pycom01g04350

Arogenate dehydratase prephenate dehydratase 2, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001044
Physical Location & Seq
Forward (+)
3263185 .. 3264706
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g04350.3

Sequence Viewer

Length: 1188 bp
ATGTTGTGTCCTGGCCAAAGTTTTATGATCATTTCTAACAACAATTGGTTGAAAATTGCATCCGCCTTGGTGATATCGAAAGCCTTGCAAACATATGGTTTATTTATGACTATTTCGGCCGCGTCCACATTGGCGTATTGGGGATCTTTGCTTTCGGCCAATATTGGGATTTTGATCGATCTTTTCCTCTCGGAATCTCGGATATTTGCCCCCAGGAATTTCTTTTTGTATTCTACATCCAAGCCGTTTAGAGCGATTCTAACAAATTCAACTTCAGGGAGAGGTACTCAGCACCAATTTCTGGCCGACTTGAACCTTGTCAAGTATTCCATTAGCGATTCATTTGAGGCTTGGGCCATCCTATGTTCATGGAATTTTTTGACCAGTTCCTCCCAACTCTGGACTAAGTTAGGTGGGAGGTTGATGTACCAGGCAAATGCTGAACCTGTTAGTGAGAAGTTGAATAAACGCAGTTTATGGAAATCGCTATTAACGTCTCCACATTGCGCAGTGAACCGAGCTACATGTTCTAATGAAGATAAGGATGATTCTCCAGCGAAAAGACTGAAGTCTGGGATCTTGAAGAACTTTGGGAACTTCGACCCCTTTTTTAAGGTCGAATCGATTATCCTTTGGACCTTGGTCATATTAACAGGTGTTTTTTCCATTCCCTGGTCGGGTCCGTTTGATCTACTGCTCGATCCCCCTCTTTTCTCCAAGTCAATTGGTTGGAGCCGAGCGGACCCTATCTTGACTTGTAGTGGAACATTCTTAAATGGAAGCTGTCGAGGTTGGCCAATGGGGCCATCTTCGAAGGCTTTACTGACCAATAGTTCAAGCAATCTGGTTTGCATATCACCATTGCTTTGTATTGCTTCAAGAAAATTATTCATTTTCTGGCCAATCGACTGTTCGACTTGCCGAGATTGTTCATCAAGTCGTCTTCGAAGAAATGACCTAGTCGGGGGATTAGAACCTTCCCCACCGTCTTCAACGCAATCTTCCACTATTCCTTCAATGAAAATGCCTGCAACAAACTTCTTTTTCTCAGTGTGTCGCAGTTGTAGCCTCGGGTGGCATAGTAATAAGGGGATTTTAATAGCACTCAAATTAAACCCTCTTTTTGACAATTGTAGTATAGATATAAGTAGGGATCGTTCTAGGCCGGGGATTAAGAGGGATTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

396

Amino Acids

43.66

Weight (kDa)

