Prupe.2G150000_v2.0.a1

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000446
Physical Location & Seq
Forward (+)
20528604 .. 20530729
2126 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G150000.1

Sequence Viewer

Length: 975 bp
ATGGTCCAATTCCAATCCCAAGACCTCCCACAACTCCTGATTATTCAACCTCCATTATGCTTGGCAATTGCTGAATCTCAGTTGTCTCAAAAATTCCATCTCCTCAAAGCATGGGAGTCAGAACTCCCTCTAGACCAGTTCTTGACCACCTATGCATGCTCAGTCCAAGCCATGCTTTGCTACCCTTTCACTCAGGTCAATGCAGACCTCCTCCGGCTACTTCCGGCACTCAAACTTGTTGTCAGCCCCACTGCTGGGGTCAACAACATCGATGTGGTCGAGTGCCGGCGGCGTGGAATATCAGTGACCACTTCTGGGAGTGCATTCTCAGAAGATGTTGCTGATACTGCCGTAGGTTTGCTCATTGATGTGCATAGAAGAATCTCAGCAGCAGATCGGTATGTGAAAGGGCTTTGGACTAGCAAAGGAGATTATCCTCTTGGTTCCAAGATAGGAGGCAAACGAGTTGGGATTGTTGGCTTGGGAAACATTGGCTTAGAAGTTGCCAAAAGACTTGAGGCCTTTGGCTGCAACATATTGTACAACTCAAGGACAGAAAAGCCATTCGTTTCATACCCTTTCTATTCTGATATCTGTGAACTAGCTGCTAACAGTGACGCCCTTGTCATTTGTTGTGCATTGACTGCTGAAACCCACCACCTGATCAACAAGAAAGTCTCGTTAAAGCTGGGAAGAGATGGCGTGATTGTAAACGTAGGACGCGGAGCTATCATCGATGAGAAGGAAATGGTGCGGTGTTTGGTGAAAAGAGAGATTGGAGGTGCTGGTTTGGATGTGTTTGAGGATGAGCCTGAAGTTCCTGAAGAGCTCTTTGCATTGGACAATGTTGTACTGTCACCACATTATGCTACCTACACACCAGAATGTTTCATGGCTTTGTGTGAACTAGTAGCAGGGAATTTCGAAGCATTCTTCTCAAATAAACCGTTGCTTTCACCGGCTGTGGATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

325

Amino Acids

35.4

Weight (kDa)

5.16

Isoelectric Point (pI)

