RLG00000028400

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000446
Physical Location & Seq
Reverse (-)
22263723 .. 22265665
1943 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028400

Sequence Viewer

Length: 972 bp
ATGGACCAGCAGTTGCAAAAGCTCCCAGATGTCCTGATCATCAAACCACCACCGGCCTTCATATCTTTCGAATCCGACTTCACCCAAAAGTTCAACATCCTCCGAGCATGGGAATCACCACTCCCTCTGGACCAGTTCCTCACCACTCACGCCCAATCAGTCCAGGCCCTCCTTTGCCGCGCTGTGACTCAGGTCAATGCCGACCTCCTCCAGCTGCTACCGGCCCTGAAGCTCGTGGTGACTGTCAGCACCGGCGCCAATCACGTGGACAAAGCGGAGTGCCGGCGGCGTGGGATTGCTGTGGCCAATGCCACAAATGCGTACTCAGAAGATGTTGCTGATATTGCTGTTGGGTTGTTCATCGATGTGAATCGGAGAATTTCAGCAGCTGATTGGTATGTGAGACGTGGGCTTTGGTCTAGCAAAGGAGACTATCCTCTCGGTTCCAAGATAGGTGGAAAGCGTGTAGGTATTGTTGGATTGGGAAGCATTGGCTATGAAGTTGGCAAAAGACTTGAGGCCTTTGGCTGCAAGATCTCATACAACTCAAGGAGTGAAAGGCCATCTTTGTCATACCCCTTCTATTCCAATGTCTGTGAGCTTGCAGCTAATAATGATGCTCTCATCATCTGTTGTGCATTGACAGACCAAACCCGCCACATGATCAACAAGGAAGTCTTATCTGCATTGGGAAAAGATGGAGTGATAGTTAATATAGGGCGTGGGGCAATTATTGATGAGAAAGAATTGGTCAAGTGTTTGGTGAATGGAGAGATCAGAGGTGCTGGTTTGGATGTGTTTGAGAATGAGCCTCATATACCGAAAGAGCTTGTGGCAATGGACAATGTTGTTCTGTCTCCACATAAGTCTGTTTATACCCTAGAGTCTTTCAAGAAAGGAAGTGAACTAATGATCGCCAATTTGGAAGCTTTCTTCTCCAATAAACCGTTGATTACTCCAGTCTATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

324

Amino Acids

35.39

Weight (kDa)

