MD07G1124400.v1.1

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000446
Physical Location & Seq
Reverse (-)
17392208 .. 17394345
2138 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1124400.v1.1.491

Sequence Viewer

Length: 981 bp
ATGGCACAAGATAATCCCACCCAACCCCATCAAGTCCTGCTTCTTCTCCCAAGCCACTGCTTCACTGCCCTGGAGTCCAAAGCCTCTCACAAGTTCCACTTCCTCAAAGCCTGGGACTCCCCTCTCCCTCAGGCCCAGTTCCTCCAGTCCCACGCTACCTCCTCCCAGGTCCTCCTCTCTTTCCCCAAAGAAACCCCCATCACCGCCCAGCTTCTCCAATTACTCCCCTCCTTGAAGCTCCTAATCACCATCAGCGCAGGCCTCGACCGCGTTGATTTGGCTGCGTGTCGAGCCCGCGGCGTGCGCATTGCCAATTCCTCTCCAATCTTCGCTGGCGATGTCGCTGACATGGCTGTGGGGTTGTTTCTGGACGTCATGAGGAAGATTTCCGCTGCGAATCGGTATGTCCGAGATGGGTTTTGGGTTAGCGGAGGAGATTTTGTTCTCGGTTCTAAGGTAGGAGGGAAGCGAGTTGGTATAGTTGGATTGGGACATATTGGCCTACAAGTTGCTAAAAGACTTGAGGCATTTGACTGCAAAATCTTGTACAACTCAAGGAAGGAAAAACCCTCTGTTTCTTACCCTTTCTATTCCGATGTTTGTGAACTTGCGGCTAACAGCGATGTTCTTGTCATTTGCTGCGCGTTGACTGCTCAAACCCGCCACATGATTAACAAGAAAGTCTTGTTGGCGTTGGGAAGAGAGGGAGTGATTGTTAATGTAGGGCGTGGAGCTATTATCGATGAGAAGGAAATGGTCCAGTGTTTGGTGCAAGGAGAGATCAAAGGTGCAGGCTTGGATGTGTTTGAGAATGAGCCTCAAGTTCCGAAAGAGCTCTTTGAACTAGATAATGTTGTTTTGTCGCCGCATAAAGCTGTCTTGACAGCAGACTGTTTCACGGATGTGACTGATCTAGCAATGGCGAATTTGGAGGCATTCTTCTCAAATAAACCATTGCTTTCCGAAGTGGTGAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

327

Amino Acids

35.5

Weight (kDa)

