pycom1654g00130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00001654
OG0001044
Physical Location & Seq
Forward (+)
104960 .. 106871
1912 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom1654g00130.2

Sequence Viewer

Length: 1554 bp
ATGGCAGCCCTTTCAACAATGTTACTATGGCTACTTTCGGCCATATTGGGTGGTTGTAGACCAATCACTCCAAGTTCTTCTCCAATGATGGTGCTTGATACATCCTTCGTGGTAAGTGTGGATCGATCTTTCAGCCCTTTGTCCTTATGCACATCTACGACAAAGCAAGAACGGCTATCGTTAGGATTCTTAATAGATGTTAGTGCTCCTTTGGCCACATCAATCTTTGTTTGGGCCGTTTTCATGAACGGACGGCCTAGCAAGAGTGGCAATGATGGAGCATCCCTCAAGGACTCCCTTGGGATAAACATTAGAACGATCGGCCAATTGAATTATAACGCCATCTTGACATTAATAGAAGCACCTAAATCTAACATGCATTGTTTAAATCTAGTGTTTCCAATTGTGCATGGGATAGTAAAACTCCCTGGATCCTTACATTTTGGAGGTAACTTTCGTTGCAAAACCGCAGATACATTTTCACTTACTTTCACAACTTCTTTGTTTGAAATTCTTTTCCTAGTTGTGCATAACTCTTTTAAAAACTTTGCATACCTGGGCACTTGCTTAATGGCATCAAGTAAAGGAATGTTTACTTGGACTTTCCGAAACGTCTCCAGAATGTCTTTTTCCTGCTCCTCCTTCTTTGTTTGCATAAACCTGCTAGGAAAAGGAACATCGGGTGGAACAAAGTTAGAATTAATCAATTTTGAACCCAACTTACCTTTGGTGGCCGAATGGGATGAGTTGGGTGCATTAATGTCCCTAGGTAGTTGCGGCAAGGGTGTTTGTACCCTTGCCGTGGGCTTGCTTGCCTCCTCTTCTTCATGTTGCAGCTTTTCATCCTCTTCTTCGCTTGATTTTGGTGGTTGTGGGGTGGTTCCAACTTCCTTGCCACTTCTCAAGGTAATTACGTTGGCGGTTTCAAAGCCTCCCTTCGGGTTTGGAATGGTTGAGCTCGGGAGCTTGCCTTGGTCTCGGAATTGCCCTACAAACTCCGCAATCTGCCCAATTTACAAATTAGTTAGTAACTTAAGAAGTGTATCATTATCTAAGGTGTTACCTGAACTTGTTTGGGCAGATTGTGGAGGTGCTTGTGTGGGTGCGAATGGCTTGGTGAAGAAGCCCGGGGGTTGTTGCTTAAAGCCTCCTTGTTGTGGTGGTTGTTGGGGCTCCCTCCATTTGAAGTTCGGCGTTCCTTGGTTGGTTTTGGCCCTGAAATCCAATTGCATTGGCGCTTTCCCATCCACCATTTCCTATGAGTTGTGGGCACTTCTCAGAAGCATGTCCTTGGATCGAACACACCCCGCATACAATCGGTCCTTGCACCCTCATTCCTTCGATCGGGTGTGGAACTTACCTCATGCACTTGCTGCCGTGGGGGTCCTCTTTGGCCTACTCCTTCGTATTCCATGGGCGTCTTGTCCACCAATGCTCCACCCGCCGAGGCATCGAGCATTTGACGTTCGATTGGTAGGAGCCCCTCGTAGAAGTATTGCAAAAGTAACTCCTCCTTCATCTGATGCTGTGGACAAGACGCAACAAGAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

518

Amino Acids

55.43

Weight (kDa)

9.11

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 336
Acc36I ACCTGC 1 cut(s) 669
AccI GTMKAC 1 cut(s) 58
AciI CCGC 6 cut(s) 468, 777, 920, 999, 1308, 1442
AclWI GGATC 4 cut(s) 129, 426, 439, 1302
