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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 57 |
| AccI | GTMKAC | 1 cut(s) | 249 |
| AcsI | RAATTY | 1 cut(s) | 226 |
| AgsI | TTSAA | 1 cut(s) | 26 |
| AluBI | AGCT | 1 cut(s) | 78 |
| AluI | AGCT | 1 cut(s) | 78 |
| Ama87I | CYCGRG | 1 cut(s) | 6 |
| AoxI | GGCC | 1 cut(s) | 290 |
| ApeKI | GCWGC | 1 cut(s) | 13 |
| ApoI | RAATTY | 1 cut(s) | 226 |
| AspS9I | GGNCC | 1 cut(s) | 168 |
| AsuHPI | GGTGA | 1 cut(s) | 86 |
| AvaI | CYCGRG | 1 cut(s) | 6 |
| AvaII | GGWCC | 1 cut(s) | 168 |
| BaeGI | GKGCMC | 1 cut(s) | 49 |
| BbvI | GCAGC | 1 cut(s) | 25 |
| BccI | CCATC | 2 cut(s) | 229, 305 |
| BceAI | ACGGC | 2 cut(s) | 57, 305 |
| BfaI | CTAG | 1 cut(s) | 294 |
| BisI | GCNGC | 1 cut(s) | 14 |
| BlsI | GCNGC | 1 cut(s) | 15 |
| Bme18I | GGWCC | 1 cut(s) | 168 |
| BmeT110I | CYCGRG | 1 cut(s) | 6 |
| BmgT120I | GGNCC | 1 cut(s) | 168 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 306, 338 |
| BsaJI | CCNNGG | 1 cut(s) | 231 |
| Bse3DI | GCAATG | 1 cut(s) | 313 |
| BseDI | CCNNGG | 1 cut(s) | 231 |
| BseMI | GCAATG | 1 cut(s) | 313 |
| BseSI | GKGCMC | 1 cut(s) | 49 |
| BseXI | GCAGC | 1 cut(s) | 25 |
| BshFI | GGCC | 1 cut(s) | 292 |
| BsiHKCI | CYCGRG | 1 cut(s) | 6 |
| BslFI | GGGAC | 1 cut(s) | 202 |
| BsmFI | GGGAC | 1 cut(s) | 202 |
| BsnI | GGCC | 1 cut(s) | 292 |
| BsoBI | CYCGRG | 1 cut(s) | 6 |
| Bsp1286I | GDGCHC | 1 cut(s) | 49 |
| Bsp19I | CCATGG | 1 cut(s) | 231 |
| BspANI | GGCC | 1 cut(s) | 292 |
| BspHI | TCATGA | 1 cut(s) | 279 |
| BsrDI | GCAATG | 1 cut(s) | 313 |
| BssECI | CCNNGG | 1 cut(s) | 231 |
| BssNAI | GTATAC | 1 cut(s) | 250 |
| BssT1I | CCWWGG | 1 cut(s) | 231 |
| Bst1107I | GTATAC | 1 cut(s) | 250 |
| BstC8I | GCNNGC | 1 cut(s) | 80 |
| BstDEI | CTNAG | 1 cut(s) | 339 |
| BstDSI | CCRYGG | 1 cut(s) | 231 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 303 |
| BstSLI | GKGCMC | 1 cut(s) | 49 |
| BstV1I | GCAGC | 1 cut(s) | 25 |
| BstZ17I | GTATAC | 1 cut(s) | 250 |
| BsuRI | GGCC | 1 cut(s) | 292 |
| BtgI | CCRYGG | 1 cut(s) | 231 |
| Cac8I | GCNNGC | 1 cut(s) | 80 |
| CciI | TCATGA | 1 cut(s) | 279 |
| Cfr13I | GGNCC | 1 cut(s) | 168 |
| CviAII | CATG | 4 cut(s) | 69, 232, 280, 318 |
| CviJI | RGCY | 3 cut(s) | 78, 292, 322 |
| CviKI_1 | RGCY | 3 cut(s) | 78, 292, 322 |
| DdeI | CTNAG | 1 cut(s) | 339 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 57 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 57 |
| Eco130I | CCWWGG | 1 cut(s) | 231 |
| Eco47I | GGWCC | 1 cut(s) | 168 |
| Eco88I | CYCGRG | 1 cut(s) | 6 |
| EcoT14I | CCWWGG | 1 cut(s) | 231 |
