pycom13g25420

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001044
Physical Location & Seq
Forward (+)
22229445 .. 22230062
618 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g25420.1

Sequence Viewer

Length: 510 bp
ATGCTCGAACAAACCACCTCGAACTCCTTCAAGTGTTTGTTGGGGTCTTCCATAGACAAGCCATGGAACTTCGAAATATGGTGTAACAAACTGGATTTGAGTTCAAATTCCTCAGTCTTCCCCTCTACAGCCGTGGGGTATTGGATACAAAGAGGCAATGCATTATCCAAACCCGAGGCCGAAAGCTCCTTCATGGTTCTATTATCTGCTGCCATGCCCAGGTTGTGGTGGAGGTTGTGGTGGCTCTTGTTGACTCCTCGTTCTCCTCAAAGTGCGTTCAAAATCACCGTCAAAGTCAGAGATGTTCTCTATAATAGGCCGAGAGCGACGAAATCTGAAATAAGAATGCACGAAAAAAAACTAAAAAAATATAAAAATAAAGACAAAGGATTAGCAAAGTTGCTAATCCCCGGCAACGGCGCCAAAATTTGGTACGAGATTTACTTGACACTCAAATTAAACCCTCGTTTGACAATTGTAGTAGAATGGATAAGTGAGGGATCGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

19.58

Weight (kDa)

9.93

Isoelectric Point (pI)