6.95

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 47 - 321 1.3e-21 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 118 - 288 3.9e-49 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 254
AccB7I CCANNNNNTGG 1 cut(s) 965
AccII CGCG 1 cut(s) 180
AciI CCGC 4 cut(s) 178, 275, 286, 655
AcoI YGGCCR 1 cut(s) 303
AcsI RAATTY 1 cut(s) 378
AcuI CTGAAG 1 cut(s) 248
AcvI CACGTG 1 cut(s) 265
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 1 cut(s) 323
AfiI CCNNNNNNNGG 2 cut(s) 109, 965
AgsI TTSAA 2 cut(s) 94, 892
AjiI CACGTC 1 cut(s) 407
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 8 cut(s) 22, 214, 232, 389, 601, 608, 829, 929
AluI AGCT 8 cut(s) 22, 214, 232, 389, 601, 608, 829, 929
Alw26I GTCTC 3 cut(s) 397, 423, 861
AlwNI CAGNNNCTG 1 cut(s) 389
AoxI GGCC 6 cut(s) 54, 165, 222, 303, 519, 560
ApeKI GCWGC 4 cut(s) 214, 386, 528, 605
ApoI RAATTY 1 cut(s) 378
ArsI GACNNNNNNTTYG 2 cut(s) 396, 428
AspLEI GCGC 2 cut(s) 182, 257
AspS9I GGNCC 4 cut(s) 4, 130, 166, 223
AsuHPI GGTGA 5 cut(s) 73, 108, 133, 250, 775
AsuII TTCGAA 1 cut(s) 69
AvaII GGWCC 2 cut(s) 4, 130
BalI TGGCCA 1 cut(s) 305
BanI GGYRCC 1 cut(s) 254
BauI CACGAG 1 cut(s) 233
BbrPI CACGTG 1 cut(s) 265
BbvI GCAGC 4 cut(s) 201, 398, 515, 617
BccI CCATC 2 cut(s) 571, 692
BciT130I CCWGG 1 cut(s) 164
BclI TGATCA 2 cut(s) 36, 663
BcoDI GTCTC 3 cut(s) 397, 423, 861
BfaI CTAG 2 cut(s) 420, 881
BfoI RGCGCY 1 cut(s) 258
BglII AGATCT 1 cut(s) 534
BisI GCNGC 6 cut(s) 178, 215, 287, 387, 529, 606
BlsI GCNGC 6 cut(s) 179, 216, 288, 388, 530, 607
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 2 cut(s) 4, 130
BmgBI CACGTC 1 cut(s) 407
BmgT120I GGNCC 4 cut(s) 4, 130, 166, 223
BmiI GGNNCC 2 cut(s) 256, 445
BmrFI CCNGG 1 cut(s) 164
BmsI GCATC 1 cut(s) 607
BoxI GACNNNNGTC 1 cut(s) 191
BpmI CTGGAG 2 cut(s) 194, 942
Bpu14I TTCGAA 1 cut(s) 69
BpuEI CTTGAG 2 cut(s) 532, 536
Bsa29I ATCGAT 1 cut(s) 363
BsaAI YACGTR 1 cut(s) 265
BsaBI GATNNNNATC 1 cut(s) 369
BsaHI GRCGYC 1 cut(s) 255
BsaXI ACNNNNNCTCC 2 cut(s) 693, 723
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 965
Bse118I RCCGGY 4 cut(s) 52, 220, 251, 282
Bse1I ACTGG 2 cut(s) 133, 959
Bse3DI GCAATG 1 cut(s) 843
Bse8I GATNNNNATC 1 cut(s) 369
BseBI CCWGG 1 cut(s) 164
BseCI ATCGAT 1 cut(s) 363
BseGI GGATG 2 cut(s) 96, 799
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 2 cut(s) 109, 965
BseMI GCAATG 1 cut(s) 843
BseMII CTCAG 2 cut(s) 203, 339
BseNI ACTGG 2 cut(s) 133, 959
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 4 cut(s) 201, 398, 515, 617
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 6 cut(s) 56, 167, 224, 305, 521, 562
BshNI GGYRCC 1 cut(s) 254
BshVI ATCGAT 1 cut(s) 363
BsiSI CCGG 4 cut(s) 53, 221, 252, 283
BslI CCNNNNNNNGG 2 cut(s) 109, 965
BsmAI GTCTC 3 cut(s) 397, 423, 861
BsmBI CGTCTC 1 cut(s) 397
BsnI GGCC 6 cut(s) 56, 167, 224, 305, 521, 562
Bsp119I TTCGAA 1 cut(s) 69
Bsp143I GATC 5 cut(s) 36, 534, 663, 774, 912
BspACI CCGC 4 cut(s) 178, 275, 286, 655
BspANI GGCC 6 cut(s) 56, 167, 224, 305, 521, 562
BspCNI CTCAG 2 cut(s) 202, 338
BspDI ATCGAT 1 cut(s) 363
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 256, 445
BspT104I TTCGAA 1 cut(s) 69
