RLG00000027716
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000639
Physical Location & Seq
Forward (+)
14282845 .. 14289053
6209 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027716

Sequence Viewer

Length: 1335 bp
ATGAGCTGCGGCGACTTCAGAGTTCCACCGGGATACGGATTCCATCCCACCGACGAGAGGCTGCTTAGTGACTTGGAGGAAAAGAATCAGTGCAAGGACTCCGAGATCCCCACCATCATCCCTGATATCGAGATGTGCAAGCACGAGCCTCACGAGTTACCAGAGTCTTGGGACAGTCAGCGCAAGTGGTACTTCTTCACTCCGCGTGAATACAAGTACCCTAACAGCAGACGCTCCAACAGGACTACAGGGGAAGGGTTCTGGAAGATCACAGGCAAGGACCGCGACATCAAGGCCACTGGCTCCAAAGCTGTGATTGGGAGAAAGAGGACCTTAACCTTCTACAAACGTGGAGTGCCGAAATCCCAGAGGACTGGCTGGGTCATTCATGAGTATTATCTCATTGAGGCACATTCCGATCCTCCTAAGCAGATAGGGGACTTTGTTGTCTGTTGCTTGAAGTACAAGTCAGACAACTCTGATCATGACGAGGATGCTCCATTCGGCAATCGAGATAGCACTGGCTGCAGTATGGCCTCCAATGTTGAAAATCAAGCTGAAGAAAAGTGGGCAGCCATTGAGCAGGTTAATGTCGTTCCAATTCCCAATGGAAATGGTGATGAAGCTGAAACTGGTGGTGGTATGATTACAGAAGAAGAGGAATACCTGACTTTAAAGGAGCTGCAAAATGCTGTTCAGAATCCCATTGGCAATCCTAATGAATTCCAAGGCACGCAAACTGATCCTTGTCCTATAGATAAGAATGATCTTTCGACCTCTGTTGAAGGCCGACCTGATAAAAGCAATTTGTCTGATATGATTGAAGAGCTACTAGATGACCCAGAAAATCTGGATTCACTCTTTGATCCGCCTGCGCCACCGCAACTGCATCAGCTGCCAATGCATACCACCAATGATGAATGTCGTAAGAGGGCATATCCATTTGAGGACAATGAGCCTTCTCTTGCAAAGAAGAATAGTATTTCAACCAATGACGATGACGAATTAGTTAGCAAGACCGCATCTAGTTCGGAAAATCAAGCTGCAATTATGATTCAAGAGGTTACAAAGATACAGACAGCGGCCTGCGTGGCTGAGCGGGGGAAAAGAAGCCTTGTGATCAGTGAGTGGGATCTGGCGGGGTGTTGGTCGCGGTCGGCCGCCGGAGGCTTTGGTGACGACGATGGTAGGTTGGCGGTGGGGATTTTCTCGGGCTTGCCTATTTGCCTGGGGTCGGGCCTGGTGGTCTGGGCCGGTGTCATATCGTTTCAGTATCTGTTGGGCCTATTTGAAGGGGTTTGCCCTAAATGGGCTTGGGCCTCTAAATGGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

445

Amino Acids

49.54

Weight (kDa)

4.78

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 9 - 133 1.7e-34 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000639)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020
rosa_chinensis RchiOBHm_Chr1g0324821 RchiOBHm_Chr1g0333801 RchiOBHm_Chr1g0361601 RchiOBHm_Chr1g0361611 RchiOBHm_Chr1g0361661
rosa_laevigata RLG00000027711 RLG00000027712 RLG00000027714 RLG00000027715 RLG00000027716 RLG00000029530 RLG00000029531 RLG00000030172
