RLG00000028401

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000446
Physical Location & Seq
Reverse (-)
22266638 .. 22268994
2357 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028401

Sequence Viewer

Length: 1191 bp
ATGGCCCTATTGCCAAACCTCCCAGATGTCCTGATCATCCAACCACCACCAGCCTTCATATCTTTCGAATCCGACTTCACCCAGAAGTTCAACATCCTCCGAGCATGGGAATCGCCACTCCCCCTGGACCAGTTCCTCACCACCCACGCCCAATCAGTCCATGCCCTCCTCTGCCGCGCCGAGACTCCGATCAACGCCGACGTCCTCCGGCTGCTACCGGCCCTGAAGATCGTGGTGTCCGCCAGCACCGGCGCCGACCAAGTGGACAAAGCCGAGTGCCGGCGGCGTGGGATTGCGGTGACCAACGCAGGAAATGCGTACGCGGAAGATGTTGCTGATATCGCTGTTGGGTTGTTCATCGATGTGCTTCGGAAGATTTCAGCAGCTGATTGGTATGTGAGAAAAGGGCTTTGGAGTAGCAATGGAGATTATCCTCTTGGTTCCAAGATAGGTGGAAAGCGAGTTGGTATTGTTGGATTGGGAAGCATTGGCTATGAAGTTGCCAAAAGACTCGAGGCCTTTGGCTGCAAGATCTCATACAACTCAAGAAGCGAAAGGCCGTCTTTGTCATACACCTTCTATTCCAATGTCAGTGAACTTGCAGCAGACAGTGACGCTCTCGTAATCTGTTGTGGGTTAACAGACCAAACCCGCCACATGATCAACAAGGAAGTCTTATCTGCATTGGGAAAAGATGGAGTGGTAGTTAATATAGGGCGCGGGGCAATTATTGATGAGAAAGAATTGGTCAAGTGTTTGGTGAATGGAGAGATTAGAGGTGCTGGTTTGGATGTGTATGAGAATGAGCCTCATATACCGAAAGAGCTCGTGGCAATGGACAACGTTGTTCTGTCTCCACATAAGGCTGTTTATACAGTCGAGTCTGTCAAGAAAGGAGGTGCGCAGTGTTACCCTGGTCGCTCAGAATATGGTCAGATTGAAGACCAAGGTTGGATGGTGACAAAGGCTACTGGAAGAGATCGTCGCGGCTGCTGGGAGAGACAACGTGGTCGACGGAGCAAGCTTTTTGGTGGGAGGTTGTGCTGTTCAAGCTTGGGCCATTTGAACATTGTCTGTGTCATGGCTAGGACATTTCGTGGATTGGGTTCTATCAAACCTAGTGCTTGGGGTTTAGATCTGACCTATATTGGGTTGGATTTTTTGCTTGGGCTGCTCAACAAGTATAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

397

Amino Acids

43.29

Weight (kDa)

8.67

Isoelectric Point (pI)

