pycom01g00370

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001044
Physical Location & Seq
Forward (+)
461665 .. 463002
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g00370.7

Sequence Viewer

Length: 1029 bp
ATGTACTTGCTGCCGTGGGGGTCCTCTTTGGCCTACTTCTTCGTATTGTTGAGCGTTCAATGCTCGGTTAGCAATCAAAACCTTGGCATCCCTGGGTGTCTTGTCCACCAATGCTCCACCCGCCGAGAAGTATTGCAGAAGCAACTCCTCCTTCATCTGATGCTGTGGACACGAAACTCACCTTCTTCTTGTTGGATTCCACTTATCCTTTTTCGTAAAAGGATGATTCGAGAAGTTGGGAAAAACTTCTCCAGATATGCCCTCTTCATGCTCTCCCAAGATGTGACGGTTCCGGGAGCTAAGTCGTACAACCAATCCTTGGCTTTGTCCATTAAAGAGAATGGAAAAGCCTTCATCTTCAAAATACTTCCGTCGATGTTGACGGGAGTCATGCTTGAACACACTACCTCAAATTCCTTCAGGTGCTTTTTCGGATCCTCCATGGACAATCCATGGTACTTCGGAATGTGGTGGAGCAAGCTTGACTTCAACTCAAACTCCTCGGTCTTGCCTTTGGCCGGTGGGGGATATTGGATACACAAGGTGTCCGGTTGTCCAAGGCCATGCCTTAGGGTTCTTCAACTACCCCCTTGGGTGCCTTGGGCTCCTCCTTCTCTTCTGGGACTTGTTCTTCAAGCTCAGGTTCTGGGCTTGATAGATTTGACTCGTGGTACTTCTTCCTCCGTCTCAAAGTCCTCTCAAAATCACTGTCAAAGTCCGAGATATCCTCACGAATCGGTGGAGAACTCCGAGTCATACACTAGGATTAGCAAAGTTGCCAATCCCCGGCAACGGCGCCAAAATTTAATGCGTGACTTATGTGACACACAAATTAAACCCTCTTGTGACAATTGTAGTATATTGCAAAATCACTTGGAAACTGACTCAAAAACACAAATCAAAAGACTCAAACAGAAACTAGAACTTGACTTGGACGAATTCGAAAGTGTTTATGACTCCAAATGCTTAAAGACACAAAATAAGATAGATTGTAATGATTACGATTTTAACAAAATATGGGGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

343

Amino Acids

39.08

Weight (kDa)

