Rh1BG204200

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000446
Physical Location & Seq
Forward (+)
32247899 .. 32249529
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG204200.1

Sequence Viewer

Length: 969 bp
ATGGCCCTATTGCCAAACTTCCCAGATGTCCTGATCATCAAACCACCACCGGCCTTCATATCTTTCGAATCCGACTTCACCCAGAAGTTCAACATCCTCCGAGCATGGGAATCACCACTCCCTCTGGACCAGTTCCTCACCACCCACGCCCAATCAGTCCAGGCCCTCCTTTGCCGCGCTGTGACTCCGGTCAACGCCAAACTCCTCCAGCTGCTACCGGCCCTGAAGGTCGTGGTGACTGTCAGCACCGGCGCCAATCACGTGGACAAAGCGGAGTGCCGGCGGCGTGGGATTGCTGTGGCCAATGCCGGAAATGCATACTCAGAAGATGTTGCTGATATCGCTGTTGGGTTGTTCATCGATGTGAATCGGAGAATTTCAGCAGCTGATTGGTATGTGAGACGTGGGCTTTGGTCTAGCAAAGGAGACTATCCTCTTGGTTCCAAGATAGGTGGAAAGCGTGTAGGTATTGTTGGATTGGGAAGCATTGGCTATGAAGTTGCCAAAAGACTTGAGGCCTTTGGCTGCAAGATCTCATACAACTCAAGGAGTGAAAGGCCATCTTTGTCATACCCCTTCTATTCCAATGTCTGTGAGCTTGCAGCTAATAATGATGCTCTCATTATCTGTTGTGCATTGACAGACCAAACCCGCCACATGATCAACAAGGAAGTCTTATCTGCATTGGGAAAAGATGGAGTGATAGTTAATATAGGGCGTGGGGCAATTATTGATGAGAAAGAATTGGTCAAGTGTTTGGTGAATGGAGAGATCAGAGGTGCTGGTTTGGATGTGTTTGAGAATGAGCCTCATATACCGAAAGACCTTGTGGCAATGGACAATGTTGTTCTGTCTCCACATAGGGCTGTTTATACAGTAGAGTCTGTCAAGAAAGGAAGTGAAGGAATGATCGCCAATTTGGAAGCTTTCTTCTCCAATAAACCGTTGATTACTCCAGTCTATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

322

Amino Acids

35.01

Weight (kDa)

