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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 91 |
| AccI | GTMKAC | 1 cut(s) | 101 |
| AcsI | RAATTY | 1 cut(s) | 76 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 189 |
| AoxI | GGCC | 2 cut(s) | 120, 136 |
| ApoI | RAATTY | 1 cut(s) | 76 |
| AspS9I | GGNCC | 1 cut(s) | 120 |
| BanI | GGYRCC | 1 cut(s) | 91 |
| BbsI | GAAGAC | 1 cut(s) | 118 |
| BccI | CCATC | 1 cut(s) | 79 |
| BceAI | ACGGC | 1 cut(s) | 107 |
| BfaI | CTAG | 1 cut(s) | 189 |
| BmgT120I | GGNCC | 1 cut(s) | 120 |
| BmiI | GGNNCC | 1 cut(s) | 93 |
| BpiI | GAAGAC | 1 cut(s) | 118 |
| BsaJI | CCNNGG | 1 cut(s) | 143 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 189 |
| BseDI | CCNNGG | 1 cut(s) | 143 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 189 |
| BshFI | GGCC | 2 cut(s) | 122, 138 |
| BshNI | GGYRCC | 1 cut(s) | 91 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 189 |
| BsnI | GGCC | 2 cut(s) | 122, 138 |
| BspANI | GGCC | 2 cut(s) | 122, 138 |
| BspHI | TCATGA | 1 cut(s) | 129 |
| BspLI | GGNNCC | 1 cut(s) | 93 |
| BspT107I | GGYRCC | 1 cut(s) | 91 |
| BssECI | CCNNGG | 1 cut(s) | 143 |
| BssNAI | GTATAC | 1 cut(s) | 102 |
| BssT1I | CCWWGG | 1 cut(s) | 143 |
| Bst1107I | GTATAC | 1 cut(s) | 102 |
| BstV2I | GAAGAC | 1 cut(s) | 118 |
| BstZ17I | GTATAC | 1 cut(s) | 102 |
| BsuRI | GGCC | 2 cut(s) | 122, 138 |
| CciI | TCATGA | 1 cut(s) | 129 |
| Cfr13I | GGNCC | 1 cut(s) | 120 |
| CviAII | CATG | 2 cut(s) | 130, 184 |
| CviJI | RGCY | 3 cut(s) | 122, 138, 173 |
| CviKI_1 | RGCY | 3 cut(s) | 122, 138, 173 |
| Eco130I | CCWWGG | 1 cut(s) | 143 |
| Eco32I | GATATC | 1 cut(s) | 32 |
| EcoRI | GAATTC | 1 cut(s) | 76 |
| EcoRV | GATATC | 1 cut(s) | 32 |
| EcoT14I | CCWWGG | 1 cut(s) | 143 |
| ErhI | CCWWGG | 1 cut(s) | 143 |
| FaeI | CATG | 2 cut(s) | 133, 187 |
| FaiI | YATR | 4 cut(s) | 38, 102, 131, 185 |
| FatI | CATG | 2 cut(s) | 129, 183 |
| FblI | GTMKAC | 1 cut(s) | 101 |
| FspBI | CTAG | 1 cut(s) | 189 |
| HaeIII | GGCC | 2 cut(s) | 122, 138 |
| Hin1II | CATG | 2 cut(s) | 133, 187 |
| HinfI | GANTC | 2 cut(s) | 150, 176 |
| Hpy166II | GTNNAC | 1 cut(s) | 102 |
| Hpy188I | TCNGA | 1 cut(s) | 46 |
| Hpy188III | TCNNGA | 1 cut(s) | 130 |
| Hpy8I | GTNNAC | 1 cut(s) | 102 |
| HpyCH4IV | ACGT | 1 cut(s) | 194 |
| HpySE526I | ACGT | 1 cut(s) | 194 |
| Hsp92II | CATG | 2 cut(s) | 133, 187 |
| MaeI | CTAG | 1 cut(s) | 189 |
| MaeII | ACGT | 1 cut(s) | 194 |
| MboII | GAAGA | 2 cut(s) | 103, 118 |
| MluCI | AATT | 1 cut(s) | 76 |
| MnlI | CCTC | 1 cut(s) | 190 |
| MseI | TTAA | 1 cut(s) | 53 |
| NlaIII | CATG | 2 cut(s) | 133, 187 |
| NlaIV | GGNNCC | 1 cut(s) | 93 |
| PagI | TCATGA | 1 cut(s) | 129 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 120 |
| PfeI | GAWTC | 2 cut(s) | 150, 176 |
| PspN4I | GGNNCC | 1 cut(s) | 93 |
| PspPI | GGNCC | 1 cut(s) | 120 |
| SaqAI | TTAA | 1 cut(s) | 53 |
| Sau96I | GGNCC | 1 cut(s) | 120 |
| SetI | ASST | 2 cut(s) | 93, 197 |
| SgeI | CNNG | 6 cut(s) | 23, 39, 100, 131, 142, 156 |
| Sse9I | AATT | 1 cut(s) | 76 |
| SspI | AATATT | 1 cut(s) | 51 |
| SspMI | CTAG | 1 cut(s) | 189 |
| StyI | CCWWGG | 1 cut(s) | 143 |
| TaiI | ACGT | 1 cut(s) | 197 |
| TaqI | TCGA | 1 cut(s) | 74 |
| TasI | AATT | 1 cut(s) | 76 |
| TfiI | GAWTC | 2 cut(s) | 150, 176 |
| Tru1I | TTAA | 1 cut(s) | 53 |
| Tru9I | TTAA | 1 cut(s) | 53 |
| TspDTI | ATGAA | 2 cut(s) | 118, 146 |
| XapI | RAATTY | 1 cut(s) | 76 |
| XmiI | GTMKAC | 1 cut(s) | 101 |
| XspI | CTAG | 1 cut(s) | 189 |