Prupe.2G150200_v2.0.a1

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000446
Physical Location & Seq
Forward (+)
20537836 .. 20539452
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G150200.1

Sequence Viewer

Length: 975 bp
ATGGTCCAATTCCAATCCCAAGACCTCCCACAACTCCTGATTATTCAACCTCCATTATGCTTGGCAATTGCTGAATCTCAGTTGTCTCAAAAATTCCATCTCCTCAAAGCATGGGAGTCAGAACTCCCTCTAGACCAGTTCTTGACCACCTATGCATGCTCAGTCCAAGCCATGCTTTGCTACCCTTTCACTCAGGTCAATGCAGACCTCCTCCGGCTACTTCCGGCACTCAAACTTGTTGTCAGCCCCACTGCTGGGGTCAACAACATCGATGTGGTCGAGTGCCGGCGGCGTGGAATATCAGTGACCACTTCTGGGAGTGCATTCTCAGAAGATGTTGCTGATACTGCCGTAGGTTTGCTCATTGATGTGCATAGAAGAATCTCAGCAGCAGATCGGTATGTGAAAGGGCTTTGGACTAGCAAAGGAGATTATCCTCTTGGTTCCAAGTTAGGAGGCAAGCGAGTTGGGATTGTTGGATTAGGAAGCATTGGCTCTGAAATTGCAAAAAGACTGGAGGCCTTTGGGTGCATTGTCTCATACAACTCAAGGAGCAAAAATCCATCTTTAACATACCCCTTCTATTCCAGTGTTCATGAACTTGCAGCCAATACTGATGCCCTCATCATCTGTTGTGCATTGACAGACCAAACCCGCCACATGATCAACAAGGAAGTCTTATCTGCACTGGGAAGAGAGGGAGTGATTGTCAACGTTGGACGGGGGGCTATTATTGATGAGAAGGAATTGGTGAGGTTTCTGGTGCATGGAGAGATTGGAGGCGCTGGTTTGGATGTGTTTGAGAATGAGCCTCATGTTCCTGAAGAACTCTTTGCTTTGGACAACGTTGTTCTGTCTCCACATAACGCTGCTTTTACTCCCGAATCTGTAGAGAGTTCGAGTCAAATAGTTATTGCCAATTTGGAAGCTTTCTTCTCAAATGAGCCTTTGGTTACTCCATTCATGGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

325

Amino Acids

35.2

Weight (kDa)

