Rroxscaffold_7G00170140

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000446
Physical Location & Seq
Forward (+)
10592860 .. 10594657
1798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00170140.1

Sequence Viewer

Length: 969 bp
ATGGCCGAGTTGCTAGACCTGCCAGAAGTCCTGATTATCCAACCACCACCGCCCTTCACATCTTTCGAATCCGACTTCACCCAAAAGTTCAACATACTGCGAGCATGGGAATCACCTCTCCCTCTGGACCAGTTCCCCACCACCCACGCCCAATCAGTCCAGGCCCTCCTTAGCCACGTTTTCACTCTCGTCAACGGCGACCTCCTCCGGCTGCTACCGGCGCTGAAGCTCGTGGTGTCTGTCAGCACCGGCCCCAACCACGTGGACAAAGCTGAGTGCCTGAGGCGTGGGATTGCGGTGACCAATGCCGGAAATGCGTACTCGGAAGATGTTGCTGATATGGCTGTTGGGTTGTTCACCGATGTGAATCGGAGAATTTCAGCAGCTGATTCGTACGTCAGACGTGGGCTTTGGTCTAGCAAGGGAGACTATCCTCTTGGTTCCAAGATAGGTGGAAAGCGAGTAGGTATTGTTGGATTGGGAAACATTGGCTATGAAGTTGCGAAAAGACTTGAGGCCTTTGGCTGCAAGATCTCATACAACTCAAGGAGCGAAAGGCCGTCTTTGTCATACCCCTTCTATTCCAATGTCTGTGAGCTTGCAGCTAATAGTGATGCCCTCATCATCTGTTGTGCATTGACAGACCAAACCCGCCACATGATCAACAAGGAAGTCTTATCTGCGTTGGGAAAAGATGGGGTGGTAGTTAATATAGGGCGTGGGGCAATTATTGATGAGAAAGAATTGGTCAAGTGTTTGGTGAATGGAGAGATCAGAGGTGCTGGTTTGGATGTGTTTGAGAATGAGCCTCATATACCGAAAGAGCTTGTGGCAATGGACAATGTTGTTCTGTCTCCACATAAGGCTGCTCTTACCCTAGAGTCTTTCAAGAGAGGAAGTGAAGTAATGATCGCCAATTTGGAAGCTTTCTTCTCCAACAAACCCTTGGTTACTCCAGTCATGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

322

Amino Acids

35.04

Weight (kDa)

