pycom01g03070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001044
Physical Location & Seq
Forward (+)
2455863 .. 2457427
1565 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03070.6

Sequence Viewer

Length: 1128 bp
ATGAATGTCGGGTGTGGAACTTACCTCATTAACTTGCTGCCGTGGGGGTCCTCTTTGGCCTACTCTTTTGTATTGTTGAGCGTTCAACGCTCGATTACATTTGACGTACAATTGGTAGGAGCCCCTCGTAGAAGTATTGTAGAAGTAACTCCTCCTTCATCTGATGTTGTGGACAAGAATCCACTTATCCTTTTACGTAGAAGGATGATTCGAGAAGTTGGGAAAAACTTCTCCAGAAATGCCCTCTTCATACTCTCCCATGATGTGACGGTTCCGGGAGCTAGTTCATACAACCAATCTTTGGCTTTGTCCATTAGAGAGAATGGAAAAGCCTTCATCTTCAAAATACTTCCATCGACGTTGACGGGAGTCATGCTTGAACACACCACTTCGAATTCTTTCAGGTGCTTGTTCGGATCCTCCATGGACAACCCATGGTATTTTGGAATGTGGTGGAGCAAACTTGACTTCAACTCGAACTCTTCGGTCTTACCTTGGCTCCTCGAGTGTTCGGTTGTCCATGGCCATGCCTTGTGTTTCTTCAAAAATCCTTGCCGTGCCCCCCTTCTTCCTCTTCTAGGACTTGTTCTTCAAGGGCAGGTTCTGGGCTTGGTGGATTTGATTCGTGTTGCTTCCTCTTTCGTCTCAAAGTCCTCTCGAAATCGCTGTCAAAGTCCAAGATATGCTCACGAATCGGTTGAGAACTCCGAGTCATACACTATAACAATCCAAGGGATTAGCAAAGTTGCTAATCCCCGGCAACGGCGCCAAAAATTTGATGCGAAATATATAAGTATAAGTAGGGATCGTTCTAAACCGGGGATTAGGAGGGATTACTCAAAACAGCACCAAAACACTCAAAACTGCCTTAAAAACACAATCTGGGCAGTTTTGAGCACCTACACTAATTTGGACGAATTTGGACGCTTTCTAACTAAATTGAACACTAAAGTAAAGGGGGATCTAGTTTTGACGAAATTAAACCAAAGTGCACAGATTGTAATAAAAACATATTGTAAATACGAATTTGATGAAAATATGGATGGAATGCTAGCTAGAAGGTTCTTCTCCACACATGTCACACTTGCATACAAGATGATTTTCAGTTGGTCTTTCGATGAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

376

Amino Acids

42.58

Weight (kDa)

9.68

Isoelectric Point (pI)

