Glutathione S-transferase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 5 cut(s) | 16, 40, 262, 275, 599 |
| AcsI | RAATTY | 3 cut(s) | 366, 479, 520 |
| AcuI | CTGAAG | 2 cut(s) | 692, 700 |
| AfaI | GTAC | 3 cut(s) | 146, 223, 417 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 318 |
| AgsI | TTSAA | 7 cut(s) | 76, 380, 446, 464, 504, 509, 575 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 570, 602 |
| AluBI | AGCT | 6 cut(s) | 305, 362, 644, 673, 694, 701 |
| AluI | AGCT | 6 cut(s) | 305, 362, 644, 673, 694, 701 |
| Alw26I | GTCTC | 1 cut(s) | 226 |
| AlwI | GGATC | 5 cut(s) | 16, 40, 262, 275, 599 |
| Ama87I | CYCGRG | 1 cut(s) | 284 |
| AoxI | GGCC | 3 cut(s) | 69, 281, 393 |
| ApaI | GGGCCC | 1 cut(s) | 285 |
| ApeKI | GCWGC | 2 cut(s) | 694, 743 |
| ApoI | RAATTY | 3 cut(s) | 366, 479, 520 |
| AseI | ATTAAT | 1 cut(s) | 345 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 582 |
| AspS9I | GGNCC | 4 cut(s) | 247, 281, 282, 560 |
| AsuHPI | GGTGA | 3 cut(s) | 31, 649, 676 |
| AsuNHI | GCTAGC | 2 cut(s) | 301, 697 |
| AvaI | CYCGRG | 1 cut(s) | 284 |
| AvaII | GGWCC | 2 cut(s) | 247, 560 |
| AxyI | CCTNAGG | 1 cut(s) | 263 |
| BaeGI | GKGCMC | 1 cut(s) | 285 |
| BamHI | GGATCC | 1 cut(s) | 267 |
| BanII | GRGCYC | 2 cut(s) | 131, 285 |
| BbsI | GAAGAC | 1 cut(s) | 620 |
| BbvI | GCAGC | 2 cut(s) | 681, 730 |
| BccI | CCATC | 3 cut(s) | 401, 437, 485 |
| BcoDI | GTCTC | 1 cut(s) | 226 |
| BfaI | CTAG | 3 cut(s) | 251, 302, 698 |
| BglII | AGATCT | 1 cut(s) | 112 |
| BisI | GCNGC | 2 cut(s) | 695, 744 |
| BlsI | GCNGC | 2 cut(s) | 696, 745 |
| Bme18I | GGWCC | 2 cut(s) | 247, 560 |
| BmeT110I | CYCGRG | 1 cut(s) | 284 |
| BmgT120I | GGNCC | 4 cut(s) | 247, 281, 282, 560 |
| BmiI | GGNNCC | 2 cut(s) | 269, 283 |
| BmsI | GCATC | 2 cut(s) | 96, 504 |
| BmtI | GCTAGC | 2 cut(s) | 305, 701 |
| BpiI | GAAGAC | 1 cut(s) | 620 |
| BpuEI | CTTGAG | 2 cut(s) | 125, 238 |
| BsaJI | CCNNGG | 1 cut(s) | 390 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 318 |
| Bse1I | ACTGG | 2 cut(s) | 72, 249 |
| Bse21I | CCTNAGG | 1 cut(s) | 263 |
| Bse3DI | GCAATG | 1 cut(s) | 131 |
| BseDI | CCNNGG | 1 cut(s) | 390 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 318 |
| BseMI | GCAATG | 1 cut(s) | 131 |
| BseMII | CTCAG | 1 cut(s) | 254 |
| BseNI | ACTGG | 2 cut(s) | 72, 249 |
| BseRI | GAGGAG | 2 cut(s) | 128, 672 |
| BseSI | GKGCMC | 1 cut(s) | 285 |
| BseXI | GCAGC | 2 cut(s) | 681, 730 |
| BsgI | GTGCAG | 1 cut(s) | 382 |
| BshFI | GGCC | 3 cut(s) | 71, 283, 395 |
| BsiHKCI | CYCGRG | 1 cut(s) | 284 |
| BslFI | GGGAC | 1 cut(s) | 540 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 318 |
| BsmAI | GTCTC | 1 cut(s) | 226 |
| BsmFI | GGGAC | 1 cut(s) | 540 |
| BsnI | GGCC | 3 cut(s) | 71, 283, 395 |
| BsoBI | CYCGRG | 1 cut(s) | 284 |
| Bsp120I | GGGCCC | 1 cut(s) | 281 |
