pycom12426g00220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012426
OG0001044
Physical Location & Seq
Forward (+)
126207 .. 127205
999 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12426g00220.1

Sequence Viewer

Length: 801 bp
ATGATCACACCAACCGGGATTGTAACCCACGGCATTGAGTGTCTCCCACCCTCCATTCTCGATCAATTGTGGGCACTTGTCCGTAGGGTGTCCTTGCATGGAGCATACGCCACAAGAAGCTACGGTTTTGACTTTTGGACCTTCCACCACCTGAGAAAGCAACGTAGTAAGGTTAGCCATTTGATTTTGAATTTCGGTTATGGCACTTACCTCATTGACTTGACGTTACCGTGGCATGCTCCTTTGTCCAACACCTTCGTATTGTTGAGCGTTCAACGCTCGATTAGCAATCAACATCTTTGCAGCCGTAGGGGTCTTGTCCACCAATTCCCTTGTAGAAGTATTGTAGAAGTAGTTCCTCTTTCATTTGATGTTGTGGACATGAATCCACTTATCCTTTTTCGTAGGAGGATGACTCGAGAAGTTGGGAAAAACTTCTCCAAGTATGCTCGTTTCATGCTCTCCCATGATGTGACAGTTCCGGGAGCCAATTCGTACAACCAATCTTTCGCTTTCTCTTGGAGAGAAAAGGGAAAGGCCTTCATCTTCAAGATGCTCTCATCCACTTTCACCAGAGTCATGCTTGAGCAAACGACTTCAAACTCCTTCAAGTGCTTGTTCGGATCTTCCATGGATATCGAACTCGGCAGTCTTGTCTTGAGCCGCCCTTGGGTATTGGATGCACAAGGGTGCGGCATTGGCCAATCCCAAAGCGGAAAGCTCCTTGATTGTTCGGTTGTCCGCTGCCATGTCTACCACTTCTTCTTCAAATTCAGAAACGGACTCGGAACTTGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

267

Amino Acids

30.33

Weight (kDa)

