Rw1G020340

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000446
Physical Location & Seq
Forward (+)
43982727 .. 43984335
1609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G020340.1

Sequence Viewer

Length: 972 bp
ATGGCCCTATTGCCAAACCTCCCAGATGTCCTGATCATCCAACCACCACCAGCCTTCATATCTTTCGAATCCGACTTCACCCAGAAGTTCAACATCCTCCGCGCATGGGAGTCGCCACTCCCTCTGGACCAGTTCCTCACCACCCACGCCCAATCAGTCCATGCCCTCCTCTGCCGCGCCGAGACTCCGATCAACGCCGACGTCCTCCGGCTGCTACCGGCCCTGAAGATCGTGGTGTCCGCCAGCACCGGCGCCGACCAAGTGGACAAAGCCGAGTGCCGGCGGCGTGGGATTGCGGTGACCAACGCAGGAAATGCGTACGCGGAAGATGTTGCTGATATCGCTGTTGGGTTGTTCATCGATGTGTTTCGGAAGATCTCAGCAGCTGATTGGTATGTGAGAAAAGGGCTTTGGAGTAGCAGTGGAGATTATCCTCTTGGTTCCAAGATAGGTGGAAAGCGAGTTGGTGTTGTTGGATTGGGAAGCATTGGCTATGAAGTTGCCAAAAGACTCGAGGCCTTTGGCTGCAAGATCTCATACAACTCAAGAAGCGAAAGGCCGTCTTTGTCATACACCTTCTATTCCAATGTCAGTAAACTTGCAGCAGACAGTGACGCTCTCGTAATCTGTTGTGGGTTAACAGACCAGACCCGCCACATGATCAACAAGGAAGTCTTATCTGCATTGGGAAAAGATGGAGTGGTAGTTAATATAGGCCGTGGGGCAATTATTGATGAGAAGGAATTGGTCAAGTGTTTGGTGAATGGAGAGATTAGAGGTGCTGGTTTGGATGTGTATGAGAATGAGCCTCATATACCGAAAGAGCTCGTGGCAATGGACAACGTTGTTCTGTCTCCACATAAGGCTGTTTATACAGTAGAGTCTGTCAAGAAAGGAGGTGAAGTAATGATCGCCAATTTGGAAGCTTTCTTCTCCAATAAACCTTTGGTCACTCCAGTCATATATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

323

Amino Acids

34.96

Weight (kDa)

6.24

Isoelectric Point (pI)