5.06

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 289, 655
AclI AACGTT 2 cut(s) 714, 846
AcsI RAATTY 1 cut(s) 92
AcuI CTGAAG 1 cut(s) 843
AfiI CCNNNNNNNGG 3 cut(s) 254, 255, 315
AgsI TTSAA 1 cut(s) 47
AluBI AGCT 1 cut(s) 929
AluI AGCT 1 cut(s) 929
Alw26I GTCTC 3 cut(s) 90, 541, 861
AoxI GGCC 1 cut(s) 519
ApeKI GCWGC 3 cut(s) 389, 605, 869
ApoI RAATTY 1 cut(s) 92
ArsI GACNNNNNNTTYG 2 cut(s) 225, 257
AspLEI GCGC 1 cut(s) 785
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 1 cut(s) 763
AvaII GGWCC 1 cut(s) 4
BaeI ACNNNNGTAYC 2 cut(s) 336, 369
BbvI GCAGC 3 cut(s) 401, 617, 856
BccI CCATC 2 cut(s) 105, 571
BceAI ACGGC 1 cut(s) 335
BclI TGATCA 1 cut(s) 663
BcoDI GTCTC 3 cut(s) 90, 541, 861
BfaI CTAG 2 cut(s) 131, 420
BfmI CTRYAG 1 cut(s) 888
BfoI RGCGCY 1 cut(s) 786
BisI GCNGC 4 cut(s) 290, 390, 606, 870
BlsI GCNGC 4 cut(s) 291, 391, 607, 871
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 445
BmrI ACTGGG 1 cut(s) 698
BmsI GCATC 1 cut(s) 607
BmuI ACTGGG 1 cut(s) 698
BpmI CTGGAG 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 532
Bsa29I ATCGAT 1 cut(s) 270
BsaXI ACNNNNNCTCC 2 cut(s) 693, 723
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 255, 315
Bse118I RCCGGY 1 cut(s) 285
Bse1I ACTGG 4 cut(s) 136, 519, 588, 693
BseCI ATCGAT 1 cut(s) 270
BseGI GGATG 2 cut(s) 799, 973
BseLI CCNNNNNNNGG 3 cut(s) 254, 255, 315
BseMII CTCAG 5 cut(s) 92, 174, 206, 342, 399
BseNI ACTGG 4 cut(s) 136, 519, 588, 693
BseRI GAGGAG 2 cut(s) 92, 200
BseXI GCAGC 3 cut(s) 401, 617, 856
BseYI CCCAGC 1 cut(s) 254
BsgI GTGCAG 1 cut(s) 669
BshFI GGCC 1 cut(s) 521
BshVI ATCGAT 1 cut(s) 270
BsiSI CCGG 3 cut(s) 214, 224, 286
BslI CCNNNNNNNGG 3 cut(s) 254, 255, 315
BsmAI GTCTC 3 cut(s) 90, 541, 861
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 1 cut(s) 521
Bsp143I GATC 2 cut(s) 394, 663
BspACI CCGC 2 cut(s) 289, 655
BspANI GGCC 1 cut(s) 521
BspCNI CTCAG 5 cut(s) 91, 173, 205, 341, 398
BspDI ATCGAT 1 cut(s) 270
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 1 cut(s) 445
BsrFI RCCGGY 1 cut(s) 285
BsrI ACTGG 4 cut(s) 136, 519, 588, 693
BssAI RCCGGY 1 cut(s) 285
BssMI GATC 2 cut(s) 394, 663
Bst6I CTCTTC 1 cut(s) 688
BstC8I GCNNGC 3 cut(s) 157, 287, 461
BstDEI CTNAG 5 cut(s) 78, 160, 192, 328, 385
BstF5I GGATG 2 cut(s) 799, 973
BstH2I RGCGCY 1 cut(s) 786
BstHHI GCGC 1 cut(s) 785
BstKTI GATC 2 cut(s) 397, 666
BstMAI GTCTC 3 cut(s) 90, 541, 861
BstMBI GATC 2 cut(s) 394, 663
BstMWI GCNNNNNNNGC 1 cut(s) 347
BstNSI RCATGY 1 cut(s) 159
BstSFI CTRYAG 1 cut(s) 888
BstV1I GCAGC 3 cut(s) 401, 617, 856
Bsu15I ATCGAT 1 cut(s) 270
BsuRI GGCC 1 cut(s) 521
BsuTUI ATCGAT 1 cut(s) 270
BtsCI GGATG 2 cut(s) 799, 973
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 4 cut(s) 249, 309, 595, 686
Cac8I GCNNGC 3 cut(s) 157, 287, 461
CciI TCATGA 1 cut(s) 595
CfoI GCGC 1 cut(s) 785
Cfr10I RCCGGY 1 cut(s) 285
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 1 cut(s) 270
CviAII CATG 8 cut(s) 111, 156, 172, 596, 661, 767, 815, 964
DdeI CTNAG 5 cut(s) 78, 160, 192, 328, 385
DpnI GATC 2 cut(s) 396, 665
DpnII GATC 2 cut(s) 394, 663
Eam1104I CTCTTC 1 cut(s) 688
EarI CTCTTC 1 cut(s) 688
Eco147I AGGCCT 1 cut(s) 521
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 843
EcoT22I ATGCAT 1 cut(s) 157
FaeI CATG 8 cut(s) 114, 159, 175, 599, 664, 770, 818, 967
FalI AAGNNNNNCTT 4 cut(s) 159, 191, 662, 694
FatI CATG 8 cut(s) 110, 155, 171, 595, 660, 766, 814, 963
FauI CCCGC 1 cut(s) 662
FbaI TGATCA 1 cut(s) 663
Fnu4HI GCNGC 4 cut(s) 290, 390, 606, 870
FokI GGATG 1 cut(s) 806
Fsp4HI GCNGC 4 cut(s) 290, 390, 606, 870
FspBI CTAG 2 cut(s) 131, 420