9.75

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 753
AciI CCGC 4 cut(s) 664, 693, 714, 742
AclWI GGATC 1 cut(s) 631
AcoI YGGCCR 1 cut(s) 700
AcsI RAATTY 2 cut(s) 190, 770
AfaI GTAC 1 cut(s) 497
AfiI CCNNNNNNNGG 1 cut(s) 670
AgsI TTSAA 6 cut(s) 190, 275, 550, 600, 610, 769
AleI CACNNNNGTG 1 cut(s) 688
AluBI AGCT 2 cut(s) 120, 721
AluI AGCT 2 cut(s) 120, 721
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 1 cut(s) 631
Ama87I CYCGRG 1 cut(s) 417
AoxI GGCC 2 cut(s) 537, 700
ApeKI GCWGC 2 cut(s) 303, 744
ApoI RAATTY 2 cut(s) 190, 770
Asp700I GAANNNNTTC 2 cut(s) 354, 434
AspS9I GGNCC 1 cut(s) 138
AsuC2I CCSGG 2 cut(s) 16, 483
AsuHPI GGTGA 1 cut(s) 562
AvaI CYCGRG 1 cut(s) 417
AvaII GGWCC 1 cut(s) 138
BaeGI GKGCMC 1 cut(s) 76
BalI TGGCCA 1 cut(s) 702
BbvI GCAGC 2 cut(s) 315, 731
BceAI ACGGC 2 cut(s) 46, 291
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 2 cut(s) 16, 483
BcoDI GTCTC 1 cut(s) 47
BisI GCNGC 4 cut(s) 304, 664, 694, 745
BlsI GCNGC 4 cut(s) 305, 665, 695, 746
Bme1390I CCNGG 2 cut(s) 16, 483
Bme18I GGWCC 1 cut(s) 138
BmeT110I CYCGRG 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 138
BmiI GGNNCC 1 cut(s) 487
BmrFI CCNGG 2 cut(s) 16, 483
BmsI GCATC 2 cut(s) 543, 670
BplI GAGNNNNNCTC 2 cut(s) 400, 432
BpuEI CTTGAG 2 cut(s) 605, 679
BpuMI CCSGG 2 cut(s) 16, 483
BsaJI CCNNGG 4 cut(s) 28, 230, 630, 668
Bsc4I CCNNNNNNNGG 1 cut(s) 670
BseDI CCNNGG 4 cut(s) 28, 230, 630, 668
BseGI GGATG 3 cut(s) 417, 560, 685
BseLI CCNNNNNNNGG 1 cut(s) 670
BseMII CTCAG 1 cut(s) 143
BseSI GKGCMC 1 cut(s) 76
BseXI GCAGC 2 cut(s) 315, 731
BshFI GGCC 2 cut(s) 539, 702
BsiHKCI CYCGRG 1 cut(s) 417
BsiSI CCGG 2 cut(s) 15, 482
BslI CCNNNNNNNGG 1 cut(s) 670
BsmAI GTCTC 1 cut(s) 47
BsnI GGCC 2 cut(s) 539, 702
BsoBI CYCGRG 1 cut(s) 417
Bsp1286I GDGCHC 1 cut(s) 76
Bsp143I GATC 3 cut(s) 3, 61, 623
Bsp19I CCATGG 1 cut(s) 630
BspACI CCGC 4 cut(s) 664, 693, 714, 742
BspANI GGCC 2 cut(s) 539, 702
BspCNI CTCAG 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 487
BspPI GGATC 1 cut(s) 631
BssECI CCNNGG 4 cut(s) 28, 230, 630, 668
BssMI GATC 3 cut(s) 3, 61, 623
BssT1I CCWWGG 2 cut(s) 630, 668
Bst4CI ACNGT 3 cut(s) 125, 231, 478
BstC8I GCNNGC 1 cut(s) 237
BstDEI CTNAG 1 cut(s) 152
BstDSI CCRYGG 3 cut(s) 28, 230, 630
BstF5I GGATG 3 cut(s) 417, 560, 685
BstKTI GATC 3 cut(s) 6, 64, 626
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 3 cut(s) 3, 61, 623
BstMWI GCNNNNNNNGC 3 cut(s) 276, 285, 699
BstNSI RCATGY 1 cut(s) 239
BstSCI CCNGG 2 cut(s) 14, 481
BstSLI GKGCMC 1 cut(s) 76
BstV1I GCAGC 2 cut(s) 315, 731
BstX2I RGATCY 1 cut(s) 623
BstYI RGATCY 1 cut(s) 623
BsuRI GGCC 2 cut(s) 539, 702
BtgI CCRYGG 3 cut(s) 28, 230, 630
BtsCI GGATG 3 cut(s) 417, 560, 685
Cac8I GCNNGC 1 cut(s) 237
Cfr13I GGNCC 1 cut(s) 138
Csp6I GTAC 1 cut(s) 496
CviAII CATG 8 cut(s) 98, 236, 382, 457, 467, 580, 631, 749
CviJI RGCY 8 cut(s) 120, 177, 306, 488, 539, 663, 702, 721
CviKI_1 RGCY 8 cut(s) 120, 177, 306, 488, 539, 663, 702, 721
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 1 cut(s) 152
DpnI GATC 3 cut(s) 5, 63, 625
DpnII GATC 3 cut(s) 3, 61, 623
EaeI YGGCCR 1 cut(s) 700
Eco130I CCWWGG 2 cut(s) 630, 668
Eco147I AGGCCT 1 cut(s) 539
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 138
Eco88I CYCGRG 1 cut(s) 417
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 2 cut(s) 630, 668
ErhI CCWWGG 2 cut(s) 630, 668
FaeI CATG 8 cut(s) 101, 239, 385, 460, 470, 583, 634, 752
FatI CATG 8 cut(s) 97, 235, 381, 456, 466, 579, 630, 748
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 753
Fnu4HI GCNGC 4 cut(s) 304, 664, 694, 745
FokI GGATG 3 cut(s) 424, 547, 692
Fsp4HI GCNGC 4 cut(s) 304, 664, 694, 745
