Rroxscaffold_4G00310850

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000947
Physical Location & Seq
Forward (+)
33020249 .. 33021568
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00310850.1

Sequence Viewer

Length: 1320 bp
ATGGTGAATCTATGCGAGGATTTGCTGGCTGAAATATTGCGTAGATTATCGGAGGAAGATCTGCTGAAATGTCAGAGCGTATCCAAGTCATGGCACCCAGTGATCTCACGTCTCTGGGTGCAGAGGTTCTGGACCCTGTCTTCATCGCCTGTTCTGGGTCTTTACTTCCGAACCACGACCGCCGGTGTGGAGCTTTCTCCTTACTTGCAAATGATGGACGGCGGTATGCCATCTCTTGTGAATTACATCTCTTTGTACGATCACAGTTTCCTGGAAGATAACCTCGAAAGAAATATGAAGAACACGCAGATCTTTCGACACTGGCAACGGGGAGTCGCTGAGGAATATCTTGACTGTTGCAATGGGTTACTTTTGATATATAAGTCCACCAGTCATCAGTTGTACGTCTGCAACCCTATCACCAGAAGACAATTTGCAATACCTAAAGCTTCCGATCACCACCGCAGTGGGCACTTCTGGGCAGCCTTGGCTTTCGACCCTTGTGAATCACCTAGCCACTATAGAGTTGTTCGAGTTGATTATAGTTGTTCCTTTCTCGACATATTTTCATCCCATTCTGGCCGGTGGGTTCGACATAAGCTTGACCCCGACTTCACTGAGCTTTGCCGCGGTCGTTTTGTATATCTACGAGGGATGTTATACAGCATAGCCAGCTCAGGTGAGGAGCTTTTATGCATTCACCTTGATACAACAGTTCAATCCAGGTCTTTTCAACTCCCTGAGTCTGTTAAGTGTCGAACCCACCGATGTCTAGGAGTGTCAATGGACCTTCTCTGTTATTTCTTCCGAGATGATGAGTCAAATATGTTTCATGTTTGGTCTTATGATGATCGCCAATCTTCAGATGAATGGGTTCTAAGATTTAGTATTTCCTGCGAAAAATTGAGATGGCTCATGTTAACTACTCTAGGCTATGACTACCCTGATACCCCTGAAACGTTTGAACCCTATGCAATTAGTCCAAGTTCTGATATTATCTTCTTTGGCACTCCCAGATTGATCTTAAGCTACAACTTTAATACCCTTAAGATCAAATTTGTTTGTAAAACAGGTTATGACATAGCTGCTTCACCTGTCTTTGCATTACGTGCTTGCTTTTTACCTCTCTACCAGTTGGGTAAGAGGAATGGTGGTTGTACTCCATGTGCAGATGATTCTTTGGGTGAGGAACTACCCTGTGATGAGGAACTAGAAGAAGAAGAAGACGGTGGTGAAATACAACTGTTCAACATCAAATTATTCTCTCCGGAGCAGAAAAGGTTAATTCTTCCTAGTGAACTGCATAGAATTATTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

439

Amino Acids

50.88

Weight (kDa)

5.57

Isoelectric Point (pI)