BspT107I GGYRCC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 843
BsrFI RCCGGY 4 cut(s) 52, 220, 251, 282
BsrI ACTGG 2 cut(s) 133, 959
BssAI RCCGGY 4 cut(s) 52, 220, 251, 282
BssMI GATC 5 cut(s) 36, 534, 663, 774, 912
BssNI GRCGYC 1 cut(s) 255
BssSI CACGAG 1 cut(s) 233
Bst2BI CACGAG 1 cut(s) 233
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 2 cut(s) 244, 948
BstACI GRCGYC 1 cut(s) 255
BstBAI YACGTR 1 cut(s) 265
BstBI TTCGAA 1 cut(s) 69
BstC8I GCNNGC 2 cut(s) 284, 603
BstDEI CTNAG 2 cut(s) 189, 325
BstF5I GGATG 2 cut(s) 96, 799
BstFNI CGCG 1 cut(s) 180
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 2 cut(s) 182, 257
BstKTI GATC 5 cut(s) 39, 537, 666, 777, 915
BstMAI GTCTC 3 cut(s) 397, 423, 861
BstMBI GATC 5 cut(s) 36, 534, 663, 774, 912
BstMWI GCNNNNNNNGC 2 cut(s) 317, 344
BstNI CCWGG 1 cut(s) 164
BstPAI GACNNNNGTC 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 162
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 4 cut(s) 201, 398, 515, 617
BstX2I RGATCY 1 cut(s) 534
BstXI CCANNNNNNTGG 1 cut(s) 265
BstYI RGATCY 1 cut(s) 534
Bsu15I ATCGAT 1 cut(s) 363
BsuRI GGCC 6 cut(s) 56, 167, 224, 305, 521, 562
BsuTUI ATCGAT 1 cut(s) 363
BtrI CACGTC 1 cut(s) 407
BtsCI GGATG 2 cut(s) 96, 799
Cac8I GCNNGC 2 cut(s) 284, 603
CaiI CAGNNNCTG 1 cut(s) 389
CfoI GCGC 2 cut(s) 182, 257
Cfr10I RCCGGY 4 cut(s) 52, 220, 251, 282
Cfr13I GGNCC 4 cut(s) 4, 130, 166, 223
ClaI ATCGAT 1 cut(s) 363
Csp6I GTAC 1 cut(s) 322
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviAII CATG 2 cut(s) 108, 661
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 2 cut(s) 189, 325
DinI GGCGCC 1 cut(s) 256
DpnI GATC 5 cut(s) 38, 536, 665, 776, 914
DpnII GATC 5 cut(s) 36, 534, 663, 774, 912
EaeI YGGCCR 1 cut(s) 303
Eco147I AGGCCT 1 cut(s) 521
Eco47I GGWCC 2 cut(s) 4, 130
Eco57I CTGAAG 1 cut(s) 248
Eco72I CACGTG 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 162
EgeI GGCGCC 1 cut(s) 256
EheI GGCGCC 1 cut(s) 256
Esp3I CGTCTC 1 cut(s) 397
FaeI CATG 2 cut(s) 111, 664
FalI AAGNNNNNCTT 4 cut(s) 550, 582, 662, 694
FatI CATG 2 cut(s) 107, 660
FauI CCCGC 1 cut(s) 662
FbaI TGATCA 2 cut(s) 36, 663
Fnu4HI GCNGC 6 cut(s) 178, 215, 287, 387, 529, 606
FokI GGATG 2 cut(s) 83, 806
Fsp4HI GCNGC 6 cut(s) 178, 215, 287, 387, 529, 606
FspBI CTAG 2 cut(s) 420, 881
GlaI GCGC 2 cut(s) 181, 256
GluI GCNGC 6 cut(s) 178, 215, 287, 387, 529, 606
GsuI CTGGAG 2 cut(s) 194, 942
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 6 cut(s) 56, 167, 224, 305, 521, 562
HapII CCGG 4 cut(s) 53, 221, 252, 283
HhaI GCGC 2 cut(s) 182, 257
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 2 cut(s) 111, 664
Hin6I GCGC 2 cut(s) 180, 255
HinP1I GCGC 2 cut(s) 180, 255
HindIII AAGCTT 1 cut(s) 927
HinfI GANTC 5 cut(s) 71, 113, 187, 370, 884
HpaII CCGG 4 cut(s) 53, 221, 252, 283
HphI GGTGA 5 cut(s) 73, 108, 133, 250, 775
Hpy166II GTNNAC 2 cut(s) 268, 905
Hpy188I TCNGA 5 cut(s) 76, 104, 328, 375, 779
Hpy188III TCNNGA 3 cut(s) 34, 128, 892
Hpy8I GTNNAC 2 cut(s) 268, 905
HpyAV CCTTC 2 cut(s) 67, 589
HpyCH4III ACNGT 2 cut(s) 244, 948
HpyCH4IV ACGT 2 cut(s) 264, 406
HpyCH4V TGCA 5 cut(s) 16, 531, 605, 638, 686
HpyF10VI GCNNNNNNNGC 2 cut(s) 317, 344
HpyF3I CTNAG 2 cut(s) 189, 325
HpySE526I ACGT 2 cut(s) 264, 406
