Rh2CG161400

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000947
Physical Location & Seq
Forward (+)
14238032 .. 14240552
2521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG161400.1

Sequence Viewer

Length: 1488 bp
ATGGGGAATTTATATGAGGAATTGTGGGCGGAGATTTTTAGTAGATTATCAACGGAGAAAGACTTGGTGACATGCATGAAGGTGTCGAAGTCATGGCACCGAATATCATCTGATGTTTGGGCGCGGAGATTTTGGGCGCAATCGGCTCCTTTGGGTCTATACTTCCGCACCATCCAGACTCCGTTGAGTCCTGACATGCGTAGCCCCACGGACAAGGAGCCATCTTATTTGCCTCGCATGAATTATATCTCTCTATATAATTATCATGTCATGGAAGATAACCTTGAAAGCACACGCACTTCTGTCTCTAGTTTCTTTAATGATTCAACTGGCGCTTTTAGGAGATCAAAGAGAAAGTGGCAGATATATAGATATTATGAATGGGATGATGGTGAAGATGATCTTGATGAGTATTTAGATTGTTGCAATGGATTACTTCTGTTATTGAAGTCGGGAACTTATCAGTTCTATGTGTGCAACCCCATCACCACACAACAAGTCCCCGTACCTAAAGCCTGTGTCCACCAAAACCATGAGCATTTTTGTGCTGCCTTGGCTTTTGACCCTTCGGAATCTCCTCATCACTATAGAGTTGTGAGGATTGATTACTCTGAATCCGTTTCATCAAACTCAAACTCTGCCCTGCTTGATATCTTTTGGTCTGAATACGGACAGTGGGTTAGACATAGGTTGCAACTTGACCCAGGTTTTACTGAGGGGTTTGAGAAGGTGAAGTTGTGCAGGCAATTTGTTTACATGCGGGGCAAGTTATACAGCATAGCCATGTCATGGAAGCTTTTATGCATTGACCTCAAAACTGTTGAAGCTTCGACCCTTGAACTTCCTCTTCATGATGCCGATAAGACCGGAGCAATGGGATGTCTCGGTGTGTCGATGGACCTTCTATGCTATATAAAGCGTATGCATGATACTTCTGATGATCTGCACGATCTGGTAGTTTGGTACTACGATGATCGTTGTGAATCTGGTGAATGGATTCTTAGATATAGTGTTTCTTGCTGCTTGTTGGGATGTGCAATTAAGTATCAAGGGTTTAAGTATGATGATACCGTGGAGCCTTATGCTATAAGTTCAAGTTCTGATGTTTTGTTCTTTGGCACTTCCAATTTGATTTGTTGCTACCATCTCAAAAGCGAAAAGATCAAATTCGTGTGTCATGCCGATTGCTGTACAATAGATACACCTGGTAATTTCTGGATATTGCGTGCTTTCTTCGTACCTTTCTTCCAGTTCCGAAAGAGAAATAGCGGTTCTACTCTCTTACCAATGGTTTCAGAGGACACAGAGATTTTGACTGCAGAAACAGTATGTGAAGAAATGAAAGATGATGAATATGTTGAAGTGATAAACGTTAATTTATGTGATCGTCATCAAGAGCCATTGCCGCCGTTGATTTATCCTAGTGAAATTACTGAAATGCATGTGGAGTTTAGTCATCCGGAGACTTCAAGTTCCGATGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

495

Amino Acids

57.51

Weight (kDa)