49.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 623
AccII CGCG 1 cut(s) 723
AciI CCGC 3 cut(s) 289, 723, 754
AcsI RAATTY 2 cut(s) 92, 919
AcuI CTGAAG 2 cut(s) 834, 843
AcyI GRCGYC 1 cut(s) 618
AfaI GTAC 2 cut(s) 542, 852
AfiI CCNNNNNNNGG 3 cut(s) 254, 255, 315
AgsI TTSAA 1 cut(s) 47
AhlI ACTAGT 1 cut(s) 907
AluBI AGCT 4 cut(s) 605, 688, 728, 829
AluI AGCT 4 cut(s) 605, 688, 728, 829
Alw21I GWGCWC 1 cut(s) 831
Alw26I GTCTC 2 cut(s) 90, 682
AoxI GGCC 1 cut(s) 519
ApeKI GCWGC 3 cut(s) 389, 528, 605
ApoI RAATTY 2 cut(s) 92, 919
ArsI GACNNNNNNTTYG 2 cut(s) 225, 257
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 3 cut(s) 775, 849, 948
AsuII TTCGAA 1 cut(s) 924
AvaII GGWCC 1 cut(s) 4
BaeI ACNNNNGTAYC 2 cut(s) 336, 369
BanII GRGCYC 1 cut(s) 831
Bbv12I GWGCWC 1 cut(s) 831
BbvI GCAGC 3 cut(s) 401, 515, 592
BccI CCATC 2 cut(s) 105, 692
BceAI ACGGC 1 cut(s) 335
BclI TGATCA 1 cut(s) 663
BcoDI GTCTC 2 cut(s) 90, 682
BcuI ACTAGT 1 cut(s) 907
BfaI CTAG 4 cut(s) 131, 420, 602, 908
BisI GCNGC 4 cut(s) 290, 390, 529, 606
BlsI GCNGC 4 cut(s) 291, 391, 530, 607
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 445
Bpu14I TTCGAA 1 cut(s) 924
BpuEI CTTGAG 2 cut(s) 532, 536
Bsa29I ATCGAT 2 cut(s) 270, 735
BsaHI GRCGYC 1 cut(s) 618
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 255, 315
Bse118I RCCGGY 2 cut(s) 285, 958
Bse1I ACTGG 1 cut(s) 136
BseCI ATCGAT 2 cut(s) 270, 735
BseGI GGATG 2 cut(s) 799, 811
BseLI CCNNNNNNNGG 3 cut(s) 254, 255, 315
BseMII CTCAG 5 cut(s) 92, 174, 206, 342, 399
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 2 cut(s) 92, 200
BseXI GCAGC 3 cut(s) 401, 515, 592
BseYI CCCAGC 2 cut(s) 254, 688
Bsh1236I CGCG 1 cut(s) 723
BshFI GGCC 1 cut(s) 521
BshVI ATCGAT 2 cut(s) 270, 735
BsiHKAI GWGCWC 1 cut(s) 831
BsiSI CCGG 4 cut(s) 214, 224, 286, 959
BslI CCNNNNNNNGG 3 cut(s) 254, 255, 315
BsmAI GTCTC 2 cut(s) 90, 682
BsmI GAATGC 2 cut(s) 323, 929
BsnI GGCC 1 cut(s) 521
Bsp119I TTCGAA 1 cut(s) 924
Bsp1286I GDGCHC 1 cut(s) 831
Bsp1407I TGTACA 1 cut(s) 540
Bsp143I GATC 2 cut(s) 394, 663
BspACI CCGC 3 cut(s) 289, 723, 754
BspANI GGCC 1 cut(s) 521
BspCNI CTCAG 5 cut(s) 91, 173, 205, 341, 398
BspDI ATCGAT 2 cut(s) 270, 735
BspFNI CGCG 1 cut(s) 723
BspLI GGNNCC 1 cut(s) 445
BspQI GCTCTTC 1 cut(s) 819
BspT104I TTCGAA 1 cut(s) 924
BsrFI RCCGGY 2 cut(s) 285, 958
BsrGI TGTACA 1 cut(s) 540
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 2 cut(s) 285, 958
BssMI GATC 2 cut(s) 394, 663
BssNI GRCGYC 1 cut(s) 618
Bst4CI ACNGT 3 cut(s) 614, 855, 948
Bst6I CTCTTC 2 cut(s) 688, 819
BstACI GRCGYC 1 cut(s) 618
BstAPI GCANNNNNTGC 1 cut(s) 644
BstAUI TGTACA 1 cut(s) 540
BstBI TTCGAA 1 cut(s) 924
BstC8I GCNNGC 2 cut(s) 157, 287
BstDEI CTNAG 6 cut(s) 78, 160, 192, 328, 385, 496
