Belongs to the glycosyl hydrolase 18 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 590 |
| AccII | CGCG | 1 cut(s) | 99 |
| AciI | CCGC | 1 cut(s) | 640 |
| AclWI | GGATC | 1 cut(s) | 413 |
| AcsI | RAATTY | 2 cut(s) | 272, 380 |
| AfaI | GTAC | 1 cut(s) | 426 |
| AflII | CTTAAG | 1 cut(s) | 662 |
| AflIII | ACRYGT | 1 cut(s) | 58 |
| AgeI | ACCGGT | 1 cut(s) | 578 |
| AgsI | TTSAA | 3 cut(s) | 146, 163, 313 |
| AjnI | CCWGG | 1 cut(s) | 81 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 230, 262 |
| AluBI | AGCT | 4 cut(s) | 404, 493, 670, 674 |
| AluI | AGCT | 4 cut(s) | 404, 493, 670, 674 |
| Alw26I | GTCTC | 1 cut(s) | 608 |
| AlwI | GGATC | 1 cut(s) | 413 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 488 |
| AoxI | GGCC | 3 cut(s) | 84, 117, 203 |
| ApeKI | GCWGC | 2 cut(s) | 493, 509 |
| ApoI | RAATTY | 2 cut(s) | 272, 380 |
| AsiGI | ACCGGT | 1 cut(s) | 578 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 158, 317 |
| AspLEI | GCGC | 2 cut(s) | 101, 151 |
| AspS9I | GGNCC | 2 cut(s) | 117, 435 |
| AsuHPI | GGTGA | 1 cut(s) | 313 |
| AsuNHI | GCTAGC | 1 cut(s) | 670 |
| AvaII | GGWCC | 1 cut(s) | 435 |
| BaeGI | GKGCMC | 1 cut(s) | 302 |
| BbvI | GCAGC | 2 cut(s) | 480, 521 |
| BccI | CCATC | 3 cut(s) | 323, 331, 341 |
| BciT130I | CCWGG | 1 cut(s) | 83 |
| BciVI | GTATCC | 1 cut(s) | 244 |
| BcoDI | GTCTC | 1 cut(s) | 608 |
| BfaI | CTAG | 1 cut(s) | 671 |
| BfmI | CTRYAG | 2 cut(s) | 195, 750 |
| BfrI | CTTAAG | 1 cut(s) | 662 |
| BfuAI | ACCTGC | 1 cut(s) | 590 |
| BfuI | GTATCC | 1 cut(s) | 244 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 92 |
| BisI | GCNGC | 2 cut(s) | 494, 510 |
| BlpI | GCTNAGC | 1 cut(s) | 489 |
| BlsI | GCNGC | 2 cut(s) | 495, 511 |
| BmcAI | AGTACT | 1 cut(s) | 426 |
| Bme1390I | CCNGG | 1 cut(s) | 83 |
| Bme18I | GGWCC | 1 cut(s) | 435 |
| BmgT120I | GGNCC | 2 cut(s) | 117, 435 |
| BmiI | GGNNCC | 1 cut(s) | 118 |
| BmrFI | CCNGG | 1 cut(s) | 83 |
| BmrI | ACTGGG | 2 cut(s) | 352, 564 |
| BmsI | GCATC | 1 cut(s) | 535 |
| BmtI | GCTAGC | 1 cut(s) | 674 |
| BmuI | ACTGGG | 2 cut(s) | 352, 564 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 489 |
| BsaI | GGTCTC | 1 cut(s) | 608 |
| BsaJI | CCNNGG | 2 cut(s) | 81, 449 |
| BsaWI | WCCGGW | 1 cut(s) | 578 |
| Bse118I | RCCGGY | 2 cut(s) | 91, 578 |
| Bse1I | ACTGG | 4 cut(s) | 347, 422, 559, 766 |
| Bse3DI | GCAATG | 4 cut(s) | 9, 26, 519, 707 |
| BseBI | CCWGG | 1 cut(s) | 83 |
| BseDI | CCNNGG | 2 cut(s) | 81, 449 |
| BseGI | GGATG | 2 cut(s) | 352, 394 |
| BseMI | GCAATG | 4 cut(s) | 9, 26, 519, 707 |
