pycom1654g00140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00001654
OG0001044
Physical Location & Seq
Forward (+)
106916 .. 108090
1175 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom1654g00140.6

Sequence Viewer

Length: 864 bp
ATGCCACTTATCCTTTTACGCAAAAGGATGATTCGAGAAGTTGGGAAAAACTTCTCCAGAAATGCCCTCTTCATGCTCTCCCATGATGTGACGGTTCCGGGAGCTAAATCATACAACCAATCCTTGGCTTTGTCCATTAAAGAGAATGGAAAAGCCTTCATCTTCAAAATGCTTCCGTCGACGTTGACGGGAGTCATGCTTGAACACACCACCTCGAATTCCTTCAGGTGCTTGTTCGGATCCTCCATGGCCAACCCATGGTATTTCGGAATATGGTGGAGCAAGCTTGACTTCAACTCGAACTCCTCTGTCTTCCCTTCGGCAGGTGGGGGATATTGGATACACAAAGGTGCTGCATTACCCAATCCCGAAGCCGCAAGCTGCTTGAGTGTCCGGGTGTCCACGGCCATGACTTGTGGTTCCTCAAAAATCCCTGTCAGATTCCGGGCTTGGTGGATTTGGTTCGTGTTGCTTCCTCGTCCGTCTCAAAGTCCTCTCAAAATCGCTGTCAAAGTTCGAAATATGCTCACGAATCGGTTGAGAACTCCGAGTCATACACTAGTACCTAAAACAAGGAAAACAAAACAAGCCAAGGGATTAGCAAAGTTGCTAATCCCCGGCAACGGCGCCAAAAATTTGATTGTAGTAAAGATCACTTGGAAACTGACTCAAAAACGTAAAACAAACACTAAAACAAATCAAAAGACTCAAAGAATACAAAACACTTATAAACACAAACTAAGACACTTTCTAACTAAATTAGACACTAAGGTAAAGGGGGATTGGCTAGCTAGAAGGTTCTTCTCCACACATGTCACACTTGCATACAATTTGATTGTCAGTTGGTCTTTCGATAAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

288

Amino Acids

32.65

Weight (kDa)

11.15

Isoelectric Point (pI)

