pycom01g03060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001044
Physical Location & Seq
Forward (+)
2452666 .. 2454563
1898 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03060.10

Sequence Viewer

Length: 1413 bp
ATGTCATATCTCATCTTACGAATTCGTTTCAATGTCATACATCCGTCAGTTTTTCTGTTAATTTTTCTATTAAATGCTAACGTGGACCCACTCCCTATAATCACCCTCACCCTTCTTCTTCCCCAGGTGGCCATGAACACACGCGGATTCAGCTCCAAGCTTTCAAGACTCTCTCATCCTAACTCATCATTGTATGCTTCCTCCACCTACCTCACTGATCAAAACCTCCAAACCCCTAAAAACCCTAACCACTTCACTCACTCTTCCAACTGCACTTTCTCCCCAAATCCTTTCAAATCTTCACCTAACCCCACCACCATTACTGAACACTACATAAAATCATCAAATTCAAAACCCATAAATCCTAATCCCACTTGGTCAAAAACCCAGATTTCGTCCTTGGAGGAAAGTGGGGCCGCCGTGGAGAAGTGGCATGTGTGGCGGGTGAGGGCTGCATGGCGAAGGCAATATAGAATAGACATGGCTTCTAGAGAGAGAAATGGGGGGGAGACGAATGAACTAGGGAAGAAGAAAGGTAAGCCGGAATTGGTTATCTGTCCGAGGCGCTTCTTCACAATGTGGTGTCGTTACAGGAGCTTCTCCATCTTGCTGGGGCATTGCATAAGGCTTTCGATGGCACTGCGTAAGGCTTTCGATGGCTCGCCATACTTGTATTTCCTTAGCTGCACTGTCTCCTTTTGGGTCCACGGCAGTGGCTGGGGCTTCTTAGCGGAGGCTGTGGGCGACGGTACATGGCAGAATGTAAATAACAAAGAGAAGAGGCTAGGGACGCAAATCTGGTTATGGAGTGTAAATGCCATCCTCTCCTTGGTGATTGCATGGGTTGCCTCCCACGAAGCGCTTGCTTTAACGTCTTGCAGCCGGACGATGGTGTGTTCATTCCCCATTGGAGCCCACGACATGCAATGGGATCTCCTCCACAACATGCCCTTCAACACTTTCATAATATGGCTCAATGAATGGGAGGGGGTAGTGATCCTCCCTGATGGTGGTGTTTTGGTTTCGAAAGTCCATGTAAATGCTCCCACCACCTTTAAATCGGTTGGGCACCTGCACCAAAGAAGTGAAGACTTACAAACATATTGTGGTGAAGGCTCAAAATCGGCAGCCCTATCAACAATGTCACCATGGCTACTTTCGGCCATATTGGGTGGTTGTAGGGCAATCACTCCAAGTTCTTCTCCAATGGTGGTTCTTGATACATCCTTCGTGATAAGTGTGGATCCTTTCGGCCCTATGTCCTTATTGAATTTAATTATATCACCACCAAACTCCATTGTTAGTGCTCCCTTGGCCACATCAATCTTTGTTTGGGCTGTTTTCATGAACGGACGGCCTAGCAAGATTGGCAATGATGGAGCATGGCTCGAATCCTCCATTTCTAAGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

471

Amino Acids

52.38

Weight (kDa)

9.39

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1080
Acc36I ACCTGC 1 cut(s) 1080
AccB1I GGYRCC 1 cut(s) 1068
AccII CGCG 1 cut(s) 144
AciI CCGC 4 cut(s) 144, 417, 442, 731
AclWI GGATC 4 cut(s) 939, 991, 1238, 1251
AcoI YGGCCR 3 cut(s) 129, 1161, 1314
AcsI RAATTY 3 cut(s) 21, 346, 1270
AdeI CACNNNGTG 1 cut(s) 579
AfaI GTAC 1 cut(s) 751
AfeI AGCGCT 1 cut(s) 861
AfiI CCNNNNNNNGG 3 cut(s) 829, 889, 1010
AgsI TTSAA 6 cut(s) 31, 165, 295, 351, 955, 1270
AjnI CCWGG 1 cut(s) 123
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
AleI CACNNNNGTG 1 cut(s) 711
AluBI AGCT 4 cut(s) 153, 160, 597, 684
