pycom01g01720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001044
Physical Location & Seq
Forward (+)
1589322 .. 1591253
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g01720.2

Sequence Viewer

Length: 1602 bp
ATGCATGTGGTGGGACTAATACCTTTGATATCGGCCAATGTCCAACCTAGGGCAGATTTGAATTCCTTCAAAACACGCACTAGTTTCTCCTCTTCTTGTGCCGTGAGTGATGAAGAAATGATGGCCGGTAATGTCTCCTTTTCTCCCAAGAAAATAGATGGAAGCAATTTGTTAGTCGAAATGGGAATGGACTCACGGTTAGTATACTTACCATCAAGCTTAGGTGAGGATTCAAGGGCAGCCACAACTTCAAGTAATTCCTCACTAGGGGGCACGGCATGGGATGACTCTTGTATGCCGTGGGCATGCATGCACTCTTCCTCCTTTGTTTTGAGTTCCATGCCTCGTGTAATGACTTTTTCAAGCGCATCGTCATTCAAATCGTCGAGATATCCCTGCGCCAAAGAGTCAATTATATCAATAGAAAAGCACGAATGGTCCTCACTAGGGTATTTAATGGAATCAGAAAGATTAAAATTAACAACTTCCCCATCGAATTCCATGGACAAAGTTCCACTATACACGTCAATCTTCGTCCGGGCCGTCTTCATGAATGGCCTTCCAAGTAGAATGGGTAAAATCCGCCGGGAAGACCAAATGATTAACCTGCACAAGAACATCTTCCAAAACTCCCTTTGGATAGGCGTTAGATCTATCGGCCAATTGTATTATTACCCCATCATTTTTCAATTTATAGAAGCACCTAAATCAAGCATGGCAGATTCAAATCTAGTATTCCCAATGACACAAGGAATGGTAAAGCTACCTGATCGATCCTTGCATTTGGGGGGTAATTTGCGTTGCAAGATGGCGGAGACATTTTCACCTACCTTTACTACTTCTTTGGTCGCCATCCTCTTCCTTGTGGTACACAACTCCTTCAAGAACTTAGCATACCTCGGAACTTGCTTGATTGCATCTAACAAAGGAAAAGGAACATTCAAAGGAAAAGCATTAGTAGGAACTGAACTTGACACGTTTTTACCTTTATGGGGTGAATTGGACGGTTTAGGATCACTTGGAACTTGCGGCACATTTGGTTCCACCTTGGCCGTGAGTGGCCTTTGCTCCTCCTCTTCAATTCTCAACTCGTCCTCCTCGTTGGAACGTGATTTGGATGGTTTGAGGTCAGATCCGACTTGCTTTCCACTTCGCAAATGCATAGCTTTTGCAGATTCGAATCCACCTTTCGGATTGACCACCGTTGAGCTAGGCAACCTTCCTTGCTCACGAAATTGCCCCATGAACTCGGCAATCTGTCCCACTTGCTTCTCCAAGTTGTCCACCCTCTTGTCTTGGATTTGCCTCTCCTTTACCTACATTGCTTTGGGCAGGTTGTGCTTGGGCTTGTGGTGGTGCCAACGGCTTTTGATAGAAACCCGGGGGTTGTTGCCTAAATCCGCTTTGTTGTTGTCCTTGTTGGGGGTCTCGCCATTTGAAATTCGGATGATCACGCCAACCAGGATTGTAAATCCCACGGCATTGAGTGTCTCCCACCCTCCATTCTCGATTAATTGTGGGCACTTGTCCGTAGGGTGTCCTTGCATGGAGCATACGCCACAAGAAGCTACGGTTTTAACTTTTGGACCTTCCACCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

534

Amino Acids

57.99

Weight (kDa)

8.53

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 615, 1323
AccB1I GGYRCC 1 cut(s) 1356
AccI GTMKAC 1 cut(s) 204
AciI CCGC 4 cut(s) 583, 812, 1029, 1401
AclWI GGATC 3 cut(s) 768, 1021, 1127
AcoI YGGCCR 4 cut(s) 33, 123, 658, 1050
AcsI RAATTY 3 cut(s) 61, 496, 1440
AfaI GTAC 1 cut(s) 870
AfiI CCNNNNNNNGG 6 cut(s) 49, 267, 447, 992, 1190, 1422
AflIII ACRYGT 2 cut(s) 522, 975
AhlI ACTAGT 1 cut(s) 80
AjiI CACGTC 1 cut(s) 525
AjnI CCWGG 1 cut(s) 1460
AluBI AGCT 5 cut(s) 219, 763, 1166, 1210, 1568
AluI AGCT 5 cut(s) 219, 763, 1166, 1210, 1568