AcoI YGGCCR 4 cut(s) 39, 213, 322, 732
AcsI RAATTY 1 cut(s) 510
AcyI GRCGYC 1 cut(s) 1418
AfaI GTAC 1 cut(s) 793
AfiI CCNNNNNNNGG 4 cut(s) 802, 938, 1157, 1344
AflII CTTAAG 1 cut(s) 1033
AgsI TTSAA 6 cut(s) 15, 331, 509, 713, 927, 1186
AjnI CCWGG 2 cut(s) 427, 555
AjuI GAANNNNNNNTTGG 2 cut(s) 580, 612
AluBI AGCT 3 cut(s) 837, 958, 966
AluI AGCT 3 cut(s) 837, 958, 966
Alw21I GWGCWC 2 cut(s) 208, 960
Alw26I GTCTC 2 cut(s) 619, 981
AlwI GGATC 4 cut(s) 129, 426, 439, 1302
Ama87I CYCGRG 2 cut(s) 959, 1127
AoxI GGCC 8 cut(s) 39, 213, 234, 254, 322, 732, 1212, 1393
ApeKI GCWGC 3 cut(s) 5, 834, 1373
ApoI RAATTY 1 cut(s) 510
AseI ATTAAT 3 cut(s) 353, 701, 758
AspA2I CCTAGG 1 cut(s) 766
AspLEI GCGC 1 cut(s) 1238
AspS9I GGNCC 4 cut(s) 234, 1213, 1320, 1384
AsuC2I CCSGG 2 cut(s) 1128, 1129
AsuHPI GGTGA 1 cut(s) 1129
AvaI CYCGRG 2 cut(s) 959, 1127
AvaII GGWCC 2 cut(s) 1320, 1384
AvrII CCTAGG 1 cut(s) 766
BaeGI GKGCMC 2 cut(s) 563, 1273
BalI TGGCCA 1 cut(s) 215
BamHI GGATCC 1 cut(s) 431
BanII GRGCYC 3 cut(s) 960, 1175, 1483
Bbv12I GWGCWC 2 cut(s) 208, 960
BbvI GCAGC 3 cut(s) 17, 846, 1360
BccI CCATC 4 cut(s) 82, 269, 350, 1252
BceAI ACGGC 5 cut(s) 188, 221, 269, 785, 1361
BciT130I CCWGG 2 cut(s) 429, 557
BcnI CCSGG 2 cut(s) 1128, 1129
BcoDI GTCTC 2 cut(s) 619, 981
BfaI CTAG 5 cut(s) 258, 392, 521, 665, 767
BfoI RGCGCY 1 cut(s) 1239
BfrI CTTAAG 1 cut(s) 1033
BfuAI ACCTGC 1 cut(s) 669
BisI GCNGC 4 cut(s) 6, 778, 835, 1374
BlnI CCTAGG 1 cut(s) 766
BlsI GCNGC 4 cut(s) 7, 779, 836, 1375
Bme1390I CCNGG 4 cut(s) 429, 557, 1128, 1129
Bme18I GGWCC 2 cut(s) 1320, 1384
BmeT110I CYCGRG 2 cut(s) 959, 1127
BmgT120I GGNCC 4 cut(s) 234, 1213, 1320, 1384
BmiI GGNNCC 5 cut(s) 433, 882, 1174, 1385, 1480
BmrFI CCNGG 4 cut(s) 429, 557, 1128, 1129
BmsI GCATC 4 cut(s) 290, 584, 1459, 1513
BplI GAGNNNNNCTC 2 cut(s) 270, 302
BpmI CTGGAG 1 cut(s) 601
BpuEI CTTGAG 2 cut(s) 272, 887
BpuMI CCSGG 2 cut(s) 1128, 1129
Bsa29I ATCGAT 1 cut(s) 124
BsaHI GRCGYC 1 cut(s) 1418
BsaI GGTCTC 1 cut(s) 981
BsaXI ACNNNNNCTCC 2 cut(s) 1419, 1449
Bsc4I CCNNNNNNNGG 4 cut(s) 802, 938, 1157, 1344
