Rroxscaffold_4G00317230
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000639
Physical Location & Seq
Forward (+)
42361079 .. 42374510
13432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00317230.1

Sequence Viewer

Length: 3594 bp
ATGAGCTGCGACCATTTCCCAGTGCCACTGGAATACCGATTCCGTCCCACCAAAAAGGAGCTGCTGTGTGACCACTTGGCGAAGAAGAATCGGTGCAAGGACTCCCAAATCATGTCCATCATCCCAGAAATCGATCTGTGCAGGCACGAGCCTCGTGAGCTACCAGGTAATCCATCACCGGAACCAGAGCACCACCAATTTCAATTATTATCAATGATCCAGTCATGTGTATTGTGTTGCTTCTTTGCAGGACTGGCGTACACAAGGCAGGAAAATCTGGGAAGGAAACTTTACTTCGATTTCTTCTTTCGGTTTACAAGGGCAGAGTCTTGGTATAGTCAGGGCAAGTGGTACTTCTTCGCACCTCGTGAATACAAGTACCCTAACAGCACTCGCTCCAACAGGACTACAGGGGAAGGGTCCTGGAAGGTCACAGGCAAGGACCGCGACATCAAGGCCCCGGGCTCCGAAGCTGTGATTGGGAGAAAGAGGATCTTGACCTTCCAAAAACGTGGAGTGCCGAAATCTCACAAGACCGGCTATGTTATTCATGAGTATTATCACATTCAGCCCCATTCTGACCCTCCTAAGCAGATAGGGGACTTTGTTGTCTGTTGCTTGAAGTACAAGCCAGACAACTCTGATCATGATGAGGATGCTCCATTCTGCAATCGAGGTAGTGGTAGCGGTAGCGGTAGCTGCAGTATGGTTTCCAATGTTGCAAATCAGGCTGAAGAAAATTTGGCGTCCAATGAGCAGTTTAATGGCACTGATGATGAAGCTGAAACTGGTGGCGGTTGGATTACTGAAGCAGAACAACACCTGGCATATAAGAAGCTGAGGGATGATCGTCGGACTCCCATTGGAAATCAGAATGAATTCCAAGGCTCGCAGACTGATCCTTGTACTCTTTGGACCCCTGTTGAAGGTCAAGCTGATAGAAGCAATTCGTCTCATATGATTGAAGAGCGACTAAATGATCCAGAAAATATGGATTCATTCTTTCATCCACCTGTGCCCCCTCAACTGCATCAGCTACATCAGTCACCGATGTATACCACCAATGTCCAGAATGATGAATACTATAAGAGGAAATATCCATTTGAAGATAATGACTCTTCTCTTGCGAAGAAGAACAATATTTCAACCAATGATGGTGATGAACTAGTCAGCAACACCACATCTAATTCTGAAAACCAAGCTGCAGATGTGATCCTAGAGGGTGACTCTGTTATCTGTCGCTTGAAGAATAAATCAAATAAGAAGGATTCTCTGGTAAGTGATGAAGGGGAACCGGATAGCGCAGGTGTGTGTAACTGGAAAATTCAAGCGGCACACAAAAATGATGAACCACTTTTACATCAACCTCAGCGTCTGGATGACTGCTGCTCCTCTGCAATGCGGTCTCCGGCATCCCCAGAGCTGGAAGCTGTTCTGCAATTAGCCAATGGTGATGATTGTAATAAGTTGCAATCACCATGTGGAGATAATACTTCTTGTCTAGAAGACAAGAATGAAGTTTCAACCTGTGATGAAGATGAGACTATGGATGGTGTGTTTCCACAGCTATGTGACTTACCAGAAGTAAATCTTGATTTATTTTTGCGTCCAATTCAGCCACAAGATTACCGCCCCTCCATACTGCAGTCATCAATATACACAAAGCTGGGAAATGTTCCAGATGCCAATCTCTGTTTTAGTGAATGCAAGAATTGGCAATCTGCATTTAAGAAAAAAAATTCAACTAATGAAGTTGACATACCGGTTAGACATATCATGTCTAATTTTGAAAATCAATCTACAGATTCTAGGATTCTAGAGGTGCATCATCAAACCAAAGAAAATATGGAATCAACGTTTCATCCATTGCAGCTAGAAGATTCAGCTCTGCAATCGATGATGTACACAAAACATGGAGATGTTCTACATAATATTGAATGCAATGAGTTGCAATCTTACTGTTCTGATCAAATAGCTCAAACTCCTTCCATAGACTCCAACCTACCGAACTCATTGGGAAGGGTCTACCATGGGGATAGTGGAGTAAGCAGCTATGATACGAACACTGAAGTCGCCCATGGATGGGAACAACATACATCTGCATTAGCTATTAGAAACCCTCCAATAGTGAAAGAGATGGGCTGCGGCGGCTTCACAGTTCCTCCGGGATACGGATTCCATCCCACCGACGAGAAGCTGCTTAGTGACTTGGAGGAGAAGAATCAGTGCAAGGACTCCGAGATCACCACCATCATCCCTGAAATCGATGTGTGCAAACACGAGCCTCACGAGTTACCAGAACTGGCATTCACAAGGGCGCAAAATCTGGGCAGGAAGTTTTTCTCTTTTTTCTTCTTCTGGTTTGCATGGGCAGAGTCTTGGGACAGTCAGGACAAGTGGTACTTCTTCACTCCGCGTGAATACAAGTACCCTAACAGCAGACGCTCCAACAGGACTACAGGGGAAGGGTTCTGGAAGATCACAGGCAAGGACAGAGACATCAAGGCCCTTCGCTCCAAAGCTGTGATTGGGAGAAAGAGGACCTTGACCTTCTACAAACGTGGAGTGCCGAAATCCCAGAAGACTGGCTGGGTCATTCATGAGTATTATCTCATTCAGGCACATTCTGATCCTCCTAAGCAGATAGGGGACTTTGTAGTCTGTTGCTTGAAGTATAAGTCAGACAACTCTGATCATGATAAGGATGCCCCATTCTGCGATCGAGGTAGCAGCAGCTGCAGTATGGCCTCCAATGTTGAAAATCAAGCTGAAGAAAATTGGGCATCCATTAAGCAGGTTAACGTTGTTCCAATTCCCAATGGAAATGATGATGAAGCTGAAATTGGTGGTGGTACGATTACAGAAGAAGAGGAATACCTGGCTTTAAAGGAGCTGGAAGATGCTCTTCAAATTCCCATTGGCAATCCTCATGACTTCCAAGGCATGCAAACTGAGCCTTGTCATTTAGATGAGAATGATCTTTCAACCTCTGTTGAAGGCCGACCTGATAAAAGCAATTCATCTGATATGATTGAAGAGCTACTAGATGACCCACAAAATCTGGATTTACTCTTTCATCCGCCTGCGCCACCTCAACTGCATCAGCTACATCAGTCACCCATGTATACCACCAATGTCCATAATGATGAATGTCGTAAGAGGACATATCCATTTGAGGGTAGTGACCCTTCTCTTACAAAGAAGAACAATATTTCAACCAATGATGATGACGAATTAGTTAGCAATACCCCATCTAGTTCTGAAAATAAAGCTGCGAATATGATTCCAGAGGGATATCCTCAACCAGAAGCAAATCTAGGATCAGAAGTTGAATCATTTCTGCCAAAGGATTACGATTATACACTACCATCAATAAACGGAGGACCGGGAGATTTTCCCCATGACAATAATTTTATTGAGTGGGATGATTTGCCATCCTTATACGAAGGTATGGACTCTTCTCCCGTAAAGTTCTCACATACAGTTATTGATGGTGGTGGAAGAGTAACCAATGACGGAAACACCGAAGTAGTAGATGGATCAATAAACGATTATTCTGCATTAGCCATTGGAGCAACGCTCCAGAGTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

1197

Amino Acids

135.07

Weight (kDa)

4.93

Isoelectric Point (pI)

53.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 10 - 58 2.1e-07 No apical meristem (NAM) protein
NAM PF02365 113 - 186 2.6e-16 No apical meristem (NAM) protein
NAM PF02365 721 - 870 1.3e-28 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000639)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020
rosa_chinensis RchiOBHm_Chr1g0324821 RchiOBHm_Chr1g0333801 RchiOBHm_Chr1g0361601 RchiOBHm_Chr1g0361611 RchiOBHm_Chr1g0361661
rosa_laevigata RLG00000027711 RLG00000027712 RLG00000027714 RLG00000027715 RLG00000027716 RLG00000029530 RLG00000029531 RLG00000030172
rosa_multiflora Rmu_co8302323.1_g000001 Rmu_co8459497.1_g000001 Rmu_sc0003426.1_g000013 Rmu_sc0003693.1_g000002 Rmu_sc0003693.1_g000003 Rmu_sc0003704.1_g000001 Rmu_sc0003704.1_g000002 Rmu_sc0006101.1_g000001 Rmu_sc0006101.1_g000010 Rmu_sc0006417.1_g000001 Rmu_sc0006417.1_g000002 Rmu_sc0006417.1_g000005 Rmu_sc0007217.1_g000011 Rmu_sc0010981.1_g000006 Rmu_sc0011258.1_g000006 Rmu_sc0017253.1_g000003 Rmu_sc0017253.1_g000004 Rmu_sc0017750.1_g000001 Rmu_sc0026316.1_g000001 Rmu_sc0038193.1_g000002 Rmu_sc0041549.1_g000001