5.76

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 50 - 320 2e-17 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 117 - 289 2e-51 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 27
AciI CCGC 3 cut(s) 50, 296, 652
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 375
AcuI CTGAAG 1 cut(s) 245
AcvI CACGTG 1 cut(s) 262
AfaI GTAC 2 cut(s) 320, 395
AgsI TTSAA 2 cut(s) 91, 889
AjiI CACGTC 1 cut(s) 404
AjnI CCWGG 1 cut(s) 159
AleI CACNNNNGTG 1 cut(s) 362
AluBI AGCT 7 cut(s) 229, 272, 386, 598, 605, 826, 926
AluI AGCT 7 cut(s) 229, 272, 386, 598, 605, 826, 926
Alw26I GTCTC 2 cut(s) 420, 858
AlwNI CAGNNNCTG 1 cut(s) 386
AoxI GGCC 5 cut(s) 3, 162, 250, 516, 557
ApeKI GCWGC 5 cut(s) 211, 383, 525, 602, 866
ApoI RAATTY 1 cut(s) 375
ArsI GACNNNNNNTTYG 2 cut(s) 393, 425
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 3 cut(s) 127, 163, 251
AsuHPI GGTGA 5 cut(s) 70, 105, 310, 349, 772
AsuII TTCGAA 1 cut(s) 66
AvaII GGWCC 1 cut(s) 127
AxyI CCTNAGG 1 cut(s) 281
BauI CACGAG 1 cut(s) 230
BbrPI CACGTG 1 cut(s) 262
BbvI GCAGC 5 cut(s) 198, 395, 512, 614, 853
BccI CCATC 1 cut(s) 689
BceAI ACGGC 2 cut(s) 211, 544
BcgI CGANNNNNNTGC 2 cut(s) 372, 406
BciT130I CCWGG 1 cut(s) 161
BclI TGATCA 1 cut(s) 660
BcoDI GTCTC 2 cut(s) 420, 858
BfaI CTAG 3 cut(s) 14, 417, 878
BfoI RGCGCY 1 cut(s) 224
BfuAI ACCTGC 1 cut(s) 27
BglII AGATCT 1 cut(s) 531
BisI GCNGC 5 cut(s) 212, 384, 526, 603, 867
BlsI GCNGC 5 cut(s) 213, 385, 527, 604, 868
Bme1390I CCNGG 1 cut(s) 161
Bme18I GGWCC 1 cut(s) 127
BmgBI CACGTC 1 cut(s) 404
BmgT120I GGNCC 3 cut(s) 127, 163, 251
BmiI GGNNCC 2 cut(s) 253, 442
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 604
BpmI CTGGAG 1 cut(s) 939
Bpu10I CCTNAGC 1 cut(s) 170
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 2 cut(s) 529, 533
BsaAI YACGTR 1 cut(s) 262
BsaBI GATNNNNATC 1 cut(s) 366
BsaJI CCNNGG 1 cut(s) 945
Bse118I RCCGGY 2 cut(s) 217, 248
Bse1I ACTGG 2 cut(s) 130, 956
Bse21I CCTNAGG 1 cut(s) 281
Bse3DI GCAATG 1 cut(s) 840
Bse8I GATNNNNATC 1 cut(s) 366
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 1 cut(s) 945
BseGI GGATG 1 cut(s) 796
BseJI GATNNNNATC 1 cut(s) 366
BseMI GCAATG 1 cut(s) 840
BseMII CTCAG 2 cut(s) 264, 272
BseNI ACTGG 2 cut(s) 130, 956
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 5 cut(s) 198, 395, 512, 614, 853
BshFI GGCC 5 cut(s) 5, 164, 252, 518, 559
BsiSI CCGG 4 cut(s) 208, 218, 249, 309
BsiWI CGTACG 1 cut(s) 393
BsmAI GTCTC 2 cut(s) 420, 858
BsnI GGCC 5 cut(s) 5, 164, 252, 518, 559
Bsp119I TTCGAA 1 cut(s) 66
Bsp143I GATC 4 cut(s) 531, 660, 771, 909
BspACI CCGC 3 cut(s) 50, 296, 652
BspANI GGCC 5 cut(s) 5, 164, 252, 518, 559
BspCNI CTCAG 2 cut(s) 265, 273
BspLI GGNNCC 2 cut(s) 253, 442
BspMI ACCTGC 1 cut(s) 27
BspT104I TTCGAA 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 840
BsrFI RCCGGY 2 cut(s) 217, 248
BsrI ACTGG 2 cut(s) 130, 956