40.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 46 - 229 3.8e-19 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 117 - 289 5.2e-51 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1008
AatII GACGTC 1 cut(s) 204
Acc16I TGCGCA 1 cut(s) 903
AccB1I GGYRCC 1 cut(s) 251
AccI GTMKAC 1 cut(s) 1012
AccII CGCG 4 cut(s) 177, 323, 720, 987
AciI CCGC 8 cut(s) 175, 240, 283, 296, 323, 652, 720, 987
AclI AACGTT 1 cut(s) 843
AcuI CTGAAG 1 cut(s) 245
AcyI GRCGYC 2 cut(s) 201, 252
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 3 cut(s) 106, 279, 1149
AgsI TTSAA 4 cut(s) 91, 941, 1050, 1066
AjnI CCWGG 2 cut(s) 123, 913
AjuI GAANNNNNNNTTGG 2 cut(s) 394, 426
AluBI AGCT 4 cut(s) 386, 826, 1024, 1053
AluI AGCT 4 cut(s) 386, 826, 1024, 1053
Alw21I GWGCWC 1 cut(s) 828
Alw26I GTCTC 3 cut(s) 176, 858, 994
AlwNI CAGNNNCTG 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 5 cut(s) 3, 219, 516, 557, 1057
ApeKI GCWGC 6 cut(s) 211, 383, 525, 602, 990, 1171
AspLEI GCGC 4 cut(s) 179, 254, 720, 904
AspS9I GGNCC 4 cut(s) 4, 127, 220, 1057
AsuHPI GGTGA 5 cut(s) 70, 130, 310, 772, 970
AsuII TTCGAA 1 cut(s) 66
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 1 cut(s) 127
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 1 cut(s) 828
BauI CACGAG 1 cut(s) 827
BbsI GAAGAC 1 cut(s) 948
Bbv12I GWGCWC 1 cut(s) 828
BbvI GCAGC 6 cut(s) 198, 395, 512, 614, 977, 1158
BccI CCATC 2 cut(s) 689, 949
BceAI ACGGC 1 cut(s) 544
BcgI CGANNNNNNTGC 2 cut(s) 93, 127
BciT130I CCWGG 2 cut(s) 125, 915
BclI TGATCA 2 cut(s) 33, 660
BcoDI GTCTC 3 cut(s) 176, 858, 994
BfaI CTAG 2 cut(s) 1086, 1119
BfoI RGCGCY 1 cut(s) 255
BglII AGATCT 2 cut(s) 531, 1135
BisI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 988, 991, 1172
BlsI GCNGC 9 cut(s) 176, 213, 285, 385, 527, 604, 989, 992, 1173
Bme1390I CCNGG 2 cut(s) 125, 915
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 4 cut(s) 4, 127, 220, 1057
BmiI GGNNCC 2 cut(s) 253, 442
BmrFI CCNGG 2 cut(s) 125, 915
BpiI GAAGAC 1 cut(s) 948
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 529
Bsa29I ATCGAT 1 cut(s) 360
BsaHI GRCGYC 2 cut(s) 201, 252
BsaJI CCNNGG 3 cut(s) 123, 913, 946
BsaXI ACNNNNNCTCC 2 cut(s) 690, 720
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 279, 1149
Bse118I RCCGGY 3 cut(s) 217, 248, 279
Bse1I ACTGG 2 cut(s) 130, 976
Bse3DI GCAATG 2 cut(s) 427, 840
BseBI CCWGG 2 cut(s) 125, 915
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 3 cut(s) 123, 913, 946
BseGI GGATG 4 cut(s) 36, 93, 796, 960
BseLI CCNNNNNNNGG 3 cut(s) 106, 279, 1149
BseMI GCAATG 2 cut(s) 427, 840
BseMII CTCAG 1 cut(s) 936
BseNI ACTGG 2 cut(s) 130, 976
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 6 cut(s) 198, 395, 512, 614, 977, 1158
BseYI CCCAGC 1 cut(s) 993
Bsh1236I CGCG 4 cut(s) 177, 323, 720, 987
BshFI GGCC 5 cut(s) 5, 221, 518, 559, 1059
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 360
BsiHKAI GWGCWC 1 cut(s) 828