6.71

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 54 - 323 1.9e-15 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 120 - 292 1.6e-51 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 375
Acc16I TGCGCA 1 cut(s) 305
AccB7I CCANNNNNTGG 1 cut(s) 766
AccII CGCG 3 cut(s) 270, 297, 644
AciI CCGC 9 cut(s) 204, 268, 295, 297, 390, 429, 611, 661, 866
AcsI RAATTY 1 cut(s) 925
AcyI GRCGYC 1 cut(s) 372
AfaI GTAC 1 cut(s) 548
AfiI CCNNNNNNNGG 1 cut(s) 766
AgsI TTSAA 2 cut(s) 235, 842
AjnI CCWGG 3 cut(s) 69, 110, 165
AjuI GAANNNNNNNTTGG 2 cut(s) 671, 703
AleI CACNNNNGTG 1 cut(s) 902
AloI GAACNNNNNNTCC 2 cut(s) 426, 458
AluBI AGCT 5 cut(s) 211, 238, 734, 835, 875
AluI AGCT 5 cut(s) 211, 238, 734, 835, 875
Alw21I GWGCWC 1 cut(s) 837
AoxI GGCC 3 cut(s) 132, 259, 499
ApeKI GCWGC 3 cut(s) 281, 392, 639
ApoI RAATTY 1 cut(s) 925
AspLEI GCGC 3 cut(s) 257, 306, 644
AspS9I GGNCC 3 cut(s) 133, 169, 757
AsuHPI GGTGA 2 cut(s) 193, 238
AvaII GGWCC 2 cut(s) 169, 757
AxyI CCTNAGG 1 cut(s) 129
BanII GRGCYC 2 cut(s) 295, 837
Bbv12I GWGCWC 1 cut(s) 837
BbvI GCAGC 3 cut(s) 268, 379, 626
BccI CCATC 4 cut(s) 36, 206, 257, 407
BciT130I CCWGG 3 cut(s) 71, 112, 167
BfaI CTAG 2 cut(s) 845, 914
BisI GCNGC 6 cut(s) 282, 298, 393, 612, 640, 866
BlsI GCNGC 6 cut(s) 283, 299, 394, 613, 641, 867
Bme1390I CCNGG 3 cut(s) 71, 112, 167
Bme18I GGWCC 2 cut(s) 169, 757
BmgT120I GGNCC 3 cut(s) 133, 169, 757
BmrFI CCNGG 3 cut(s) 71, 112, 167
BmrI ACTGGG 1 cut(s) 130
BmuI ACTGGG 1 cut(s) 130
BpmI CTGGAG 2 cut(s) 92, 128
BpuEI CTTGAG 3 cut(s) 538, 542, 804
Bsa29I ATCGAT 1 cut(s) 741
BsaHI GRCGYC 1 cut(s) 372
BsaJI CCNNGG 4 cut(s) 69, 111, 165, 295
BsaXI ACNNNNNCTCC 6 cut(s) 143, 173, 426, 456, 699, 729
Bsc4I CCNNNNNNNGG 1 cut(s) 766
Bse1I ACTGG 3 cut(s) 136, 145, 760
Bse21I CCTNAGG 1 cut(s) 129
Bse3DI GCAATG 3 cut(s) 306, 924, 953
BseBI CCWGG 3 cut(s) 71, 112, 167
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 4 cut(s) 69, 111, 165, 295
BseGI GGATG 2 cut(s) 805, 907
BseLI CCNNNNNNNGG 1 cut(s) 766
BseMI GCAATG 3 cut(s) 306, 924, 953
BseMII CTCAG 1 cut(s) 143
BseNI ACTGG 3 cut(s) 136, 145, 760
BseRI GAGGAG 3 cut(s) 151, 164, 447
BseXI GCAGC 3 cut(s) 268, 379, 626
BseYI CCCAGC 1 cut(s) 207
BsgI GTGCAG 1 cut(s) 810
Bsh1236I CGCG 3 cut(s) 270, 297, 644
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 3 cut(s) 134, 261, 501
BshVI ATCGAT 1 cut(s) 741
BsiEI CGRYCG 1 cut(s) 268
BsiHKAI GWGCWC 1 cut(s) 837
BslFI GGGAC 3 cut(s) 128, 133, 504
BslI CCNNNNNNNGG 1 cut(s) 766
BsmFI GGGAC 3 cut(s) 128, 133, 504
BsmI GAATGC 1 cut(s) 935
BsnI GGCC 3 cut(s) 134, 261, 501
Bsp1286I GDGCHC 2 cut(s) 295, 837
Bsp1407I TGTACA 1 cut(s) 546
Bsp143I GATC 2 cut(s) 780, 910
BspACI CCGC 9 cut(s) 204, 268, 295, 297, 390, 429, 611, 661, 866
BspANI GGCC 3 cut(s) 134, 261, 501
BspCNI CTCAG 1 cut(s) 142
BspDI ATCGAT 1 cut(s) 741
BspFNI CGCG 3 cut(s) 270, 297, 644
BspHI TCATGA 1 cut(s) 375
BsrDI GCAATG 3 cut(s) 306, 924, 953
BsrGI TGTACA 1 cut(s) 546
BsrI ACTGG 3 cut(s) 136, 145, 760