rosa_multiflora Rmu_co8302323.1_g000001 Rmu_co8459497.1_g000001 Rmu_sc0003426.1_g000013 Rmu_sc0003693.1_g000002 Rmu_sc0003693.1_g000003 Rmu_sc0003704.1_g000001 Rmu_sc0003704.1_g000002 Rmu_sc0006101.1_g000001 Rmu_sc0006101.1_g000010 Rmu_sc0006417.1_g000001 Rmu_sc0006417.1_g000002 Rmu_sc0006417.1_g000005 Rmu_sc0007217.1_g000011 Rmu_sc0010981.1_g000006 Rmu_sc0011258.1_g000006 Rmu_sc0017253.1_g000003 Rmu_sc0017253.1_g000004 Rmu_sc0017750.1_g000001 Rmu_sc0026316.1_g000001 Rmu_sc0038193.1_g000002 Rmu_sc0041549.1_g000001
rosa_roxburghii Rroxscaffold_159G00433000 Rroxscaffold_159G00433050 Rroxscaffold_159G00433080 Rroxscaffold_4G00294830 Rroxscaffold_4G00294840 Rroxscaffold_4G00294900 Rroxscaffold_4G00294910 Rroxscaffold_4G00294920 Rroxscaffold_4G00317230 Rroxscaffold_4G00325170 Rroxscaffold_4G00325200 Rroxscaffold_4G00325280
rosa_rugosa Rorug01G0048000 Rorug01G0111600 Rorug01G0111700 Rorug01G0290200 Rorug01G0290300 Rorug01G0290300 Rorug01G0290400 Rorug01G0290600
rosa_samantha Rh1AG064300 Rh1AG064500 Rh1AG134000 Rh1AG134100 Rh1AG300500 Rh1AG300600
rosa_wichuraiana Rw1G005340 Rw1G005390 Rw1G005420 Rw1G011180 Rw1G011190 Rw1G026650 Rw1G026660 Rw1G026680 Rw1G026690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 446
Acc36I ACCTGC 1 cut(s) 574
AccBSI CCGCTC 1 cut(s) 1099
AccII CGCG 3 cut(s) 205, 285, 1153
AclWI GGATC 5 cut(s) 100, 413, 737, 860, 1140
AcoI YGGCCR 1 cut(s) 1158
AcsI RAATTY 1 cut(s) 722
AcuI CTGAAG 1 cut(s) 579
AfaI GTAC 3 cut(s) 191, 218, 464
AfiI CCNNNNNNNGG 5 cut(s) 35, 57, 1234, 1309, 1326
AgsI TTSAA 7 cut(s) 460, 548, 785, 824, 987, 1058, 1292
AjnI CCWGG 2 cut(s) 1227, 1239
AluBI AGCT 8 cut(s) 6, 311, 557, 626, 682, 829, 895, 1043
AluI AGCT 8 cut(s) 6, 311, 557, 626, 682, 829, 895, 1043
AlwI GGATC 5 cut(s) 100, 413, 737, 860, 1140
AlwNI CAGNNNCTG 1 cut(s) 1276
Ama87I CYCGRG 1 cut(s) 1210
AoxI GGCC 9 cut(s) 294, 534, 787, 1083, 1158, 1237, 1251, 1282, 1317
ApeKI GCWGC 7 cut(s) 6, 61, 525, 572, 682, 895, 1043
ApoI RAATTY 1 cut(s) 722
AspLEI GCGC 2 cut(s) 183, 877
AspS9I GGNCC 7 cut(s) 280, 330, 1237, 1251, 1282, 1317, 1329
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 2 cut(s) 629, 1187
AvaI CYCGRG 1 cut(s) 1210
AvaII GGWCC 3 cut(s) 280, 330, 1329
BauI CACGAG 2 cut(s) 143, 152
BbvI GCAGC 6 cut(s) 48, 512, 584, 669, 882, 1030