Alw26I GTCTC 4 cut(s) 139, 809, 1432, 1495
AlwI GGATC 3 cut(s) 768, 1021, 1127
Ama87I CYCGRG 1 cut(s) 1380
AoxI GGCC 7 cut(s) 33, 123, 540, 556, 658, 1050, 1060
ApeKI GCWGC 1 cut(s) 239
ApoI RAATTY 3 cut(s) 61, 496, 1440
AseI ATTAAT 1 cut(s) 1512
Asp700I GAANNNNTTC 2 cut(s) 65, 620
AspA2I CCTAGG 1 cut(s) 47
AspLEI GCGC 2 cut(s) 368, 401
AspS9I GGNCC 3 cut(s) 438, 540, 1586
AsuC2I CCSGG 4 cut(s) 539, 587, 1381, 1382
AsuHPI GGTGA 3 cut(s) 236, 816, 1007
AsuII TTCGAA 1 cut(s) 1178
AvaI CYCGRG 1 cut(s) 1380
AvaII GGWCC 2 cut(s) 438, 1586
AvrII CCTAGG 1 cut(s) 47
BaeGI GKGCMC 2 cut(s) 275, 1524
BanI GGYRCC 1 cut(s) 1356
BauI CACGAG 1 cut(s) 345
BbsI GAAGAC 2 cut(s) 538, 597
BbvI GCAGC 1 cut(s) 251
BccI CCATC 8 cut(s) 115, 152, 220, 499, 686, 802, 860, 1112
BceAI ACGGC 7 cut(s) 86, 283, 291, 527, 1037, 1379, 1494
BciT130I CCWGG 1 cut(s) 1462
BclI TGATCA 1 cut(s) 1449
BcnI CCSGG 4 cut(s) 539, 587, 1381, 1382
BcoDI GTCTC 4 cut(s) 139, 809, 1432, 1495
BcuI ACTAGT 1 cut(s) 80
BfaI CTAG 6 cut(s) 48, 81, 266, 446, 731, 1211
BfuAI ACCTGC 2 cut(s) 615, 1323
BglII AGATCT 1 cut(s) 650
BisI GCNGC 2 cut(s) 240, 1030
BlnI CCTAGG 1 cut(s) 47
BlsI GCNGC 2 cut(s) 241, 1031
Bme1390I CCNGG 5 cut(s) 539, 587, 1381, 1382, 1462
Bme18I GGWCC 2 cut(s) 438, 1586
BmeT110I CYCGRG 1 cut(s) 1380
BmgBI CACGTC 1 cut(s) 525
BmgT120I GGNCC 3 cut(s) 438, 540, 1586
BmiI GGNNCC 2 cut(s) 1042, 1358
BmrFI CCNGG 5 cut(s) 539, 587, 1381, 1382, 1462
BmsI GCATC 2 cut(s) 377, 926
BpiI GAAGAC 2 cut(s) 538, 597
Bpu10I CCTNAGC 1 cut(s) 220
Bpu14I TTCGAA 1 cut(s) 1178
BpuMI CCSGG 4 cut(s) 539, 587, 1381, 1382
Bsa29I ATCGAT 1 cut(s) 772
BsaBI GATNNNNATC 2 cut(s) 726, 1179
BsaI GGTCTC 1 cut(s) 1432
BsaJI CCNNGG 8 cut(s) 47, 299, 501, 898, 1047, 1380, 1381, 1476
BsaXI ACNNNNNCTCC 4 cut(s) 305, 335, 1079, 1109
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 267, 447, 992, 1190, 1422
Bse118I RCCGGY 1 cut(s) 125
Bse3DI GCAATG 1 cut(s) 1320
Bse8I GATNNNNATC 2 cut(s) 726, 1179
BseBI CCWGG 1 cut(s) 1462
BseCI ATCGAT 1 cut(s) 772
BseDI CCNNGG 8 cut(s) 47, 299, 501, 898, 1047, 1380, 1381, 1476
BseGI GGATG 4 cut(s) 289, 852, 1123, 1452
BseJI GATNNNNATC 2 cut(s) 726, 1179
BseLI CCNNNNNNNGG 6 cut(s) 49, 267, 447, 992, 1190, 1422
BseMI GCAATG 1 cut(s) 1320
BseRI GAGGAG 4 cut(s) 79, 1060, 1063, 1087
BseSI GKGCMC 2 cut(s) 275, 1524
BseXI GCAGC 1 cut(s) 251
BsgI GTGCAG 1 cut(s) 593
BshFI GGCC 7 cut(s) 35, 125, 542, 558, 660, 1052, 1062
BshNI GGYRCC 1 cut(s) 1356
BshVI ATCGAT 1 cut(s) 772