AluI AGCT 4 cut(s) 153, 160, 597, 684
Alw21I GWGCWC 1 cut(s) 1309
Alw26I GTCTC 2 cut(s) 503, 697
AlwI GGATC 4 cut(s) 939, 991, 1238, 1251
AlwNI CAGNNNCTG 1 cut(s) 717
Aor51HI AGCGCT 1 cut(s) 861
AoxI GGCC 6 cut(s) 129, 414, 1161, 1252, 1314, 1355
ApeKI GCWGC 4 cut(s) 452, 684, 879, 1127
ApoI RAATTY 3 cut(s) 21, 346, 1270
AspLEI GCGC 2 cut(s) 567, 862
AspS9I GGNCC 4 cut(s) 85, 414, 703, 1253
AsuHPI GGTGA 8 cut(s) 94, 100, 294, 457, 844, 1121, 1137, 1275
AsuII TTCGAA 1 cut(s) 1025
AvaII GGWCC 2 cut(s) 85, 703
BaeGI GKGCMC 1 cut(s) 1071
BalI TGGCCA 2 cut(s) 131, 1316
BamHI GGATCC 1 cut(s) 1243
BanI GGYRCC 1 cut(s) 1068
BanII GRGCYC 1 cut(s) 916
BbsI GAAGAC 1 cut(s) 1095
Bbv12I GWGCWC 1 cut(s) 1309
BbvI GCAGC 4 cut(s) 439, 671, 891, 1139
BccI CCATC 7 cut(s) 611, 628, 650, 827, 883, 1001, 1370
BceAI ACGGC 3 cut(s) 404, 724, 1370
BcgI CGANNNNNNTGC 4 cut(s) 622, 656, 845, 879
BciT130I CCWGG 1 cut(s) 125
BclI TGATCA 1 cut(s) 217
BcoDI GTCTC 2 cut(s) 503, 697
BfaI CTAG 4 cut(s) 489, 521, 785, 1359
BfoI RGCGCY 2 cut(s) 568, 863
BfuAI ACCTGC 1 cut(s) 1080
BisI GCNGC 5 cut(s) 417, 453, 685, 880, 1128
BlsI GCNGC 5 cut(s) 418, 454, 686, 881, 1129
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 2 cut(s) 85, 703
BmgT120I GGNCC 4 cut(s) 85, 414, 703, 1253
BmiI GGNNCC 6 cut(s) 87, 415, 704, 913, 1070, 1245
BmrFI CCNGG 1 cut(s) 125
BpiI GAAGAC 1 cut(s) 1095
BplI GAGNNNNNCTC 2 cut(s) 1371, 1403
Bpu10I CCTNAGC 1 cut(s) 680
Bpu14I TTCGAA 1 cut(s) 1025
BsaJI CCNNGG 8 cut(s) 123, 399, 420, 560, 706, 828, 1148, 1311
Bsc4I CCNNNNNNNGG 3 cut(s) 829, 889, 1010
Bse3DI GCAATG 3 cut(s) 616, 932, 1378
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 8 cut(s) 123, 399, 420, 560, 706, 828, 1148, 1311
BseGI GGATG 4 cut(s) 40, 175, 819, 1223
BseLI CCNNNNNNNGG 3 cut(s) 829, 889, 1010
BseMI GCAATG 3 cut(s) 616, 932, 1378
BseRI GAGGAG 1 cut(s) 926
BseSI GKGCMC 1 cut(s) 1071
BseXI GCAGC 4 cut(s) 439, 671, 891, 1139
BseYI CCCAGC 2 cut(s) 610, 717
BsgI GTGCAG 3 cut(s) 256, 670, 1058
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 6 cut(s) 131, 416, 1163, 1254, 1316, 1357
BshNI GGYRCC 1 cut(s) 1068
BsiHKAI GWGCWC 1 cut(s) 1309
BsiSI CCGG 2 cut(s) 542, 883
BslFI GGGAC 1 cut(s) 802
BslI CCNNNNNNNGG 3 cut(s) 829, 889, 1010
BsmAI GTCTC 2 cut(s) 503, 697
BsmBI CGTCTC 1 cut(s) 503
BsmFI GGGAC 1 cut(s) 802
