pycom01g00360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001044
Physical Location & Seq
Forward (+)
459678 .. 461605
1928 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g00360.2

Sequence Viewer

Length: 1560 bp
ATGCATGTGGTGGGACTAATACCTTTGATATCGGCCAACGTCCAACCTAGGGCAGATTTGAACTCCTTCAAAACACGCATTAGTTTCTCCTCTTCTTGTGCCGTGAGTGATGAAGAAATAATGGCCGGTAGTGTCTCCTCTTCCCCCAAGAAAATGTACTTCAAATGGGATGGGAGCAACTTATTAGTTGAAATGGGAATTGACTCAAAGTTAGAAAACTTACCCTCGTGCTTAGGCAATGACTCAAGGGCAGCCACCAACTCCAAGCATTCCTCACTAAAAAGCACGGCATGCAATGGAGTTTGTGTGCCGTGGGTGATGAAAGGTTCTACTCCCTTGGTTTTGACTTCCATGCTTCGTGTAATGACATTTTCTAGTGCATCGGCGTTCAAATCTTCAAAATATCTCTGCGCCAATGAACTTAATAGAATCAGAAAGACTAAAATTAACAACTTCCCCATCAAATTCCATGGACAAGGTTCCATTAAACACATATATTTTCGTCTGAGCCGTCTTCATGAATGGCCGTCCAAGAAGGATTGGCAACGTAGGGGCATGGTCAGATTCATCCATCTCGACTTCCAAAACTCCCTTTGGATAGGCATTAGATCTATCGGCCAATTTGAATATGGCATAACATTTATAGAAGCACCTAGGTCTAGCATGGCAGATTCAAATTTAGTGTTTCCAATGACACAAGGAATGGTAAAACTACCCGGATCTTTGCATTTGGGGGGTAGTTTGCGTTGCAAGATGGCGGAGACATTTTCACCTACCTTTACCACCTCTTTGGTCGACATCCTCTTCCTAGTGGTGCAAAGCTCCTTTAGAAACTTGGTTCACTTGAACTTTCCGAAACGTCTCAAGGATGTCCTTCTCGGCTTCTTTTTTTGCGATTGCATAAACCTACTAGGAAAAGGCACATTCGGAGGAAAAACATTAGTAGGAACCGAATTTGACACATTCTTACCCTTTTTGGCCGAATTGGACATTTTTGGATCCACAAAAGCTTGCGGCAAAGGTGTTTCCACCTTTGCCGTGGATTGGCCTATTTCCTCCTCTTCAATCCTCAGTTTTTCTTCCTCGTTAAGACTTGATGGTGATGGTGTAGGACCTGCCCCGACTTCTTTTGCGCTTCTCAAGAGTATGGCCTTTGCACTTTCGAAACCTCCATTGGGGTTTGGAATGGTAGAACTAGGAACTGCCCAATTTGTTTCTCCAATTGCTCAACCCTCTTGTCTTGGTTTTGCATGGCTTTGGCTTGGTTTTCTTGCCCCTGAGACAAATTCATTGTGGAGGTGCTTGTGTGGGGACGAATGGCTTGTTGTAGAAGCCCGGGGTTTGTTGCCTAAAGCCTCCTTGTTGTGGTGGTTGTTGGGGCTCCCTCCATTTGAAATTCGGGTGGTCTCTCCAACCTGGATTATACGTGTTCGAATATGGATCGTTCCTCGGTTGCCACCATTTTCAATGAGTTGTGGGCACTTCTCAGAAGCATGTCCTTGGATAGAACACACTCCGCATACAATTGGTCCTTGCACCCTCATTCCTTCGGCCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

520

Amino Acids

57.29

Weight (kDa)

9.23

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1123
AccI GTMKAC 1 cut(s) 795
AciI CCGC 3 cut(s) 758, 1014, 1517
AclWI GGATC 4 cut(s) 727, 993, 1006, 1448
AcoI YGGCCR 6 cut(s) 33, 123, 524, 616, 978, 1551
AcsI RAATTY 5 cut(s) 464, 676, 953, 1285, 1395
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 4 cut(s) 49, 1044, 1175, 1365
AflIII ACRYGT 1 cut(s) 1426
AjnI CCWGG 1 cut(s) 1415
AjuI GAANNNNNNNTTGG 2 cut(s) 1157, 1189
AluBI AGCT 2 cut(s) 822, 1010
AluI AGCT 2 cut(s) 822, 1010
Alw26I GTCTC 5 cut(s) 139, 755, 866, 1274, 1411