Bse3DI GCAATG 1 cut(s) 277
BseBI CCWGG 2 cut(s) 429, 557
BseCI ATCGAT 1 cut(s) 124
BseGI GGATG 5 cut(s) 101, 281, 748, 842, 1244
BseLI CCNNNNNNNGG 4 cut(s) 802, 938, 1157, 1344
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 1291
BseRI GAGGAG 3 cut(s) 628, 808, 1500
BseSI GKGCMC 2 cut(s) 563, 1273
BseXI GCAGC 3 cut(s) 17, 846, 1360
Bsh1285I CGRYCG 2 cut(s) 321, 1345
BshFI GGCC 8 cut(s) 41, 215, 236, 256, 324, 734, 1214, 1395
BshVI ATCGAT 1 cut(s) 124
BsiEI CGRYCG 2 cut(s) 321, 1345
BsiHKAI GWGCWC 2 cut(s) 208, 960
BsiHKCI CYCGRG 2 cut(s) 959, 1127
BsiSI CCGG 1 cut(s) 1128
BslFI GGGAC 1 cut(s) 748
BslI CCNNNNNNNGG 4 cut(s) 802, 938, 1157, 1344
BsmAI GTCTC 2 cut(s) 619, 981
BsmBI CGTCTC 1 cut(s) 619
BsmFI GGGAC 1 cut(s) 748
BsnI GGCC 8 cut(s) 41, 215, 236, 256, 324, 734, 1214, 1395
Bso31I GGTCTC 1 cut(s) 981
BsoBI CYCGRG 2 cut(s) 959, 1127
Bsp1286I GDGCHC 6 cut(s) 208, 563, 960, 1175, 1273, 1483
Bsp143I GATC 6 cut(s) 121, 125, 318, 431, 1294, 1342
Bsp19I CCATGG 1 cut(s) 1412
BspACI CCGC 6 cut(s) 468, 777, 920, 999, 1308, 1442
BspANI GGCC 8 cut(s) 41, 215, 236, 256, 324, 734, 1214, 1395
BspCNI CTCAG 1 cut(s) 1290
BspDI ATCGAT 1 cut(s) 124
BspHI TCATGA 1 cut(s) 243
BspLI GGNNCC 5 cut(s) 433, 882, 1174, 1385, 1480
BspMI ACCTGC 1 cut(s) 669
BspPI GGATC 4 cut(s) 129, 426, 439, 1302
BspTI CTTAAG 1 cut(s) 1033
BspTNI GGTCTC 1 cut(s) 981
BsrDI GCAATG 1 cut(s) 277
BssMI GATC 6 cut(s) 121, 125, 318, 431, 1294, 1342
BssNI GRCGYC 1 cut(s) 1418
BssT1I CCWWGG 6 cut(s) 298, 766, 971, 1199, 1290, 1412
Bst2UI CCWGG 2 cut(s) 429, 557
Bst6I CTCTTC 2 cut(s) 826, 853
BstACI GRCGYC 1 cut(s) 1418
BstAFI CTTAAG 1 cut(s) 1033
BstAPI GCANNNNNTGC 1 cut(s) 1373
BstC8I GCNNGC 3 cut(s) 809, 813, 968
BstDEI CTNAG 2 cut(s) 1053, 1277
BstDSI CCRYGG 3 cut(s) 801, 1377, 1412
BstF5I GGATG 5 cut(s) 101, 281, 748, 842, 1244
BstH2I RGCGCY 1 cut(s) 1239
BstHHI GCGC 1 cut(s) 1238
BstKTI GATC 6 cut(s) 124, 128, 321, 434, 1297, 1345
BstMAI GTCTC 2 cut(s) 619, 981
BstMBI GATC 6 cut(s) 121, 125, 318, 431, 1294, 1342
BstMCI CGRYCG 2 cut(s) 321, 1345
BstMWI GCNNNNNNNGC 5 cut(s) 172, 212, 267, 1373, 1441
