pycom436g00480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_436
OG0001044
Physical Location & Seq
Forward (+)
548578 .. 549807
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom436g00480.1

Sequence Viewer

Length: 912 bp
ATGCTATCCATTCCAAGCCCAATATTGCTCCAAAATGCACTTTCTTTCCACTTTAACCATTTGGACCTACAACCATACGAAAATAGAATTAAACTAACAAAGTCGCATAAATATGCTCCTATCAAGGTTATTGGCTGCCATGGGAGTTTTATCCACCAACGCACCTCCCGCCGAGGCATCAAGCATTTGCCTTTCAATGGGAAGAAGACCTTCATAGAAGATGCAACAAGGGATTTAAAACGCTCATACTTCTTTCTCAAGAGAATGACTCTTGAAGTAGGAAAGAACTTCTCAAGAAACGCCCTCTTCATGCTCTCCCAAGAAGTGACCGTTCCGGGTGCTAACTTGTATAACCAATCCTTGGCCTTCTCCAATAATGAGAACGGGAAAGCCTTCATCTTCAAAATACTACTATCAACGTTGATTGGAGTCATACTTGAACACACCACCTCGAATTCCTTCAAATGCTTGTTGGGATCCTCCATGGACAGTCCATGGAACTTCGGAATGTGGTCCGCCCTTGGGTATTGGATGCATAAGGGTGCTGCATTGTCCATTCCCGAAGCGGAAAGCTCCTTGATTGTACGGTTGTCCACTGCCATGGTTTGGACCTCTTCTTGCTCCCTTGCCGTGGGGGTGAAGTTCTGGGTTCTTCTTCTTCTTTCTCAAAGTGCGCTCAAAATCGTCGTCAAAATCCAAGATGTTTGCACTAATAGGTTGAGAACTACGAATCATAAACTAGTACCTACAAACATGAAAAGAAATAAGAAACAACGGGGGATTAGCAAAGTTGCTAATCCCCGGCAACGGTGCCAAAAATTTGATGTGATATATAATACACAAAGATCGATCGTTCTAGGCCGGGGATTAGGAGGGATTGCTAATCTAATGAAAATCGACTCAAAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

304

Amino Acids

34.23

Weight (kDa)