BsnI GGCC 6 cut(s) 131, 416, 1163, 1254, 1316, 1357
Bsp119I TTCGAA 1 cut(s) 1025
Bsp1286I GDGCHC 3 cut(s) 916, 1071, 1309
Bsp143I GATC 4 cut(s) 217, 931, 996, 1243
Bsp19I CCATGG 1 cut(s) 1148
BspACI CCGC 4 cut(s) 144, 417, 442, 731
BspANI GGCC 6 cut(s) 131, 416, 1163, 1254, 1316, 1357
BspFNI CGCG 1 cut(s) 144
BspHI TCATGA 1 cut(s) 1344
BspLI GGNNCC 6 cut(s) 87, 415, 704, 913, 1070, 1245
BspMI ACCTGC 1 cut(s) 1080
BspPI GGATC 4 cut(s) 939, 991, 1238, 1251
BspT104I TTCGAA 1 cut(s) 1025
BspT107I GGYRCC 1 cut(s) 1068
BsrDI GCAATG 3 cut(s) 616, 932, 1378
BssECI CCNNGG 8 cut(s) 123, 399, 420, 560, 706, 828, 1148, 1311
BssMI GATC 4 cut(s) 217, 931, 996, 1243
BssT1I CCWWGG 4 cut(s) 399, 828, 1148, 1311
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 691, 749
Bst6I CTCTTC 2 cut(s) 268, 773
BstAPI GCANNNNNTGC 1 cut(s) 845
BstBI TTCGAA 1 cut(s) 1025
BstC8I GCNNGC 2 cut(s) 662, 864
BstDEI CTNAG 3 cut(s) 680, 727, 1404
BstDSI CCRYGG 3 cut(s) 420, 706, 1148
BstF5I GGATG 4 cut(s) 40, 175, 819, 1223
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 2 cut(s) 568, 863
BstHHI GCGC 2 cut(s) 567, 862
BstKTI GATC 4 cut(s) 220, 934, 999, 1246
BstMAI GTCTC 2 cut(s) 503, 697
BstMBI GATC 4 cut(s) 217, 931, 996, 1243
BstMWI GCNNNNNNNGC 6 cut(s) 150, 439, 790, 845, 1313, 1368
BstNI CCWGG 1 cut(s) 125
BstNSI RCATGY 3 cut(s) 437, 925, 949
BstSCI CCNGG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 1071
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 4 cut(s) 439, 671, 891, 1139
BstV2I GAAGAC 1 cut(s) 1095
BstX2I RGATCY 2 cut(s) 931, 1243
BstXI CCANNNNNNTGG 2 cut(s) 610, 713
BstYI RGATCY 2 cut(s) 931, 1243
BsuRI GGCC 6 cut(s) 131, 416, 1163, 1254, 1316, 1357
BtgI CCRYGG 3 cut(s) 420, 706, 1148
BtsCI GGATG 4 cut(s) 40, 175, 819, 1223
BtsI GCAGTG 2 cut(s) 638, 718
BtsIMutI CAGTG 4 cut(s) 213, 638, 687, 718
BveI ACCTGC 1 cut(s) 1080
Cac8I GCNNGC 2 cut(s) 662, 864
CaiI CAGNNNCTG 1 cut(s) 717
CciI TCATGA 1 cut(s) 1344
CfoI GCGC 2 cut(s) 567, 862
Cfr13I GGNCC 4 cut(s) 85, 414, 703, 1253
CseI GACGC 1 cut(s) 799
Csp6I GTAC 1 cut(s) 750
CviQI GTAC 1 cut(s) 750
DdeI CTNAG 3 cut(s) 680, 727, 1404
DpnI GATC 4 cut(s) 219, 933, 998, 1245
DpnII GATC 4 cut(s) 217, 931, 996, 1243
DraI TTTAAA 1 cut(s) 1057
DraIII CACNNNGTG 1 cut(s) 579
EaeI YGGCCR 3 cut(s) 129, 1161, 1314
Eam1104I CTCTTC 2 cut(s) 268, 773