BstNI CCWGG 2 cut(s) 429, 557
BstNSI RCATGY 2 cut(s) 379, 1288
BstSCI CCNGG 4 cut(s) 427, 555, 1126, 1127
BstSLI GKGCMC 2 cut(s) 563, 1273
BstV1I GCAGC 3 cut(s) 17, 846, 1360
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
Bsu15I ATCGAT 1 cut(s) 124
BsuRI GGCC 8 cut(s) 41, 215, 236, 256, 324, 734, 1214, 1395
BsuTUI ATCGAT 1 cut(s) 124
BtgI CCRYGG 3 cut(s) 801, 1377, 1412
BtsCI GGATG 5 cut(s) 101, 281, 748, 842, 1244
BveI ACCTGC 1 cut(s) 669
Cac8I GCNNGC 3 cut(s) 809, 813, 968
CciI TCATGA 1 cut(s) 243
CfoI GCGC 1 cut(s) 1238
Cfr13I GGNCC 4 cut(s) 234, 1213, 1320, 1384
Cfr9I CCCGGG 1 cut(s) 1127
ClaI ATCGAT 1 cut(s) 124
CseI GACGC 2 cut(s) 1407, 1546
Csp6I GTAC 1 cut(s) 792
CviAII CATG 7 cut(s) 244, 376, 410, 828, 1285, 1364, 1413
CviQI GTAC 1 cut(s) 792
DdeI CTNAG 2 cut(s) 1053, 1277
DpnI GATC 6 cut(s) 123, 127, 320, 433, 1296, 1344
DpnII GATC 6 cut(s) 121, 125, 318, 431, 1294, 1342
DraI TTTAAA 2 cut(s) 387, 541
EaeI YGGCCR 4 cut(s) 39, 213, 322, 732
Eam1104I CTCTTC 2 cut(s) 826, 853
EarI CTCTTC 2 cut(s) 826, 853
Ecl136II GAGCTC 1 cut(s) 958
Eco130I CCWWGG 6 cut(s) 298, 766, 971, 1199, 1290, 1412
Eco24I GRGCYC 3 cut(s) 960, 1175, 1483
Eco31I GGTCTC 1 cut(s) 981
Eco47I GGWCC 2 cut(s) 1320, 1384
Eco53kI GAGCTC 1 cut(s) 958
Eco88I CYCGRG 2 cut(s) 959, 1127
EcoICRI GAGCTC 1 cut(s) 958
EcoO109I RGGNCCY 1 cut(s) 1384
EcoRII CCWGG 2 cut(s) 427, 555
EcoT14I CCWWGG 6 cut(s) 298, 766, 971, 1199, 1290, 1412
EcoT22I ATGCAT 1 cut(s) 381
EcoT38I GRGCYC 3 cut(s) 960, 1175, 1483
ErhI CCWWGG 6 cut(s) 298, 766, 971, 1199, 1290, 1412
Esp3I CGTCTC 1 cut(s) 619
FaeI CATG 7 cut(s) 247, 379, 413, 831, 1288, 1367, 1416
FalI AAGNNNNNCTT 2 cut(s) 920, 952
FaqI GGGAC 1 cut(s) 748
FatI CATG 7 cut(s) 243, 375, 409, 827, 1284, 1363, 1412
FauI CCCGC 2 cut(s) 1315, 1449
FblI GTMKAC 1 cut(s) 58
Fnu4HI GCNGC 4 cut(s) 6, 778, 835, 1374
FokI GGATG 5 cut(s) 88, 268, 755, 829, 1231
FriOI GRGCYC 3 cut(s) 960, 1175, 1483
Fsp4HI GCNGC 4 cut(s) 6, 778, 835, 1374
FspBI CTAG 5 cut(s) 258, 392, 521, 665, 767
GlaI GCGC 1 cut(s) 1237
GluI GCNGC 4 cut(s) 6, 778, 835, 1374
GsuI CTGGAG 1 cut(s) 601
