTNNNNNAGG 1 cut(s) 688
EcoO109I RGGNCCY 1 cut(s) 994
EcoRI GAATTC 2 cut(s) 286, 1077
EcoT14I CCWWGG 3 cut(s) 370, 997, 1041
EcoT22I ATGCAT 2 cut(s) 356, 784
EcoT38I GRGCYC 2 cut(s) 838, 1036
ErhI CCWWGG 3 cut(s) 370, 997, 1041
FaeI CATG 8 cut(s) 245, 329, 358, 628, 739, 786, 928, 1064
FalI AAGNNNNNCTT 2 cut(s) 668, 700
FaqI GGGAC 2 cut(s) 960, 1158
FatI CATG 8 cut(s) 241, 325, 354, 624, 735, 782, 924, 1060
FbaI TGATCA 1 cut(s) 393
Fnu4HI GCNGC 6 cut(s) 106, 216, 234, 344, 800, 1202
FokI GGATG 3 cut(s) 752, 977, 1215
FriOI GRGCYC 2 cut(s) 838, 1036
Fsp4HI GCNGC 6 cut(s) 106, 216, 234, 344, 800, 1202
FspBI CTAG 1 cut(s) 1264
GluI GCNGC 6 cut(s) 106, 216, 234, 344, 800, 1202
HaeIII GGCC 5 cut(s) 108, 333, 595, 1099, 1204
HapII CCGG 2 cut(s) 102, 406
Hin1II CATG 8 cut(s) 245, 329, 358, 628, 739, 786, 928, 1064
HinfI GANTC 7 cut(s) 50, 202, 247, 278, 618, 695, 1255
HpaII CCGG 2 cut(s) 102, 406
HphI GGTGA 7 cut(s) 287, 386, 568, 613, 722, 932, 965
Hpy188I TCNGA 7 cut(s) 28, 123, 727, 854, 939, 1083, 1138
Hpy188III TCNNGA 5 cut(s) 82, 467, 587, 839, 1151
HpyAV CCTTC 4 cut(s) 266, 593, 684, 1232
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 2 cut(s) 185, 189
HpyF10VI GCNNNNNNNGC 5 cut(s) 224, 233, 665, 788, 899
HpyF3I CTNAG 6 cut(s) 120, 675, 840, 871, 1212, 1257
HpySE526I ACGT 2 cut(s) 185, 189
Hsp92II CATG 8 cut(s) 245, 329, 358, 628, 739, 786, 928, 1064
KpnI GGTACC 1 cut(s) 73
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
Ksp22I TGATCA 1 cut(s) 393
LmnI GCTCC 5 cut(s) 14, 721, 841, 940, 1031
Lsp1109I GCAGC 4 cut(s) 220, 227, 355, 811
LweI GCATC 2 cut(s) 996, 1135
MaeI CTAG 1 cut(s) 1264
MaeII ACGT 2 cut(s) 185, 189
MaeIII GTNAC 3 cut(s) 275, 619, 920
MbiI CCGCTC 1 cut(s) 1211
MboII GAAGA 4 cut(s) 6, 518, 821, 952
MfeI CAATTG 1 cut(s) 1240
MflI RGATCY 2 cut(s) 441, 556
MhlI GDGCHC 3 cut(s) 11, 838, 1036
MlsI TGGCCA 1 cut(s) 1099
MluNI TGGCCA 1 cut(s) 1099
MlyI GAGTC 4 cut(s) 44, 272, 627, 1249
MmeI TCCRAC 1 cut(s) 6
Mox20I TGGCCA 1 cut(s) 1099
Mph1103I ATGCAT 2 cut(s) 356, 784
MreI CGCCGGCG 1 cut(s) 101
MroNI GCCGGC 1 cut(s) 101
MroXI GAANNNNTTC 1 cut(s) 522
MscI TGGCCA 1 cut(s) 1099
MseI TTAA 5 cut(s) 198, 321, 420, 509, 534
Msp20I TGGCCA 1 cut(s) 1099
MspCI CTTAAG 2 cut(s) 197, 419
MspI CCGG 2 cut(s) 102, 406
MunI CAATTG 1 cut(s) 1240
Mva1269I GAATGC 1 cut(s) 784
MwoI GCNNNNNNNGC 5 cut(s) 224, 233, 665, 788, 899
NaeI GCCGGC 1 cut(s) 103
NgoMIV GCCGGC 1 cut(s) 101
NlaIII CATG 8 cut(s) 245, 329, 358, 628, 739, 786, 928, 1064