BstF5I GGATG 2 cut(s) 799, 811
BstFNI CGCG 1 cut(s) 723
BstKTI GATC 2 cut(s) 397, 666
BstMAI GTCTC 2 cut(s) 90, 682
BstMBI GATC 2 cut(s) 394, 663
BstMWI GCNNNNNNNGC 2 cut(s) 347, 644
BstNSI RCATGY 1 cut(s) 159
BstUI CGCG 1 cut(s) 723
BstV1I GCAGC 3 cut(s) 401, 515, 592
Bsu15I ATCGAT 2 cut(s) 270, 735
BsuRI GGCC 1 cut(s) 521
BsuTUI ATCGAT 2 cut(s) 270, 735
BtsCI GGATG 2 cut(s) 799, 811
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 3 cut(s) 249, 309, 619
Cac8I GCNNGC 2 cut(s) 157, 287
Cfr10I RCCGGY 2 cut(s) 285, 958
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 2 cut(s) 270, 735
CseI GACGC 2 cut(s) 626, 729
Csp6I GTAC 2 cut(s) 541, 851
CviAII CATG 4 cut(s) 111, 156, 172, 892
CviQI GTAC 2 cut(s) 541, 851
DdeI CTNAG 6 cut(s) 78, 160, 192, 328, 385, 496
DpnI GATC 2 cut(s) 396, 665
DpnII GATC 2 cut(s) 394, 663
DrdI GACNNNNNNGTC 1 cut(s) 623
DseDI GACNNNNNNGTC 1 cut(s) 623
Eam1104I CTCTTC 2 cut(s) 688, 819
EarI CTCTTC 2 cut(s) 688, 819
Ecl136II GAGCTC 1 cut(s) 829
Eco147I AGGCCT 1 cut(s) 521
Eco24I GRGCYC 1 cut(s) 831
Eco32I GATATC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 4
Eco53kI GAGCTC 1 cut(s) 829
Eco57I CTGAAG 2 cut(s) 834, 843
EcoICRI GAGCTC 1 cut(s) 829
EcoRV GATATC 1 cut(s) 592
EcoT22I ATGCAT 1 cut(s) 157
EcoT38I GRGCYC 1 cut(s) 831
FaeI CATG 4 cut(s) 114, 159, 175, 895
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FatI CATG 4 cut(s) 110, 155, 171, 891
FbaI TGATCA 1 cut(s) 663
Fnu4HI GCNGC 4 cut(s) 290, 390, 529, 606
FokI GGATG 2 cut(s) 806, 818
FriOI GRGCYC 1 cut(s) 831
Fsp4HI GCNGC 4 cut(s) 290, 390, 529, 606
FspBI CTAG 4 cut(s) 131, 420, 602, 908
GluI GCNGC 4 cut(s) 290, 390, 529, 606
GsaI CCCAGC 2 cut(s) 258, 692
HaeIII GGCC 1 cut(s) 521
HapII CCGG 4 cut(s) 214, 224, 286, 959
HgaI GACGC 2 cut(s) 626, 729
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 4 cut(s) 114, 159, 175, 895
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 3 cut(s) 74, 116, 381
HpaII CCGG 4 cut(s) 214, 224, 286, 959
HphI GGTGA 3 cut(s) 775, 849, 948
Hpy166II GTNNAC 4 cut(s) 262, 599, 712, 905
Hpy188I TCNGA 3 cut(s) 121, 331, 589
Hpy188III TCNNGA 4 cut(s) 37, 131, 142, 821
Hpy8I GTNNAC 4 cut(s) 262, 599, 712, 905
HpyAV CCTTC 1 cut(s) 736
HpyCH4III ACNGT 3 cut(s) 614, 855, 948
HpyCH4IV ACGT 1 cut(s) 714
HpyCH4V TGCA 7 cut(s) 155, 203, 323, 373, 531, 638, 836
HpyF10VI GCNNNNNNNGC 2 cut(s) 347, 644
HpyF3I CTNAG 6 cut(s) 78, 160, 192, 328, 385, 496
HpySE526I ACGT 1 cut(s) 714
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 4 cut(s) 114, 159, 175, 895
KroI GCCGGC 1 cut(s) 285
KroNI GCCGGC 1 cut(s) 287
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 2 cut(s) 394, 663
LguI GCTCTTC 1 cut(s) 819
LmnI GCTCC 1 cut(s) 725
