Rw2G012160

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000947
Physical Location & Seq
Forward (+)
12522424 .. 12524370
1947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G012160.1

Sequence Viewer

Length: 1494 bp
ATGTGGAATCGGATCCACACACCCAACACATCACGGGCACTTGAATTGCGAATGGGGAATTTATGCGAGGATTTGTGGGTTGAGATCTTGGGTAGACTGTCATCTGAGAAAGACTTGGTGCCATGCATGAGTGTGTCCAAGTTATGGCACAGAATAGCATCAGATCTTTGGGTGAGGAGATTTTGGGCGCAGTCGCCTCCTTTGGGTGTTTACTTCCGCACCATCCGGAATCCTTCGAGTCCTTTCATGCTTTCCATGGTGGACAAGGTGCCGTCTTATTTGCCTAGCATTAATTATATCTCTCTATATAATTATCATAACATGGATGATAACCTCAAAAGCACCAGCGGTTATCATCTCTGTACGTTATTTAATGGCCTAATTGACCTGTTTGAGTGCTATAAGCGAGGTTGGCAATTTTATCGACATTATAAACACGGTCACGATGATCATGATGATCAGTATCTACACGGCTGCAATGGATTACTTCTGTTATTGAAATCGGGAACTTATCAGTTTTATGTGTGCAATCCCATTACCACACAACAAGTCCCCGTACCTAAAGCTTGTGTCCACCAAAACCATGAGCATTTTTGTGCTGCCTTGGCTTTTGACCCTTCTGAATCTCCTTATCACTACAGAGTTGTGAGGATTGATTACTCTGAATCCGTTTCATCTACCTCAAACTCTGTCATGTTTGACATATTTTCATCTATATACGGACAGTGGGTTCGACATAGGTTGCAACTTGACCCAGGTTTTACTGAGGGGTTTGAAAAGGTAAAGCTGTGCAGGCAATTTTTTTACTTGCGGGGCAAGTTATATAGCTTAGCCATGTCATGGAAGATTTTATGCATTGACCTCAAAACTGTTGAAGCTTCAGCCCTTGAACTTCCTGTCCCGGAGGCCAATAAGAACGGAGCGATGGGATGTCTTGGGGTGTCGATGGACGTTCTCTGCTATATAAAGCGTATGCCTTCCAGAAAGTTTCACGATCTGGTAGTTTGGTACTATGATGATCGTTGTGAATCTAGTGAATGGACTCGCAGATATAATGTTAGTTGCGGCTTGTTGGGTTATGGAATAAAGTATCAAGGGTATGATTATGATGATACTGTGGAACCCTATGCAATAAGTCCTAGTTCTGATGTCTTGTTCTTTGGCACTCCCGACTTGATTAGTTGCTACCACCTCAAAAGCGAGAAGTACAAGCCCGTGTGTCATCTATCTCCCTCTAAAATTGTACCTGATTACTTCTGGGTATTGCGTGCTTGCTTTGTACCTTTCTACCAGTTGGGAAACAAAGCAGTGGTTCCTGAGGGGAAGGGCATAGAAATCTTGACTGCAGAAGCACAATGTGAAGAAGAGAAAGATGTTGACAAAGTTGAAGTGATAAACGTCAAACTATGTGATCGTCATAAAAAGCCGTTGCCACCGTTGATTTATCCTAGCGAAATTACTGAAATGTATTTGGAATTTGAGTGTCCCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

497

Amino Acids

57.68

Weight (kDa)

6.41

Isoelectric Point (pI)