35.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 589
AccB1I GGYRCC 1 cut(s) 765
AccB7I CCANNNNNTGG 1 cut(s) 301
AclWI GGATC 4 cut(s) 411, 424, 813, 969
AcoI YGGCCR 1 cut(s) 523
AcsI RAATTY 4 cut(s) 394, 773, 917, 1025
AcyI GRCGYC 1 cut(s) 766
AfaI GTAC 1 cut(s) 108
AfiI CCNNNNNNNGG 3 cut(s) 301, 578, 762
AflIII ACRYGT 1 cut(s) 1075
AgsI TTSAA 7 cut(s) 86, 343, 380, 472, 544, 593, 943
AluBI AGCT 2 cut(s) 281, 1055
AluI AGCT 2 cut(s) 281, 1055
Alw21I GWGCWC 2 cut(s) 899, 994
Alw26I GTCTC 1 cut(s) 649
Alw44I GTGCAC 1 cut(s) 990
AlwI GGATC 4 cut(s) 411, 424, 813, 969
AlwNI CAGNNNCTG 1 cut(s) 604
Ama87I CYCGRG 1 cut(s) 503
AoxI GGCC 2 cut(s) 57, 523
ApaLI GTGCAC 1 cut(s) 990
ApeKI GCWGC 1 cut(s) 37
ApoI RAATTY 4 cut(s) 394, 773, 917, 1025
ArsI GACNNNNNNTTYG 2 cut(s) 652, 684
Asp700I GAANNNNTTC 2 cut(s) 227, 398
AspLEI GCGC 1 cut(s) 768
AspS9I GGNCC 1 cut(s) 48
AsuC2I CCSGG 3 cut(s) 276, 757, 819
AsuII TTCGAA 1 cut(s) 392
AsuNHI GCTAGC 1 cut(s) 1051
AvaI CYCGRG 1 cut(s) 503
AvaII GGWCC 1 cut(s) 48
BaeGI GKGCMC 2 cut(s) 562, 994
BalI TGGCCA 1 cut(s) 525
BamHI GGATCC 1 cut(s) 416
BanI GGYRCC 1 cut(s) 765
BanII GRGCYC 1 cut(s) 124
Bbv12I GWGCWC 2 cut(s) 899, 994
BbvI GCAGC 1 cut(s) 24
BccI CCATC 2 cut(s) 361, 1037
BceAI ACGGC 3 cut(s) 25, 540, 779
BcnI CCSGG 3 cut(s) 276, 757, 819
BcoDI GTCTC 1 cut(s) 649
BfaI CTAG 5 cut(s) 282, 578, 965, 1052, 1056
BfoI RGCGCY 1 cut(s) 769
BfuAI ACCTGC 1 cut(s) 589
BisI GCNGC 1 cut(s) 38
BlsI GCNGC 1 cut(s) 39
Bme1390I CCNGG 3 cut(s) 276, 757, 819
Bme18I GGWCC 1 cut(s) 48
BmeT110I CYCGRG 1 cut(s) 503
BmgT120I GGNCC 1 cut(s) 48
BmiI GGNNCC 6 cut(s) 49, 121, 273, 418, 500, 767
BmrFI CCNGG 3 cut(s) 276, 757, 819
BmsI GCATC 1 cut(s) 769
BmtI GCTAGC 1 cut(s) 1055
BoxI GACNNNNGTC 1 cut(s) 368
BpmI CTGGAG 1 cut(s) 217
Bpu14I TTCGAA 1 cut(s) 392
BpuMI CCSGG 3 cut(s) 276, 757, 819
BsaAI YACGTR 1 cut(s) 197
BsaHI GRCGYC 1 cut(s) 766
BsaJI CCNNGG 8 cut(s) 41, 423, 434, 494, 520, 730, 755, 818
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 578, 762
BseDI CCNNGG 8 cut(s) 41, 423, 434, 494, 520, 730, 755, 818
BseGI GGATG 2 cut(s) 210, 1048
BseLI CCNNNNNNNGG 3 cut(s) 301, 578, 762
BseRI GAGGAG 2 cut(s) 141, 491
BseSI GKGCMC 2 cut(s) 562, 994
BseXI GCAGC 1 cut(s) 24
BshFI GGCC 2 cut(s) 59, 525
BshNI GGYRCC 1 cut(s) 765
BsiHKAI GWGCWC 2 cut(s) 899, 994
BsiHKCI CYCGRG 1 cut(s) 503
BsiSI CCGG 3 cut(s) 275, 757, 818
BslI CCNNNNNNNGG 3 cut(s) 301, 578, 762
BsmAI GTCTC 1 cut(s) 649
BsmBI CGTCTC 1 cut(s) 649
BsmI GAATGC 1 cut(s) 1053