5.06

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 3 - 39 5.4e-06 F-box domain
F-box-like PF12937 6 - 44 1.4e-07 F-box-like
FBA_1 PF07734 134 - 321 3.3e-08 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 137 - 294 3.5e-07 KIB1-4 beta-propeller
b-prop_At3g26010-like PF24750 138 - 345 4.3e-11 F-box protein At3g26010-like, beta-propeller
Beta-prop_AT5G49610-like PF23635 139 - 393 2e-09 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 96
AccB7I CCANNNNNTGG 1 cut(s) 789
AccII CGCG 1 cut(s) 124
AccIII TCCGGA 1 cut(s) 1459
AciI CCGC 6 cut(s) 29, 124, 166, 760, 1269, 1406
AclI AACGTT 1 cut(s) 1371
AcsI RAATTY 2 cut(s) 7, 1166
AfaI GTAC 4 cut(s) 507, 965, 1192, 1239
AfiI CCNNNNNNNGG 1 cut(s) 789
AgsI TTSAA 8 cut(s) 287, 327, 448, 824, 839, 1095, 1361, 1470
AjnI CCWGG 2 cut(s) 703, 1204
AluBI AGCT 2 cut(s) 796, 827
AluI AGCT 2 cut(s) 796, 827
Alw26I GTCTC 3 cut(s) 310, 887, 1457
Aor13HI TCCGGA 1 cut(s) 1459
ApeKI GCWGC 2 cut(s) 548, 1020
ApoI RAATTY 2 cut(s) 7, 1166
Asp700I GAANNNNTTC 1 cut(s) 996
AspLEI GCGC 3 cut(s) 124, 139, 335
AspS9I GGNCC 1 cut(s) 898
AsuHPI GGTGA 5 cut(s) 79, 404, 478, 742, 1001
AvaII GGWCC 1 cut(s) 898
BanI GGYRCC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 535, 1007
BccI CCATC 6 cut(s) 179, 229, 383, 491, 889, 1152
BceAI ACGGC 1 cut(s) 1393
BciT130I CCWGG 2 cut(s) 705, 1206
BcoDI GTCTC 3 cut(s) 310, 887, 1457
BfaI CTAG 2 cut(s) 309, 1422
BfmI CTRYAG 2 cut(s) 586, 1317
BfoI RGCGCY 1 cut(s) 336
BisI GCNGC 3 cut(s) 549, 1021, 1406
BlsI GCNGC 3 cut(s) 550, 1022, 1407
Bme1390I CCNGG 2 cut(s) 705, 1206
Bme18I GGWCC 1 cut(s) 898
BmgT120I GGNCC 1 cut(s) 898
BmiI GGNNCC 4 cut(s) 98, 147, 219, 1077
BmrFI CCNGG 2 cut(s) 705, 1206
BmsI GCATC 1 cut(s) 844
BsaBI GATNNNNATC 1 cut(s) 1389
BsaJI CCNNGG 4 cut(s) 207, 552, 703, 1071
BsaWI WCCGGW 2 cut(s) 866, 1459
Bsc4I CCNNNNNNNGG 1 cut(s) 789
Bse1I ACTGG 2 cut(s) 334, 1249
Bse3DI GCAATG 3 cut(s) 433, 879, 1400
Bse8I GATNNNNATC 1 cut(s) 1389
BseAI TCCGGA 1 cut(s) 1459
BseBI CCWGG 2 cut(s) 705, 1206
BseDI CCNNGG 4 cut(s) 207, 552, 703, 1071
BseGI GGATG 5 cut(s) 171, 391, 884, 1037, 1456
BseJI GATNNNNATC 1 cut(s) 1389
BseLI CCNNNNNNNGG 1 cut(s) 789
BseMI GCAATG 3 cut(s) 433, 879, 1400
BseMII CTCAG 1 cut(s) 705
BseNI ACTGG 2 cut(s) 334, 1249
BseRI GAGGAG 1 cut(s) 567
BseXI GCAGC 2 cut(s) 535, 1007
BsgI GTGCAG 2 cut(s) 760, 929
Bsh1236I CGCG 1 cut(s) 124
BshNI GGYRCC 1 cut(s) 96
BsiSI CCGG 2 cut(s) 867, 1460
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 1 cut(s) 789
BsmAI GTCTC 3 cut(s) 310, 887, 1457
BsmFI GGGAC 1 cut(s) 485
Bsp13I TCCGGA 1 cut(s) 1459
Bsp1407I TGTACA 1 cut(s) 1190
Bsp143I GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
BspACI CCGC 6 cut(s) 29, 124, 166, 760, 1269, 1406
BspCNI CTCAG 1 cut(s) 706
BspEI TCCGGA 1 cut(s) 1459
BspFNI CGCG 1 cut(s) 124
BspHI TCATGA 1 cut(s) 850
BspLI GGNNCC 4 cut(s) 98, 147, 219, 1077
BspMAI CTGCAG 1 cut(s) 1321
BspT107I GGYRCC 1 cut(s) 96
