Rorug02G0108900

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000947
Physical Location & Seq
Forward (+)
9380557 .. 9382424
1868 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0108900.1

Sequence Viewer

Length: 1272 bp
ATGGTGGTGTTCGACAAGGACGCAACCTCATCCTCCCAAACCCTCCCTCCTTTCCCACGCGAGGACACGCCTCTCCTCGGAAAACGCTCCCATCTCTCCTCACAGTCCAAGACATTCGCCAACGTCTTCATCGCCATCGTCGGCTCCGGCGTGCTGGGCCTCCCTTACACCTTCAAGAAAACCGGATGGGTTTTCGGCTCTGTTATGCTCTTCTCTGTAGCTTTTCTTACCTACCACTGTATGATGCTTCTGGTCAAAACCCGCCGGAGGCTCGAGTCCGATATGGCTCTCTCCAAGGTCAACTCTTTCGGCGATCTGGCCTTTGCCGTTTGCGGCCCAGTTGGGCGATTTGCGGTTGATGTCAGCATCGTGCTGGCTCAGGCTGGGTTCTGTGGTAGTTACATGATTTTCATATCGAATAGTTTGGCATATGTGTTCAACTATTCTGGCCCGGATCGAGTTCTGGGTATGAGTCCGAAAAGCTTGTATCTTTGGGGGTGTTTTCCATTTCAATTGGGGTTGAATTCGATTCCCACACTGACCCATTTGGCTCCTTTGAGTATTTTTGCTGATGTGGTTGATCTTGGAGCTATGGGAGTTGTTATGGTTGAGGACGTAATGATTTTTCTGCAGAATCGGCCTGCTTTGCAAGCATTTGGGGGCTTTTCGGTTTTCTTCTATGGTCTTGGTGTGGCTGTTTATGCATTTGAAGGGATTGGGATGATATTGCCATTGGAATCTGAGACCAGAGACAAGGACAAGTTTGGCAAAGTGTTGGCTTTGGTTATGGCCTTCATTGCTTTGTTGTATGGAAGCTTTGGGGTCCTGGGTTACTTTGCTTTTGGGGAAGAGACTAAAGACATAATCACTACCAACTTCGGGCAAGGCTTGGTGAGCACTCTAGTCCAGTTGGGTCTCTGCATAAACTTGTTCTTTACTCTACCATTGATGATGCACCCGGTTTTCGAGGTTGTGGAGAGGAGGTTTTGGGATGGGGGGTACTGCTTGTGGCTGAGGTGGGTTATGGTGTTTGGGGTCTGCTTGGTGGCTCTTACAGTGCCTAATTTTGCAGACTTTTTGTCACTGGTGGGGAGCAGTGTTTGTGTGGCTTTAGGGTTTGTGTTTCCTGCTTTGTTCCATTTGATGGTGTTCAAGGAAGAGTTGGGGTGTCATGGGATGGTTTTGGATGGGACTATTGTGGTTATTGGGGTGATTATTGGAGTCTCTGGAACTTGGTCTTCAATAGCAGAGATTGTTGCACCCAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

423

Amino Acids

46.06

Weight (kDa)