BccI CCATC 3 cut(s) 51, 122, 1178
BcgI CGANNNNNNTGC 2 cut(s) 1011, 1045
BciT130I CCWGG 2 cut(s) 1229, 1241
BciVI GTATCC 1 cut(s) 26
BclI TGATCA 2 cut(s) 481, 1119
BcnI CCSGG 1 cut(s) 30
BfaI CTAG 2 cut(s) 833, 1026
BfmI CTRYAG 3 cut(s) 246, 526, 753
BfuAI ACCTGC 1 cut(s) 574
BfuI GTATCC 1 cut(s) 26
BglI GCCNNNNNGGC 1 cut(s) 1091
BlpI GCTNAGC 1 cut(s) 1095
Bme1390I CCNGG 3 cut(s) 30, 1229, 1241
Bme18I GGWCC 3 cut(s) 280, 330, 1329
BmeT110I CYCGRG 1 cut(s) 1210
BmgT120I GGNCC 7 cut(s) 280, 330, 1237, 1251, 1282, 1317, 1329
BmiI GGNNCC 1 cut(s) 304
BmrFI CCNGG 3 cut(s) 30, 1229, 1241
BmsI GCATC 3 cut(s) 484, 898, 1031
Bpu10I CCTNAGC 1 cut(s) 426
Bpu1102I GCTNAGC 1 cut(s) 1095
BpuMI CCSGG 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 727, 1228
Bsc4I CCNNNNNNNGG 5 cut(s) 35, 57, 1234, 1309, 1326
Bse118I RCCGGY 1 cut(s) 1253
Bse1I ACTGG 4 cut(s) 304, 379, 526, 637
BseBI CCWGG 2 cut(s) 1229, 1241
BseDI CCNNGG 2 cut(s) 727, 1228
BseGI GGATG 3 cut(s) 43, 117, 499
BseLI CCNNNNNNNGG 5 cut(s) 35, 57, 1234, 1309, 1326
BseMII CTCAG 1 cut(s) 1086
BseNI ACTGG 4 cut(s) 304, 379, 526, 637
BseX3I CGGCCG 1 cut(s) 1158
BseXI GCAGC 6 cut(s) 48, 512, 584, 669, 882, 1030
BseYI CCCAGC 1 cut(s) 378
Bsh1236I CGCG 3 cut(s) 205, 285, 1153
Bsh1285I CGRYCG 2 cut(s) 1157, 1161
BshFI GGCC 9 cut(s) 296, 536, 789, 1085, 1160, 1239, 1253, 1284, 1319
BsiEI CGRYCG 2 cut(s) 1157, 1161
BsiHKCI CYCGRG 1 cut(s) 1210
BsiSI CCGG 3 cut(s) 29, 1164, 1254
BslFI GGGAC 2 cut(s) 185, 452
BslI CCNNNNNNNGG 5 cut(s) 35, 57, 1234, 1309, 1326
BsmFI GGGAC 2 cut(s) 185, 452
BsnI GGCC 9 cut(s) 296, 536, 789, 1085, 1160, 1239, 1253, 1284, 1319
BsoBI CYCGRG 1 cut(s) 1210
Bsp143I GATC 9 cut(s) 105, 267, 418, 481, 742, 766, 865, 1119, 1132
Bsp1720I GCTNAGC 1 cut(s) 1095
BspANI GGCC 9 cut(s) 296, 536, 789, 1085, 1160, 1239, 1253, 1284, 1319
BspCNI CTCAG 1 cut(s) 1087
BspFNI CGCG 3 cut(s) 205, 285, 1153
BspHI TCATGA 2 cut(s) 388, 484
BspLI GGNNCC 1 cut(s) 304
BspMAI CTGCAG 1 cut(s) 530
BspMI ACCTGC 1 cut(s) 574
BspPI GGATC 5 cut(s) 100, 413, 737, 860, 1140
BspQI GCTCTTC 1 cut(s) 819
BsrBI CCGCTC 1 cut(s) 1099
BsrFI RCCGGY 1 cut(s) 1253
BsrI ACTGG 4 cut(s) 304, 379, 526, 637
BssAI RCCGGY 1 cut(s) 1253
