Rroxscaffold_159G00433080
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000159
OG0000639
Physical Location & Seq
Reverse (-)
794022 .. 797046
3025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_159G00433080.1

Sequence Viewer

Length: 1377 bp
ATGAGCTGCGGTGACTTCACAGTTCCGAAGGGATACCGATTCCATCCCTCCGACGAGGAGCTGCTTAGTCACTACTTGGAGGAGAAGAATCAGTCCAAGGACTCCCAGATCACGACCATCATCCCTGAAATCGATGTGTGCAAGCACGAACCTCGCGAGTTGCCAGAACTGGCGTTCACAAGGGCGGAAAGTCTGGGCAGGAAATTTTTCTTTTTCTTCTTTTTTTGGTTGGCAAGGGAAGTGTCTTGGGACAATCAAGGAAAGTGGTACTTCTTCACTCGGCGTGATTACAAGTACAATAACAGCACTCGCTCCAACAGGACTACAGGGGAAGGGTTTTGGAAGATCACAGGCAAGGACCGTGCAATCAAGGCCCCCCGCTCCAAAGCTGTCATTGGGAGAAAGAGGACATTGACCTTCTACAAACGTGGAGTGCCCAAATCCCAGAAGACCAGCTGGGTTCTTCATGAGTATTATCTCATTCAGGCACATTCTGAACCTCCTAAGCAGATAGGGGACTTTGTTGTCTGCTGCTTGAAGTACAAGTCAGACAACTCTGATTATGATAAGGATGCCCCATTCTGCGATCGAGGTAGCAGTAGCTGCAGTATGGCCTCCAATGTTGAAAATCAAGCTGAAGAAAATTGGGCATCGAAAGAGCTGGTTAATGTTGTTCCAATTCCCAGTGGAAATGATGATGAAGCTGAAACTGGTGGTGGTATGATTACAGAAGAAGAGGAATACCTGGCTTTAAAGGAGCTGAGAGACGCTCTTCAGATTCCCATTGGCAATCCACATGAATTCGAAGGCACGCAAACTGAACCTTGTCGTATAGATAAGAATGATCCTTCAACCAATAAGAATGATCCTTCAACTGCTGTTGAAGGCCAACCTGATAGAAGCAATTCATCTGATATGATTGACGAGCTACTAGATAACCCAGAAAATCTGGATTCACTCTTTCATCCGCCTGCACCACCTCAACTGCATCAGTCACCGATGCATACCACCAATATCCATAATGATGAATGTCGTAAGAGGACATATCCATTTGAGGGTAATGACCCTTCTCTTACGAAGAAGAATAATATCTCAACCAATGATAACAACGAATTAGTTAGCAACATCGCATCTAGTTCTGAAAATCAAGGCGCAAATATGATTCCAGAGGGATATTCTCAACTAGGAGCAAATCTGGGATCAGAATTTGATTCATTTCTGCCAAAGGAGTACGATTATACATTCAGTTACAGAAACACCGAAGTAGTACATGGATCGGCTGTGAAGCTGCAGGGGCAATATACTGCTGTTTTTCTGCCCCAGAAGAAACTGAAAGTCCTGGAAGCAGACAACAGATCAAAGCTGGCAGCAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

458

Amino Acids

52.07

Weight (kDa)

5.33

Isoelectric Point (pI)