56.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 23 - 61 1.5e-06 F-box-like
b-prop_At3g26010-like PF24750 148 - 357 3.8e-12 F-box protein At3g26010-like, beta-propeller
Beta-prop_KIB1-4 PF03478 153 - 312 2.1e-06 KIB1-4 beta-propeller
FBA_1 PF07734 154 - 321 1.5e-07 F-box associated beta propeller domain
Beta-prop_AT5G49610-like PF23635 159 - 301 3.8e-06 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 432
AccB1I GGYRCC 2 cut(s) 118, 268
AccB7I CCANNNNNTGG 2 cut(s) 144, 840
AccI GTMKAC 1 cut(s) 94
AccIII TCCGGA 1 cut(s) 225
AciI CCGC 4 cut(s) 217, 348, 811, 1065
AclWI GGATC 2 cut(s) 7, 20
AcsI RAATTY 2 cut(s) 58, 1475
AcuI CTGAAG 1 cut(s) 864
AdeI CACNNNGTG 1 cut(s) 1358
AfaI GTAC 6 cut(s) 364, 558, 1010, 1208, 1245, 1281
AfiI CCNNNNNNNGG 3 cut(s) 144, 203, 840
AgsI TTSAA 6 cut(s) 44, 499, 776, 875, 890, 1388
AjnI CCWGG 1 cut(s) 754
AloI GAACNNNNNNTCC 4 cut(s) 714, 746, 882, 914
AluBI AGCT 4 cut(s) 566, 787, 828, 878
AluI AGCT 4 cut(s) 566, 787, 828, 878
AlwI GGATC 2 cut(s) 7, 20
Aor13HI TCCGGA 1 cut(s) 225
AoxI GGCC 2 cut(s) 376, 906
ApeKI GCWGC 2 cut(s) 474, 599
ApoI RAATTY 2 cut(s) 58, 1475
ArsI GACNNNNNNTTYG 2 cut(s) 714, 746
AseI ATTAAT 1 cut(s) 291
AspLEI GCGC 1 cut(s) 190
AsuC2I CCSGG 1 cut(s) 902
AsuHPI GGTGA 1 cut(s) 184
AxyI CCTNAGG 1 cut(s) 1317
BaeGI GKGCMC 1 cut(s) 40
BamHI GGATCC 1 cut(s) 12
BanI GGYRCC 2 cut(s) 118, 268
BbvI GCAGC 2 cut(s) 461, 586
BccI CCATC 3 cut(s) 230, 919, 940
BceAI ACGGC 3 cut(s) 256, 487, 1411
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BciT130I CCWGG 1 cut(s) 756
BclI TGATCA 2 cut(s) 448, 457
BcnI CCSGG 1 cut(s) 902
BfaI CTAG 4 cut(s) 285, 1032, 1140, 1449
BfmI CTRYAG 2 cut(s) 637, 1344
BglII AGATCT 2 cut(s) 84, 163
BisI GCNGC 3 cut(s) 475, 600, 1066
BlpI GCTNAGC 1 cut(s) 829
BlsI GCNGC 3 cut(s) 476, 601, 1067
Bme1390I CCNGG 2 cut(s) 756, 902
BmiI GGNNCC 5 cut(s) 14, 120, 270, 1122, 1314
BmrFI CCNGG 2 cut(s) 756, 902
BmsI GCATC 1 cut(s) 167
Bpu1102I GCTNAGC 1 cut(s) 829
BpuMI CCSGG 1 cut(s) 902
BsaBI GATNNNNATC 1 cut(s) 462
BsaJI CCNNGG 3 cut(s) 255, 603, 754
BsaWI WCCGGW 1 cut(s) 225
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 203, 840
Bse1I ACTGG 1 cut(s) 1291
Bse21I CCTNAGG 1 cut(s) 1317
Bse3DI GCAATG 1 cut(s) 484
Bse8I GATNNNNATC 1 cut(s) 462
BseAI TCCGGA 1 cut(s) 225
