Rorug01G0290200
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000639
Physical Location & Seq
Forward (+)
40613171 .. 40618838
5668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0290200.1

Sequence Viewer

Length: 4437 bp
ATGGATTGTCTTTGTGCAATTGGTAACGGGGTTGTTGGAAAAATAGCCGAGTACACGGTTGAACCCTTTGGACGTCTAGTGGGACGCCAAGTGGGTTATCTAATCCACTACAACAAGAATCTTCAAACTCTAATGACTCAAGTGAAGAATTTGAGCGTCGCCAGAGATAGAGTAAAGCAACAGGTTGATGAAGCTGATAGAAGAGGTGAGAAAATCCATAAGGATGTCCAAAAATGGCTCGAAGAAGTGGCTCAGATCATGAAAGACACAGAAGAATTCTTGAAGGGAAAATGCCAAGCAACTGGAGAGCCAAACCAAGTCTTGAAGGATGAAGGCCAAGCAAATGAAGAGCCAAACGAAGTCTTGAAAGATGAACGTCAAGGAAAGCTGAAGCGTCTCCATCAATTTTGTCCTAATCCGAGACTTCGTTATCAACTAAGCAGAAAGTCAACAAAGTTGGTGGAGACTGTGGATGAACTCTATCAAAGAAAAGACTTCTCTATTGTTGCATACAGTGGTCGCGCACAAGAGGTATGTCTCGCATCTACTGAAGACTATGAGGCATTTGATTCAAGGATCTCAACTTTGAATAAAATCATGGATGAATTGAGAAGTCCCAGTGCCGACATGATTCTAGTGTATGGTATTGGGGGTGTGGGTAAAACCACACTAGTGGAAGAACTTCTCAGGCAAGTTACGAAAGATAAGTTGTTTGATGATGCAGTCATGGTAAGAGATGTGAAAATTCCAAACTTGGAAACAATTCAAAAAGAAATTGCTGAAAAGTTAGGTCTTGGAGTTTTGTCAGACGAGACTATCGCCGGAAGAGCCAATAAAATATGTGATAGAATAAAAGACAAGAAGACTCTTGTGATTTTGGATGATGTCTGGGAAGAAATTGACTTGAAAACTCTCGGGCTTCCCCATATACCTACTTGTAAAATACTGCTGACCTCTAGAACTCGAAAGGTATTAACTTCTGGTAAGATTAAGCAGAAAGTATTTCTGCTTGGAGTGTTGGGAGAAAATGAAACTTGGCTTTTATTTGAGAAGAAAGCTGGCGATGTTACTAAAGATCCCACTATACTAAGCATAGCAACTCAAGTCGCCAAAAAATGTGGAGGTTTGCCTGTTTTGATTGTCACAGTTGCAAGAGCTTTAGAAAGTAGTTCTTTACCTATATGGAAAAATGCCCTGAGAAGTCTTGACAAGTTTGATGAGAAAGAAGAACTGACAGAAAAAGCTTACATGGGTCTTGAATGGAGTTACGCTCAATTGAATGAGCAGCTTAAGCCATTATTCCTGTTGTGTGGTATTACTATACAAGGGAACTCTATTTCTTTGACGAACTTGTTGAAATATAGTATGGGTTTGGGTTTTTTTAAAGACCATACAGTGGAAGAAGCTCGAAATGCATTGTATACACAGGTTGATAAACTTAAGAGTTCTTGTCTATTGCTAGACGGAGATGATAATAATACATGTGTCAGAATGCACGATCTTGTACATGATGTTGCTATCCGGATTGCGACCAGAGATCAACACGTCCTAGTAAAAGATGGAGTTGATCAGTTGGAAGAATGGCTAGATCAAGAATCGTTTAAAAAGTACAGTAAGATCTCCTGGTCCTGTTGCAATATCCCCAGTCTTCCTGAAGTACCTTGGAAATGTCCTGAGCTGGAAATGTTTCGTTTGCATGGTAATGGTGAAAAAAATGATCACCATTTGAAAATTCCAAGCAATTTTTTTGAAGAAATGCCAAAACTCAAGGTGTTGGATATTTGCAATCTGCCTCTTATGTTACTACCTCCATCTCTTGCATTGCTAAAAACCAATCTTCAAACATTGTGTTTAGATGGATGCACGTTGGAAGACATAGCTCTAGTTGGAGAGCTAAGCAACTTGGAAACTCTTAGCTTTGCTAACTCCAAGTTCAAAGAGTTGCCTAAAGAAATAGCGAAATTGACTCATCTGCGGTTGTTGGACTTGTCGGATTGCCCTGAGCTTGAAGTGATTTCACCTAATGTCATATCAAGTTTGACAAGGCTCGAAGACTTGAGAATGAAAAACAGTTTTGAAAAATGGGAGGCTGAGGAAGACATCAACAAAAGAAGGAATGCTAGTCTTTTAGAGTTGAAGGACTTATCGCAGTTAACTGCATTAGAAATATATATTCCAGATGCTGATATTCTTCCACCAAACTTGTTCTCCAATAAATTGGAGAGATACCATGTATATATTGGTGGTGTGTGGTCCAAAGATATGGCTGAAACGACCTTCAATACACTAAAGCTCAGGCTCACTCCCAGCAATCAATCGGATCAAGGTCTAAGAAGGCTGATGATGAAATCTGAAGATTTGGCCTTGGATGTGAGGGAAGGTATTAATAATAGTTTGTATCAATTAGACAACGAAGGTTTTCTGCACCTGAAACGCCTCCTACTCCAAAACAATGTTGATCTTTCATATATCAATGCCAAGAAGGTTGTGTTCCCCAACTTAACAACCTTGAGCATACAAAAATGTGATCATTTGAGATTCTTGTTCTCATGTTCTATGGCTAAAAGTTTCGTACAGCTCAAACATCTTGAGATATGCTACTGCTACAGCATGGAAGAGGTAGTATCATCAACAACAGATTACAATGAAGAAAATATGAATAACATGTTCTGTAAGCTTGAAACTCTAGAACTGGAAGAACTTCCATGCCTCGGTAGAATCTGCACAGGAACTCGTATTGAATTTCCGTCCTTGAAGAAGTTGGTTATAAGGTATTGTATAAAATTGAAGGCATTCATCTGTGACCCTATGAGTGATAAAAGTACAAGAATCTGCAAAGAAATTGAAGAGAGGGAGTCAGAGGAGAGCCCAAGTGTTGAACATGAGAGTGTTGCAGAACGCTGCCTTTTTAACGACAAGGTAACATTTCCTAGCTTGGAGGAATTGATAATATATCGGTTAGAGGAGTTGCAGACAATATGGCACAATAACCAACTTGCTGATCAAGACTCTTTTTGCCGACTCAAACAAGTTAACGTTCGTTTTTGCGAGAGTCTGATAAGTATATTTCCGCCTAGTATCATGGGGAGATTGAATGCTCTGGAAAGATTAGACATCGATACTTGCAACTCACTAAAAGTGGTATTTGAAGTCGGAGGAATTAATGTTGAAGAGACACGTGTCAACAGATCAACCCCTTATGATCAGTGGAAACCTTTGTACTGTCAAAATCTTGAATCCTTGAGGATATGGGGTGCTGACAGTTTGAAAAATATATTCCCAGCCTCTTTGGCCAGAGGACTTCAGCAGCTACGATATCTGAACGTGAAGTCTTGTCGTGATCTGCTGGAAATAGTTGGTGAGGAAGAGGGAGGAGTAGAATTACCAACGCCTCCTCAGTTTGTTTTCCCAAAGGCAACATATGTGTCATTTTATGAGCTGCCCCAACTGAAGAGTTTTTATCCGGGAAAGCATACTTCCACGTGGCCATTACTCAAAGAATTGACAGTAAGATTAAAAAGAGGAATTAATGAAGTAAAAATTTCAATCTTTCAGGATAAACATGAGTCGGGCCCAGTAAAGTCTATCTTTCCAATTGACAAGGATTCATTCCCCAACTTGGAAATTCTGTCCGTGGACAACGTGGATATTTGGTACGGTACATCCCCAGTACCAACAGAGTCCTTCAGCAACCTAAAATGTGTTGCATTTAGTAATTGGGCACATTTCTCCCAGCAAGCTACGTCACTCTTCCTTCAGAAATTACACAATATTGAAAGAATTGAATTGAATTGGGCTCCTCCCGAAGAAATATTTGTCAGTGAAGGAAACAGTAGTTGTGAGGAAATACATGCAGTTGGGACCCTACCAAGCATACGAATTTTAGAGTTTTACTATTGCAAAATGTTGCATCTAGGGAAGGAGAACTCCGGATCAGGAGGCCCAATTTTTCCAAACTTGGAAGTTCTAGAGGTGTCGCGACATTCTCCCGGATTAAAGAATTTGGAGTCATCAGCGATTTCCTTCCGGAATTTAACAACTTTGAAAGTAGTTGCTTGTCCGGGCTTGGAACATTTAATAACTTATTCAGTGGCTAAAAGCTTAAAGCAACTGACAACAATGGAAGTGAATTACTGTCCAAGAATTGCAGTCATAGTGGCAACAGACGGTGATGATGCAGCAGAAGAGATTACTTTCAGCCGATTGCAAGTTTTAACCCTTAATGGACTGCCAAAAATGCAAGGCTTTTGCTCCGGAAGTTGCATTGTGAATCTCCCATCCTTGACTACTTTATCCATGAGCAACCGCCTTAAATTGAAAATTTTCCAAGAAAAGCTAAAGATAACATTCGAGTACCCTGACACGGAGGAGGAGGAGGAAGACAATGTGGATGAGGAAATAGGCGTGCCGAGTGCTGAAACAAATATTGCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

1478

Amino Acids

167.87

Weight (kDa)

5.43

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_16 PF13191 192 - 238 1.3e-06 AAA ATPase domain
NB-ARC PF00931 195 - 355 4.5e-27 NB-ARC domain
