RH1DG231000

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0000446
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG231000.1

Sequence Viewer

Length: 969 bp
ATGGCCCTATTGCTAAACCTCCCAGATGTCCTGATCATCCAACCACCACCAGCCTTCATAACTTTCGAATCCGACTTCACCCAGAAGTTCAACATCCTCCGAGCATGGGAATCGCCACTCCCTCTGGACCAGTTCCTCACCACCCACGCCCAATCAGTCCATGCCCTCCTCTGCCGCGCCGAGACTCCGATCAACGCCGACGTCCTCCGGCTGCTACCGGCCCTGAAGATCGTGGTGTCCGCCAGCACCGGCGCCGACCAAGTGGACAAAGCCGAGTGCCGGCGGCGTGGGATTGCGGTGACCAACGCAGGAAATGCGTACGCGGAAGATGTTGCTGATATCGCTGTTGGGTTGTTCATCGATGTGTTTCGGAAGATCTCAGCAGCTGATTGGTATGTGAGAAAAGGGCTTTGGAGTAGCAAAGGAGATTATCCTCTTGGTTCCAAGATAGGTGGAAAGCGAGTTGGTATTGTTGGATTGGGAAGCATTGGCTATGAAGTTGCCAAAAGACTTGAGGCCTTTGGCTGCAAGATCTCATACAACTCAAGGAGTGAAAGGCCATCTTTGTCATACCCCTTCTATTCCAATGTCTGTGAGCTTGCAGCTAATAATGATGCTCTCATTATCTGTTGTGCATTGACAGACCAAACCCGCCACATGATCAACAAGGAAGTCTTATCTGCATTGGGAAAAGATGGAGTGATAGTTAATATAGGGCGTGGGGCAATTATTGATGAGAAAGAATTGGTCAAGTGTTTGGTGAATGGAGAGATCAGAGGTGCTGGTTTGGATGTGTTTGAGAATGAGCCTCATATACCGAAAGACCTTGTGGCAATGGACAATGTTGTTCTGTCTCCACATAGGGCTGTTTATACAGTAGAGTCTGTCAAGAAAGGAAGTGAAGGAATGATCGCCAATTTGGAAGCTTTCTTCTCCAATAAACCGTTGATTACTCCAGTCTATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

322

Amino Acids

35.02

Weight (kDa)

