pycom13g24360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001044
Physical Location & Seq
Forward (+)
21524547 .. 21525519
973 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g24360.1

Sequence Viewer

Length: 813 bp
ATGACTCGTGATGTCGGAAAGAACTTCTCTAAGAAGGCTCGCTTCATACTCTCCCATGATGTGACAGTTCCGGGAGCCAACTCATACAACCAATCCTTGGCCTTCTCCACCAAAGAAAAAGGAAAAGCCTTCATCTTCAATATGTTGCTGTCCACGTTCACCGGTGTCATACTTGAGCACACCACCTCGAATTCCTTTAGATGCTTGTTCGGATCCTCCATGGCCAACCCATGGTATTTCGGAATATGGTGGAGCAAGCTGGACTTCAACTCAAATTCATCGGTCTTCTCAGGGGCTGCCCTAGGGTATTGGATGCAAAGAGGTGCTGCATTGGCCAAACCCGAGGCAGAAAGCTCCTTGATGGTTCGATTGTCCACTGCCATGGTTGGTACTTCCTCTTCAACCCTTGCCGTGGCCTCCTCTTCAACCTCAACTTCAACTTCTTGCTCTTCTAATTCAGGTTCGGGACTAGGAGGATTAGACGCTTGCGATTTCTTTTTCCTTCTCAAAGTCCTCTCGAAATCACCGTCAAAGTCGGAGATATGCTCACGAACAGCAAACAAGGTTAGCAATCAATCTAATAGACTCGGCACAGGTAAGGAAATAAGGTGCCACACACGGCACAAAAAAAAACAAAACACACACACACGGTCCCTGGCAACGGCGCCAAAATTTGATGTGAAAATTATGTTGACACACAAATTAAACCCTATTTGTGACAATTGTAGTAATGAAGTAAGTAGGGATCGTTCTAACCGGGGATTAACTAGGGGTTGGGACGAATTTGAAAGTGTTATGACTCCAATTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

271

Amino Acids

29.48

Weight (kDa)