26.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 729
AarI CACCTGC 1 cut(s) 314
Acc36I ACCTGC 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 626
AccB7I CCANNNNNTGG 2 cut(s) 124, 258
AccI GTMKAC 1 cut(s) 179
AciI CCGC 1 cut(s) 375
AclWI GGATC 2 cut(s) 234, 247
AcoI YGGCCR 2 cut(s) 249, 405
AcsI RAATTY 2 cut(s) 217, 634
AcuI CTGAAG 1 cut(s) 208
AcyI GRCGYC 1 cut(s) 627
AfaI GTAC 1 cut(s) 564
AfiI CCNNNNNNNGG 4 cut(s) 124, 258, 323, 623
AflIII ACRYGT 1 cut(s) 811
AgsI TTSAA 3 cut(s) 166, 203, 295
AhlI ACTAGT 1 cut(s) 559
AleI CACNNNNGTG 1 cut(s) 348
AluBI AGCT 4 cut(s) 104, 286, 381, 791
AluI AGCT 4 cut(s) 104, 286, 381, 791
Alw26I GTCTC 1 cut(s) 489
AlwI GGATC 2 cut(s) 234, 247
AoxI GGCC 2 cut(s) 249, 405
ApeKI GCWGC 2 cut(s) 353, 381
ApoI RAATTY 2 cut(s) 217, 634
ArsI GACNNNNNNTTYG 2 cut(s) 492, 524
Asp700I GAANNNNTTC 2 cut(s) 50, 221
AspLEI GCGC 1 cut(s) 629
AsuC2I CCSGG 4 cut(s) 99, 395, 446, 618
AsuII TTCGAA 1 cut(s) 517
AsuNHI GCTAGC 1 cut(s) 787
BaeI ACNNNNGTAYC 2 cut(s) 546, 579
BalI TGGCCA 1 cut(s) 251
BamHI GGATCC 1 cut(s) 239
BanI GGYRCC 1 cut(s) 626
BbsI GAAGAC 1 cut(s) 304
BbvI GCAGC 2 cut(s) 340, 368
BceAI ACGGC 2 cut(s) 420, 640
BciVI GTATCC 1 cut(s) 333
BcnI CCSGG 4 cut(s) 99, 395, 446, 618
BcoDI GTCTC 1 cut(s) 489
BcuI ACTAGT 1 cut(s) 559
BfaI CTAG 3 cut(s) 560, 788, 792
BfoI RGCGCY 1 cut(s) 630
BfuAI ACCTGC 1 cut(s) 314
BfuI GTATCC 1 cut(s) 333
BisI GCNGC 3 cut(s) 354, 375, 382
BlsI GCNGC 3 cut(s) 355, 376, 383
Bme1390I CCNGG 4 cut(s) 99, 395, 446, 618
BmiI GGNNCC 4 cut(s) 96, 241, 421, 628
BmrFI CCNGG 4 cut(s) 99, 395, 446, 618
BmtI GCTAGC 1 cut(s) 791
BoxI GACNNNNGTC 1 cut(s) 191
BpiI GAAGAC 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 40
Bpu14I TTCGAA 1 cut(s) 517
BpuEI CTTGAG 1 cut(s) 406
BpuMI CCSGG 4 cut(s) 99, 395, 446, 618
BsaHI GRCGYC 1 cut(s) 627
BsaJI CCNNGG 6 cut(s) 123, 246, 257, 402, 591, 616
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 258, 323, 623
BseDI CCNNGG 6 cut(s) 123, 246, 257, 402, 591, 616
BseGI GGATG 1 cut(s) 33
BseLI CCNNNNNNNGG 4 cut(s) 124, 258, 323, 623
BseRI GAGGAG 1 cut(s) 295
BseXI GCAGC 2 cut(s) 340, 368
BshFI GGCC 2 cut(s) 251, 407
BshNI GGYRCC 1 cut(s) 626
BsiSI CCGG 4 cut(s) 98, 394, 445, 618
BslI CCNNNNNNNGG 4 cut(s) 124, 258, 323, 623
BsmAI GTCTC 1 cut(s) 489
BsmBI CGTCTC 1 cut(s) 489
BsnI GGCC 2 cut(s) 251, 407
Bsp119I TTCGAA 1 cut(s) 517
Bsp143I GATC 2 cut(s) 239, 651
Bsp19I CCATGG 2 cut(s) 246, 257
BspACI CCGC 1 cut(s) 375
BspANI GGCC 2 cut(s) 251, 407
BspLI GGNNCC 4 cut(s) 96, 241, 421, 628
BspMI ACCTGC 1 cut(s) 314
BspOI GCTAGC 1 cut(s) 791
BspPI GGATC 2 cut(s) 234, 247
BspT104I TTCGAA 1 cut(s) 517
BspT107I GGYRCC 1 cut(s) 626
BssECI CCNNGG 6 cut(s) 123, 246, 257, 402, 591, 616
BssMI GATC 2 cut(s) 239, 651
BssNI GRCGYC 1 cut(s) 627
BssT1I CCWWGG 4 cut(s) 123, 246, 257, 591
Bst4CI ACNGT 1 cut(s) 94
Bst6I CTCTTC 1 cut(s) 74