Lsp1109I GCAGC 3 cut(s) 401, 515, 592
MaeI CTAG 4 cut(s) 131, 420, 602, 908
MaeII ACGT 1 cut(s) 714
MaeIII GTNAC 3 cut(s) 304, 614, 855
MalI GATC 2 cut(s) 396, 665
MboI GATC 2 cut(s) 394, 663
MboII GAAGA 5 cut(s) 344, 390, 705, 836, 925
MfeI CAATTG 1 cut(s) 66
MhlI GDGCHC 1 cut(s) 831
MluCI AATT 5 cut(s) 8, 66, 92, 919, 970
MlyI GAGTC 1 cut(s) 125
Mph1103I ATGCAT 1 cut(s) 157
MroNI GCCGGC 1 cut(s) 285
MseI TTAA 2 cut(s) 683, 973
MslI CAYNNNNRTG 3 cut(s) 272, 368, 883
MspI CCGG 4 cut(s) 214, 224, 286, 959
MunI CAATTG 1 cut(s) 66
Mva1269I GAATGC 2 cut(s) 323, 929
MvnI CGCG 1 cut(s) 723
MwoI GCNNNNNNNGC 2 cut(s) 347, 644
NaeI GCCGGC 1 cut(s) 287
NdeII GATC 2 cut(s) 394, 663
NgoMIV GCCGGC 1 cut(s) 285
NlaIII CATG 4 cut(s) 114, 159, 175, 895
NlaIV GGNNCC 1 cut(s) 445
NmuCI GTSAC 3 cut(s) 304, 614, 855
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 1 cut(s) 159
NspV TTCGAA 1 cut(s) 924
PaeI GCATGC 1 cut(s) 159
PceI AGGCCT 1 cut(s) 521
PciSI GCTCTTC 1 cut(s) 819
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 2 cut(s) 323, 929
PdiI GCCGGC 1 cut(s) 287
PfeI GAWTC 2 cut(s) 74, 381
PkrI GCNGC 4 cut(s) 291, 391, 530, 607
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp124BI GAGCTC 1 cut(s) 831
PspFI CCCAGC 2 cut(s) 254, 688
PspN4I GGNNCC 1 cut(s) 445
PspPI GGNCC 1 cut(s) 4
RsaI GTAC 2 cut(s) 542, 852
RsaNI GTAC 2 cut(s) 541, 851
RseI CAYNNNNRTG 3 cut(s) 272, 368, 883
SacI GAGCTC 1 cut(s) 831
SapI GCTCTTC 1 cut(s) 819
SaqAI TTAA 2 cut(s) 683, 973
SatI GCNGC 4 cut(s) 290, 390, 529, 606
Sau3AI GATC 2 cut(s) 394, 663
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 125
SduI GDGCHC 1 cut(s) 831
SfuI TTCGAA 1 cut(s) 924
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 3 cut(s) 272, 368, 883
SmlI CTYRAG 2 cut(s) 515, 547
SmoI CTYRAG 2 cut(s) 515, 547
SpeI ACTAGT 1 cut(s) 907
SphI GCATGC 1 cut(s) 159
Sse9I AATT 5 cut(s) 8, 66, 92, 919, 970
SseBI AGGCCT 1 cut(s) 521
SsiI CCGC 3 cut(s) 289, 723, 754
SspMI CTAG 4 cut(s) 131, 420, 602, 908
SstI GAGCTC 1 cut(s) 831
StuI AGGCCT 1 cut(s) 521
TaaI ACNGT 3 cut(s) 614, 855, 948
TaiI ACGT 1 cut(s) 717
TaqI TCGA 4 cut(s) 270, 279, 735, 924
TasI AATT 5 cut(s) 8, 66, 92, 919, 970
TatI WGTACW 2 cut(s) 540, 850
TauI GCSGC 1 cut(s) 292
TfiI GAWTC 2 cut(s) 74, 381
Tru1I TTAA 2 cut(s) 683, 973
Tru9I TTAA 2 cut(s) 683, 973
TscAI CASTG 3 cut(s) 256, 309, 619
TseFI GTSAC 3 cut(s) 304, 614, 855
TseI GCWGC 3 cut(s) 389, 528, 605
Tsp45I GTSAC 3 cut(s) 304, 614, 855
TspDTI ATGAA 2 cut(s) 561, 880
TspRI CASTG 3 cut(s) 256, 309, 619
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 2 cut(s) 92, 919
XbaI TCTAGA 1 cut(s) 130
XceI RCATGY 1 cut(s) 159
XspI CTAG 4 cut(s) 131, 420, 602, 908
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.