LRR_14 PF23598 556 - 907 2.3e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 827 - 931 6e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 974 - 1043 4.5e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 980 - 1060 2.1e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1073 - 1113 1.3e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1334 - 1437 6.1e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000639)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020
rosa_chinensis RchiOBHm_Chr1g0324821 RchiOBHm_Chr1g0333801 RchiOBHm_Chr1g0361601 RchiOBHm_Chr1g0361611 RchiOBHm_Chr1g0361661
rosa_laevigata RLG00000027711 RLG00000027712 RLG00000027714 RLG00000027715 RLG00000027716 RLG00000029530 RLG00000029531 RLG00000030172
rosa_multiflora Rmu_co8302323.1_g000001 Rmu_co8459497.1_g000001 Rmu_sc0003426.1_g000013 Rmu_sc0003693.1_g000002 Rmu_sc0003693.1_g000003 Rmu_sc0003704.1_g000001 Rmu_sc0003704.1_g000002 Rmu_sc0006101.1_g000001 Rmu_sc0006101.1_g000010 Rmu_sc0006417.1_g000001 Rmu_sc0006417.1_g000002 Rmu_sc0006417.1_g000005 Rmu_sc0007217.1_g000011 Rmu_sc0010981.1_g000006 Rmu_sc0011258.1_g000006 Rmu_sc0017253.1_g000003 Rmu_sc0017253.1_g000004 Rmu_sc0017750.1_g000001 Rmu_sc0026316.1_g000001 Rmu_sc0038193.1_g000002 Rmu_sc0041549.1_g000001
rosa_roxburghii Rroxscaffold_159G00433000 Rroxscaffold_159G00433050 Rroxscaffold_159G00433080 Rroxscaffold_4G00294830 Rroxscaffold_4G00294840 Rroxscaffold_4G00294900 Rroxscaffold_4G00294910 Rroxscaffold_4G00294920 Rroxscaffold_4G00317230 Rroxscaffold_4G00325170 Rroxscaffold_4G00325200 Rroxscaffold_4G00325280
rosa_rugosa Rorug01G0048000 Rorug01G0111600 Rorug01G0111700 Rorug01G0290200 Rorug01G0290300 Rorug01G0290300 Rorug01G0290400 Rorug01G0290600
rosa_samantha Rh1AG064300 Rh1AG064500 Rh1AG134000 Rh1AG134100 Rh1AG300500 Rh1AG300600
rosa_wichuraiana Rw1G005340 Rw1G005390 Rw1G005420 Rw1G011180 Rw1G011190 Rw1G026650 Rw1G026660 Rw1G026680 Rw1G026690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2768
AatII GACGTC 1 cut(s) 76
AccB7I CCANNNNNTGG 1 cut(s) 1396
AccI GTMKAC 1 cut(s) 1421
AccII CGCG 2 cut(s) 522, 3979
AccIII TCCGGA 4 cut(s) 1521, 3929, 4026, 4253
AciI CCGC 3 cut(s) 1975, 3071, 4306
AclI AACGTT 1 cut(s) 3036
AclWI GGATC 4 cut(s) 584, 1070, 2328, 3940
AcoI YGGCCR 2 cut(s) 3291, 3485
AcuI CTGAAG 8 cut(s) 410, 570, 1674, 2373, 3287, 3470, 3670, 3740
AcvI CACGTG 2 cut(s) 3179, 3483
AcyI GRCGYC 2 cut(s) 73, 85
AfiI CCNNNNNNNGG 4 cut(s) 1396, 2711, 3619, 4363