BssECI CCNNGG 2 cut(s) 727, 1228
BssMI GATC 9 cut(s) 105, 267, 418, 481, 742, 766, 865, 1119, 1132
BssSI CACGAG 2 cut(s) 143, 152
BssT1I CCWWGG 1 cut(s) 727
Bst2BI CACGAG 2 cut(s) 143, 152
Bst2UI CCWGG 2 cut(s) 1229, 1241
Bst4CI ACNGT 1 cut(s) 176
Bst6I CTCTTC 2 cut(s) 651, 819
BstAPI GCANNNNNTGC 2 cut(s) 525, 895
BstC8I GCNNGC 5 cut(s) 140, 734, 873, 1087, 1217
BstDEI CTNAG 3 cut(s) 65, 426, 1095
BstF5I GGATG 3 cut(s) 43, 117, 499
BstFNI CGCG 3 cut(s) 205, 285, 1153
BstHHI GCGC 2 cut(s) 183, 877
BstKTI GATC 9 cut(s) 108, 270, 421, 484, 745, 769, 868, 1122, 1135
BstMBI GATC 9 cut(s) 105, 267, 418, 481, 742, 766, 865, 1119, 1132
BstMCI CGRYCG 2 cut(s) 1157, 1161
BstMWI GCNNNNNNNGC 5 cut(s) 282, 525, 895, 901, 1091
BstNI CCWGG 2 cut(s) 1229, 1241
BstSCI CCNGG 3 cut(s) 28, 1227, 1239
BstSFI CTRYAG 3 cut(s) 246, 526, 753
BstUI CGCG 3 cut(s) 205, 285, 1153
BstV1I GCAGC 6 cut(s) 48, 512, 584, 669, 882, 1030
BstX2I RGATCY 2 cut(s) 105, 1132
BstXI CCANNNNNNTGG 2 cut(s) 168, 374
BstYI RGATCY 2 cut(s) 105, 1132
BstZI CGGCCG 1 cut(s) 1158
BsuI GTATCC 1 cut(s) 26
BsuRI GGCC 9 cut(s) 296, 536, 789, 1085, 1160, 1239, 1253, 1284, 1319
BtsCI GGATG 3 cut(s) 43, 117, 499
BtsIMutI CAGTG 4 cut(s) 95, 297, 519, 1129
BveI ACCTGC 1 cut(s) 574
Cac8I GCNNGC 5 cut(s) 140, 734, 873, 1087, 1217
CaiI CAGNNNCTG 1 cut(s) 1276
CciI TCATGA 2 cut(s) 388, 484
CfoI GCGC 2 cut(s) 183, 877
Cfr10I RCCGGY 1 cut(s) 1253
Cfr13I GGNCC 7 cut(s) 280, 330, 1237, 1251, 1282, 1317, 1329
CseI GACGC 1 cut(s) 240
Csp6I GTAC 3 cut(s) 190, 217, 463
CviAII CATG 2 cut(s) 389, 485
CviQI GTAC 3 cut(s) 190, 217, 463
DdeI CTNAG 3 cut(s) 65, 426, 1095
DpnI GATC 9 cut(s) 107, 269, 420, 483, 744, 768, 867, 1121, 1134
DpnII GATC 9 cut(s) 105, 267, 418, 481, 742, 766, 865, 1119, 1132
DraI TTTAAA 1 cut(s) 675
DrdI GACNNNNNNGTC 1 cut(s) 446
DseDI GACNNNNNNGTC 1 cut(s) 446
EaeI YGGCCR 1 cut(s) 1158
EagI CGGCCG 1 cut(s) 1158
Eam1104I CTCTTC 2 cut(s) 651, 819
EarI CTCTTC 2 cut(s) 651, 819
EciI GGCGGA 1 cut(s) 858
EclXI CGGCCG 1 cut(s) 1158
Eco130I CCWWGG 1 cut(s) 727
Eco32I GATATC 1 cut(s) 127
Eco47I GGWCC 3 cut(s) 280, 330, 1329
Eco52I CGGCCG 1 cut(s) 1158
Eco57I CTGAAG 1 cut(s) 579
Eco88I CYCGRG 1 cut(s) 1210
EcoO109I RGGNCCY 1 cut(s) 330
