Rh2BG453300
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000652
Physical Location & Seq
Forward (+)
64032615 .. 64036646
4032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG453300.1

Sequence Viewer

Length: 399 bp
ATGTTGGTGGGCGGCATGAAGGTGGTTGGGATTTACATTTGGATTAGCGATAATGCATTCAAGAATTCAAACATTGTGCTTTGCCAGGCATTTGATACCCTGCTCCGGAAGCTACAAAGGTATGTCCCTCTTGGAGCATCTGTTGCTGATTGGTATGCAGGAGTAGGTGTTATCAGATTATCATTGGCTGCTACAAGAAAATGCAGGTCTGTGAGATGCATTGAGATTAACAAAGAATCAAAGCTATCCTTTGAGAAGACGGTTGCCCGCCTGCCAAACATAGTACATAGCAGCATCAGCTGGCATCATGCAGACACTTCTAAGGTGAAGGAATGCACGTCAGCCTTGCTGCGTTTTGCGAAGAGGCCTCATTTGAGCGAGCAAAAAAATGGGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95 MTS

Protein Analysis

132

Amino Acids

14.76

Weight (kDa)

9.82

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000652)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18010 AT4G18010
fragaria_vesca FvH4_3g43741 FvH4_5g21310 FvH4_5g21310 FvH4_5g21310 FvH4_5g21310 FvH4_5g21310 FvH4_5g21310 FvH4_5g21310 FvH4_5g21310
malus_domestica MD06G1038900.v1.1 MD06G1039000.v1.1 MD06G1039100.v1.1
prunus_persica Prupe.5G044100_v2.0.a1 Prupe.5G044100_v2.0.a1 Prupe.5G044100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0366181 RchiOBHm_Chr3g0482901 RchiOBHm_Chr3g0496141 RchiOBHm_Chr4g0424971 RchiOBHm_Chr5g0027221 RchiOBHm_Chr5g0059741 RchiOBHm_Chr7g0179331 RchiOBHm_Chr7g0187161 RchiOBHm_Chr7g0208651
rosa_laevigata RLG00000003205
rosa_multiflora Rmu_co8056148.1_g000001 Rmu_sc0000072.1_g000010 Rmu_sc0001758.1_g000030 Rmu_sc0003032.1_g000022 Rmu_sc0005406.1_g000008 Rmu_sc0008717.1_g000001 Rmu_sc0013772.1_g000009 Rmu_sc0025894.1_g000001
rosa_roxburghii Rroxscaffold_1G00001950 Rroxscaffold_1G00024950 Rroxscaffold_3G00250340 Rroxscaffold_3G00250350 Rroxscaffold_7G00169250 Rroxscaffold_7G00182410
rosa_rugosa Rorug05G0102000 Rorug05G0153100 Rorug05G0306800 Rorug05G0360900 Rorug06G0112500 Rorug07G0105200 Rorug07G0105200
rosa_samantha Rh1BG155200 Rh1CG036100 Rh1CG073400 Rh1DG080300 Rh1DG187100 Rh2BG453300 Rh3AG250800 Rh3AG258400 Rh3AG326800 Rh3DG236500 Rh4DG084600 Rh5BG330300 Rh5BG531100 Rh5CG555400 Rh5DG341500 Rh6AG492200 Rh6CG362800 Rh6DG217600 Rh7AG084500 Rh7AG163600 Rh7AG240200 Rh7AG467900 Rh7AG482100 Rh7BG235300 Rh7CG254900 Rh7DG086600 Rh7DG246200 Rh7DG333900
rosa_wichuraiana Rw7G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 195