| BseMII | CTCAG | 1 cut(s) | 480 |
| BseNI | ACTGG | 4 cut(s) | 347, 422, 559, 766 |
| BseRI | GAGGAG | 1 cut(s) | 388 |
| BseSI | GKGCMC | 1 cut(s) | 302 |
| BseXI | GCAGC | 2 cut(s) | 480, 521 |
| Bsh1236I | CGCG | 1 cut(s) | 99 |
| Bsh1285I | CGRYCG | 1 cut(s) | 505 |
| BshFI | GGCC | 3 cut(s) | 86, 119, 205 |
| BshTI | ACCGGT | 1 cut(s) | 578 |
| BsiEI | CGRYCG | 1 cut(s) | 505 |
| BsiSI | CCGG | 2 cut(s) | 92, 579 |
| BslFI | GGGAC | 1 cut(s) | 121 |
| BsmAI | GTCTC | 1 cut(s) | 608 |
| BsmFI | GGGAC | 1 cut(s) | 121 |
| BsnI | GGCC | 3 cut(s) | 86, 119, 205 |
| Bso31I | GGTCTC | 1 cut(s) | 608 |
| Bsp1286I | GDGCHC | 1 cut(s) | 302 |
| Bsp143I | GATC | 1 cut(s) | 418 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 489 |
| BspACI | CCGC | 1 cut(s) | 640 |
| BspANI | GGCC | 3 cut(s) | 86, 119, 205 |
| BspCNI | CTCAG | 1 cut(s) | 481 |
| BspFNI | CGCG | 1 cut(s) | 99 |
| BspLI | GGNNCC | 1 cut(s) | 118 |
| BspMI | ACCTGC | 1 cut(s) | 590 |
| BspOI | GCTAGC | 1 cut(s) | 674 |
| BspPI | GGATC | 1 cut(s) | 413 |
| BspTI | CTTAAG | 1 cut(s) | 662 |
| BspTNI | GGTCTC | 1 cut(s) | 608 |
| BsrDI | GCAATG | 4 cut(s) | 9, 26, 519, 707 |
| BsrFI | RCCGGY | 2 cut(s) | 91, 578 |
| BsrI | ACTGG | 4 cut(s) | 347, 422, 559, 766 |
| BssAI | RCCGGY | 2 cut(s) | 91, 578 |
| BssECI | CCNNGG | 2 cut(s) | 81, 449 |
| BssMI | GATC | 1 cut(s) | 418 |
| BssT1I | CCWWGG | 1 cut(s) | 449 |
| Bst2UI | CCWGG | 1 cut(s) | 83 |
| Bst4CI | ACNGT | 4 cut(s) | 429, 503, 616, 633 |
| BstAFI | CTTAAG | 1 cut(s) | 662 |
| BstC8I | GCNNGC | 3 cut(s) | 97, 243, 672 |
| BstDEI | CTNAG | 1 cut(s) | 489 |
| BstF5I | GGATG | 2 cut(s) | 352, 394 |
| BstFNI | CGCG | 1 cut(s) | 99 |
| BstHHI | GCGC | 2 cut(s) | 101, 151 |
| BstKTI | GATC | 1 cut(s) | 421 |
| BstMAI | GTCTC | 1 cut(s) | 608 |
| BstMBI | GATC | 1 cut(s) | 418 |
| BstMCI | CGRYCG | 1 cut(s) | 505 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 11, 92, 680 |
| BstNI | CCWGG | 1 cut(s) | 83 |
| BstNSI | RCATGY | 1 cut(s) | 62 |
| BstSCI | CCNGG | 1 cut(s) | 81 |
| BstSFI | CTRYAG | 2 cut(s) | 195, 750 |
| BstSLI | GKGCMC | 1 cut(s) | 302 |
| BstUI | CGCG | 1 cut(s) | 99 |
| BstV1I | GCAGC | 2 cut(s) | 480, 521 |
| BsuI | GTATCC | 1 cut(s) | 244 |
| BsuRI | GGCC | 3 cut(s) | 86, 119, 205 |
| BtsCI | GGATG | 2 cut(s) | 352, 394 |
| BtsIMutI | CAGTG | 2 cut(s) | 283, 621 |
| BveI | ACCTGC | 1 cut(s) | 590 |
| Cac8I | GCNNGC | 3 cut(s) | 97, 243, 672 |
| CaiI | CAGNNNCTG | 1 cut(s) | 488 |
| CfoI | GCGC | 2 cut(s) | 101, 151 |
| Cfr10I | RCCGGY | 2 cut(s) | 91, 578 |