10.32

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 810
AccB7I CCANNNNNTGG 2 cut(s) 361, 606
AciI CCGC 3 cut(s) 169, 516, 566
AclI AACGTT 1 cut(s) 419
AclWI GGATC 2 cut(s) 471, 484
AcsI RAATTY 2 cut(s) 454, 818
AfaI GTAC 2 cut(s) 585, 744
AfiI CCNNNNNNNGG 6 cut(s) 197, 361, 522, 606, 631, 807
AgsI TTSAA 5 cut(s) 196, 275, 403, 440, 463
AhlI ACTAGT 1 cut(s) 739
AluBI AGCT 1 cut(s) 573
AluI AGCT 1 cut(s) 573
AlwI GGATC 2 cut(s) 471, 484
AoxI GGCC 2 cut(s) 363, 859
ApeKI GCWGC 2 cut(s) 135, 545
ApoI RAATTY 2 cut(s) 454, 818
ArsI GACNNNNNNTTYG 2 cut(s) 672, 704
Asp700I GAANNNNTTC 3 cut(s) 209, 392, 458
AspLEI GCGC 1 cut(s) 676
AspS9I GGNCC 3 cut(s) 64, 513, 609
AsuC2I CCSGG 3 cut(s) 336, 802, 863
AsuHPI GGTGA 1 cut(s) 649
AvaII GGWCC 3 cut(s) 64, 513, 609
BamHI GGATCC 1 cut(s) 476
BanI GGYRCC 1 cut(s) 810
BbsI GAAGAC 1 cut(s) 212
BbvI GCAGC 2 cut(s) 122, 532
BceAI ACGGC 1 cut(s) 614
BcnI CCSGG 3 cut(s) 336, 802, 863
BcuI ACTAGT 1 cut(s) 739
BfaI CTAG 2 cut(s) 740, 857
BisI GCNGC 2 cut(s) 136, 546
BlsI GCNGC 2 cut(s) 137, 547
Bme1390I CCNGG 3 cut(s) 336, 802, 863
Bme18I GGWCC 3 cut(s) 64, 513, 609
BmgT120I GGNCC 3 cut(s) 64, 513, 609
BmiI GGNNCC 2 cut(s) 478, 812
BmrFI CCNGG 3 cut(s) 336, 802, 863
BmsI GCATC 3 cut(s) 186, 211, 522
BpiI GAAGAC 1 cut(s) 212
BplI GAGNNNNNCTC 2 cut(s) 253, 285
BpuEI CTTGAG 2 cut(s) 242, 277
BpuMI CCSGG 3 cut(s) 336, 802, 863
Bsa29I ATCGAT 1 cut(s) 848
Bsc4I CCNNNNNNNGG 6 cut(s) 197, 361, 522, 606, 631, 807
BseCI ATCGAT 1 cut(s) 848
BseGI GGATG 1 cut(s) 537
BseLI CCNNNNNNNGG 6 cut(s) 197, 361, 522, 606, 631, 807
BseXI GCAGC 2 cut(s) 122, 532
Bsh1285I CGRYCG 1 cut(s) 852
BshFI GGCC 2 cut(s) 365, 861
BshNI GGYRCC 1 cut(s) 810
BshVI ATCGAT 1 cut(s) 848
BsiEI CGRYCG 1 cut(s) 852
BsiSI CCGG 3 cut(s) 335, 802, 862
BslI CCNNNNNNNGG 6 cut(s) 197, 361, 522, 606, 631, 807
BsnI GGCC 2 cut(s) 365, 861
Bsp143I GATC 3 cut(s) 476, 845, 849
Bsp19I CCATGG 4 cut(s) 139, 483, 494, 600
BspACI CCGC 3 cut(s) 169, 516, 566
BspANI GGCC 2 cut(s) 365, 861
BspDI ATCGAT 1 cut(s) 848
BspLI GGNNCC 2 cut(s) 478, 812
BspPI GGATC 2 cut(s) 471, 484
BspT107I GGYRCC 1 cut(s) 810
BssMI GATC 3 cut(s) 476, 845, 849
BssT1I CCWWGG 6 cut(s) 139, 360, 483, 494, 520, 600
Bst4CI ACNGT 4 cut(s) 331, 491, 588, 810
Bst6I CTCTTC 2 cut(s) 311, 619
BstDSI CCRYGG 5 cut(s) 139, 483, 494, 600, 630
BstF5I GGATG 1 cut(s) 537
BstHHI GCGC 1 cut(s) 676
BstKTI GATC 3 cut(s) 479, 848, 852
BstMBI GATC 3 cut(s) 476, 845, 849
BstMCI CGRYCG 1 cut(s) 852