AlwI GGATC 4 cut(s) 727, 993, 1006, 1448
Ama87I CYCGRG 1 cut(s) 1335
AoxI GGCC 8 cut(s) 33, 123, 524, 616, 978, 1046, 1149, 1551
ApeKI GCWGC 1 cut(s) 251
ApoI RAATTY 5 cut(s) 464, 676, 953, 1285, 1395
Asp700I GAANNNNTTC 1 cut(s) 65
AspA2I CCTAGG 2 cut(s) 47, 653
AspLEI GCGC 2 cut(s) 413, 1135
AspS9I GGNCC 2 cut(s) 1112, 1529
AsuC2I CCSGG 3 cut(s) 717, 1336, 1337
AsuHPI GGTGA 3 cut(s) 328, 762, 1112
AsuII TTCGAA 2 cut(s) 1163, 1432
AvaI CYCGRG 1 cut(s) 1335
AvaII GGWCC 2 cut(s) 1112, 1529
AvrII CCTAGG 2 cut(s) 47, 653
BaeGI GKGCMC 1 cut(s) 1482
BamHI GGATCC 1 cut(s) 998
BanII GRGCYC 1 cut(s) 1383
BauI CACGAG 1 cut(s) 226
BbsI GAAGAC 1 cut(s) 506
BbvI GCAGC 1 cut(s) 263
BccI CCATC 6 cut(s) 164, 467, 579, 748, 1091, 1097
BceAI ACGGC 6 cut(s) 86, 295, 303, 495, 511, 1022
BciT130I CCWGG 1 cut(s) 1417
BcnI CCSGG 3 cut(s) 717, 1336, 1337
BcoDI GTCTC 5 cut(s) 139, 755, 866, 1274, 1411
BfaI CTAG 7 cut(s) 48, 375, 654, 660, 809, 911, 1196
BfuAI ACCTGC 1 cut(s) 1123
BglII AGATCT 1 cut(s) 608
BisI GCNGC 2 cut(s) 252, 1015
BlnI CCTAGG 2 cut(s) 47, 653
BlsI GCNGC 2 cut(s) 253, 1016
Bme1390I CCNGG 4 cut(s) 717, 1336, 1337, 1417
Bme18I GGWCC 2 cut(s) 1112, 1529
BmeT110I CYCGRG 1 cut(s) 1335
BmgT120I GGNCC 2 cut(s) 1112, 1529
BmiI GGNNCC 4 cut(s) 481, 949, 1000, 1382
BmrFI CCNGG 4 cut(s) 717, 1336, 1337, 1417
BmsI GCATC 1 cut(s) 389
BpiI GAAGAC 1 cut(s) 506
Bpu10I CCTNAGC 1 cut(s) 232
Bpu14I TTCGAA 2 cut(s) 1163, 1432
BpuEI CTTGAG 3 cut(s) 229, 848, 1124
BpuMI CCSGG 3 cut(s) 717, 1336, 1337
BsaAI YACGTR 1 cut(s) 1427
BsaI GGTCTC 1 cut(s) 1411
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 1044, 1175, 1365
Bse118I RCCGGY 1 cut(s) 125
Bse3DI GCAATG 2 cut(s) 244, 301
BseBI CCWGG 1 cut(s) 1417
BseGI GGATG 4 cut(s) 175, 567, 798, 874
BseLI CCNNNNNNNGG 4 cut(s) 49, 1044, 1175, 1365
BseMI GCAATG 2 cut(s) 244, 301
BseMII CTCAG 4 cut(s) 497, 1084, 1269, 1500
BseRI GAGGAG 3 cut(s) 79, 127, 1048
BseSI GKGCMC 1 cut(s) 1482
BseXI GCAGC 1 cut(s) 263
BshFI GGCC 8 cut(s) 35, 125, 526, 618, 980, 1048, 1151, 1553
BsiHKCI CYCGRG 1 cut(s) 1335
BsiSI CCGG 3 cut(s) 126, 717, 1336
BslFI GGGAC 2 cut(s) 27, 1325
BslI CCNNNNNNNGG 4 cut(s) 49, 1044, 1175, 1365
BsmAI GTCTC 5 cut(s) 139, 755, 866, 1274, 1411
BsmBI CGTCTC 1 cut(s) 866
BsmFI GGGAC 2 cut(s) 27, 1325
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 8 cut(s) 35, 125, 526, 618, 980, 1048, 1151, 1553
Bso31I GGTCTC 1 cut(s) 1411
BsoBI CYCGRG 1 cut(s) 1335