BseBI CCWGG 1 cut(s) 756
BseDI CCNNGG 3 cut(s) 255, 603, 754
BseGI GGATG 3 cut(s) 222, 331, 935
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 3 cut(s) 144, 203, 840
BseMI GCAATG 1 cut(s) 484
BseMII CTCAG 3 cut(s) 96, 756, 1308
BseNI ACTGG 1 cut(s) 1291
BseRI GAGGAG 1 cut(s) 190
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 2 cut(s) 461, 586
BsgI GTGCAG 1 cut(s) 811
BshFI GGCC 2 cut(s) 378, 908
BshNI GGYRCC 2 cut(s) 118, 268
BsiSI CCGG 2 cut(s) 226, 902
BslFI GGGAC 3 cut(s) 536, 884, 1470
BslI CCNNNNNNNGG 3 cut(s) 144, 203, 840
BsmFI GGGAC 3 cut(s) 536, 884, 1470
BsnI GGCC 2 cut(s) 378, 908
Bsp1286I GDGCHC 1 cut(s) 40
Bsp13I TCCGGA 1 cut(s) 225
Bsp143I GATC 8 cut(s) 12, 84, 163, 448, 457, 994, 1018, 1411
Bsp1720I GCTNAGC 1 cut(s) 829
Bsp19I CCATGG 1 cut(s) 255
BspACI CCGC 4 cut(s) 217, 348, 811, 1065
BspANI GGCC 2 cut(s) 378, 908
BspCNI CTCAG 3 cut(s) 97, 757, 1309
BspEI TCCGGA 1 cut(s) 225
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 5 cut(s) 14, 120, 270, 1122, 1314
BspMAI CTGCAG 1 cut(s) 1348
BspPI GGATC 2 cut(s) 7, 20
BspT107I GGYRCC 2 cut(s) 118, 268
BsrDI GCAATG 1 cut(s) 484
BsrI ACTGG 1 cut(s) 1291
BssECI CCNNGG 3 cut(s) 255, 603, 754
BssMI GATC 8 cut(s) 12, 84, 163, 448, 457, 994, 1018, 1411
BssT1I CCWWGG 2 cut(s) 255, 603
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 6 cut(s) 99, 440, 726, 871, 1117, 1437
Bst6I CTCTTC 1 cut(s) 1359
BstC8I GCNNGC 3 cut(s) 794, 1269, 1273
BstDEI CTNAG 4 cut(s) 105, 765, 829, 1317
BstDSI CCRYGG 1 cut(s) 255
BstF5I GGATG 3 cut(s) 222, 331, 935
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 8 cut(s) 15, 87, 166, 451, 460, 997, 1021, 1414
BstMBI GATC 8 cut(s) 12, 84, 163, 448, 457, 994, 1018, 1411
BstMWI GCNNNNNNNGC 3 cut(s) 412, 605, 793
BstNI CCWGG 1 cut(s) 756
BstSCI CCNGG 2 cut(s) 754, 900
BstSFI CTRYAG 2 cut(s) 637, 1344
BstSLI GKGCMC 1 cut(s) 40
BstV1I GCAGC 2 cut(s) 461, 586
BstX2I RGATCY 3 cut(s) 12, 84, 163
BstYI RGATCY 3 cut(s) 12, 84, 163
Bsu36I CCTNAGG 1 cut(s) 1317
BsuRI GGCC 2 cut(s) 378, 908
BtgI CCRYGG 1 cut(s) 255
BtgZI GCGATG 1 cut(s) 938
BtsCI GGATG 3 cut(s) 222, 331, 935
BtsI GCAGTG 1 cut(s) 1314
BtsIMutI CAGTG 2 cut(s) 731, 1314
Cac8I GCNNGC 3 cut(s) 794, 1269, 1273
CciI TCATGA 1 cut(s) 451
CfoI GCGC 1 cut(s) 190
Csp6I GTAC 6 cut(s) 363, 557, 1009, 1207, 1244, 1280