| Cfr13I | GGNCC | 2 cut(s) | 117, 435 |
| CseI | GACGC | 1 cut(s) | 218 |
| Csp6I | GTAC | 1 cut(s) | 425 |
| CspAI | ACCGGT | 1 cut(s) | 578 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 109, 144 |
| CviAII | CATG | 4 cut(s) | 59, 303, 459, 529 |
| CviQI | GTAC | 1 cut(s) | 425 |
| DdeI | CTNAG | 1 cut(s) | 489 |
| DpnI | GATC | 1 cut(s) | 420 |
| DpnII | GATC | 1 cut(s) | 418 |
| EciI | GGCGGA | 1 cut(s) | 655 |
| Eco130I | CCWWGG | 1 cut(s) | 449 |
| Eco31I | GGTCTC | 1 cut(s) | 608 |
| Eco47I | GGWCC | 1 cut(s) | 435 |
| EcoO109I | RGGNCCY | 1 cut(s) | 435 |
| EcoRII | CCWGG | 1 cut(s) | 81 |
| EcoT14I | CCWWGG | 1 cut(s) | 449 |
| ErhI | CCWWGG | 1 cut(s) | 449 |
| FaeI | CATG | 4 cut(s) | 62, 306, 462, 532 |
| FaqI | GGGAC | 1 cut(s) | 121 |
| FatI | CATG | 4 cut(s) | 58, 302, 458, 528 |
| FauNDI | CATATG | 2 cut(s) | 535, 590 |
| Fnu4HI | GCNGC | 2 cut(s) | 494, 510 |
| FokI | GGATG | 2 cut(s) | 359, 381 |
| Fsp4HI | GCNGC | 2 cut(s) | 494, 510 |
| FspBI | CTAG | 1 cut(s) | 671 |
| GlaI | GCGC | 2 cut(s) | 100, 150 |
| GluI | GCNGC | 2 cut(s) | 494, 510 |
| HaeIII | GGCC | 3 cut(s) | 86, 119, 205 |
| HapII | CCGG | 2 cut(s) | 92, 579 |
| HgaI | GACGC | 1 cut(s) | 218 |
| HhaI | GCGC | 2 cut(s) | 101, 151 |
| Hin1II | CATG | 4 cut(s) | 62, 306, 462, 532 |
| Hin6I | GCGC | 2 cut(s) | 99, 149 |
| HinP1I | GCGC | 2 cut(s) | 99, 149 |
| HindIII | AAGCTT | 1 cut(s) | 402 |
| HinfI | GANTC | 1 cut(s) | 257 |
| HpaII | CCGG | 2 cut(s) | 92, 579 |
| HphI | GGTGA | 1 cut(s) | 313 |
| Hpy166II | GTNNAC | 3 cut(s) | 53, 186, 321 |
| Hpy188I | TCNGA | 1 cut(s) | 572 |
| Hpy188III | TCNNGA | 2 cut(s) | 386, 398 |
| Hpy8I | GTNNAC | 3 cut(s) | 53, 186, 321 |
| Hpy99I | CGWCG | 1 cut(s) | 419 |
| HpyAV | CCTTC | 1 cut(s) | 289 |
| HpyCH4III | ACNGT | 4 cut(s) | 429, 503, 616, 633 |
| HpyCH4V | TGCA | 3 cut(s) | 31, 594, 737 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 11, 92, 680 |
| HpyF3I | CTNAG | 1 cut(s) | 489 |
| Hsp92II | CATG | 4 cut(s) | 62, 306, 462, 532 |
| HspAI | GCGC | 2 cut(s) | 99, 149 |
| Kzo9I | GATC | 1 cut(s) | 418 |
| Lsp1109I | GCAGC | 2 cut(s) | 480, 521 |
| LweI | GCATC | 1 cut(s) | 535 |
| MaeI | CTAG | 1 cut(s) | 671 |
| MaeIII | GTNAC | 2 cut(s) | 61, 581 |
| MalI | GATC | 1 cut(s) | 420 |
| MboI | GATC | 1 cut(s) | 418 |
| MboII | GAAGA | 7 cut(s) | 12, 15, 252, 304, 517, 659, 702 |
| MhlI | GDGCHC | 1 cut(s) | 302 |
| MluCI | AATT | 3 cut(s) | 164, 272, 380 |
| MmeI | TCCRAC | 1 cut(s) | 550 |
| MnlI | CCTC | 8 cut(s) | 208, 279, 366, 369, 448, 452, 478, 673 |