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstSCI CCNGG 3 cut(s) 334, 800, 861
BstV1I GCAGC 2 cut(s) 122, 532
BstV2I GAAGAC 1 cut(s) 212
BstX2I RGATCY 1 cut(s) 476
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 1 cut(s) 476
Bsu15I ATCGAT 1 cut(s) 848
BsuRI GGCC 2 cut(s) 365, 861
BsuTUI ATCGAT 1 cut(s) 848
BtgI CCRYGG 5 cut(s) 139, 483, 494, 600, 630
BtsCI GGATG 1 cut(s) 537
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 1 cut(s) 594
CfoI GCGC 1 cut(s) 676
Cfr13I GGNCC 3 cut(s) 64, 513, 609
ClaI ATCGAT 1 cut(s) 848
Csp6I GTAC 2 cut(s) 584, 743
CviAII CATG 6 cut(s) 140, 310, 484, 495, 601, 754
CviJI RGCY 6 cut(s) 18, 135, 365, 392, 573, 861
CviKI_1 RGCY 6 cut(s) 18, 135, 365, 392, 573, 861
CviQI GTAC 2 cut(s) 584, 743
DpnI GATC 3 cut(s) 478, 847, 851
DpnII GATC 3 cut(s) 476, 845, 849
DraI TTTAAA 1 cut(s) 237
Eam1104I CTCTTC 2 cut(s) 311, 619
EarI CTCTTC 2 cut(s) 311, 619
EciI GGCGGA 1 cut(s) 505
Eco130I CCWWGG 6 cut(s) 139, 360, 483, 494, 520, 600
Eco47I GGWCC 3 cut(s) 64, 513, 609
EcoRI GAATTC 1 cut(s) 454
EcoT14I CCWWGG 6 cut(s) 139, 360, 483, 494, 520, 600
EcoT22I ATGCAT 1 cut(s) 537
ErhI CCWWGG 6 cut(s) 139, 360, 483, 494, 520, 600
FaeI CATG 6 cut(s) 143, 313, 487, 498, 604, 757
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FatI CATG 6 cut(s) 139, 309, 483, 494, 600, 753
FauI CCCGC 1 cut(s) 176
Fnu4HI GCNGC 2 cut(s) 136, 546
FokI GGATG 1 cut(s) 544
Fsp4HI GCNGC 2 cut(s) 136, 546
FspBI CTAG 2 cut(s) 740, 857
GlaI GCGC 1 cut(s) 675
GluI GCNGC 2 cut(s) 136, 546
HaeIII GGCC 2 cut(s) 365, 861
HapII CCGG 3 cut(s) 335, 802, 862
HhaI GCGC 1 cut(s) 676
Hin1II CATG 6 cut(s) 143, 313, 487, 498, 604, 757
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HinfI GANTC 4 cut(s) 268, 429, 730, 899
HpaII CCGG 3 cut(s) 335, 802, 862
HphI GGTGA 1 cut(s) 649
Hpy166II GTNNAC 1 cut(s) 594
Hpy188I TCNGA 1 cut(s) 506
Hpy188III TCNNGA 4 cut(s) 259, 272, 294, 560
Hpy8I GTNNAC 1 cut(s) 594
Hpy99I CGWCG 1 cut(s) 689
HpyAV CCTTC 4 cut(s) 220, 376, 403, 469
HpyCH4III ACNGT 4 cut(s) 331, 491, 588, 810
HpyCH4IV ACGT 1 cut(s) 419
HpyCH4V TGCA 5 cut(s) 38, 224, 535, 548, 708
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpySE526I ACGT 1 cut(s) 419
Hsp92II CATG 6 cut(s) 143, 313, 487, 498, 604, 757
HspAI GCGC 1 cut(s) 674
Kzo9I GATC 3 cut(s) 476, 845, 849
LmnI GCTCC 4 cut(s) 33, 121, 578, 626
LpnPI CCDG 4 cut(s) 348, 631, 815, 875
Lsp1109I GCAGC 2 cut(s) 122, 532
LweI GCATC 3 cut(s) 186, 211, 522
MaeI CTAG 2 cut(s) 740, 857
MaeII ACGT 1 cut(s) 419
MaeIII GTNAC 1 cut(s) 325