AflII CTTAAG 2 cut(s) 1289, 1439
AflIII ACRYGT 5 cut(s) 1481, 1543, 2664, 3176, 3178
AhlI ACTAGT 1 cut(s) 670
AjiI CACGTC 1 cut(s) 1546
AjnI CCWGG 1 cut(s) 1622
AleI CACNNNNGTG 1 cut(s) 671
Alw26I GTCTC 6 cut(s) 401, 415, 458, 542, 806, 3167
AlwI GGATC 4 cut(s) 584, 1070, 2328, 3940
AlwNI CAGNNNCTG 1 cut(s) 2183
Ama87I CYCGRG 1 cut(s) 914
Aor13HI TCCGGA 4 cut(s) 1521, 3929, 4026, 4253
AoxI GGCC 6 cut(s) 334, 2361, 3291, 3485, 3571, 3940
ApaI GGGCCC 1 cut(s) 3575
ApeKI GCWGC 5 cut(s) 1285, 2901, 3307, 3439, 4178
AseI ATTAAT 3 cut(s) 2385, 3162, 3528
Asp700I GAANNNNTTC 7 cut(s) 681, 762, 1686, 2418, 2700, 2792, 4322
AspLEI GCGC 1 cut(s) 524
AspS9I GGNCC 6 cut(s) 1626, 2253, 3571, 3572, 3861, 3941
AsuC2I CCSGG 3 cut(s) 3465, 3990, 4062
AsuHPI GGTGA 6 cut(s) 218, 1712, 1718, 2010, 3371, 4181
AvaI CYCGRG 1 cut(s) 914
AvaII GGWCC 3 cut(s) 1626, 2253, 3861
BaeGI GKGCMC 2 cut(s) 3575, 3724
BalI TGGCCA 2 cut(s) 3293, 3487
BanII GRGCYC 3 cut(s) 2870, 3575, 3799
BbrPI CACGTG 2 cut(s) 3179, 3483
BbsI GAAGAC 7 cut(s) 558, 869, 1640, 1878, 2058, 2103, 4386
BbvCI CCTCAGC 1 cut(s) 2091
BbvI GCAGC 5 cut(s) 1297, 2888, 3319, 3426, 4190
BccI CCATC 5 cut(s) 408, 1553, 1819, 1850, 4285
BciT130I CCWGG 1 cut(s) 1624
BclI TGATCA 5 cut(s) 1567, 1717, 2527, 3001, 3202
BcnI CCSGG 3 cut(s) 3465, 3990, 4062
BcoDI GTCTC 6 cut(s) 401, 415, 458, 542, 806, 3167
BcuI ACTAGT 1 cut(s) 670
BfmI CTRYAG 1 cut(s) 2605
BfrI CTTAAG 2 cut(s) 1289, 1439
BglI GCCNNNNNGGC 1 cut(s) 3290
BglII AGATCT 1 cut(s) 1617
BisI GCNGC 5 cut(s) 1286, 2902, 3308, 3440, 4179
BlpI GCTNAGC 1 cut(s) 1895
BlsI GCNGC 5 cut(s) 1287, 2903, 3309, 3441, 4180
Bme1390I CCNGG 4 cut(s) 1624, 3465, 3990, 4062
Bme18I GGWCC 3 cut(s) 1626, 2253, 3861
BmeT110I CYCGRG 1 cut(s) 914
BmgBI CACGTC 1 cut(s) 1546
BmgT120I GGNCC 6 cut(s) 1626, 2253, 3571, 3572, 3861, 3941
BmiI GGNNCC 4 cut(s) 3573, 3798, 3862, 3863
BmrFI CCNGG 4 cut(s) 1624, 3465, 3990, 4062
BmrI ACTGGG 4 cut(s) 612, 1638, 3569, 3662
BmsI GCATC 6 cut(s) 551, 709, 1850, 2170, 3919, 4165
BmuI ACTGGG 4 cut(s) 612, 1638, 3569, 3662
BoxI GACNNNNGTC 1 cut(s) 3179
BpiI GAAGAC 7 cut(s) 558, 869, 1640, 1878, 2058, 2103, 4386
BplI GAGNNNNNCTC 2 cut(s) 1255, 1287
BpmI CTGGAG 1 cut(s) 324
Bpu10I CCTNAGC 4 cut(s) 1674, 2001, 2091, 2294
Bpu1102I GCTNAGC 1 cut(s) 1895
BpuEI CTTGAG 7 cut(s) 123, 1086, 1751, 2077, 2530, 2609, 3262
BpuMI CCSGG 3 cut(s) 3465, 3990, 4062
Bsa29I ATCGAT 1 cut(s) 3117
BsaAI YACGTR 2 cut(s) 3179, 3483
BsaHI GRCGYC 2 cut(s) 73, 85