| MroXI | GAANNNNTTC | 2 cut(s) | 158, 317 |
| MseI | TTAA | 2 cut(s) | 663, 775 |
| MspCI | CTTAAG | 1 cut(s) | 662 |
| MspI | CCGG | 2 cut(s) | 92, 579 |
| MspR9I | CCNGG | 1 cut(s) | 83 |
| MvaI | CCWGG | 1 cut(s) | 83 |
| MvnI | CGCG | 1 cut(s) | 99 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 11, 92, 680 |
| NdeI | CATATG | 2 cut(s) | 535, 590 |
| NdeII | GATC | 1 cut(s) | 418 |
| NheI | GCTAGC | 1 cut(s) | 670 |
| NlaIII | CATG | 4 cut(s) | 62, 306, 462, 532 |
| NlaIV | GGNNCC | 1 cut(s) | 118 |
| NspI | RCATGY | 1 cut(s) | 62 |
| PciI | ACATGT | 1 cut(s) | 58 |
| PdmI | GAANNNNTTC | 2 cut(s) | 158, 317 |
| PfeI | GAWTC | 1 cut(s) | 257 |
| PinAI | ACCGGT | 1 cut(s) | 578 |
| PkrI | GCNGC | 2 cut(s) | 495, 511 |
| PpuMI | RGGWCCY | 1 cut(s) | 435 |
| PscI | ACATGT | 1 cut(s) | 58 |
| Psp5II | RGGWCCY | 1 cut(s) | 435 |
| Psp6I | CCWGG | 1 cut(s) | 81 |
| PspGI | CCWGG | 1 cut(s) | 81 |
| PspN4I | GGNNCC | 1 cut(s) | 118 |
| PspPI | GGNCC | 2 cut(s) | 117, 435 |
| PspPPI | RGGWCCY | 1 cut(s) | 435 |
| PstNI | CAGNNNCTG | 1 cut(s) | 488 |
| RsaI | GTAC | 1 cut(s) | 426 |
| RsaNI | GTAC | 1 cut(s) | 425 |
| SaqAI | TTAA | 2 cut(s) | 663, 775 |
| SatI | GCNGC | 2 cut(s) | 494, 510 |
| Sau3AI | GATC | 1 cut(s) | 418 |
| Sau96I | GGNCC | 2 cut(s) | 117, 435 |
| ScaI | AGTACT | 1 cut(s) | 426 |
| ScrFI | CCNGG | 1 cut(s) | 83 |
| SduI | GDGCHC | 1 cut(s) | 302 |
| SetI | ASST | 8 cut(s) | 290, 406, 440, 495, 604, 663, 672, 676 |
| SfaNI | GCATC | 1 cut(s) | 535 |
| SfcI | CTRYAG | 2 cut(s) | 195, 750 |
| SinI | GGWCC | 1 cut(s) | 435 |
| SmlI | CTYRAG | 1 cut(s) | 662 |
| SmoI | CTYRAG | 1 cut(s) | 662 |
| Sse9I | AATT | 3 cut(s) | 164, 272, 380 |
| SsiI | CCGC | 1 cut(s) | 640 |
| SspMI | CTAG | 1 cut(s) | 671 |
| StyD4I | CCNGG | 1 cut(s) | 81 |
| StyI | CCWWGG | 1 cut(s) | 449 |
| TaaI | ACNGT | 4 cut(s) | 429, 503, 616, 633 |
| TaqI | TCGA | 4 cut(s) | 236, 387, 505, 654 |
| TasI | AATT | 3 cut(s) | 164, 272, 380 |
| TatI | WGTACW | 1 cut(s) | 424 |
| TfiI | GAWTC | 1 cut(s) | 257 |
| Tru1I | TTAA | 2 cut(s) | 663, 775 |
| Tru9I | TTAA | 2 cut(s) | 663, 775 |
| TscAI | CASTG | 2 cut(s) | 283, 621 |
| TseI | GCWGC | 2 cut(s) | 493, 509 |
| TspDTI | ATGAA | 4 cut(s) | 15, 383, 517, 577 |
| TspGWI | ACGGA | 1 cut(s) | 659 |
| TspRI | CASTG | 2 cut(s) | 283, 621 |
| Vha464I | CTTAAG | 1 cut(s) | 662 |
| VpaK11BI | GGWCC | 1 cut(s) | 435 |
| XapI | RAATTY | 2 cut(s) | 272, 380 |
| XceI | RCATGY | 1 cut(s) | 62 |
| XmnI | GAANNNNTTC | 2 cut(s) | 158, 317 |
| XspI | CTAG | 1 cut(s) | 671 |
| ZrmI | AGTACT | 1 cut(s) | 426 |