Bsp119I TTCGAA 2 cut(s) 1163, 1432
Bsp1286I GDGCHC 2 cut(s) 1383, 1482
Bsp143I GATC 4 cut(s) 608, 719, 998, 1440
Bsp19I CCATGG 1 cut(s) 469
BspACI CCGC 3 cut(s) 758, 1014, 1517
BspANI GGCC 8 cut(s) 35, 125, 526, 618, 980, 1048, 1151, 1553
BspCNI CTCAG 4 cut(s) 498, 1083, 1270, 1499
BspHI TCATGA 1 cut(s) 517
BspLI GGNNCC 4 cut(s) 481, 949, 1000, 1382
BspMI ACCTGC 1 cut(s) 1123
BspPI GGATC 4 cut(s) 727, 993, 1006, 1448
BspT104I TTCGAA 2 cut(s) 1163, 1432
BspTNI GGTCTC 1 cut(s) 1411
BsrDI GCAATG 2 cut(s) 244, 301
BsrFI RCCGGY 1 cut(s) 125
BssAI RCCGGY 1 cut(s) 125
BssMI GATC 4 cut(s) 608, 719, 998, 1440
BssSI CACGAG 1 cut(s) 226
BssT1I CCWWGG 5 cut(s) 47, 336, 469, 653, 1499
Bst2BI CACGAG 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 1417
Bst6I CTCTTC 4 cut(s) 97, 145, 809, 1066
BstAPI GCANNNNNTGC 1 cut(s) 291
BstBAI YACGTR 1 cut(s) 1427
BstBI TTCGAA 2 cut(s) 1163, 1432
BstC8I GCNNGC 2 cut(s) 292, 1012
BstDEI CTNAG 5 cut(s) 232, 506, 1070, 1278, 1486
BstDSI CCRYGG 3 cut(s) 311, 469, 1038
BstF5I GGATG 4 cut(s) 175, 567, 798, 874
BstHHI GCGC 2 cut(s) 413, 1135
BstKTI GATC 4 cut(s) 611, 722, 1001, 1443
BstMAI GTCTC 5 cut(s) 139, 755, 866, 1274, 1411
BstMBI GATC 4 cut(s) 608, 719, 998, 1440
BstMWI GCNNNNNNNGC 1 cut(s) 291
BstNI CCWGG 1 cut(s) 1417
BstNSI RCATGY 3 cut(s) 8, 294, 1497
BstSCI CCNGG 4 cut(s) 715, 1334, 1335, 1415
BstSLI GKGCMC 1 cut(s) 1482
BstV1I GCAGC 1 cut(s) 263
BstV2I GAAGAC 1 cut(s) 506
BstX2I RGATCY 3 cut(s) 608, 719, 998
BstXI CCANNNNNNTGG 1 cut(s) 790
BstYI RGATCY 3 cut(s) 608, 719, 998
BsuRI GGCC 8 cut(s) 35, 125, 526, 618, 980, 1048, 1151, 1553
BtgI CCRYGG 3 cut(s) 311, 469, 1038
BtsCI GGATG 4 cut(s) 175, 567, 798, 874
BveI ACCTGC 1 cut(s) 1123
Cac8I GCNNGC 2 cut(s) 292, 1012
CciI TCATGA 1 cut(s) 517
CfoI GCGC 2 cut(s) 413, 1135
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 1112, 1529
Cfr9I CCCGGG 1 cut(s) 1335
Csp6I GTAC 1 cut(s) 157
CviAII CATG 9 cut(s) 5, 291, 352, 470, 518, 556, 664, 1251, 1494
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 5 cut(s) 232, 506, 1070, 1278, 1486
DpnI GATC 4 cut(s) 610, 721, 1000, 1442
DpnII GATC 4 cut(s) 608, 719, 998, 1440
EaeI YGGCCR 6 cut(s) 33, 123, 524, 616, 978, 1551
Eam1104I CTCTTC 4 cut(s) 97, 145, 809, 1066
EarI CTCTTC 4 cut(s) 97, 145, 809, 1066
EciI GGCGGA 1 cut(s) 773
Eco130I CCWWGG 5 cut(s) 47, 336, 469, 653, 1499
Eco24I GRGCYC 1 cut(s) 1383
Eco31I GGTCTC 1 cut(s) 1411
Eco32I GATATC 1 cut(s) 30
