Rroxscaffold_1G00024950
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000652
Physical Location & Seq
Reverse (-)
31066112 .. 31068819
2708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00024950.1

Sequence Viewer

Length: 369 bp
ATGGGCAGAGGAATGTACAAGAACAAGCCCACTGGTCAGCGCCAGTTCTCCGGTCGCGAAGATCTTCTTGCCGGTACCTCCACCCGTCCCTGTTCTTTTTCAAAGGCATTTGATACCCTGCTCCGGAAGCTACAAAGGTATGTCCCTCTTGGAGCATCTATTGCTGATTGGTATGCAGGAGTAGGTGTTATCAGATTATCATTGGCTGCTACAAGAAAATGCAGGTCTGTGAGATGCATTGAGATTAACAAAGAATCAAAGCTATCCTTTGAGAAGACTGTTGCCCGCCTGCCAAACATAGTACATAGCAGCATCAGCTGGCATCATGCAGACACTTCTAAGATCCGCCTTCTTTTGACTATCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95 MTS

Protein Analysis

122

Amino Acids

13.67

Weight (kDa)

10.44

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000652)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18010 AT4G18010
fragaria_vesca FvH4_3g43741 FvH4_5g21310 FvH4_5g21310 FvH4_5g21310 FvH4_5g21310 FvH4_5g21310 FvH4_5g21310 FvH4_5g21310 FvH4_5g21310
malus_domestica MD06G1038900.v1.1 MD06G1039000.v1.1 MD06G1039100.v1.1
prunus_persica Prupe.5G044100_v2.0.a1 Prupe.5G044100_v2.0.a1 Prupe.5G044100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0366181 RchiOBHm_Chr3g0482901 RchiOBHm_Chr3g0496141 RchiOBHm_Chr4g0424971 RchiOBHm_Chr5g0027221 RchiOBHm_Chr5g0059741 RchiOBHm_Chr7g0179331 RchiOBHm_Chr7g0187161 RchiOBHm_Chr7g0208651
rosa_laevigata RLG00000003205
rosa_multiflora Rmu_co8056148.1_g000001 Rmu_sc0000072.1_g000010 Rmu_sc0001758.1_g000030 Rmu_sc0003032.1_g000022 Rmu_sc0005406.1_g000008 Rmu_sc0008717.1_g000001 Rmu_sc0013772.1_g000009 Rmu_sc0025894.1_g000001
rosa_roxburghii Rroxscaffold_1G00001950 Rroxscaffold_1G00024950 Rroxscaffold_3G00250340 Rroxscaffold_3G00250350 Rroxscaffold_7G00169250 Rroxscaffold_7G00182410
rosa_rugosa Rorug05G0102000 Rorug05G0153100 Rorug05G0306800 Rorug05G0360900 Rorug06G0112500 Rorug07G0105200 Rorug07G0105200
rosa_samantha Rh1BG155200 Rh1CG036100 Rh1CG073400 Rh1DG080300 Rh1DG187100 Rh2BG453300 Rh3AG250800 Rh3AG258400 Rh3AG326800 Rh3DG236500 Rh4DG084600 Rh5BG330300 Rh5BG531100 Rh5CG555400 Rh5DG341500 Rh6AG492200 Rh6CG362800 Rh6DG217600 Rh7AG084500 Rh7AG163600 Rh7AG240200 Rh7AG467900 Rh7AG482100 Rh7BG235300 Rh7CG254900 Rh7DG086600 Rh7DG246200 Rh7DG333900
rosa_wichuraiana Rw7G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 213
Acc65I GGTACC 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 74
AccII CGCG 1 cut(s) 57
AccIII TCCGGA 1 cut(s) 123
