pycom12426g00380

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012426
OG0001044
Physical Location & Seq
Forward (+)
214078 .. 214371
294 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12426g00380.1

Sequence Viewer

Length: 294 bp
ATGACTCTTGAAGTAGGAAAGAACTTTTCAAGAAACGCCCTCTTCATGCTCTCCCAAGAGGTGACAGTTCCGGGTGCTAACTCGTATAACCAATCCTTGGCCTTCTCCAAACGTGAGAACGGGAAAGCCTTCATCTTCAAAATGCTACCATCAACGTTAACTGGAGTCATGCTCGAGCACACCACCTCGAACTCCTTCAAATGCTTGTTGGGATCCTCCATGGACAGTCCATGGAACTTTGGAATGTGGTGCAACAAGCTTGACTTCAATTCAAATTCGTCGGTCTTGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

98

Amino Acids

10.77

Weight (kDa)

8.71

Isoelectric Point (pI)

32.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 97
AclI AACGTT 1 cut(s) 155
AclWI GGATC 2 cut(s) 207, 220
AcsI RAATTY 1 cut(s) 274
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 6 cut(s) 11, 30, 139, 199, 268, 273
AluBI AGCT 1 cut(s) 259
AluI AGCT 1 cut(s) 259
Alw21I GWGCWC 1 cut(s) 180
AlwI GGATC 2 cut(s) 207, 220
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 1 cut(s) 99
ApoI RAATTY 1 cut(s) 274
Asp700I GAANNNNTTC 2 cut(s) 128, 194
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 173
BamHI GGATCC 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 180
BccI CCATC 1 cut(s) 157
BcnI CCSGG 1 cut(s) 72
Bme1390I CCNGG 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 173
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 1 cut(s) 72
BplI GAGNNNNNCTC 2 cut(s) 156, 188
BpmI CTGGAG 1 cut(s) 183
BpuMI CCSGG 1 cut(s) 72
BsaJI CCNNGG 3 cut(s) 96, 219, 230
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse1I ACTGG 1 cut(s) 166
BseDI CCNNGG 3 cut(s) 96, 219, 230
BseLI CCNNNNNNNGG 1 cut(s) 97
BseNI ACTGG 1 cut(s) 166
BshFI GGCC 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 180
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 1 cut(s) 71
BslI CCNNNNNNNGG 1 cut(s) 97
BsnI GGCC 1 cut(s) 101
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 1 cut(s) 212
Bsp19I CCATGG 2 cut(s) 219, 230
BspANI GGCC 1 cut(s) 101
BspLI GGNNCC 1 cut(s) 214
BspPI GGATC 2 cut(s) 207, 220
BsrI ACTGG 1 cut(s) 166
BssECI CCNNGG 3 cut(s) 96, 219, 230
BssMI GATC 1 cut(s) 212
BssT1I CCWWGG 3 cut(s) 96, 219, 230
Bst4CI ACNGT 2 cut(s) 67, 227
Bst6I CTCTTC 1 cut(s) 47
BstDSI CCRYGG 2 cut(s) 219, 230
BstKTI GATC 1 cut(s) 215
BstMBI GATC 1 cut(s) 212
BstSCI CCNGG 1 cut(s) 70
BstX2I RGATCY 1 cut(s) 212
BstYI RGATCY 1 cut(s) 212
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 2 cut(s) 219, 230
CviAII CATG 4 cut(s) 46, 169, 220, 231
CviJI RGCY 3 cut(s) 101, 128, 259
CviKI_1 RGCY 3 cut(s) 101, 128, 259
DpnI GATC 1 cut(s) 214
DpnII GATC 1 cut(s) 212