50.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 9 - 159 4.1e-32 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000639)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020 FvH4_7g18020
rosa_chinensis RchiOBHm_Chr1g0324821 RchiOBHm_Chr1g0333801 RchiOBHm_Chr1g0361601 RchiOBHm_Chr1g0361611 RchiOBHm_Chr1g0361661
rosa_laevigata RLG00000027711 RLG00000027712 RLG00000027714 RLG00000027715 RLG00000027716 RLG00000029530 RLG00000029531 RLG00000030172
rosa_multiflora Rmu_co8302323.1_g000001 Rmu_co8459497.1_g000001 Rmu_sc0003426.1_g000013 Rmu_sc0003693.1_g000002 Rmu_sc0003693.1_g000003 Rmu_sc0003704.1_g000001 Rmu_sc0003704.1_g000002 Rmu_sc0006101.1_g000001 Rmu_sc0006101.1_g000010 Rmu_sc0006417.1_g000001 Rmu_sc0006417.1_g000002 Rmu_sc0006417.1_g000005 Rmu_sc0007217.1_g000011 Rmu_sc0010981.1_g000006 Rmu_sc0011258.1_g000006 Rmu_sc0017253.1_g000003 Rmu_sc0017253.1_g000004 Rmu_sc0017750.1_g000001 Rmu_sc0026316.1_g000001 Rmu_sc0038193.1_g000002 Rmu_sc0041549.1_g000001
rosa_roxburghii Rroxscaffold_159G00433000 Rroxscaffold_159G00433050 Rroxscaffold_159G00433080 Rroxscaffold_4G00294830 Rroxscaffold_4G00294840 Rroxscaffold_4G00294900 Rroxscaffold_4G00294910 Rroxscaffold_4G00294920 Rroxscaffold_4G00317230 Rroxscaffold_4G00325170 Rroxscaffold_4G00325200 Rroxscaffold_4G00325280
rosa_rugosa Rorug01G0048000 Rorug01G0111600 Rorug01G0111700 Rorug01G0290200 Rorug01G0290300 Rorug01G0290300 Rorug01G0290400 Rorug01G0290600
rosa_samantha Rh1AG064300 Rh1AG064500 Rh1AG134000 Rh1AG134100 Rh1AG300500 Rh1AG300600
rosa_wichuraiana Rw1G005340 Rw1G005390 Rw1G005420 Rw1G011180 Rw1G011190 Rw1G026650 Rw1G026660 Rw1G026680 Rw1G026690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 524
AccBSI CCGCTC 1 cut(s) 381
AccII CGCG 1 cut(s) 156
AciI CCGC 4 cut(s) 9, 185, 379, 968
AclWI GGATC 4 cut(s) 839, 860, 1207, 1282
AcsI RAATTY 3 cut(s) 203, 800, 1205
AcuI CTGAAG 2 cut(s) 657, 758
AfaI GTAC 5 cut(s) 269, 296, 542, 1232, 1269
AfiI CCNNNNNNNGG 1 cut(s) 1055
AgsI TTSAA 5 cut(s) 538, 626, 852, 873, 884
AjnI CCWGG 2 cut(s) 744, 1338
Alw26I GTCTC 1 cut(s) 759
AlwI GGATC 4 cut(s) 839, 860, 1207, 1282
AlwNI CAGNNNCTG 1 cut(s) 603
AoxI GGCC 3 cut(s) 372, 612, 886
ApeKI GCWGC 6 cut(s) 6, 61, 531, 603, 1288, 1367
ApoI RAATTY 3 cut(s) 203, 800, 1205
Asp700I GAANNNNTTC 2 cut(s) 206, 904
AspLEI GCGC 1 cut(s) 1154
AspS9I GGNCC 2 cut(s) 358, 373
AsuHPI GGTGA 2 cut(s) 23, 987
AsuII TTCGAA 1 cut(s) 804
AvaII GGWCC 1 cut(s) 358
BaeGI GKGCMC 1 cut(s) 438
BarI GAAGNNNNNNTAC 2 cut(s) 1051, 1083
BbsI GAAGAC 1 cut(s) 455
BbvI GCAGC 4 cut(s) 48, 518, 590, 1275
BccI CCATC 2 cut(s) 51, 125
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 2 cut(s) 746, 1340
BciVI GTATCC 1 cut(s) 26
BcoDI GTCTC 1 cut(s) 759
BfaI CTAG 3 cut(s) 932, 1134, 1184
BfmI CTRYAG 3 cut(s) 324, 604, 1289
BfuI GTATCC 1 cut(s) 26
BisI GCNGC 6 cut(s) 7, 62, 532, 604, 1289, 1368