36.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 46 - 320 1.8e-22 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 117 - 289 2.3e-50 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 251
AccII CGCG 3 cut(s) 102, 177, 323
AciI CCGC 7 cut(s) 100, 175, 240, 283, 296, 323, 652
AclI AACGTT 1 cut(s) 843
AcuI CTGAAG 1 cut(s) 245
AcyI GRCGYC 2 cut(s) 201, 252
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 2 cut(s) 106, 279
AgsI TTSAA 1 cut(s) 91
AjuI GAANNNNNNNTTGG 2 cut(s) 394, 426
AluBI AGCT 3 cut(s) 386, 826, 926
AluI AGCT 3 cut(s) 386, 826, 926
Alw21I GWGCWC 1 cut(s) 828
Alw26I GTCTC 2 cut(s) 176, 858
AlwNI CAGNNNCTG 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 5 cut(s) 3, 219, 516, 557, 715
ApeKI GCWGC 4 cut(s) 211, 383, 525, 602
AspLEI GCGC 3 cut(s) 104, 179, 254
AspS9I GGNCC 3 cut(s) 4, 127, 220
AsuHPI GGTGA 5 cut(s) 70, 130, 310, 772, 911
AsuII TTCGAA 1 cut(s) 66
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 1 cut(s) 127
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 1 cut(s) 828
BauI CACGAG 1 cut(s) 827
Bbv12I GWGCWC 1 cut(s) 828
BbvI GCAGC 4 cut(s) 198, 395, 512, 614
BccI CCATC 1 cut(s) 689
BceAI ACGGC 2 cut(s) 544, 702
BcgI CGANNNNNNTGC 2 cut(s) 93, 127
BclI TGATCA 2 cut(s) 33, 660
BcoDI GTCTC 2 cut(s) 176, 858
BfoI RGCGCY 1 cut(s) 255
BglII AGATCT 2 cut(s) 375, 531
BisI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
BlsI GCNGC 6 cut(s) 176, 213, 285, 385, 527, 604
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 3 cut(s) 4, 127, 220
BmiI GGNNCC 2 cut(s) 253, 442
BpmI CTGGAG 1 cut(s) 939
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 529
Bsa29I ATCGAT 1 cut(s) 360
BsaHI GRCGYC 2 cut(s) 201, 252
BsaJI CCNNGG 1 cut(s) 718
BsaXI ACNNNNNCTCC 6 cut(s) 406, 436, 690, 720, 888, 918
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 279
Bse118I RCCGGY 3 cut(s) 217, 248, 279
Bse1I ACTGG 2 cut(s) 130, 956
Bse3DI GCAATG 1 cut(s) 840
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 1 cut(s) 718
BseGI GGATG 3 cut(s) 36, 93, 796
BseLI CCNNNNNNNGG 2 cut(s) 106, 279
BseMI GCAATG 1 cut(s) 840
BseMII CTCAG 1 cut(s) 393
BseNI ACTGG 2 cut(s) 130, 956
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 4 cut(s) 198, 395, 512, 614
Bsh1236I CGCG 3 cut(s) 102, 177, 323
BshFI GGCC 5 cut(s) 5, 221, 518, 559, 717
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 360
BsiHKAI GWGCWC 1 cut(s) 828
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 4 cut(s) 208, 218, 249, 280
BsiWI CGTACG 1 cut(s) 318
BslI CCNNNNNNNGG 2 cut(s) 106, 279
BsmAI GTCTC 2 cut(s) 176, 858
BsnI GGCC 5 cut(s) 5, 221, 518, 559, 717
BsoBI CYCGRG 1 cut(s) 512
Bsp119I TTCGAA 1 cut(s) 66
Bsp1286I GDGCHC 1 cut(s) 828
Bsp143I GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 909
BspACI CCGC 7 cut(s) 100, 175, 240, 283, 296, 323, 652
BspANI GGCC 5 cut(s) 5, 221, 518, 559, 717
BspCNI CTCAG 1 cut(s) 392
BspDI ATCGAT 1 cut(s) 360
BspFNI CGCG 3 cut(s) 102, 177, 323
BspLI GGNNCC 2 cut(s) 253, 442
BspT104I TTCGAA 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 840
BsrFI RCCGGY 3 cut(s) 217, 248, 279
BsrI ACTGG 2 cut(s) 130, 956
BssAI RCCGGY 3 cut(s) 217, 248, 279
BssECI CCNNGG 1 cut(s) 718
BssMI GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 909
BssNI GRCGYC 2 cut(s) 201, 252
BssSI CACGAG 1 cut(s) 827
Bst2BI CACGAG 1 cut(s) 827
Bst4CI ACNGT 2 cut(s) 611, 877