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Eco130I CCWWGG 3 cut(s) 96, 219, 230
Eco88I CYCGRG 1 cut(s) 173
EcoT14I CCWWGG 3 cut(s) 96, 219, 230
ErhI CCWWGG 3 cut(s) 96, 219, 230
FaeI CATG 4 cut(s) 49, 172, 223, 234
FaiI YATR 5 cut(s) 47, 87, 170, 221, 232
FalI AAGNNNNNCTT 2 cut(s) 248, 280
FatI CATG 4 cut(s) 45, 168, 219, 230
GsuI CTGGAG 1 cut(s) 183
HaeIII GGCC 1 cut(s) 101
HapII CCGG 1 cut(s) 71
Hin1II CATG 4 cut(s) 49, 172, 223, 234
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 2 cut(s) 4, 165
HpaI GTTAAC 1 cut(s) 159
HpaII CCGG 1 cut(s) 71
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 159
Hpy188III TCNNGA 2 cut(s) 8, 30
Hpy8I GTNNAC 1 cut(s) 159
Hpy99I CGWCG 1 cut(s) 283
HpyAV CCTTC 3 cut(s) 112, 139, 205
HpyCH4III ACNGT 2 cut(s) 67, 227
HpyCH4IV ACGT 2 cut(s) 112, 155
HpyCH4V TGCA 1 cut(s) 252
HpySE526I ACGT 2 cut(s) 112, 155
Hsp92II CATG 4 cut(s) 49, 172, 223, 234
KspAI GTTAAC 1 cut(s) 159
Kzo9I GATC 1 cut(s) 212
LpnPI CCDG 2 cut(s) 84, 147
MaeII ACGT 2 cut(s) 112, 155
MaeIII GTNAC 1 cut(s) 61
MalI GATC 1 cut(s) 214
MboI GATC 1 cut(s) 212
MboII GAAGA 2 cut(s) 34, 127
MflI RGATCY 1 cut(s) 212
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 2 cut(s) 268, 274
MlyI GAGTC 1 cut(s) 174
MnlI CCTC 4 cut(s) 50, 52, 196, 226
MroXI GAANNNNTTC 2 cut(s) 128, 194
MseI TTAA 2 cut(s) 158, 292
MspI CCGG 1 cut(s) 71
MspR9I CCNGG 1 cut(s) 72
NciI CCSGG 1 cut(s) 72
NcoI CCATGG 2 cut(s) 219, 230
NdeII GATC 1 cut(s) 212
NlaIII CATG 4 cut(s) 49, 172, 223, 234
NlaIV GGNNCC 1 cut(s) 214
NmuCI GTSAC 1 cut(s) 61
PaeR7I CTCGAG 1 cut(s) 173
PdmI GAANNNNTTC 2 cut(s) 128, 194
PflMI CCANNNNNTGG 1 cut(s) 97
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
Psp1406I AACGTT 1 cut(s) 155
PspN4I GGNNCC 1 cut(s) 214
PspXI VCTCGAGB 1 cut(s) 173
PsuI RGATCY 1 cut(s) 212
SaqAI TTAA 2 cut(s) 158, 292
Sau3AI GATC 1 cut(s) 212
SchI GAGTC 1 cut(s) 174
ScrFI CCNGG 1 cut(s) 72
SduI GDGCHC 1 cut(s) 180
SetI ASST 5 cut(s) 63, 115, 158, 188, 261
Sfr274I CTCGAG 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 173
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 2 cut(s) 268, 274
StyD4I CCNGG 1 cut(s) 70
StyI CCWWGG 3 cut(s) 96, 219, 230
TaaI ACNGT 2 cut(s) 67, 227
TaiI ACGT 2 cut(s) 115, 158
TaqI TCGA 2 cut(s) 174, 188
TaqII GACCGA 1 cut(s) 271
TasI AATT 2 cut(s) 268, 274
Tru1I TTAA 2 cut(s) 158, 292
Tru9I TTAA 2 cut(s) 158, 292
TseFI GTSAC 1 cut(s) 61
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 2 cut(s) 34, 121
Van91I CCANNNNNTGG 1 cut(s) 97
XapI RAATTY 1 cut(s) 274
XhoI CTCGAG 1 cut(s) 173
XmnI GAANNNNTTC 2 cut(s) 128, 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.