Eco47I GGWCC 2 cut(s) 1112, 1529
Eco88I CYCGRG 1 cut(s) 1335
EcoO109I RGGNCCY 1 cut(s) 1112
EcoRII CCWGG 1 cut(s) 1415
EcoRV GATATC 1 cut(s) 30
EcoT14I CCWWGG 5 cut(s) 47, 336, 469, 653, 1499
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 1383
ErhI CCWWGG 5 cut(s) 47, 336, 469, 653, 1499
Esp3I CGTCTC 1 cut(s) 866
FaeI CATG 9 cut(s) 8, 294, 355, 473, 521, 559, 667, 1254, 1497
FaqI GGGAC 2 cut(s) 27, 1325
FatI CATG 9 cut(s) 4, 290, 351, 469, 517, 555, 663, 1250, 1493
FblI GTMKAC 1 cut(s) 795
Fnu4HI GCNGC 2 cut(s) 252, 1015
FokI GGATG 4 cut(s) 182, 554, 785, 881
FriOI GRGCYC 1 cut(s) 1383
Fsp4HI GCNGC 2 cut(s) 252, 1015
FspBI CTAG 7 cut(s) 48, 375, 654, 660, 809, 911, 1196
GlaI GCGC 2 cut(s) 412, 1134
GluI GCNGC 2 cut(s) 252, 1015
HaeIII GGCC 8 cut(s) 35, 125, 526, 618, 980, 1048, 1151, 1553
HapII CCGG 3 cut(s) 126, 717, 1336
HhaI GCGC 2 cut(s) 413, 1135
Hin1II CATG 9 cut(s) 8, 294, 355, 473, 521, 559, 667, 1254, 1497
Hin6I GCGC 2 cut(s) 411, 1133
HinP1I GCGC 2 cut(s) 411, 1133
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HindIII AAGCTT 1 cut(s) 1008
HinfI GANTC 5 cut(s) 203, 242, 429, 564, 671
HpaII CCGG 3 cut(s) 126, 717, 1336
HphI GGTGA 3 cut(s) 328, 762, 1112
Hpy166II GTNNAC 2 cut(s) 796, 841
Hpy188I TCNGA 7 cut(s) 434, 507, 563, 855, 929, 1489, 1559
Hpy188III TCNNGA 3 cut(s) 518, 575, 1141
Hpy8I GTNNAC 2 cut(s) 796, 841
HpyAV CCTTC 4 cut(s) 76, 529, 884, 1557
HpyCH4IV ACGT 4 cut(s) 39, 547, 859, 1426
HpyF10VI GCNNNNNNNGC 1 cut(s) 291
HpyF3I CTNAG 5 cut(s) 232, 506, 1070, 1278, 1486
HpySE526I ACGT 4 cut(s) 39, 547, 859, 1426
Hsp92II CATG 9 cut(s) 8, 294, 355, 473, 521, 559, 667, 1254, 1497
HspAI GCGC 2 cut(s) 411, 1133
Kzo9I GATC 4 cut(s) 608, 719, 998, 1440
LmnI GCTCC 3 cut(s) 174, 827, 1386
LpnPI CCDG 7 cut(s) 139, 730, 1128, 1290, 1349, 1402, 1429
Lsp1109I GCAGC 1 cut(s) 263
LweI GCATC 1 cut(s) 389
MaeI CTAG 7 cut(s) 48, 375, 654, 660, 809, 911, 1196
MaeII ACGT 4 cut(s) 39, 547, 859, 1426
MalI GATC 4 cut(s) 610, 721, 1000, 1442
MboI GATC 4 cut(s) 608, 719, 998, 1440
MboII GAAGA 8 cut(s) 84, 125, 132, 387, 506, 796, 1053, 1071
MfeI CAATTG 2 cut(s) 1221, 1524
MflI RGATCY 3 cut(s) 608, 719, 998
MhlI GDGCHC 2 cut(s) 1383, 1482
MlyI GAGTC 2 cut(s) 197, 236
MmeI TCCRAC 2 cut(s) 67, 1436
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 65
MseI TTAA 4 cut(s) 423, 447, 486, 1088
MspI CCGG 3 cut(s) 126, 717, 1336
MspR9I CCNGG 4 cut(s) 717, 1336, 1337, 1417