CviQI GTAC 6 cut(s) 363, 557, 1009, 1207, 1244, 1280
DdeI CTNAG 4 cut(s) 105, 765, 829, 1317
DpnI GATC 8 cut(s) 14, 86, 165, 450, 459, 996, 1020, 1413
DpnII GATC 8 cut(s) 12, 84, 163, 448, 457, 994, 1018, 1411
DraIII CACNNNGTG 1 cut(s) 1358
Eam1104I CTCTTC 1 cut(s) 1359
EarI CTCTTC 1 cut(s) 1359
Eco130I CCWWGG 2 cut(s) 255, 603
Eco57I CTGAAG 1 cut(s) 864
Eco81I CCTNAGG 1 cut(s) 1317
EcoRII CCWGG 1 cut(s) 754
EcoT14I CCWWGG 2 cut(s) 255, 603
EcoT22I ATGCAT 2 cut(s) 128, 857
ErhI CCWWGG 2 cut(s) 255, 603
FaqI GGGAC 3 cut(s) 536, 884, 1470
FauI CCCGC 1 cut(s) 804
FbaI TGATCA 2 cut(s) 448, 457
FblI GTMKAC 1 cut(s) 94
Fnu4HI GCNGC 3 cut(s) 475, 600, 1066
FokI GGATG 3 cut(s) 209, 338, 942
Fsp4HI GCNGC 3 cut(s) 475, 600, 1066
FspBI CTAG 4 cut(s) 285, 1032, 1140, 1449
GlaI GCGC 1 cut(s) 189
GluI GCNGC 3 cut(s) 475, 600, 1066
HaeIII GGCC 2 cut(s) 378, 908
HapII CCGG 2 cut(s) 226, 902
HhaI GCGC 1 cut(s) 190
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HincII GTYRAC 1 cut(s) 1378
HindII GTYRAC 1 cut(s) 1378
HindIII AAGCTT 2 cut(s) 564, 876
HinfI GANTC 7 cut(s) 7, 229, 238, 623, 665, 1028, 1042
HpaII CCGG 2 cut(s) 226, 902
HphI GGTGA 1 cut(s) 184
Hpy166II GTNNAC 5 cut(s) 95, 211, 262, 574, 1378
Hpy188I TCNGA 6 cut(s) 12, 106, 163, 622, 664, 1147
Hpy188III TCNNGA 9 cut(s) 226, 443, 452, 504, 981, 992, 1169, 1316, 1339
Hpy8I GTNNAC 5 cut(s) 95, 211, 262, 574, 1378
HpyAV CCTTC 4 cut(s) 243, 627, 987, 1318
HpyCH4III ACNGT 6 cut(s) 99, 440, 726, 871, 1117, 1437
HpyCH4IV ACGT 3 cut(s) 365, 951, 1398
HpyCH4V TGCA 8 cut(s) 126, 477, 528, 745, 792, 855, 1130, 1346
HpyF10VI GCNNNNNNNGC 3 cut(s) 412, 605, 793
HpyF3I CTNAG 4 cut(s) 105, 765, 829, 1317
HpySE526I ACGT 3 cut(s) 365, 951, 1398
HspAI GCGC 1 cut(s) 188
Kpn2I TCCGGA 1 cut(s) 225
Ksp22I TGATCA 2 cut(s) 448, 457
Kzo9I GATC 8 cut(s) 12, 84, 163, 448, 457, 994, 1018, 1411
LmnI GCTCC 1 cut(s) 920
Lsp1109I GCAGC 2 cut(s) 461, 586
LweI GCATC 1 cut(s) 167
MaeI CTAG 4 cut(s) 285, 1032, 1140, 1449
MaeII ACGT 3 cut(s) 365, 951, 1398
MaeIII GTNAC 1 cut(s) 440
MalI GATC 8 cut(s) 14, 86, 165, 450, 459, 996, 1020, 1413
MboI GATC 8 cut(s) 12, 84, 163, 448, 457, 994, 1018, 1411
MboII GAAGA 3 cut(s) 856, 1373, 1376
MflI RGATCY 3 cut(s) 12, 84, 163
MhlI GDGCHC 1 cut(s) 40
MlyI GAGTC 2 cut(s) 247, 1036
Mph1103I ATGCAT 2 cut(s) 128, 857