| ErhI | CCWWGG | 1 cut(s) | 231 |
| FaeI | CATG | 4 cut(s) | 72, 235, 283, 321 |
| FaiI | YATR | 7 cut(s) | 57, 70, 233, 250, 281, 319, 346 |
| FaqI | GGGAC | 1 cut(s) | 202 |
| FatI | CATG | 4 cut(s) | 68, 231, 279, 317 |
| FblI | GTMKAC | 1 cut(s) | 249 |
| Fnu4HI | GCNGC | 1 cut(s) | 14 |
| Fsp4HI | GCNGC | 1 cut(s) | 14 |
| FspBI | CTAG | 1 cut(s) | 294 |
| GluI | GCNGC | 1 cut(s) | 14 |
| HaeIII | GGCC | 1 cut(s) | 292 |
| Hin1II | CATG | 4 cut(s) | 72, 235, 283, 321 |
| HinfI | GANTC | 5 cut(s) | 4, 64, 87, 137, 326 |
| HphI | GGTGA | 1 cut(s) | 86 |
| Hpy166II | GTNNAC | 1 cut(s) | 250 |
| Hpy188I | TCNGA | 2 cut(s) | 63, 196 |
| Hpy188III | TCNNGA | 1 cut(s) | 280 |
| Hpy8I | GTNNAC | 1 cut(s) | 250 |
| HpyCH4V | TGCA | 2 cut(s) | 82, 123 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 303 |
| HpyF3I | CTNAG | 1 cut(s) | 339 |
| Hsp92II | CATG | 4 cut(s) | 72, 235, 283, 321 |
| LmnI | GCTCC | 2 cut(s) | 114, 314 |
| LpnPI | CCDG | 1 cut(s) | 139 |
| Lsp1109I | GCAGC | 1 cut(s) | 25 |
| MaeI | CTAG | 1 cut(s) | 294 |
| MfeI | CAATTG | 1 cut(s) | 174 |
| MhlI | GDGCHC | 1 cut(s) | 49 |
| MluCI | AATT | 3 cut(s) | 101, 174, 226 |
| MnlI | CCTC | 4 cut(s) | 102, 108, 181, 340 |
| MseI | TTAA | 3 cut(s) | 185, 203, 237 |
| MunI | CAATTG | 1 cut(s) | 174 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 303 |
| NcoI | CCATGG | 1 cut(s) | 231 |
| NlaIII | CATG | 4 cut(s) | 72, 235, 283, 321 |
| PaeR7I | CTCGAG | 1 cut(s) | 6 |
| PagI | TCATGA | 1 cut(s) | 279 |
| PfeI | GAWTC | 4 cut(s) | 64, 87, 137, 326 |
| PkrI | GCNGC | 1 cut(s) | 15 |
| PspPI | GGNCC | 1 cut(s) | 168 |
| PspXI | VCTCGAGB | 1 cut(s) | 6 |
| SaqAI | TTAA | 3 cut(s) | 185, 203, 237 |
| SatI | GCNGC | 1 cut(s) | 14 |
| Sau96I | GGNCC | 1 cut(s) | 168 |
| SduI | GDGCHC | 1 cut(s) | 49 |
| SetI | ASST | 2 cut(s) | 80, 128 |
| Sfr274I | CTCGAG | 1 cut(s) | 6 |
| SinI | GGWCC | 1 cut(s) | 168 |
| SlaI | CTCGAG | 1 cut(s) | 6 |
| SmlI | CTYRAG | 1 cut(s) | 6 |
| SmoI | CTYRAG | 1 cut(s) | 6 |
| Sse9I | AATT | 3 cut(s) | 101, 174, 226 |
| SspMI | CTAG | 1 cut(s) | 294 |
| StyI | CCWWGG | 1 cut(s) | 231 |
| TaqI | TCGA | 2 cut(s) | 7, 324 |
| TasI | AATT | 3 cut(s) | 101, 174, 226 |
| TfiI | GAWTC | 4 cut(s) | 64, 87, 137, 326 |
| Tru1I | TTAA | 3 cut(s) | 185, 203, 237 |
| Tru9I | TTAA | 3 cut(s) | 185, 203, 237 |
| TseI | GCWGC | 1 cut(s) | 13 |
| TspDTI | ATGAA | 2 cut(s) | 268, 296 |
| VpaK11BI | GGWCC | 1 cut(s) | 168 |
| XapI | RAATTY | 1 cut(s) | 226 |
| XhoI | CTCGAG | 1 cut(s) | 6 |
| XmiI | GTMKAC | 1 cut(s) | 249 |
| XspI | CTAG | 1 cut(s) | 294 |