AciI CCGC 2 cut(s) 286, 346
AclWI GGATC 1 cut(s) 337
AfaI GTAC 3 cut(s) 17, 76, 303
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 1 cut(s) 102
AluBI AGCT 3 cut(s) 130, 262, 318
AluI AGCT 3 cut(s) 130, 262, 318
AlwI GGATC 1 cut(s) 337
Aor13HI TCCGGA 1 cut(s) 123
ApeKI GCWGC 2 cut(s) 206, 309
Asp700I GAANNNNTTC 1 cut(s) 63
Asp718I GGTACC 1 cut(s) 74
AspLEI GCGC 1 cut(s) 42
BanI GGYRCC 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 281
BbvI GCAGC 2 cut(s) 193, 321
BfoI RGCGCY 1 cut(s) 43
BfuAI ACCTGC 1 cut(s) 213
BglII AGATCT 1 cut(s) 61
BisI GCNGC 2 cut(s) 207, 310
BlsI GCNGC 2 cut(s) 208, 311
BmiI GGNNCC 1 cut(s) 76
BmsI GCATC 4 cut(s) 164, 224, 321, 331
BpiI GAAGAC 1 cut(s) 281
BsaWI WCCGGW 2 cut(s) 50, 123
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 2 cut(s) 37, 43
BseAI TCCGGA 1 cut(s) 123
BseLI CCNNNNNNNGG 1 cut(s) 123
BseNI ACTGG 2 cut(s) 37, 43
BseXI GCAGC 2 cut(s) 193, 321
Bsh1236I CGCG 1 cut(s) 57
Bsh1285I CGRYCG 1 cut(s) 55
BshNI GGYRCC 1 cut(s) 74
BsiEI CGRYCG 1 cut(s) 55
BsiSI CCGG 3 cut(s) 51, 72, 124
BslFI GGGAC 2 cut(s) 72, 128
BslI CCNNNNNNNGG 1 cut(s) 123
BsmFI GGGAC 2 cut(s) 72, 128
Bsp13I TCCGGA 1 cut(s) 123
Bsp1407I TGTACA 1 cut(s) 15
Bsp143I GATC 2 cut(s) 61, 342
Bsp68I TCGCGA 1 cut(s) 57
BspACI CCGC 2 cut(s) 286, 346
BspEI TCCGGA 1 cut(s) 123
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 76
BspMI ACCTGC 1 cut(s) 213
BspPI GGATC 1 cut(s) 337
BspT107I GGYRCC 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 71
BsrGI TGTACA 1 cut(s) 15
BsrI ACTGG 2 cut(s) 37, 43
BssAI RCCGGY 1 cut(s) 71
BssMI GATC 2 cut(s) 61, 342
Bst4CI ACNGT 1 cut(s) 280
BstAPI GCANNNNNTGC 1 cut(s) 161
BstAUI TGTACA 1 cut(s) 15
BstC8I GCNNGC 3 cut(s) 286, 290, 320
BstDEI CTNAG 1 cut(s) 339
BstFNI CGCG 1 cut(s) 57
BstH2I RGCGCY 1 cut(s) 43
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 2 cut(s) 64, 345
BstMBI GATC 2 cut(s) 61, 342
BstMCI CGRYCG 1 cut(s) 55
BstMWI GCNNNNNNNGC 3 cut(s) 127, 161, 315
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 2 cut(s) 193, 321
BstV2I GAAGAC 1 cut(s) 281
BstX2I RGATCY 2 cut(s) 61, 342
BstYI RGATCY 2 cut(s) 61, 342
BtsIMutI CAGTG 1 cut(s) 30
BtuMI TCGCGA 1 cut(s) 57
BveI ACCTGC 1 cut(s) 213
Cac8I GCNNGC 3 cut(s) 286, 290, 320
CfoI GCGC 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 71
Csp6I GTAC 3 cut(s) 16, 75, 302
CviAII CATG 1 cut(s) 326
CviJI RGCY 5 cut(s) 28, 130, 206, 262, 318
CviKI_1 RGCY 5 cut(s) 28, 130, 206, 262, 318
CviQI GTAC 3 cut(s) 16, 75, 302