BstACI GRCGYC 2 cut(s) 201, 252
BstAPI GCANNNNNTGC 1 cut(s) 314
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 2 cut(s) 244, 281
BstDEI CTNAG 1 cut(s) 379
BstDSI CCRYGG 1 cut(s) 718
BstEII GGTNACC 1 cut(s) 298
BstF5I GGATG 3 cut(s) 36, 93, 796
BstFNI CGCG 3 cut(s) 102, 177, 323
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 3 cut(s) 104, 179, 254
BstKTI GATC 7 cut(s) 36, 192, 231, 378, 534, 663, 912
BstMAI GTCTC 2 cut(s) 176, 858
BstMBI GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 909
BstMWI GCNNNNNNNGC 2 cut(s) 314, 341
BstPI GGTNACC 1 cut(s) 298
BstUI CGCG 3 cut(s) 102, 177, 323
BstV1I GCAGC 4 cut(s) 198, 395, 512, 614
BstX2I RGATCY 2 cut(s) 375, 531
BstYI RGATCY 2 cut(s) 375, 531
Bsu15I ATCGAT 1 cut(s) 360
BsuRI GGCC 5 cut(s) 5, 221, 518, 559, 717
BsuTUI ATCGAT 1 cut(s) 360
BtgI CCRYGG 1 cut(s) 718
BtsCI GGATG 3 cut(s) 36, 93, 796
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 2 cut(s) 427, 616
Cac8I GCNNGC 2 cut(s) 244, 281
CaiI CAGNNNCTG 1 cut(s) 386
CfoI GCGC 3 cut(s) 104, 179, 254
Cfr10I RCCGGY 3 cut(s) 217, 248, 279
Cfr13I GGNCC 3 cut(s) 4, 127, 220
ClaI ATCGAT 1 cut(s) 360
CseI GACGC 1 cut(s) 623
Csp6I GTAC 1 cut(s) 319
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 3 cut(s) 105, 161, 658
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 1 cut(s) 379
DinI GGCGCC 1 cut(s) 253
DpnI GATC 7 cut(s) 35, 191, 230, 377, 533, 662, 911
DpnII GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 909
EciI GGCGGA 1 cut(s) 229
Ecl136II GAGCTC 1 cut(s) 826
Eco147I AGGCCT 1 cut(s) 518
Eco24I GRGCYC 1 cut(s) 828
Eco32I GATATC 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 826
Eco57I CTGAAG 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 512
Eco91I GGTNACC 1 cut(s) 298
EcoICRI GAGCTC 1 cut(s) 826
EcoO65I GGTNACC 1 cut(s) 298
EcoRV GATATC 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 828
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
FaeI CATG 3 cut(s) 108, 164, 661
FalI AAGNNNNNCTT 4 cut(s) 547, 579, 659, 691
FatI CATG 3 cut(s) 104, 160, 657
FauI CCCGC 1 cut(s) 659
FbaI TGATCA 2 cut(s) 33, 660
Fnu4HI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
FokI GGATG 3 cut(s) 23, 80, 803
FriOI GRGCYC 1 cut(s) 828
Fsp4HI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
GlaI GCGC 3 cut(s) 103, 178, 253
GluI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
GsuI CTGGAG 1 cut(s) 939
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 5 cut(s) 5, 221, 518, 559, 717
HapII CCGG 4 cut(s) 208, 218, 249, 280
HgaI GACGC 1 cut(s) 623
HhaI GCGC 3 cut(s) 104, 179, 254
Hin1I GRCGYC 2 cut(s) 201, 252
Hin1II CATG 3 cut(s) 108, 164, 661
Hin6I GCGC 3 cut(s) 102, 177, 252
HinP1I GCGC 3 cut(s) 102, 177, 252
HincII GTYRAC 1 cut(s) 639
HindII GTYRAC 1 cut(s) 639
HindIII AAGCTT 1 cut(s) 924
HinfI GANTC 5 cut(s) 68, 110, 184, 510, 881
HpaI GTTAAC 1 cut(s) 639
HpaII CCGG 4 cut(s) 208, 218, 249, 280
HphI GGTGA 5 cut(s) 70, 130, 310, 772, 911
Hpy166II GTNNAC 3 cut(s) 265, 596, 639
Hpy188I TCNGA 3 cut(s) 73, 189, 372
Hpy188III TCNNGA 4 cut(s) 31, 125, 546, 889
Hpy8I GTNNAC 3 cut(s) 265, 596, 639
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 3 cut(s) 64, 586, 733
HpyCH4III ACNGT 2 cut(s) 611, 877
HpyCH4IV ACGT 2 cut(s) 201, 843
HpyCH4V TGCA 3 cut(s) 528, 602, 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 314, 341