8.1

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 46 - 320 1.6e-21 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 117 - 289 4.9e-50 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 251
AccB7I CCANNNNNTGG 1 cut(s) 962
AccII CGCG 1 cut(s) 177
AciI CCGC 4 cut(s) 175, 272, 283, 652
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 1 cut(s) 375
AcuI CTGAAG 1 cut(s) 245
AcvI CACGTG 1 cut(s) 262
AcyI GRCGYC 1 cut(s) 252
AfiI CCNNNNNNNGG 2 cut(s) 106, 962
AgsI TTSAA 1 cut(s) 91
AjiI CACGTC 1 cut(s) 404
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 5 cut(s) 211, 386, 598, 605, 926
AluI AGCT 5 cut(s) 211, 386, 598, 605, 926
Alw26I GTCTC 3 cut(s) 394, 420, 858
AlwNI CAGNNNCTG 1 cut(s) 386
AoxI GGCC 7 cut(s) 3, 51, 162, 219, 300, 516, 557
ApeKI GCWGC 4 cut(s) 211, 383, 525, 602
ApoI RAATTY 1 cut(s) 375
ArsI GACNNNNNNTTYG 2 cut(s) 393, 425
AspLEI GCGC 2 cut(s) 179, 254
AspS9I GGNCC 4 cut(s) 4, 127, 163, 220
AsuHPI GGTGA 5 cut(s) 70, 105, 130, 247, 772
AsuII TTCGAA 1 cut(s) 66
AvaII GGWCC 1 cut(s) 127
BalI TGGCCA 1 cut(s) 302
BanI GGYRCC 1 cut(s) 251
BbrPI CACGTG 1 cut(s) 262
BbvI GCAGC 4 cut(s) 198, 395, 512, 614
BccI CCATC 2 cut(s) 568, 689
BciT130I CCWGG 1 cut(s) 161
BclI TGATCA 2 cut(s) 33, 660
BcoDI GTCTC 3 cut(s) 394, 420, 858
BfaI CTAG 1 cut(s) 417
BfoI RGCGCY 1 cut(s) 255
BglII AGATCT 1 cut(s) 531
BisI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
BlsI GCNGC 6 cut(s) 176, 213, 285, 385, 527, 604
Bme1390I CCNGG 1 cut(s) 161
Bme18I GGWCC 1 cut(s) 127
BmgBI CACGTC 1 cut(s) 404
BmgT120I GGNCC 4 cut(s) 4, 127, 163, 220
BmiI GGNNCC 2 cut(s) 253, 442
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 604
BoxI GACNNNNGTC 1 cut(s) 188
BpmI CTGGAG 2 cut(s) 191, 939
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 2 cut(s) 529, 533
Bsa29I ATCGAT 1 cut(s) 360
BsaAI YACGTR 1 cut(s) 262
BsaBI GATNNNNATC 1 cut(s) 366
BsaHI GRCGYC 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 690, 720
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 962
Bse118I RCCGGY 4 cut(s) 49, 217, 248, 279
Bse1I ACTGG 2 cut(s) 130, 956
Bse3DI GCAATG 1 cut(s) 840
Bse8I GATNNNNATC 1 cut(s) 366
BseBI CCWGG 1 cut(s) 161
BseCI ATCGAT 1 cut(s) 360
BseGI GGATG 2 cut(s) 93, 796
BseJI GATNNNNATC 1 cut(s) 366
BseLI CCNNNNNNNGG 2 cut(s) 106, 962
BseMI GCAATG 1 cut(s) 840
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 2 cut(s) 130, 956
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 4 cut(s) 198, 395, 512, 614
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 7 cut(s) 5, 53, 164, 221, 302, 518, 559
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 360
BsiSI CCGG 6 cut(s) 50, 188, 218, 249, 280, 309
BslI CCNNNNNNNGG 2 cut(s) 106, 962
BsmAI GTCTC 3 cut(s) 394, 420, 858
BsmBI CGTCTC 1 cut(s) 394
BsnI GGCC 7 cut(s) 5, 53, 164, 221, 302, 518, 559
Bsp119I TTCGAA 1 cut(s) 66
Bsp143I GATC 5 cut(s) 33, 531, 660, 771, 909
BspACI CCGC 4 cut(s) 175, 272, 283, 652
BspANI GGCC 7 cut(s) 5, 53, 164, 221, 302, 518, 559
BspCNI CTCAG 1 cut(s) 335
BspDI ATCGAT 1 cut(s) 360
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 2 cut(s) 253, 442
BspT104I TTCGAA 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 840
BsrFI RCCGGY 4 cut(s) 49, 217, 248, 279
BsrI ACTGG 2 cut(s) 130, 956
