pycom17g18490

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001044
Physical Location & Seq
Forward (+)
15878552 .. 15879030
479 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g18490.2

Sequence Viewer

Length: 438 bp
ATGAAGATGGTGGAGAGGGTTCTGATCCATCGACATGTCGAGCAGTCGATTGGCCAAAAAATGAATGATTTACTAGACGCAATACAAAGCAACAGTGATACACATACCAGGATGCTTGAACTATTAGTCAGTAAAGCCTTCGAAGATGGCCATATTGGCCAACCTCGGCAGCTTTCTTTTACAAATGTTCTAGTACAAGCTGAAAAAAGGTTCGCTCGGTTACAACCAATTGACTTAGAGAAAAGAGGCGGATCAGGCAATAGATCTGACGGACCGAACCAGGGAGAAGAAGCAGCATCCGTGATGCGATATTTATACGCACACAAACTAAACCCTATTTGTGACAATTGTAGTAATGATGTAAGTAGGGATCGTTCTAACCGGGGATTAACTAGGGATGCTAAACACTTGACTCAAATAAATAAAACTAACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

146

Amino Acids

16.47

Weight (kDa)

9.03

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 249
AclWI GGATC 3 cut(s) 19, 259, 378
AcoI YGGCCR 3 cut(s) 52, 148, 157
AfaI GTAC 1 cut(s) 195
AfiI CCNNNNNNNGG 1 cut(s) 281
AflIII ACRYGT 1 cut(s) 34
AgsI TTSAA 1 cut(s) 119
AjnI CCWGG 2 cut(s) 107, 279
AluBI AGCT 2 cut(s) 172, 200
AluI AGCT 2 cut(s) 172, 200
AlwI GGATC 3 cut(s) 19, 259, 378
AoxI GGCC 3 cut(s) 52, 148, 157
ApeKI GCWGC 2 cut(s) 169, 293
AspS9I GGNCC 1 cut(s) 272
AsuC2I CCSGG 1 cut(s) 383
AsuII TTCGAA 1 cut(s) 141
AvaII GGWCC 1 cut(s) 272
BalI TGGCCA 3 cut(s) 54, 150, 159
BbvI GCAGC 2 cut(s) 181, 305
BccI CCATC 2 cut(s) 36, 140
BciT130I CCWGG 2 cut(s) 109, 281
BcnI CCSGG 1 cut(s) 383
BfaI CTAG 3 cut(s) 74, 191, 393
BglI GCCNNNNNGGC 1 cut(s) 156
BglII AGATCT 1 cut(s) 263
BisI GCNGC 2 cut(s) 170, 294
BlsI GCNGC 2 cut(s) 171, 295
Bme1390I CCNGG 3 cut(s) 109, 281, 383
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 272
BmrFI CCNGG 3 cut(s) 109, 281, 383
BmsI GCATC 4 cut(s) 102, 294, 305, 388
Bpu14I TTCGAA 1 cut(s) 141
BpuMI CCSGG 1 cut(s) 383
BsaJI CCNNGG 3 cut(s) 164, 280, 382
Bsc4I CCNNNNNNNGG 1 cut(s) 281
BseBI CCWGG 2 cut(s) 109, 281
BseDI CCNNGG 3 cut(s) 164, 280, 382
BseGI GGATG 3 cut(s) 117, 296, 403
BseLI CCNNNNNNNGG 1 cut(s) 281
BseXI GCAGC 2 cut(s) 181, 305
BshFI GGCC 3 cut(s) 54, 150, 159
BsiSI CCGG 1 cut(s) 382
BslI CCNNNNNNNGG 1 cut(s) 281
BsnI GGCC 3 cut(s) 54, 150, 159
Bsp119I TTCGAA 1 cut(s) 141
Bsp143I GATC 4 cut(s) 24, 251, 263, 370
BspACI CCGC 1 cut(s) 249
BspANI GGCC 3 cut(s) 54, 150, 159
BspPI GGATC 3 cut(s) 19, 259, 378
BspT104I TTCGAA 1 cut(s) 141
BssECI CCNNGG 3 cut(s) 164, 280, 382
BssMI GATC 4 cut(s) 24, 251, 263, 370
Bst2UI CCWGG 2 cut(s) 109, 281
Bst4CI ACNGT 1 cut(s) 95
BstBI TTCGAA 1 cut(s) 141
BstDEI CTNAG 1 cut(s) 235
BstF5I GGATG 3 cut(s) 117, 296, 403
BstKTI GATC 4 cut(s) 27, 254, 266, 373
BstMBI GATC 4 cut(s) 24, 251, 263, 370
BstMWI GCNNNNNNNGC 2 cut(s) 156, 255
BstNI CCWGG 2 cut(s) 109, 281
BstNSI RCATGY 1 cut(s) 38
BstSCI CCNGG 3 cut(s) 107, 279, 381
BstV1I GCAGC 2 cut(s) 181, 305
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BsuRI GGCC 3 cut(s) 54, 150, 159
BtsCI GGATG 3 cut(s) 117, 296, 403
BtsIMutI CAGTG 1 cut(s) 100
Cfr13I GGNCC 1 cut(s) 272
CpoI CGGWCCG 1 cut(s) 272
CseI GACGC 1 cut(s) 86
Csp6I GTAC 1 cut(s) 194
CspI CGGWCCG 1 cut(s) 272
CviAII CATG 1 cut(s) 35
CviJI RGCY 6 cut(s) 54, 137, 150, 159, 172, 200
CviKI_1 RGCY 6 cut(s) 54, 137, 150, 159, 172, 200
CviQI GTAC 1 cut(s) 194
DdeI CTNAG 1 cut(s) 235
DpnI GATC 4 cut(s) 26, 253, 265, 372