HaeII RGCGCY 1 cut(s) 1239
HaeIII GGCC 8 cut(s) 41, 215, 236, 256, 324, 734, 1214, 1395
HapII CCGG 1 cut(s) 1128
HgaI GACGC 2 cut(s) 1407, 1546
HhaI GCGC 1 cut(s) 1238
Hin1I GRCGYC 1 cut(s) 1418
Hin1II CATG 7 cut(s) 247, 379, 413, 831, 1288, 1367, 1416
Hin6I GCGC 1 cut(s) 1236
HinP1I GCGC 1 cut(s) 1236
HinfI GANTC 2 cut(s) 186, 293
HpaII CCGG 1 cut(s) 1128
HphI GGTGA 1 cut(s) 1129
Hpy166II GTNNAC 4 cut(s) 59, 594, 1427, 1531
Hpy188I TCNGA 4 cut(s) 608, 981, 1280, 1522
Hpy188III TCNNGA 4 cut(s) 244, 346, 618, 961
Hpy8I GTNNAC 4 cut(s) 59, 594, 1427, 1531
HpyAV CCTTC 6 cut(s) 115, 652, 946, 1348, 1412, 1524
HpyCH4IV ACGT 3 cut(s) 612, 914, 1464
HpyF10VI GCNNNNNNNGC 5 cut(s) 172, 212, 267, 1373, 1441
HpyF3I CTNAG 2 cut(s) 1053, 1277
HpySE526I ACGT 3 cut(s) 612, 914, 1464
Hsp92I GRCGYC 1 cut(s) 1418
Hsp92II CATG 7 cut(s) 247, 379, 413, 831, 1288, 1367, 1416
HspAI GCGC 1 cut(s) 1236
Kzo9I GATC 6 cut(s) 121, 125, 318, 431, 1294, 1342
LmnI GCTCC 7 cut(s) 211, 278, 641, 963, 1178, 1440, 1478
Lsp1109I GCAGC 3 cut(s) 17, 846, 1360
LweI GCATC 4 cut(s) 290, 584, 1459, 1513
MaeI CTAG 5 cut(s) 258, 392, 521, 665, 767
MaeII ACGT 3 cut(s) 612, 914, 1464
MaeIII GTNAC 5 cut(s) 21, 449, 1028, 1059, 1504
MalI GATC 6 cut(s) 123, 127, 320, 433, 1296, 1344
MboI GATC 6 cut(s) 121, 125, 318, 431, 1294, 1342
MboII GAAGA 6 cut(s) 69, 813, 816, 840, 843, 1132
MfeI CAATTG 3 cut(s) 326, 402, 1225
MflI RGATCY 1 cut(s) 431
MhlI GDGCHC 6 cut(s) 208, 563, 960, 1175, 1273, 1483
MlsI TGGCCA 1 cut(s) 215
MluNI TGGCCA 1 cut(s) 215
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 908
Mox20I TGGCCA 1 cut(s) 215
Mph1103I ATGCAT 1 cut(s) 381
MscI TGGCCA 1 cut(s) 215
Msp20I TGGCCA 1 cut(s) 215
MspCI CTTAAG 1 cut(s) 1033
MspI CCGG 1 cut(s) 1128
MspR9I CCNGG 4 cut(s) 429, 557, 1128, 1129
MunI CAATTG 3 cut(s) 326, 402, 1225
MvaI CCWGG 2 cut(s) 429, 557
MwoI GCNNNNNNNGC 5 cut(s) 172, 212, 267, 1373, 1441
NciI CCSGG 2 cut(s) 1128, 1129
NcoI CCATGG 1 cut(s) 1412
NdeII GATC 6 cut(s) 121, 125, 318, 431, 1294, 1342
NlaIII CATG 7 cut(s) 247, 379, 413, 831, 1288, 1367, 1416
NlaIV GGNNCC 5 cut(s) 433, 882, 1174, 1385, 1480