BsiHKCI CYCGRG 1 cut(s) 1380
BsiSI CCGG 4 cut(s) 126, 538, 586, 1381
BslFI GGGAC 2 cut(s) 27, 1245
BslI CCNNNNNNNGG 6 cut(s) 49, 267, 447, 992, 1190, 1422
BsmAI GTCTC 4 cut(s) 139, 809, 1432, 1495
BsmFI GGGAC 2 cut(s) 27, 1245
BsnI GGCC 7 cut(s) 35, 125, 542, 558, 660, 1052, 1062
Bso31I GGTCTC 1 cut(s) 1432
BsoBI CYCGRG 1 cut(s) 1380
Bsp119I TTCGAA 1 cut(s) 1178
Bsp1286I GDGCHC 2 cut(s) 275, 1524
Bsp143I GATC 6 cut(s) 650, 769, 773, 1013, 1132, 1449
Bsp19I CCATGG 1 cut(s) 501
BspACI CCGC 4 cut(s) 583, 812, 1029, 1401
BspANI GGCC 7 cut(s) 35, 125, 542, 558, 660, 1052, 1062
BspDI ATCGAT 1 cut(s) 772
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 2 cut(s) 1042, 1358
BspMI ACCTGC 2 cut(s) 615, 1323
BspPI GGATC 3 cut(s) 768, 1021, 1127
BspT104I TTCGAA 1 cut(s) 1178
BspT107I GGYRCC 1 cut(s) 1356
BspTNI GGTCTC 1 cut(s) 1432
BsrDI GCAATG 1 cut(s) 1320
BsrFI RCCGGY 1 cut(s) 125
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 8 cut(s) 47, 299, 501, 898, 1047, 1380, 1381, 1476
BssMI GATC 6 cut(s) 650, 769, 773, 1013, 1132, 1449
BssNAI GTATAC 1 cut(s) 205
BssSI CACGAG 1 cut(s) 345
BssT1I CCWWGG 3 cut(s) 47, 501, 1047
Bst1107I GTATAC 1 cut(s) 205
Bst2BI CACGAG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 1462
Bst4CI ACNGT 4 cut(s) 198, 1007, 1204, 1573
Bst6I CTCTTC 4 cut(s) 97, 322, 863, 1081
BstAPI GCANNNNNTGC 1 cut(s) 1338
BstBI TTCGAA 1 cut(s) 1178
BstC8I GCNNGC 2 cut(s) 307, 311
BstDEI CTNAG 2 cut(s) 220, 889
BstDSI CCRYGG 3 cut(s) 299, 501, 1476
BstF5I GGATG 4 cut(s) 289, 852, 1123, 1452
BstHHI GCGC 2 cut(s) 368, 401
BstKTI GATC 6 cut(s) 653, 772, 776, 1016, 1135, 1452
BstMAI GTCTC 4 cut(s) 139, 809, 1432, 1495
BstMBI GATC 6 cut(s) 650, 769, 773, 1013, 1132, 1449
BstMWI GCNNNNNNNGC 1 cut(s) 1338
BstNI CCWGG 1 cut(s) 1462
BstNSI RCATGY 3 cut(s) 8, 309, 313
BstSCI CCNGG 5 cut(s) 537, 585, 1379, 1380, 1460
BstSLI GKGCMC 2 cut(s) 275, 1524
BstV1I GCAGC 1 cut(s) 251
BstV2I GAAGAC 2 cut(s) 538, 597
BstX2I RGATCY 2 cut(s) 650, 1132
BstYI RGATCY 2 cut(s) 650, 1132
BstZ17I GTATAC 1 cut(s) 205
Bsu15I ATCGAT 1 cut(s) 772
BsuRI GGCC 7 cut(s) 35, 125, 542, 558, 660, 1052, 1062
BsuTUI ATCGAT 1 cut(s) 772
BtgI CCRYGG 3 cut(s) 299, 501, 1476
BtrI CACGTC 1 cut(s) 525
BtsCI GGATG 4 cut(s) 289, 852, 1123, 1452
BveI ACCTGC 2 cut(s) 615, 1323
Cac8I GCNNGC 2 cut(s) 307, 311
CciI TCATGA 1 cut(s) 549
CfoI GCGC 2 cut(s) 368, 401
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 3 cut(s) 438, 540, 1586
Cfr9I CCCGGG 1 cut(s) 1380