9.35

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 508
AccB7I CCANNNNNTGG 2 cut(s) 301, 672
AccBSI CCGCTC 1 cut(s) 740
AccII CGCG 1 cut(s) 122
AciI CCGC 3 cut(s) 63, 120, 740
AclWI GGATC 4 cut(s) 151, 584, 695, 1161
AcoI YGGCCR 6 cut(s) 13, 117, 156, 303, 794, 899
AcsI RAATTY 3 cut(s) 217, 265, 373
AcuI CTGAAG 2 cut(s) 258, 587
AfaI GTAC 2 cut(s) 286, 428
AfiI CCNNNNNNNGG 6 cut(s) 165, 301, 399, 672, 677, 964
AflIII ACRYGT 1 cut(s) 524
AgsI TTSAA 9 cut(s) 52, 270, 313, 463, 583, 837, 879, 993, 1017
AjnI CCWGG 4 cut(s) 10, 212, 429, 671
AjuI GAANNNNNNNTTGG 2 cut(s) 895, 927
AluBI AGCT 2 cut(s) 521, 783
AluI AGCT 2 cut(s) 521, 783
Alw26I GTCTC 1 cut(s) 501
AlwI GGATC 4 cut(s) 151, 584, 695, 1161
Ama87I CYCGRG 1 cut(s) 1070
AoxI GGCC 9 cut(s) 13, 117, 156, 303, 354, 794, 803, 899, 1163
ApoI RAATTY 3 cut(s) 217, 265, 373
AspLEI GCGC 1 cut(s) 509
AspS9I GGNCC 5 cut(s) 354, 636, 680, 742, 803
AsuC2I CCSGG 1 cut(s) 1167
AsuHPI GGTGA 2 cut(s) 82, 849
AsuII TTCGAA 2 cut(s) 812, 946
AvaI CYCGRG 1 cut(s) 1070
AvaII GGWCC 3 cut(s) 636, 680, 742
BalI TGGCCA 3 cut(s) 15, 796, 901
BbsI GAAGAC 2 cut(s) 935, 981
BccI CCATC 2 cut(s) 365, 814
BceAI ACGGC 1 cut(s) 229
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 4 cut(s) 12, 214, 431, 673
BclI TGATCA 1 cut(s) 27
BcnI CCSGG 1 cut(s) 1167
BcoDI GTCTC 1 cut(s) 501
BfaI CTAG 2 cut(s) 959, 1161
BglI GCCNNNNNGGC 1 cut(s) 802
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
Bme1390I CCNGG 5 cut(s) 12, 214, 431, 673, 1167
Bme18I GGWCC 3 cut(s) 636, 680, 742
BmeT110I CYCGRG 1 cut(s) 1070
BmgT120I GGNCC 5 cut(s) 354, 636, 680, 742, 803
BmiI GGNNCC 4 cut(s) 681, 734, 744, 804
BmrFI CCNGG 5 cut(s) 12, 214, 431, 673, 1167
BmsI GCATC 1 cut(s) 68
BoxI GACNNNNGTC 2 cut(s) 568, 641
BpiI GAAGAC 2 cut(s) 935, 981
BplI GAGNNNNNCTC 2 cut(s) 271, 303
BpmI CTGGAG 1 cut(s) 537
Bpu14I TTCGAA 2 cut(s) 812, 946
BpuMI CCSGG 1 cut(s) 1167
Bsa29I ATCGAT 2 cut(s) 177, 623
BsaBI GATNNNNATC 1 cut(s) 173
BsaJI CCNNGG 6 cut(s) 66, 212, 639, 671, 1069, 1166
Bsc4I CCNNNNNNNGG 6 cut(s) 165, 301, 399, 672, 677, 964
Bse1I ACTGG 1 cut(s) 384
Bse3DI GCAATG 2 cut(s) 502, 860
Bse8I GATNNNNATC 1 cut(s) 173
BseBI CCWGG 4 cut(s) 12, 214, 431, 673
BseCI ATCGAT 2 cut(s) 177, 623
BseDI CCNNGG 6 cut(s) 66, 212, 639, 671, 1069, 1166
BseGI GGATG 4 cut(s) 59, 236, 357, 550
BseJI GATNNNNATC 1 cut(s) 173