rosa_roxburghii Rroxscaffold_159G00433000 Rroxscaffold_159G00433050 Rroxscaffold_159G00433080 Rroxscaffold_4G00294830 Rroxscaffold_4G00294840 Rroxscaffold_4G00294900 Rroxscaffold_4G00294910 Rroxscaffold_4G00294920 Rroxscaffold_4G00317230 Rroxscaffold_4G00325170 Rroxscaffold_4G00325200 Rroxscaffold_4G00325280
rosa_rugosa Rorug01G0048000 Rorug01G0111600 Rorug01G0111700 Rorug01G0290200 Rorug01G0290300 Rorug01G0290300 Rorug01G0290400 Rorug01G0290600
rosa_samantha Rh1AG064300 Rh1AG064500 Rh1AG134000 Rh1AG134100 Rh1AG300500 Rh1AG300600
rosa_wichuraiana Rw1G005340 Rw1G005390 Rw1G005420 Rw1G011180 Rw1G011190 Rw1G026650 Rw1G026660 Rw1G026680 Rw1G026690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1295
AasI GACNNNNNNGTC 2 cut(s) 608, 2657
Acc36I ACCTGC 2 cut(s) 1295, 2785
AccI GTMKAC 3 cut(s) 1055, 2025, 3125
AccII CGCG 2 cut(s) 447, 2416
AclI AACGTT 2 cut(s) 1856, 2802
AclWI GGATC 8 cut(s) 211, 500, 893, 974, 1207, 2624, 3328, 3547
AcsI RAATTY 5 cut(s) 739, 878, 1323, 1738, 2910
AcuI CTGAAG 4 cut(s) 753, 828, 2088, 2790
AcyI GRCGYC 1 cut(s) 746
AdeI CACNNNGTG 2 cut(s) 368, 1481
AfaI GTAC 9 cut(s) 260, 353, 380, 626, 907, 1904, 2402, 2429, 2854
AfiI CCNNNNNNNGG 6 cut(s) 926, 1423, 2082, 2083, 2171, 3176
AgeI ACCGGT 1 cut(s) 1762
AhlI ACTAGT 1 cut(s) 1165
AjnI CCWGG 4 cut(s) 163, 422, 822, 2877
AjuI GAANNNNNNNTTGG 4 cut(s) 462, 494, 2826, 2858
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 4 cut(s) 957, 1410, 1535, 2490
AlwI GGATC 8 cut(s) 211, 500, 893, 974, 1207, 2624, 3328, 3547
AlwNI CAGNNNCTG 2 cut(s) 1375, 2736
Ama87I CYCGRG 1 cut(s) 460
AoxI GGCC 4 cut(s) 456, 2505, 2745, 2998
ApoI RAATTY 5 cut(s) 739, 878, 1323, 1738, 2910
AsiGI ACCGGT 1 cut(s) 1762
Asp700I GAANNNNTTC 5 cut(s) 878, 946, 1431, 2339, 3336
AspLEI GCGC 3 cut(s) 1304, 2320, 3088
AspS9I GGNCC 7 cut(s) 420, 442, 457, 915, 2506, 2541, 3383
AsuC2I CCSGG 4 cut(s) 461, 462, 2166, 3387
AsuHPI GGTGA 8 cut(s) 168, 1038, 1169, 1235, 1463, 1467, 2236, 3108
AvaI CYCGRG 1 cut(s) 460
AvaII GGWCC 5 cut(s) 420, 442, 915, 2541, 3383
BaeGI GKGCMC 1 cut(s) 1020
BanII GRGCYC 1 cut(s) 467
BarI GAAGNNNNNNTAC 6 cut(s) 1743, 1775, 3172, 3204, 3442, 3474
BauI CACGAG 5 cut(s) 146, 153, 366, 2279, 2288
BbsI GAAGAC 2 cut(s) 1512, 2588
Bbv12I GWGCWC 1 cut(s) 192
BbvCI CCTCAGC 2 cut(s) 839, 1368