AccIII TCCGGA 1 cut(s) 105
AciI CCGC 2 cut(s) 12, 268
AcsI RAATTY 1 cut(s) 64
AfaI GTAC 1 cut(s) 285
AfiI CCNNNNNNNGG 1 cut(s) 105
AgsI TTSAA 2 cut(s) 61, 69
AjiI CACGTC 1 cut(s) 339
AjnI CCWGG 1 cut(s) 84
AluBI AGCT 3 cut(s) 112, 244, 300
AluI AGCT 3 cut(s) 112, 244, 300
Aor13HI TCCGGA 1 cut(s) 105
AoxI GGCC 1 cut(s) 365
ApeKI GCWGC 3 cut(s) 188, 291, 349
ApoI RAATTY 1 cut(s) 64
AsuHPI GGTGA 1 cut(s) 337
BbsI GAAGAC 1 cut(s) 263
BbvI GCAGC 3 cut(s) 175, 303, 336
BciT130I CCWGG 1 cut(s) 86
BfaI CTAG 1 cut(s) 397
BfuAI ACCTGC 1 cut(s) 195
BisI GCNGC 4 cut(s) 13, 189, 292, 350
BlsI GCNGC 4 cut(s) 14, 190, 293, 351
Bme1390I CCNGG 1 cut(s) 86
BmgBI CACGTC 1 cut(s) 339
BmrFI CCNGG 1 cut(s) 86
BmsI GCATC 4 cut(s) 146, 206, 303, 313
BpiI GAAGAC 1 cut(s) 263
BsaWI WCCGGW 1 cut(s) 105
BsaXI ACNNNNNCTCC 4 cut(s) 126, 153, 156, 183
Bsc4I CCNNNNNNNGG 1 cut(s) 105
BseAI TCCGGA 1 cut(s) 105
BseBI CCWGG 1 cut(s) 86
BseLI CCNNNNNNNGG 1 cut(s) 105
BseXI GCAGC 3 cut(s) 175, 303, 336
BshFI GGCC 1 cut(s) 367
BsiSI CCGG 1 cut(s) 106
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 110
BsmI GAATGC 2 cut(s) 56, 338
BsnI GGCC 1 cut(s) 367
Bsp13I TCCGGA 1 cut(s) 105
BspACI CCGC 2 cut(s) 12, 268
BspANI GGCC 1 cut(s) 367
BspEI TCCGGA 1 cut(s) 105
BspMI ACCTGC 1 cut(s) 195
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 1 cut(s) 262
Bst6I CTCTTC 1 cut(s) 356
BstAPI GCANNNNNTGC 1 cut(s) 143
BstC8I GCNNGC 4 cut(s) 268, 272, 302, 380
BstDEI CTNAG 1 cut(s) 321
BstMWI GCNNNNNNNGC 3 cut(s) 109, 143, 297
BstNI CCWGG 1 cut(s) 86
BstSCI CCNGG 1 cut(s) 84
BstV1I GCAGC 3 cut(s) 175, 303, 336
BstV2I GAAGAC 1 cut(s) 263
BsuRI GGCC 1 cut(s) 367
BtrI CACGTC 1 cut(s) 339
BveI ACCTGC 1 cut(s) 195
Cac8I GCNNGC 4 cut(s) 268, 272, 302, 380
Csp6I GTAC 1 cut(s) 284
CviAII CATG 2 cut(s) 16, 308
CviJI RGCY 6 cut(s) 112, 188, 244, 300, 344, 367
CviKI_1 RGCY 6 cut(s) 112, 188, 244, 300, 344, 367
CviQI GTAC 1 cut(s) 284
DdeI CTNAG 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
Eco147I AGGCCT 1 cut(s) 367
EcoRI GAATTC 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 84
EcoT22I ATGCAT 2 cut(s) 58, 221
FaeI CATG 2 cut(s) 19, 311
FaiI YATR 6 cut(s) 17, 123, 156, 281, 288, 309
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 1 cut(s) 110
FatI CATG 2 cut(s) 15, 307
FauI CCCGC 1 cut(s) 275