NmeAIII GCCGAG 1 cut(s) 1470
NsiI ATGCAT 1 cut(s) 381
NspI RCATGY 2 cut(s) 379, 1288
PagI TCATGA 1 cut(s) 243
PfeI GAWTC 1 cut(s) 186
PkrI GCNGC 4 cut(s) 7, 779, 836, 1375
Ple19I CGATCG 2 cut(s) 321, 1345
PleI GAGTC 1 cut(s) 287
PpsI GAGTC 1 cut(s) 287
PpuMI RGGWCCY 1 cut(s) 1384
PshBI ATTAAT 3 cut(s) 353, 701, 758
PsiI TTATAA 1 cut(s) 336
Psp124BI GAGCTC 1 cut(s) 960
Psp5II RGGWCCY 1 cut(s) 1384
Psp6I CCWGG 2 cut(s) 427, 555
PspGI CCWGG 2 cut(s) 427, 555
PspN4I GGNNCC 5 cut(s) 433, 882, 1174, 1385, 1480
PspPI GGNCC 4 cut(s) 234, 1213, 1320, 1384
PspPPI RGGWCCY 1 cut(s) 1384
PsrI GAACNNNNNNTAC 2 cut(s) 705, 737
PsuI RGATCY 1 cut(s) 431
PvuI CGATCG 2 cut(s) 321, 1345
RsaI GTAC 1 cut(s) 793
RsaNI GTAC 1 cut(s) 792
SacI GAGCTC 1 cut(s) 960
SatI GCNGC 4 cut(s) 6, 778, 835, 1374
Sau3AI GATC 6 cut(s) 121, 125, 318, 431, 1294, 1342
Sau96I GGNCC 4 cut(s) 234, 1213, 1320, 1384
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 4 cut(s) 429, 557, 1128, 1129
SduI GDGCHC 6 cut(s) 208, 563, 960, 1175, 1273, 1483
SfaNI GCATC 4 cut(s) 290, 584, 1459, 1513
SinI GGWCC 2 cut(s) 1320, 1384
SmaI CCCGGG 1 cut(s) 1129
SmlI CTYRAG 3 cut(s) 287, 902, 1033
SmoI CTYRAG 3 cut(s) 287, 902, 1033
SsiI CCGC 6 cut(s) 468, 777, 920, 999, 1308, 1442
SspMI CTAG 5 cut(s) 258, 392, 521, 665, 767
SstI GAGCTC 1 cut(s) 960
StyD4I CCNGG 4 cut(s) 427, 555, 1126, 1127
StyI CCWWGG 6 cut(s) 298, 766, 971, 1199, 1290, 1412
TaiI ACGT 3 cut(s) 615, 917, 1467
TaqI TCGA 5 cut(s) 124, 1297, 1341, 1453, 1468
TaqII GACCGA 1 cut(s) 1308
TauI GCSGC 1 cut(s) 780
TfiI GAWTC 1 cut(s) 186
TseI GCWGC 3 cut(s) 5, 834, 1373
TspDTI ATGAA 5 cut(s) 232, 260, 816, 831, 1506
TspGWI ACGGA 1 cut(s) 264
TspMI CCCGGG 1 cut(s) 1127
Vha464I CTTAAG 1 cut(s) 1033
VpaK11BI GGWCC 2 cut(s) 1320, 1384
VspI ATTAAT 3 cut(s) 353, 701, 758
XapI RAATTY 1 cut(s) 510
XceI RCATGY 2 cut(s) 379, 1288
XcmI CCANNNNNNNNNTGG 1 cut(s) 724
XmaI CCCGGG 1 cut(s) 1127
XmaJI CCTAGG 1 cut(s) 766
XmiI GTMKAC 1 cut(s) 58
XspI CTAG 5 cut(s) 258, 392, 521, 665, 767
Zsp2I ATGCAT 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.