HpyF3I CTNAG 1 cut(s) 379
HpySE526I ACGT 2 cut(s) 201, 843
Hsp92I GRCGYC 2 cut(s) 201, 252
Hsp92II CATG 3 cut(s) 108, 164, 661
HspAI GCGC 3 cut(s) 102, 177, 252
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 279
KroNI GCCGGC 1 cut(s) 281
Ksp22I TGATCA 2 cut(s) 33, 660
KspAI GTTAAC 1 cut(s) 639
Kzo9I GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 909
Lsp1109I GCAGC 4 cut(s) 198, 395, 512, 614
MaeII ACGT 2 cut(s) 201, 843
MaeIII GTNAC 3 cut(s) 298, 611, 949
MalI GATC 7 cut(s) 35, 191, 230, 377, 533, 662, 911
MboI GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 909
MboII GAAGA 4 cut(s) 238, 338, 385, 922
MflI RGATCY 2 cut(s) 375, 531
MhlI GDGCHC 1 cut(s) 828
MluCI AATT 3 cut(s) 726, 743, 916
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 4 cut(s) 119, 178, 504, 890
MmeI TCCRAC 3 cut(s) 64, 96, 454
MroNI GCCGGC 1 cut(s) 279
MseI TTAA 2 cut(s) 638, 708
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 4 cut(s) 208, 218, 249, 280
MvnI CGCG 3 cut(s) 102, 177, 323
MwoI GCNNNNNNNGC 2 cut(s) 314, 341
NaeI GCCGGC 1 cut(s) 281
NarI GGCGCC 1 cut(s) 252
NdeII GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 909
NgoMIV GCCGGC 1 cut(s) 279
NlaIII CATG 3 cut(s) 108, 164, 661
NlaIV GGNNCC 2 cut(s) 253, 442
NmeAIII GCCGAG 2 cut(s) 205, 298
NmuCI GTSAC 3 cut(s) 298, 611, 949
NspV TTCGAA 1 cut(s) 66
PaeR7I CTCGAG 1 cut(s) 512
PceI AGGCCT 1 cut(s) 518
PdiI GCCGGC 1 cut(s) 281
PfeI GAWTC 1 cut(s) 68
Pfl23II CGTACG 1 cut(s) 318
PkrI GCNGC 6 cut(s) 176, 213, 285, 385, 527, 604
PleI GAGTC 4 cut(s) 118, 178, 504, 889
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 4 cut(s) 118, 178, 504, 889
Psp124BI GAGCTC 1 cut(s) 828
Psp1406I AACGTT 1 cut(s) 843
PspEI GGTNACC 1 cut(s) 298
PspLI CGTACG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 253, 442
PspPI GGNCC 3 cut(s) 4, 127, 220
PspXI VCTCGAGB 1 cut(s) 512
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 2 cut(s) 375, 531
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
RseI CAYNNNNRTG 1 cut(s) 362
SacI GAGCTC 1 cut(s) 828
SaqAI TTAA 2 cut(s) 638, 708
SatI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
Sau3AI GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 909
Sau96I GGNCC 3 cut(s) 4, 127, 220
SchI GAGTC 4 cut(s) 119, 178, 504, 890
SduI GDGCHC 1 cut(s) 828
SfoI GGCGCC 1 cut(s) 253
Sfr274I CTCGAG 1 cut(s) 512
SfuI TTCGAA 1 cut(s) 66
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 1 cut(s) 127
SlaI CTCGAG 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 512, 544
SmoI CTYRAG 2 cut(s) 512, 544
Sse9I AATT 3 cut(s) 726, 743, 916
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 7 cut(s) 100, 175, 240, 283, 296, 323, 652
SspDI GGCGCC 1 cut(s) 251
SstI GAGCTC 1 cut(s) 828
StuI AGGCCT 1 cut(s) 518
TaaI ACNGT 2 cut(s) 611, 877
TaiI ACGT 2 cut(s) 204, 846
TaqI TCGA 3 cut(s) 66, 360, 513
TasI AATT 3 cut(s) 726, 743, 916
TauI GCSGC 2 cut(s) 177, 286
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 2 cut(s) 638, 708
Tru9I TTAA 2 cut(s) 638, 708
TscAI CASTG 2 cut(s) 427, 616
TseFI GTSAC 3 cut(s) 298, 611, 949
TseI GCWGC 4 cut(s) 211, 383, 525, 602
Tsp45I GTSAC 3 cut(s) 298, 611, 949
TspDTI ATGAA 3 cut(s) 46, 346, 510
TspRI CASTG 2 cut(s) 427, 616
VpaK11BI GGWCC 1 cut(s) 127
XcmI CCANNNNNNNNNTGG 1 cut(s) 943
XhoI CTCGAG 1 cut(s) 512
ZraI GACGTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.