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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 65 |
| AccB1I | GGYRCC | 1 cut(s) | 172 |
| AccI | GTMKAC | 2 cut(s) | 53, 182 |
| AcsI | RAATTY | 1 cut(s) | 157 |
| AcyI | GRCGYC | 1 cut(s) | 29 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 270 |
| AgsI | TTSAA | 2 cut(s) | 40, 48 |
| AjnI | CCWGG | 1 cut(s) | 23 |
| AoxI | GGCC | 3 cut(s) | 3, 201, 217 |
| ApoI | RAATTY | 1 cut(s) | 157 |
| AspLEI | GCGC | 1 cut(s) | 62 |
| AspS9I | GGNCC | 1 cut(s) | 201 |
| BanI | GGYRCC | 1 cut(s) | 172 |
| BbsI | GAAGAC | 1 cut(s) | 199 |
| BccI | CCATC | 2 cut(s) | 7, 160 |
| BceAI | ACGGC | 1 cut(s) | 188 |
| BciT130I | CCWGG | 1 cut(s) | 25 |
| BfaI | CTAG | 1 cut(s) | 270 |
| BfuAI | ACCTGC | 1 cut(s) | 65 |
| Bme1390I | CCNGG | 1 cut(s) | 25 |
| BmgT120I | GGNCC | 1 cut(s) | 201 |
| BmiI | GGNNCC | 1 cut(s) | 174 |
| BmrFI | CCNGG | 1 cut(s) | 25 |
| BpiI | GAAGAC | 1 cut(s) | 199 |
| Bsa29I | ATCGAT | 1 cut(s) | 71 |
| BsaHI | GRCGYC | 1 cut(s) | 29 |
| BsaJI | CCNNGG | 2 cut(s) | 24, 224 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 270 |
| BseBI | CCWGG | 1 cut(s) | 25 |
| BseCI | ATCGAT | 1 cut(s) | 71 |
| BseDI | CCNNGG | 2 cut(s) | 24, 224 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 270 |
| BshFI | GGCC | 3 cut(s) | 5, 203, 219 |
| BshNI | GGYRCC | 1 cut(s) | 172 |
| BshVI | ATCGAT | 1 cut(s) | 71 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 270 |
| BsnI | GGCC | 3 cut(s) | 5, 203, 219 |
| BspANI | GGCC | 3 cut(s) | 5, 203, 219 |
| BspDI | ATCGAT | 1 cut(s) | 71 |
| BspHI | TCATGA | 1 cut(s) | 210 |
| BspLI | GGNNCC | 1 cut(s) | 174 |
| BspMI | ACCTGC | 1 cut(s) | 65 |
| BspT107I | GGYRCC | 1 cut(s) | 172 |
| BssECI | CCNNGG | 2 cut(s) | 24, 224 |
| BssNAI | GTATAC | 2 cut(s) | 54, 183 |
| BssNI | GRCGYC | 1 cut(s) | 29 |
| BssT1I | CCWWGG | 1 cut(s) | 224 |
| Bst1107I | GTATAC | 2 cut(s) | 54, 183 |
| Bst2UI | CCWGG | 1 cut(s) | 25 |
| BstACI | GRCGYC | 1 cut(s) | 29 |
| BstDEI | CTNAG | 1 cut(s) | 7 |
| BstHHI | GCGC | 1 cut(s) | 62 |
| BstNI | CCWGG | 1 cut(s) | 25 |
| BstSCI | CCNGG | 1 cut(s) | 23 |
| BstV2I | GAAGAC | 1 cut(s) | 199 |
| BstZ17I | GTATAC | 2 cut(s) | 54, 183 |
| Bsu15I | ATCGAT | 1 cut(s) | 71 |
| BsuRI | GGCC | 3 cut(s) | 5, 203, 219 |
| BsuTUI | ATCGAT | 1 cut(s) | 71 |
| BveI | ACCTGC | 1 cut(s) | 65 |
| CciI | TCATGA | 1 cut(s) | 210 |
| CfoI | GCGC | 1 cut(s) | 62 |
| Cfr13I | GGNCC | 1 cut(s) | 201 |
| ClaI | ATCGAT | 1 cut(s) | 71 |
| CseI | GACGC | 1 cut(s) | 18 |
| CviAII | CATG | 2 cut(s) | 211, 265 |
| CviJI | RGCY | 4 cut(s) | 5, 203, 219, 254 |
