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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 131 |
| AccB1I | GGYRCC | 1 cut(s) | 238 |
| AccI | GTMKAC | 2 cut(s) | 119, 248 |
| AcsI | RAATTY | 2 cut(s) | 10, 223 |
| AcyI | GRCGYC | 1 cut(s) | 95 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 27, 38, 336 |
| AgsI | TTSAA | 2 cut(s) | 106, 114 |
| AjnI | CCWGG | 1 cut(s) | 89 |
| AoxI | GGCC | 5 cut(s) | 29, 40, 69, 267, 283 |
| ApoI | RAATTY | 2 cut(s) | 10, 223 |
| AspLEI | GCGC | 1 cut(s) | 128 |
| AspS9I | GGNCC | 2 cut(s) | 41, 267 |
| BanI | GGYRCC | 1 cut(s) | 238 |
| BbsI | GAAGAC | 1 cut(s) | 265 |
| BccI | CCATC | 2 cut(s) | 73, 226 |
| BceAI | ACGGC | 2 cut(s) | 10, 254 |
| BciT130I | CCWGG | 1 cut(s) | 91 |
| BfaI | CTAG | 1 cut(s) | 336 |
| BfuAI | ACCTGC | 1 cut(s) | 131 |
| Bme1390I | CCNGG | 1 cut(s) | 91 |
| BmgT120I | GGNCC | 2 cut(s) | 41, 267 |
| BmiI | GGNNCC | 1 cut(s) | 240 |
| BmrFI | CCNGG | 1 cut(s) | 91 |
| BpiI | GAAGAC | 1 cut(s) | 265 |
| Bsa29I | ATCGAT | 1 cut(s) | 137 |
| BsaHI | GRCGYC | 1 cut(s) | 95 |
| BsaJI | CCNNGG | 3 cut(s) | 26, 90, 290 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 27, 38, 336 |
| BseBI | CCWGG | 1 cut(s) | 91 |
| BseCI | ATCGAT | 1 cut(s) | 137 |
| BseDI | CCNNGG | 3 cut(s) | 26, 90, 290 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 27, 38, 336 |
| BseRI | GAGGAG | 1 cut(s) | 7 |
| BshFI | GGCC | 5 cut(s) | 31, 42, 71, 269, 285 |
| BshNI | GGYRCC | 1 cut(s) | 238 |
| BshVI | ATCGAT | 1 cut(s) | 137 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 27, 38, 336 |
| BsnI | GGCC | 5 cut(s) | 31, 42, 71, 269, 285 |
| BspANI | GGCC | 5 cut(s) | 31, 42, 71, 269, 285 |
| BspDI | ATCGAT | 1 cut(s) | 137 |
| BspHI | TCATGA | 1 cut(s) | 276 |
| BspLI | GGNNCC | 1 cut(s) | 240 |
| BspMI | ACCTGC | 1 cut(s) | 131 |
| BspT107I | GGYRCC | 1 cut(s) | 238 |
| BssECI | CCNNGG | 3 cut(s) | 26, 90, 290 |
| BssNAI | GTATAC | 2 cut(s) | 120, 249 |
| BssNI | GRCGYC | 1 cut(s) | 95 |
| BssT1I | CCWWGG | 1 cut(s) | 290 |
| Bst1107I | GTATAC | 2 cut(s) | 120, 249 |
| Bst2UI | CCWGG | 1 cut(s) | 91 |
| BstACI | GRCGYC | 1 cut(s) | 95 |
| BstDEI | CTNAG | 1 cut(s) | 73 |
| BstDSI | CCRYGG | 1 cut(s) | 26 |
| BstHHI | GCGC | 1 cut(s) | 128 |
| BstNI | CCWGG | 1 cut(s) | 91 |
| BstSCI | CCNGG | 1 cut(s) | 89 |
| BstV2I | GAAGAC | 1 cut(s) | 265 |
| BstZ17I | GTATAC | 2 cut(s) | 120, 249 |
| Bsu15I | ATCGAT | 1 cut(s) | 137 |
| BsuRI | GGCC | 5 cut(s) | 31, 42, 71, 269, 285 |
| BsuTUI | ATCGAT | 1 cut(s) | 137 |
| BtgI | CCRYGG | 1 cut(s) | 26 |
| BveI | ACCTGC | 1 cut(s) | 131 |
| CciI | TCATGA | 1 cut(s) | 276 |
| CfoI | GCGC | 1 cut(s) | 128 |
| Cfr13I | GGNCC | 2 cut(s) | 41, 267 |
| ClaI | ATCGAT | 1 cut(s) | 137 |
| CseI | GACGC | 1 cut(s) | 84 |
| CviAII | CATG | 2 cut(s) | 277, 331 |