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 4 cut(s) 208, 218, 249, 280
BsiWI CGTACG 1 cut(s) 318
BslI CCNNNNNNNGG 3 cut(s) 106, 279, 1149
BsmAI GTCTC 3 cut(s) 176, 858, 994
BsnI GGCC 5 cut(s) 5, 221, 518, 559, 1059
BsoBI CYCGRG 1 cut(s) 512
Bsp119I TTCGAA 1 cut(s) 66
Bsp1286I GDGCHC 1 cut(s) 828
Bsp143I GATC 7 cut(s) 33, 189, 228, 531, 660, 979, 1135
BspACI CCGC 8 cut(s) 175, 240, 283, 296, 323, 652, 720, 987
BspANI GGCC 5 cut(s) 5, 221, 518, 559, 1059
BspCNI CTCAG 1 cut(s) 935
BspDI ATCGAT 1 cut(s) 360
BspFNI CGCG 4 cut(s) 177, 323, 720, 987
BspLI GGNNCC 2 cut(s) 253, 442
BspT104I TTCGAA 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 2 cut(s) 427, 840
BsrFI RCCGGY 3 cut(s) 217, 248, 279
BsrI ACTGG 2 cut(s) 130, 976
BssAI RCCGGY 3 cut(s) 217, 248, 279
BssECI CCNNGG 3 cut(s) 123, 913, 946
BssMI GATC 7 cut(s) 33, 189, 228, 531, 660, 979, 1135
BssNI GRCGYC 2 cut(s) 201, 252
BssSI CACGAG 1 cut(s) 827
BssT1I CCWWGG 1 cut(s) 946
Bst2BI CACGAG 1 cut(s) 827
Bst2UI CCWGG 2 cut(s) 125, 915
Bst4CI ACNGT 2 cut(s) 611, 877
Bst6I CTCTTC 1 cut(s) 970
BstACI GRCGYC 2 cut(s) 201, 252
BstAPI GCANNNNNTGC 1 cut(s) 314
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 3 cut(s) 244, 281, 1022
BstDEI CTNAG 1 cut(s) 922
BstEII GGTNACC 1 cut(s) 298
BstF5I GGATG 4 cut(s) 36, 93, 796, 960
BstFNI CGCG 4 cut(s) 177, 323, 720, 987
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 4 cut(s) 179, 254, 720, 904
BstKTI GATC 7 cut(s) 36, 192, 231, 534, 663, 982, 1138
BstMAI GTCTC 3 cut(s) 176, 858, 994
BstMBI GATC 7 cut(s) 33, 189, 228, 531, 660, 979, 1135
BstMWI GCNNNNNNNGC 4 cut(s) 314, 341, 1050, 1171
BstNI CCWGG 2 cut(s) 125, 915
BstPI GGTNACC 1 cut(s) 298
BstSCI CCNGG 2 cut(s) 123, 913
BstUI CGCG 4 cut(s) 177, 323, 720, 987
BstV1I GCAGC 6 cut(s) 198, 395, 512, 614, 977, 1158
BstV2I GAAGAC 1 cut(s) 948
BstX2I RGATCY 2 cut(s) 531, 1135
BstYI RGATCY 2 cut(s) 531, 1135
Bsu15I ATCGAT 1 cut(s) 360
BsuRI GGCC 5 cut(s) 5, 221, 518, 559, 1059
BsuTUI ATCGAT 1 cut(s) 360
BtsCI GGATG 4 cut(s) 36, 93, 796, 960
BtsI GCAGTG 1 cut(s) 911
BtsIMutI CAGTG 3 cut(s) 598, 616, 911
Cac8I GCNNGC 3 cut(s) 244, 281, 1022
CaiI CAGNNNCTG 1 cut(s) 386
CfoI GCGC 4 cut(s) 179, 254, 720, 904
Cfr10I RCCGGY 3 cut(s) 217, 248, 279
Cfr13I GGNCC 4 cut(s) 4, 127, 220, 1057
ClaI ATCGAT 1 cut(s) 360
CseI GACGC 1 cut(s) 623
Csp6I GTAC 1 cut(s) 319
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 4 cut(s) 105, 161, 658, 1081
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 1 cut(s) 922
DinI GGCGCC 1 cut(s) 253
DpnI GATC 7 cut(s) 35, 191, 230, 533, 662, 981, 1137
DpnII GATC 7 cut(s) 33, 189, 228, 531, 660, 979, 1135
DrdI GACNNNNNNGTC 1 cut(s) 1008
DseDI GACNNNNNNGTC 1 cut(s) 1008
Eam1104I CTCTTC 1 cut(s) 970
EarI CTCTTC 1 cut(s) 970
EciI GGCGGA 1 cut(s) 229
Ecl136II GAGCTC 1 cut(s) 826
Eco130I CCWWGG 1 cut(s) 946
Eco147I AGGCCT 1 cut(s) 518
Eco24I GRGCYC 1 cut(s) 828
Eco32I GATATC 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 826