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 2 cut(s) 111, 664
HspAI GCGC 2 cut(s) 180, 255
KasI GGCGCC 1 cut(s) 254
KroI GCCGGC 1 cut(s) 282
KroNI GCCGGC 1 cut(s) 284
Ksp22I TGATCA 2 cut(s) 36, 663
Kzo9I GATC 5 cut(s) 36, 534, 663, 774, 912
LmnI GCTCC 1 cut(s) 27
Lsp1109I GCAGC 4 cut(s) 201, 398, 515, 617
LweI GCATC 1 cut(s) 607
MaeI CTAG 2 cut(s) 420, 881
MaeII ACGT 2 cut(s) 264, 406
MaeIII GTNAC 2 cut(s) 184, 238
MalI GATC 5 cut(s) 38, 536, 665, 776, 914
MboI GATC 5 cut(s) 36, 534, 663, 774, 912
MboII GAAGA 2 cut(s) 341, 925
MflI RGATCY 1 cut(s) 534
MlsI TGGCCA 1 cut(s) 305
MluCI AATT 4 cut(s) 378, 729, 746, 919
MluNI TGGCCA 1 cut(s) 305
Mly113I GGCGCC 1 cut(s) 255
MlyI GAGTC 2 cut(s) 181, 893
MmeI TCCRAC 2 cut(s) 99, 457
Mox20I TGGCCA 1 cut(s) 305
MroNI GCCGGC 1 cut(s) 282
MscI TGGCCA 1 cut(s) 305
MseI TTAA 1 cut(s) 711
MslI CAYNNNNRTG 1 cut(s) 365
Msp20I TGGCCA 1 cut(s) 305
MspA1I CMGCKG 2 cut(s) 214, 389
MspI CCGG 4 cut(s) 53, 221, 252, 283
MspR9I CCNGG 1 cut(s) 164
MvaI CCWGG 1 cut(s) 164
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 2 cut(s) 317, 344
NaeI GCCGGC 1 cut(s) 284
NarI GGCGCC 1 cut(s) 255
NdeII GATC 5 cut(s) 36, 534, 663, 774, 912
NgoMIV GCCGGC 1 cut(s) 282
NlaIII CATG 2 cut(s) 111, 664
NlaIV GGNNCC 2 cut(s) 256, 445
NmuCI GTSAC 2 cut(s) 184, 238
NspV TTCGAA 1 cut(s) 69
PceI AGGCCT 1 cut(s) 521
PdiI GCCGGC 1 cut(s) 284
PfeI GAWTC 3 cut(s) 71, 113, 370
PflMI CCANNNNNTGG 1 cut(s) 965
PkrI GCNGC 6 cut(s) 179, 216, 288, 388, 530, 607
PleI GAGTC 2 cut(s) 181, 892
PluTI GGCGCC 1 cut(s) 258
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 2 cut(s) 181, 892
Ppu21I YACGTR 1 cut(s) 265
PshAI GACNNNNGTC 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 162
PspCI CACGTG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 2 cut(s) 256, 445
PspPI GGNCC 4 cut(s) 4, 130, 166, 223
PstNI CAGNNNCTG 1 cut(s) 389
PsuI RGATCY 1 cut(s) 534
PvuII CAGCTG 2 cut(s) 214, 389
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
RseI CAYNNNNRTG 1 cut(s) 365
SaqAI TTAA 1 cut(s) 711
SatI GCNGC 6 cut(s) 178, 215, 287, 387, 529, 606
Sau3AI GATC 5 cut(s) 36, 534, 663, 774, 912
Sau96I GGNCC 4 cut(s) 4, 130, 166, 223
SchI GAGTC 2 cut(s) 181, 893
ScrFI CCNGG 1 cut(s) 164
SfaNI GCATC 1 cut(s) 607
SfoI GGCGCC 1 cut(s) 256
SfuI TTCGAA 1 cut(s) 69
SgrAI CRCCGGYG 1 cut(s) 251
SinI GGWCC 2 cut(s) 4, 130
SmiMI CAYNNNNRTG 1 cut(s) 365
SmlI CTYRAG 2 cut(s) 515, 547
SmoI CTYRAG 2 cut(s) 515, 547
Sse9I AATT 4 cut(s) 378, 729, 746, 919
SseBI AGGCCT 1 cut(s) 521
SsiI CCGC 4 cut(s) 178, 275, 286, 655
SspDI GGCGCC 1 cut(s) 254
SspMI CTAG 2 cut(s) 420, 881
StuI AGGCCT 1 cut(s) 521
StyD4I CCNGG 1 cut(s) 162
TaaI ACNGT 2 cut(s) 244, 948
TaiI ACGT 2 cut(s) 267, 409
TaqI TCGA 2 cut(s) 69, 363
TasI AATT 4 cut(s) 378, 729, 746, 919
TauI GCSGC 2 cut(s) 180, 289
TfiI GAWTC 3 cut(s) 71, 113, 370
Tru1I TTAA 1 cut(s) 711
Tru9I TTAA 1 cut(s) 711
TseFI GTSAC 2 cut(s) 184, 238
TseI GCWGC 4 cut(s) 214, 386, 528, 605
Tsp45I GTSAC 2 cut(s) 184, 238
TspDTI ATGAA 3 cut(s) 49, 349, 513
Van91I CCANNNNNTGG 1 cut(s) 965
VpaK11BI GGWCC 2 cut(s) 4, 130
XapI RAATTY 1 cut(s) 378
XspI CTAG 2 cut(s) 420, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.