MroI TCCGGA 1 cut(s) 225
MseI TTAA 3 cut(s) 291, 372, 1492
MslI CAYNNNNRTG 2 cut(s) 131, 594
MspA1I CMGCKG 1 cut(s) 348
MspI CCGG 2 cut(s) 226, 902
MspR9I CCNGG 2 cut(s) 756, 902
MvaI CCWGG 1 cut(s) 756
MwoI GCNNNNNNNGC 3 cut(s) 412, 605, 793
NciI CCSGG 1 cut(s) 902
NcoI CCATGG 1 cut(s) 255
NdeII GATC 8 cut(s) 12, 84, 163, 448, 457, 994, 1018, 1411
NlaIV GGNNCC 5 cut(s) 14, 120, 270, 1122, 1314
NmuCI GTSAC 1 cut(s) 440
NsiI ATGCAT 2 cut(s) 128, 857
PagI TCATGA 1 cut(s) 451
PfeI GAWTC 5 cut(s) 7, 229, 623, 665, 1028
PflMI CCANNNNNTGG 2 cut(s) 144, 840
PfoI TCCNGGA 1 cut(s) 900
PkrI GCNGC 3 cut(s) 476, 601, 1067
PleI GAGTC 2 cut(s) 246, 1036
PpsI GAGTC 2 cut(s) 246, 1036
PshBI ATTAAT 1 cut(s) 291
PsiI TTATAA 1 cut(s) 432
Psp6I CCWGG 1 cut(s) 754
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 5 cut(s) 14, 120, 270, 1122, 1314
PstI CTGCAG 1 cut(s) 1348
PsuI RGATCY 3 cut(s) 12, 84, 163
RsaI GTAC 6 cut(s) 364, 558, 1010, 1208, 1245, 1281
RsaNI GTAC 6 cut(s) 363, 557, 1009, 1207, 1244, 1280
RseI CAYNNNNRTG 2 cut(s) 131, 594
SaqAI TTAA 3 cut(s) 291, 372, 1492
SatI GCNGC 3 cut(s) 475, 600, 1066
Sau3AI GATC 8 cut(s) 12, 84, 163, 448, 457, 994, 1018, 1411
SchI GAGTC 2 cut(s) 247, 1036
ScrFI CCNGG 2 cut(s) 756, 902
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 1 cut(s) 167
SfcI CTRYAG 2 cut(s) 637, 1344
SmiMI CAYNNNNRTG 2 cut(s) 131, 594
SsiI CCGC 4 cut(s) 217, 348, 811, 1065
SspMI CTAG 4 cut(s) 285, 1032, 1140, 1449
StyD4I CCNGG 2 cut(s) 754, 900
StyI CCWWGG 2 cut(s) 255, 603
TaaI ACNGT 6 cut(s) 99, 440, 726, 871, 1117, 1437
TaiI ACGT 3 cut(s) 368, 954, 1401
TaqI TCGA 4 cut(s) 236, 424, 733, 944
TatI WGTACW 1 cut(s) 1206
TauI GCSGC 1 cut(s) 1068
TfiI GAWTC 5 cut(s) 7, 229, 623, 665, 1028
Tru1I TTAA 3 cut(s) 291, 372, 1492
Tru9I TTAA 3 cut(s) 291, 372, 1492
TscAI CASTG 2 cut(s) 731, 1314
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 2 cut(s) 474, 599
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 3 cut(s) 235, 663, 699
TspGWI ACGGA 3 cut(s) 658, 735, 933
TspRI CASTG 2 cut(s) 731, 1314
Van91I CCANNNNNTGG 2 cut(s) 144, 840
VspI ATTAAT 1 cut(s) 291
XapI RAATTY 2 cut(s) 58, 1475
XmiI GTMKAC 1 cut(s) 94
XspI CTAG 4 cut(s) 285, 1032, 1140, 1449
Zsp2I ATGCAT 2 cut(s) 128, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.