AT1G12550

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000446
Physical Location & Seq
Forward (+)
4274531 .. 4276031
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G12550.1

Sequence Viewer

Length: 972 bp
ATGGCGGAATCTTCAGAGCCTCCGGTTGTTCTCCTTCACCGACCACCGTCTCTAACTTTCATGGACGAGATTCTGACTCGTGAATTCCGTACTCTCATCACCGACACTTCCTCATCGGAATCACTCCCGAGCTTCTTCCCCCGTCACGCCTCCTCCGCCAGAGCCTTCGTTATCAGCGGCAGACTTCCGGTAACCGATGAACTCCTTTCTCATCTCCCTTCTCTCCAGATTCTCGTCTGCACTAGCGTCGGCATCGATCACATCGATCTCGCCGCATGCAAGCGTCGCGGCATCGTTATCACCAACGCTGGCAACGCGTTTTCCGATGACGTTGCTGATTGTGCCGTCGGTTTGCTTATTAGCGTTCTCCGTCGTATTCCTGCCGCCGATCGTTACGTCCGGTCTGGTAATTGGGCGAAATTTGGGGATTTTCAATTAGGGTCCAAGGTAAGTGGGAAGAGAGTTGGGATAGTTGGATTGGGGAGCATTGGATCATTTGTTGCTAAAAGACTTGAATCATTTGGCTGTGTTATCTCTTACAACTCAAGGAGTCAGAAACAGAGTAGTCCATACCGGTATTACTCTGACATTCTCTCGTTAGCAGAGAACAACGATGTACTTGTCCTCTGCTGCTCTTTGACAGACGAAACGCACCATATTGTGAATAGAGAAGTGATGGAGTTGCTTGGTAAGGATGGGGTTGTGATCAATGTGGGACGAGGAAAGTTGATTGATGAGAAGGAGATGGTCAAGTGTTTGGTTGACGGTGTGATTGGTGGTGCTGGTTTAGATGTGTTTGAGAATGAACCGGCAGTTCCTCAGGAGTTGTTTGGTTTGGATAATGTAGTGTTGTCTCCTCATTTTGCTGTGGCTACACCAGGGTCTTTGGACAATGTTGCACAGATTGCTTTAGCTAACTTGAAGGCGTTTTTCTCGAACCGGCCTTTGCTTTCTCCGGTTCAATTGGATTGA

Protein Analysis

323

Amino Acids

34.91

Weight (kDa)

