RLG00000011555

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000446
Physical Location & Seq
Forward (+)
10508354 .. 10509607
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011555

Sequence Viewer

Length: 630 bp
ATGGCTGTTGGGTTGTTCATCGATGTGAATCGGAGAATTTCAGCAGCTGATTGGTACGTCAGACATGGGCTTTGGTCTAGCAAGGGAGACTATCCTCTTGGTTCCAAGATGGGTGGAAAGCGAGTAGGTATTGTTGGATTGGGAAACATTGGCTATGAAGTTGCCAAAATACTTGAGGGCTTTGGCTGCAAGATCTCATACAACTCAAGGAGCGAAAGGCCGTCTTTGTCATACCCCTTCTATTCCAATGTCCGTGAGCTTGCAGCTAATAGTGATGCCCTCATCATCTGTTGTGCATTGACAGACCAAACCCGCCGCATGATCAACAAGGAAGTCTTATCTGCGTTGGGAAAAGATGGGGCGGTAGTTAATATAGGGCGTGGGGCAATTATTGATGAGAAAGAATTGGTCAAGTGTTTGGTGAATGGAGAGATCAAAGGTGCTGGTTTGGATGTGTTTGAGAATGAGCCTCATATACCGAAAGAGCTTGTGGCAATGGACAATGTTGTTCTGTCTCCACATAAGGCTCCTCTTACCCTAGAGTCTTTCAAGAGAGGAAGTGAAGTAATGATCGCCAATTTGGAAGCTTTCTTCTCCAACAAACCTTTGGTTACTCCAGTCATGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

210

Amino Acids

22.77

Weight (kDa)