BlsI GCNGC 6 cut(s) 8, 63, 533, 605, 1290, 1369
Bme1390I CCNGG 2 cut(s) 746, 1340
Bme18I GGWCC 1 cut(s) 358
BmgT120I GGNCC 2 cut(s) 358, 373
BmiI GGNNCC 1 cut(s) 375
BmrFI CCNGG 2 cut(s) 746, 1340
BmrI ACTGGG 1 cut(s) 678
BmsI GCATC 5 cut(s) 562, 659, 990, 997, 1139
BmuI ACTGGG 1 cut(s) 678
BpiI GAAGAC 1 cut(s) 455
BplI GAGNNNNNCTC 2 cut(s) 754, 786
Bpu10I CCTNAGC 1 cut(s) 504
Bpu14I TTCGAA 1 cut(s) 804
Bsa29I ATCGAT 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 96
Bsc4I CCNNNNNNNGG 1 cut(s) 1055
Bse1I ACTGG 3 cut(s) 174, 684, 715
BseBI CCWGG 2 cut(s) 746, 1340
BseCI ATCGAT 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 4 cut(s) 43, 120, 577, 964
BseLI CCNNNNNNNGG 1 cut(s) 1055
BseMII CTCAG 1 cut(s) 752
BseNI ACTGG 3 cut(s) 174, 684, 715
BseRI GAGGAG 2 cut(s) 71, 95
BseSI GKGCMC 1 cut(s) 438
BseXI GCAGC 4 cut(s) 48, 518, 590, 1275
BseYI CCCAGC 1 cut(s) 456
BsgI GTGCAG 1 cut(s) 957
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 589
BshFI GGCC 3 cut(s) 374, 614, 888
BshVI ATCGAT 1 cut(s) 132
BsiEI CGRYCG 1 cut(s) 589
BslFI GGGAC 2 cut(s) 263, 530
BslI CCNNNNNNNGG 1 cut(s) 1055
BsmAI GTCTC 1 cut(s) 759
BsmBI CGTCTC 1 cut(s) 759
BsmFI GGGAC 2 cut(s) 263, 530
BsnI GGCC 3 cut(s) 374, 614, 888
Bsp119I TTCGAA 1 cut(s) 804
Bsp1286I GDGCHC 1 cut(s) 438
Bsp143I GATC 8 cut(s) 108, 345, 586, 844, 865, 1199, 1274, 1355
Bsp68I TCGCGA 1 cut(s) 156
BspACI CCGC 4 cut(s) 9, 185, 379, 968
BspANI GGCC 3 cut(s) 374, 614, 888
BspCNI CTCAG 1 cut(s) 753
BspDI ATCGAT 1 cut(s) 132
BspFNI CGCG 1 cut(s) 156
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 1 cut(s) 375
BspMAI CTGCAG 2 cut(s) 608, 1293
BspPI GGATC 4 cut(s) 839, 860, 1207, 1282
BspQI GCTCTTC 1 cut(s) 777
BspT104I TTCGAA 1 cut(s) 804
BsrBI CCGCTC 1 cut(s) 381
BsrI ACTGG 3 cut(s) 174, 684, 715
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 8 cut(s) 108, 345, 586, 844, 865, 1199, 1274, 1355
BssT1I CCWWGG 1 cut(s) 96
Bst2UI CCWGG 2 cut(s) 746, 1340
Bst4CI ACNGT 2 cut(s) 22, 362
Bst6I CTCTTC 2 cut(s) 729, 777
BstAPI GCANNNNNTGC 1 cut(s) 603
BstBI TTCGAA 1 cut(s) 804
BstC8I GCNNGC 4 cut(s) 143, 812, 972, 1365
BstDEI CTNAG 3 cut(s) 65, 504, 761
BstF5I GGATG 4 cut(s) 43, 120, 577, 964
BstFNI CGCG 1 cut(s) 156
BstHHI GCGC 1 cut(s) 1154
BstKTI GATC 8 cut(s) 111, 348, 589, 847, 868, 1202, 1277, 1358
BstMAI GTCTC 1 cut(s) 759
BstMBI GATC 8 cut(s) 108, 345, 586, 844, 865, 1199, 1274, 1355
BstMCI CGRYCG 1 cut(s) 589
BstMWI GCNNNNNNNGC 3 cut(s) 371, 603, 1294
BstNI CCWGG 2 cut(s) 746, 1340
BstSCI CCNGG 2 cut(s) 744, 1338
BstSFI CTRYAG 3 cut(s) 324, 604, 1289
BstSLI GKGCMC 1 cut(s) 438
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 4 cut(s) 48, 518, 590, 1275
BstV2I GAAGAC 1 cut(s) 455
Bsu15I ATCGAT 1 cut(s) 132
BsuI GTATCC 1 cut(s) 26
BsuRI GGCC 3 cut(s) 374, 614, 888
BsuTUI ATCGAT 1 cut(s) 132
BtgZI GCGATG 1 cut(s) 1111
BtsCI GGATG 4 cut(s) 43, 120, 577, 964