EcoRI GAATTC 1 cut(s) 722
EcoRII CCWGG 2 cut(s) 1227, 1239
EcoRV GATATC 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 727
EcoT22I ATGCAT 1 cut(s) 906
ErhI CCWWGG 1 cut(s) 727
FaeI CATG 2 cut(s) 392, 488
FalI AAGNNNNNCTT 4 cut(s) 176, 208, 317, 349
FaqI GGGAC 2 cut(s) 185, 452
FatI CATG 2 cut(s) 388, 484
FauI CCCGC 2 cut(s) 1092, 1132
FbaI TGATCA 2 cut(s) 481, 1119
FokI GGATG 3 cut(s) 30, 104, 506
FspBI CTAG 2 cut(s) 833, 1026
GlaI GCGC 2 cut(s) 182, 876
GsaI CCCAGC 1 cut(s) 382
HaeIII GGCC 9 cut(s) 296, 536, 789, 1085, 1160, 1239, 1253, 1284, 1319
HapII CCGG 3 cut(s) 29, 1164, 1254
HgaI GACGC 1 cut(s) 240
HhaI GCGC 2 cut(s) 183, 877
Hin1II CATG 2 cut(s) 392, 488
Hin6I GCGC 2 cut(s) 181, 875
HinP1I GCGC 2 cut(s) 181, 875
HinfI GANTC 7 cut(s) 39, 85, 98, 164, 700, 854, 1054
HpaII CCGG 3 cut(s) 29, 1164, 1254
HphI GGTGA 2 cut(s) 629, 1187
Hpy188I TCNGA 8 cut(s) 20, 103, 418, 472, 481, 699, 814, 1033
Hpy188III TCNNGA 8 cut(s) 130, 152, 262, 389, 485, 512, 851, 1058
Hpy99I CGWCG 2 cut(s) 56, 1184
HpyAV CCTTC 5 cut(s) 248, 349, 779, 969, 1286
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 8 cut(s) 93, 138, 528, 685, 889, 904, 968, 1046
HpyF10VI GCNNNNNNNGC 5 cut(s) 282, 525, 895, 901, 1091
HpyF3I CTNAG 3 cut(s) 65, 426, 1095
HpySE526I ACGT 1 cut(s) 349
Hsp92II CATG 2 cut(s) 392, 488
HspAI GCGC 2 cut(s) 181, 875
Ksp22I TGATCA 2 cut(s) 481, 1119
Kzo9I GATC 9 cut(s) 105, 267, 418, 481, 742, 766, 865, 1119, 1132
LguI GCTCTTC 1 cut(s) 819
LmnI GCTCC 4 cut(s) 239, 308, 502, 679
Lsp1109I GCAGC 6 cut(s) 48, 512, 584, 669, 882, 1030
LweI GCATC 3 cut(s) 484, 898, 1031
MaeI CTAG 2 cut(s) 833, 1026
MaeII ACGT 1 cut(s) 349
MaeIII GTNAC 4 cut(s) 68, 156, 1063, 1175
MalI GATC 9 cut(s) 107, 269, 420, 483, 744, 768, 867, 1121, 1134
MbiI CCGCTC 1 cut(s) 1099
MboI GATC 9 cut(s) 105, 267, 418, 481, 742, 766, 865, 1119, 1132
MboII GAAGA 7 cut(s) 187, 277, 572, 665, 668, 836, 985
MflI RGATCY 2 cut(s) 105, 1132
MluCI AATT 5 cut(s) 600, 722, 805, 1004, 1047
MlyI GAGTC 2 cut(s) 92, 173
MmeI TCCRAC 1 cut(s) 261
Mph1103I ATGCAT 1 cut(s) 906
MseI TTAA 3 cut(s) 335, 588, 674
MspA1I CMGCKG 2 cut(s) 895, 1082
MspI CCGG 3 cut(s) 29, 1164, 1254
MspR9I CCNGG 3 cut(s) 30, 1229, 1241