BsaJI CCNNGG 4 cut(s) 1661, 2364, 2710, 3633
BsaWI WCCGGW 4 cut(s) 1521, 3929, 4026, 4253
BsaXI ACNNNNNCTCC 2 cut(s) 2192, 2222
Bsc4I CCNNNNNNNGG 4 cut(s) 1396, 2711, 3619, 4363
Bse1I ACTGG 6 cut(s) 307, 618, 1644, 2697, 3575, 3668
Bse3DI GCAATG 1 cut(s) 1820
BseAI TCCGGA 4 cut(s) 1521, 3929, 4026, 4253
BseBI CCWGG 1 cut(s) 1624
BseCI ATCGAT 1 cut(s) 3117
BseDI CCNNGG 4 cut(s) 1661, 2364, 2710, 3633
BseLI CCNNNNNNNGG 4 cut(s) 1396, 2711, 3619, 4363
BseMI GCAATG 1 cut(s) 1820
BseMII CTCAG 8 cut(s) 266, 700, 1187, 1665, 1992, 2082, 2308, 3410
BseNI ACTGG 6 cut(s) 307, 618, 1644, 2697, 3575, 3668
BseRI GAGGAG 8 cut(s) 2876, 2978, 3384, 3387, 3789, 4382, 4385, 4388
BseSI GKGCMC 2 cut(s) 3575, 3724
BseXI GCAGC 5 cut(s) 1297, 2888, 3319, 3426, 4190
BseYI CCCAGC 3 cut(s) 2306, 3280, 3732
BsgI GTGCAG 2 cut(s) 2408, 2707
Bsh1236I CGCG 2 cut(s) 522, 3979
BshFI GGCC 6 cut(s) 336, 2363, 3293, 3487, 3573, 3942
BshVI ATCGAT 1 cut(s) 3117
BsiHKCI CYCGRG 1 cut(s) 914
BsiSI CCGG 8 cut(s) 822, 1522, 3464, 3930, 3990, 4027, 4061, 4254
BslFI GGGAC 3 cut(s) 96, 600, 3874
BslI CCNNNNNNNGG 4 cut(s) 1396, 2711, 3619, 4363
BsmAI GTCTC 6 cut(s) 401, 415, 458, 542, 806, 3167
BsmBI CGTCTC 1 cut(s) 401
BsmFI GGGAC 3 cut(s) 96, 600, 3874
BsmI GAATGC 4 cut(s) 1497, 2122, 2792, 3100
BsnI GGCC 6 cut(s) 336, 2363, 3293, 3487, 3573, 3942
BsoBI CYCGRG 1 cut(s) 914
Bsp120I GGGCCC 1 cut(s) 3571
Bsp1286I GDGCHC 4 cut(s) 2870, 3575, 3724, 3799
Bsp13I TCCGGA 4 cut(s) 1521, 3929, 4026, 4253
Bsp1407I TGTACA 1 cut(s) 1504
Bsp1720I GCTNAGC 1 cut(s) 1895
Bsp68I TCGCGA 1 cut(s) 3979
BspACI CCGC 3 cut(s) 1975, 3071, 4306
BspANI GGCC 6 cut(s) 336, 2363, 3293, 3487, 3573, 3942
BspCNI CTCAG 8 cut(s) 265, 699, 1188, 1666, 1993, 2083, 2307, 3409
BspDI ATCGAT 1 cut(s) 3117
BspEI TCCGGA 4 cut(s) 1521, 3929, 4026, 4253
BspFNI CGCG 2 cut(s) 522, 3979
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 4 cut(s) 3573, 3798, 3862, 3863
BspPI GGATC 4 cut(s) 584, 1070, 2328, 3940
BspQI GCTCTTC 2 cut(s) 342, 820
BspTI CTTAAG 2 cut(s) 1289, 1439
BsrDI GCAATG 1 cut(s) 1820
BsrGI TGTACA 1 cut(s) 1504
BsrI ACTGG 6 cut(s) 307, 618, 1644, 2697, 3575, 3668
BssECI CCNNGG 4 cut(s) 1661, 2364, 2710, 3633
BssNAI GTATAC 1 cut(s) 1422
BssNI GRCGYC 2 cut(s) 73, 85
BssT1I CCWWGG 2 cut(s) 1661, 2364
Bst1107I GTATAC 1 cut(s) 1422
Bst2UI CCWGG 1 cut(s) 1624
BstACI GRCGYC 2 cut(s) 73, 85
BstAFI CTTAAG 2 cut(s) 1289, 1439
BstAUI TGTACA 1 cut(s) 1504
BstBAI YACGTR 2 cut(s) 3179, 3483
