Rh1CG257400

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000601
Physical Location & Seq
Forward (+)
52170009 .. 52170830
822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG257400.1

Sequence Viewer

Length: 822 bp
ATGCCGCAGGCATTGCGATTTACTGGGGTCAAAATGGGAATGAGGGTACCTTGGAAGAGACCTGTGCCACAGGCAACTATGACTGTGAACATAGCTTTTCTCCCAACATTCGGCAATGACCAGACTCCCATGATAAACCTAGCCGGACATTGTGATCCTTCCACTAATGGCTGCACCGGTTTGAGCTCCGACATTAAGTCGTGCCAAGCAAAAGGGGTCAAGGTGATTCTTTCGATCGGAGGAGGAGCAGGGAGCTACTACCTCACATCTAAAGAGGATGCCAGGCAGGTTGCAATATATTTGTGGAACAATTTCTTGGGAGGAACTTCATCTTCTCGCCCTCTTGGTGACGCTGTTTTGGATGGAATTGATTTTGATATCGAGGGAGGAACAAACCTACATTGGGATGACCTCGCTACGTTCCTCTCTGCATACAGCAACCAAGGTAAGAAGGTTTACTTGACCGCAGCGCCGCAGTGCCCTTTCCCTGATGCTTGGGTTGGAGGTGCCCTCAAGACGGGCCTTTTTGACTACGTTTGGGTCCAATTCTACAACAACCCTCCATGCCAATACTCGGCCGATATCAGCAACCTTGTAAGTGCATGGAATCAGTGGGTTTCCGATATTCCGGCCACCAAGATATTCCTTGGTCTACCAGCTGCTCCTGAAGCTGCTGGAAGTGGCTTTATTCCTGTCAATGATCTCACTTCTAAAGTGCTTCCAGCAATTAAAGGTTCGTCCAAGTATGGAGGGGTCATGCTTTGGTCCAAGTATTATGATGATCAGACTAAATACAGTGCTTCCATCAAGAACTACCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

273

Amino Acids

29.49

Weight (kDa)

7.56

Isoelectric Point (pI)