9.13

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 595, 795
AccB7I CCANNNNNTGG 1 cut(s) 319
AciI CCGC 1 cut(s) 121
AclWI GGATC 2 cut(s) 429, 442
AcoI YGGCCR 1 cut(s) 516
AcsI RAATTY 3 cut(s) 412, 802, 938
AcuI CTGAAG 1 cut(s) 403
AcyI GRCGYC 1 cut(s) 796
AdeI CACNNNGTG 1 cut(s) 544
AfaI GTAC 4 cut(s) 5, 308, 458, 673
AfiI CCNNNNNNNGG 4 cut(s) 319, 518, 786, 792
AgsI TTSAA 6 cut(s) 59, 361, 398, 490, 581, 635
AjnI CCWGG 1 cut(s) 91
AluBI AGCT 3 cut(s) 299, 481, 638
AluI AGCT 3 cut(s) 299, 481, 638
Alw26I GTCTC 1 cut(s) 691
AlwI GGATC 2 cut(s) 429, 442
AlwNI CAGNNNCTG 1 cut(s) 646
AoxI GGCC 3 cut(s) 30, 516, 560
ApeKI GCWGC 1 cut(s) 10
ApoI RAATTY 3 cut(s) 412, 802, 938
ArsI GACNNNNNNTTYG 2 cut(s) 694, 726
Asp700I GAANNNNTTC 1 cut(s) 245
AspLEI GCGC 1 cut(s) 798
AspS9I GGNCC 1 cut(s) 21
AsuC2I CCSGG 2 cut(s) 294, 787
AsuHPI GGTGA 1 cut(s) 171
AsuII TTCGAA 1 cut(s) 942
AvaII GGWCC 1 cut(s) 21
AxyI CCTNAGG 1 cut(s) 569
BamHI GGATCC 1 cut(s) 434
BanI GGYRCC 2 cut(s) 595, 795
BanII GRGCYC 1 cut(s) 607
BauI CACGAG 1 cut(s) 666
BceAI ACGGC 1 cut(s) 809
BciT130I CCWGG 1 cut(s) 93
BciVI GTATCC 1 cut(s) 528
BcnI CCSGG 2 cut(s) 294, 787
BcoDI GTCTC 1 cut(s) 691
BfaI CTAG 2 cut(s) 762, 920
BfoI RGCGCY 1 cut(s) 799
BfuI GTATCC 1 cut(s) 528
BisI GCNGC 1 cut(s) 11
BlsI GCNGC 1 cut(s) 12
Bme1390I CCNGG 3 cut(s) 93, 294, 787
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 21
BmiI GGNNCC 6 cut(s) 22, 291, 436, 597, 606, 797
BmrFI CCNGG 3 cut(s) 93, 294, 787
BmsI GCATC 2 cut(s) 96, 150
BoxI GACNNNNGTC 1 cut(s) 386
BplI GAGNNNNNCTC 2 cut(s) 712, 744
BpmI CTGGAG 1 cut(s) 235
Bpu10I CCTNAGC 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 942
BpuMI CCSGG 2 cut(s) 294, 787
BsaHI GRCGYC 1 cut(s) 796
BsaWI WCCGGW 1 cut(s) 548
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 482, 512
Bsc4I CCNNNNNNNGG 4 cut(s) 319, 518, 786, 792
Bse118I RCCGGY 1 cut(s) 518
Bse21I CCTNAGG 1 cut(s) 569
BseBI CCWGG 1 cut(s) 93
BseGI GGATG 2 cut(s) 87, 228
BseLI CCNNNNNNNGG 4 cut(s) 319, 518, 786, 792
BseMII CTCAG 1 cut(s) 653
BseRI GAGGAG 3 cut(s) 137, 490, 597
BshFI GGCC 3 cut(s) 32, 518, 562
BshNI GGYRCC 2 cut(s) 595, 795
BsiSI CCGG 4 cut(s) 293, 519, 549, 787
BslFI GGGAC 1 cut(s) 636
BslI CCNNNNNNNGG 4 cut(s) 319, 518, 786, 792
BsmAI GTCTC 1 cut(s) 691
BsmBI CGTCTC 1 cut(s) 691
BsmFI GGGAC 1 cut(s) 636
BsnI GGCC 3 cut(s) 32, 518, 562
Bsp119I TTCGAA 1 cut(s) 942
Bsp1286I GDGCHC 1 cut(s) 607
Bsp143I GATC 1 cut(s) 434
Bsp19I CCATGG 2 cut(s) 441, 452
BspACI CCGC 1 cut(s) 121
BspANI GGCC 3 cut(s) 32, 518, 562
BspCNI CTCAG 1 cut(s) 652
BspLI GGNNCC 6 cut(s) 22, 291, 436, 597, 606, 797
BspPI GGATC 2 cut(s) 429, 442
BspT104I TTCGAA 1 cut(s) 942
BspT107I GGYRCC 2 cut(s) 595, 795
BsrFI RCCGGY 1 cut(s) 518
BssAI RCCGGY 1 cut(s) 518
BssMI GATC 1 cut(s) 434
BssNI GRCGYC 1 cut(s) 796
BssSI CACGAG 1 cut(s) 666
BssT1I CCWWGG 7 cut(s) 82, 318, 441, 452, 557, 590, 599
Bst2BI CACGAG 1 cut(s) 666
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 2 cut(s) 289, 710