| Bsp1286I | GDGCHC | 2 cut(s) | 131, 285 |
| Bsp143I | GATC | 5 cut(s) | 21, 32, 112, 267, 604 |
| BspANI | GGCC | 3 cut(s) | 71, 283, 395 |
| BspCNI | CTCAG | 1 cut(s) | 255 |
| BspHI | TCATGA | 1 cut(s) | 685 |
| BspLI | GGNNCC | 2 cut(s) | 269, 283 |
| BspOI | GCTAGC | 2 cut(s) | 305, 701 |
| BspPI | GGATC | 5 cut(s) | 16, 40, 262, 275, 599 |
| BsrDI | GCAATG | 1 cut(s) | 131 |
| BsrI | ACTGG | 2 cut(s) | 72, 249 |
| BssECI | CCNNGG | 1 cut(s) | 390 |
| BssMI | GATC | 5 cut(s) | 21, 32, 112, 267, 604 |
| BssT1I | CCWWGG | 1 cut(s) | 390 |
| Bst6I | CTCTTC | 1 cut(s) | 459 |
| BstC8I | GCNNGC | 3 cut(s) | 303, 397, 699 |
| BstDEI | CTNAG | 3 cut(s) | 41, 263, 326 |
| BstKTI | GATC | 5 cut(s) | 24, 35, 115, 270, 607 |
| BstMAI | GTCTC | 1 cut(s) | 226 |
| BstMBI | GATC | 5 cut(s) | 21, 32, 112, 267, 604 |
| BstSLI | GKGCMC | 1 cut(s) | 285 |
| BstV1I | GCAGC | 2 cut(s) | 681, 730 |
| BstV2I | GAAGAC | 1 cut(s) | 620 |
| BstX2I | RGATCY | 3 cut(s) | 112, 267, 604 |
| BstYI | RGATCY | 3 cut(s) | 112, 267, 604 |
| Bsu36I | CCTNAGG | 1 cut(s) | 263 |
| BsuRI | GGCC | 3 cut(s) | 71, 283, 395 |
| BtgZI | GCGATG | 1 cut(s) | 481 |
| Cac8I | GCNNGC | 3 cut(s) | 303, 397, 699 |
| CciI | TCATGA | 1 cut(s) | 685 |
| Cfr13I | GGNCC | 4 cut(s) | 247, 281, 282, 560 |
| Csp6I | GTAC | 3 cut(s) | 145, 222, 416 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 42, 77 |
| CviAII | CATG | 3 cut(s) | 29, 236, 686 |
| CviQI | GTAC | 3 cut(s) | 145, 222, 416 |
| DdeI | CTNAG | 3 cut(s) | 41, 263, 326 |
| DpnI | GATC | 5 cut(s) | 23, 34, 114, 269, 606 |
| DpnII | GATC | 5 cut(s) | 21, 32, 112, 267, 604 |
| Eam1104I | CTCTTC | 1 cut(s) | 459 |
| EarI | CTCTTC | 1 cut(s) | 459 |
| Eco130I | CCWWGG | 1 cut(s) | 390 |
| Eco24I | GRGCYC | 2 cut(s) | 131, 285 |
| Eco47I | GGWCC | 2 cut(s) | 247, 560 |
| Eco57I | CTGAAG | 2 cut(s) | 692, 700 |
| Eco81I | CCTNAGG | 1 cut(s) | 263 |
| Eco88I | CYCGRG | 1 cut(s) | 284 |
| EcoO109I | RGGNCCY | 1 cut(s) | 281 |
| EcoT14I | CCWWGG | 1 cut(s) | 390 |
| EcoT38I | GRGCYC | 2 cut(s) | 131, 285 |
| ErhI | CCWWGG | 1 cut(s) | 390 |
| FaeI | CATG | 3 cut(s) | 32, 239, 689 |
| FaiI | YATR | 5 cut(s) | 30, 237, 567, 602, 687 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 570, 602 |
| FaqI | GGGAC | 1 cut(s) | 540 |
| FatI | CATG | 3 cut(s) | 28, 235, 685 |
| Fnu4HI | GCNGC | 2 cut(s) | 695, 744 |
| FriOI | GRGCYC | 2 cut(s) | 131, 285 |
| Fsp4HI | GCNGC | 2 cut(s) | 695, 744 |
| FspBI | CTAG | 3 cut(s) | 251, 302, 698 |
| GluI | GCNGC | 2 cut(s) | 695, 744 |
| HaeIII | GGCC | 3 cut(s) | 71, 283, 395 |
| Hin1II | CATG | 3 cut(s) | 32, 239, 689 |
| HinfI | GANTC | 1 cut(s) | 202 |
| HphI | GGTGA | 3 cut(s) | 31, 649, 676 |
| Hpy166II | GTNNAC | 3 cut(s) | 157, 181, 312 |
| Hpy188I | TCNGA | 3 cut(s) | 117, 406, 719 |
| Hpy188III | TCNNGA | 5 cut(s) | 217, 533, 575, 608, 686 |