BstACI GRCGYC 1 cut(s) 627
BstBI TTCGAA 1 cut(s) 517
BstC8I GCNNGC 3 cut(s) 284, 379, 789
BstDEI CTNAG 2 cut(s) 740, 768
BstDSI CCRYGG 3 cut(s) 246, 257, 402
BstENI CCTNNNNNAGG 1 cut(s) 321
BstF5I GGATG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 630
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 2 cut(s) 242, 654
BstMAI GTCTC 1 cut(s) 489
BstMBI GATC 2 cut(s) 239, 651
BstNSI RCATGY 1 cut(s) 815
BstPAI GACNNNNGTC 1 cut(s) 191
BstSCI CCNGG 4 cut(s) 97, 393, 444, 616
BstV1I GCAGC 2 cut(s) 340, 368
BstV2I GAAGAC 1 cut(s) 304
BstX2I RGATCY 1 cut(s) 239
BstYI RGATCY 1 cut(s) 239
BsuI GTATCC 1 cut(s) 333
BsuRI GGCC 2 cut(s) 251, 407
BtgI CCRYGG 3 cut(s) 246, 257, 402
BtsCI GGATG 1 cut(s) 33
BveI ACCTGC 1 cut(s) 314
Cac8I GCNNGC 3 cut(s) 284, 379, 789
CfoI GCGC 1 cut(s) 629
Csp6I GTAC 1 cut(s) 563
CviAII CATG 7 cut(s) 73, 83, 196, 247, 258, 409, 812
CviQI GTAC 1 cut(s) 563
DdeI CTNAG 2 cut(s) 740, 768
DinI GGCGCC 1 cut(s) 628
DpnI GATC 2 cut(s) 241, 653
DpnII GATC 2 cut(s) 239, 651
EaeI YGGCCR 2 cut(s) 249, 405
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco130I CCWWGG 4 cut(s) 123, 246, 257, 591
Eco57I CTGAAG 1 cut(s) 208
EcoNI CCTNNNNNAGG 1 cut(s) 321
EcoRI GAATTC 1 cut(s) 217
EcoT14I CCWWGG 4 cut(s) 123, 246, 257, 591
EgeI GGCGCC 1 cut(s) 628
EheI GGCGCC 1 cut(s) 628
ErhI CCWWGG 4 cut(s) 123, 246, 257, 591
Esp3I CGTCTC 1 cut(s) 489
FaeI CATG 7 cut(s) 76, 86, 199, 250, 261, 412, 815
FalI AAGNNNNNCTT 2 cut(s) 275, 307
FatI CATG 7 cut(s) 72, 82, 195, 246, 257, 408, 811
FblI GTMKAC 1 cut(s) 179
Fnu4HI GCNGC 3 cut(s) 354, 375, 382
FokI GGATG 1 cut(s) 40
Fsp4HI GCNGC 3 cut(s) 354, 375, 382
FspBI CTAG 3 cut(s) 560, 788, 792
GlaI GCGC 1 cut(s) 628
GluI GCNGC 3 cut(s) 354, 375, 382
GsuI CTGGAG 1 cut(s) 40
HaeII RGCGCY 1 cut(s) 630
HaeIII GGCC 2 cut(s) 251, 407
HapII CCGG 4 cut(s) 98, 394, 445, 618
HhaI GCGC 1 cut(s) 629
Hin1I GRCGYC 1 cut(s) 627
Hin1II CATG 7 cut(s) 76, 86, 199, 250, 261, 412, 815
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HincII GTYRAC 2 cut(s) 180, 186
HindII GTYRAC 2 cut(s) 180, 186
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 7 cut(s) 31, 192, 441, 532, 550, 667, 706
HpaII CCGG 4 cut(s) 98, 394, 445, 618
Hpy166II GTNNAC 3 cut(s) 180, 186, 402
Hpy188I TCNGA 4 cut(s) 239, 269, 440, 549
Hpy188III TCNNGA 4 cut(s) 35, 57, 368, 529
Hpy8I GTNNAC 3 cut(s) 180, 186, 402
Hpy99I CGWCG 2 cut(s) 181, 184
HpyAV CCTTC 4 cut(s) 166, 232, 327, 789
HpyCH4III ACNGT 1 cut(s) 94
HpyCH4IV ACGT 2 cut(s) 182, 676
HpyCH4V TGCA 2 cut(s) 356, 824
HpyF3I CTNAG 2 cut(s) 740, 768
HpySE526I ACGT 2 cut(s) 182, 676
Hsp92I GRCGYC 1 cut(s) 627
Hsp92II CATG 7 cut(s) 76, 86, 199, 250, 261, 412, 815
HspAI GCGC 1 cut(s) 627
KasI GGCGCC 1 cut(s) 626
Kzo9I GATC 2 cut(s) 239, 651
LmnI GCTCC 2 cut(s) 101, 279
LpnPI CCDG 8 cut(s) 70, 111, 211, 309, 407, 447, 458, 631
Lsp1109I GCAGC 2 cut(s) 340, 368
MaeI CTAG 3 cut(s) 560, 788, 792
