pycom12661g00140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012661
OG0001044
Physical Location & Seq
Forward (+)
87203 .. 88295
1093 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12661g00140.5

Sequence Viewer

Length: 840 bp
ATGCCACTAATTCGTTTCCTCAAGAGAATGATATTTGCAGTGGGAAAGAATTTCTCTAAGAATGCATGTTTCATACTTTCCCAAGAATTTACAGTTCCAGGAGACAATTCAAACAACCAATCTTTGGCCTTGTCCATAAGTGAGAATGGAAAGGCCTTCATCTTCAAAATACTCCCATCAACGTTGATGGGTGTCATACATGAGCAAACTACCTCAAATTCCTTAAGATGTAATAAACTAGACTTAAGTTCAAATTCTTCTGTCTTACCTTCAGCTGCCGTAGGGTATTGTATGCATAAAGGTGCGGCATTGCCCATACCTGAGGCAGAGAGCTCCTTGATGGTCCGATTGTCCACTGCCATGGTTGGTACTTCTTCTTTAAATTCAGGTTCGGGACTAGGAGGATTAGATGCTTGTGATTTCTTTTTCCTTCTCAAAGTCCTCTCGAAATCACCGTCAAAGTCGGAGATATGCTCACGAACAGTAAACAAGATAAGTAATCAACTTAATAGACTTGGCAAAAAAAAACAAAACACATACACACGGTGCAAAAAAAAAAAAATTAAAACAAAACAGAAACTAAACAATTTAAACAAGACTCAAGATAAGGGATTAGCAAATTTACGCACACAAATTAAACCCTCTTTTCGTCAAATTGTAGAGGGATTGCAAATCACTTGGAAACTGACTCAAAAACACTCAAACATCACCCTAAACACTCAAAACTGCCTTAAAAACACTTTCTGGGCAGTTTTGAGCACGTTGGTGAATATGGACGAATTTGGGAAACAAAATGAATCAAAACTCTTAAAAACACAAACTAAATGTAATTCTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

280

Amino Acids

30.88

Weight (kDa)