5.68

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 33 - 320 1.3e-24 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 116 - 289 2.3e-50 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 288, 317
AciI CCGC 6 cut(s) 5, 156, 177, 273, 288, 384
AclWI GGATC 1 cut(s) 499
AcsI RAATTY 2 cut(s) 83, 419
AfaI GTAC 2 cut(s) 91, 618
AflIII ACRYGT 1 cut(s) 315
AgeI ACCGGT 1 cut(s) 573
AgsI TTSAA 4 cut(s) 434, 515, 922, 962
AjnI CCWGG 1 cut(s) 877
AluBI AGCT 2 cut(s) 132, 914
AluI AGCT 2 cut(s) 132, 914
Alw26I GTCTC 2 cut(s) 54, 858
AlwI GGATC 1 cut(s) 499
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 1 cut(s) 941
ApeKI GCWGC 1 cut(s) 630
ApoI RAATTY 2 cut(s) 83, 419
AsiGI ACCGGT 1 cut(s) 573
AspS9I GGNCC 1 cut(s) 441
AsuHPI GGTGA 3 cut(s) 29, 91, 292
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 1 cut(s) 441
AxyI CCTNAGG 1 cut(s) 819
BauI CACGAG 1 cut(s) 78
BbvI GCAGC 1 cut(s) 617
BccI CCATC 3 cut(s) 670, 689, 739
BceAI ACGGC 1 cut(s) 329
BcgI CGANNNNNNTGC 4 cut(s) 229, 263, 314, 348
BciT130I CCWGG 1 cut(s) 879
BclI TGATCA 1 cut(s) 705
BcoDI GTCTC 2 cut(s) 54, 858
BfaI CTAG 1 cut(s) 243
BisI GCNGC 5 cut(s) 178, 273, 289, 384, 631
BlsI GCNGC 5 cut(s) 179, 274, 290, 385, 632
Bme1390I CCNGG 1 cut(s) 879
Bme18I GGWCC 1 cut(s) 441
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 441
BmiI GGNNCC 1 cut(s) 442
BmrFI CCNGG 1 cut(s) 879
BmsI GCATC 2 cut(s) 261, 300
BoxI GACNNNNGTC 1 cut(s) 46
BpmI CTGGAG 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 529
Bsa29I ATCGAT 2 cut(s) 255, 264
BsaJI CCNNGG 2 cut(s) 444, 878
BsaWI WCCGGW 5 cut(s) 22, 187, 399, 573, 955
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bse118I RCCGGY 3 cut(s) 573, 808, 939
Bse21I CCTNAGG 1 cut(s) 819
BseBI CCWGG 1 cut(s) 879
BseCI ATCGAT 2 cut(s) 255, 264
BseDI CCNNGG 2 cut(s) 444, 878
BseGI GGATG 1 cut(s) 700
BseMII CTCAG 1 cut(s) 833
BseRI GAGGAG 2 cut(s) 142, 846
BseXI GCAGC 1 cut(s) 617
BsgI GTGCAG 1 cut(s) 223
Bsh1236I CGCG 2 cut(s) 288, 317
Bsh1285I CGRYCG 1 cut(s) 391
BshFI GGCC 1 cut(s) 943
BshTI ACCGGT 1 cut(s) 573
BshVI ATCGAT 2 cut(s) 255, 264
BsiEI CGRYCG 1 cut(s) 391
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 7 cut(s) 23, 188, 400, 574, 809, 940, 956
BslFI GGGAC 1 cut(s) 729
BsmAI GTCTC 2 cut(s) 54, 858
BsmBI CGTCTC 1 cut(s) 54
BsmFI GGGAC 1 cut(s) 729
BsnI GGCC 1 cut(s) 943
BsoBI CYCGRG 1 cut(s) 127
Bsp143I GATC 5 cut(s) 256, 265, 388, 491, 705
BspACI CCGC 6 cut(s) 5, 156, 177, 273, 288, 384
BspANI GGCC 1 cut(s) 943
BspCNI CTCAG 1 cut(s) 832
BspDI ATCGAT 2 cut(s) 255, 264
BspFNI CGCG 2 cut(s) 288, 317
BspLI GGNNCC 1 cut(s) 442
BspPI GGATC 1 cut(s) 499
BsrFI RCCGGY 3 cut(s) 573, 808, 939
BssAI RCCGGY 3 cut(s) 573, 808, 939
BssECI CCNNGG 2 cut(s) 444, 878
BssMI GATC 5 cut(s) 256, 265, 388, 491, 705