BcgI CGANNNNNNTGC 4 cut(s) 134, 168, 3539, 3573
BciT130I CCWGG 4 cut(s) 165, 424, 824, 2879
BciVI GTATCC 1 cut(s) 2162
BclI TGATCA 3 cut(s) 643, 1966, 2692
BcnI CCSGG 4 cut(s) 461, 462, 2166, 3387
BcoDI GTCTC 4 cut(s) 957, 1410, 1535, 2490
BcuI ACTAGT 1 cut(s) 1165
BfaI CTAG 9 cut(s) 1166, 1217, 1502, 1809, 1817, 1874, 3044, 3255, 3317
BfmI CTRYAG 7 cut(s) 408, 700, 1203, 1643, 1800, 2457, 2737
BfuAI ACCTGC 2 cut(s) 1295, 2785
BfuI GTATCC 1 cut(s) 2162
Bme1390I CCNGG 8 cut(s) 165, 424, 461, 462, 824, 2166, 2879, 3387
Bme18I GGWCC 5 cut(s) 420, 442, 915, 2541, 3383
BmeT110I CYCGRG 1 cut(s) 460
BmgT120I GGNCC 7 cut(s) 420, 442, 457, 915, 2506, 2541, 3383
BmiI GGNNCC 6 cut(s) 183, 421, 459, 466, 917, 1293
BmrFI CCNGG 8 cut(s) 165, 424, 461, 462, 824, 2166, 2879, 3387
BmrI ACTGGG 1 cut(s) 14
BmsI GCATC 9 cut(s) 646, 1039, 1421, 1672, 1834, 2695, 2792, 2890, 3109
BmuI ACTGGG 1 cut(s) 14
BpiI GAAGAC 2 cut(s) 1512, 2588
BplI GAGNNNNNCTC 3 cut(s) 1211, 1243, 3564
BpmI CTGGAG 1 cut(s) 3566
Bpu10I CCTNAGC 4 cut(s) 588, 839, 1368, 2637
BpuMI CCSGG 4 cut(s) 461, 462, 2166, 3387
Bsa29I ATCGAT 3 cut(s) 132, 1895, 2265
BsaBI GATNNNNATC 1 cut(s) 1686
BsaHI GRCGYC 1 cut(s) 746
BsaI GGTCTC 1 cut(s) 1410
BsaJI CCNNGG 6 cut(s) 459, 460, 883, 2029, 2077, 2938
BsaWI WCCGGW 3 cut(s) 178, 1294, 1762
BsaXI ACNNNNNCTCC 8 cut(s) 50, 80, 1373, 1403, 1619, 1649, 2146, 2176
Bsc4I CCNNNNNNNGG 6 cut(s) 926, 1423, 2082, 2083, 2171, 3176
Bse118I RCCGGY 2 cut(s) 536, 1762
Bse1I ACTGG 8 cut(s) 20, 33, 220, 258, 793, 1322, 2307, 2590
Bse3DI GCAATG 3 cut(s) 1404, 1865, 1948
Bse8I GATNNNNATC 1 cut(s) 1686
BseBI CCWGG 4 cut(s) 165, 424, 824, 2879
BseCI ATCGAT 3 cut(s) 132, 1895, 2265
BseDI CCNNGG 6 cut(s) 459, 460, 883, 2029, 2077, 2938
BseJI GATNNNNATC 1 cut(s) 1686
BseLI CCNNNNNNNGG 6 cut(s) 926, 1423, 2082, 2083, 2171, 3176
BseMI GCAATG 3 cut(s) 1404, 1865, 1948
BseMII CTCAG 3 cut(s) 830, 1382, 2943
BseNI ACTGG 8 cut(s) 20, 33, 220, 258, 793, 1322, 2307, 2590
BseRI GAGGAG 2 cut(s) 1381, 2228
BseSI GKGCMC 1 cut(s) 1020
BseYI CCCAGC 2 cut(s) 1666, 2589
BsgI GTGCAG 1 cut(s) 160
Bsh1236I CGCG 2 cut(s) 447, 2416
Bsh1285I CGRYCG 1 cut(s) 2722
BshFI GGCC 4 cut(s) 458, 2507, 2747, 3000
BshTI ACCGGT 1 cut(s) 1762
BshVI ATCGAT 3 cut(s) 132, 1895, 2265
BsiEI CGRYCG 1 cut(s) 2722
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 460
BsiSI CCGG 8 cut(s) 179, 461, 537, 1295, 1409, 1763, 2165, 3386
BslFI GGGAC 4 cut(s) 30, 614, 2396, 2663
BslI CCNNNNNNNGG 6 cut(s) 926, 1423, 2082, 2083, 2171, 3176