58.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 3 - 38 1.2e-06 F-box domain
F-box-like PF12937 6 - 41 7.9e-06 F-box-like
FBA_3 PF08268 112 - 196 8e-06 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 113 - 311 2.3e-07 KIB1-4 beta-propeller
b-prop_At3g26010-like PF24750 115 - 301 6.1e-11 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 119 - 282 6.8e-12 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 93
AccB7I CCANNNNNTGG 1 cut(s) 90
AccII CGCG 1 cut(s) 630
AccIII TCCGGA 1 cut(s) 1267
AciI CCGC 5 cut(s) 180, 222, 463, 628, 630
AclI AACGTT 1 cut(s) 959
AcoI YGGCCR 1 cut(s) 580
AcsI RAATTY 1 cut(s) 1055
AcuI CTGAAG 1 cut(s) 846
AdeI CACNNNGTG 1 cut(s) 100
AfaI GTAC 3 cut(s) 257, 404, 1159
AfiI CCNNNNNNNGG 2 cut(s) 90, 155
AflII CTTAAG 2 cut(s) 1024, 1046
AgsI TTSAA 4 cut(s) 719, 734, 965, 1249
AjiI CACGTC 1 cut(s) 110
AjnI CCWGG 2 cut(s) 270, 722
AleI CACNNNNGTG 1 cut(s) 465
AluBI AGCT 8 cut(s) 193, 449, 601, 622, 675, 688, 1029, 1085
AluI AGCT 8 cut(s) 193, 449, 601, 622, 675, 688, 1029, 1085
Alw26I GTCTC 1 cut(s) 116
Aor13HI TCCGGA 1 cut(s) 1267
AoxI GGCC 1 cut(s) 580
ApeKI GCWGC 2 cut(s) 482, 1085
ApoI RAATTY 1 cut(s) 1055
Asp700I GAANNNNTTC 1 cut(s) 873
AspS9I GGNCC 2 cut(s) 132, 787
AsuHPI GGTGA 9 cut(s) 16, 412, 449, 501, 692, 692, 1083, 1196, 1244
AvaII GGWCC 2 cut(s) 132, 787
BaeGI GKGCMC 1 cut(s) 474
BaeI ACNNNNGTAYC 2 cut(s) 699, 732
BanI GGYRCC 1 cut(s) 93
BbsI GAAGAC 3 cut(s) 132, 433, 1230
BbvCI CCTCAGC 1 cut(s) 339
BbvI GCAGC 2 cut(s) 494, 1072
BccI CCATC 3 cut(s) 208, 238, 903
BceAI ACGGC 1 cut(s) 235
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 2 cut(s) 272, 724
BciVI GTATCC 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 5 cut(s) 513, 773, 929, 1211, 1293
BfmI CTRYAG 1 cut(s) 520
BfrI CTTAAG 2 cut(s) 1024, 1046
BfuI GTATCC 1 cut(s) 91
BglII AGATCT 2 cut(s) 58, 309
BisI GCNGC 3 cut(s) 483, 628, 1086
BlsI GCNGC 3 cut(s) 484, 629, 1087
Bme1390I CCNGG 2 cut(s) 272, 724
Bme18I GGWCC 2 cut(s) 132, 787
BmgBI CACGTC 1 cut(s) 110
BmgT120I GGNCC 2 cut(s) 132, 787
BmiI GGNNCC 2 cut(s) 95, 134
BmrFI CCNGG 2 cut(s) 272, 724
BmrI ACTGGG 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 92
BpiI GAAGAC 3 cut(s) 132, 433, 1230
Bpu10I CCTNAGC 2 cut(s) 339, 676
BsaAI YACGTR 1 cut(s) 1109
BsaJI CCNNGG 2 cut(s) 486, 628
BsaWI WCCGGW 1 cut(s) 1267
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 155
Bse118I RCCGGY 2 cut(s) 182, 582
Bse1I ACTGG 4 cut(s) 98, 326, 390, 1132
Bse3DI GCAATG 1 cut(s) 367
BseAI TCCGGA 1 cut(s) 1267
BseBI CCWGG 2 cut(s) 272, 724