| CviJI | RGCY | 6 cut(s) | 31, 42, 71, 269, 285, 320 |
| CviKI_1 | RGCY | 6 cut(s) | 31, 42, 71, 269, 285, 320 |
| DdeI | CTNAG | 1 cut(s) | 73 |
| Eco130I | CCWWGG | 1 cut(s) | 290 |
| Eco32I | GATATC | 1 cut(s) | 179 |
| EcoRI | GAATTC | 2 cut(s) | 10, 223 |
| EcoRII | CCWGG | 1 cut(s) | 89 |
| EcoRV | GATATC | 1 cut(s) | 179 |
| EcoT14I | CCWWGG | 1 cut(s) | 290 |
| ErhI | CCWWGG | 1 cut(s) | 290 |
| FaeI | CATG | 2 cut(s) | 280, 334 |
| FaiI | YATR | 6 cut(s) | 68, 120, 185, 249, 278, 332 |
| FatI | CATG | 2 cut(s) | 276, 330 |
| FblI | GTMKAC | 2 cut(s) | 119, 248 |
| FspBI | CTAG | 1 cut(s) | 336 |
| GlaI | GCGC | 1 cut(s) | 127 |
| HaeIII | GGCC | 5 cut(s) | 31, 42, 71, 269, 285 |
| HgaI | GACGC | 1 cut(s) | 84 |
| HhaI | GCGC | 1 cut(s) | 128 |
| Hin1I | GRCGYC | 1 cut(s) | 95 |
| Hin1II | CATG | 2 cut(s) | 280, 334 |
| Hin6I | GCGC | 1 cut(s) | 126 |
| HinP1I | GCGC | 1 cut(s) | 126 |
| HinfI | GANTC | 3 cut(s) | 134, 297, 323 |
| Hpy166II | GTNNAC | 2 cut(s) | 120, 249 |
| Hpy188I | TCNGA | 1 cut(s) | 193 |
| Hpy188III | TCNNGA | 1 cut(s) | 277 |
| Hpy8I | GTNNAC | 2 cut(s) | 120, 249 |
| HpyAV | CCTTC | 1 cut(s) | 60 |
| HpyCH4IV | ACGT | 1 cut(s) | 341 |
| HpyCH4V | TGCA | 2 cut(s) | 36, 61 |
| HpyF3I | CTNAG | 1 cut(s) | 73 |
| HpySE526I | ACGT | 1 cut(s) | 341 |
| Hsp92I | GRCGYC | 1 cut(s) | 95 |
| Hsp92II | CATG | 2 cut(s) | 280, 334 |
| HspAI | GCGC | 1 cut(s) | 126 |
| LpnPI | CCDG | 3 cut(s) | 76, 103, 136 |
| MaeI | CTAG | 1 cut(s) | 336 |
| MaeII | ACGT | 1 cut(s) | 341 |
| MboII | GAAGA | 3 cut(s) | 102, 250, 265 |
| MluCI | AATT | 3 cut(s) | 10, 106, 223 |
| MnlI | CCTC | 4 cut(s) | 25, 28, 109, 337 |
| MseI | TTAA | 1 cut(s) | 200 |
| MspR9I | CCNGG | 1 cut(s) | 91 |
| MvaI | CCWGG | 1 cut(s) | 91 |
| NlaIII | CATG | 2 cut(s) | 280, 334 |
| NlaIV | GGNNCC | 1 cut(s) | 240 |
| PagI | TCATGA | 1 cut(s) | 276 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 267 |
| PfeI | GAWTC | 3 cut(s) | 134, 297, 323 |
| Psp6I | CCWGG | 1 cut(s) | 89 |
| PspGI | CCWGG | 1 cut(s) | 89 |
| PspN4I | GGNNCC | 1 cut(s) | 240 |
| PspPI | GGNCC | 2 cut(s) | 41, 267 |
| SaqAI | TTAA | 1 cut(s) | 200 |
| Sau96I | GGNCC | 2 cut(s) | 41, 267 |
| ScrFI | CCNGG | 1 cut(s) | 91 |
| SetI | ASST | 4 cut(s) | 119, 125, 240, 344 |
| Sse9I | AATT | 3 cut(s) | 10, 106, 223 |
| SspI | AATATT | 1 cut(s) | 198 |
| SspMI | CTAG | 1 cut(s) | 336 |
| StyD4I | CCNGG | 1 cut(s) | 89 |
| StyI | CCWWGG | 1 cut(s) | 290 |
| TaiI | ACGT | 1 cut(s) | 344 |
| TaqI | TCGA | 2 cut(s) | 137, 221 |
| TasI | AATT | 3 cut(s) | 10, 106, 223 |
| TfiI | GAWTC | 3 cut(s) | 134, 297, 323 |
| Tru1I | TTAA | 1 cut(s) | 200 |
| Tru9I | TTAA | 1 cut(s) | 200 |
| TspDTI | ATGAA | 3 cut(s) | 42, 265, 293 |
| XapI | RAATTY | 2 cut(s) | 10, 223 |
| XmiI | GTMKAC | 2 cut(s) | 119, 248 |
| XspI | CTAG | 1 cut(s) | 336 |