BssSI CACGAG 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 444
Bst2BI CACGAG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 879
Bst4CI ACNGT 2 cut(s) 48, 767
Bst6I CTCTTC 1 cut(s) 452
BstAPI GCANNNNNTGC 1 cut(s) 905
BstC8I GCNNGC 3 cut(s) 277, 281, 310
BstDEI CTNAG 1 cut(s) 819
BstEII GGTNACC 1 cut(s) 190
BstF5I GGATG 1 cut(s) 700
BstFNI CGCG 2 cut(s) 288, 317
BstKTI GATC 5 cut(s) 259, 268, 391, 494, 708
BstMAI GTCTC 2 cut(s) 54, 858
BstMBI GATC 5 cut(s) 256, 265, 388, 491, 705
BstMCI CGRYCG 1 cut(s) 391
BstMWI GCNNNNNNNGC 5 cut(s) 155, 285, 314, 341, 905
BstNI CCWGG 1 cut(s) 879
BstNSI RCATGY 1 cut(s) 279
BstPAI GACNNNNGTC 1 cut(s) 46
BstPI GGTNACC 1 cut(s) 190
BstSCI CCNGG 1 cut(s) 877
BstUI CGCG 2 cut(s) 288, 317
BstV1I GCAGC 1 cut(s) 617
Bsu15I ATCGAT 2 cut(s) 255, 264
Bsu36I CCTNAGG 1 cut(s) 819
BsuRI GGCC 1 cut(s) 943
BsuTUI ATCGAT 2 cut(s) 255, 264
BtsCI GGATG 1 cut(s) 700
Cac8I GCNNGC 3 cut(s) 277, 281, 310
Cfr10I RCCGGY 3 cut(s) 573, 808, 939
Cfr13I GGNCC 1 cut(s) 441
ClaI ATCGAT 2 cut(s) 255, 264
CseI GACGC 2 cut(s) 235, 272
Csp6I GTAC 2 cut(s) 90, 617
CspAI ACCGGT 1 cut(s) 573
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 2 cut(s) 61, 276
CviJI RGCY 7 cut(s) 19, 132, 164, 525, 872, 914, 943
CviKI_1 RGCY 7 cut(s) 19, 132, 164, 525, 872, 914, 943
CviQI GTAC 2 cut(s) 90, 617
DdeI CTNAG 1 cut(s) 819
DpnI GATC 5 cut(s) 258, 267, 390, 493, 707
DpnII GATC 5 cut(s) 256, 265, 388, 491, 705
Eam1104I CTCTTC 1 cut(s) 452
EarI CTCTTC 1 cut(s) 452
EciI GGCGGA 2 cut(s) 20, 145
Eco130I CCWWGG 1 cut(s) 444
Eco47I GGWCC 1 cut(s) 441
Eco81I CCTNAGG 1 cut(s) 819
Eco88I CYCGRG 1 cut(s) 127
Eco91I GGTNACC 1 cut(s) 190
EcoO65I GGTNACC 1 cut(s) 190
EcoRI GAATTC 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 877
EcoT14I CCWWGG 1 cut(s) 444
ErhI CCWWGG 1 cut(s) 444
Esp3I CGTCTC 1 cut(s) 54
FaeI CATG 2 cut(s) 64, 279
FaiI YATR 4 cut(s) 62, 277, 571, 657
FaqI GGGAC 1 cut(s) 729
FatI CATG 2 cut(s) 60, 275
FbaI TGATCA 1 cut(s) 705
Fnu4HI GCNGC 5 cut(s) 178, 273, 289, 384, 631
FokI GGATG 1 cut(s) 707
Fsp4HI GCNGC 5 cut(s) 178, 273, 289, 384, 631
FspBI CTAG 1 cut(s) 243
GluI GCNGC 5 cut(s) 178, 273, 289, 384, 631
GsuI CTGGAG 1 cut(s) 209
HaeIII GGCC 1 cut(s) 943
HapII CCGG 7 cut(s) 23, 188, 400, 574, 809, 940, 956
HgaI GACGC 2 cut(s) 235, 272
Hin1II CATG 2 cut(s) 64, 279
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HinfI GANTC 7 cut(s) 8, 70, 76, 119, 229, 515, 550
HpaII CCGG 7 cut(s) 23, 188, 400, 574, 809, 940, 956
HphI GGTGA 3 cut(s) 29, 91, 292
Hpy166II GTNNAC 1 cut(s) 763
Hpy188I TCNGA 6 cut(s) 16, 75, 118, 325, 555, 586
Hpy188III TCNNGA 5 cut(s) 80, 127, 226, 821, 934
Hpy8I GTNNAC 1 cut(s) 763
Hpy99I CGWCG 4 cut(s) 251, 288, 350, 375
HpyAV CCTTC 5 cut(s) 44, 175, 228, 733, 916
HpyCH4III ACNGT 2 cut(s) 48, 767