NlaIV GGNNCC 1 cut(s) 71
NmuCI GTSAC 3 cut(s) 275, 619, 920
NsiI ATGCAT 2 cut(s) 356, 784
NspI RCATGY 3 cut(s) 329, 786, 928
PaeI GCATGC 1 cut(s) 329
PceI AGGCCT 1 cut(s) 595
PciI ACATGT 1 cut(s) 924
PctI GAATGC 1 cut(s) 784
PdiI GCCGGC 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 3 cut(s) 202, 247, 695
Pfl23II CGTACG 1 cut(s) 186
PflMI CCANNNNNTGG 1 cut(s) 156
PinAI ACCGGT 1 cut(s) 405
PkrI GCNGC 6 cut(s) 107, 217, 235, 345, 801, 1203
PleI GAGTC 4 cut(s) 44, 272, 626, 1249
PpsI GAGTC 4 cut(s) 44, 272, 626, 1249
Ppu21I YACGTR 1 cut(s) 186
PpuMI RGGWCCY 1 cut(s) 994
PscI ACATGT 1 cut(s) 924
Psp124BI GAGCTC 2 cut(s) 838, 1036
Psp5II RGGWCCY 1 cut(s) 994
PspLI CGTACG 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 71
PspPI GGNCC 3 cut(s) 332, 358, 994
PspPPI RGGWCCY 1 cut(s) 994
PsuI RGATCY 2 cut(s) 441, 556
RsaI GTAC 4 cut(s) 71, 188, 303, 401
RsaNI GTAC 4 cut(s) 70, 187, 302, 400
SacI GAGCTC 2 cut(s) 838, 1036
SaqAI TTAA 5 cut(s) 198, 321, 420, 509, 534
SatI GCNGC 6 cut(s) 106, 216, 234, 344, 800, 1202
Sau96I GGNCC 3 cut(s) 332, 358, 994
SchI GAGTC 4 cut(s) 44, 272, 627, 1249
SduI GDGCHC 3 cut(s) 11, 838, 1036
SfaNI GCATC 2 cut(s) 996, 1135
SgrAI CRCCGGYG 1 cut(s) 101
SinI GGWCC 2 cut(s) 358, 994
SmlI CTYRAG 5 cut(s) 197, 228, 362, 419, 685
SmoI CTYRAG 5 cut(s) 197, 228, 362, 419, 685
SnaBI TACGTA 1 cut(s) 186
SphI GCATGC 1 cut(s) 329
SseBI AGGCCT 1 cut(s) 595
SsiI CCGC 5 cut(s) 105, 269, 1160, 1201, 1209
SspI AATATT 1 cut(s) 523
SspMI CTAG 1 cut(s) 1264
SstI GAGCTC 2 cut(s) 838, 1036
StuI AGGCCT 1 cut(s) 595
StyI CCWWGG 3 cut(s) 370, 997, 1041
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 2 cut(s) 188, 192
TaqI TCGA 7 cut(s) 48, 284, 768, 1012, 1053, 1119, 1182
TatI WGTACW 1 cut(s) 399
TauI GCSGC 2 cut(s) 108, 1204
TfiI GAWTC 3 cut(s) 202, 247, 695
Tru1I TTAA 5 cut(s) 198, 321, 420, 509, 534
Tru9I TTAA 5 cut(s) 198, 321, 420, 509, 534
TscAI CASTG 3 cut(s) 508, 879, 1223
TseFI GTSAC 3 cut(s) 275, 619, 920
TseI GCWGC 4 cut(s) 215, 233, 343, 799
Tsp45I GTSAC 3 cut(s) 275, 619, 920
TspDTI ATGAA 2 cut(s) 6, 1094
TspGWI ACGGA 1 cut(s) 1221
TspRI CASTG 3 cut(s) 508, 879, 1223
Van91I CCANNNNNTGG 1 cut(s) 156
Vha464I CTTAAG 2 cut(s) 197, 419
VpaK11BI GGWCC 2 cut(s) 358, 994
XagI CCTNNNNNAGG 1 cut(s) 688
XapI RAATTY 5 cut(s) 252, 286, 572, 1077, 1091
XceI RCATGY 3 cut(s) 329, 786, 928
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 1 cut(s) 1264
Zsp2I ATGCAT 2 cut(s) 356, 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.