| Hpy8I | GTNNAC | 3 cut(s) | 157, 181, 312 |
| HpyAV | CCTTC | 2 cut(s) | 35, 469 |
| HpyCH4V | TGCA | 1 cut(s) | 399 |
| HpyF3I | CTNAG | 3 cut(s) | 41, 263, 326 |
| Hsp92II | CATG | 3 cut(s) | 32, 239, 689 |
| Kzo9I | GATC | 5 cut(s) | 21, 32, 112, 267, 604 |
| LmnI | GCTCC | 1 cut(s) | 141 |
| Lsp1109I | GCAGC | 2 cut(s) | 681, 730 |
| LweI | GCATC | 2 cut(s) | 96, 504 |
| MaeI | CTAG | 3 cut(s) | 251, 302, 698 |
| MaeIII | GTNAC | 2 cut(s) | 203, 637 |
| MalI | GATC | 5 cut(s) | 23, 34, 114, 269, 606 |
| MboI | GATC | 5 cut(s) | 21, 32, 112, 267, 604 |
| MboII | GAAGA | 7 cut(s) | 119, 122, 476, 590, 625, 698, 706 |
| MfeI | CAATTG | 1 cut(s) | 504 |
| MflI | RGATCY | 3 cut(s) | 112, 267, 604 |
| MhlI | GDGCHC | 2 cut(s) | 131, 285 |
| MluCI | AATT | 7 cut(s) | 342, 366, 449, 479, 504, 520, 597 |
| MlyI | GAGTC | 1 cut(s) | 211 |
| MnlI | CCTC | 7 cut(s) | 149, 258, 460, 573, 690, 693, 740 |
| MroXI | GAANNNNTTC | 1 cut(s) | 582 |
| MseI | TTAA | 2 cut(s) | 345, 452 |
| MunI | CAATTG | 1 cut(s) | 504 |
| NdeII | GATC | 5 cut(s) | 21, 32, 112, 267, 604 |
| NheI | GCTAGC | 2 cut(s) | 301, 697 |
| NlaIII | CATG | 3 cut(s) | 32, 239, 689 |
| NlaIV | GGNNCC | 2 cut(s) | 269, 283 |
| NmuCI | GTSAC | 2 cut(s) | 203, 637 |
| PagI | TCATGA | 1 cut(s) | 685 |
| PdmI | GAANNNNTTC | 1 cut(s) | 582 |
| PkrI | GCNGC | 2 cut(s) | 696, 745 |
| PleI | GAGTC | 1 cut(s) | 210 |
| PpsI | GAGTC | 1 cut(s) | 210 |
| PshBI | ATTAAT | 1 cut(s) | 345 |
| PspN4I | GGNNCC | 2 cut(s) | 269, 283 |
| PspOMI | GGGCCC | 1 cut(s) | 281 |
| PspPI | GGNCC | 4 cut(s) | 247, 281, 282, 560 |
| PsuI | RGATCY | 3 cut(s) | 112, 267, 604 |
| RsaI | GTAC | 3 cut(s) | 146, 223, 417 |
| RsaNI | GTAC | 3 cut(s) | 145, 222, 416 |
| SaqAI | TTAA | 2 cut(s) | 345, 452 |
| SatI | GCNGC | 2 cut(s) | 695, 744 |
| Sau3AI | GATC | 5 cut(s) | 21, 32, 112, 267, 604 |
| Sau96I | GGNCC | 4 cut(s) | 247, 281, 282, 560 |
| SchI | GAGTC | 1 cut(s) | 211 |
| SduI | GDGCHC | 2 cut(s) | 131, 285 |
| SfaNI | GCATC | 2 cut(s) | 96, 504 |
| SinI | GGWCC | 2 cut(s) | 247, 560 |
| SmlI | CTYRAG | 2 cut(s) | 140, 217 |
| SmoI | CTYRAG | 2 cut(s) | 140, 217 |
| Sse9I | AATT | 7 cut(s) | 342, 366, 449, 479, 504, 520, 597 |
| SspI | AATATT | 1 cut(s) | 627 |
| SspMI | CTAG | 3 cut(s) | 251, 302, 698 |
| StyI | CCWWGG | 1 cut(s) | 390 |
| TasI | AATT | 7 cut(s) | 342, 366, 449, 479, 504, 520, 597 |
| TatI | WGTACW | 3 cut(s) | 144, 221, 415 |
| Tru1I | TTAA | 2 cut(s) | 345, 452 |
| Tru9I | TTAA | 2 cut(s) | 345, 452 |
| TseFI | GTSAC | 2 cut(s) | 203, 637 |
| TseI | GCWGC | 2 cut(s) | 694, 743 |
| Tsp45I | GTSAC | 2 cut(s) | 203, 637 |
| TspDTI | ATGAA | 3 cut(s) | 17, 488, 702 |
| VpaK11BI | GGWCC | 2 cut(s) | 247, 560 |
| VspI | ATTAAT | 1 cut(s) | 345 |
| XapI | RAATTY | 3 cut(s) | 366, 479, 520 |
| XmnI | GAANNNNTTC | 1 cut(s) | 582 |
| XspI | CTAG | 3 cut(s) | 251, 302, 698 |