GlaI GCGC 1 cut(s) 784
GluI GCNGC 4 cut(s) 290, 390, 606, 870
GsaI CCCAGC 1 cut(s) 258
GsuI CTGGAG 1 cut(s) 536
HaeII RGCGCY 1 cut(s) 786
HaeIII GGCC 1 cut(s) 521
HapII CCGG 3 cut(s) 214, 224, 286
HhaI GCGC 1 cut(s) 785
Hin1II CATG 8 cut(s) 114, 159, 175, 599, 664, 770, 818, 967
Hin6I GCGC 1 cut(s) 783
HinP1I GCGC 1 cut(s) 783
HincII GTYRAC 2 cut(s) 262, 712
HindII GTYRAC 2 cut(s) 262, 712
HindIII AAGCTT 1 cut(s) 927
HinfI GANTC 5 cut(s) 74, 116, 381, 884, 901
HpaII CCGG 3 cut(s) 214, 224, 286
HphI GGTGA 1 cut(s) 763
Hpy166II GTNNAC 2 cut(s) 262, 712
Hpy188I TCNGA 3 cut(s) 121, 331, 499
Hpy188III TCNNGA 6 cut(s) 37, 131, 142, 596, 821, 881
Hpy8I GTNNAC 2 cut(s) 262, 712
HpyAV CCTTC 2 cut(s) 589, 736
HpyCH4IV ACGT 2 cut(s) 714, 846
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpyF3I CTNAG 5 cut(s) 78, 160, 192, 328, 385
HpySE526I ACGT 2 cut(s) 714, 846
Hsp92II CATG 8 cut(s) 114, 159, 175, 599, 664, 770, 818, 967
HspAI GCGC 1 cut(s) 783
KroI GCCGGC 1 cut(s) 285
KroNI GCCGGC 1 cut(s) 287
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 2 cut(s) 394, 663
LmnI GCTCC 1 cut(s) 552
Lsp1109I GCAGC 3 cut(s) 401, 617, 856
LweI GCATC 1 cut(s) 607
MaeI CTAG 2 cut(s) 131, 420
MaeII ACGT 2 cut(s) 714, 846
MaeIII GTNAC 2 cut(s) 304, 952
MalI GATC 2 cut(s) 396, 665
MboI GATC 2 cut(s) 394, 663
MboII GAAGA 5 cut(s) 344, 390, 705, 836, 925
MfeI CAATTG 1 cut(s) 66
MluCI AATT 6 cut(s) 8, 66, 92, 501, 746, 919
MlyI GAGTC 2 cut(s) 125, 910
MmeI TCCRAC 2 cut(s) 457, 697
Mph1103I ATGCAT 1 cut(s) 157
MroNI GCCGGC 1 cut(s) 285
MseI TTAA 1 cut(s) 569
MslI CAYNNNNRTG 2 cut(s) 272, 368
MspI CCGG 3 cut(s) 214, 224, 286
MunI CAATTG 1 cut(s) 66
Mva1269I GAATGC 1 cut(s) 323
MwoI GCNNNNNNNGC 1 cut(s) 347
NaeI GCCGGC 1 cut(s) 287
NdeII GATC 2 cut(s) 394, 663
NgoMIV GCCGGC 1 cut(s) 285
NlaIII CATG 8 cut(s) 114, 159, 175, 599, 664, 770, 818, 967
NlaIV GGNNCC 1 cut(s) 445
NmuCI GTSAC 1 cut(s) 304
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 1 cut(s) 159
PaeI GCATGC 1 cut(s) 159
PagI TCATGA 1 cut(s) 595
PceI AGGCCT 1 cut(s) 521
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 323
PdiI GCCGGC 1 cut(s) 287
PfeI GAWTC 3 cut(s) 74, 381, 884
PkrI GCNGC 4 cut(s) 291, 391, 607, 871
PleI GAGTC 2 cut(s) 124, 909
PpsI GAGTC 2 cut(s) 124, 909
Psp1406I AACGTT 2 cut(s) 714, 846
PspFI CCCAGC 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 445
PspPI GGNCC 1 cut(s) 4
RseI CAYNNNNRTG 2 cut(s) 272, 368
SaqAI TTAA 1 cut(s) 569
SatI GCNGC 4 cut(s) 290, 390, 606, 870
Sau3AI GATC 2 cut(s) 394, 663
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 2 cut(s) 125, 910
SfaNI GCATC 1 cut(s) 607
SfcI CTRYAG 1 cut(s) 888
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 2 cut(s) 272, 368
SmlI CTYRAG 1 cut(s) 547
SmoI CTYRAG 1 cut(s) 547
SphI GCATGC 1 cut(s) 159
Sse9I AATT 6 cut(s) 8, 66, 92, 501, 746, 919
SseBI AGGCCT 1 cut(s) 521
SsiI CCGC 2 cut(s) 289, 655
SspMI CTAG 2 cut(s) 131, 420
StuI AGGCCT 1 cut(s) 521
TaiI ACGT 2 cut(s) 717, 849
TaqI TCGA 3 cut(s) 270, 279, 899
TasI AATT 6 cut(s) 8, 66, 92, 501, 746, 919
TauI GCSGC 1 cut(s) 292
TfiI GAWTC 3 cut(s) 74, 381, 884
Tru1I TTAA 1 cut(s) 569
Tru9I TTAA 1 cut(s) 569
TscAI CASTG 4 cut(s) 256, 309, 595, 693
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 3 cut(s) 389, 605, 869
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 3 cut(s) 584, 612, 952
TspRI CASTG 4 cut(s) 256, 309, 595, 693
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 1 cut(s) 92
XbaI TCTAGA 1 cut(s) 130
XceI RCATGY 1 cut(s) 159
XspI CTAG 2 cut(s) 131, 420
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.