6.24

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 46 - 320 3.7e-22 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 117 - 289 3.9e-50 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 251
AccB7I CCANNNNNTGG 1 cut(s) 962
AccII CGCG 2 cut(s) 177, 323
AciI CCGC 6 cut(s) 175, 240, 283, 296, 323, 652
AcuI CTGAAG 1 cut(s) 245
AcyI GRCGYC 2 cut(s) 201, 252
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 3 cut(s) 106, 279, 962
AgsI TTSAA 1 cut(s) 91
AjuI GAANNNNNNNTTGG 2 cut(s) 394, 426
AluBI AGCT 4 cut(s) 386, 598, 605, 926
AluI AGCT 4 cut(s) 386, 598, 605, 926
Alw26I GTCTC 2 cut(s) 176, 858
AlwNI CAGNNNCTG 1 cut(s) 386
AoxI GGCC 4 cut(s) 3, 219, 516, 557
ApeKI GCWGC 4 cut(s) 211, 383, 525, 602
AspLEI GCGC 2 cut(s) 179, 254
AspS9I GGNCC 3 cut(s) 4, 127, 220
AsuHPI GGTGA 4 cut(s) 70, 130, 310, 772
AsuII TTCGAA 1 cut(s) 66
AvaII GGWCC 1 cut(s) 127
BanI GGYRCC 1 cut(s) 251
BbvI GCAGC 4 cut(s) 198, 395, 512, 614
BccI CCATC 2 cut(s) 568, 689
BcgI CGANNNNNNTGC 2 cut(s) 93, 127
BclI TGATCA 2 cut(s) 33, 660
BcoDI GTCTC 2 cut(s) 176, 858
BfoI RGCGCY 1 cut(s) 255
BglII AGATCT 2 cut(s) 375, 531
BisI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
BlsI GCNGC 6 cut(s) 176, 213, 285, 385, 527, 604
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 3 cut(s) 4, 127, 220
BmiI GGNNCC 2 cut(s) 253, 442
BmsI GCATC 1 cut(s) 604
BpmI CTGGAG 1 cut(s) 939
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 2 cut(s) 529, 533
Bsa29I ATCGAT 1 cut(s) 360
BsaHI GRCGYC 2 cut(s) 201, 252
BsaXI ACNNNNNCTCC 2 cut(s) 690, 720
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 279, 962
Bse118I RCCGGY 3 cut(s) 217, 248, 279
Bse1I ACTGG 2 cut(s) 130, 956
Bse3DI GCAATG 1 cut(s) 840
BseCI ATCGAT 1 cut(s) 360
BseGI GGATG 3 cut(s) 36, 93, 796
BseLI CCNNNNNNNGG 3 cut(s) 106, 279, 962
BseMI GCAATG 1 cut(s) 840
BseMII CTCAG 1 cut(s) 393
BseNI ACTGG 2 cut(s) 130, 956
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 4 cut(s) 198, 395, 512, 614
Bsh1236I CGCG 2 cut(s) 177, 323
BshFI GGCC 4 cut(s) 5, 221, 518, 559
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 360
BsiSI CCGG 4 cut(s) 208, 218, 249, 280
BsiWI CGTACG 1 cut(s) 318
BslI CCNNNNNNNGG 3 cut(s) 106, 279, 962
BsmAI GTCTC 2 cut(s) 176, 858
BsnI GGCC 4 cut(s) 5, 221, 518, 559
Bsp119I TTCGAA 1 cut(s) 66
Bsp143I GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 771, 909
BspACI CCGC 6 cut(s) 175, 240, 283, 296, 323, 652
BspANI GGCC 4 cut(s) 5, 221, 518, 559
BspCNI CTCAG 1 cut(s) 392
BspDI ATCGAT 1 cut(s) 360
BspFNI CGCG 2 cut(s) 177, 323
BspLI GGNNCC 2 cut(s) 253, 442
BspT104I TTCGAA 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 840
BsrFI RCCGGY 3 cut(s) 217, 248, 279
BsrI ACTGG 2 cut(s) 130, 956
BssAI RCCGGY 3 cut(s) 217, 248, 279
BssMI GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 771, 909
BssNI GRCGYC 2 cut(s) 201, 252
Bst4CI ACNGT 2 cut(s) 877, 945
BstACI GRCGYC 2 cut(s) 201, 252
BstAPI GCANNNNNTGC 1 cut(s) 314
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 3 cut(s) 244, 281, 600
BstDEI CTNAG 1 cut(s) 379
BstEII GGTNACC 1 cut(s) 298
BstF5I GGATG 3 cut(s) 36, 93, 796
BstFNI CGCG 2 cut(s) 177, 323
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 2 cut(s) 179, 254
BstKTI GATC 8 cut(s) 36, 192, 231, 378, 534, 663, 774, 912
BstMAI GTCTC 2 cut(s) 176, 858
BstMBI GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 771, 909
BstMWI GCNNNNNNNGC 2 cut(s) 314, 341
BstPI GGTNACC 1 cut(s) 298
BstUI CGCG 2 cut(s) 177, 323
BstV1I GCAGC 4 cut(s) 198, 395, 512, 614
BstX2I RGATCY 2 cut(s) 375, 531
BstYI RGATCY 2 cut(s) 375, 531
Bsu15I ATCGAT 1 cut(s) 360
BsuRI GGCC 4 cut(s) 5, 221, 518, 559
BsuTUI ATCGAT 1 cut(s) 360
BtsCI GGATG 3 cut(s) 36, 93, 796
Cac8I GCNNGC 3 cut(s) 244, 281, 600
CaiI CAGNNNCTG 1 cut(s) 386
CfoI GCGC 2 cut(s) 179, 254
Cfr10I RCCGGY 3 cut(s) 217, 248, 279
Cfr13I GGNCC 3 cut(s) 4, 127, 220
ClaI ATCGAT 1 cut(s) 360
Csp6I GTAC 1 cut(s) 319
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 3 cut(s) 105, 161, 658
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 1 cut(s) 379
DinI GGCGCC 1 cut(s) 253
DpnI GATC 8 cut(s) 35, 191, 230, 377, 533, 662, 773, 911
DpnII GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 771, 909
EciI GGCGGA 1 cut(s) 229
Eco147I AGGCCT 1 cut(s) 518
Eco32I GATATC 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 245