MvaI CCWGG 2 cut(s) 1229, 1241
MvnI CGCG 3 cut(s) 205, 285, 1153
MwoI GCNNNNNNNGC 5 cut(s) 282, 525, 895, 901, 1091
NciI CCSGG 1 cut(s) 30
NdeII GATC 9 cut(s) 105, 267, 418, 481, 742, 766, 865, 1119, 1132
NlaIII CATG 2 cut(s) 392, 488
NlaIV GGNNCC 1 cut(s) 304
NmuCI GTSAC 2 cut(s) 68, 1175
NsiI ATGCAT 1 cut(s) 906
PagI TCATGA 2 cut(s) 388, 484
PciSI GCTCTTC 1 cut(s) 819
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PfeI GAWTC 5 cut(s) 39, 85, 700, 854, 1054
PleI GAGTC 2 cut(s) 92, 172
PpsI GAGTC 2 cut(s) 92, 172
PpuMI RGGWCCY 1 cut(s) 330
Psp5II RGGWCCY 1 cut(s) 330
Psp6I CCWGG 2 cut(s) 1227, 1239
PspFI CCCAGC 1 cut(s) 378
PspGI CCWGG 2 cut(s) 1227, 1239
PspN4I GGNNCC 1 cut(s) 304
PspPI GGNCC 7 cut(s) 280, 330, 1237, 1251, 1282, 1317, 1329
PspPPI RGGWCCY 1 cut(s) 330
PstI CTGCAG 1 cut(s) 530
PstNI CAGNNNCTG 1 cut(s) 1276
PsuI RGATCY 2 cut(s) 105, 1132
PvuII CAGCTG 1 cut(s) 895
RsaI GTAC 3 cut(s) 191, 218, 464
RsaNI GTAC 3 cut(s) 190, 217, 463
SapI GCTCTTC 1 cut(s) 819
SaqAI TTAA 3 cut(s) 335, 588, 674
Sau3AI GATC 9 cut(s) 105, 267, 418, 481, 742, 766, 865, 1119, 1132
Sau96I GGNCC 7 cut(s) 280, 330, 1237, 1251, 1282, 1317, 1329
SchI GAGTC 2 cut(s) 92, 173
ScrFI CCNGG 3 cut(s) 30, 1229, 1241
SfaNI GCATC 3 cut(s) 484, 898, 1031
SfcI CTRYAG 3 cut(s) 246, 526, 753
SinI GGWCC 3 cut(s) 280, 330, 1329
Sse9I AATT 5 cut(s) 600, 722, 805, 1004, 1047
SspMI CTAG 2 cut(s) 833, 1026
StyD4I CCNGG 3 cut(s) 28, 1227, 1239
StyI CCWWGG 1 cut(s) 727
TaaI ACNGT 1 cut(s) 176
TaiI ACGT 1 cut(s) 352
TaqI TCGA 3 cut(s) 129, 511, 773
TasI AATT 5 cut(s) 600, 722, 805, 1004, 1047
TatI WGTACW 1 cut(s) 462
TauI GCSGC 3 cut(s) 12, 1085, 1163
TfiI GAWTC 5 cut(s) 39, 85, 700, 854, 1054
Tru1I TTAA 3 cut(s) 335, 588, 674
Tru9I TTAA 3 cut(s) 335, 588, 674
TscAI CASTG 4 cut(s) 95, 304, 526, 1129
TseFI GTSAC 2 cut(s) 68, 1175
TseI GCWGC 7 cut(s) 6, 61, 525, 572, 682, 895, 1043
Tsp45I GTSAC 2 cut(s) 68, 1175
TspDTI ATGAA 4 cut(s) 377, 636, 735, 933
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 4 cut(s) 95, 304, 526, 1129
VpaK11BI GGWCC 3 cut(s) 280, 330, 1329
XapI RAATTY 1 cut(s) 722
XspI CTAG 2 cut(s) 833, 1026
Zsp2I ATGCAT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.