Fnu4HI GCNGC 4 cut(s) 13, 189, 292, 350
Fsp4HI GCNGC 4 cut(s) 13, 189, 292, 350
FspBI CTAG 1 cut(s) 397
GluI GCNGC 4 cut(s) 13, 189, 292, 350
HaeIII GGCC 1 cut(s) 367
HapII CCGG 1 cut(s) 106
Hin1II CATG 2 cut(s) 19, 311
HinfI GANTC 1 cut(s) 236
HpaII CCGG 1 cut(s) 106
HphI GGTGA 1 cut(s) 337
Hpy188I TCNGA 1 cut(s) 176
Hpy188III TCNNGA 2 cut(s) 61, 106
HpyAV CCTTC 2 cut(s) 13, 322
HpyCH4III ACNGT 1 cut(s) 262
HpyCH4IV ACGT 1 cut(s) 338
HpyCH4V TGCA 6 cut(s) 56, 158, 204, 219, 311, 336
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 143, 297
HpyF3I CTNAG 1 cut(s) 321
HpySE526I ACGT 1 cut(s) 338
Hsp92II CATG 2 cut(s) 19, 311
Kpn2I TCCGGA 1 cut(s) 105
LmnI GCTCC 2 cut(s) 108, 134
LpnPI CCDG 8 cut(s) 71, 98, 113, 119, 144, 190, 284, 286
Lsp1109I GCAGC 3 cut(s) 175, 303, 336
LweI GCATC 4 cut(s) 146, 206, 303, 313
MaeI CTAG 1 cut(s) 397
MaeII ACGT 1 cut(s) 338
MboII GAAGA 2 cut(s) 268, 373
MluCI AATT 1 cut(s) 64
MnlI CCTC 3 cut(s) 138, 357, 378
Mph1103I ATGCAT 2 cut(s) 58, 221
MroI TCCGGA 1 cut(s) 105
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 20
MspA1I CMGCKG 1 cut(s) 300
MspI CCGG 1 cut(s) 106
MspR9I CCNGG 1 cut(s) 86
Mva1269I GAATGC 2 cut(s) 56, 338
MvaI CCWGG 1 cut(s) 86
MwoI GCNNNNNNNGC 3 cut(s) 109, 143, 297
NlaIII CATG 2 cut(s) 19, 311
NsiI ATGCAT 2 cut(s) 58, 221
PceI AGGCCT 1 cut(s) 367
PctI GAATGC 2 cut(s) 56, 338
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 4 cut(s) 14, 190, 293, 351
Psp6I CCWGG 1 cut(s) 84
PspGI CCWGG 1 cut(s) 84
PvuII CAGCTG 1 cut(s) 300
RsaI GTAC 1 cut(s) 285
RsaNI GTAC 1 cut(s) 284
RseI CAYNNNNRTG 1 cut(s) 20
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 4 cut(s) 13, 189, 292, 350
ScrFI CCNGG 1 cut(s) 86
SetI ASST 9 cut(s) 24, 114, 122, 169, 209, 246, 302, 327, 341
SfaNI GCATC 4 cut(s) 146, 206, 303, 313
SmiMI CAYNNNNRTG 1 cut(s) 20
Sse9I AATT 1 cut(s) 64
SseBI AGGCCT 1 cut(s) 367
SsiI CCGC 2 cut(s) 12, 268
SspMI CTAG 1 cut(s) 397
StuI AGGCCT 1 cut(s) 367
StyD4I CCNGG 1 cut(s) 84
TaaI ACNGT 1 cut(s) 262
TaiI ACGT 1 cut(s) 341
TasI AATT 1 cut(s) 64
TatI WGTACW 1 cut(s) 283
TauI GCSGC 1 cut(s) 15
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseI GCWGC 3 cut(s) 188, 291, 349
TspDTI ATGAA 1 cut(s) 32
XapI RAATTY 1 cut(s) 64
XspI CTAG 1 cut(s) 397
Zsp2I ATGCAT 2 cut(s) 58, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.