MalI GATC 3 cut(s) 478, 847, 851
MboI GATC 3 cut(s) 476, 845, 849
MboII GAAGA 9 cut(s) 214, 217, 230, 298, 391, 606, 644, 647, 650
MflI RGATCY 1 cut(s) 476
MluCI AATT 3 cut(s) 87, 454, 818
MlyI GAGTC 3 cut(s) 262, 438, 893
MnlI CCTC 7 cut(s) 167, 175, 314, 460, 490, 622, 866
Mph1103I ATGCAT 1 cut(s) 537
MroXI GAANNNNTTC 3 cut(s) 209, 392, 458
MseI TTAA 3 cut(s) 54, 90, 236
MslI CAYNNNNRTG 3 cut(s) 111, 540, 599
MspI CCGG 3 cut(s) 335, 802, 862
MspR9I CCNGG 3 cut(s) 336, 802, 863
MwoI GCNNNNNNNGC 1 cut(s) 168
NciI CCSGG 3 cut(s) 336, 802, 863
NcoI CCATGG 4 cut(s) 139, 483, 494, 600
NdeII GATC 3 cut(s) 476, 845, 849
NlaIII CATG 6 cut(s) 143, 313, 487, 498, 604, 757
NlaIV GGNNCC 2 cut(s) 478, 812
NmeAIII GCCGAG 1 cut(s) 197
NmuCI GTSAC 1 cut(s) 325
NsiI ATGCAT 1 cut(s) 537
PdmI GAANNNNTTC 3 cut(s) 209, 392, 458
PfeI GAWTC 1 cut(s) 730
PflMI CCANNNNNTGG 2 cut(s) 361, 606
PkrI GCNGC 2 cut(s) 137, 547
Ple19I CGATCG 1 cut(s) 852
PleI GAGTC 3 cut(s) 262, 437, 893
PpsI GAGTC 3 cut(s) 262, 437, 893
Psp1406I AACGTT 1 cut(s) 419
PspN4I GGNNCC 2 cut(s) 478, 812
PspPI GGNCC 3 cut(s) 64, 513, 609
PsuI RGATCY 1 cut(s) 476
PvuI CGATCG 1 cut(s) 852
RsaI GTAC 2 cut(s) 585, 744
RsaNI GTAC 2 cut(s) 584, 743
RseI CAYNNNNRTG 3 cut(s) 111, 540, 599
SaqAI TTAA 3 cut(s) 54, 90, 236
SatI GCNGC 2 cut(s) 136, 546
Sau3AI GATC 3 cut(s) 476, 845, 849
Sau96I GGNCC 3 cut(s) 64, 513, 609
SchI GAGTC 3 cut(s) 262, 438, 893
ScrFI CCNGG 3 cut(s) 336, 802, 863
SfaNI GCATC 3 cut(s) 186, 211, 522
SinI GGWCC 3 cut(s) 64, 513, 609
SmiMI CAYNNNNRTG 3 cut(s) 111, 540, 599
SmlI CTYRAG 2 cut(s) 257, 292
SmoI CTYRAG 2 cut(s) 257, 292
SpeI ACTAGT 1 cut(s) 739
Sse9I AATT 3 cut(s) 87, 454, 818
SsiI CCGC 3 cut(s) 169, 516, 566
SspI AATATT 1 cut(s) 24
SspMI CTAG 2 cut(s) 740, 857
StyD4I CCNGG 3 cut(s) 334, 800, 861
StyI CCWWGG 6 cut(s) 139, 360, 483, 494, 520, 600
TaaI ACNGT 4 cut(s) 331, 491, 588, 810
TaiI ACGT 1 cut(s) 422
TaqI TCGA 3 cut(s) 452, 848, 897
TasI AATT 3 cut(s) 87, 454, 818
TfiI GAWTC 1 cut(s) 730
Tru1I TTAA 3 cut(s) 54, 90, 236
Tru9I TTAA 3 cut(s) 54, 90, 236
TscAI CASTG 1 cut(s) 601
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 2 cut(s) 135, 545
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 5 cut(s) 202, 298, 385, 770, 905
TspRI CASTG 1 cut(s) 601
Van91I CCANNNNNTGG 2 cut(s) 361, 606
VpaK11BI GGWCC 3 cut(s) 64, 513, 609
XapI RAATTY 2 cut(s) 454, 818
XmnI GAANNNNTTC 3 cut(s) 209, 392, 458
XspI CTAG 2 cut(s) 740, 857
Zsp2I ATGCAT 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.