BsnI GGCC 2 cut(s) 59, 525
BsoBI CYCGRG 1 cut(s) 503
Bsp119I TTCGAA 1 cut(s) 392
Bsp1286I GDGCHC 4 cut(s) 124, 562, 899, 994
Bsp143I GATC 3 cut(s) 416, 805, 961
Bsp19I CCATGG 3 cut(s) 423, 434, 520
BspANI GGCC 2 cut(s) 59, 525
BspHI TCATGA 1 cut(s) 1124
BspLI GGNNCC 6 cut(s) 49, 121, 273, 418, 500, 767
BspMI ACCTGC 1 cut(s) 589
BspOI GCTAGC 1 cut(s) 1055
BspPI GGATC 4 cut(s) 411, 424, 813, 969
BspT104I TTCGAA 1 cut(s) 392
BspT107I GGYRCC 1 cut(s) 765
BssECI CCNNGG 8 cut(s) 41, 423, 434, 494, 520, 730, 755, 818
BssMI GATC 3 cut(s) 416, 805, 961
BssNI GRCGYC 1 cut(s) 766
BssT1I CCWWGG 5 cut(s) 423, 434, 494, 520, 730
Bst4CI ACNGT 1 cut(s) 271
Bst6I CTCTTC 3 cut(s) 251, 487, 579
BstACI GRCGYC 1 cut(s) 766
BstBAI YACGTR 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 392
BstC8I GCNNGC 1 cut(s) 1053
BstDSI CCRYGG 4 cut(s) 41, 423, 434, 520
BstENI CCTNNNNNAGG 1 cut(s) 576
BstF5I GGATG 2 cut(s) 210, 1048
BstH2I RGCGCY 1 cut(s) 769
BstHHI GCGC 1 cut(s) 768
BstKTI GATC 3 cut(s) 419, 808, 964
BstMAI GTCTC 1 cut(s) 649
BstMBI GATC 3 cut(s) 416, 805, 961
BstMWI GCNNNNNNNGC 1 cut(s) 87
BstNSI RCATGY 1 cut(s) 1079
BstPAI GACNNNNGTC 1 cut(s) 368
BstSCI CCNGG 3 cut(s) 274, 755, 817
BstSLI GKGCMC 2 cut(s) 562, 994
BstSNI TACGTA 1 cut(s) 197
BstV1I GCAGC 1 cut(s) 24
BstX2I RGATCY 2 cut(s) 416, 961
BstYI RGATCY 2 cut(s) 416, 961
BsuRI GGCC 2 cut(s) 59, 525
BtgI CCRYGG 4 cut(s) 41, 423, 434, 520
BtsCI GGATG 2 cut(s) 210, 1048
BveI ACCTGC 1 cut(s) 589
Cac8I GCNNGC 1 cut(s) 1053
CaiI CAGNNNCTG 1 cut(s) 604
CciI TCATGA 1 cut(s) 1124
CfoI GCGC 1 cut(s) 768
Cfr13I GGNCC 1 cut(s) 48
CseI GACGC 1 cut(s) 933
Csp6I GTAC 1 cut(s) 107
CviAII CATG 8 cut(s) 260, 373, 424, 435, 521, 527, 1076, 1125
CviJI RGCY 9 cut(s) 59, 122, 281, 305, 332, 499, 525, 609, 1055
CviKI_1 RGCY 9 cut(s) 59, 122, 281, 305, 332, 499, 525, 609, 1055
CviQI GTAC 1 cut(s) 107
DinI GGCGCC 1 cut(s) 767
DpnI GATC 3 cut(s) 418, 807, 963
DpnII GATC 3 cut(s) 416, 805, 961
EaeI YGGCCR 1 cut(s) 523
Eam1104I CTCTTC 3 cut(s) 251, 487, 579
EarI CTCTTC 3 cut(s) 251, 487, 579
Eco105I TACGTA 1 cut(s) 197
Eco130I CCWWGG 5 cut(s) 423, 434, 494, 520, 730
Eco24I GRGCYC 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 48
Eco88I CYCGRG 1 cut(s) 503
EcoNI CCTNNNNNAGG 1 cut(s) 576
EcoO109I RGGNCCY 1 cut(s) 48
EcoRI GAATTC 1 cut(s) 394
EcoT14I CCWWGG 5 cut(s) 423, 434, 494, 520, 730
EcoT38I GRGCYC 1 cut(s) 124
EgeI GGCGCC 1 cut(s) 767
EheI GGCGCC 1 cut(s) 767
ErhI CCWWGG 5 cut(s) 423, 434, 494, 520, 730