37.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 24 - 221 1.2e-17 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000601)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24090
fragaria_vesca FvH4_7g15560 FvH4_7g30230 FvH4_7g30780
malus_domestica MD01G1071700.v1.1 MD07G1282300.v1.1 MD07G1282500.v1.1 MD07G1282800.v1.1
prunus_persica Prupe.2G177900_v2.0.a1 Prupe.2G179300_v2.0.a1 Prupe.2G304600_v2.0.a1 Prupe.2G305100_v2.0.a1
pyrus_communis pycom01g22220 pycom07g25620 pycom07g25710
rosa_chinensis RchiOBHm_Chr1g0358211 RchiOBHm_Chr1g0358221 RchiOBHm_Chr1g0358231 RchiOBHm_Chr1g0378291 RchiOBHm_Chr1g0379611 RchiOBHm_Chr1g0379721 RchiOBHm_Chr2g0139211 RchiOBHm_Chr2g0156271 RchiOBHm_Chr3g0487471 RchiOBHm_Chr4g0400341 RchiOBHm_Chr5g0069621
rosa_laevigata RLG00000009194 RLG00000019795 RLG00000020900 RLG00000020903 RLG00000026363 RLG00000026376 RLG00000026390 RLG00000027981 RLG00000027982
rosa_multiflora Rmu_co8335173.1_g000001 Rmu_co8492325.1_g000001 Rmu_sc0001803.1_g000006 Rmu_sc0002071.1_g000010 Rmu_sc0002831.1_g000023 Rmu_sc0007072.1_g000002 Rmu_sc0013028.1_g000008 Rmu_sc0013545.1_g000007 Rmu_sc0034114.1_g000001
rosa_roxburghii Rroxscaffold_2G00105550 Rroxscaffold_4G00279940 Rroxscaffold_4G00280040 Rroxscaffold_4G00298330 Rroxscaffold_4G00298350
rosa_rugosa Rorug01G0259900 Rorug01G0407900 Rorug01G0414900 Rorug02G0355600 Rorug02G0452700
rosa_samantha Rh1AG273700 Rh1AG273800 Rh1AG429400 Rh1AG438400 Rh1BG240900 Rh1BG394900 Rh1CG257400 Rh1CG407900 Rh2BG416300 Rh2BG530900 Rh2CG391800 Rh2CG504200 Rh2DG425500 Rh2DG539900 Rh4AG100500 Rh6BG404000 Rh7CG152600
rosa_wichuraiana Rw0G019260 Rw1G024460 Rw1G024470 Rw1G037590 Rw1G038110 Rw1G038190 Rw2G033140 Rw2G042780 Rw5G042510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 277
Acc65I GGTACC 1 cut(s) 46
AccB1I GGYRCC 2 cut(s) 46, 506
AccI GTMKAC 1 cut(s) 652
AciI CCGC 3 cut(s) 5, 465, 473
AclWI GGATC 1 cut(s) 149
AcoI YGGCCR 2 cut(s) 576, 630
AcuI CTGAAG 1 cut(s) 687
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 4 cut(s) 110, 403, 517, 574
AgeI ACCGGT 1 cut(s) 176
AhdI GACNNNNNGTC 1 cut(s) 196
AjnI CCWGG 1 cut(s) 281
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AluBI AGCT 5 cut(s) 95, 186, 255, 659, 671
AluI AGCT 5 cut(s) 95, 186, 255, 659, 671
Alw21I GWGCWC 1 cut(s) 188
Alw26I GTCTC 1 cut(s) 52
AlwI GGATC 1 cut(s) 149
AoxI GGCC 3 cut(s) 520, 576, 630
ApeKI GCWGC 4 cut(s) 171, 467, 659, 671
AsiGI ACCGGT 1 cut(s) 176
Asp700I GAANNNNTTC 1 cut(s) 311
Asp718I GGTACC 1 cut(s) 46
AspLEI GCGC 1 cut(s) 472
AspS9I GGNCC 3 cut(s) 520, 541, 765
AsuHPI GGTGA 2 cut(s) 235, 359
AvaII GGWCC 2 cut(s) 541, 765
BaeGI GKGCMC 2 cut(s) 482, 511
BanI GGYRCC 2 cut(s) 46, 506
BanII GRGCYC 1 cut(s) 188
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 4 cut(s) 158, 479, 646, 658
BccI CCATC 2 cut(s) 356, 812
BciT130I CCWGG 1 cut(s) 283
BclI TGATCA 1 cut(s) 781
BcoDI GTCTC 1 cut(s) 52
BfaI CTAG 2 cut(s) 140, 820
BfoI RGCGCY 1 cut(s) 473
BfuAI ACCTGC 1 cut(s) 277
BisI GCNGC 6 cut(s) 5, 172, 468, 473, 660, 672
BlsI GCNGC 6 cut(s) 6, 173, 469, 474, 661, 673
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 2 cut(s) 541, 765
BmeRI GACNNNNNGTC 1 cut(s) 196
BmgT120I GGNCC 3 cut(s) 520, 541, 765
BmiI GGNNCC 3 cut(s) 48, 508, 542
BmrFI CCNGG 1 cut(s) 283
BmrI ACTGGG 1 cut(s) 33
BmsI GCATC 2 cut(s) 268, 481
BmuI ACTGGG 1 cut(s) 33
BplI GAGNNNNNCTC 2 cut(s) 495, 527
BpuEI CTTGAG 1 cut(s) 497
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 3 cut(s) 50, 442, 646
BsaWI WCCGGW 1 cut(s) 176