6.08

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 34 - 415 1.5e-74 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1144
AccII CGCG 1 cut(s) 60
AciI CCGC 3 cut(s) 262, 333, 353
AclWI GGATC 1 cut(s) 462
AcsI RAATTY 1 cut(s) 523
AfaI GTAC 1 cut(s) 1001
AfiI CCNNNNNNNGG 5 cut(s) 61, 77, 267, 879, 1144
AgsI TTSAA 7 cut(s) 175, 439, 512, 523, 710, 1153, 1242
AhdI GACNNNNNGTC 1 cut(s) 1078
AjnI CCWGG 1 cut(s) 825
AluBI AGCT 5 cut(s) 221, 483, 590, 816, 1268
AluI AGCT 5 cut(s) 221, 483, 590, 816, 1268
Alw21I GWGCWC 1 cut(s) 899
Alw26I GTCTC 5 cut(s) 737, 744, 845, 920, 1228
AlwI GGATC 1 cut(s) 462
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 6 cut(s) 157, 318, 334, 448, 638, 789
ApoI RAATTY 1 cut(s) 523
AspS9I GGNCC 4 cut(s) 157, 335, 449, 823
AsuC2I CCSGG 2 cut(s) 452, 959
AsuHPI GGTGA 2 cut(s) 904, 1222
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 1 cut(s) 823
BbsI GAAGAC 2 cut(s) 118, 1230
Bbv12I GWGCWC 1 cut(s) 899
BbvCI CCTCAGC 1 cut(s) 1013
BccI CCATC 7 cut(s) 99, 143, 180, 986, 1138, 1171, 1181
BceAI ACGGC 1 cut(s) 311
BciT130I CCWGG 1 cut(s) 827
BcnI CCSGG 2 cut(s) 452, 959
BcoDI GTCTC 5 cut(s) 737, 744, 845, 920, 1228
BfaI CTAG 1 cut(s) 902
BfmI CTRYAG 2 cut(s) 216, 629
BisI GCNGC 1 cut(s) 334
BlsI GCNGC 1 cut(s) 335
Bme1390I CCNGG 3 cut(s) 452, 827, 959
Bme18I GGWCC 1 cut(s) 823
BmeRI GACNNNNNGTC 1 cut(s) 1078
BmeT110I CYCGRG 1 cut(s) 272
BmgT120I GGNCC 4 cut(s) 157, 335, 449, 823
BmiI GGNNCC 3 cut(s) 145, 552, 824
BmrFI CCNGG 3 cut(s) 452, 827, 959
BmrI ACTGGG 1 cut(s) 332
BmsI GCATC 3 cut(s) 234, 375, 942
BmuI ACTGGG 1 cut(s) 332
BpiI GAAGAC 2 cut(s) 118, 1230
Bpu10I CCTNAGC 2 cut(s) 378, 1013
BpuMI CCSGG 2 cut(s) 452, 959
BsaI GGTCTC 2 cut(s) 737, 920
BsaJI CCNNGG 3 cut(s) 76, 294, 826
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 4 cut(s) 31, 61, 968, 998
Bsc4I CCNNNNNNNGG 5 cut(s) 61, 77, 267, 879, 1144
Bse1I ACTGG 3 cut(s) 338, 907, 1089
Bse3DI GCAATG 1 cut(s) 795
BseBI CCWGG 1 cut(s) 827
BseDI CCNNGG 3 cut(s) 76, 294, 826
BseGI GGATG 6 cut(s) 29, 191, 726, 997, 1182, 1192
BseLI CCNNNNNNNGG 5 cut(s) 61, 77, 267, 879, 1144
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 3 cut(s) 392, 732, 1004
BseNI ACTGG 3 cut(s) 338, 907, 1089
BseRI GAGGAG 3 cut(s) 65, 88, 994
BseYI CCCAGC 2 cut(s) 154, 383
Bsh1236I CGCG 1 cut(s) 60
BshFI GGCC 6 cut(s) 159, 320, 336, 450, 640, 791
BsiHKAI GWGCWC 1 cut(s) 899
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 5 cut(s) 147, 183, 265, 452, 959
BslFI GGGAC 1 cut(s) 1204
BslI CCNNNNNNNGG 5 cut(s) 61, 77, 267, 879, 1144
BsmAI GTCTC 5 cut(s) 737, 744, 845, 920, 1228
BsmFI GGGAC 1 cut(s) 1204
BsnI GGCC 6 cut(s) 159, 320, 336, 450, 640, 791
Bso31I GGTCTC 2 cut(s) 737, 920
BsoBI CYCGRG 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 899
Bsp143I GATC 3 cut(s) 313, 454, 580
BspACI CCGC 3 cut(s) 262, 333, 353
BspANI GGCC 6 cut(s) 159, 320, 336, 450, 640, 791