Esp3I CGTCTC 1 cut(s) 649
FaeI CATG 8 cut(s) 263, 376, 427, 438, 524, 530, 1079, 1128
FatI CATG 8 cut(s) 259, 372, 423, 434, 520, 526, 1075, 1124
Fnu4HI GCNGC 1 cut(s) 38
FokI GGATG 2 cut(s) 217, 1055
FriOI GRGCYC 1 cut(s) 124
Fsp4HI GCNGC 1 cut(s) 38
FspBI CTAG 5 cut(s) 282, 578, 965, 1052, 1056
GlaI GCGC 1 cut(s) 767
GluI GCNGC 1 cut(s) 38
GsuI CTGGAG 1 cut(s) 217
HaeII RGCGCY 1 cut(s) 769
HaeIII GGCC 2 cut(s) 59, 525
HapII CCGG 3 cut(s) 275, 757, 818
HgaI GACGC 1 cut(s) 933
HhaI GCGC 1 cut(s) 768
Hin1I GRCGYC 1 cut(s) 766
Hin1II CATG 8 cut(s) 263, 376, 427, 438, 524, 530, 1079, 1128
Hin6I GCGC 1 cut(s) 766
HinP1I GCGC 1 cut(s) 766
HincII GTYRAC 1 cut(s) 363
HindII GTYRAC 1 cut(s) 363
HinfI GANTC 7 cut(s) 178, 208, 369, 622, 692, 710, 1121
HpaII CCGG 3 cut(s) 275, 757, 818
Hpy166II GTNNAC 3 cut(s) 172, 363, 992
Hpy188I TCNGA 3 cut(s) 163, 416, 709
Hpy188III TCNNGA 5 cut(s) 212, 234, 657, 689, 1125
Hpy8I GTNNAC 3 cut(s) 172, 363, 992
Hpy99I CGWCG 1 cut(s) 361
HpyAV CCTTC 5 cut(s) 165, 195, 343, 575, 1053
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4IV ACGT 3 cut(s) 105, 196, 359
HpyCH4V TGCA 2 cut(s) 992, 1088
HpyF10VI GCNNNNNNNGC 1 cut(s) 87
HpySE526I ACGT 3 cut(s) 105, 196, 359
Hsp92I GRCGYC 1 cut(s) 766
Hsp92II CATG 8 cut(s) 263, 376, 427, 438, 524, 530, 1079, 1128
HspAI GCGC 1 cut(s) 766
KasI GGCGCC 1 cut(s) 765
Kzo9I GATC 3 cut(s) 416, 805, 961
LmnI GCTCC 4 cut(s) 119, 278, 456, 504
LpnPI CCDG 8 cut(s) 247, 288, 388, 584, 590, 770, 831, 868
Lsp1109I GCAGC 1 cut(s) 24
LweI GCATC 1 cut(s) 769
MaeI CTAG 5 cut(s) 282, 578, 965, 1052, 1056
MaeII ACGT 3 cut(s) 105, 196, 359
MaeIII GTNAC 3 cut(s) 145, 265, 1078
MalI GATC 3 cut(s) 418, 807, 963
MboI GATC 3 cut(s) 416, 805, 961
MboII GAAGA 8 cut(s) 238, 331, 474, 532, 560, 566, 581, 1057
MfeI CAATTG 1 cut(s) 110
MflI RGATCY 2 cut(s) 416, 961
MhlI GDGCHC 4 cut(s) 124, 562, 899, 994
MlsI TGGCCA 1 cut(s) 525
MluCI AATT 8 cut(s) 110, 394, 773, 907, 917, 938, 977, 1025
MluNI TGGCCA 1 cut(s) 525
Mly113I GGCGCC 1 cut(s) 766
MlyI GAGTC 2 cut(s) 378, 719
Mox20I TGGCCA 1 cut(s) 525
MroXI GAANNNNTTC 2 cut(s) 227, 398
MscI TGGCCA 1 cut(s) 525
MseI TTAA 3 cut(s) 30, 870, 980
MslI CAYNNNNRTG 1 cut(s) 525
Msp20I TGGCCA 1 cut(s) 525
MspI CCGG 3 cut(s) 275, 757, 818
MspR9I CCNGG 3 cut(s) 276, 757, 819
MunI CAATTG 1 cut(s) 110
Mva1269I GAATGC 1 cut(s) 1053
MwoI GCNNNNNNNGC 1 cut(s) 87
NarI GGCGCC 1 cut(s) 766
NciI CCSGG 3 cut(s) 276, 757, 819
NcoI CCATGG 3 cut(s) 423, 434, 520
NdeII GATC 3 cut(s) 416, 805, 961