9.75

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 609, 664
AccB7I CCANNNNNTGG 2 cut(s) 97, 231
AclWI GGATC 3 cut(s) 207, 220, 753
AcoI YGGCCR 2 cut(s) 222, 333
AcsI RAATTY 4 cut(s) 190, 274, 671, 782
AcyI GRCGYC 1 cut(s) 665
AfaI GTAC 1 cut(s) 391
AfiI CCNNNNNNNGG 4 cut(s) 97, 231, 412, 661
AgeI ACCGGT 1 cut(s) 161
AgsI TTSAA 6 cut(s) 139, 268, 402, 426, 438, 788
AjnI CCWGG 1 cut(s) 654
AluBI AGCT 2 cut(s) 259, 354
AluI AGCT 2 cut(s) 259, 354
Alw21I GWGCWC 1 cut(s) 180
AlwI GGATC 3 cut(s) 207, 220, 753
AlwNI CAGNNNCTG 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 4 cut(s) 99, 222, 333, 414
ApeKI GCWGC 2 cut(s) 296, 326
ApoI RAATTY 4 cut(s) 190, 274, 671, 782
ArsI GACNNNNNNTTYG 2 cut(s) 512, 544
AsiGI ACCGGT 1 cut(s) 161
AspA2I CCTAGG 1 cut(s) 301
AspLEI GCGC 1 cut(s) 667
AspS9I GGNCC 1 cut(s) 651
AsuC2I CCSGG 2 cut(s) 72, 758
AsuHPI GGTGA 2 cut(s) 151, 516
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 1 cut(s) 651
AvrII CCTAGG 1 cut(s) 301
BalI TGGCCA 2 cut(s) 224, 335
BamHI GGATCC 1 cut(s) 212
BanI GGYRCC 2 cut(s) 609, 664
BarI GAAGNNNNNNTAC 2 cut(s) 382, 414
BauI CACGAG 1 cut(s) 6
BbsI GAAGAC 1 cut(s) 277
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 2 cut(s) 283, 313
BccI CCATC 1 cut(s) 355
BceAI ACGGC 3 cut(s) 395, 635, 678
BciT130I CCWGG 1 cut(s) 656
BcnI CCSGG 2 cut(s) 72, 758
BfaI CTAG 3 cut(s) 302, 470, 768
BfoI RGCGCY 1 cut(s) 668
BisI GCNGC 2 cut(s) 297, 327
BlnI CCTAGG 1 cut(s) 301
BlsI GCNGC 2 cut(s) 298, 328
Bme1390I CCNGG 3 cut(s) 72, 656, 758
Bme18I GGWCC 1 cut(s) 651
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 651
BmiI GGNNCC 5 cut(s) 76, 214, 611, 653, 666
BmrFI CCNGG 3 cut(s) 72, 656, 758
BmsI GCATC 2 cut(s) 191, 303
BpiI GAAGAC 1 cut(s) 277
BplI GAGNNNNNCTC 2 cut(s) 530, 562
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 2 cut(s) 72, 758
BsaHI GRCGYC 1 cut(s) 665
BsaJI CCNNGG 9 cut(s) 96, 219, 230, 301, 342, 381, 411, 654, 757
BsaWI WCCGGW 1 cut(s) 161
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 231, 412, 661
Bse118I RCCGGY 1 cut(s) 161
BseBI CCWGG 1 cut(s) 656
BseDI CCNNGG 9 cut(s) 96, 219, 230, 301, 342, 381, 411, 654, 757
BseGI GGATG 1 cut(s) 318
BseLI CCNNNNNNNGG 4 cut(s) 97, 231, 412, 661
BseMII CTCAG 1 cut(s) 303
BseRI GAGGAG 1 cut(s) 409
BseXI GCAGC 2 cut(s) 283, 313
BshFI GGCC 4 cut(s) 101, 224, 335, 416
BshNI GGYRCC 2 cut(s) 609, 664
BshTI ACCGGT 1 cut(s) 161
BsiHKAI GWGCWC 1 cut(s) 180
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 3 cut(s) 71, 162, 757
BslFI GGGAC 3 cut(s) 480, 637, 791
BslI CCNNNNNNNGG 4 cut(s) 97, 231, 412, 661
BsmFI GGGAC 3 cut(s) 480, 637, 791
BsnI GGCC 4 cut(s) 101, 224, 335, 416
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 2 cut(s) 212, 745
Bsp19I CCATGG 3 cut(s) 219, 230, 381
BspANI GGCC 4 cut(s) 101, 224, 335, 416
BspCNI CTCAG 1 cut(s) 302
BspLI GGNNCC 5 cut(s) 76, 214, 611, 653, 666
BspPI GGATC 3 cut(s) 207, 220, 753
BspQI GCTCTTC 1 cut(s) 454
BspT107I GGYRCC 2 cut(s) 609, 664
BsrFI RCCGGY 1 cut(s) 161
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 9 cut(s) 96, 219, 230, 301, 342, 381, 411, 654, 757