DdeI CTNAG 1 cut(s) 339
DpnI GATC 2 cut(s) 63, 344
DpnII GATC 2 cut(s) 61, 342
EciI GGCGGA 1 cut(s) 335
EcoT22I ATGCAT 1 cut(s) 239
FaeI CATG 1 cut(s) 329
FaiI YATR 5 cut(s) 141, 174, 299, 306, 327
FalI AAGNNNNNCTT 4 cut(s) 51, 83, 251, 283
FaqI GGGAC 2 cut(s) 72, 128
FatI CATG 1 cut(s) 325
FauI CCCGC 1 cut(s) 293
Fnu4HI GCNGC 2 cut(s) 207, 310
Fsp4HI GCNGC 2 cut(s) 207, 310
GlaI GCGC 1 cut(s) 41
GluI GCNGC 2 cut(s) 207, 310
HaeII RGCGCY 1 cut(s) 43
HapII CCGG 3 cut(s) 51, 72, 124
HhaI GCGC 1 cut(s) 42
Hin1II CATG 1 cut(s) 329
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HinfI GANTC 1 cut(s) 254
HpaII CCGG 3 cut(s) 51, 72, 124
Hpy188I TCNGA 1 cut(s) 194
Hpy188III TCNNGA 2 cut(s) 56, 124
HpyAV CCTTC 1 cut(s) 359
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4V TGCA 4 cut(s) 176, 222, 237, 329
HpyF10VI GCNNNNNNNGC 3 cut(s) 127, 161, 315
HpyF3I CTNAG 1 cut(s) 339
Hsp92II CATG 1 cut(s) 329
HspAI GCGC 1 cut(s) 40
Kpn2I TCCGGA 1 cut(s) 123
KpnI GGTACC 1 cut(s) 78
Kzo9I GATC 2 cut(s) 61, 342
LmnI GCTCC 2 cut(s) 126, 152
Lsp1109I GCAGC 2 cut(s) 193, 321
LweI GCATC 4 cut(s) 164, 224, 321, 331
MalI GATC 2 cut(s) 63, 344
MboI GATC 2 cut(s) 61, 342
MboII GAAGA 3 cut(s) 56, 71, 286
MflI RGATCY 2 cut(s) 61, 342
MnlI CCTC 2 cut(s) 88, 156
Mph1103I ATGCAT 1 cut(s) 239
MroI TCCGGA 1 cut(s) 123
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 2 cut(s) 246, 367
MspA1I CMGCKG 1 cut(s) 318
MspI CCGG 3 cut(s) 51, 72, 124
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 3 cut(s) 127, 161, 315
NdeII GATC 2 cut(s) 61, 342
NlaIII CATG 1 cut(s) 329
NlaIV GGNNCC 1 cut(s) 76
NruI TCGCGA 1 cut(s) 57
NsiI ATGCAT 1 cut(s) 239
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 1 cut(s) 254
PkrI GCNGC 2 cut(s) 208, 311
PspN4I GGNNCC 1 cut(s) 76
PsuI RGATCY 2 cut(s) 61, 342
PvuII CAGCTG 1 cut(s) 318
RruI TCGCGA 1 cut(s) 57
RsaI GTAC 3 cut(s) 17, 76, 303
RsaNI GTAC 3 cut(s) 16, 75, 302
SaqAI TTAA 2 cut(s) 246, 367
SatI GCNGC 2 cut(s) 207, 310
Sau3AI GATC 2 cut(s) 61, 342
SetI ASST 7 cut(s) 80, 132, 140, 187, 227, 264, 320
SfaNI GCATC 4 cut(s) 164, 224, 321, 331
SsiI CCGC 2 cut(s) 286, 346
TaaI ACNGT 1 cut(s) 280
TatI WGTACW 2 cut(s) 15, 301
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 2 cut(s) 246, 367
Tru9I TTAA 2 cut(s) 246, 367
TscAI CASTG 1 cut(s) 37
TseI GCWGC 2 cut(s) 206, 309
TspRI CASTG 1 cut(s) 37
XmnI GAANNNNTTC 1 cut(s) 63
Zsp2I ATGCAT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.