BseDI CCNNGG 2 cut(s) 486, 628
BseGI GGATG 2 cut(s) 569, 660
BseLI CCNNNNNNNGG 2 cut(s) 90, 155
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 4 cut(s) 330, 609, 690, 732
BseNI ACTGG 4 cut(s) 98, 326, 390, 1132
BseRI GAGGAG 1 cut(s) 698
BseSI GKGCMC 1 cut(s) 474
BseXI GCAGC 2 cut(s) 494, 1072
BsgI GTGCAG 2 cut(s) 140, 1188
Bsh1236I CGCG 1 cut(s) 630
Bsh1285I CGRYCG 2 cut(s) 180, 634
BshFI GGCC 1 cut(s) 582
BshNI GGYRCC 1 cut(s) 93
BsiEI CGRYCG 2 cut(s) 180, 634
BsiSI CCGG 3 cut(s) 183, 583, 1268
BslI CCNNNNNNNGG 2 cut(s) 90, 155
BsmAI GTCTC 1 cut(s) 116
BsmBI CGTCTC 1 cut(s) 116
BsmI GAATGC 1 cut(s) 696
BsnI GGCC 1 cut(s) 582
Bsp1286I GDGCHC 1 cut(s) 474
Bsp13I TCCGGA 1 cut(s) 1267
Bsp143I GATC 8 cut(s) 58, 102, 259, 309, 454, 850, 1020, 1050
BspACI CCGC 5 cut(s) 180, 222, 463, 628, 630
BspANI GGCC 1 cut(s) 582
BspCNI CTCAG 4 cut(s) 331, 610, 689, 733
BspEI TCCGGA 1 cut(s) 1267
BspFNI CGCG 1 cut(s) 630
BspLI GGNNCC 2 cut(s) 95, 134
BspT107I GGYRCC 1 cut(s) 93
BspTI CTTAAG 2 cut(s) 1024, 1046
BsrDI GCAATG 1 cut(s) 367
BsrFI RCCGGY 2 cut(s) 182, 582
BsrI ACTGG 4 cut(s) 98, 326, 390, 1132
BssAI RCCGGY 2 cut(s) 182, 582
BssECI CCNNGG 2 cut(s) 486, 628
BssMI GATC 8 cut(s) 58, 102, 259, 309, 454, 850, 1020, 1050
BssT1I CCWWGG 1 cut(s) 486
Bst2UI CCWGG 2 cut(s) 272, 724
Bst4CI ACNGT 5 cut(s) 266, 356, 715, 1229, 1245
BstAFI CTTAAG 2 cut(s) 1024, 1046
BstAPI GCANNNNNTGC 1 cut(s) 1109
BstBAI YACGTR 1 cut(s) 1109
BstC8I GCNNGC 3 cut(s) 27, 673, 1114
BstDEI CTNAG 5 cut(s) 339, 618, 676, 741, 878
BstDSI CCRYGG 1 cut(s) 628
BstF5I GGATG 2 cut(s) 569, 660
BstFNI CGCG 1 cut(s) 630
BstKTI GATC 8 cut(s) 61, 105, 262, 312, 457, 853, 1023, 1053
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 8 cut(s) 58, 102, 259, 309, 454, 850, 1020, 1050
BstMCI CGRYCG 2 cut(s) 180, 634
BstMWI GCNNNNNNNGC 3 cut(s) 488, 672, 1109
BstNI CCWGG 2 cut(s) 272, 724
BstSCI CCNGG 2 cut(s) 270, 722
BstSFI CTRYAG 1 cut(s) 520
BstSLI GKGCMC 1 cut(s) 474
BstUI CGCG 1 cut(s) 630
BstV1I GCAGC 2 cut(s) 494, 1072
BstV2I GAAGAC 3 cut(s) 132, 433, 1230
BstX2I RGATCY 2 cut(s) 58, 309
BstXI CCANNNNNNTGG 1 cut(s) 467
BstYI RGATCY 2 cut(s) 58, 309
BsuI GTATCC 1 cut(s) 91
BsuRI GGCC 1 cut(s) 582
BtgI CCRYGG 1 cut(s) 628
BtgZI GCGATG 1 cut(s) 129
BtrI CACGTC 1 cut(s) 110
BtsCI GGATG 2 cut(s) 569, 660
BtsI GCAGTG 1 cut(s) 472
BtsIMutI CAGTG 4 cut(s) 105, 319, 472, 615
Cac8I GCNNGC 3 cut(s) 27, 673, 1114
Cfr10I RCCGGY 2 cut(s) 182, 582
Cfr13I GGNCC 2 cut(s) 132, 787
Cfr42I CCGCGG 1 cut(s) 631
Csp6I GTAC 3 cut(s) 256, 403, 1158