BseLI CCNNNNNNNGG 6 cut(s) 165, 301, 399, 672, 677, 964
BseMI GCAATG 2 cut(s) 502, 860
BseMII CTCAG 2 cut(s) 302, 1062
BseNI ACTGG 1 cut(s) 384
BseX3I CGGCCG 1 cut(s) 117
Bsh1236I CGCG 1 cut(s) 122
Bsh1285I CGRYCG 1 cut(s) 120
BshFI GGCC 9 cut(s) 15, 119, 158, 305, 356, 796, 805, 901, 1165
BshVI ATCGAT 2 cut(s) 177, 623
BsiEI CGRYCG 1 cut(s) 120
BsiHKCI CYCGRG 1 cut(s) 1070
BsiSI CCGG 1 cut(s) 1166
BslI CCNNNNNNNGG 6 cut(s) 165, 301, 399, 672, 677, 964
BsmAI GTCTC 1 cut(s) 501
BsmBI CGTCTC 1 cut(s) 501
BsnI GGCC 9 cut(s) 15, 119, 158, 305, 356, 796, 805, 901, 1165
BsoBI CYCGRG 1 cut(s) 1070
Bsp119I TTCGAA 2 cut(s) 812, 946
Bsp143I GATC 8 cut(s) 27, 143, 174, 178, 576, 688, 700, 1153
BspACI CCGC 3 cut(s) 63, 120, 740
BspANI GGCC 9 cut(s) 15, 119, 158, 305, 356, 796, 805, 901, 1165
BspCNI CTCAG 2 cut(s) 301, 1061
BspDI ATCGAT 2 cut(s) 177, 623
BspFNI CGCG 1 cut(s) 122
BspLI GGNNCC 4 cut(s) 681, 734, 744, 804
BspPI GGATC 4 cut(s) 151, 584, 695, 1161
BspT104I TTCGAA 2 cut(s) 812, 946
BsrBI CCGCTC 1 cut(s) 740
BsrDI GCAATG 2 cut(s) 502, 860
BsrI ACTGG 1 cut(s) 384
BssECI CCNNGG 6 cut(s) 66, 212, 639, 671, 1069, 1166
BssMI GATC 8 cut(s) 27, 143, 174, 178, 576, 688, 700, 1153
BssT1I CCWWGG 2 cut(s) 66, 639
Bst2UI CCWGG 4 cut(s) 12, 214, 431, 673
Bst4CI ACNGT 2 cut(s) 911, 987
BstBI TTCGAA 2 cut(s) 812, 946
BstC8I GCNNGC 1 cut(s) 1029
BstDEI CTNAG 3 cut(s) 288, 405, 1048
BstF5I GGATG 4 cut(s) 59, 236, 357, 550
BstFNI CGCG 1 cut(s) 122
BstHHI GCGC 1 cut(s) 509
BstKTI GATC 8 cut(s) 30, 146, 177, 181, 579, 691, 703, 1156
BstMAI GTCTC 1 cut(s) 501
BstMBI GATC 8 cut(s) 27, 143, 174, 178, 576, 688, 700, 1153
BstMCI CGRYCG 1 cut(s) 120
BstMWI GCNNNNNNNGC 2 cut(s) 802, 1065
BstNI CCWGG 4 cut(s) 12, 214, 431, 673
BstNSI RCATGY 1 cut(s) 528
BstPAI GACNNNNGTC 2 cut(s) 568, 641
BstSCI CCNGG 5 cut(s) 10, 212, 429, 671, 1165
BstUI CGCG 1 cut(s) 122
BstV2I GAAGAC 2 cut(s) 935, 981
BstX2I RGATCY 2 cut(s) 143, 576
BstYI RGATCY 2 cut(s) 143, 576
BstZI CGGCCG 1 cut(s) 117
Bsu15I ATCGAT 2 cut(s) 177, 623
BsuRI GGCC 9 cut(s) 15, 119, 158, 305, 356, 796, 805, 901, 1165
BsuTUI ATCGAT 2 cut(s) 177, 623
BtsCI GGATG 4 cut(s) 59, 236, 357, 550
BtsI GCAGTG 1 cut(s) 516
BtsIMutI CAGTG 2 cut(s) 516, 1056
Cac8I GCNNGC 1 cut(s) 1029
CfoI GCGC 1 cut(s) 509
Cfr13I GGNCC 5 cut(s) 354, 636, 680, 742, 803
ClaI ATCGAT 2 cut(s) 177, 623
CseI GACGC 1 cut(s) 111
Csp6I GTAC 2 cut(s) 285, 427