Eco57I CTGAAG 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 512
Eco91I GGTNACC 1 cut(s) 298
EcoICRI GAGCTC 1 cut(s) 826
EcoO65I GGTNACC 1 cut(s) 298
EcoRII CCWGG 2 cut(s) 123, 913
EcoRV GATATC 1 cut(s) 340
EcoT14I CCWWGG 1 cut(s) 946
EcoT38I GRGCYC 1 cut(s) 828
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 946
FaeI CATG 4 cut(s) 108, 164, 661, 1084
FalI AAGNNNNNCTT 4 cut(s) 547, 579, 659, 691
FatI CATG 4 cut(s) 104, 160, 657, 1080
FauI CCCGC 2 cut(s) 659, 713
FbaI TGATCA 2 cut(s) 33, 660
FblI GTMKAC 1 cut(s) 1012
Fnu4HI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 988, 991, 1172
FokI GGATG 4 cut(s) 23, 80, 803, 967
FriOI GRGCYC 1 cut(s) 828
Fsp4HI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 988, 991, 1172
FspBI CTAG 2 cut(s) 1086, 1119
FspI TGCGCA 1 cut(s) 903
GlaI GCGC 4 cut(s) 178, 253, 719, 903
GluI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 988, 991, 1172
GsaI CCCAGC 1 cut(s) 997
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 5 cut(s) 5, 221, 518, 559, 1059
HapII CCGG 4 cut(s) 208, 218, 249, 280
HgaI GACGC 1 cut(s) 623
HhaI GCGC 4 cut(s) 179, 254, 720, 904
Hin1I GRCGYC 2 cut(s) 201, 252
Hin1II CATG 4 cut(s) 108, 164, 661, 1084
Hin6I GCGC 4 cut(s) 177, 252, 718, 902
HinP1I GCGC 4 cut(s) 177, 252, 718, 902
HincII GTYRAC 2 cut(s) 639, 1013
HindII GTYRAC 2 cut(s) 639, 1013
HindIII AAGCTT 2 cut(s) 1022, 1051
HinfI GANTC 5 cut(s) 68, 110, 184, 510, 881
HpaI GTTAAC 1 cut(s) 639
HpaII CCGG 4 cut(s) 208, 218, 249, 280
HphI GGTGA 5 cut(s) 70, 130, 310, 772, 970
Hpy166II GTNNAC 4 cut(s) 265, 596, 639, 1013
Hpy188I TCNGA 7 cut(s) 73, 101, 189, 372, 925, 936, 1140
Hpy188III TCNNGA 3 cut(s) 31, 546, 889
Hpy8I GTNNAC 4 cut(s) 265, 596, 639, 1013
Hpy99I CGWCG 3 cut(s) 203, 987, 1017
HpyAV CCTTC 2 cut(s) 64, 586
HpyCH4III ACNGT 2 cut(s) 611, 877
HpyCH4IV ACGT 3 cut(s) 201, 843, 1006
HpyCH4V TGCA 3 cut(s) 528, 602, 683
HpyF10VI GCNNNNNNNGC 4 cut(s) 314, 341, 1050, 1171
HpyF3I CTNAG 1 cut(s) 922
HpySE526I ACGT 3 cut(s) 201, 843, 1006
Hsp92I GRCGYC 2 cut(s) 201, 252
Hsp92II CATG 4 cut(s) 108, 164, 661, 1084
HspAI GCGC 4 cut(s) 177, 252, 718, 902
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 279
KroNI GCCGGC 1 cut(s) 281
Ksp22I TGATCA 2 cut(s) 33, 660
KspAI GTTAAC 1 cut(s) 639
Kzo9I GATC 7 cut(s) 33, 189, 228, 531, 660, 979, 1135
LmnI GCTCC 1 cut(s) 1017
Lsp1109I GCAGC 6 cut(s) 198, 395, 512, 614, 977, 1158
MaeI CTAG 2 cut(s) 1086, 1119
MaeII ACGT 3 cut(s) 201, 843, 1006
MaeIII GTNAC 4 cut(s) 298, 611, 908, 958
MalI GATC 7 cut(s) 35, 191, 230, 533, 662, 981, 1137
MboI GATC 7 cut(s) 33, 189, 228, 531, 660, 979, 1135
MboII GAAGA 5 cut(s) 238, 338, 385, 953, 987
MflI RGATCY 2 cut(s) 531, 1135
MhlI GDGCHC 1 cut(s) 828
MluCI AATT 2 cut(s) 726, 743
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 3 cut(s) 178, 504, 890
MmeI TCCRAC 5 cut(s) 64, 96, 454, 932, 1134
MroNI GCCGGC 1 cut(s) 279
MseI TTAA 2 cut(s) 638, 708