BspCNI CTCAG 3 cut(s) 391, 733, 1005
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 3 cut(s) 145, 552, 824
BspMAI CTGCAG 1 cut(s) 633
BspPI GGATC 1 cut(s) 462
BspQI GCTCTTC 1 cut(s) 215
BspTNI GGTCTC 2 cut(s) 737, 920
BsrDI GCAATG 1 cut(s) 795
BsrI ACTGG 3 cut(s) 338, 907, 1089
BssECI CCNNGG 3 cut(s) 76, 294, 826
BssMI GATC 3 cut(s) 313, 454, 580
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 827
Bst4CI ACNGT 3 cut(s) 105, 239, 1057
Bst6I CTCTTC 3 cut(s) 215, 843, 1152
BstC8I GCNNGC 4 cut(s) 152, 375, 642, 651
BstDEI CTNAG 3 cut(s) 378, 741, 1013
BstF5I GGATG 6 cut(s) 29, 191, 726, 997, 1182, 1192
BstFNI CGCG 1 cut(s) 60
BstKTI GATC 3 cut(s) 316, 457, 583
BstMAI GTCTC 5 cut(s) 737, 744, 845, 920, 1228
BstMBI GATC 3 cut(s) 313, 454, 580
BstMWI GCNNNNNNNGC 8 cut(s) 156, 637, 646, 650, 701, 797, 894, 1265
BstNI CCWGG 1 cut(s) 827
BstSCI CCNGG 3 cut(s) 450, 825, 957
BstSFI CTRYAG 2 cut(s) 216, 629
BstUI CGCG 1 cut(s) 60
BstV2I GAAGAC 2 cut(s) 118, 1230
BsuRI GGCC 6 cut(s) 159, 320, 336, 450, 640, 791
BtgZI GCGATG 1 cut(s) 115
BtsCI GGATG 6 cut(s) 29, 191, 726, 997, 1182, 1192
BtsI GCAGTG 1 cut(s) 1102
BtsIMutI CAGTG 5 cut(s) 235, 536, 1062, 1082, 1102
Cac8I GCNNGC 4 cut(s) 152, 375, 642, 651
Cfr13I GGNCC 4 cut(s) 157, 335, 449, 823
CseI GACGC 1 cut(s) 29
Csp6I GTAC 1 cut(s) 1000
CviAII CATG 2 cut(s) 403, 1172
CviQI GTAC 1 cut(s) 1000
DdeI CTNAG 3 cut(s) 378, 741, 1013
DpnI GATC 3 cut(s) 315, 456, 582
DpnII GATC 3 cut(s) 313, 454, 580
DriI GACNNNNNGTC 1 cut(s) 1078
Eam1104I CTCTTC 3 cut(s) 215, 843, 1152
Eam1105I GACNNNNNGTC 1 cut(s) 1078
EarI CTCTTC 3 cut(s) 215, 843, 1152
Eco130I CCWWGG 1 cut(s) 294
Eco31I GGTCTC 2 cut(s) 737, 920
Eco47I GGWCC 1 cut(s) 823
Eco88I CYCGRG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 823
EcoRI GAATTC 1 cut(s) 523
EcoRII CCWGG 1 cut(s) 825
EcoT14I CCWWGG 1 cut(s) 294
EcoT22I ATGCAT 1 cut(s) 706
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 2 cut(s) 406, 1175
FaqI GGGAC 1 cut(s) 1204
FatI CATG 2 cut(s) 402, 1171
FauI CCCGC 1 cut(s) 269
FauNDI CATATG 1 cut(s) 430
Fnu4HI GCNGC 1 cut(s) 334
FokI GGATG 6 cut(s) 16, 198, 733, 1004, 1189, 1199
Fsp4HI GCNGC 1 cut(s) 334
FspBI CTAG 1 cut(s) 902
GluI GCNGC 1 cut(s) 334
GsaI CCCAGC 2 cut(s) 158, 387
HaeIII GGCC 6 cut(s) 159, 320, 336, 450, 640, 791
HapII CCGG 5 cut(s) 147, 183, 265, 452, 959
HgaI GACGC 1 cut(s) 29
Hin1II CATG 2 cut(s) 406, 1175
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HindIII AAGCTT 3 cut(s) 481, 814, 1266
HinfI GANTC 6 cut(s) 275, 472, 529, 634, 737, 1221
HpaII CCGG 5 cut(s) 147, 183, 265, 452, 959
HphI GGTGA 2 cut(s) 904, 1222
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 4 cut(s) 80, 280, 477, 742
Hpy188III TCNNGA 2 cut(s) 175, 1227
Hpy8I GTNNAC 1 cut(s) 301
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 3 cut(s) 181, 704, 802
HpyCH4III ACNGT 3 cut(s) 105, 239, 1057
HpyCH4IV ACGT 2 cut(s) 123, 615