BstC8I GCNNGC 4 cut(s) 1060, 3738, 4406, 4432
BstDSI CCRYGG 1 cut(s) 3633
BstFNI CGCG 2 cut(s) 522, 3979
BstHHI GCGC 1 cut(s) 524
BstMAI GTCTC 6 cut(s) 401, 415, 458, 542, 806, 3167
BstMWI GCNNNNNNNGC 5 cut(s) 827, 1291, 1412, 3290, 4237
BstNI CCWGG 1 cut(s) 1624
BstNSI RCATGY 3 cut(s) 1485, 2668, 3854
BstPAI GACNNNNGTC 1 cut(s) 3179
BstSCI CCNGG 4 cut(s) 1622, 3463, 3988, 4060
BstSFI CTRYAG 1 cut(s) 2605
BstSLI GKGCMC 2 cut(s) 3575, 3724
BstUI CGCG 2 cut(s) 522, 3979
BstV1I GCAGC 5 cut(s) 1297, 2888, 3319, 3426, 4190
BstV2I GAAGAC 7 cut(s) 558, 869, 1640, 1878, 2058, 2103, 4386
BstX2I RGATCY 3 cut(s) 576, 1075, 1617
BstXI CCANNNNNNTGG 4 cut(s) 302, 673, 2218, 2263
BstYI RGATCY 3 cut(s) 576, 1075, 1617
BstZ17I GTATAC 1 cut(s) 1422
Bsu15I ATCGAT 1 cut(s) 3117
BsuRI GGCC 6 cut(s) 336, 2363, 3293, 3487, 3573, 3942
BsuTUI ATCGAT 1 cut(s) 3117
BtgI CCRYGG 1 cut(s) 3633
BtgZI GCGATG 1 cut(s) 1077
BtrI CACGTC 1 cut(s) 1546
BtsIMutI CAGTG 6 cut(s) 520, 625, 1401, 3212, 3826, 4095
BtuMI TCGCGA 1 cut(s) 3979
Cac8I GCNNGC 4 cut(s) 1060, 3738, 4406, 4432
CaiI CAGNNNCTG 1 cut(s) 2183
CciI TCATGA 1 cut(s) 258
CfoI GCGC 1 cut(s) 524
Cfr13I GGNCC 6 cut(s) 1626, 2253, 3571, 3572, 3861, 3941
ClaI ATCGAT 1 cut(s) 3117
CseI GACGC 3 cut(s) 93, 145, 383
CspCI CAANNNNNGTGG 6 cut(s) 441, 476, 1099, 1134, 2184, 2219
DraI TTTAAA 2 cut(s) 1384, 1603
EaeI YGGCCR 2 cut(s) 3291, 3485
EciI GGCGGA 1 cut(s) 3060
Eco130I CCWWGG 2 cut(s) 1661, 2364
Eco24I GRGCYC 3 cut(s) 2870, 3575, 3799
Eco32I GATATC 1 cut(s) 3317
Eco47I GGWCC 3 cut(s) 1626, 2253, 3861
Eco57I CTGAAG 8 cut(s) 410, 570, 1674, 2373, 3287, 3470, 3670, 3740
Eco72I CACGTG 2 cut(s) 3179, 3483
Eco88I CYCGRG 1 cut(s) 914
EcoO109I RGGNCCY 1 cut(s) 3861
EcoRI GAATTC 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 1622
EcoRV GATATC 1 cut(s) 3317
EcoT14I CCWWGG 2 cut(s) 1661, 2364
EcoT22I ATGCAT 1 cut(s) 1417
EcoT38I GRGCYC 3 cut(s) 2870, 3575, 3799
ErhI CCWWGG 2 cut(s) 1661, 2364
Esp3I CGTCTC 1 cut(s) 401
FalI AAGNNNNNCTT 4 cut(s) 1156, 1188, 3572, 3604
FaqI GGGAC 3 cut(s) 96, 600, 3874
FauNDI CATATG 1 cut(s) 3421
FbaI TGATCA 5 cut(s) 1567, 1717, 2527, 3001, 3202
FblI GTMKAC 1 cut(s) 1421
Fnu4HI GCNGC 5 cut(s) 1286, 2902, 3308, 3440, 4179
FriOI GRGCYC 3 cut(s) 2870, 3575, 3799
Fsp4HI GCNGC 5 cut(s) 1286, 2902, 3308, 3440, 4179
GlaI GCGC 1 cut(s) 523
GluI GCNGC 5 cut(s) 1286, 2902, 3308, 3440, 4179
GsaI CCCAGC 3 cut(s) 2310, 3284, 3736
GsuI CTGGAG 1 cut(s) 324
HaeIII GGCC 6 cut(s) 336, 2363, 3293, 3487, 3573, 3942