ClaI ATCGAT 1 cut(s) 772
Csp6I GTAC 1 cut(s) 869
CviQI GTAC 1 cut(s) 869
DdeI CTNAG 2 cut(s) 220, 889
DpnI GATC 6 cut(s) 652, 771, 775, 1015, 1134, 1451
DpnII GATC 6 cut(s) 650, 769, 773, 1013, 1132, 1449
EaeI YGGCCR 4 cut(s) 33, 123, 658, 1050
Eam1104I CTCTTC 4 cut(s) 97, 322, 863, 1081
EarI CTCTTC 4 cut(s) 97, 322, 863, 1081
EciI GGCGGA 2 cut(s) 572, 827
Eco130I CCWWGG 3 cut(s) 47, 501, 1047
Eco31I GGTCTC 1 cut(s) 1432
Eco32I GATATC 2 cut(s) 30, 392
Eco47I GGWCC 2 cut(s) 438, 1586
Eco88I CYCGRG 1 cut(s) 1380
EcoRI GAATTC 2 cut(s) 61, 496
EcoRII CCWGG 1 cut(s) 1460
EcoRV GATATC 2 cut(s) 30, 392
EcoT14I CCWWGG 3 cut(s) 47, 501, 1047
EcoT22I ATGCAT 3 cut(s) 6, 311, 1163
ErhI CCWWGG 3 cut(s) 47, 501, 1047
FalI AAGNNNNNCTT 2 cut(s) 605, 637
FaqI GGGAC 2 cut(s) 27, 1245
FbaI TGATCA 1 cut(s) 1449
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 240, 1030
FokI GGATG 4 cut(s) 296, 839, 1130, 1459
Fsp4HI GCNGC 2 cut(s) 240, 1030
FspBI CTAG 6 cut(s) 48, 81, 266, 446, 731, 1211
GlaI GCGC 2 cut(s) 367, 400
GluI GCNGC 2 cut(s) 240, 1030
HaeIII GGCC 7 cut(s) 35, 125, 542, 558, 660, 1052, 1062
HapII CCGG 4 cut(s) 126, 538, 586, 1381
HhaI GCGC 2 cut(s) 368, 401
Hin6I GCGC 2 cut(s) 366, 399
HinP1I GCGC 2 cut(s) 366, 399
HindIII AAGCTT 1 cut(s) 217
HinfI GANTC 8 cut(s) 191, 230, 287, 407, 461, 722, 1175, 1180
HpaII CCGG 4 cut(s) 126, 538, 586, 1381
HphI GGTGA 3 cut(s) 236, 816, 1007
Hpy166II GTNNAC 3 cut(s) 205, 871, 1284
Hpy188I TCNGA 6 cut(s) 466, 902, 1132, 1137, 1193, 1446
Hpy188III TCNNGA 5 cut(s) 387, 550, 883, 1230, 1507
Hpy8I GTNNAC 3 cut(s) 205, 871, 1284
Hpy99I CGWCG 1 cut(s) 388
HpyAV CCTTC 5 cut(s) 76, 569, 889, 1229, 1599
HpyCH4III ACNGT 4 cut(s) 198, 1007, 1204, 1573
HpyCH4IV ACGT 3 cut(s) 524, 977, 1108
HpyF10VI GCNNNNNNNGC 1 cut(s) 1338
HpyF3I CTNAG 2 cut(s) 220, 889
HpySE526I ACGT 3 cut(s) 524, 977, 1108
HspAI GCGC 2 cut(s) 366, 399
Ksp22I TGATCA 1 cut(s) 1449
Kzo9I GATC 6 cut(s) 650, 769, 773, 1013, 1132, 1449
LmnI GCTCC 2 cut(s) 1073, 1549
Lsp1109I GCAGC 1 cut(s) 251
LweI GCATC 2 cut(s) 377, 926
MaeI CTAG 6 cut(s) 48, 81, 266, 446, 731, 1211
MaeII ACGT 3 cut(s) 524, 977, 1108
MalI GATC 6 cut(s) 652, 771, 775, 1015, 1134, 1451
MboI GATC 6 cut(s) 650, 769, 773, 1013, 1132, 1449
MboII GAAGA 9 cut(s) 84, 125, 309, 523, 538, 602, 613, 850, 1068
MfeI CAATTG 1 cut(s) 662
MflI RGATCY 2 cut(s) 650, 1132
MhlI GDGCHC 2 cut(s) 275, 1524
MlyI GAGTC 3 cut(s) 185, 281, 416
MmeI TCCRAC 3 cut(s) 67, 1083, 1160
Mph1103I ATGCAT 3 cut(s) 6, 311, 1163