Eco91I GGTNACC 1 cut(s) 298
EcoO65I GGTNACC 1 cut(s) 298
EcoRV GATATC 1 cut(s) 340
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
FaeI CATG 3 cut(s) 108, 164, 661
FalI AAGNNNNNCTT 4 cut(s) 547, 579, 659, 691
FatI CATG 3 cut(s) 104, 160, 657
FauI CCCGC 1 cut(s) 659
FbaI TGATCA 2 cut(s) 33, 660
Fnu4HI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
FokI GGATG 3 cut(s) 23, 80, 803
Fsp4HI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
GlaI GCGC 2 cut(s) 178, 253
GluI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
GsuI CTGGAG 1 cut(s) 939
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 4 cut(s) 5, 221, 518, 559
HapII CCGG 4 cut(s) 208, 218, 249, 280
HhaI GCGC 2 cut(s) 179, 254
Hin1I GRCGYC 2 cut(s) 201, 252
Hin1II CATG 3 cut(s) 108, 164, 661
Hin6I GCGC 2 cut(s) 177, 252
HinP1I GCGC 2 cut(s) 177, 252
HindIII AAGCTT 1 cut(s) 924
HinfI GANTC 4 cut(s) 68, 110, 184, 881
HpaII CCGG 4 cut(s) 208, 218, 249, 280
HphI GGTGA 4 cut(s) 70, 130, 310, 772
Hpy166II GTNNAC 1 cut(s) 265
Hpy188I TCNGA 5 cut(s) 73, 101, 189, 372, 776
Hpy188III TCNNGA 3 cut(s) 31, 125, 889
Hpy8I GTNNAC 1 cut(s) 265
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 3 cut(s) 64, 586, 896
HpyCH4III ACNGT 2 cut(s) 877, 945
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 4 cut(s) 528, 602, 635, 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 314, 341
HpyF3I CTNAG 1 cut(s) 379
HpySE526I ACGT 1 cut(s) 201
Hsp92I GRCGYC 2 cut(s) 201, 252
Hsp92II CATG 3 cut(s) 108, 164, 661
HspAI GCGC 2 cut(s) 177, 252
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 279
KroNI GCCGGC 1 cut(s) 281
Ksp22I TGATCA 2 cut(s) 33, 660
Kzo9I GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 771, 909
Lsp1109I GCAGC 4 cut(s) 198, 395, 512, 614
LweI GCATC 1 cut(s) 604
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 298
MalI GATC 8 cut(s) 35, 191, 230, 377, 533, 662, 773, 911
MboI GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 771, 909
MboII GAAGA 4 cut(s) 238, 338, 385, 922
MflI RGATCY 2 cut(s) 375, 531
MluCI AATT 3 cut(s) 726, 743, 916
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 2 cut(s) 178, 890
MmeI TCCRAC 3 cut(s) 64, 96, 454
MroNI GCCGGC 1 cut(s) 279
MseI TTAA 1 cut(s) 708
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 4 cut(s) 208, 218, 249, 280
MvnI CGCG 2 cut(s) 177, 323
MwoI GCNNNNNNNGC 2 cut(s) 314, 341
NaeI GCCGGC 1 cut(s) 281
NarI GGCGCC 1 cut(s) 252
NdeII GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 771, 909
NgoMIV GCCGGC 1 cut(s) 279
NlaIII CATG 3 cut(s) 108, 164, 661
NlaIV GGNNCC 2 cut(s) 253, 442
NmeAIII GCCGAG 2 cut(s) 205, 298
NmuCI GTSAC 1 cut(s) 298
NspV TTCGAA 1 cut(s) 66
PceI AGGCCT 1 cut(s) 518
PdiI GCCGGC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 68, 110
Pfl23II CGTACG 1 cut(s) 318
PflMI CCANNNNNTGG 1 cut(s) 962
PkrI GCNGC 6 cut(s) 176, 213, 285, 385, 527, 604
PleI GAGTC 2 cut(s) 178, 889
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 2 cut(s) 178, 889
PspEI GGTNACC 1 cut(s) 298
PspLI CGTACG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 253, 442
PspPI GGNCC 3 cut(s) 4, 127, 220
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 2 cut(s) 375, 531
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
RseI CAYNNNNRTG 1 cut(s) 362
SaqAI TTAA 1 cut(s) 708
SatI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
Sau3AI GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 771, 909
Sau96I GGNCC 3 cut(s) 4, 127, 220
SchI GAGTC 2 cut(s) 178, 890
SetI ASST 9 cut(s) 21, 204, 388, 454, 600, 607, 781, 828, 928
SfaNI GCATC 1 cut(s) 604
SfoI GGCGCC 1 cut(s) 253
SfuI TTCGAA 1 cut(s) 66
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 512, 544
SmoI CTYRAG 2 cut(s) 512, 544
Sse9I AATT 3 cut(s) 726, 743, 916
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 6 cut(s) 175, 240, 283, 296, 323, 652
SspDI GGCGCC 1 cut(s) 251
StuI AGGCCT 1 cut(s) 518
TaaI ACNGT 2 cut(s) 877, 945
TaiI ACGT 1 cut(s) 204
TaqI TCGA 2 cut(s) 66, 360
TasI AATT 3 cut(s) 726, 743, 916
TauI GCSGC 2 cut(s) 177, 286
TfiI GAWTC 2 cut(s) 68, 110
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 4 cut(s) 211, 383, 525, 602
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 3 cut(s) 46, 346, 510
Van91I CCANNNNNTGG 1 cut(s) 962
VpaK11BI GGWCC 1 cut(s) 127
ZraI GACGTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.