HpyCH4V TGCA 7 cut(s) 631, 649, 704, 921, 955, 1070, 1259
HpyF10VI GCNNNNNNNGC 8 cut(s) 156, 637, 646, 650, 701, 797, 894, 1265
HpyF3I CTNAG 3 cut(s) 378, 741, 1013
HpySE526I ACGT 2 cut(s) 123, 615
Hsp92II CATG 2 cut(s) 406, 1175
Kzo9I GATC 3 cut(s) 313, 454, 580
LguI GCTCTTC 1 cut(s) 215
LmnI GCTCC 5 cut(s) 92, 149, 556, 587, 1092
LweI GCATC 3 cut(s) 234, 375, 942
MaeI CTAG 1 cut(s) 902
MaeII ACGT 2 cut(s) 123, 615
MaeIII GTNAC 3 cut(s) 398, 830, 1080
MalI GATC 3 cut(s) 315, 456, 582
MboI GATC 3 cut(s) 313, 454, 580
MboII GAAGA 6 cut(s) 118, 202, 667, 860, 1169, 1230
MfeI CAATTG 1 cut(s) 512
MhlI GDGCHC 1 cut(s) 899
MluCI AATT 3 cut(s) 512, 523, 1063
MlyI GAGTC 3 cut(s) 284, 481, 1230
Mph1103I ATGCAT 1 cut(s) 706
MspI CCGG 5 cut(s) 147, 183, 265, 452, 959
MspR9I CCNGG 3 cut(s) 452, 827, 959
MunI CAATTG 1 cut(s) 512
MvaI CCWGG 1 cut(s) 827
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 8 cut(s) 156, 637, 646, 650, 701, 797, 894, 1265
NciI CCSGG 2 cut(s) 452, 959
NdeI CATATG 1 cut(s) 430
NdeII GATC 3 cut(s) 313, 454, 580
NlaIII CATG 2 cut(s) 406, 1175
NlaIV GGNNCC 3 cut(s) 145, 552, 824
NmuCI GTSAC 1 cut(s) 1080
NsiI ATGCAT 1 cut(s) 706
PaeR7I CTCGAG 1 cut(s) 272
PciSI GCTCTTC 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PfeI GAWTC 3 cut(s) 529, 634, 737
PflMI CCANNNNNTGG 1 cut(s) 1144
PkrI GCNGC 1 cut(s) 335
PleI GAGTC 3 cut(s) 283, 480, 1229
PpsI GAGTC 3 cut(s) 283, 480, 1229
PpuMI RGGWCCY 1 cut(s) 823
Psp5II RGGWCCY 1 cut(s) 823
Psp6I CCWGG 1 cut(s) 825
PspFI CCCAGC 2 cut(s) 154, 383
PspGI CCWGG 1 cut(s) 825
PspN4I GGNNCC 3 cut(s) 145, 552, 824
PspPI GGNCC 4 cut(s) 157, 335, 449, 823
PspPPI RGGWCCY 1 cut(s) 823
PspXI VCTCGAGB 1 cut(s) 272
PstI CTGCAG 1 cut(s) 633
RsaI GTAC 1 cut(s) 1001
RsaNI GTAC 1 cut(s) 1000
SapI GCTCTTC 1 cut(s) 215
SatI GCNGC 1 cut(s) 334
Sau3AI GATC 3 cut(s) 313, 454, 580
Sau96I GGNCC 4 cut(s) 157, 335, 449, 823
SchI GAGTC 3 cut(s) 284, 481, 1230
ScrFI CCNGG 3 cut(s) 452, 827, 959
SduI GDGCHC 1 cut(s) 899
SfaNI GCATC 3 cut(s) 234, 375, 942
SfcI CTRYAG 2 cut(s) 216, 629
Sfr274I CTCGAG 1 cut(s) 272
SinI GGWCC 1 cut(s) 823
SlaI CTCGAG 1 cut(s) 272
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 3 cut(s) 512, 523, 1063
SsiI CCGC 3 cut(s) 262, 333, 353
SspMI CTAG 1 cut(s) 902
StyD4I CCNGG 3 cut(s) 450, 825, 957
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 3 cut(s) 105, 239, 1057
TaiI ACGT 2 cut(s) 126, 618
TaqI TCGA 6 cut(s) 12, 273, 416, 457, 527, 966
TasI AATT 3 cut(s) 512, 523, 1063
TauI GCSGC 1 cut(s) 336
TfiI GAWTC 3 cut(s) 529, 634, 737
TscAI CASTG 5 cut(s) 242, 543, 1062, 1089, 1102
TseFI GTSAC 1 cut(s) 1080
Tsp45I GTSAC 1 cut(s) 1080
TspDTI ATGAA 3 cut(s) 118, 400, 784
TspRI CASTG 5 cut(s) 242, 543, 1062, 1089, 1102
Van91I CCANNNNNTGG 1 cut(s) 1144
VpaK11BI GGWCC 1 cut(s) 823
XapI RAATTY 1 cut(s) 523
XhoI CTCGAG 1 cut(s) 272
XspI CTAG 1 cut(s) 902
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.