MroXI GAANNNNTTC 2 cut(s) 65, 620
MseI TTAA 6 cut(s) 455, 473, 479, 603, 1512, 1577
MspI CCGG 4 cut(s) 126, 538, 586, 1381
MspR9I CCNGG 5 cut(s) 539, 587, 1381, 1382, 1462
MunI CAATTG 1 cut(s) 662
MvaI CCWGG 1 cut(s) 1462
MwoI GCNNNNNNNGC 1 cut(s) 1338
NciI CCSGG 4 cut(s) 539, 587, 1381, 1382
NcoI CCATGG 1 cut(s) 501
NdeII GATC 6 cut(s) 650, 769, 773, 1013, 1132, 1449
NlaIV GGNNCC 2 cut(s) 1042, 1358
NmeAIII GCCGAG 1 cut(s) 1229
NsiI ATGCAT 3 cut(s) 6, 311, 1163
NspI RCATGY 3 cut(s) 8, 309, 313
NspV TTCGAA 1 cut(s) 1178
PaeI GCATGC 2 cut(s) 309, 313
PagI TCATGA 1 cut(s) 549
PcsI WCGNNNNNNNCGW 2 cut(s) 540, 1097
PdmI GAANNNNTTC 2 cut(s) 65, 620
PfeI GAWTC 5 cut(s) 230, 461, 722, 1175, 1180
PkrI GCNGC 2 cut(s) 241, 1031
PleI GAGTC 3 cut(s) 185, 281, 415
PpsI GAGTC 3 cut(s) 185, 281, 415
PshBI ATTAAT 1 cut(s) 1512
Psp6I CCWGG 1 cut(s) 1460
PspGI CCWGG 1 cut(s) 1460
PspN4I GGNNCC 2 cut(s) 1042, 1358
PspPI GGNCC 3 cut(s) 438, 540, 1586
PsrI GAACNNNNNNTAC 2 cut(s) 878, 910
PsuI RGATCY 2 cut(s) 650, 1132
RsaI GTAC 1 cut(s) 870
RsaNI GTAC 1 cut(s) 869
SaqAI TTAA 6 cut(s) 455, 473, 479, 603, 1512, 1577
SatI GCNGC 2 cut(s) 240, 1030
Sau3AI GATC 6 cut(s) 650, 769, 773, 1013, 1132, 1449
Sau96I GGNCC 3 cut(s) 438, 540, 1586
SchI GAGTC 3 cut(s) 185, 281, 416
ScrFI CCNGG 5 cut(s) 539, 587, 1381, 1382, 1462
SduI GDGCHC 2 cut(s) 275, 1524
SfaNI GCATC 2 cut(s) 377, 926
SfuI TTCGAA 1 cut(s) 1178
SinI GGWCC 2 cut(s) 438, 1586
SmaI CCCGGG 1 cut(s) 1382
SpeI ACTAGT 1 cut(s) 80
SphI GCATGC 2 cut(s) 309, 313
SsiI CCGC 4 cut(s) 583, 812, 1029, 1401
SspMI CTAG 6 cut(s) 48, 81, 266, 446, 731, 1211
StyD4I CCNGG 5 cut(s) 537, 585, 1379, 1380, 1460
StyI CCWWGG 3 cut(s) 47, 501, 1047
TaaI ACNGT 4 cut(s) 198, 1007, 1204, 1573
TaiI ACGT 3 cut(s) 527, 980, 1111
TaqI TCGA 6 cut(s) 177, 386, 494, 772, 1178, 1508
TauI GCSGC 1 cut(s) 1032
TfiI GAWTC 5 cut(s) 230, 461, 722, 1175, 1180
Tru1I TTAA 6 cut(s) 455, 473, 479, 603, 1512, 1577
Tru9I TTAA 6 cut(s) 455, 473, 479, 603, 1512, 1577
TseI GCWGC 1 cut(s) 239
TspDTI ATGAA 4 cut(s) 126, 538, 566, 1259
TspGWI ACGGA 1 cut(s) 1519
TspMI CCCGGG 1 cut(s) 1380
VpaK11BI GGWCC 2 cut(s) 438, 1586
VspI ATTAAT 1 cut(s) 1512
XapI RAATTY 3 cut(s) 61, 496, 1440
XceI RCATGY 3 cut(s) 8, 309, 313
XmaI CCCGGG 1 cut(s) 1380
XmaJI CCTAGG 1 cut(s) 47
XmiI GTMKAC 1 cut(s) 204
XmnI GAANNNNTTC 2 cut(s) 65, 620
XspI CTAG 6 cut(s) 48, 81, 266, 446, 731, 1211
Zsp2I ATGCAT 3 cut(s) 6, 311, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.