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 4 cut(s) 208, 218, 249, 280
MspR9I CCNGG 2 cut(s) 125, 915
MvaI CCWGG 2 cut(s) 125, 915
MvnI CGCG 4 cut(s) 177, 323, 720, 987
MwoI GCNNNNNNNGC 4 cut(s) 314, 341, 1050, 1171
NaeI GCCGGC 1 cut(s) 281
NarI GGCGCC 1 cut(s) 252
NdeII GATC 7 cut(s) 33, 189, 228, 531, 660, 979, 1135
NgoMIV GCCGGC 1 cut(s) 279
NlaIII CATG 4 cut(s) 108, 164, 661, 1084
NlaIV GGNNCC 2 cut(s) 253, 442
NmeAIII GCCGAG 2 cut(s) 205, 298
NmuCI GTSAC 3 cut(s) 298, 611, 958
NsbI TGCGCA 1 cut(s) 903
NspV TTCGAA 1 cut(s) 66
PaeR7I CTCGAG 1 cut(s) 512
PceI AGGCCT 1 cut(s) 518
PdiI GCCGGC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 68, 110
Pfl23II CGTACG 1 cut(s) 318
PkrI GCNGC 9 cut(s) 176, 213, 285, 385, 527, 604, 989, 992, 1173
PleI GAGTC 3 cut(s) 178, 504, 889
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 3 cut(s) 178, 504, 889
Psp124BI GAGCTC 1 cut(s) 828
Psp1406I AACGTT 1 cut(s) 843
Psp6I CCWGG 2 cut(s) 123, 913
PspEI GGTNACC 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 993
PspGI CCWGG 2 cut(s) 123, 913
PspLI CGTACG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 253, 442
PspPI GGNCC 4 cut(s) 4, 127, 220, 1057
PspXI VCTCGAGB 1 cut(s) 512
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 2 cut(s) 531, 1135
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
RseI CAYNNNNRTG 1 cut(s) 362
SacI GAGCTC 1 cut(s) 828
SalI GTCGAC 1 cut(s) 1011
SaqAI TTAA 2 cut(s) 638, 708
SatI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 988, 991, 1172
Sau3AI GATC 7 cut(s) 33, 189, 228, 531, 660, 979, 1135
Sau96I GGNCC 4 cut(s) 4, 127, 220, 1057
SchI GAGTC 3 cut(s) 178, 504, 890
ScrFI CCNGG 2 cut(s) 125, 915
SduI GDGCHC 1 cut(s) 828
SfoI GGCGCC 1 cut(s) 253
Sfr274I CTCGAG 1 cut(s) 512
SfuI TTCGAA 1 cut(s) 66
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 1 cut(s) 127
SlaI CTCGAG 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 512, 544
SmoI CTYRAG 2 cut(s) 512, 544
Sse9I AATT 2 cut(s) 726, 743
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 8 cut(s) 175, 240, 283, 296, 323, 652, 720, 987
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 2 cut(s) 1086, 1119
SstI GAGCTC 1 cut(s) 828
StuI AGGCCT 1 cut(s) 518
StyD4I CCNGG 2 cut(s) 123, 913
StyI CCWWGG 1 cut(s) 946
TaaI ACNGT 2 cut(s) 611, 877
TaiI ACGT 3 cut(s) 204, 846, 1009
TaqI TCGA 5 cut(s) 66, 360, 513, 879, 1012
TasI AATT 2 cut(s) 726, 743
TauI GCSGC 3 cut(s) 177, 286, 990
TfiI GAWTC 2 cut(s) 68, 110
Tru1I TTAA 2 cut(s) 638, 708
Tru9I TTAA 2 cut(s) 638, 708
TscAI CASTG 3 cut(s) 598, 616, 911
TseFI GTSAC 3 cut(s) 298, 611, 958
TseI GCWGC 6 cut(s) 211, 383, 525, 602, 990, 1171
Tsp45I GTSAC 3 cut(s) 298, 611, 958
TspDTI ATGAA 3 cut(s) 46, 346, 510
TspGWI ACGGA 1 cut(s) 1030
TspRI CASTG 3 cut(s) 598, 616, 911
VpaK11BI GGWCC 1 cut(s) 127
XhoI CTCGAG 1 cut(s) 512
XmiI GTMKAC 1 cut(s) 1012
XspI CTAG 2 cut(s) 1086, 1119
ZraI GACGTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.