BssMI GATC 2 cut(s) 212, 745
BssNI GRCGYC 1 cut(s) 665
BssSI CACGAG 1 cut(s) 6
BssT1I CCWWGG 5 cut(s) 96, 219, 230, 301, 381
Bst2BI CACGAG 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 656
Bst4CI ACNGT 3 cut(s) 67, 528, 651
Bst6I CTCTTC 3 cut(s) 403, 427, 454
BstACI GRCGYC 1 cut(s) 665
BstC8I GCNNGC 3 cut(s) 40, 257, 487
BstDEI CTNAG 2 cut(s) 30, 289
BstDSI CCRYGG 4 cut(s) 219, 230, 381, 411
BstF5I GGATG 1 cut(s) 318
BstH2I RGCGCY 1 cut(s) 668
BstHHI GCGC 1 cut(s) 667
BstKTI GATC 2 cut(s) 215, 748
BstMBI GATC 2 cut(s) 212, 745
BstMWI GCNNNNNNNGC 1 cut(s) 332
BstNI CCWGG 1 cut(s) 656
BstSCI CCNGG 3 cut(s) 70, 654, 756
BstV1I GCAGC 2 cut(s) 283, 313
BstV2I GAAGAC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 212
BstXI CCANNNNNNTGG 1 cut(s) 382
BstYI RGATCY 1 cut(s) 212
BsuRI GGCC 4 cut(s) 101, 224, 335, 416
BtgI CCRYGG 4 cut(s) 219, 230, 381, 411
BtsCI GGATG 1 cut(s) 318
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 3 cut(s) 40, 257, 487
CaiI CAGNNNCTG 1 cut(s) 296
CfoI GCGC 1 cut(s) 667
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 1 cut(s) 651
CseI GACGC 1 cut(s) 491
Csp6I GTAC 1 cut(s) 390
CspAI ACCGGT 1 cut(s) 161
CviAII CATG 4 cut(s) 56, 220, 231, 382
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 2 cut(s) 30, 289
DinI GGCGCC 1 cut(s) 666
DpnI GATC 2 cut(s) 214, 747
DpnII GATC 2 cut(s) 212, 745
EaeI YGGCCR 2 cut(s) 222, 333
Eam1104I CTCTTC 3 cut(s) 403, 427, 454
EarI CTCTTC 3 cut(s) 403, 427, 454
Eco130I CCWWGG 5 cut(s) 96, 219, 230, 301, 381
Eco47I GGWCC 1 cut(s) 651
Eco88I CYCGRG 1 cut(s) 341
EcoRI GAATTC 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 654
EcoT14I CCWWGG 5 cut(s) 96, 219, 230, 301, 381
EgeI GGCGCC 1 cut(s) 666
EheI GGCGCC 1 cut(s) 666
ErhI CCWWGG 5 cut(s) 96, 219, 230, 301, 381
FaeI CATG 4 cut(s) 59, 223, 234, 385
FalI AAGNNNNNCTT 4 cut(s) 26, 58, 248, 280
FaqI GGGAC 3 cut(s) 480, 637, 791
FatI CATG 4 cut(s) 55, 219, 230, 381
Fnu4HI GCNGC 2 cut(s) 297, 327
FokI GGATG 1 cut(s) 325
Fsp4HI GCNGC 2 cut(s) 297, 327
FspBI CTAG 3 cut(s) 302, 470, 768
GlaI GCGC 1 cut(s) 666
GluI GCNGC 2 cut(s) 297, 327
HaeII RGCGCY 1 cut(s) 668
HaeIII GGCC 4 cut(s) 101, 224, 335, 416
HapII CCGG 3 cut(s) 71, 162, 757
HgaI GACGC 1 cut(s) 491
HhaI GCGC 1 cut(s) 667
Hin1I GRCGYC 1 cut(s) 665
Hin1II CATG 4 cut(s) 59, 223, 234, 385
Hin6I GCGC 1 cut(s) 665
HinP1I GCGC 1 cut(s) 665
HincII GTYRAC 1 cut(s) 693
HindII GTYRAC 1 cut(s) 693
HinfI GANTC 3 cut(s) 4, 585, 799
HpaII CCGG 3 cut(s) 71, 162, 757
HphI GGTGA 2 cut(s) 151, 516
Hpy166II GTNNAC 4 cut(s) 153, 159, 375, 693
Hpy188I TCNGA 4 cut(s) 17, 212, 242, 538
Hpy188III TCNNGA 4 cut(s) 8, 465, 517, 549
Hpy8I GTNNAC 4 cut(s) 153, 159, 375, 693
HpyAV CCTTC 4 cut(s) 28, 112, 139, 512
HpyCH4III ACNGT 3 cut(s) 67, 528, 651
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 2 cut(s) 316, 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 332
HpyF3I CTNAG 2 cut(s) 30, 289
HpySE526I ACGT 1 cut(s) 155
Hsp92I GRCGYC 1 cut(s) 665
Hsp92II CATG 4 cut(s) 59, 223, 234, 385
HspAI GCGC 1 cut(s) 665
KasI GGCGCC 1 cut(s) 664