NheI GCTAGC 1 cut(s) 1051
NlaIII CATG 8 cut(s) 263, 376, 427, 438, 524, 530, 1079, 1128
NlaIV GGNNCC 6 cut(s) 49, 121, 273, 418, 500, 767
NmuCI GTSAC 2 cut(s) 265, 1078
NspI RCATGY 1 cut(s) 1079
NspV TTCGAA 1 cut(s) 392
PaeR7I CTCGAG 1 cut(s) 503
PagI TCATGA 1 cut(s) 1124
PciI ACATGT 1 cut(s) 1075
PctI GAATGC 1 cut(s) 1053
PdmI GAANNNNTTC 2 cut(s) 227, 398
PfeI GAWTC 5 cut(s) 178, 208, 622, 692, 1121
PflMI CCANNNNNTGG 1 cut(s) 301
PfoI TCCNGGA 1 cut(s) 274
PkrI GCNGC 1 cut(s) 39
PleI GAGTC 2 cut(s) 377, 718
PluTI GGCGCC 1 cut(s) 769
PpsI GAGTC 2 cut(s) 377, 718
Ppu21I YACGTR 1 cut(s) 197
PpuMI RGGWCCY 1 cut(s) 48
PscI ACATGT 1 cut(s) 1075
PshAI GACNNNNGTC 1 cut(s) 368
Psp5II RGGWCCY 1 cut(s) 48
PspN4I GGNNCC 6 cut(s) 49, 121, 273, 418, 500, 767
PspPI GGNCC 1 cut(s) 48
PspPPI RGGWCCY 1 cut(s) 48
PspXI VCTCGAGB 1 cut(s) 503
PsrI GAACNNNNNNTAC 2 cut(s) 793, 825
PstNI CAGNNNCTG 1 cut(s) 604
PsuI RGATCY 2 cut(s) 416, 961
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
RseI CAYNNNNRTG 1 cut(s) 525
SaqAI TTAA 3 cut(s) 30, 870, 980
SatI GCNGC 1 cut(s) 38
Sau3AI GATC 3 cut(s) 416, 805, 961
Sau96I GGNCC 1 cut(s) 48
SchI GAGTC 2 cut(s) 378, 719
ScrFI CCNGG 3 cut(s) 276, 757, 819
SduI GDGCHC 4 cut(s) 124, 562, 899, 994
SfaNI GCATC 1 cut(s) 769
SfoI GGCGCC 1 cut(s) 767
Sfr274I CTCGAG 1 cut(s) 503
SfuI TTCGAA 1 cut(s) 392
SinI GGWCC 1 cut(s) 48
SlaI CTCGAG 1 cut(s) 503
SmiMI CAYNNNNRTG 1 cut(s) 525
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
SnaBI TACGTA 1 cut(s) 197
Sse9I AATT 8 cut(s) 110, 394, 773, 907, 917, 938, 977, 1025
SspDI GGCGCC 1 cut(s) 765
SspMI CTAG 5 cut(s) 282, 578, 965, 1052, 1056
StyD4I CCNGG 3 cut(s) 274, 755, 817
StyI CCWWGG 5 cut(s) 423, 434, 494, 520, 730
TaaI ACNGT 1 cut(s) 271
TaiI ACGT 3 cut(s) 108, 199, 362
TaqI TCGA 8 cut(s) 92, 211, 356, 392, 476, 504, 658, 1116
TaqII GACCGA 1 cut(s) 475
TasI AATT 8 cut(s) 110, 394, 773, 907, 917, 938, 977, 1025
TfiI GAWTC 5 cut(s) 178, 208, 622, 692, 1121
Tru1I TTAA 3 cut(s) 30, 870, 980
Tru9I TTAA 3 cut(s) 30, 870, 980
TseFI GTSAC 2 cut(s) 265, 1078
TseI GCWGC 1 cut(s) 37
Tsp45I GTSAC 2 cut(s) 265, 1078
TspDTI ATGAA 6 cut(s) 17, 147, 238, 276, 325, 1047
Van91I CCANNNNNTGG 1 cut(s) 301
VneI GTGCAC 1 cut(s) 990
VpaK11BI GGWCC 1 cut(s) 48
XagI CCTNNNNNAGG 1 cut(s) 576
XapI RAATTY 4 cut(s) 394, 773, 917, 1025
XceI RCATGY 1 cut(s) 1079
XhoI CTCGAG 1 cut(s) 503
XmnI GAANNNNTTC 2 cut(s) 227, 398
XspI CTAG 5 cut(s) 282, 578, 965, 1052, 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.