CviAII CATG 2 cut(s) 369, 525
CviQI GTAC 2 cut(s) 285, 427
DdeI CTNAG 3 cut(s) 288, 405, 1048
DpnI GATC 8 cut(s) 29, 145, 176, 180, 578, 690, 702, 1155
DpnII GATC 8 cut(s) 27, 143, 174, 178, 576, 688, 700, 1153
EaeI YGGCCR 6 cut(s) 13, 117, 156, 303, 794, 899
EagI CGGCCG 1 cut(s) 117
EciI GGCGGA 1 cut(s) 52
EclXI CGGCCG 1 cut(s) 117
Eco130I CCWWGG 2 cut(s) 66, 639
Eco32I GATATC 1 cut(s) 75
Eco47I GGWCC 3 cut(s) 636, 680, 742
Eco52I CGGCCG 1 cut(s) 117
Eco57I CTGAAG 2 cut(s) 258, 587
Eco88I CYCGRG 1 cut(s) 1070
EcoRII CCWGG 4 cut(s) 10, 212, 429, 671
EcoRV GATATC 1 cut(s) 75
EcoT14I CCWWGG 2 cut(s) 66, 639
ErhI CCWWGG 2 cut(s) 66, 639
Esp3I CGTCTC 1 cut(s) 501
FaeI CATG 2 cut(s) 372, 528
FatI CATG 2 cut(s) 368, 524
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 1 cut(s) 27
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 4 cut(s) 46, 223, 344, 557
Fsp4HI GCNGC 1 cut(s) 120
FspBI CTAG 2 cut(s) 959, 1161
FspI TGCGCA 1 cut(s) 508
GlaI GCGC 1 cut(s) 508
GluI GCNGC 1 cut(s) 120
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 9 cut(s) 15, 119, 158, 305, 356, 796, 805, 901, 1165
HapII CCGG 1 cut(s) 1166
HgaI GACGC 1 cut(s) 111
HhaI GCGC 1 cut(s) 509
Hin1II CATG 2 cut(s) 372, 528
Hin6I GCGC 1 cut(s) 507
HinP1I GCGC 1 cut(s) 507
HinfI GANTC 5 cut(s) 194, 256, 338, 548, 620
HpaII CCGG 1 cut(s) 1166
HphI GGTGA 2 cut(s) 82, 849
Hpy166II GTNNAC 2 cut(s) 126, 514
Hpy188I TCNGA 2 cut(s) 193, 201
Hpy188III TCNNGA 4 cut(s) 400, 580, 751, 879
Hpy8I GTNNAC 2 cut(s) 126, 514
HpyAV CCTTC 3 cut(s) 808, 987, 1023
HpyCH4III ACNGT 2 cut(s) 911, 987
HpyCH4IV ACGT 1 cut(s) 494
HpyCH4V TGCA 4 cut(s) 59, 88, 852, 1031
HpyF10VI GCNNNNNNNGC 2 cut(s) 802, 1065
HpyF3I CTNAG 3 cut(s) 288, 405, 1048
HpySE526I ACGT 1 cut(s) 494
Hsp92II CATG 2 cut(s) 372, 528
HspAI GCGC 1 cut(s) 507
Ksp22I TGATCA 1 cut(s) 27
Kzo9I GATC 8 cut(s) 27, 143, 174, 178, 576, 688, 700, 1153
LmnI GCTCC 1 cut(s) 732
LweI GCATC 1 cut(s) 68
MaeI CTAG 2 cut(s) 959, 1161
MaeII ACGT 1 cut(s) 494
MalI GATC 8 cut(s) 29, 145, 176, 180, 578, 690, 702, 1155
MbiI CCGCTC 1 cut(s) 740
MboI GATC 8 cut(s) 27, 143, 174, 178, 576, 688, 700, 1153
MboII GAAGA 7 cut(s) 548, 595, 801, 935, 960, 981, 993
MfeI CAATTG 3 cut(s) 43, 723, 1129
MflI RGATCY 2 cut(s) 143, 576
MlsI TGGCCA 3 cut(s) 15, 796, 901
MluNI TGGCCA 3 cut(s) 15, 796, 901
MmeI TCCRAC 1 cut(s) 710
Mox20I TGGCCA 3 cut(s) 15, 796, 901