Bst6I CTCTTC 2 cut(s) 269, 621
BstACI GRCGYC 1 cut(s) 796
BstBI TTCGAA 1 cut(s) 942
BstC8I GCNNGC 1 cut(s) 479
BstDEI CTNAG 3 cut(s) 300, 569, 639
BstDSI CCRYGG 3 cut(s) 14, 441, 452
BstF5I GGATG 2 cut(s) 87, 228
BstH2I RGCGCY 1 cut(s) 799
BstHHI GCGC 1 cut(s) 798
BstKTI GATC 1 cut(s) 437
BstMAI GTCTC 1 cut(s) 691
BstMBI GATC 1 cut(s) 434
BstMWI GCNNNNNNNGC 3 cut(s) 60, 69, 120
BstNI CCWGG 1 cut(s) 93
BstPAI GACNNNNGTC 1 cut(s) 386
BstSCI CCNGG 3 cut(s) 91, 292, 785
BstX2I RGATCY 1 cut(s) 434
BstYI RGATCY 1 cut(s) 434
Bsu36I CCTNAGG 1 cut(s) 569
BsuI GTATCC 1 cut(s) 528
BsuRI GGCC 3 cut(s) 32, 518, 562
BtgI CCRYGG 3 cut(s) 14, 441, 452
BtsCI GGATG 2 cut(s) 87, 228
BtsIMutI CAGTG 1 cut(s) 706
Cac8I GCNNGC 1 cut(s) 479
CaiI CAGNNNCTG 1 cut(s) 646
CfoI GCGC 1 cut(s) 798
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 1 cut(s) 21
Csp6I GTAC 4 cut(s) 4, 307, 457, 672
CviAII CATG 5 cut(s) 268, 391, 442, 453, 564
CviQI GTAC 4 cut(s) 4, 307, 457, 672
DdeI CTNAG 3 cut(s) 300, 569, 639
DinI GGCGCC 1 cut(s) 797
DpnI GATC 1 cut(s) 436
DpnII GATC 1 cut(s) 434
DraIII CACNNNGTG 1 cut(s) 544
EaeI YGGCCR 1 cut(s) 516
Eam1104I CTCTTC 2 cut(s) 269, 621
EarI CTCTTC 2 cut(s) 269, 621
Eco130I CCWWGG 7 cut(s) 82, 318, 441, 452, 557, 590, 599
Eco24I GRGCYC 1 cut(s) 607
Eco32I GATATC 1 cut(s) 725
Eco47I GGWCC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 403
Eco81I CCTNAGG 1 cut(s) 569
EcoO109I RGGNCCY 1 cut(s) 21
EcoRI GAATTC 1 cut(s) 938
EcoRII CCWGG 1 cut(s) 91
EcoRV GATATC 1 cut(s) 725
EcoT14I CCWWGG 7 cut(s) 82, 318, 441, 452, 557, 590, 599
EcoT38I GRGCYC 1 cut(s) 607
EgeI GGCGCC 1 cut(s) 797
EheI GGCGCC 1 cut(s) 797
ErhI CCWWGG 7 cut(s) 82, 318, 441, 452, 557, 590, 599
Esp3I CGTCTC 1 cut(s) 691
FaeI CATG 5 cut(s) 271, 394, 445, 456, 567
FalI AAGNNNNNCTT 2 cut(s) 470, 502
FaqI GGGAC 1 cut(s) 636
FatI CATG 5 cut(s) 267, 390, 441, 452, 563
FauI CCCGC 1 cut(s) 128
Fnu4HI GCNGC 1 cut(s) 11
FokI GGATG 2 cut(s) 74, 235
FriOI GRGCYC 1 cut(s) 607
Fsp4HI GCNGC 1 cut(s) 11
FspBI CTAG 2 cut(s) 762, 920
GlaI GCGC 1 cut(s) 797
GluI GCNGC 1 cut(s) 11
GsuI CTGGAG 1 cut(s) 235
HaeII RGCGCY 1 cut(s) 799
HaeIII GGCC 3 cut(s) 32, 518, 562
HapII CCGG 4 cut(s) 293, 519, 549, 787
HhaI GCGC 1 cut(s) 798
Hin1I GRCGYC 1 cut(s) 796
Hin1II CATG 5 cut(s) 271, 394, 445, 456, 567
Hin6I GCGC 1 cut(s) 796
HinP1I GCGC 1 cut(s) 796
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 9 cut(s) 196, 226, 387, 664, 734, 752, 884, 906, 956
HpaII CCGG 4 cut(s) 293, 519, 549, 787
HphI GGTGA 1 cut(s) 171
Hpy166II GTNNAC 3 cut(s) 106, 168, 381
Hpy188I TCNGA 5 cut(s) 159, 434, 464, 720, 751
Hpy188III TCNNGA 3 cut(s) 230, 252, 731
Hpy8I GTNNAC 3 cut(s) 106, 168, 381
Hpy99I CGWCG 1 cut(s) 376
HpyAV CCTTC 5 cut(s) 161, 192, 361, 427, 621
HpyCH4III ACNGT 2 cut(s) 289, 710
HpyCH4V TGCA 2 cut(s) 136, 865
HpyF10VI GCNNNNNNNGC 3 cut(s) 60, 69, 120
HpyF3I CTNAG 3 cut(s) 300, 569, 639
Hsp92I GRCGYC 1 cut(s) 796
Hsp92II CATG 5 cut(s) 271, 394, 445, 456, 567
HspAI GCGC 1 cut(s) 796
KasI GGCGCC 1 cut(s) 795