BssAI RCCGGY 2 cut(s) 217, 248
BssECI CCNNGG 1 cut(s) 945
BssMI GATC 4 cut(s) 531, 660, 771, 909
BssSI CACGAG 1 cut(s) 230
BssT1I CCWWGG 1 cut(s) 945
Bst2BI CACGAG 1 cut(s) 230
Bst2UI CCWGG 1 cut(s) 161
BstBAI YACGTR 1 cut(s) 262
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 2 cut(s) 102, 600
BstDEI CTNAG 3 cut(s) 170, 273, 281
BstEII GGTNACC 1 cut(s) 298
BstF5I GGATG 1 cut(s) 796
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 4 cut(s) 534, 663, 774, 912
BstMAI GTCTC 2 cut(s) 420, 858
BstMBI GATC 4 cut(s) 531, 660, 771, 909
BstMWI GCNNNNNNNGC 4 cut(s) 19, 220, 314, 341
BstNI CCWGG 1 cut(s) 161
BstPI GGTNACC 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 159
BstV1I GCAGC 5 cut(s) 198, 395, 512, 614, 853
BstX2I RGATCY 1 cut(s) 531
BstXI CCANNNNNNTGG 1 cut(s) 262
BstYI RGATCY 1 cut(s) 531
Bsu36I CCTNAGG 1 cut(s) 281
BsuRI GGCC 5 cut(s) 5, 164, 252, 518, 559
BtrI CACGTC 1 cut(s) 404
BtsCI GGATG 1 cut(s) 796
BveI ACCTGC 1 cut(s) 27
Cac8I GCNNGC 2 cut(s) 102, 600
CaiI CAGNNNCTG 1 cut(s) 386
CfoI GCGC 1 cut(s) 223
Cfr10I RCCGGY 2 cut(s) 217, 248
Cfr13I GGNCC 3 cut(s) 127, 163, 251
Csp6I GTAC 2 cut(s) 319, 394
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 3 cut(s) 105, 658, 961
CviQI GTAC 2 cut(s) 319, 394
DdeI CTNAG 3 cut(s) 170, 273, 281
DpnI GATC 4 cut(s) 533, 662, 773, 911
DpnII GATC 4 cut(s) 531, 660, 771, 909
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 945
Eco147I AGGCCT 1 cut(s) 518
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 245
Eco72I CACGTG 1 cut(s) 262
Eco81I CCTNAGG 1 cut(s) 281
Eco91I GGTNACC 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 163
EcoO65I GGTNACC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 159
EcoT14I CCWWGG 1 cut(s) 945
ErhI CCWWGG 1 cut(s) 945
FaeI CATG 3 cut(s) 108, 661, 964
FalI AAGNNNNNCTT 4 cut(s) 547, 579, 659, 691
FatI CATG 3 cut(s) 104, 657, 960
FauI CCCGC 1 cut(s) 659
FbaI TGATCA 1 cut(s) 660
Fnu4HI GCNGC 5 cut(s) 212, 384, 526, 603, 867
FokI GGATG 1 cut(s) 803
Fsp4HI GCNGC 5 cut(s) 212, 384, 526, 603, 867
FspBI CTAG 3 cut(s) 14, 417, 878
GlaI GCGC 1 cut(s) 222
GluI GCNGC 5 cut(s) 212, 384, 526, 603, 867
GsuI CTGGAG 1 cut(s) 939
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 5 cut(s) 5, 164, 252, 518, 559
HapII CCGG 4 cut(s) 208, 218, 249, 309
HhaI GCGC 1 cut(s) 223
Hin1II CATG 3 cut(s) 108, 661, 964
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 924
HinfI GANTC 5 cut(s) 68, 110, 367, 389, 881
HpaII CCGG 4 cut(s) 208, 218, 249, 309
HphI GGTGA 5 cut(s) 70, 105, 310, 349, 772
Hpy166II GTNNAC 3 cut(s) 193, 265, 357
Hpy188I TCNGA 5 cut(s) 73, 325, 372, 401, 776
Hpy188III TCNNGA 3 cut(s) 31, 125, 889
Hpy8I GTNNAC 3 cut(s) 193, 265, 357
HpyAV CCTTC 2 cut(s) 64, 586
HpyCH4IV ACGT 4 cut(s) 177, 261, 396, 403
HpyCH4V TGCA 3 cut(s) 528, 602, 635
HpyF10VI GCNNNNNNNGC 4 cut(s) 19, 220, 314, 341