HpyCH4IV ACGT 2 cut(s) 330, 396
HpyCH4V TGCA 3 cut(s) 240, 279, 899
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 285, 314, 341, 905
HpyF3I CTNAG 1 cut(s) 819
HpySE526I ACGT 2 cut(s) 330, 396
Hsp92II CATG 2 cut(s) 64, 279
Ksp22I TGATCA 1 cut(s) 705
Kzo9I GATC 5 cut(s) 256, 265, 388, 491, 705
LmnI GCTCC 1 cut(s) 483
Lsp1109I GCAGC 1 cut(s) 617
LweI GCATC 2 cut(s) 261, 300
MaeI CTAG 1 cut(s) 243
MaeII ACGT 2 cut(s) 330, 396
MaeIII GTNAC 3 cut(s) 143, 190, 392
MalI GATC 5 cut(s) 258, 267, 390, 493, 707
MboI GATC 5 cut(s) 256, 265, 388, 491, 705
MboII GAAGA 3 cut(s) 3, 127, 469
MfeI CAATTG 1 cut(s) 962
MluCI AATT 5 cut(s) 83, 409, 419, 434, 962
MluI ACGCGT 1 cut(s) 315
MlyI GAGTC 2 cut(s) 70, 559
MmeI TCCRAC 1 cut(s) 454
MnlI CCTC 8 cut(s) 30, 121, 160, 163, 635, 713, 828, 867
MspA1I CMGCKG 1 cut(s) 177
MspI CCGG 7 cut(s) 23, 188, 400, 574, 809, 940, 956
MspR9I CCNGG 1 cut(s) 879
MunI CAATTG 1 cut(s) 962
MvaI CCWGG 1 cut(s) 879
MvnI CGCG 2 cut(s) 288, 317
MwoI GCNNNNNNNGC 5 cut(s) 155, 285, 314, 341, 905
NdeII GATC 5 cut(s) 256, 265, 388, 491, 705
NlaIII CATG 2 cut(s) 64, 279
NlaIV GGNNCC 1 cut(s) 442
NmuCI GTSAC 1 cut(s) 143
NspI RCATGY 1 cut(s) 279
PaeI GCATGC 1 cut(s) 279
PcsI WCGNNNNNNNCGW 3 cut(s) 85, 261, 321
PfeI GAWTC 5 cut(s) 8, 70, 119, 229, 515
PinAI ACCGGT 1 cut(s) 573
PkrI GCNGC 5 cut(s) 179, 274, 290, 385, 632
Ple19I CGATCG 1 cut(s) 391
PleI GAGTC 2 cut(s) 70, 558
PpsI GAGTC 2 cut(s) 70, 558
PshAI GACNNNNGTC 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 877
PspEI GGTNACC 1 cut(s) 190
PspGI CCWGG 1 cut(s) 877
PspN4I GGNNCC 1 cut(s) 442
PspPI GGNCC 1 cut(s) 441
PvuI CGATCG 1 cut(s) 391
RsaI GTAC 2 cut(s) 91, 618
RsaNI GTAC 2 cut(s) 90, 617
SatI GCNGC 5 cut(s) 178, 273, 289, 384, 631
Sau3AI GATC 5 cut(s) 256, 265, 388, 491, 705
Sau96I GGNCC 1 cut(s) 441
SchI GAGTC 2 cut(s) 70, 559
ScrFI CCNGG 1 cut(s) 879
SetI ASST 5 cut(s) 134, 333, 399, 450, 916
SfaNI GCATC 2 cut(s) 261, 300
SinI GGWCC 1 cut(s) 441
SmlI CTYRAG 1 cut(s) 544
SmoI CTYRAG 1 cut(s) 544
SphI GCATGC 1 cut(s) 279
Sse9I AATT 5 cut(s) 83, 409, 419, 434, 962
SsiI CCGC 6 cut(s) 5, 156, 177, 273, 288, 384
SspMI CTAG 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 877
StyI CCWWGG 1 cut(s) 444
TaaI ACNGT 2 cut(s) 48, 767
TaiI ACGT 2 cut(s) 333, 399
TaqI TCGA 3 cut(s) 255, 264, 935
TasI AATT 5 cut(s) 83, 409, 419, 434, 962
TatI WGTACW 1 cut(s) 616
TauI GCSGC 4 cut(s) 180, 275, 291, 386
TfiI GAWTC 5 cut(s) 8, 70, 119, 229, 515
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 1 cut(s) 630
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 3 cut(s) 49, 213, 819
TspGWI ACGGA 2 cut(s) 77, 359
VpaK11BI GGWCC 1 cut(s) 441
XapI RAATTY 2 cut(s) 83, 419
XceI RCATGY 1 cut(s) 279
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.