BssECI CCNNGG 4 cut(s) 69, 111, 165, 295
BssMI GATC 2 cut(s) 780, 910
BssNI GRCGYC 1 cut(s) 372
Bst2UI CCWGG 3 cut(s) 71, 112, 167
Bst4CI ACNGT 1 cut(s) 893
Bst6I CTCTTC 1 cut(s) 694
BstACI GRCGYC 1 cut(s) 372
BstAUI TGTACA 1 cut(s) 546
BstC8I GCNNGC 5 cut(s) 259, 295, 302, 334, 793
BstDEI CTNAG 2 cut(s) 129, 453
BstDSI CCRYGG 1 cut(s) 295
BstF5I GGATG 2 cut(s) 805, 907
BstFNI CGCG 3 cut(s) 270, 297, 644
BstHHI GCGC 3 cut(s) 257, 306, 644
BstKTI GATC 2 cut(s) 783, 913
BstMBI GATC 2 cut(s) 780, 910
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 5 cut(s) 267, 290, 303, 350, 650
BstNI CCWGG 3 cut(s) 71, 112, 167
BstSCI CCNGG 3 cut(s) 69, 110, 165
BstUI CGCG 3 cut(s) 270, 297, 644
BstV1I GCAGC 3 cut(s) 268, 379, 626
Bsu15I ATCGAT 1 cut(s) 741
Bsu36I CCTNAGG 1 cut(s) 129
BsuRI GGCC 3 cut(s) 134, 261, 501
BsuTUI ATCGAT 1 cut(s) 741
BtgI CCRYGG 1 cut(s) 295
BtgZI GCGATG 2 cut(s) 351, 636
BtsCI GGATG 2 cut(s) 805, 907
BtsI GCAGTG 2 cut(s) 55, 63
BtsIMutI CAGTG 3 cut(s) 55, 63, 767
Cac8I GCNNGC 5 cut(s) 259, 295, 302, 334, 793
CciI TCATGA 1 cut(s) 375
CfoI GCGC 3 cut(s) 257, 306, 644
Cfr13I GGNCC 3 cut(s) 133, 169, 757
Cfr42I CCGCGG 1 cut(s) 298
ClaI ATCGAT 1 cut(s) 741
Csp6I GTAC 1 cut(s) 547
CviAII CATG 3 cut(s) 349, 376, 667
CviQI GTAC 1 cut(s) 547
DdeI CTNAG 2 cut(s) 129, 453
DpnI GATC 2 cut(s) 782, 912
DpnII GATC 2 cut(s) 780, 910
Eam1104I CTCTTC 1 cut(s) 694
EarI CTCTTC 1 cut(s) 694
Ecl136II GAGCTC 1 cut(s) 835
Eco147I AGGCCT 1 cut(s) 261
Eco24I GRGCYC 2 cut(s) 295, 837
Eco47I GGWCC 2 cut(s) 169, 757
Eco53kI GAGCTC 1 cut(s) 835
Eco81I CCTNAGG 1 cut(s) 129
EcoICRI GAGCTC 1 cut(s) 835
EcoO109I RGGNCCY 1 cut(s) 169
EcoRII CCWGG 3 cut(s) 69, 110, 165
EcoT38I GRGCYC 2 cut(s) 295, 837
FaeI CATG 3 cut(s) 352, 379, 670
FaiI YATR 7 cut(s) 350, 377, 405, 479, 495, 668, 870
FalI AAGNNNNNCTT 6 cut(s) 24, 56, 83, 115, 668, 700
FaqI GGGAC 3 cut(s) 128, 133, 504
FatI CATG 3 cut(s) 348, 375, 666
FauI CCCGC 2 cut(s) 302, 668
Fnu4HI GCNGC 6 cut(s) 282, 298, 393, 612, 640, 866
FokI GGATG 2 cut(s) 812, 914
FriOI GRGCYC 2 cut(s) 295, 837
Fsp4HI GCNGC 6 cut(s) 282, 298, 393, 612, 640, 866
FspAI RTGCGCAY 1 cut(s) 305
FspBI CTAG 2 cut(s) 845, 914
FspI TGCGCA 1 cut(s) 305
GlaI GCGC 3 cut(s) 256, 305, 643
GluI GCNGC 6 cut(s) 282, 298, 393, 612, 640, 866
GsaI CCCAGC 1 cut(s) 211
GsuI CTGGAG 2 cut(s) 92, 128
HaeIII GGCC 3 cut(s) 134, 261, 501
HhaI GCGC 3 cut(s) 257, 306, 644
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 3 cut(s) 352, 379, 670
Hin6I GCGC 3 cut(s) 255, 304, 642
HinP1I GCGC 3 cut(s) 255, 304, 642
HincII GTYRAC 1 cut(s) 648
HindII GTYRAC 1 cut(s) 648
HinfI GANTC 3 cut(s) 74, 116, 397
HphI GGTGA 2 cut(s) 193, 238
Hpy166II GTNNAC 2 cut(s) 605, 648
Hpy188I TCNGA 4 cut(s) 410, 595, 828, 964
Hpy188III TCNNGA 3 cut(s) 368, 376, 880
Hpy8I GTNNAC 2 cut(s) 605, 648
HpyAV CCTTC 2 cut(s) 553, 742
HpyCH4III ACNGT 1 cut(s) 893
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 3 cut(s) 537, 772, 791
HpyF10VI GCNNNNNNNGC 5 cut(s) 267, 290, 303, 350, 650