EarI CTCTTC 2 cut(s) 268, 773
Eco130I CCWWGG 4 cut(s) 399, 828, 1148, 1311
Eco24I GRGCYC 1 cut(s) 916
Eco47I GGWCC 2 cut(s) 85, 703
Eco47III AGCGCT 1 cut(s) 861
EcoRI GAATTC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 123
EcoT14I CCWWGG 4 cut(s) 399, 828, 1148, 1311
EcoT38I GRGCYC 1 cut(s) 916
ErhI CCWWGG 4 cut(s) 399, 828, 1148, 1311
Esp3I CGTCTC 1 cut(s) 503
FaqI GGGAC 1 cut(s) 802
FauI CCCGC 1 cut(s) 435
FbaI TGATCA 1 cut(s) 217
Fnu4HI GCNGC 5 cut(s) 417, 453, 685, 880, 1128
FokI GGATG 4 cut(s) 27, 162, 806, 1210
FriOI GRGCYC 1 cut(s) 916
Fsp4HI GCNGC 5 cut(s) 417, 453, 685, 880, 1128
FspBI CTAG 4 cut(s) 489, 521, 785, 1359
GlaI GCGC 2 cut(s) 566, 861
GluI GCNGC 5 cut(s) 417, 453, 685, 880, 1128
GsaI CCCAGC 2 cut(s) 614, 721
HaeII RGCGCY 2 cut(s) 568, 863
HaeIII GGCC 6 cut(s) 131, 416, 1163, 1254, 1316, 1357
HapII CCGG 2 cut(s) 542, 883
HgaI GACGC 1 cut(s) 799
HhaI GCGC 2 cut(s) 567, 862
Hin6I GCGC 2 cut(s) 565, 860
HinP1I GCGC 2 cut(s) 565, 860
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 3 cut(s) 147, 168, 1391
HpaII CCGG 2 cut(s) 542, 883
HphI GGTGA 8 cut(s) 94, 100, 294, 457, 844, 1121, 1137, 1275
Hpy166II GTNNAC 2 cut(s) 85, 706
Hpy188I TCNGA 1 cut(s) 561
Hpy188III TCNNGA 5 cut(s) 165, 489, 1217, 1231, 1345
Hpy8I GTNNAC 2 cut(s) 85, 706
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 5 cut(s) 122, 456, 961, 1106, 1237
HpyCH4III ACNGT 2 cut(s) 691, 749
HpyCH4IV ACGT 2 cut(s) 81, 872
HpyCH4V TGCA 8 cut(s) 273, 455, 621, 687, 839, 879, 925, 1075
HpyF10VI GCNNNNNNNGC 6 cut(s) 150, 439, 790, 845, 1313, 1368
HpyF3I CTNAG 3 cut(s) 680, 727, 1404
HpySE526I ACGT 2 cut(s) 81, 872
HspAI GCGC 2 cut(s) 565, 860
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 4 cut(s) 217, 931, 996, 1243
LmnI GCTCC 6 cut(s) 158, 594, 911, 1048, 1312, 1379
Lsp1109I GCAGC 4 cut(s) 439, 671, 891, 1139
MaeI CTAG 4 cut(s) 489, 521, 785, 1359
MaeII ACGT 2 cut(s) 81, 872
MaeIII GTNAC 2 cut(s) 587, 1143
MalI GATC 4 cut(s) 219, 933, 998, 1245
MboI GATC 4 cut(s) 217, 931, 996, 1243
MflI RGATCY 2 cut(s) 931, 1243
MhlI GDGCHC 3 cut(s) 916, 1071, 1309
MlsI TGGCCA 2 cut(s) 131, 1316
MluCI AATT 6 cut(s) 21, 60, 346, 545, 1270, 1275
MluNI TGGCCA 2 cut(s) 131, 1316
MlyI GAGTC 1 cut(s) 162
MmeI TCCRAC 1 cut(s) 291
Mox20I TGGCCA 2 cut(s) 131, 1316
MscI TGGCCA 2 cut(s) 131, 1316
MseI TTAA 5 cut(s) 59, 71, 869, 1056, 1274