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 403, 517, 574
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 1 cut(s) 28
Bse3DI GCAATG 2 cut(s) 11, 121
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 3 cut(s) 50, 442, 646
BseGI GGATG 3 cut(s) 283, 367, 412
BseLI CCNNNNNNNGG 4 cut(s) 110, 403, 517, 574
BseMI GCAATG 2 cut(s) 11, 121
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 2 cut(s) 255, 258
BseSI GKGCMC 2 cut(s) 482, 511
BseX3I CGGCCG 1 cut(s) 576
BseXI GCAGC 4 cut(s) 158, 479, 646, 658
BsgI GTGCAG 1 cut(s) 157
Bsh1285I CGRYCG 2 cut(s) 237, 579
BshFI GGCC 3 cut(s) 522, 578, 632
BshNI GGYRCC 2 cut(s) 46, 506
BshTI ACCGGT 1 cut(s) 176
BsiEI CGRYCG 2 cut(s) 237, 579
BsiHKAI GWGCWC 1 cut(s) 188
BsiSI CCGG 3 cut(s) 144, 177, 629
BslI CCNNNNNNNGG 4 cut(s) 110, 403, 517, 574
BsmAI GTCTC 1 cut(s) 52
BsnI GGCC 3 cut(s) 522, 578, 632
Bso31I GGTCTC 1 cut(s) 52
Bsp1286I GDGCHC 3 cut(s) 188, 482, 511
Bsp143I GATC 4 cut(s) 154, 234, 700, 781
BspACI CCGC 3 cut(s) 5, 465, 473
BspANI GGCC 3 cut(s) 522, 578, 632
BspLI GGNNCC 3 cut(s) 48, 508, 542
BspMI ACCTGC 1 cut(s) 277
BspPI GGATC 1 cut(s) 149
BspT107I GGYRCC 2 cut(s) 46, 506
BspTNI GGTCTC 1 cut(s) 52
BsrDI GCAATG 2 cut(s) 11, 121
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 1 cut(s) 28
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 3 cut(s) 50, 442, 646
BssMI GATC 4 cut(s) 154, 234, 700, 781
BssT1I CCWWGG 3 cut(s) 50, 442, 646
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 2 cut(s) 85, 797
Bst6I CTCTTC 1 cut(s) 50
BstAPI GCANNNNNTGC 1 cut(s) 13
BstC8I GCNNGC 1 cut(s) 9
BstF5I GGATG 3 cut(s) 283, 367, 412
BstH2I RGCGCY 1 cut(s) 473
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 4 cut(s) 157, 237, 703, 784
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 4 cut(s) 154, 234, 700, 781
BstMCI CGRYCG 2 cut(s) 237, 579
BstMWI GCNNNNNNNGC 2 cut(s) 13, 668
BstNI CCWGG 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 281
BstSLI GKGCMC 2 cut(s) 482, 511
BstV1I GCAGC 4 cut(s) 158, 479, 646, 658
BstZI CGGCCG 1 cut(s) 576
BsuRI GGCC 3 cut(s) 522, 578, 632
BtsCI GGATG 3 cut(s) 283, 367, 412
BtsI GCAGTG 1 cut(s) 482
BtsIMutI CAGTG 3 cut(s) 482, 617, 802
BveI ACCTGC 1 cut(s) 277
Cac8I GCNNGC 1 cut(s) 9
CfoI GCGC 1 cut(s) 472
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 3 cut(s) 520, 541, 765
CseI GACGC 1 cut(s) 359
Csp6I GTAC 1 cut(s) 47
CspAI ACCGGT 1 cut(s) 176
CviAII CATG 4 cut(s) 130, 564, 603, 757
CviQI GTAC 1 cut(s) 47
DpnI GATC 4 cut(s) 156, 236, 702, 783
DpnII GATC 4 cut(s) 154, 234, 700, 781
DriI GACNNNNNGTC 1 cut(s) 196
EaeI YGGCCR 2 cut(s) 576, 630
EagI CGGCCG 1 cut(s) 576
Eam1104I CTCTTC 1 cut(s) 50
Eam1105I GACNNNNNGTC 1 cut(s) 196
EarI CTCTTC 1 cut(s) 50
Ecl136II GAGCTC 1 cut(s) 186
EclXI CGGCCG 1 cut(s) 576
Eco130I CCWWGG 3 cut(s) 50, 442, 646
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 1 cut(s) 52
Eco32I GATATC 2 cut(s) 379, 583
Eco47I GGWCC 2 cut(s) 541, 765
Eco52I CGGCCG 1 cut(s) 576
Eco53kI GAGCTC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 687
EcoICRI GAGCTC 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 281
EcoRV GATATC 2 cut(s) 379, 583
EcoT14I CCWWGG 3 cut(s) 50, 442, 646
EcoT38I GRGCYC 1 cut(s) 188
ErhI CCWWGG 3 cut(s) 50, 442, 646
FaeI CATG 4 cut(s) 133, 567, 606, 760
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FatI CATG 4 cut(s) 129, 563, 602, 756
FbaI TGATCA 1 cut(s) 781
FblI GTMKAC 1 cut(s) 652
Fnu4HI GCNGC 6 cut(s) 5, 172, 468, 473, 660, 672
FokI GGATG 3 cut(s) 290, 374, 419