10.01

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 124
AciI CCGC 1 cut(s) 305
AclI AACGTT 1 cut(s) 182
AcsI RAATTY 7 cut(s) 49, 86, 217, 253, 382, 619, 779
AcuI CTGAAG 1 cut(s) 255
AdeI CACNNNGTG 1 cut(s) 546
AfaI GTAC 1 cut(s) 370
AfiI CCNNNNNNNGG 1 cut(s) 124
AflII CTTAAG 2 cut(s) 223, 244
AgsI TTSAA 3 cut(s) 111, 166, 252
AjnI CCWGG 1 cut(s) 97
AleI CACNNNNGTG 1 cut(s) 764
AluBI AGCT 2 cut(s) 275, 333
AluI AGCT 2 cut(s) 275, 333
Alw21I GWGCWC 2 cut(s) 335, 761
Alw26I GTCTC 1 cut(s) 96
AoxI GGCC 2 cut(s) 126, 153
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 7 cut(s) 49, 86, 217, 253, 382, 619, 779
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
AspS9I GGNCC 1 cut(s) 343
AsuHPI GGTGA 3 cut(s) 444, 700, 778
AvaII GGWCC 1 cut(s) 343
AxyI CCTNAGG 1 cut(s) 321
BanII GRGCYC 1 cut(s) 335
Bbv12I GWGCWC 2 cut(s) 335, 761
BbvI GCAGC 1 cut(s) 262
BccI CCATC 3 cut(s) 181, 184, 334
BceAI ACGGC 1 cut(s) 263
BciT130I CCWGG 1 cut(s) 99
BcoDI GTCTC 1 cut(s) 96
BfaI CTAG 2 cut(s) 239, 398
BfrI CTTAAG 2 cut(s) 223, 244
BisI GCNGC 2 cut(s) 276, 306
BlsI GCNGC 2 cut(s) 277, 307
Bme1390I CCNGG 1 cut(s) 99
Bme18I GGWCC 1 cut(s) 343
BmgT120I GGNCC 1 cut(s) 343
BmrFI CCNGG 1 cut(s) 99
BmsI GCATC 1 cut(s) 400
BplI GAGNNNNNCTC 2 cut(s) 458, 490
BpuEI CTTGAG 2 cut(s) 5, 585
BsaJI CCNNGG 1 cut(s) 360
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse21I CCTNAGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 308
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 360
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 1 cut(s) 312
BseXI GCAGC 1 cut(s) 262
BshFI GGCC 2 cut(s) 128, 155
BsiHKAI GWGCWC 2 cut(s) 335, 761
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 96
BsmFI GGGAC 1 cut(s) 408
BsmI GAATGC 1 cut(s) 67
BsnI GGCC 2 cut(s) 128, 155
Bsp1286I GDGCHC 2 cut(s) 335, 761
Bsp19I CCATGG 1 cut(s) 360
BspACI CCGC 1 cut(s) 305
BspANI GGCC 2 cut(s) 128, 155
BspCNI CTCAG 1 cut(s) 313
BspTI CTTAAG 2 cut(s) 223, 244
BsrDI GCAATG 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 360
BssT1I CCWWGG 1 cut(s) 360
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 4 cut(s) 94, 456, 484, 546
BstAFI CTTAAG 2 cut(s) 223, 244
BstDEI CTNAG 2 cut(s) 57, 321
BstDSI CCRYGG 1 cut(s) 360
BstMAI GTCTC 1 cut(s) 96
BstNI CCWGG 1 cut(s) 99
BstNSI RCATGY 1 cut(s) 69
BstSCI CCNGG 1 cut(s) 97
BstV1I GCAGC 1 cut(s) 262
BstXI CCANNNNNNTGG 1 cut(s) 361
Bsu36I CCTNAGG 1 cut(s) 321
BsuRI GGCC 2 cut(s) 128, 155
BtgI CCRYGG 1 cut(s) 360
BtsI GCAGTG 2 cut(s) 45, 354
BtsIMutI CAGTG 2 cut(s) 45, 354
Cfr13I GGNCC 1 cut(s) 343
Csp6I GTAC 1 cut(s) 369
CviAII CATG 3 cut(s) 66, 200, 361
CviJI RGCY 4 cut(s) 128, 155, 275, 333
CviKI_1 RGCY 4 cut(s) 128, 155, 275, 333
CviQI GTAC 1 cut(s) 369
DdeI CTNAG 2 cut(s) 57, 321
DraI TTTAAA 2 cut(s) 381, 591
DraIII CACNNNGTG 1 cut(s) 546
Ecl136II GAGCTC 1 cut(s) 333
Eco130I CCWWGG 1 cut(s) 360
Eco147I AGGCCT 1 cut(s) 155
Eco24I GRGCYC 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 343
Eco53kI GAGCTC 1 cut(s) 333
Eco57I CTGAAG 1 cut(s) 255
Eco81I CCTNAGG 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 360
EcoT22I ATGCAT 2 cut(s) 67, 297
EcoT38I GRGCYC 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 360
FaeI CATG 3 cut(s) 69, 203, 364
FaqI GGGAC 1 cut(s) 408
FatI CATG 3 cut(s) 65, 199, 360