| CviKI_1 | RGCY | 4 cut(s) | 5, 203, 219, 254 |
| DdeI | CTNAG | 1 cut(s) | 7 |
| Eco130I | CCWWGG | 1 cut(s) | 224 |
| Eco32I | GATATC | 1 cut(s) | 113 |
| EcoRI | GAATTC | 1 cut(s) | 157 |
| EcoRII | CCWGG | 1 cut(s) | 23 |
| EcoRV | GATATC | 1 cut(s) | 113 |
| EcoT14I | CCWWGG | 1 cut(s) | 224 |
| ErhI | CCWWGG | 1 cut(s) | 224 |
| FaeI | CATG | 2 cut(s) | 214, 268 |
| FaiI | YATR | 5 cut(s) | 54, 119, 183, 212, 266 |
| FatI | CATG | 2 cut(s) | 210, 264 |
| FblI | GTMKAC | 2 cut(s) | 53, 182 |
| FspBI | CTAG | 1 cut(s) | 270 |
| GlaI | GCGC | 1 cut(s) | 61 |
| HaeIII | GGCC | 3 cut(s) | 5, 203, 219 |
| HgaI | GACGC | 1 cut(s) | 18 |
| HhaI | GCGC | 1 cut(s) | 62 |
| Hin1I | GRCGYC | 1 cut(s) | 29 |
| Hin1II | CATG | 2 cut(s) | 214, 268 |
| Hin6I | GCGC | 1 cut(s) | 60 |
| HinP1I | GCGC | 1 cut(s) | 60 |
| HinfI | GANTC | 3 cut(s) | 68, 231, 257 |
| Hpy166II | GTNNAC | 2 cut(s) | 54, 183 |
| Hpy188I | TCNGA | 1 cut(s) | 127 |
| Hpy188III | TCNNGA | 1 cut(s) | 211 |
| Hpy8I | GTNNAC | 2 cut(s) | 54, 183 |
| HpyCH4IV | ACGT | 1 cut(s) | 275 |
| HpyF3I | CTNAG | 1 cut(s) | 7 |
| HpySE526I | ACGT | 1 cut(s) | 275 |
| Hsp92I | GRCGYC | 1 cut(s) | 29 |
| Hsp92II | CATG | 2 cut(s) | 214, 268 |
| HspAI | GCGC | 1 cut(s) | 60 |
| LpnPI | CCDG | 3 cut(s) | 10, 37, 70 |
| MaeI | CTAG | 1 cut(s) | 270 |
| MaeII | ACGT | 1 cut(s) | 275 |
| MboII | GAAGA | 3 cut(s) | 36, 184, 199 |
| MluCI | AATT | 2 cut(s) | 40, 157 |
| MnlI | CCTC | 2 cut(s) | 43, 271 |
| MseI | TTAA | 1 cut(s) | 134 |
| MspR9I | CCNGG | 1 cut(s) | 25 |
| MvaI | CCWGG | 1 cut(s) | 25 |
| NlaIII | CATG | 2 cut(s) | 214, 268 |
| NlaIV | GGNNCC | 1 cut(s) | 174 |
| PagI | TCATGA | 1 cut(s) | 210 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 201 |
| PfeI | GAWTC | 3 cut(s) | 68, 231, 257 |
| Psp6I | CCWGG | 1 cut(s) | 23 |
| PspGI | CCWGG | 1 cut(s) | 23 |
| PspN4I | GGNNCC | 1 cut(s) | 174 |
| PspPI | GGNCC | 1 cut(s) | 201 |
| SaqAI | TTAA | 1 cut(s) | 134 |
| Sau96I | GGNCC | 1 cut(s) | 201 |
| ScrFI | CCNGG | 1 cut(s) | 25 |
| SetI | ASST | 4 cut(s) | 53, 59, 174, 278 |
| Sse9I | AATT | 2 cut(s) | 40, 157 |
| SspI | AATATT | 1 cut(s) | 132 |
| SspMI | CTAG | 1 cut(s) | 270 |
| StyD4I | CCNGG | 1 cut(s) | 23 |
| StyI | CCWWGG | 1 cut(s) | 224 |
| TaiI | ACGT | 1 cut(s) | 278 |
| TaqI | TCGA | 2 cut(s) | 71, 155 |
| TasI | AATT | 2 cut(s) | 40, 157 |
| TfiI | GAWTC | 3 cut(s) | 68, 231, 257 |
| Tru1I | TTAA | 1 cut(s) | 134 |
| Tru9I | TTAA | 1 cut(s) | 134 |
| TspDTI | ATGAA | 2 cut(s) | 199, 227 |
| XapI | RAATTY | 1 cut(s) | 157 |
| XmiI | GTMKAC | 2 cut(s) | 53, 182 |
| XspI | CTAG | 1 cut(s) | 270 |