BtsIMutI CAGTG 1 cut(s) 691
BtuMI TCGCGA 1 cut(s) 156
Cac8I GCNNGC 4 cut(s) 143, 812, 972, 1365
CaiI CAGNNNCTG 1 cut(s) 603
CciI TCATGA 1 cut(s) 466
CfoI GCGC 1 cut(s) 1154
Cfr13I GGNCC 2 cut(s) 358, 373
ClaI ATCGAT 1 cut(s) 132
CseI GACGC 1 cut(s) 776
Csp6I GTAC 5 cut(s) 268, 295, 541, 1231, 1268
CspCI CAANNNNNGTGG 2 cut(s) 245, 280
CviAII CATG 3 cut(s) 467, 797, 1271
CviQI GTAC 5 cut(s) 268, 295, 541, 1231, 1268
DdeI CTNAG 3 cut(s) 65, 504, 761
DpnI GATC 8 cut(s) 110, 347, 588, 846, 867, 1201, 1276, 1357
DpnII GATC 8 cut(s) 108, 345, 586, 844, 865, 1199, 1274, 1355
DraI TTTAAA 1 cut(s) 753
DrdI GACNNNNNNGTC 1 cut(s) 524
DseDI GACNNNNNNGTC 1 cut(s) 524
Eam1104I CTCTTC 2 cut(s) 729, 777
EarI CTCTTC 2 cut(s) 729, 777
EciI GGCGGA 2 cut(s) 200, 957
Eco130I CCWWGG 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 358
Eco57I CTGAAG 2 cut(s) 657, 758
EcoO109I RGGNCCY 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 800
EcoRII CCWGG 2 cut(s) 744, 1338
EcoT14I CCWWGG 1 cut(s) 96
EcoT22I ATGCAT 1 cut(s) 1005
ErhI CCWWGG 1 cut(s) 96
Esp3I CGTCTC 1 cut(s) 759
FaeI CATG 3 cut(s) 470, 800, 1274
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 2 cut(s) 263, 530
FatI CATG 3 cut(s) 466, 796, 1270
FauI CCCGC 1 cut(s) 386
Fnu4HI GCNGC 6 cut(s) 7, 62, 532, 604, 1289, 1368
FokI GGATG 4 cut(s) 30, 107, 584, 951
Fsp4HI GCNGC 6 cut(s) 7, 62, 532, 604, 1289, 1368
FspBI CTAG 3 cut(s) 932, 1134, 1184
GlaI GCGC 1 cut(s) 1153
GluI GCNGC 6 cut(s) 7, 62, 532, 604, 1289, 1368
GsaI CCCAGC 1 cut(s) 460
HaeIII GGCC 3 cut(s) 374, 614, 888
HgaI GACGC 1 cut(s) 776
HhaI GCGC 1 cut(s) 1154
Hin1II CATG 3 cut(s) 470, 800, 1274
Hin6I GCGC 1 cut(s) 1152
HinP1I GCGC 1 cut(s) 1152
HinfI GANTC 7 cut(s) 39, 88, 101, 778, 953, 1162, 1211
HphI GGTGA 2 cut(s) 23, 987
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 9 cut(s) 27, 52, 496, 550, 559, 777, 913, 1141, 1204
Hpy188III TCNNGA 5 cut(s) 112, 155, 467, 950, 1166
Hpy8I GTNNAC 1 cut(s) 177
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 8 cut(s) 22, 326, 427, 800, 858, 878, 879, 1077
HpyCH4III ACNGT 2 cut(s) 22, 362
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 7 cut(s) 141, 365, 606, 974, 988, 1003, 1291
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 603, 1294
HpyF3I CTNAG 3 cut(s) 65, 504, 761
HpySE526I ACGT 1 cut(s) 427
Hsp92II CATG 3 cut(s) 470, 800, 1274
HspAI GCGC 1 cut(s) 1152
Kzo9I GATC 8 cut(s) 108, 345, 586, 844, 865, 1199, 1274, 1355
LguI GCTCTTC 1 cut(s) 777
LmnI GCTCC 5 cut(s) 58, 317, 386, 757, 1187
Lsp1109I GCAGC 4 cut(s) 48, 518, 590, 1275
LweI GCATC 5 cut(s) 562, 659, 990, 997, 1139
MaeI CTAG 3 cut(s) 932, 1134, 1184
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 4 cut(s) 11, 68, 993, 1247
MalI GATC 8 cut(s) 110, 347, 588, 846, 867, 1201, 1276, 1357
MbiI CCGCTC 1 cut(s) 381
MboI GATC 8 cut(s) 108, 345, 586, 844, 865, 1199, 1274, 1355