HpyF3I CTNAG 3 cut(s) 170, 273, 281
HpySE526I ACGT 4 cut(s) 177, 261, 396, 403
Hsp92II CATG 3 cut(s) 108, 661, 964
HspAI GCGC 1 cut(s) 221
Ksp22I TGATCA 1 cut(s) 660
Kzo9I GATC 4 cut(s) 531, 660, 771, 909
LmnI GCTCC 1 cut(s) 549
Lsp1109I GCAGC 5 cut(s) 198, 395, 512, 614, 853
LweI GCATC 1 cut(s) 604
MaeI CTAG 3 cut(s) 14, 417, 878
MaeII ACGT 4 cut(s) 177, 261, 396, 403
MaeIII GTNAC 2 cut(s) 298, 949
MalI GATC 4 cut(s) 533, 662, 773, 911
MboI GATC 4 cut(s) 531, 660, 771, 909
MboII GAAGA 2 cut(s) 338, 922
MflI RGATCY 1 cut(s) 531
MluCI AATT 4 cut(s) 375, 726, 743, 916
MlyI GAGTC 1 cut(s) 890
MmeI TCCRAC 4 cut(s) 64, 96, 454, 960
MseI TTAA 2 cut(s) 708, 967
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 4 cut(s) 208, 218, 249, 309
MspR9I CCNGG 1 cut(s) 161
MvaI CCWGG 1 cut(s) 161
MwoI GCNNNNNNNGC 4 cut(s) 19, 220, 314, 341
NdeII GATC 4 cut(s) 531, 660, 771, 909
NlaIII CATG 3 cut(s) 108, 661, 964
NlaIV GGNNCC 2 cut(s) 253, 442
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 298
NspV TTCGAA 1 cut(s) 66
OliI CACNNNNGTG 1 cut(s) 362
PceI AGGCCT 1 cut(s) 518
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PfeI GAWTC 4 cut(s) 68, 110, 367, 389
Pfl23II CGTACG 1 cut(s) 393
PkrI GCNGC 5 cut(s) 213, 385, 527, 604, 868
PleI GAGTC 1 cut(s) 889
PmaCI CACGTG 1 cut(s) 262
PmlI CACGTG 1 cut(s) 262
PpsI GAGTC 1 cut(s) 889
Ppu21I YACGTR 1 cut(s) 262
Psp6I CCWGG 1 cut(s) 159
PspCI CACGTG 1 cut(s) 262
PspEI GGTNACC 1 cut(s) 298
PspGI CCWGG 1 cut(s) 159
PspLI CGTACG 1 cut(s) 393
PspN4I GGNNCC 2 cut(s) 253, 442
PspPI GGNCC 3 cut(s) 127, 163, 251
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 1 cut(s) 531
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 2 cut(s) 320, 395
RsaNI GTAC 2 cut(s) 319, 394
RseI CAYNNNNRTG 1 cut(s) 362
SaqAI TTAA 2 cut(s) 708, 967
SatI GCNGC 5 cut(s) 212, 384, 526, 603, 867
Sau3AI GATC 4 cut(s) 531, 660, 771, 909
Sau96I GGNCC 3 cut(s) 127, 163, 251
SchI GAGTC 1 cut(s) 890
ScrFI CCNGG 1 cut(s) 161
SfaNI GCATC 1 cut(s) 604
SfuI TTCGAA 1 cut(s) 66
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 512, 544
SmoI CTYRAG 2 cut(s) 512, 544
Sse9I AATT 4 cut(s) 375, 726, 743, 916
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 3 cut(s) 50, 296, 652
SspMI CTAG 3 cut(s) 14, 417, 878
StuI AGGCCT 1 cut(s) 518
StyD4I CCNGG 1 cut(s) 159
StyI CCWWGG 1 cut(s) 945
TaiI ACGT 4 cut(s) 180, 264, 399, 406
TaqI TCGA 1 cut(s) 66
TasI AATT 4 cut(s) 375, 726, 743, 916
TfiI GAWTC 4 cut(s) 68, 110, 367, 389
Tru1I TTAA 2 cut(s) 708, 967
Tru9I TTAA 2 cut(s) 708, 967
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 5 cut(s) 211, 383, 525, 602, 866
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 1 cut(s) 510
VpaK11BI GGWCC 1 cut(s) 127
XapI RAATTY 1 cut(s) 375
XcmI CCANNNNNNNNNTGG 1 cut(s) 943
XspI CTAG 3 cut(s) 14, 417, 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.