MaeII ACGT 2 cut(s) 182, 676
MaeIII GTNAC 2 cut(s) 88, 814
MalI GATC 2 cut(s) 241, 653
MboI GATC 2 cut(s) 239, 651
MboII GAAGA 4 cut(s) 61, 154, 304, 793
MflI RGATCY 1 cut(s) 239
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 5 cut(s) 217, 634, 758, 829, 857
MluNI TGGCCA 1 cut(s) 251
Mly113I GGCGCC 1 cut(s) 627
MlyI GAGTC 4 cut(s) 201, 559, 661, 700
MnlI CCTC 7 cut(s) 77, 223, 253, 316, 433, 486, 504
Mox20I TGGCCA 1 cut(s) 251
MroXI GAANNNNTTC 2 cut(s) 50, 221
MscI TGGCCA 1 cut(s) 251
MseI TTAA 1 cut(s) 138
MslI CAYNNNNRTG 2 cut(s) 348, 407
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 4 cut(s) 98, 394, 445, 618
MspR9I CCNGG 4 cut(s) 99, 395, 446, 618
NarI GGCGCC 1 cut(s) 627
NciI CCSGG 4 cut(s) 99, 395, 446, 618
NcoI CCATGG 2 cut(s) 246, 257
NdeII GATC 2 cut(s) 239, 651
NheI GCTAGC 1 cut(s) 787
NlaIII CATG 7 cut(s) 76, 86, 199, 250, 261, 412, 815
NlaIV GGNNCC 4 cut(s) 96, 241, 421, 628
NmuCI GTSAC 2 cut(s) 88, 814
NspI RCATGY 1 cut(s) 815
NspV TTCGAA 1 cut(s) 517
OliI CACNNNNGTG 1 cut(s) 348
PaqCI CACCTGC 1 cut(s) 314
PciI ACATGT 1 cut(s) 811
PdmI GAANNNNTTC 2 cut(s) 50, 221
PfeI GAWTC 3 cut(s) 31, 441, 532
PflMI CCANNNNNTGG 2 cut(s) 124, 258
PfoI TCCNGGA 1 cut(s) 97
PkrI GCNGC 3 cut(s) 355, 376, 383
PleI GAGTC 4 cut(s) 200, 558, 661, 700
PluTI GGCGCC 1 cut(s) 630
PpsI GAGTC 4 cut(s) 200, 558, 661, 700
PscI ACATGT 1 cut(s) 811
PshAI GACNNNNGTC 1 cut(s) 191
PsiI TTATAA 1 cut(s) 729
PspN4I GGNNCC 4 cut(s) 96, 241, 421, 628
PsuI RGATCY 1 cut(s) 239
RsaI GTAC 1 cut(s) 564
RsaNI GTAC 1 cut(s) 563
RseI CAYNNNNRTG 2 cut(s) 348, 407
SalI GTCGAC 1 cut(s) 178
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 3 cut(s) 354, 375, 382
Sau3AI GATC 2 cut(s) 239, 651
SchI GAGTC 4 cut(s) 201, 559, 661, 700
ScrFI CCNGG 4 cut(s) 99, 395, 446, 618
SfoI GGCGCC 1 cut(s) 628
SfuI TTCGAA 1 cut(s) 517
SgrDI CGTCGACG 1 cut(s) 178
SmiMI CAYNNNNRTG 2 cut(s) 348, 407
SmlI CTYRAG 1 cut(s) 385
SmoI CTYRAG 1 cut(s) 385
SpeI ACTAGT 1 cut(s) 559
Sse9I AATT 5 cut(s) 217, 634, 758, 829, 857
SsiI CCGC 1 cut(s) 375
SspDI GGCGCC 1 cut(s) 626
SspMI CTAG 3 cut(s) 560, 788, 792
StyD4I CCNGG 4 cut(s) 97, 393, 444, 616
StyI CCWWGG 4 cut(s) 123, 246, 257, 591
TaaI ACNGT 1 cut(s) 94
TaiI ACGT 2 cut(s) 185, 679
TaqI TCGA 6 cut(s) 34, 179, 215, 299, 517, 852
TasI AATT 5 cut(s) 217, 634, 758, 829, 857
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 3 cut(s) 31, 441, 532
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TseFI GTSAC 2 cut(s) 88, 814
TseI GCWGC 2 cut(s) 353, 381
Tsp45I GTSAC 2 cut(s) 88, 814
TspDTI ATGAA 2 cut(s) 61, 148
TspGWI ACGGA 2 cut(s) 165, 471
Van91I CCANNNNNTGG 2 cut(s) 124, 258
XagI CCTNNNNNAGG 1 cut(s) 321
XapI RAATTY 2 cut(s) 217, 634
XceI RCATGY 1 cut(s) 815
XmiI GTMKAC 1 cut(s) 179
XmnI GAANNNNTTC 2 cut(s) 50, 221
XspI CTAG 3 cut(s) 560, 788, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.