BsmAI GTCTC 4 cut(s) 957, 1410, 1535, 2490
BsmBI CGTCTC 1 cut(s) 957
BsmFI GGGAC 4 cut(s) 30, 614, 2396, 2663
BsmI GAATGC 3 cut(s) 1709, 1943, 2306
BsnI GGCC 4 cut(s) 458, 2507, 2747, 3000
Bso31I GGTCTC 1 cut(s) 1410
BsoBI CYCGRG 1 cut(s) 460
Bsp1286I GDGCHC 3 cut(s) 192, 467, 1020
Bsp1407I TGTACA 1 cut(s) 1902
Bsp19I CCATGG 2 cut(s) 2029, 2077
BspANI GGCC 4 cut(s) 458, 2507, 2747, 3000
BspCNI CTCAG 3 cut(s) 831, 1381, 2944
BspDI ATCGAT 3 cut(s) 132, 1895, 2265
BspFNI CGCG 2 cut(s) 447, 2416
BspHI TCATGA 5 cut(s) 550, 646, 2599, 2695, 2929
BspLI GGNNCC 6 cut(s) 183, 421, 459, 466, 917, 1293
BspMAI CTGCAG 4 cut(s) 704, 1207, 1647, 2741
BspMI ACCTGC 2 cut(s) 1295, 2785
BspPI GGATC 8 cut(s) 211, 500, 893, 974, 1207, 2624, 3328, 3547
BspQI GCTCTTC 3 cut(s) 960, 2910, 3030
BspTNI GGTCTC 1 cut(s) 1410
BsrDI GCAATG 3 cut(s) 1404, 1865, 1948
BsrFI RCCGGY 2 cut(s) 536, 1762
BsrGI TGTACA 1 cut(s) 1902
BsrI ACTGG 8 cut(s) 20, 33, 220, 258, 793, 1322, 2307, 2590
BssAI RCCGGY 2 cut(s) 536, 1762
BssECI CCNNGG 6 cut(s) 459, 460, 883, 2029, 2077, 2938
BssNAI GTATAC 2 cut(s) 1056, 3126
BssNI GRCGYC 1 cut(s) 746
BssSI CACGAG 5 cut(s) 146, 153, 366, 2279, 2288
BssT1I CCWWGG 4 cut(s) 883, 2029, 2077, 2938
Bst1107I GTATAC 2 cut(s) 1056, 3126
Bst2BI CACGAG 5 cut(s) 146, 153, 366, 2279, 2288
Bst2UI CCWGG 4 cut(s) 165, 424, 824, 2879
Bst4CI ACNGT 4 cut(s) 1961, 2158, 2387, 3484
Bst6I CTCTTC 7 cut(s) 960, 1123, 2862, 2910, 3030, 3463, 3496
BstACI GRCGYC 1 cut(s) 746
BstAPI GCANNNNNTGC 1 cut(s) 2736
BstAUI TGTACA 1 cut(s) 1902
BstC8I GCNNGC 4 cut(s) 143, 890, 2945, 3084
BstDEI CTNAG 6 cut(s) 588, 839, 1368, 2201, 2637, 2952
BstDSI CCRYGG 2 cut(s) 2029, 2077
BstENI CCTNNNNNAGG 1 cut(s) 924
BstFNI CGCG 2 cut(s) 447, 2416
BstHHI GCGC 3 cut(s) 1304, 2320, 3088
BstMAI GTCTC 4 cut(s) 957, 1410, 1535, 2490
BstMCI CGRYCG 1 cut(s) 2722
BstMWI GCNNNNNNNGC 9 cut(s) 157, 254, 444, 699, 728, 2148, 2736, 2953, 3572
BstNI CCWGG 4 cut(s) 165, 424, 824, 2879
BstNSI RCATGY 1 cut(s) 2947
BstSCI CCNGG 8 cut(s) 163, 422, 459, 460, 822, 2164, 2877, 3385
BstSFI CTRYAG 7 cut(s) 408, 700, 1203, 1643, 1800, 2457, 2737
BstSLI GKGCMC 1 cut(s) 1020
BstUI CGCG 2 cut(s) 447, 2416
BstV2I GAAGAC 2 cut(s) 1512, 2588
BstX2I RGATCY 1 cut(s) 492
BstXI CCANNNNNNTGG 2 cut(s) 512, 2585
BstYI RGATCY 1 cut(s) 492
BstZ17I GTATAC 2 cut(s) 1056, 3126
Bsu15I ATCGAT 3 cut(s) 132, 1895, 2265
BsuI GTATCC 1 cut(s) 2162
BsuRI GGCC 4 cut(s) 458, 2507, 2747, 3000