MhlI GDGCHC 1 cut(s) 438
MluCI AATT 7 cut(s) 203, 643, 678, 800, 904, 1112, 1205
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 2 cut(s) 75, 339
Mph1103I ATGCAT 1 cut(s) 1005
MroXI GAANNNNTTC 2 cut(s) 206, 904
MseI TTAA 2 cut(s) 666, 752
MslI CAYNNNNRTG 1 cut(s) 1023
MspA1I CMGCKG 1 cut(s) 456
MspR9I CCNGG 2 cut(s) 746, 1340
MvaI CCWGG 2 cut(s) 746, 1340
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 3 cut(s) 371, 603, 1294
NdeII GATC 8 cut(s) 108, 345, 586, 844, 865, 1199, 1274, 1355
NlaIII CATG 3 cut(s) 470, 800, 1274
NlaIV GGNNCC 1 cut(s) 375
NmeAIII GCCGAG 1 cut(s) 259
NmuCI GTSAC 3 cut(s) 11, 68, 993
NruI TCGCGA 1 cut(s) 156
NsiI ATGCAT 1 cut(s) 1005
NspV TTCGAA 1 cut(s) 804
PagI TCATGA 1 cut(s) 466
PciSI GCTCTTC 1 cut(s) 777
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdmI GAANNNNTTC 2 cut(s) 206, 904
PfeI GAWTC 6 cut(s) 39, 88, 778, 953, 1162, 1211
PfoI TCCNGGA 1 cut(s) 1338
PkrI GCNGC 6 cut(s) 8, 63, 533, 605, 1290, 1369
Ple19I CGATCG 1 cut(s) 589
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
Psp6I CCWGG 2 cut(s) 744, 1338
PspFI CCCAGC 1 cut(s) 456
PspGI CCWGG 2 cut(s) 744, 1338
PspN4I GGNNCC 1 cut(s) 375
PspPI GGNCC 2 cut(s) 358, 373
PstI CTGCAG 2 cut(s) 608, 1293
PstNI CAGNNNCTG 1 cut(s) 603
PvuI CGATCG 1 cut(s) 589
PvuII CAGCTG 1 cut(s) 456
RruI TCGCGA 1 cut(s) 156
RsaI GTAC 5 cut(s) 269, 296, 542, 1232, 1269
RsaNI GTAC 5 cut(s) 268, 295, 541, 1231, 1268
RseI CAYNNNNRTG 1 cut(s) 1023
SapI GCTCTTC 1 cut(s) 777
SaqAI TTAA 2 cut(s) 666, 752
SatI GCNGC 6 cut(s) 7, 62, 532, 604, 1289, 1368
Sau3AI GATC 8 cut(s) 108, 345, 586, 844, 865, 1199, 1274, 1355
Sau96I GGNCC 2 cut(s) 358, 373
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 2 cut(s) 746, 1340
SduI GDGCHC 1 cut(s) 438
SfaNI GCATC 5 cut(s) 562, 659, 990, 997, 1139
SfcI CTRYAG 3 cut(s) 324, 604, 1289
SfuI TTCGAA 1 cut(s) 804
SinI GGWCC 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 1023
Sse9I AATT 7 cut(s) 203, 643, 678, 800, 904, 1112, 1205
SsiI CCGC 4 cut(s) 9, 185, 379, 968
SspMI CTAG 3 cut(s) 932, 1134, 1184
StyD4I CCNGG 2 cut(s) 744, 1338
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 2 cut(s) 22, 362
TaiI ACGT 1 cut(s) 430
TaqI TCGA 4 cut(s) 132, 589, 653, 804
TasI AATT 7 cut(s) 203, 643, 678, 800, 904, 1112, 1205
TatI WGTACW 3 cut(s) 294, 540, 1267
TfiI GAWTC 6 cut(s) 39, 88, 778, 953, 1162, 1211
Tru1I TTAA 2 cut(s) 666, 752
Tru9I TTAA 2 cut(s) 666, 752
TscAI CASTG 1 cut(s) 691
TseFI GTSAC 3 cut(s) 11, 68, 993
TseI GCWGC 6 cut(s) 6, 61, 531, 603, 1288, 1367
Tsp45I GTSAC 3 cut(s) 11, 68, 993
TspDTI ATGAA 7 cut(s) 455, 714, 813, 897, 953, 1041, 1203
TspRI CASTG 1 cut(s) 691
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 3 cut(s) 203, 800, 1205
XmnI GAANNNNTTC 2 cut(s) 206, 904
XspI CTAG 3 cut(s) 932, 1134, 1184
Zsp2I ATGCAT 1 cut(s) 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.