HapII CCGG 8 cut(s) 822, 1522, 3464, 3930, 3990, 4027, 4061, 4254
HgaI GACGC 3 cut(s) 93, 145, 383
HhaI GCGC 1 cut(s) 524
Hin1I GRCGYC 2 cut(s) 73, 85
Hin6I GCGC 1 cut(s) 522
HinP1I GCGC 1 cut(s) 522
HincII GTYRAC 4 cut(s) 450, 2154, 3034, 3184
HindII GTYRAC 4 cut(s) 450, 2154, 3034, 3184
HindIII AAGCTT 3 cut(s) 1242, 2675, 4099
HpaI GTTAAC 2 cut(s) 2154, 3034
HpaII CCGG 8 cut(s) 822, 1522, 3464, 3930, 3990, 4027, 4061, 4254
HphI GGTGA 6 cut(s) 218, 1712, 1718, 2010, 3371, 4181
Hpy166II GTNNAC 7 cut(s) 54, 450, 1422, 2154, 3034, 3184, 3637
Hpy8I GTNNAC 7 cut(s) 54, 450, 1422, 2154, 3034, 3184, 3637
Hpy99I CGWCG 1 cut(s) 161
HpyF10VI GCNNNNNNNGC 5 cut(s) 827, 1291, 1412, 3290, 4237
Hsp92I GRCGYC 2 cut(s) 73, 85
HspAI GCGC 1 cut(s) 522
KflI GGGWCCC 1 cut(s) 3861
Kpn2I TCCGGA 4 cut(s) 1521, 3929, 4026, 4253
Ksp22I TGATCA 5 cut(s) 1567, 1717, 2527, 3001, 3202
KspAI GTTAAC 2 cut(s) 2154, 3034
LguI GCTCTTC 2 cut(s) 342, 820
LmnI GCTCC 2 cut(s) 3802, 4256
Lsp1109I GCAGC 5 cut(s) 1297, 2888, 3319, 3426, 4190
LweI GCATC 6 cut(s) 551, 709, 1850, 2170, 3919, 4165
MaeIII GTNAC 9 cut(s) 23, 694, 1066, 1141, 1265, 1800, 2801, 2920, 3744
MfeI CAATTG 3 cut(s) 18, 1274, 3594
MflI RGATCY 3 cut(s) 576, 1075, 1617
MhlI GDGCHC 4 cut(s) 2870, 3575, 3724, 3799
MlsI TGGCCA 2 cut(s) 3293, 3487
MluNI TGGCCA 2 cut(s) 3293, 3487
MmeI TCCRAC 8 cut(s) 16, 1554, 1755, 1848, 1867, 1962, 1971, 3133
Mox20I TGGCCA 2 cut(s) 3293, 3487
Mph1103I ATGCAT 1 cut(s) 1417
MroI TCCGGA 4 cut(s) 1521, 3929, 4026, 4253
MroXI GAANNNNTTC 7 cut(s) 681, 762, 1686, 2418, 2700, 2792, 4322
MscI TGGCCA 2 cut(s) 3293, 3487
MslI CAYNNNNRTG 4 cut(s) 222, 671, 1701, 2886
Msp20I TGGCCA 2 cut(s) 3293, 3487
MspCI CTTAAG 2 cut(s) 1289, 1439
MspI CCGG 8 cut(s) 822, 1522, 3464, 3930, 3990, 4027, 4061, 4254
MspR9I CCNGG 4 cut(s) 1624, 3465, 3990, 4062
MunI CAATTG 3 cut(s) 18, 1274, 3594
Mva1269I GAATGC 4 cut(s) 1497, 2122, 2792, 3100
MvaI CCWGG 1 cut(s) 1624
MvnI CGCG 2 cut(s) 522, 3979
MwoI GCNNNNNNNGC 5 cut(s) 827, 1291, 1412, 3290, 4237
NciI CCSGG 3 cut(s) 3465, 3990, 4062
NdeI CATATG 1 cut(s) 3421
NlaIV GGNNCC 4 cut(s) 3573, 3798, 3862, 3863
NmeAIII GCCGAG 2 cut(s) 73, 4434
NmuCI GTSAC 3 cut(s) 1141, 2801, 3744
NruI TCGCGA 1 cut(s) 3979
NsiI ATGCAT 1 cut(s) 1417
NspI RCATGY 3 cut(s) 1485, 2668, 3854
OliI CACNNNNGTG 1 cut(s) 671
PagI TCATGA 1 cut(s) 258
PciI ACATGT 2 cut(s) 1481, 2664
PciSI GCTCTTC 2 cut(s) 342, 820
PctI GAATGC 4 cut(s) 1497, 2122, 2792, 3100