CviAII CATG 4 cut(s) 90, 833, 916, 1164
CviQI GTAC 3 cut(s) 256, 403, 1158
DdeI CTNAG 5 cut(s) 339, 618, 676, 741, 878
DpnI GATC 8 cut(s) 60, 104, 261, 311, 456, 852, 1022, 1052
DpnII GATC 8 cut(s) 58, 102, 259, 309, 454, 850, 1020, 1050
DraIII CACNNNGTG 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 580
Eco130I CCWWGG 1 cut(s) 486
Eco47I GGWCC 2 cut(s) 132, 787
Eco57I CTGAAG 1 cut(s) 846
EcoRII CCWGG 2 cut(s) 270, 722
EcoT14I CCWWGG 1 cut(s) 486
EcoT22I ATGCAT 1 cut(s) 698
ErhI CCWWGG 1 cut(s) 486
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 4 cut(s) 93, 836, 919, 1167
FatI CATG 4 cut(s) 89, 832, 915, 1163
Fnu4HI GCNGC 3 cut(s) 483, 628, 1086
FokI GGATG 2 cut(s) 556, 667
Fsp4HI GCNGC 3 cut(s) 483, 628, 1086
FspBI CTAG 5 cut(s) 513, 773, 929, 1211, 1293
GluI GCNGC 3 cut(s) 483, 628, 1086
HaeIII GGCC 1 cut(s) 582
HapII CCGG 3 cut(s) 183, 583, 1268
Hin1II CATG 4 cut(s) 93, 836, 919, 1167
HincII GTYRAC 1 cut(s) 921
HindII GTYRAC 1 cut(s) 921
HindIII AAGCTT 2 cut(s) 447, 599
HinfI GANTC 6 cut(s) 7, 333, 506, 743, 818, 1175
HpaI GTTAAC 1 cut(s) 921
HpaII CCGG 3 cut(s) 183, 583, 1268
HphI GGTGA 9 cut(s) 16, 412, 449, 501, 692, 692, 1083, 1196, 1244
Hpy166II GTNNAC 3 cut(s) 387, 921, 1298
Hpy188I TCNGA 7 cut(s) 52, 75, 170, 454, 809, 865, 991
Hpy188III TCNNGA 4 cut(s) 130, 350, 557, 1268
Hpy8I GTNNAC 3 cut(s) 387, 921, 1298
HpyAV CCTTC 1 cut(s) 800
HpyCH4III ACNGT 5 cut(s) 266, 356, 715, 1229, 1245
HpyCH4IV ACGT 4 cut(s) 109, 405, 959, 1108
HpyF10VI GCNNNNNNNGC 3 cut(s) 488, 672, 1109
HpyF3I CTNAG 5 cut(s) 339, 618, 676, 741, 878
HpySE526I ACGT 4 cut(s) 109, 405, 959, 1108
Hsp92II CATG 4 cut(s) 93, 836, 919, 1167
Kpn2I TCCGGA 1 cut(s) 1267
KspAI GTTAAC 1 cut(s) 921
KspI CCGCGG 1 cut(s) 631
Kzo9I GATC 8 cut(s) 58, 102, 259, 309, 454, 850, 1020, 1050
LmnI GCTCC 3 cut(s) 190, 685, 1270
Lsp1109I GCAGC 2 cut(s) 494, 1072
MaeI CTAG 5 cut(s) 513, 773, 929, 1211, 1293
MaeII ACGT 4 cut(s) 109, 405, 959, 1108
MaeIII GTNAC 1 cut(s) 366
MalI GATC 8 cut(s) 60, 104, 261, 311, 456, 852, 1022, 1052
MboI GATC 8 cut(s) 58, 102, 259, 309, 454, 850, 1020, 1050
MflI RGATCY 2 cut(s) 58, 309
MhlI GDGCHC 1 cut(s) 474
MluCI AATT 8 cut(s) 241, 431, 902, 975, 1055, 1256, 1284, 1308
MlyI GAGTC 3 cut(s) 342, 752, 827
Mph1103I ATGCAT 1 cut(s) 698
MroI TCCGGA 1 cut(s) 1267
MroXI GAANNNNTTC 1 cut(s) 873
MseI TTAA 6 cut(s) 750, 920, 1025, 1038, 1047, 1283
MslI CAYNNNNRTG 1 cut(s) 465
MspA1I CMGCKG 1 cut(s) 630
MspCI CTTAAG 2 cut(s) 1024, 1046
MspI CCGG 3 cut(s) 183, 583, 1268
MspR9I CCNGG 2 cut(s) 272, 724
Mva1269I GAATGC 1 cut(s) 696