BsuTUI ATCGAT 3 cut(s) 132, 1895, 2265
BtgI CCRYGG 2 cut(s) 2029, 2077
BtsIMutI CAGTG 5 cut(s) 26, 27, 768, 2064, 2231
BveI ACCTGC 2 cut(s) 1295, 2785
Cac8I GCNNGC 4 cut(s) 143, 890, 2945, 3084
CaiI CAGNNNCTG 2 cut(s) 1375, 2736
CciI TCATGA 5 cut(s) 550, 646, 2599, 2695, 2929
CfoI GCGC 3 cut(s) 1304, 2320, 3088
Cfr10I RCCGGY 2 cut(s) 536, 1762
Cfr13I GGNCC 7 cut(s) 420, 442, 457, 915, 2506, 2541, 3383
Cfr9I CCCGGG 1 cut(s) 460
ClaI ATCGAT 3 cut(s) 132, 1895, 2265
CseI GACGC 4 cut(s) 735, 1361, 1595, 2451
CsiI ACCWGGT 1 cut(s) 163
Csp6I GTAC 9 cut(s) 259, 352, 379, 625, 906, 1903, 2401, 2428, 2853
CspAI ACCGGT 1 cut(s) 1762
CviQI GTAC 9 cut(s) 259, 352, 379, 625, 906, 1903, 2401, 2428, 2853
DdeI CTNAG 6 cut(s) 588, 839, 1368, 2201, 2637, 2952
DraI TTTAAA 1 cut(s) 2886
DraIII CACNNNGTG 2 cut(s) 368, 1481
DrdI GACNNNNNNGTC 2 cut(s) 608, 2657
DseDI GACNNNNNNGTC 2 cut(s) 608, 2657
Eam1104I CTCTTC 7 cut(s) 960, 1123, 2862, 2910, 3030, 3463, 3496
EarI CTCTTC 7 cut(s) 960, 1123, 2862, 2910, 3030, 3463, 3496
EciI GGCGGA 1 cut(s) 3069
Eco130I CCWWGG 4 cut(s) 883, 2029, 2077, 2938
Eco24I GRGCYC 1 cut(s) 467
Eco31I GGTCTC 1 cut(s) 1410
Eco32I GATATC 1 cut(s) 3296
Eco47I GGWCC 5 cut(s) 420, 442, 915, 2541, 3383
Eco57I CTGAAG 4 cut(s) 753, 828, 2088, 2790
Eco88I CYCGRG 1 cut(s) 460
EcoNI CCTNNNNNAGG 1 cut(s) 924
EcoO109I RGGNCCY 4 cut(s) 420, 457, 2506, 2541
EcoRI GAATTC 1 cut(s) 878
EcoRII CCWGG 4 cut(s) 163, 422, 822, 2877
EcoRV GATATC 1 cut(s) 3296
EcoT14I CCWWGG 4 cut(s) 883, 2029, 2077, 2938
EcoT38I GRGCYC 1 cut(s) 467
ErhI CCWWGG 4 cut(s) 883, 2029, 2077, 2938
Esp3I CGTCTC 1 cut(s) 957
FaqI GGGAC 4 cut(s) 30, 614, 2396, 2663
FauNDI CATATG 1 cut(s) 957
FbaI TGATCA 3 cut(s) 643, 1966, 2692
FblI GTMKAC 3 cut(s) 1055, 2025, 3125
FriOI GRGCYC 1 cut(s) 467
FspBI CTAG 9 cut(s) 1166, 1217, 1502, 1809, 1817, 1874, 3044, 3255, 3317
GlaI GCGC 3 cut(s) 1303, 2319, 3087
GsaI CCCAGC 2 cut(s) 1670, 2593
GsuI CTGGAG 1 cut(s) 3566
HaeIII GGCC 4 cut(s) 458, 2507, 2747, 3000
HapII CCGG 8 cut(s) 179, 461, 537, 1295, 1409, 1763, 2165, 3386
HgaI GACGC 4 cut(s) 735, 1361, 1595, 2451
HhaI GCGC 3 cut(s) 1304, 2320, 3088
Hin1I GRCGYC 1 cut(s) 746
Hin6I GCGC 3 cut(s) 1302, 2318, 3086
HinP1I GCGC 3 cut(s) 1302, 2318, 3086
HincII GTYRAC 2 cut(s) 1756, 2800
HindII GTYRAC 2 cut(s) 1756, 2800
HpaI GTTAAC 1 cut(s) 2800
HpaII CCGG 8 cut(s) 179, 461, 537, 1295, 1409, 1763, 2165, 3386
HphI GGTGA 8 cut(s) 168, 1038, 1169, 1235, 1463, 1467, 2236, 3108