HpyF3I CTNAG 2 cut(s) 129, 453
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 3 cut(s) 352, 379, 670
HspAI GCGC 3 cut(s) 255, 304, 642
KspI CCGCGG 1 cut(s) 298
Kzo9I GATC 2 cut(s) 780, 910
LmnI GCTCC 2 cut(s) 243, 731
Lsp1109I GCAGC 3 cut(s) 268, 379, 626
MaeI CTAG 2 cut(s) 845, 914
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 904
MalI GATC 2 cut(s) 782, 912
MboI GATC 2 cut(s) 780, 910
MboII GAAGA 5 cut(s) 35, 319, 394, 711, 931
MhlI GDGCHC 2 cut(s) 295, 837
MluCI AATT 4 cut(s) 218, 313, 925, 973
MlyI GAGTC 2 cut(s) 83, 110
MmeI TCCRAC 1 cut(s) 463
MseI TTAA 2 cut(s) 672, 717
MslI CAYNNNNRTG 2 cut(s) 353, 902
MspA1I CMGCKG 2 cut(s) 297, 392
MspR9I CCNGG 3 cut(s) 71, 112, 167
Mva1269I GAATGC 1 cut(s) 935
MvaI CCWGG 3 cut(s) 71, 112, 167
MvnI CGCG 3 cut(s) 270, 297, 644
MwoI GCNNNNNNNGC 5 cut(s) 267, 290, 303, 350, 650
NdeII GATC 2 cut(s) 780, 910
NlaIII CATG 3 cut(s) 352, 379, 670
NmuCI GTSAC 1 cut(s) 904
NsbI TGCGCA 1 cut(s) 305
OliI CACNNNNGTG 1 cut(s) 902
PagI TCATGA 1 cut(s) 375
PceI AGGCCT 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 406
PctI GAATGC 1 cut(s) 935
PfeI GAWTC 1 cut(s) 397
PflMI CCANNNNNTGG 1 cut(s) 766
PkrI GCNGC 6 cut(s) 283, 299, 394, 613, 641, 867
PleI GAGTC 2 cut(s) 82, 110
PpsI GAGTC 2 cut(s) 82, 110
PpuMI RGGWCCY 1 cut(s) 169
Psp124BI GAGCTC 1 cut(s) 837
Psp5II RGGWCCY 1 cut(s) 169
Psp6I CCWGG 3 cut(s) 69, 110, 165
PspFI CCCAGC 1 cut(s) 207
PspGI CCWGG 3 cut(s) 69, 110, 165
PspPI GGNCC 3 cut(s) 133, 169, 757
PspPPI RGGWCCY 1 cut(s) 169
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
RseI CAYNNNNRTG 2 cut(s) 353, 902
SacI GAGCTC 1 cut(s) 837
SacII CCGCGG 1 cut(s) 298
SaqAI TTAA 2 cut(s) 672, 717
SatI GCNGC 6 cut(s) 282, 298, 393, 612, 640, 866
Sau3AI GATC 2 cut(s) 780, 910
Sau96I GGNCC 3 cut(s) 133, 169, 757
SchI GAGTC 2 cut(s) 83, 110
ScrFI CCNGG 3 cut(s) 71, 112, 167
SduI GDGCHC 2 cut(s) 295, 837
Sfr303I CCGCGG 1 cut(s) 298
SgrBI CCGCGG 1 cut(s) 298
SinI GGWCC 2 cut(s) 169, 757
SmiMI CAYNNNNRTG 2 cut(s) 353, 902
SmlI CTYRAG 3 cut(s) 521, 553, 819
SmoI CTYRAG 3 cut(s) 521, 553, 819
Sse9I AATT 4 cut(s) 218, 313, 925, 973
SseBI AGGCCT 1 cut(s) 261
SsiI CCGC 9 cut(s) 204, 268, 295, 297, 390, 429, 611, 661, 866
SspMI CTAG 2 cut(s) 845, 914
SstI GAGCTC 1 cut(s) 837
StuI AGGCCT 1 cut(s) 261
StyD4I CCNGG 3 cut(s) 69, 110, 165
TaaI ACNGT 1 cut(s) 893
TaiI ACGT 1 cut(s) 375
TaqI TCGA 3 cut(s) 264, 289, 741
TasI AATT 4 cut(s) 218, 313, 925, 973
TatI WGTACW 1 cut(s) 546
TauI GCSGC 3 cut(s) 300, 614, 868
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 2 cut(s) 672, 717
Tru9I TTAA 2 cut(s) 672, 717
TscAI CASTG 3 cut(s) 62, 70, 767
TseFI GTSAC 1 cut(s) 904
TseI GCWGC 3 cut(s) 281, 392, 639
Tsp45I GTSAC 1 cut(s) 904
TspGWI ACGGA 1 cut(s) 914
TspRI CASTG 3 cut(s) 62, 70, 767
Van91I CCANNNNNTGG 1 cut(s) 766
VpaK11BI GGWCC 2 cut(s) 169, 757
XapI RAATTY 1 cut(s) 925
XspI CTAG 2 cut(s) 845, 914
ZraI GACGTC 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.