BsrDI GCAATG 3 cut(s) 433, 879, 1400
BsrGI TGTACA 1 cut(s) 1190
BsrI ACTGG 2 cut(s) 334, 1249
BssECI CCNNGG 4 cut(s) 207, 552, 703, 1071
BssMI GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 2 cut(s) 705, 1206
Bst4CI ACNGT 4 cut(s) 675, 820, 1072, 1327
Bst6I CTCTTC 1 cut(s) 852
BstAUI TGTACA 1 cut(s) 1190
BstC8I GCNNGC 2 cut(s) 743, 1227
BstDEI CTNAG 2 cut(s) 714, 1001
BstDSI CCRYGG 2 cut(s) 207, 1071
BstF5I GGATG 5 cut(s) 171, 391, 884, 1037, 1456
BstFNI CGCG 1 cut(s) 124
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 3 cut(s) 124, 139, 335
BstKTI GATC 7 cut(s) 347, 403, 943, 952, 976, 1164, 1387
BstMAI GTCTC 3 cut(s) 310, 887, 1457
BstMBI GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
BstMWI GCNNNNNNNGC 3 cut(s) 143, 554, 1405
BstNI CCWGG 2 cut(s) 705, 1206
BstNSI RCATGY 4 cut(s) 75, 199, 760, 1445
BstSCI CCNGG 2 cut(s) 703, 1204
BstSFI CTRYAG 2 cut(s) 586, 1317
BstUI CGCG 1 cut(s) 124
BstV1I GCAGC 2 cut(s) 535, 1007
BtgI CCRYGG 2 cut(s) 207, 1071
BtsCI GGATG 5 cut(s) 171, 391, 884, 1037, 1456
BtsIMutI CAGTG 1 cut(s) 680
Cac8I GCNNGC 2 cut(s) 743, 1227
CciI TCATGA 1 cut(s) 850
CfoI GCGC 3 cut(s) 124, 139, 335
Cfr13I GGNCC 1 cut(s) 898
CsiI ACCWGGT 1 cut(s) 1204
Csp6I GTAC 4 cut(s) 506, 964, 1191, 1238
CviQI GTAC 4 cut(s) 506, 964, 1191, 1238
DdeI CTNAG 2 cut(s) 714, 1001
DpnI GATC 7 cut(s) 346, 402, 942, 951, 975, 1163, 1386
DpnII GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
Eam1104I CTCTTC 1 cut(s) 852
EarI CTCTTC 1 cut(s) 852
EciI GGCGGA 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 552
Eco32I GATATC 1 cut(s) 652
Eco47I GGWCC 1 cut(s) 898
EcoRII CCWGG 2 cut(s) 703, 1204
EcoRV GATATC 1 cut(s) 652
EcoT14I CCWWGG 1 cut(s) 552
EcoT22I ATGCAT 4 cut(s) 77, 806, 927, 1443
ErhI CCWWGG 1 cut(s) 552
FalI AAGNNNNNCTT 4 cut(s) 267, 299, 387, 419
FaqI GGGAC 1 cut(s) 485
FauI CCCGC 1 cut(s) 753
Fnu4HI GCNGC 3 cut(s) 549, 1021, 1406
FokI GGATG 5 cut(s) 158, 398, 891, 1044, 1443
Fsp4HI GCNGC 3 cut(s) 549, 1021, 1406
FspBI CTAG 2 cut(s) 309, 1422
GlaI GCGC 3 cut(s) 123, 138, 334
GluI GCNGC 3 cut(s) 549, 1021, 1406
HaeII RGCGCY 1 cut(s) 336
HapII CCGG 2 cut(s) 867, 1460
HhaI GCGC 3 cut(s) 124, 139, 335
Hin6I GCGC 3 cut(s) 122, 137, 333
HinP1I GCGC 3 cut(s) 122, 137, 333
HindIII AAGCTT 2 cut(s) 794, 825
HinfI GANTC 7 cut(s) 178, 187, 323, 572, 614, 983, 997
HpaII CCGG 2 cut(s) 867, 1460
HphI GGTGA 5 cut(s) 79, 404, 478, 742, 1001
Hpy166II GTNNAC 2 cut(s) 523, 754
Hpy188I TCNGA 9 cut(s) 112, 571, 613, 664, 937, 1102, 1256, 1297, 1477
Hpy188III TCNNGA 8 cut(s) 175, 191, 404, 453, 851, 1216, 1394, 1460
Hpy8I GTNNAC 2 cut(s) 523, 754
HpyAV CCTTC 4 cut(s) 73, 576, 721, 911
HpyCH4III ACNGT 4 cut(s) 675, 820, 1072, 1327
HpyCH4IV ACGT 1 cut(s) 1371
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 554, 1405
HpyF3I CTNAG 2 cut(s) 714, 1001
HpySE526I ACGT 1 cut(s) 1371
HspAI GCGC 3 cut(s) 122, 137, 333
Kpn2I TCCGGA 1 cut(s) 1459