31.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 419
AccB7I CCANNNNNTGG 2 cut(s) 225, 429
AclWI GGATC 1 cut(s) 508
AcsI RAATTY 2 cut(s) 106, 426
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 4 cut(s) 219, 225, 416, 429
AgsI TTSAA 3 cut(s) 31, 105, 280
AjnI CCWGG 1 cut(s) 218
AluBI AGCT 1 cut(s) 186
AluI AGCT 1 cut(s) 186
AlwI GGATC 1 cut(s) 508
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 2 cut(s) 177, 317
ApeKI GCWGC 1 cut(s) 209
ApoI RAATTY 2 cut(s) 106, 426
ArsI GACNNNNNNTTYG 2 cut(s) 273, 305
Asp700I GAANNNNTTC 1 cut(s) 26
AspLEI GCGC 1 cut(s) 422
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 1 cut(s) 277
AsuII TTCGAA 1 cut(s) 72
AvaI CYCGRG 1 cut(s) 173
BanI GGYRCC 1 cut(s) 419
BbsI GAAGAC 2 cut(s) 39, 109
BbvI GCAGC 1 cut(s) 196
BceAI ACGGC 2 cut(s) 116, 433
BciT130I CCWGG 1 cut(s) 220
BciVI GTATCC 1 cut(s) 138
BcnI CCSGG 1 cut(s) 411
BfaI CTAG 1 cut(s) 508
BfmI CTRYAG 1 cut(s) 126
BfoI RGCGCY 1 cut(s) 423
BfuI GTATCC 1 cut(s) 138
BisI GCNGC 1 cut(s) 210
BlsI GCNGC 1 cut(s) 211
Bme1390I CCNGG 2 cut(s) 220, 411
BmeT110I CYCGRG 1 cut(s) 173
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 2 cut(s) 220, 411
BpiI GAAGAC 2 cut(s) 39, 109
BplI GAGNNNNNCTC 2 cut(s) 291, 323
Bpu14I TTCGAA 1 cut(s) 72
BpuMI CCSGG 1 cut(s) 411
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 5 cut(s) 62, 132, 174, 218, 409
Bsc4I CCNNNNNNNGG 4 cut(s) 219, 225, 416, 429
Bse1I ACTGG 1 cut(s) 96
Bse3DI GCAATG 1 cut(s) 163
BseBI CCWGG 1 cut(s) 220
BseDI CCNNGG 5 cut(s) 62, 132, 174, 218, 409
BseLI CCNNNNNNNGG 4 cut(s) 219, 225, 416, 429
BseMI GCAATG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 1 cut(s) 96
BseRI GAGGAG 2 cut(s) 246, 255
BseXI GCAGC 1 cut(s) 196
BshFI GGCC 2 cut(s) 179, 319
BshNI GGYRCC 1 cut(s) 419
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 1 cut(s) 411
BslI CCNNNNNNNGG 4 cut(s) 219, 225, 416, 429
BsmI GAATGC 1 cut(s) 351
BsnI GGCC 2 cut(s) 179, 319
BsoBI CYCGRG 1 cut(s) 173
Bsp119I TTCGAA 1 cut(s) 72
Bsp143I GATC 1 cut(s) 500
Bsp19I CCATGG 1 cut(s) 62
BspANI GGCC 2 cut(s) 179, 319
BspCNI CTCAG 1 cut(s) 125
BspLI GGNNCC 1 cut(s) 421
BspPI GGATC 1 cut(s) 508
BspT104I TTCGAA 1 cut(s) 72
BspT107I GGYRCC 1 cut(s) 419
BsrDI GCAATG 1 cut(s) 163
BsrI ACTGG 1 cut(s) 96
BssECI CCNNGG 5 cut(s) 62, 132, 174, 218, 409
BssMI GATC 1 cut(s) 500
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 1 cut(s) 62
Bst2UI CCWGG 1 cut(s) 220
Bst4CI ACNGT 1 cut(s) 289
BstACI GRCGYC 1 cut(s) 420
BstBI TTCGAA 1 cut(s) 72
BstDEI CTNAG 1 cut(s) 112
BstDSI CCRYGG 2 cut(s) 62, 132
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 1 cut(s) 422
BstKTI GATC 1 cut(s) 503
BstMBI GATC 1 cut(s) 500
BstNI CCWGG 1 cut(s) 220
BstSCI CCNGG 2 cut(s) 218, 409
BstSFI CTRYAG 1 cut(s) 126
BstV1I GCAGC 1 cut(s) 196
BstV2I GAAGAC 2 cut(s) 39, 109
BsuI GTATCC 1 cut(s) 138
BsuRI GGCC 2 cut(s) 179, 319
BtgI CCRYGG 2 cut(s) 62, 132
CfoI GCGC 1 cut(s) 422
Csp6I GTAC 1 cut(s) 433
CviAII CATG 3 cut(s) 63, 193, 214
CviJI RGCY 6 cut(s) 61, 131, 179, 186, 244, 319
CviKI_1 RGCY 6 cut(s) 61, 131, 179, 186, 244, 319
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 1 cut(s) 112
DinI GGCGCC 1 cut(s) 421
DpnI GATC 1 cut(s) 502
DpnII GATC 1 cut(s) 500
Eco130I CCWWGG 1 cut(s) 62
Eco88I CYCGRG 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 218
EcoT14I CCWWGG 1 cut(s) 62
EcoT22I ATGCAT 1 cut(s) 163
EgeI GGCGCC 1 cut(s) 421
EheI GGCGCC 1 cut(s) 421
ErhI CCWWGG 1 cut(s) 62
FaeI CATG 3 cut(s) 66, 196, 217
FaiI YATR 7 cut(s) 53, 64, 79, 194, 215, 312, 372
FatI CATG 3 cut(s) 62, 192, 213
Fnu4HI GCNGC 1 cut(s) 210
Fsp4HI GCNGC 1 cut(s) 210
FspBI CTAG 1 cut(s) 508
GlaI GCGC 1 cut(s) 421
GluI GCNGC 1 cut(s) 210
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 2 cut(s) 179, 319
HapII CCGG 1 cut(s) 411
HhaI GCGC 1 cut(s) 422
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 3 cut(s) 66, 196, 217
Hin6I GCGC 1 cut(s) 420
HinP1I GCGC 1 cut(s) 420
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HinfI GANTC 1 cut(s) 253
HpaII CCGG 1 cut(s) 411
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 1 cut(s) 252
Hpy188I TCNGA 2 cut(s) 299, 337
Hpy8I GTNNAC 1 cut(s) 252
Hpy99I CGWCG 1 cut(s) 331
HpyAV CCTTC 2 cut(s) 37, 199
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4V TGCA 2 cut(s) 161, 349
HpyF3I CTNAG 1 cut(s) 112
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 3 cut(s) 66, 196, 217
HspAI GCGC 1 cut(s) 420
KasI GGCGCC 1 cut(s) 419
Kzo9I GATC 1 cut(s) 500
LmnI GCTCC 1 cut(s) 191
LpnPI CCDG 4 cut(s) 77, 205, 232, 424
Lsp1109I GCAGC 1 cut(s) 196
MaeI CTAG 1 cut(s) 508
MaeIII GTNAC 1 cut(s) 83
MalI GATC 1 cut(s) 502
MboI GATC 1 cut(s) 500
MboII GAAGA 2 cut(s) 39, 109
MfeI CAATTG 1 cut(s) 474
MluCI AATT 4 cut(s) 106, 426, 455, 474
Mly113I GGCGCC 1 cut(s) 420
MlyI GAGTC 1 cut(s) 247
Mph1103I ATGCAT 1 cut(s) 163
MroXI GAANNNNTTC 1 cut(s) 26
MseI TTAA 1 cut(s) 458
MspI CCGG 1 cut(s) 411
MspR9I CCNGG 2 cut(s) 220, 411
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 1 cut(s) 351
MvaI CCWGG 1 cut(s) 220
NarI GGCGCC 1 cut(s) 420
NciI CCSGG 1 cut(s) 411
NcoI CCATGG 1 cut(s) 62
NdeII GATC 1 cut(s) 500
NlaIII CATG 3 cut(s) 66, 196, 217
NlaIV GGNNCC 1 cut(s) 421
NmeAIII GCCGAG 1 cut(s) 345
NsiI ATGCAT 1 cut(s) 163
NspV TTCGAA 1 cut(s) 72
PctI GAATGC 1 cut(s) 351
PdmI GAANNNNTTC 1 cut(s) 26
PflMI CCANNNNNTGG 2 cut(s) 225, 429
PkrI GCNGC 1 cut(s) 211
PleI GAGTC 1 cut(s) 247
PluTI GGCGCC 1 cut(s) 423
PpsI GAGTC 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 218
PspGI CCWGG 1 cut(s) 218
PspN4I GGNNCC 1 cut(s) 421
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 1 cut(s) 210
Sau3AI GATC 1 cut(s) 500
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 2 cut(s) 220, 411
SetI ASST 4 cut(s) 20, 188, 224, 236
SfcI CTRYAG 1 cut(s) 126
SfoI GGCGCC 1 cut(s) 421
SfuI TTCGAA 1 cut(s) 72
Sse9I AATT 4 cut(s) 106, 426, 455, 474
SspDI GGCGCC 1 cut(s) 419
SspMI CTAG 1 cut(s) 508
StyD4I CCNGG 2 cut(s) 218, 409
StyI CCWWGG 1 cut(s) 62
TaaI ACNGT 1 cut(s) 289
TaqI TCGA 3 cut(s) 6, 20, 72
TasI AATT 4 cut(s) 106, 426, 455, 474
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TseI GCWGC 1 cut(s) 209
TspDTI ATGAA 1 cut(s) 181
Van91I CCANNNNNTGG 2 cut(s) 225, 429
XapI RAATTY 2 cut(s) 106, 426
XmnI GAANNNNTTC 1 cut(s) 26
XspI CTAG 1 cut(s) 508
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.