Hpy166II GTNNAC 8 cut(s) 261, 315, 1056, 1756, 1905, 2026, 2800, 3126
Hpy8I GTNNAC 8 cut(s) 261, 315, 1056, 1756, 1905, 2026, 2800, 3126
Hpy99I CGWCG 2 cut(s) 855, 2192
HpyCH4III ACNGT 4 cut(s) 1961, 2158, 2387, 3484
HpyCH4IV ACGT 4 cut(s) 511, 1856, 2560, 2802
HpyF10VI GCNNNNNNNGC 9 cut(s) 157, 254, 444, 699, 728, 2148, 2736, 2953, 3572
HpyF3I CTNAG 6 cut(s) 588, 839, 1368, 2201, 2637, 2952
HpySE526I ACGT 4 cut(s) 511, 1856, 2560, 2802
Hsp92I GRCGYC 1 cut(s) 746
HspAI GCGC 3 cut(s) 1302, 2318, 3086
Ksp22I TGATCA 3 cut(s) 643, 1966, 2692
KspAI GTTAAC 1 cut(s) 2800
LguI GCTCTTC 3 cut(s) 960, 2910, 3030
LweI GCATC 9 cut(s) 646, 1039, 1421, 1672, 1834, 2695, 2792, 2890, 3109
MabI ACCWGGT 1 cut(s) 163
MaeI CTAG 9 cut(s) 1166, 1217, 1502, 1809, 1817, 1874, 3044, 3255, 3317
MaeII ACGT 4 cut(s) 511, 1856, 2560, 2802
MflI RGATCY 1 cut(s) 492
MhlI GDGCHC 3 cut(s) 192, 467, 1020
MlyI GAGTC 9 cut(s) 95, 335, 850, 1109, 1220, 1988, 2228, 2384, 3449
MmeI TCCRAC 5 cut(s) 423, 779, 833, 2022, 2472
MroXI GAANNNNTTC 5 cut(s) 878, 946, 1431, 2339, 3336
MseI TTAA 5 cut(s) 762, 1728, 2790, 2799, 2885
MslI CAYNNNNRTG 5 cut(s) 1341, 1567, 1917, 2967, 3144
MspA1I CMGCKG 1 cut(s) 2736
MspI CCGG 8 cut(s) 179, 461, 537, 1295, 1409, 1763, 2165, 3386
MspR9I CCNGG 8 cut(s) 165, 424, 461, 462, 824, 2166, 2879, 3387
Mva1269I GAATGC 3 cut(s) 1709, 1943, 2306
MvaI CCWGG 4 cut(s) 165, 424, 824, 2879
MvnI CGCG 2 cut(s) 447, 2416
MwoI GCNNNNNNNGC 9 cut(s) 157, 254, 444, 699, 728, 2148, 2736, 2953, 3572
NciI CCSGG 4 cut(s) 461, 462, 2166, 3387
NcoI CCATGG 2 cut(s) 2029, 2077
NdeI CATATG 1 cut(s) 957
NlaIV GGNNCC 6 cut(s) 183, 421, 459, 466, 917, 1293
NmuCI GTSAC 8 cut(s) 68, 430, 1044, 1223, 1571, 2204, 3114, 3182
NspI RCATGY 1 cut(s) 2947
PaeI GCATGC 1 cut(s) 2947
PagI TCATGA 5 cut(s) 550, 646, 2599, 2695, 2929
PaqCI CACCTGC 1 cut(s) 1295
PciSI GCTCTTC 3 cut(s) 960, 2910, 3030
PcsI WCGNNNNNNNCGW 2 cut(s) 2286, 3522
PctI GAATGC 3 cut(s) 1709, 1943, 2306
PdmI GAANNNNTTC 5 cut(s) 878, 946, 1431, 2339, 3336
PfoI TCCNGGA 2 cut(s) 422, 2164
PinAI ACCGGT 1 cut(s) 1762
Ple19I CGATCG 1 cut(s) 2722
PleI GAGTC 9 cut(s) 95, 334, 850, 1109, 1220, 1988, 2228, 2383, 3449
PpsI GAGTC 9 cut(s) 95, 334, 850, 1109, 1220, 1988, 2228, 2383, 3449
PpuMI RGGWCCY 2 cut(s) 420, 2541
Psp1406I AACGTT 2 cut(s) 1856, 2802
Psp5II RGGWCCY 2 cut(s) 420, 2541
Psp6I CCWGG 4 cut(s) 163, 422, 822, 2877
PspFI CCCAGC 2 cut(s) 1666, 2589
PspGI CCWGG 4 cut(s) 163, 422, 822, 2877