DpnII GATC 4 cut(s) 24, 251, 263, 370
EaeI YGGCCR 3 cut(s) 52, 148, 157
EciI GGCGGA 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 107, 279
FaeI CATG 1 cut(s) 38
FaiI YATR 4 cut(s) 36, 105, 153, 316
FatI CATG 1 cut(s) 34
Fnu4HI GCNGC 2 cut(s) 170, 294
FokI GGATG 3 cut(s) 124, 283, 410
Fsp4HI GCNGC 2 cut(s) 170, 294
FspBI CTAG 3 cut(s) 74, 191, 393
GluI GCNGC 2 cut(s) 170, 294
HaeIII GGCC 3 cut(s) 54, 150, 159
HapII CCGG 1 cut(s) 382
HgaI GACGC 1 cut(s) 86
Hin1II CATG 1 cut(s) 38
HinfI GANTC 1 cut(s) 412
HpaII CCGG 1 cut(s) 382
Hpy188I TCNGA 2 cut(s) 24, 268
HpyAV CCTTC 1 cut(s) 148
HpyCH4III ACNGT 1 cut(s) 95
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 255
HpyF3I CTNAG 1 cut(s) 235
Hsp92II CATG 1 cut(s) 38
Kzo9I GATC 4 cut(s) 24, 251, 263, 370
LpnPI CCDG 6 cut(s) 94, 121, 240, 266, 293, 395
Lsp1109I GCAGC 2 cut(s) 181, 305
LweI GCATC 4 cut(s) 102, 294, 305, 388
MaeI CTAG 3 cut(s) 74, 191, 393
MaeIII GTNAC 2 cut(s) 219, 341
MalI GATC 4 cut(s) 26, 253, 265, 372
MboI GATC 4 cut(s) 24, 251, 263, 370
MboII GAAGA 3 cut(s) 16, 155, 299
MfeI CAATTG 2 cut(s) 228, 346
MflI RGATCY 1 cut(s) 263
MlsI TGGCCA 3 cut(s) 54, 150, 159
MluCI AATT 2 cut(s) 228, 346
MluNI TGGCCA 3 cut(s) 54, 150, 159
MlyI GAGTC 1 cut(s) 406
MnlI CCTC 3 cut(s) 9, 174, 239
Mox20I TGGCCA 3 cut(s) 54, 150, 159
MscI TGGCCA 3 cut(s) 54, 150, 159
MseI TTAA 2 cut(s) 389, 436
MslI CAYNNNNRTG 1 cut(s) 33
Msp20I TGGCCA 3 cut(s) 54, 150, 159
MspI CCGG 1 cut(s) 382
MspR9I CCNGG 3 cut(s) 109, 281, 383
MunI CAATTG 2 cut(s) 228, 346
MvaI CCWGG 2 cut(s) 109, 281
MwoI GCNNNNNNNGC 2 cut(s) 156, 255
NciI CCSGG 1 cut(s) 383
NdeII GATC 4 cut(s) 24, 251, 263, 370
NlaIII CATG 1 cut(s) 38
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 1 cut(s) 341
NspI RCATGY 1 cut(s) 38
NspV TTCGAA 1 cut(s) 141
PciI ACATGT 1 cut(s) 34
PkrI GCNGC 2 cut(s) 171, 295
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
PscI ACATGT 1 cut(s) 34
Psp6I CCWGG 2 cut(s) 107, 279
PspGI CCWGG 2 cut(s) 107, 279
PspPI GGNCC 1 cut(s) 272
PsrI GAACNNNNNNTAC 2 cut(s) 358, 390
PsuI RGATCY 1 cut(s) 263
RsaI GTAC 1 cut(s) 195
RsaNI GTAC 1 cut(s) 194
RseI CAYNNNNRTG 1 cut(s) 33
Rsr2I CGGWCCG 1 cut(s) 272
RsrII CGGWCCG 1 cut(s) 272
SaqAI TTAA 2 cut(s) 389, 436
SatI GCNGC 2 cut(s) 170, 294
Sau3AI GATC 4 cut(s) 24, 251, 263, 370
Sau96I GGNCC 1 cut(s) 272
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 3 cut(s) 109, 281, 383
SetI ASST 4 cut(s) 166, 174, 202, 212
SfaNI GCATC 4 cut(s) 102, 294, 305, 388
SfiI GGCCNNNNNGGCC 1 cut(s) 156
SfuI TTCGAA 1 cut(s) 141
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 33
Sse9I AATT 2 cut(s) 228, 346
SsiI CCGC 1 cut(s) 249
SspMI CTAG 3 cut(s) 74, 191, 393
StyD4I CCNGG 3 cut(s) 107, 279, 381
TaaI ACNGT 1 cut(s) 95
TaqI TCGA 4 cut(s) 31, 39, 47, 141
TaqII GACCGA 1 cut(s) 289
TasI AATT 2 cut(s) 228, 346
TatI WGTACW 1 cut(s) 193
Tru1I TTAA 2 cut(s) 389, 436
Tru9I TTAA 2 cut(s) 389, 436
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 2 cut(s) 169, 293
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 2 cut(s) 17, 77
TspGWI ACGGA 2 cut(s) 285, 289
TspRI CASTG 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 272
XceI RCATGY 1 cut(s) 38
XspI CTAG 3 cut(s) 74, 191, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.