MvaI CCWGG 2 cut(s) 272, 724
MvnI CGCG 1 cut(s) 630
MwoI GCNNNNNNNGC 3 cut(s) 488, 672, 1109
NdeII GATC 8 cut(s) 58, 102, 259, 309, 454, 850, 1020, 1050
NlaIII CATG 4 cut(s) 93, 836, 919, 1167
NlaIV GGNNCC 2 cut(s) 95, 134
NsiI ATGCAT 1 cut(s) 698
OliI CACNNNNGTG 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 763
PctI GAATGC 1 cut(s) 696
PdmI GAANNNNTTC 1 cut(s) 873
PfeI GAWTC 3 cut(s) 7, 506, 1175
PflFI GACNNNGTC 1 cut(s) 136
PflMI CCANNNNNTGG 1 cut(s) 90
PfoI TCCNGGA 1 cut(s) 270
PkrI GCNGC 3 cut(s) 484, 629, 1087
PleI GAGTC 3 cut(s) 341, 751, 826
PpsI GAGTC 3 cut(s) 341, 751, 826
Ppu21I YACGTR 1 cut(s) 1109
Psp1406I AACGTT 1 cut(s) 959
Psp6I CCWGG 2 cut(s) 270, 722
PspGI CCWGG 2 cut(s) 270, 722
PspN4I GGNNCC 2 cut(s) 95, 134
PspPI GGNCC 2 cut(s) 132, 787
PsuI RGATCY 2 cut(s) 58, 309
PsyI GACNNNGTC 1 cut(s) 136
RsaI GTAC 3 cut(s) 257, 404, 1159
RsaNI GTAC 3 cut(s) 256, 403, 1158
RseI CAYNNNNRTG 1 cut(s) 465
SacII CCGCGG 1 cut(s) 631
SaqAI TTAA 6 cut(s) 750, 920, 1025, 1038, 1047, 1283
SatI GCNGC 3 cut(s) 483, 628, 1086
Sau3AI GATC 8 cut(s) 58, 102, 259, 309, 454, 850, 1020, 1050
Sau96I GGNCC 2 cut(s) 132, 787
SchI GAGTC 3 cut(s) 342, 752, 827
ScrFI CCNGG 2 cut(s) 272, 724
SduI GDGCHC 1 cut(s) 474
SfcI CTRYAG 1 cut(s) 520
Sfr303I CCGCGG 1 cut(s) 631
SgrAI CRCCGGYG 1 cut(s) 182
SgrBI CCGCGG 1 cut(s) 631
SinI GGWCC 2 cut(s) 132, 787
SmiMI CAYNNNNRTG 1 cut(s) 465
SmlI CTYRAG 2 cut(s) 1024, 1046
SmoI CTYRAG 2 cut(s) 1024, 1046
Sse9I AATT 8 cut(s) 241, 431, 902, 975, 1055, 1256, 1284, 1308
SsiI CCGC 5 cut(s) 180, 222, 463, 628, 630
SspI AATATT 1 cut(s) 36
SspMI CTAG 5 cut(s) 513, 773, 929, 1211, 1293
StyD4I CCNGG 2 cut(s) 270, 722
StyI CCWWGG 1 cut(s) 486
TaaI ACNGT 5 cut(s) 266, 356, 715, 1229, 1245
TaiI ACGT 4 cut(s) 112, 408, 962, 1111
TaqI TCGA 7 cut(s) 285, 316, 495, 532, 558, 592, 757
TasI AATT 8 cut(s) 241, 431, 902, 975, 1055, 1256, 1284, 1308
TatI WGTACW 1 cut(s) 1157
TauI GCSGC 1 cut(s) 630
TfiI GAWTC 3 cut(s) 7, 506, 1175
Tru1I TTAA 6 cut(s) 750, 920, 1025, 1038, 1047, 1283
Tru9I TTAA 6 cut(s) 750, 920, 1025, 1038, 1047, 1283
TscAI CASTG 4 cut(s) 105, 326, 472, 622
TseI GCWGC 2 cut(s) 482, 1085
TspDTI ATGAA 5 cut(s) 132, 311, 558, 821, 882
TspRI CASTG 4 cut(s) 105, 326, 472, 622
Tth111I GACNNNGTC 1 cut(s) 136
Van91I CCANNNNNTGG 1 cut(s) 90
Vha464I CTTAAG 2 cut(s) 1024, 1046
VpaK11BI GGWCC 2 cut(s) 132, 787
XapI RAATTY 1 cut(s) 1055
XmnI GAANNNNTTC 1 cut(s) 873
XspI CTAG 5 cut(s) 513, 773, 929, 1211, 1293
Zsp2I ATGCAT 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.