Kzo9I GATC 2 cut(s) 212, 745
LguI GCTCTTC 1 cut(s) 454
LmnI GCTCC 3 cut(s) 74, 252, 359
LpnPI CCDG 9 cut(s) 84, 175, 245, 276, 444, 579, 641, 668, 770
Lsp1109I GCAGC 2 cut(s) 283, 313
LweI GCATC 2 cut(s) 191, 303
MaeI CTAG 3 cut(s) 302, 470, 768
MaeII ACGT 1 cut(s) 155
MaeIII GTNAC 2 cut(s) 61, 716
MalI GATC 2 cut(s) 214, 747
MboI GATC 2 cut(s) 212, 745
MboII GAAGA 5 cut(s) 127, 277, 390, 414, 441
MfeI CAATTG 2 cut(s) 721, 804
MflI RGATCY 1 cut(s) 212
MhlI GDGCHC 1 cut(s) 180
MlsI TGGCCA 2 cut(s) 224, 335
MluCI AATT 9 cut(s) 190, 274, 454, 671, 684, 701, 721, 782, 804
MluNI TGGCCA 2 cut(s) 224, 335
Mly113I GGCGCC 1 cut(s) 665
MlyI GAGTC 2 cut(s) 579, 793
MmeI TCCRAC 1 cut(s) 516
Mox20I TGGCCA 2 cut(s) 224, 335
MscI TGGCCA 2 cut(s) 224, 335
MseI TTAA 3 cut(s) 704, 764, 811
MslI CAYNNNNRTG 1 cut(s) 380
Msp20I TGGCCA 2 cut(s) 224, 335
MspI CCGG 3 cut(s) 71, 162, 757
MspR9I CCNGG 3 cut(s) 72, 656, 758
MunI CAATTG 2 cut(s) 721, 804
MvaI CCWGG 1 cut(s) 656
MwoI GCNNNNNNNGC 1 cut(s) 332
NarI GGCGCC 1 cut(s) 665
NciI CCSGG 2 cut(s) 72, 758
NcoI CCATGG 3 cut(s) 219, 230, 381
NdeII GATC 2 cut(s) 212, 745
NlaIII CATG 4 cut(s) 59, 223, 234, 385
NlaIV GGNNCC 5 cut(s) 76, 214, 611, 653, 666
NmeAIII GCCGAG 1 cut(s) 567
NmuCI GTSAC 2 cut(s) 61, 716
PciSI GCTCTTC 1 cut(s) 454
PcsI WCGNNNNNNNCGW 1 cut(s) 524
PflMI CCANNNNNTGG 2 cut(s) 97, 231
PfoI TCCNGGA 1 cut(s) 70
PinAI ACCGGT 1 cut(s) 161
PkrI GCNGC 2 cut(s) 298, 328
PleI GAGTC 2 cut(s) 579, 793
PluTI GGCGCC 1 cut(s) 668
PpsI GAGTC 2 cut(s) 579, 793
Psp6I CCWGG 1 cut(s) 654
PspGI CCWGG 1 cut(s) 654
PspN4I GGNNCC 5 cut(s) 76, 214, 611, 653, 666
PspPI GGNCC 1 cut(s) 651
PsrI GAACNNNNNNTAC 2 cut(s) 733, 765
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 1 cut(s) 212
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
RseI CAYNNNNRTG 1 cut(s) 380
SapI GCTCTTC 1 cut(s) 454
SaqAI TTAA 3 cut(s) 704, 764, 811
SatI GCNGC 2 cut(s) 297, 327
Sau3AI GATC 2 cut(s) 212, 745
Sau96I GGNCC 1 cut(s) 651
SchI GAGTC 2 cut(s) 579, 793
ScrFI CCNGG 3 cut(s) 72, 656, 758
SduI GDGCHC 1 cut(s) 180
SfaNI GCATC 2 cut(s) 191, 303
SfoI GGCGCC 1 cut(s) 666
SgrAI CRCCGGYG 1 cut(s) 161
SinI GGWCC 1 cut(s) 651
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 9 cut(s) 190, 274, 454, 671, 684, 701, 721, 782, 804
SspDI GGCGCC 1 cut(s) 664
SspMI CTAG 3 cut(s) 302, 470, 768
StyD4I CCNGG 3 cut(s) 70, 654, 756
StyI CCWWGG 5 cut(s) 96, 219, 230, 301, 381
TaaI ACNGT 3 cut(s) 67, 528, 651
TaiI ACGT 1 cut(s) 158
TaqI TCGA 3 cut(s) 188, 367, 518
TaqII GACCGA 1 cut(s) 271
TasI AATT 9 cut(s) 190, 274, 454, 671, 684, 701, 721, 782, 804
Tru1I TTAA 3 cut(s) 704, 764, 811
Tru9I TTAA 3 cut(s) 704, 764, 811
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 2 cut(s) 61, 716
TseI GCWGC 2 cut(s) 296, 326
Tsp45I GTSAC 2 cut(s) 61, 716
TspDTI ATGAA 4 cut(s) 34, 121, 267, 747
TspRI CASTG 1 cut(s) 382
Van91I CCANNNNNTGG 2 cut(s) 97, 231
VpaK11BI GGWCC 1 cut(s) 651
XapI RAATTY 4 cut(s) 190, 274, 671, 782
XmaJI CCTAGG 1 cut(s) 301
XspI CTAG 3 cut(s) 302, 470, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.