PdmI GAANNNNTTC 7 cut(s) 681, 762, 1686, 2418, 2700, 2792, 4322
PflMI CCANNNNNTGG 1 cut(s) 1396
PfoI TCCNGGA 2 cut(s) 3463, 3988
PkrI GCNGC 5 cut(s) 1287, 2903, 3309, 3441, 4180
PmaCI CACGTG 2 cut(s) 3179, 3483
PmlI CACGTG 2 cut(s) 3179, 3483
Ppu21I YACGTR 2 cut(s) 3179, 3483
PpuMI RGGWCCY 1 cut(s) 3861
PscI ACATGT 2 cut(s) 1481, 2664
PshAI GACNNNNGTC 1 cut(s) 3179
PshBI ATTAAT 3 cut(s) 2385, 3162, 3528
PsiI TTATAA 1 cut(s) 2768
Psp1406I AACGTT 1 cut(s) 3036
Psp5II RGGWCCY 1 cut(s) 3861
Psp6I CCWGG 1 cut(s) 1622
PspCI CACGTG 2 cut(s) 3179, 3483
PspFI CCCAGC 3 cut(s) 2306, 3280, 3732
PspGI CCWGG 1 cut(s) 1622
PspN4I GGNNCC 4 cut(s) 3573, 3798, 3862, 3863
PspOMI GGGCCC 1 cut(s) 3571
PspPI GGNCC 6 cut(s) 1626, 2253, 3571, 3572, 3861, 3941
PspPPI RGGWCCY 1 cut(s) 3861
PstNI CAGNNNCTG 1 cut(s) 2183
PsuI RGATCY 3 cut(s) 576, 1075, 1617
RruI TCGCGA 1 cut(s) 3979
RseI CAYNNNNRTG 4 cut(s) 222, 671, 1701, 2886
SapI GCTCTTC 2 cut(s) 342, 820
SatI GCNGC 5 cut(s) 1286, 2902, 3308, 3440, 4179
Sau96I GGNCC 6 cut(s) 1626, 2253, 3571, 3572, 3861, 3941
ScrFI CCNGG 4 cut(s) 1624, 3465, 3990, 4062
SduI GDGCHC 4 cut(s) 2870, 3575, 3724, 3799
SfaNI GCATC 6 cut(s) 551, 709, 1850, 2170, 3919, 4165
SfcI CTRYAG 1 cut(s) 2605
SinI GGWCC 3 cut(s) 1626, 2253, 3861
SmiMI CAYNNNNRTG 4 cut(s) 222, 671, 1701, 2886
SmlI CTYRAG 9 cut(s) 138, 1101, 1289, 1439, 1766, 2056, 2509, 2588, 3241
SmoI CTYRAG 9 cut(s) 138, 1101, 1289, 1439, 1766, 2056, 2509, 2588, 3241
SpeI ACTAGT 1 cut(s) 670
SsiI CCGC 3 cut(s) 1975, 3071, 4306
SspI AATATT 3 cut(s) 3772, 3813, 4426
StyD4I CCNGG 4 cut(s) 1622, 3463, 3988, 4060
StyI CCWWGG 2 cut(s) 1661, 2364
TaqI TCGA 6 cut(s) 240, 964, 1408, 2049, 3117, 4350
TatI WGTACW 5 cut(s) 51, 1504, 1608, 2822, 3219
TscAI CASTG 6 cut(s) 520, 625, 1401, 3212, 3826, 4095
TseFI GTSAC 3 cut(s) 1141, 2801, 3744
TseI GCWGC 5 cut(s) 1285, 2901, 3307, 3439, 4178
Tsp45I GTSAC 3 cut(s) 1141, 2801, 3744
TspGWI ACGGA 4 cut(s) 1479, 2736, 3622, 4379
TspRI CASTG 6 cut(s) 520, 625, 1401, 3212, 3826, 4095
Van91I CCANNNNNTGG 1 cut(s) 1396
Vha464I CTTAAG 2 cut(s) 1289, 1439
VpaK11BI GGWCC 3 cut(s) 1626, 2253, 3861
VspI ATTAAT 3 cut(s) 2385, 3162, 3528
XbaI TCTAGA 3 cut(s) 956, 2686, 3967
XceI RCATGY 3 cut(s) 1485, 2668, 3854
XcmI CCANNNNNNNNNTGG 1 cut(s) 2237
XmiI GTMKAC 1 cut(s) 1421
XmnI GAANNNNTTC 7 cut(s) 681, 762, 1686, 2418, 2700, 2792, 4322
ZraI GACGTC 1 cut(s) 74
Zsp2I ATGCAT 1 cut(s) 1417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.