BssAI RCCGGY 4 cut(s) 49, 217, 248, 279
BssMI GATC 5 cut(s) 33, 531, 660, 771, 909
BssNI GRCGYC 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 161
Bst4CI ACNGT 3 cut(s) 241, 877, 945
BstACI GRCGYC 1 cut(s) 252
BstBAI YACGTR 1 cut(s) 262
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 2 cut(s) 281, 600
BstDEI CTNAG 1 cut(s) 322
BstF5I GGATG 2 cut(s) 93, 796
BstFNI CGCG 1 cut(s) 177
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 2 cut(s) 179, 254
BstKTI GATC 5 cut(s) 36, 534, 663, 774, 912
BstMAI GTCTC 3 cut(s) 394, 420, 858
BstMBI GATC 5 cut(s) 33, 531, 660, 771, 909
BstMWI GCNNNNNNNGC 2 cut(s) 314, 341
BstNI CCWGG 1 cut(s) 161
BstPAI GACNNNNGTC 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 159
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 4 cut(s) 198, 395, 512, 614
BstX2I RGATCY 1 cut(s) 531
BstXI CCANNNNNNTGG 1 cut(s) 262
BstYI RGATCY 1 cut(s) 531
Bsu15I ATCGAT 1 cut(s) 360
BsuRI GGCC 7 cut(s) 5, 53, 164, 221, 302, 518, 559
BsuTUI ATCGAT 1 cut(s) 360
BtrI CACGTC 1 cut(s) 404
BtsCI GGATG 2 cut(s) 93, 796
Cac8I GCNNGC 2 cut(s) 281, 600
CaiI CAGNNNCTG 1 cut(s) 386
CfoI GCGC 2 cut(s) 179, 254
Cfr10I RCCGGY 4 cut(s) 49, 217, 248, 279
Cfr13I GGNCC 4 cut(s) 4, 127, 163, 220
ClaI ATCGAT 1 cut(s) 360
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 2 cut(s) 105, 658
DdeI CTNAG 1 cut(s) 322
DinI GGCGCC 1 cut(s) 253
DpnI GATC 5 cut(s) 35, 533, 662, 773, 911
DpnII GATC 5 cut(s) 33, 531, 660, 771, 909
EaeI YGGCCR 1 cut(s) 300
Eco147I AGGCCT 1 cut(s) 518
Eco32I GATATC 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 245
Eco72I CACGTG 1 cut(s) 262
EcoO109I RGGNCCY 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 159
EcoRV GATATC 1 cut(s) 340
EcoT22I ATGCAT 1 cut(s) 319
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
Esp3I CGTCTC 1 cut(s) 394
FaeI CATG 2 cut(s) 108, 661
FalI AAGNNNNNCTT 4 cut(s) 547, 579, 659, 691
FatI CATG 2 cut(s) 104, 657
FauI CCCGC 1 cut(s) 659
FbaI TGATCA 2 cut(s) 33, 660
Fnu4HI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
FokI GGATG 2 cut(s) 80, 803
Fsp4HI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
FspBI CTAG 1 cut(s) 417
GlaI GCGC 2 cut(s) 178, 253
GluI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
GsuI CTGGAG 2 cut(s) 191, 939
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 7 cut(s) 5, 53, 164, 221, 302, 518, 559
HapII CCGG 6 cut(s) 50, 188, 218, 249, 280, 309
HhaI GCGC 2 cut(s) 179, 254
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 2 cut(s) 108, 661
Hin6I GCGC 2 cut(s) 177, 252
HinP1I GCGC 2 cut(s) 177, 252
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 924
HinfI GANTC 5 cut(s) 68, 110, 184, 367, 881
HpaII CCGG 6 cut(s) 50, 188, 218, 249, 280, 309
HphI GGTGA 5 cut(s) 70, 105, 130, 247, 772
Hpy166II GTNNAC 2 cut(s) 193, 265
Hpy188I TCNGA 5 cut(s) 73, 101, 325, 372, 776
Hpy188III TCNNGA 3 cut(s) 31, 125, 889
Hpy8I GTNNAC 2 cut(s) 193, 265
HpyAV CCTTC 4 cut(s) 64, 220, 586, 896
HpyCH4III ACNGT 3 cut(s) 241, 877, 945
HpyCH4IV ACGT 2 cut(s) 261, 403
HpyCH4V TGCA 5 cut(s) 317, 528, 602, 635, 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 314, 341
HpyF3I CTNAG 1 cut(s) 322
HpySE526I ACGT 2 cut(s) 261, 403
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 2 cut(s) 108, 661