PspN4I GGNNCC 6 cut(s) 183, 421, 459, 466, 917, 1293
PspPI GGNCC 7 cut(s) 420, 442, 457, 915, 2506, 2541, 3383
PspPPI RGGWCCY 2 cut(s) 420, 2541
PsrI GAACNNNNNNTAC 2 cut(s) 1341, 1373
PstI CTGCAG 4 cut(s) 704, 1207, 1647, 2741
PstNI CAGNNNCTG 2 cut(s) 1375, 2736
PsuI RGATCY 1 cut(s) 492
PvuI CGATCG 1 cut(s) 2722
PvuII CAGCTG 1 cut(s) 2736
RsaI GTAC 9 cut(s) 260, 353, 380, 626, 907, 1904, 2402, 2429, 2854
RsaNI GTAC 9 cut(s) 259, 352, 379, 625, 906, 1903, 2401, 2428, 2853
RseI CAYNNNNRTG 5 cut(s) 1341, 1567, 1917, 2967, 3144
SapI GCTCTTC 3 cut(s) 960, 2910, 3030
SaqAI TTAA 5 cut(s) 762, 1728, 2790, 2799, 2885
Sau96I GGNCC 7 cut(s) 420, 442, 457, 915, 2506, 2541, 3383
SchI GAGTC 9 cut(s) 95, 335, 850, 1109, 1220, 1988, 2228, 2384, 3449
ScrFI CCNGG 8 cut(s) 165, 424, 461, 462, 824, 2166, 2879, 3387
SduI GDGCHC 3 cut(s) 192, 467, 1020
SexAI ACCWGGT 1 cut(s) 163
SfaNI GCATC 9 cut(s) 646, 1039, 1421, 1672, 1834, 2695, 2792, 2890, 3109
SfcI CTRYAG 7 cut(s) 408, 700, 1203, 1643, 1800, 2457, 2737
SinI GGWCC 5 cut(s) 420, 442, 915, 2541, 3383
SmaI CCCGGG 1 cut(s) 462
SmiMI CAYNNNNRTG 5 cut(s) 1341, 1567, 1917, 2967, 3144
SpeI ACTAGT 1 cut(s) 1165
SphI GCATGC 1 cut(s) 2947
SspI AATATT 3 cut(s) 1141, 1933, 3211
SspMI CTAG 9 cut(s) 1166, 1217, 1502, 1809, 1817, 1874, 3044, 3255, 3317
StyD4I CCNGG 8 cut(s) 163, 422, 459, 460, 822, 2164, 2877, 3385
StyI CCWWGG 4 cut(s) 883, 2029, 2077, 2938
TaaI ACNGT 4 cut(s) 1961, 2158, 2387, 3484
TaiI ACGT 4 cut(s) 514, 1859, 2563, 2805
TaqI TCGA 6 cut(s) 132, 297, 673, 1895, 2265, 2722
TatI WGTACW 3 cut(s) 624, 905, 1902
TauI GCSGC 3 cut(s) 1334, 2148, 2151
Tru1I TTAA 5 cut(s) 762, 1728, 2790, 2799, 2885
Tru9I TTAA 5 cut(s) 762, 1728, 2790, 2799, 2885
TscAI CASTG 5 cut(s) 27, 33, 775, 2071, 2231
TseFI GTSAC 8 cut(s) 68, 430, 1044, 1223, 1571, 2204, 3114, 3182
Tsp45I GTSAC 8 cut(s) 68, 430, 1044, 1223, 1571, 2204, 3114, 3182
TspGWI ACGGA 4 cut(s) 32, 2187, 3393, 3531
TspMI CCCGGG 1 cut(s) 460
TspRI CASTG 5 cut(s) 27, 33, 775, 2071, 2231
VpaK11BI GGWCC 5 cut(s) 420, 442, 915, 2541, 3383
XagI CCTNNNNNAGG 1 cut(s) 924
XapI RAATTY 5 cut(s) 739, 878, 1323, 1738, 2910
XbaI TCTAGA 2 cut(s) 1501, 1816
XceI RCATGY 1 cut(s) 2947
XcmI CCANNNNNNNNNTGG 2 cut(s) 1843, 2036
XmaI CCCGGG 1 cut(s) 460
XmiI GTMKAC 3 cut(s) 1055, 2025, 3125
XmnI GAANNNNTTC 5 cut(s) 878, 946, 1431, 2339, 3336
XspI CTAG 9 cut(s) 1166, 1217, 1502, 1809, 1817, 1874, 3044, 3255, 3317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.