MslI CAYNNNNRTG 2 cut(s) 711, 1038
Msp20I TGGCCA 2 cut(s) 131, 1316
MspI CCGG 2 cut(s) 542, 883
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 6 cut(s) 150, 439, 790, 845, 1313, 1368
NcoI CCATGG 1 cut(s) 1148
NdeII GATC 4 cut(s) 217, 931, 996, 1243
NlaIV GGNNCC 6 cut(s) 87, 415, 704, 913, 1070, 1245
NmuCI GTSAC 1 cut(s) 1143
NspI RCATGY 3 cut(s) 437, 925, 949
NspV TTCGAA 1 cut(s) 1025
OliI CACNNNNGTG 1 cut(s) 711
PagI TCATGA 1 cut(s) 1344
PaqCI CACCTGC 1 cut(s) 1080
PfeI GAWTC 2 cut(s) 147, 1391
PkrI GCNGC 5 cut(s) 418, 454, 686, 881, 1129
PleI GAGTC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 123
PspFI CCCAGC 2 cut(s) 610, 717
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 6 cut(s) 87, 415, 704, 913, 1070, 1245
PspPI GGNCC 4 cut(s) 85, 414, 703, 1253
PstNI CAGNNNCTG 1 cut(s) 717
PsuI RGATCY 2 cut(s) 931, 1243
RsaI GTAC 1 cut(s) 751
RsaNI GTAC 1 cut(s) 750
RseI CAYNNNNRTG 2 cut(s) 711, 1038
SaqAI TTAA 5 cut(s) 59, 71, 869, 1056, 1274
SatI GCNGC 5 cut(s) 417, 453, 685, 880, 1128
Sau3AI GATC 4 cut(s) 217, 931, 996, 1243
Sau96I GGNCC 4 cut(s) 85, 414, 703, 1253
SchI GAGTC 1 cut(s) 162
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 3 cut(s) 916, 1071, 1309
SfuI TTCGAA 1 cut(s) 1025
SinI GGWCC 2 cut(s) 85, 703
SmiMI CAYNNNNRTG 2 cut(s) 711, 1038
Sse9I AATT 6 cut(s) 21, 60, 346, 545, 1270, 1275
SsiI CCGC 4 cut(s) 144, 417, 442, 731
SspMI CTAG 4 cut(s) 489, 521, 785, 1359
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 4 cut(s) 399, 828, 1148, 1311
TaaI ACNGT 2 cut(s) 691, 749
TaiI ACGT 2 cut(s) 84, 875
TaqI TCGA 4 cut(s) 632, 654, 1025, 1389
TasI AATT 6 cut(s) 21, 60, 346, 545, 1270, 1275
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 2 cut(s) 147, 1391
Tru1I TTAA 5 cut(s) 59, 71, 869, 1056, 1274
Tru9I TTAA 5 cut(s) 59, 71, 869, 1056, 1274
TscAI CASTG 4 cut(s) 220, 645, 694, 718
TseFI GTSAC 1 cut(s) 1143
TseI GCWGC 4 cut(s) 452, 684, 879, 1127
Tsp45I GTSAC 1 cut(s) 1143
TspDTI ATGAA 7 cut(s) 149, 531, 888, 952, 993, 1333, 1361
TspGWI ACGGA 2 cut(s) 33, 1365
TspRI CASTG 4 cut(s) 220, 645, 694, 718
VpaK11BI GGWCC 2 cut(s) 85, 703
XapI RAATTY 3 cut(s) 21, 346, 1270
XbaI TCTAGA 1 cut(s) 488
XceI RCATGY 3 cut(s) 437, 925, 949
XcmI CCANNNNNNNNNTGG 1 cut(s) 826
XspI CTAG 4 cut(s) 489, 521, 785, 1359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.