HspAI GCGC 2 cut(s) 177, 252
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 279
KroNI GCCGGC 1 cut(s) 281
Ksp22I TGATCA 2 cut(s) 33, 660
Kzo9I GATC 5 cut(s) 33, 531, 660, 771, 909
Lsp1109I GCAGC 4 cut(s) 198, 395, 512, 614
LweI GCATC 1 cut(s) 604
MaeI CTAG 1 cut(s) 417
MaeII ACGT 2 cut(s) 261, 403
MaeIII GTNAC 2 cut(s) 181, 235
MalI GATC 5 cut(s) 35, 533, 662, 773, 911
MboI GATC 5 cut(s) 33, 531, 660, 771, 909
MboII GAAGA 2 cut(s) 338, 922
MflI RGATCY 1 cut(s) 531
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 4 cut(s) 375, 726, 743, 916
MluNI TGGCCA 1 cut(s) 302
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 2 cut(s) 178, 890
MmeI TCCRAC 2 cut(s) 96, 454
MnlI CCTC 9 cut(s) 107, 132, 146, 176, 215, 444, 508, 770, 819
Mox20I TGGCCA 1 cut(s) 302
Mph1103I ATGCAT 1 cut(s) 319
MroNI GCCGGC 1 cut(s) 279
MscI TGGCCA 1 cut(s) 302
MseI TTAA 1 cut(s) 708
MslI CAYNNNNRTG 1 cut(s) 362
Msp20I TGGCCA 1 cut(s) 302
MspA1I CMGCKG 2 cut(s) 211, 386
MspI CCGG 6 cut(s) 50, 188, 218, 249, 280, 309
MspR9I CCNGG 1 cut(s) 161
MvaI CCWGG 1 cut(s) 161
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 2 cut(s) 314, 341
NaeI GCCGGC 1 cut(s) 281
NarI GGCGCC 1 cut(s) 252
NdeII GATC 5 cut(s) 33, 531, 660, 771, 909
NgoMIV GCCGGC 1 cut(s) 279
NlaIII CATG 2 cut(s) 108, 661
NlaIV GGNNCC 2 cut(s) 253, 442
NmuCI GTSAC 2 cut(s) 181, 235
NsiI ATGCAT 1 cut(s) 319
NspV TTCGAA 1 cut(s) 66
PceI AGGCCT 1 cut(s) 518
PdiI GCCGGC 1 cut(s) 281
PfeI GAWTC 3 cut(s) 68, 110, 367
PflMI CCANNNNNTGG 1 cut(s) 962
PkrI GCNGC 6 cut(s) 176, 213, 285, 385, 527, 604
PleI GAGTC 2 cut(s) 178, 889
PluTI GGCGCC 1 cut(s) 255
PmaCI CACGTG 1 cut(s) 262
PmlI CACGTG 1 cut(s) 262
PpsI GAGTC 2 cut(s) 178, 889
Ppu21I YACGTR 1 cut(s) 262
PshAI GACNNNNGTC 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 159
PspCI CACGTG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 2 cut(s) 253, 442
PspPI GGNCC 4 cut(s) 4, 127, 163, 220
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 1 cut(s) 531
PvuII CAGCTG 2 cut(s) 211, 386
RseI CAYNNNNRTG 1 cut(s) 362
SaqAI TTAA 1 cut(s) 708
SatI GCNGC 6 cut(s) 175, 212, 284, 384, 526, 603
Sau3AI GATC 5 cut(s) 33, 531, 660, 771, 909
Sau96I GGNCC 4 cut(s) 4, 127, 163, 220
SchI GAGTC 2 cut(s) 178, 890
ScrFI CCNGG 1 cut(s) 161
SfaNI GCATC 1 cut(s) 604
SfoI GGCGCC 1 cut(s) 253
SfuI TTCGAA 1 cut(s) 66
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 512, 544
SmoI CTYRAG 2 cut(s) 512, 544
Sse9I AATT 4 cut(s) 375, 726, 743, 916
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 4 cut(s) 175, 272, 283, 652
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 1 cut(s) 417
StuI AGGCCT 1 cut(s) 518
StyD4I CCNGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 241, 877, 945
TaiI ACGT 2 cut(s) 264, 406
TaqI TCGA 2 cut(s) 66, 360
TasI AATT 4 cut(s) 375, 726, 743, 916
TauI GCSGC 2 cut(s) 177, 286
TfiI GAWTC 3 cut(s) 68, 110, 367
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TseFI GTSAC 2 cut(s) 181, 235
TseI GCWGC 4 cut(s) 211, 383, 525, 602
Tsp45I GTSAC 2 cut(s) 181, 235
TspDTI ATGAA 3 cut(s) 46, 346, 510
Van91I CCANNNNNTGG 1 cut(s) 962
VpaK11BI GGWCC 1 cut(s) 127
XapI RAATTY 1 cut(s) 375
XspI CTAG 1 cut(s) 417
Zsp2I ATGCAT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.