7.65

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 4 - 176 3.6e-50 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 313, 316, 362
AcsI RAATTY 1 cut(s) 36
AfaI GTAC 1 cut(s) 56
AgsI TTSAA 1 cut(s) 550
AluBI AGCT 5 cut(s) 47, 259, 266, 487, 587
AluI AGCT 5 cut(s) 47, 259, 266, 487, 587
Alw26I GTCTC 2 cut(s) 81, 519
AlwNI CAGNNNCTG 1 cut(s) 47
AoxI GGCC 1 cut(s) 218
ApeKI GCWGC 3 cut(s) 44, 186, 263
ApoI RAATTY 1 cut(s) 36
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
AsuHPI GGTGA 1 cut(s) 433
BbvI GCAGC 3 cut(s) 56, 173, 275
BccI CCATC 2 cut(s) 103, 350
BceAI ACGGC 1 cut(s) 205
BclI TGATCA 1 cut(s) 321
BcoDI GTCTC 2 cut(s) 81, 519
BfaI CTAG 2 cut(s) 78, 539
BglII AGATCT 1 cut(s) 192
BisI GCNGC 4 cut(s) 45, 187, 264, 316
BlsI GCNGC 4 cut(s) 46, 188, 265, 317
BmiI GGNNCC 2 cut(s) 103, 528
BmsI GCATC 1 cut(s) 265
BpmI CTGGAG 1 cut(s) 600
BpuEI CTTGAG 2 cut(s) 190, 194
Bsa29I ATCGAT 1 cut(s) 21
BsaBI GATNNNNATC 1 cut(s) 27
Bse1I ACTGG 1 cut(s) 617
Bse3DI GCAATG 1 cut(s) 501
Bse8I GATNNNNATC 1 cut(s) 27
BseCI ATCGAT 1 cut(s) 21
BseGI GGATG 1 cut(s) 457
BseJI GATNNNNATC 1 cut(s) 27
BseMI GCAATG 1 cut(s) 501
BseNI ACTGG 1 cut(s) 617
BseRI GAGGAG 1 cut(s) 519
BseXI GCAGC 3 cut(s) 56, 173, 275
BshFI GGCC 1 cut(s) 220
BshVI ATCGAT 1 cut(s) 21
BsmAI GTCTC 2 cut(s) 81, 519
BsnI GGCC 1 cut(s) 220
Bsp143I GATC 4 cut(s) 192, 321, 432, 570
BspACI CCGC 3 cut(s) 313, 316, 362
BspANI GGCC 1 cut(s) 220
BspDI ATCGAT 1 cut(s) 21
BspLI GGNNCC 2 cut(s) 103, 528
BsrDI GCAATG 1 cut(s) 501
BsrI ACTGG 1 cut(s) 617
BssMI GATC 4 cut(s) 192, 321, 432, 570
BstC8I GCNNGC 1 cut(s) 261
BstF5I GGATG 1 cut(s) 457
BstKTI GATC 4 cut(s) 195, 324, 435, 573
BstMAI GTCTC 2 cut(s) 81, 519
BstMBI GATC 4 cut(s) 192, 321, 432, 570
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstV1I GCAGC 3 cut(s) 56, 173, 275
BstX2I RGATCY 1 cut(s) 192
BstYI RGATCY 1 cut(s) 192
Bsu15I ATCGAT 1 cut(s) 21
BsuRI GGCC 1 cut(s) 220
BsuTUI ATCGAT 1 cut(s) 21
BtsCI GGATG 1 cut(s) 457
Cac8I GCNNGC 1 cut(s) 261
CaiI CAGNNNCTG 1 cut(s) 47
ClaI ATCGAT 1 cut(s) 21
Csp6I GTAC 1 cut(s) 55
CspCI CAANNNNNGTGG 2 cut(s) 94, 129
CviAII CATG 3 cut(s) 65, 319, 622
CviQI GTAC 1 cut(s) 55
DpnI GATC 4 cut(s) 194, 323, 434, 572
DpnII GATC 4 cut(s) 192, 321, 432, 570
FaeI CATG 3 cut(s) 68, 322, 625
FalI AAGNNNNNCTT 4 cut(s) 208, 240, 320, 352
FatI CATG 3 cut(s) 64, 318, 621
FauI CCCGC 1 cut(s) 320
FbaI TGATCA 1 cut(s) 321
Fnu4HI GCNGC 4 cut(s) 45, 187, 264, 316
FokI GGATG 1 cut(s) 464
Fsp4HI GCNGC 4 cut(s) 45, 187, 264, 316
FspBI CTAG 2 cut(s) 78, 539
GluI GCNGC 4 cut(s) 45, 187, 264, 316
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 1 cut(s) 220
Hin1II CATG 3 cut(s) 68, 322, 625
HindIII AAGCTT 1 cut(s) 585
HinfI GANTC 2 cut(s) 28, 542
HphI GGTGA 1 cut(s) 433
Hpy188I TCNGA 2 cut(s) 33, 62
Hpy188III TCNNGA 1 cut(s) 550
HpyAV CCTTC 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 3 cut(s) 189, 263, 296
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 3 cut(s) 68, 322, 625
Ksp22I TGATCA 1 cut(s) 321
Kzo9I GATC 4 cut(s) 192, 321, 432, 570
LmnI GCTCC 2 cut(s) 210, 532
LpnPI CCDG 1 cut(s) 429
Lsp1109I GCAGC 3 cut(s) 56, 173, 275
LweI GCATC 1 cut(s) 265
MaeI CTAG 2 cut(s) 78, 539
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 1 cut(s) 610
MalI GATC 4 cut(s) 194, 323, 434, 572
MboI GATC 4 cut(s) 192, 321, 432, 570
MboII GAAGA 1 cut(s) 583
MflI RGATCY 1 cut(s) 192
MluCI AATT 4 cut(s) 36, 387, 404, 577
MlyI GAGTC 1 cut(s) 551
MmeI TCCRAC 2 cut(s) 115, 621
MnlI CCTC 6 cut(s) 105, 169, 290, 480, 540, 548
MseI TTAA 2 cut(s) 369, 628
MslI CAYNNNNRTG 1 cut(s) 23
MspA1I CMGCKG 1 cut(s) 47
MwoI GCNNNNNNNGC 1 cut(s) 186
NdeII GATC 4 cut(s) 192, 321, 432, 570
NlaIII CATG 3 cut(s) 68, 322, 625
NlaIV GGNNCC 2 cut(s) 103, 528
PfeI GAWTC 1 cut(s) 28
PkrI GCNGC 4 cut(s) 46, 188, 265, 317
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
PspN4I GGNNCC 2 cut(s) 103, 528
PstNI CAGNNNCTG 1 cut(s) 47
PsuI RGATCY 1 cut(s) 192
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 2 cut(s) 369, 628
SatI GCNGC 4 cut(s) 45, 187, 264, 316
Sau3AI GATC 4 cut(s) 192, 321, 432, 570
SchI GAGTC 1 cut(s) 551
SetI ASST 9 cut(s) 49, 60, 130, 261, 268, 442, 489, 589, 607
SfaNI GCATC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 2 cut(s) 173, 205
SmoI CTYRAG 2 cut(s) 173, 205
Sse9I AATT 4 cut(s) 36, 387, 404, 577
SsiI CCGC 3 cut(s) 313, 316, 362
SspMI CTAG 2 cut(s) 78, 539
TaiI ACGT 1 cut(s) 60
TaqI TCGA 1 cut(s) 21
TasI AATT 4 cut(s) 36, 387, 404, 577
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 1 cut(s) 28
Tru1I TTAA 2 cut(s) 369, 628
Tru9I TTAA 2 cut(s) 369, 628
TseI GCWGC 3 cut(s) 44, 186, 263
TspDTI ATGAA 2 cut(s) 7, 171
TspGWI ACGGA 1 cut(s) 242
XapI RAATTY 1 cut(s) 36
XcmI CCANNNNNNNNNTGG 1 cut(s) 604
XspI CTAG 2 cut(s) 78, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.