Kzo9I GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
LmnI GCTCC 4 cut(s) 151, 217, 869, 1075
Lsp1109I GCAGC 2 cut(s) 535, 1007
LweI GCATC 1 cut(s) 844
MabI ACCWGGT 1 cut(s) 1204
MaeI CTAG 2 cut(s) 309, 1422
MaeII ACGT 1 cut(s) 1371
MaeIII GTNAC 1 cut(s) 67
MalI GATC 7 cut(s) 346, 402, 942, 951, 975, 1163, 1386
MboI GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
MboII GAAGA 6 cut(s) 287, 407, 839, 1225, 1237, 1346
MlyI GAGTC 2 cut(s) 172, 196
MnlI CCTC 8 cut(s) 10, 243, 588, 591, 709, 821, 855, 1291
Mph1103I ATGCAT 4 cut(s) 77, 806, 927, 1443
MroI TCCGGA 1 cut(s) 1459
MroXI GAANNNNTTC 1 cut(s) 996
MseI TTAA 4 cut(s) 318, 1041, 1056, 1374
MslI CAYNNNNRTG 3 cut(s) 80, 543, 782
MspI CCGG 2 cut(s) 867, 1460
MspR9I CCNGG 2 cut(s) 705, 1206
MvaI CCWGG 2 cut(s) 705, 1206
MvnI CGCG 1 cut(s) 124
MwoI GCNNNNNNNGC 3 cut(s) 143, 554, 1405
NdeII GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
NlaIV GGNNCC 4 cut(s) 98, 147, 219, 1077
NmuCI GTSAC 1 cut(s) 67
NsiI ATGCAT 4 cut(s) 77, 806, 927, 1443
NspI RCATGY 4 cut(s) 75, 199, 760, 1445
PagI TCATGA 1 cut(s) 850
PdmI GAANNNNTTC 1 cut(s) 996
PfeI GAWTC 5 cut(s) 323, 572, 614, 983, 997
PflMI CCANNNNNTGG 1 cut(s) 789
PkrI GCNGC 3 cut(s) 550, 1022, 1407
PleI GAGTC 2 cut(s) 172, 195
PpsI GAGTC 2 cut(s) 172, 195
Psp1406I AACGTT 1 cut(s) 1371
Psp6I CCWGG 2 cut(s) 703, 1204
PspGI CCWGG 2 cut(s) 703, 1204
PspN4I GGNNCC 4 cut(s) 98, 147, 219, 1077
PspPI GGNCC 1 cut(s) 898
PstI CTGCAG 1 cut(s) 1321
RsaI GTAC 4 cut(s) 507, 965, 1192, 1239
RsaNI GTAC 4 cut(s) 506, 964, 1191, 1238
RseI CAYNNNNRTG 3 cut(s) 80, 543, 782
SaqAI TTAA 4 cut(s) 318, 1041, 1056, 1374
SatI GCNGC 3 cut(s) 549, 1021, 1406
Sau3AI GATC 7 cut(s) 344, 400, 940, 949, 973, 1161, 1384
Sau96I GGNCC 1 cut(s) 898
SchI GAGTC 2 cut(s) 172, 196
ScrFI CCNGG 2 cut(s) 705, 1206
SexAI ACCWGGT 1 cut(s) 1204
SfaNI GCATC 1 cut(s) 844
SfcI CTRYAG 2 cut(s) 586, 1317
SinI GGWCC 1 cut(s) 898
SmiMI CAYNNNNRTG 3 cut(s) 80, 543, 782
SsiI CCGC 6 cut(s) 29, 124, 166, 760, 1269, 1406
SspMI CTAG 2 cut(s) 309, 1422
StyD4I CCNGG 2 cut(s) 703, 1204
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 4 cut(s) 675, 820, 1072, 1327
TaiI ACGT 1 cut(s) 1374
TaqI TCGA 3 cut(s) 86, 830, 893
TatI WGTACW 1 cut(s) 1190
TauI GCSGC 1 cut(s) 1408
TfiI GAWTC 5 cut(s) 323, 572, 614, 983, 997
Tru1I TTAA 4 cut(s) 318, 1041, 1056, 1374
Tru9I TTAA 4 cut(s) 318, 1041, 1056, 1374
TscAI CASTG 1 cut(s) 680
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 2 cut(s) 548, 1020
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 7 cut(s) 92, 254, 393, 612, 839, 1355, 1365
TspGWI ACGGA 5 cut(s) 68, 171, 224, 607, 684
TspRI CASTG 1 cut(s) 680
Van91I CCANNNNNTGG 1 cut(s) 789
VpaK11BI GGWCC 1 cut(s) 898
XapI RAATTY 2 cut(s) 7, 1166
XceI RCATGY 4 cut(s) 75, 199, 760, 1445
XmnI GAANNNNTTC 1 cut(s) 996
XspI CTAG 2 cut(s) 309, 1422
Zsp2I ATGCAT 4 cut(s) 77, 806, 927, 1443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.