MscI TGGCCA 3 cut(s) 15, 796, 901
MseI TTAA 7 cut(s) 491, 612, 650, 773, 1097, 1112, 1173
Msp20I TGGCCA 3 cut(s) 15, 796, 901
MspI CCGG 1 cut(s) 1166
MspR9I CCNGG 5 cut(s) 12, 214, 431, 673, 1167
MunI CAATTG 3 cut(s) 43, 723, 1129
MvaI CCWGG 4 cut(s) 12, 214, 431, 673
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 2 cut(s) 802, 1065
NciI CCSGG 1 cut(s) 1167
NdeI CATATG 1 cut(s) 94
NdeII GATC 8 cut(s) 27, 143, 174, 178, 576, 688, 700, 1153
NlaIII CATG 2 cut(s) 372, 528
NlaIV GGNNCC 4 cut(s) 681, 734, 744, 804
NmeAIII GCCGAG 2 cut(s) 761, 947
NsbI TGCGCA 1 cut(s) 508
NspI RCATGY 1 cut(s) 528
NspV TTCGAA 2 cut(s) 812, 946
PciI ACATGT 1 cut(s) 524
PcsI WCGNNNNNNNCGW 1 cut(s) 491
PfeI GAWTC 5 cut(s) 194, 256, 338, 548, 620
PflFI GACNNNGTC 1 cut(s) 959
PflMI CCANNNNNTGG 2 cut(s) 301, 672
PkrI GCNGC 1 cut(s) 121
PscI ACATGT 1 cut(s) 524
PshAI GACNNNNGTC 2 cut(s) 568, 641
Psp6I CCWGG 4 cut(s) 10, 212, 429, 671
PspGI CCWGG 4 cut(s) 10, 212, 429, 671
PspN4I GGNNCC 4 cut(s) 681, 734, 744, 804
PspPI GGNCC 5 cut(s) 354, 636, 680, 742, 803
PsrI GAACNNNNNNTAC 2 cut(s) 1141, 1173
PsuI RGATCY 2 cut(s) 143, 576
PsyI GACNNNGTC 1 cut(s) 959
RsaI GTAC 2 cut(s) 286, 428
RsaNI GTAC 2 cut(s) 285, 427
SaqAI TTAA 7 cut(s) 491, 612, 650, 773, 1097, 1112, 1173
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 8 cut(s) 27, 143, 174, 178, 576, 688, 700, 1153
Sau96I GGNCC 5 cut(s) 354, 636, 680, 742, 803
ScrFI CCNGG 5 cut(s) 12, 214, 431, 673, 1167
SfaNI GCATC 1 cut(s) 68
SfiI GGCCNNNNNGGCC 1 cut(s) 802
SfuI TTCGAA 2 cut(s) 812, 946
SinI GGWCC 3 cut(s) 636, 680, 742
SsiI CCGC 3 cut(s) 63, 120, 740
SspI AATATT 1 cut(s) 163
SspMI CTAG 2 cut(s) 959, 1161
StyD4I CCNGG 5 cut(s) 10, 212, 429, 671, 1165
StyI CCWWGG 2 cut(s) 66, 639
TaaI ACNGT 2 cut(s) 911, 987
TaiI ACGT 1 cut(s) 497
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 5 cut(s) 194, 256, 338, 548, 620
Tru1I TTAA 7 cut(s) 491, 612, 650, 773, 1097, 1112, 1173
Tru9I TTAA 7 cut(s) 491, 612, 650, 773, 1097, 1112, 1173
TscAI CASTG 2 cut(s) 516, 1056
TspDTI ATGAA 6 cut(s) 330, 357, 549, 880, 921, 1034
TspGWI ACGGA 1 cut(s) 672
TspRI CASTG 2 cut(s) 516, 1056
Tth111I GACNNNGTC 1 cut(s) 959
Van91I CCANNNNNTGG 2 cut(s) 301, 672
VpaK11BI GGWCC 3 cut(s) 636, 680, 742
XapI RAATTY 3 cut(s) 217, 265, 373
XceI RCATGY 1 cut(s) 528
XspI CTAG 2 cut(s) 959, 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.