Fnu4HI GCNGC 2 cut(s) 276, 306
FriOI GRGCYC 1 cut(s) 335
Fsp4HI GCNGC 2 cut(s) 276, 306
FspBI CTAG 2 cut(s) 239, 398
GluI GCNGC 2 cut(s) 276, 306
HaeIII GGCC 2 cut(s) 128, 155
Hin1II CATG 3 cut(s) 69, 203, 364
HinfI GANTC 3 cut(s) 598, 688, 797
HphI GGTGA 3 cut(s) 444, 700, 778
Hpy166II GTNNAC 2 cut(s) 354, 487
Hpy188I TCNGA 2 cut(s) 347, 466
Hpy188III TCNNGA 5 cut(s) 22, 393, 445, 477, 602
Hpy8I GTNNAC 2 cut(s) 354, 487
HpyAV CCTTC 3 cut(s) 166, 279, 440
HpyCH4III ACNGT 4 cut(s) 94, 456, 484, 546
HpyCH4IV ACGT 2 cut(s) 182, 761
HpyCH4V TGCA 5 cut(s) 38, 65, 295, 549, 670
HpyF3I CTNAG 2 cut(s) 57, 321
HpySE526I ACGT 2 cut(s) 182, 761
Hsp92II CATG 3 cut(s) 69, 203, 364
LmnI GCTCC 1 cut(s) 338
LpnPI CCDG 5 cut(s) 84, 111, 333, 372, 730
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 1 cut(s) 400
MaeI CTAG 2 cut(s) 239, 398
MaeII ACGT 2 cut(s) 182, 761
MboII GAAGA 3 cut(s) 154, 249, 366
MhlI GDGCHC 2 cut(s) 335, 761
MlyI GAGTC 2 cut(s) 592, 682
MmeI TCCRAC 1 cut(s) 444
MnlI CCTC 7 cut(s) 29, 223, 316, 395, 452, 652, 655
Mph1103I ATGCAT 2 cut(s) 67, 297
MseI TTAA 9 cut(s) 224, 245, 380, 507, 564, 590, 636, 732, 809
MslI CAYNNNNRTG 3 cut(s) 300, 359, 764
MspA1I CMGCKG 1 cut(s) 275
MspCI CTTAAG 2 cut(s) 223, 244
MspR9I CCNGG 1 cut(s) 99
Mva1269I GAATGC 1 cut(s) 67
MvaI CCWGG 1 cut(s) 99
NcoI CCATGG 1 cut(s) 360
NlaIII CATG 3 cut(s) 69, 203, 364
NsiI ATGCAT 2 cut(s) 67, 297
NspI RCATGY 1 cut(s) 69
OliI CACNNNNGTG 1 cut(s) 764
PceI AGGCCT 1 cut(s) 155
PcsI WCGNNNNNNNCGW 1 cut(s) 452
PctI GAATGC 1 cut(s) 67
PfeI GAWTC 1 cut(s) 797
PflMI CCANNNNNTGG 1 cut(s) 124
PfoI TCCNGGA 1 cut(s) 97
PkrI GCNGC 2 cut(s) 277, 307
PleI GAGTC 2 cut(s) 592, 682
PpsI GAGTC 2 cut(s) 592, 682
Psp124BI GAGCTC 1 cut(s) 335
Psp1406I AACGTT 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 97
PspPI GGNCC 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 1 cut(s) 370
RsaNI GTAC 1 cut(s) 369
RseI CAYNNNNRTG 3 cut(s) 300, 359, 764
SacI GAGCTC 1 cut(s) 335
SaqAI TTAA 9 cut(s) 224, 245, 380, 507, 564, 590, 636, 732, 809
SatI GCNGC 2 cut(s) 276, 306
Sau96I GGNCC 1 cut(s) 343
SchI GAGTC 2 cut(s) 592, 682
ScrFI CCNGG 1 cut(s) 99
SduI GDGCHC 2 cut(s) 335, 761
SetI ASST 9 cut(s) 185, 215, 271, 277, 304, 322, 335, 391, 764
SfaNI GCATC 1 cut(s) 400
SinI GGWCC 1 cut(s) 343
SmiMI CAYNNNNRTG 3 cut(s) 300, 359, 764
SmlI CTYRAG 4 cut(s) 20, 223, 244, 600
SmoI CTYRAG 4 cut(s) 20, 223, 244, 600
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 1 cut(s) 305
SspMI CTAG 2 cut(s) 239, 398
SstI GAGCTC 1 cut(s) 335
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 97
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 4 cut(s) 94, 456, 484, 546
TaiI ACGT 2 cut(s) 185, 764
TaqI TCGA 1 cut(s) 446
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 1 cut(s) 797
Tru1I TTAA 9 cut(s) 224, 245, 380, 507, 564, 590, 636, 732, 809
Tru9I TTAA 9 cut(s) 224, 245, 380, 507, 564, 590, 636, 732, 809
TscAI CASTG 2 cut(s) 45, 361
TseI GCWGC 1 cut(s) 275
TspDTI ATGAA 3 cut(s) 61, 148, 810
TspRI CASTG 2 cut(s) 45, 361
Van91I CCANNNNNTGG 1 cut(s) 124
Vha464I CTTAAG 2 cut(s) 223, 244
VpaK11BI GGWCC 1 cut(s) 343
XapI RAATTY 7 cut(s) 49, 86, 217, 253, 382, 619, 779
XceI RCATGY 1 cut(s) 69
XspI CTAG 2 cut(s) 239, 398
Zsp2I ATGCAT 2 cut(s) 67, 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.