MunI CAATTG 2 cut(s) 1221, 1524
Mva1269I GAATGC 1 cut(s) 268
MvaI CCWGG 1 cut(s) 1417
MwoI GCNNNNNNNGC 1 cut(s) 291
NciI CCSGG 3 cut(s) 717, 1336, 1337
NcoI CCATGG 1 cut(s) 469
NdeII GATC 4 cut(s) 608, 719, 998, 1440
NlaIII CATG 9 cut(s) 8, 294, 355, 473, 521, 559, 667, 1254, 1497
NlaIV GGNNCC 4 cut(s) 481, 949, 1000, 1382
NmeAIII GCCGAG 1 cut(s) 858
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 8, 294, 1497
NspV TTCGAA 2 cut(s) 1163, 1432
PaeI GCATGC 1 cut(s) 294
PagI TCATGA 1 cut(s) 517
PcsI WCGNNNNNNNCGW 1 cut(s) 508
PctI GAATGC 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 65
PfeI GAWTC 3 cut(s) 429, 564, 671
PkrI GCNGC 2 cut(s) 253, 1016
PleI GAGTC 2 cut(s) 197, 236
PpsI GAGTC 2 cut(s) 197, 236
Ppu21I YACGTR 1 cut(s) 1427
PpuMI RGGWCCY 1 cut(s) 1112
Psp5II RGGWCCY 1 cut(s) 1112
Psp6I CCWGG 1 cut(s) 1415
PspGI CCWGG 1 cut(s) 1415
PspN4I GGNNCC 4 cut(s) 481, 949, 1000, 1382
PspPI GGNCC 2 cut(s) 1112, 1529
PspPPI RGGWCCY 1 cut(s) 1112
PsuI RGATCY 3 cut(s) 608, 719, 998
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
SalI GTCGAC 1 cut(s) 794
SaqAI TTAA 4 cut(s) 423, 447, 486, 1088
SatI GCNGC 2 cut(s) 252, 1015
Sau3AI GATC 4 cut(s) 608, 719, 998, 1440
Sau96I GGNCC 2 cut(s) 1112, 1529
SchI GAGTC 2 cut(s) 197, 236
ScrFI CCNGG 4 cut(s) 717, 1336, 1337, 1417
SduI GDGCHC 2 cut(s) 1383, 1482
SfaNI GCATC 1 cut(s) 389
SfuI TTCGAA 2 cut(s) 1163, 1432
SinI GGWCC 2 cut(s) 1112, 1529
SmaI CCCGGG 1 cut(s) 1337
SmlI CTYRAG 3 cut(s) 244, 863, 1139
SmoI CTYRAG 3 cut(s) 244, 863, 1139
SphI GCATGC 1 cut(s) 294
SsiI CCGC 3 cut(s) 758, 1014, 1517
SspMI CTAG 7 cut(s) 48, 375, 654, 660, 809, 911, 1196
StyD4I CCNGG 4 cut(s) 715, 1334, 1335, 1415
StyI CCWWGG 5 cut(s) 47, 336, 469, 653, 1499
TaiI ACGT 4 cut(s) 42, 550, 862, 1429
TaqI TCGA 4 cut(s) 576, 795, 1163, 1432
TatI WGTACW 1 cut(s) 156
TauI GCSGC 1 cut(s) 1017
TfiI GAWTC 3 cut(s) 429, 564, 671
Tru1I TTAA 4 cut(s) 423, 447, 486, 1088
Tru9I TTAA 4 cut(s) 423, 447, 486, 1088
TseI GCWGC 1 cut(s) 251
TspDTI ATGAA 7 cut(s) 126, 335, 432, 506, 534, 556, 1278
TspMI CCCGGG 1 cut(s) 1335
VpaK11BI GGWCC 2 cut(s) 1112, 1529
XapI RAATTY 5 cut(s) 464, 676, 953, 1285, 1395
XceI RCATGY 3 cut(s) 8, 294, 1497
XcmI CCANNNNNNNNNTGG 2 cut(s) 626, 1036
XmaI CCCGGG 1 cut(s) 1335
XmaJI CCTAGG 2 cut(s) 47, 653
XmiI GTMKAC 1 cut(s) 795
XmnI GAANNNNTTC 1 cut(s) 65
XspI CTAG 7 cut(s) 48, 375, 654, 660, 809, 911, 1196
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.