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 6 cut(s) 5, 172, 468, 473, 660, 672
FspBI CTAG 2 cut(s) 140, 820
GlaI GCGC 1 cut(s) 471
GluI GCNGC 6 cut(s) 5, 172, 468, 473, 660, 672
HaeII RGCGCY 1 cut(s) 473
HaeIII GGCC 3 cut(s) 522, 578, 632
HapII CCGG 3 cut(s) 144, 177, 629
HgaI GACGC 1 cut(s) 359
HhaI GCGC 1 cut(s) 472
Hin1II CATG 4 cut(s) 133, 567, 606, 760
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HinfI GANTC 3 cut(s) 124, 226, 607
HpaII CCGG 3 cut(s) 144, 177, 629
HphI GGTGA 2 cut(s) 235, 359
Hpy166II GTNNAC 3 cut(s) 88, 457, 653
Hpy188I TCNGA 4 cut(s) 190, 239, 622, 786
Hpy188III TCNNGA 3 cut(s) 514, 665, 808
Hpy8I GTNNAC 3 cut(s) 88, 457, 653
HpyAV CCTTC 2 cut(s) 168, 445
HpyCH4III ACNGT 2 cut(s) 85, 797
HpyCH4IV ACGT 2 cut(s) 419, 534
HpyCH4V TGCA 4 cut(s) 174, 293, 431, 602
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 668
HpySE526I ACGT 2 cut(s) 419, 534
Hsp92II CATG 4 cut(s) 133, 567, 606, 760
HspAI GCGC 1 cut(s) 470
KpnI GGTACC 1 cut(s) 50
Ksp22I TGATCA 1 cut(s) 781
Kzo9I GATC 4 cut(s) 154, 234, 700, 781
LmnI GCTCC 4 cut(s) 191, 245, 252, 667
Lsp1109I GCAGC 4 cut(s) 158, 479, 646, 658
LweI GCATC 2 cut(s) 268, 481
MaeI CTAG 2 cut(s) 140, 820
MaeII ACGT 2 cut(s) 419, 534
MaeIII GTNAC 1 cut(s) 347
MalI GATC 4 cut(s) 156, 236, 702, 783
MboI GATC 4 cut(s) 154, 234, 700, 781
MboII GAAGA 2 cut(s) 67, 324
MhlI GDGCHC 3 cut(s) 188, 482, 511
MluCI AATT 4 cut(s) 310, 366, 545, 726
MlyI GAGTC 1 cut(s) 118
MmeI TCCRAC 2 cut(s) 213, 481
MroXI GAANNNNTTC 1 cut(s) 311
MseI TTAA 2 cut(s) 195, 729
MslI CAYNNNNRTG 1 cut(s) 405
MspA1I CMGCKG 1 cut(s) 659
MspI CCGG 3 cut(s) 144, 177, 629
MspR9I CCNGG 1 cut(s) 283
MvaI CCWGG 1 cut(s) 283
MwoI GCNNNNNNNGC 2 cut(s) 13, 668
NdeII GATC 4 cut(s) 154, 234, 700, 781
NlaIII CATG 4 cut(s) 133, 567, 606, 760
NlaIV GGNNCC 3 cut(s) 48, 508, 542
NmeAIII GCCGAG 1 cut(s) 554
NmuCI GTSAC 1 cut(s) 347
PdmI GAANNNNTTC 1 cut(s) 311
PfeI GAWTC 2 cut(s) 226, 607
PinAI ACCGGT 1 cut(s) 176
PkrI GCNGC 6 cut(s) 6, 173, 469, 474, 661, 673
Ple19I CGATCG 1 cut(s) 237
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
Psp124BI GAGCTC 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 3 cut(s) 48, 508, 542
PspPI GGNCC 3 cut(s) 520, 541, 765
PvuI CGATCG 1 cut(s) 237
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 405
SacI GAGCTC 1 cut(s) 188
SaqAI TTAA 2 cut(s) 195, 729
SatI GCNGC 6 cut(s) 5, 172, 468, 473, 660, 672
Sau3AI GATC 4 cut(s) 154, 234, 700, 781
Sau96I GGNCC 3 cut(s) 520, 541, 765
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 1 cut(s) 283
SduI GDGCHC 3 cut(s) 188, 482, 511
SfaNI GCATC 2 cut(s) 268, 481
SinI GGWCC 2 cut(s) 541, 765
SmiMI CAYNNNNRTG 1 cut(s) 405
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 4 cut(s) 310, 366, 545, 726
SsiI CCGC 3 cut(s) 5, 465, 473
SspMI CTAG 2 cut(s) 140, 820
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 1 cut(s) 281
StyI CCWWGG 3 cut(s) 50, 442, 646
TaaI ACNGT 2 cut(s) 85, 797
TaiI ACGT 2 cut(s) 422, 537
TaqI TCGA 2 cut(s) 233, 381
TasI AATT 4 cut(s) 310, 366, 545, 726
TauI GCSGC 2 cut(s) 7, 475
TfiI GAWTC 2 cut(s) 226, 607
Tru1I TTAA 2 cut(s) 195, 729
Tru9I TTAA 2 cut(s) 195, 729
TscAI CASTG 3 cut(s) 482, 617, 802
TseFI GTSAC 1 cut(s) 347
TseI GCWGC 4 cut(s) 171, 467, 659, 671
Tsp45I GTSAC 1 cut(s) 347
TspDTI ATGAA 1 cut(s) 318
TspRI CASTG 3 cut(s) 482, 617, 802
VpaK11BI GGWCC 2 cut(s) 541, 765
XmiI GTMKAC 1 cut(s) 652
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 2 cut(s) 140, 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.