Kzo9I GATC 1 cut(s) 434
LmnI GCTCC 4 cut(s) 119, 296, 474, 610
LweI GCATC 2 cut(s) 96, 150
MaeI CTAG 2 cut(s) 762, 920
MaeIII GTNAC 4 cut(s) 283, 812, 821, 845
MalI GATC 1 cut(s) 436
MboI GATC 1 cut(s) 434
MboII GAAGA 8 cut(s) 31, 177, 256, 349, 569, 608, 623, 669
MfeI CAATTG 1 cut(s) 850
MflI RGATCY 1 cut(s) 434
MhlI GDGCHC 1 cut(s) 607
MluCI AATT 5 cut(s) 412, 802, 831, 850, 938
Mly113I GGCGCC 1 cut(s) 796
MlyI GAGTC 6 cut(s) 396, 658, 761, 878, 900, 950
MmeI TCCRAC 1 cut(s) 173
MroXI GAANNNNTTC 1 cut(s) 245
MseI TTAA 5 cut(s) 333, 806, 834, 968, 1008
MspI CCGG 4 cut(s) 293, 519, 549, 787
MspR9I CCNGG 3 cut(s) 93, 294, 787
MunI CAATTG 1 cut(s) 850
MvaI CCWGG 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 60, 69, 120
NarI GGCGCC 1 cut(s) 796
NciI CCSGG 2 cut(s) 294, 787
NcoI CCATGG 2 cut(s) 441, 452
NdeII GATC 1 cut(s) 434
NlaIII CATG 5 cut(s) 271, 394, 445, 456, 567
NlaIV GGNNCC 6 cut(s) 22, 291, 436, 597, 606, 797
NmeAIII GCCGAG 1 cut(s) 149
NmuCI GTSAC 4 cut(s) 283, 812, 821, 845
NspV TTCGAA 1 cut(s) 942
PasI CCCWGGG 1 cut(s) 92
PdmI GAANNNNTTC 1 cut(s) 245
PfeI GAWTC 3 cut(s) 196, 226, 734
PflMI CCANNNNNTGG 1 cut(s) 319
PfoI TCCNGGA 1 cut(s) 292
PkrI GCNGC 1 cut(s) 12
PleI GAGTC 6 cut(s) 395, 658, 760, 878, 900, 950
PluTI GGCGCC 1 cut(s) 799
PpsI GAGTC 6 cut(s) 395, 658, 760, 878, 900, 950
PpuMI RGGWCCY 1 cut(s) 21
PshAI GACNNNNGTC 1 cut(s) 386
Psp5II RGGWCCY 1 cut(s) 21
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 6 cut(s) 22, 291, 436, 597, 606, 797
PspPI GGNCC 1 cut(s) 21
PspPPI RGGWCCY 1 cut(s) 21
PstNI CAGNNNCTG 1 cut(s) 646
PsuI RGATCY 1 cut(s) 434
RsaI GTAC 4 cut(s) 5, 308, 458, 673
RsaNI GTAC 4 cut(s) 4, 307, 457, 672
SaqAI TTAA 5 cut(s) 333, 806, 834, 968, 1008
SatI GCNGC 1 cut(s) 11
Sau3AI GATC 1 cut(s) 434
Sau96I GGNCC 1 cut(s) 21
SchI GAGTC 6 cut(s) 396, 658, 761, 878, 900, 950
ScrFI CCNGG 3 cut(s) 93, 294, 787
SduI GDGCHC 1 cut(s) 607
SetI ASST 9 cut(s) 84, 184, 301, 410, 425, 483, 546, 640, 645
SfaNI GCATC 2 cut(s) 96, 150
SfoI GGCGCC 1 cut(s) 797
SfuI TTCGAA 1 cut(s) 942
SinI GGWCC 1 cut(s) 21
Sse9I AATT 5 cut(s) 412, 802, 831, 850, 938
SsiI CCGC 1 cut(s) 121
SspDI GGCGCC 1 cut(s) 795
SspMI CTAG 2 cut(s) 762, 920
StyD4I CCNGG 3 cut(s) 91, 292, 785
StyI CCWWGG 7 cut(s) 82, 318, 441, 452, 557, 590, 599
TaaI ACNGT 2 cut(s) 289, 710
TaqI TCGA 3 cut(s) 229, 374, 942
TaqII GACCGA 1 cut(s) 493
TasI AATT 5 cut(s) 412, 802, 831, 850, 938
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 3 cut(s) 196, 226, 734
Tru1I TTAA 5 cut(s) 333, 806, 834, 968, 1008
Tru9I TTAA 5 cut(s) 333, 806, 834, 968, 1008
TscAI CASTG 1 cut(s) 713
TseFI GTSAC 4 cut(s) 283, 812, 821, 845
TseI GCWGC 1 cut(s) 10
Tsp45I GTSAC 4 cut(s) 283, 812, 821, 845
TspDTI ATGAA 3 cut(s) 143, 256, 343
TspGWI ACGGA 2 cut(s) 360, 673
TspRI CASTG 1 cut(s) 713
Van91I CCANNNNNTGG 1 cut(s) 319
VpaK11BI GGWCC 1 cut(s) 21
XapI RAATTY 3 cut(s) 412, 802, 938
XmnI GAANNNNTTC 1 cut(s) 245
XspI CTAG 2 cut(s) 762, 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.