GluI GCNGC 4 cut(s) 304, 664, 694, 745
HaeIII GGCC 2 cut(s) 539, 702
HapII CCGG 2 cut(s) 15, 482
Hin1II CATG 8 cut(s) 101, 239, 385, 460, 470, 583, 634, 752
HinfI GANTC 4 cut(s) 385, 415, 576, 783
HpaII CCGG 2 cut(s) 15, 482
HphI GGTGA 1 cut(s) 562
Hpy166II GTNNAC 3 cut(s) 322, 379, 754
Hpy188I TCNGA 3 cut(s) 623, 776, 788
Hpy188III TCNNGA 4 cut(s) 59, 419, 550, 658
Hpy8I GTNNAC 3 cut(s) 322, 379, 754
HpyAV CCTTC 4 cut(s) 151, 265, 550, 616
HpyCH4III ACNGT 3 cut(s) 125, 231, 478
HpyCH4IV ACGT 2 cut(s) 163, 224
HpyCH4V TGCA 4 cut(s) 97, 303, 683, 795
HpyF10VI GCNNNNNNNGC 3 cut(s) 276, 285, 699
HpyF3I CTNAG 1 cut(s) 152
HpySE526I ACGT 2 cut(s) 163, 224
Hsp92II CATG 8 cut(s) 101, 239, 385, 460, 470, 583, 634, 752
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 61, 623
LmnI GCTCC 4 cut(s) 101, 244, 485, 726
LpnPI CCDG 4 cut(s) 28, 164, 495, 586
Lsp1109I GCAGC 2 cut(s) 315, 731
LweI GCATC 2 cut(s) 543, 670
MaeII ACGT 2 cut(s) 163, 224
MaeIII GTNAC 3 cut(s) 22, 225, 472
MalI GATC 3 cut(s) 5, 63, 625
MboI GATC 3 cut(s) 3, 61, 623
MboII GAAGA 4 cut(s) 538, 618, 754, 757
MfeI CAATTG 1 cut(s) 65
MflI RGATCY 1 cut(s) 623
MhlI GDGCHC 1 cut(s) 76
MlsI TGGCCA 1 cut(s) 702
MluCI AATT 5 cut(s) 65, 190, 326, 490, 770
MluNI TGGCCA 1 cut(s) 702
MlyI GAGTC 3 cut(s) 409, 585, 777
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 4 cut(s) 61, 221, 369, 402
Mox20I TGGCCA 1 cut(s) 702
MroXI GAANNNNTTC 2 cut(s) 354, 434
MscI TGGCCA 1 cut(s) 702
MslI CAYNNNNRTG 1 cut(s) 688
Msp20I TGGCCA 1 cut(s) 702
MspA1I CMGCKG 1 cut(s) 744
MspI CCGG 2 cut(s) 15, 482
MspR9I CCNGG 2 cut(s) 16, 483
MunI CAATTG 1 cut(s) 65
MwoI GCNNNNNNNGC 3 cut(s) 276, 285, 699
NciI CCSGG 2 cut(s) 16, 483
NcoI CCATGG 1 cut(s) 630
NdeII GATC 3 cut(s) 3, 61, 623
NlaIII CATG 8 cut(s) 101, 239, 385, 460, 470, 583, 634, 752
NlaIV GGNNCC 1 cut(s) 487
NmeAIII GCCGAG 1 cut(s) 624
NmuCI GTSAC 1 cut(s) 472
NspI RCATGY 1 cut(s) 239
OliI CACNNNNGTG 1 cut(s) 688
PaeI GCATGC 1 cut(s) 239
PaeR7I CTCGAG 1 cut(s) 417
PceI AGGCCT 1 cut(s) 539
PdmI GAANNNNTTC 2 cut(s) 354, 434
PfeI GAWTC 1 cut(s) 385
PfoI TCCNGGA 1 cut(s) 481
PkrI GCNGC 4 cut(s) 305, 665, 695, 746
PleI GAGTC 3 cut(s) 409, 584, 777
PpsI GAGTC 3 cut(s) 409, 584, 777
PspN4I GGNNCC 1 cut(s) 487
PspPI GGNCC 1 cut(s) 138
PsrI GAACNNNNNNTAC 2 cut(s) 339, 371
PsuI RGATCY 1 cut(s) 623
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
RseI CAYNNNNRTG 1 cut(s) 688
SatI GCNGC 4 cut(s) 304, 664, 694, 745
Sau3AI GATC 3 cut(s) 3, 61, 623
Sau96I GGNCC 1 cut(s) 138
SchI GAGTC 3 cut(s) 409, 585, 777
ScrFI CCNGG 2 cut(s) 16, 483
SduI GDGCHC 1 cut(s) 76
SetI ASST 9 cut(s) 122, 143, 153, 166, 174, 213, 227, 257, 723
SfaNI GCATC 2 cut(s) 543, 670
Sfr274I CTCGAG 1 cut(s) 417
SinI GGWCC 1 cut(s) 138
SlaI CTCGAG 1 cut(s) 417
SmiMI CAYNNNNRTG 1 cut(s) 688
SmlI CTYRAG 3 cut(s) 417, 584, 658
SmoI CTYRAG 3 cut(s) 417, 584, 658
SphI GCATGC 1 cut(s) 239
Sse9I AATT 5 cut(s) 65, 190, 326, 490, 770
SseBI AGGCCT 1 cut(s) 539
SsiI CCGC 4 cut(s) 664, 693, 714, 742
StuI AGGCCT 1 cut(s) 539
StyD4I CCNGG 2 cut(s) 14, 481
StyI CCWWGG 2 cut(s) 630, 668
TaaI ACNGT 3 cut(s) 125, 231, 478
TaiI ACGT 2 cut(s) 166, 227
TaqI TCGA 4 cut(s) 60, 281, 418, 639
TasI AATT 5 cut(s) 65, 190, 326, 490, 770
TauI GCSGC 2 cut(s) 666, 696
TfiI GAWTC 1 cut(s) 385
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 2 cut(s) 303, 744
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 4 cut(s) 354, 398, 445, 532
TspGWI ACGGA 2 cut(s) 71, 795
VpaK11BI GGWCC 1 cut(s) 138
XapI RAATTY 2 cut(s) 190, 770
XceI RCATGY 1 cut(s) 239
XhoI CTCGAG 1 cut(s) 417
XmiI GTMKAC 1 cut(s) 753
XmnI GAANNNNTTC 2 cut(s) 354, 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.